proposal pptd

7/23/2019 Proposal Pptd

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BSM4991 FINAL YEAR PROJECTNURUL HALIZA BT ABDUL RAHMAN169264PM DR JANNA ONG ABDULLAH


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Assessment of theACCUMULATOR POTENTIAL

ofIpomoea aquatica

for Its Applicability in Treatment of PhenolContaminated Water


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1.

INTRODUCTION


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PHYTOREMEDIATION

According to Sharma & Pandey (2014)

phytoremediation is termed as the use of green plants, including grasses and woody

species, to remove, contain, or render harmless such environmental contaminants as

heavy metals, metalloids, trace elements, organic compounds, and radioactive

compounds in soil or water.


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TYPES OF PHYTOREMEDIATION

Phytoextraction

Phytodegradation

Phytostabilization

Mechanisms Rhizofiltration

Volatilization

(US National Risk Management Research Laboratory, 2000)


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PHENOL - OVERVIEW

Soluble in water, oil,

and most organicsolvents

Most hazardous

organic pollutants evenat low concentration

Basic raw material forherbicides, drugs,

paints, cosmetics andothers

Discharged fromvarious industries, such

as refineries, coalprocessing,

pharmaceuticals etc.

(Shohreh et.al.,2014)


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PHENOL HEALTH EFFECT

May disrupt the metabolic system in microorganism, human or

animal

ACUTE toxicityof phenol in human symptoms : dryness in throatand mouth, and dark colours of urine

CHRONICexposure symptoms : anderoxia, muscle pain,headache, gastrointestinal pain and eventually lead to cancer

(Abd Gami et al., 2014)


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CURRENT METHODS INSOIL/WATER TREATMENT

METHODS REFERENCES DRAWBACK

THERMAL

TREATMENT(Soil Contaminant) Hamberg, 2009

High temperaturescould sterilize or

inactivate soilmicroorganisms

SOIL FLUSHING(Removal of lead,cadmium, and zinc from

soils)

Martin et.al.,2004

Alter the soilproperties

OXIDATION WITH O3(OZONATION)(Organic andinorganic compounds)

Shohrehet.al.,2014

High O3 generationcost, rate of pollutant

removal is slow


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Ipomoea aquatica

Fast growing herbaceous vine (Zhang

et al., 2014)

Heavy metal accumulator

(Shuiping, 2003)

Nitrogen accumulator

(Li et al., 2007)

Cheap, rapid growth and widely used

for water pollution control

(Song et al., 2009)


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2.PROBLEM

STATEMENT


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PHENOL DISCHARGEFROMINDUSTRIES& AGRICULTURE

CONTAMINATEDTHE WATER SOURCE

Phenol can bring detrimental effects on human health.If there is no toxicological reactions towards

accumulated phenol, Ipomoea aquatica can potentiallybe used to as an efficient phenol remediator in

treating phenol contaminated water source.


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3.RESEARCH

HYPOTHESIS


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The histological study, peroxidase assay

and zebra fish developmental study isnot affected by the accumulated phenol.

No deformation in Ipomoea aquaticatissues and normal development ofzebra fish embryo is observed at the

maximal phenol concentration (0.03g/L)absorbable by the plant


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4.PURPOSE OF STUDY

/ OBJECTIVE


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To improve the quality of contaminated water sourcevia phytoremediation using Ipomoea aquatica

MAIN OBJECTIVE

SPECIFIC OBJECTIVESTo investigate the toxicological effect of phenol accumulated in

Ipomoea aquatica on the development of zebra fish embryo

To study the histological effects of Ipomoea aquaticagrown in distilled water spiked with phenol


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4.RESEARCH

METHODOLOGY


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METHODOLOGY OVERVIEW

Ipomoea

aquatica+ phenol

DEVELOPMENTALSTUDY ON ZEBRA FISH

EMBRO

- Toxicological effects onembryo development

HISTOLOGICALSTUDY

- Implication ofphenol on plant

tissues

ENZYMEANALYSIS

(PEROXIDASE)- Plays a role in

detoxicification ofphenol


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GROWING PLANTS

Phenol concentrations : 0.1g/L, 0.2g/L and 0.3g/L

Grown in a box that fits 30 plantlets (duplicated x2)

PHENOL ACCUMULATION

Phenol concentrations in water and plants are quantifiedusing 4 - AAP Assayafter 2 weeks of cultivation

Standard Graph of Phenol Assay

PEROXIDASE STUDY Method as described in Ibanez et al. (2011)

TOXICITY STUDY

Plant samples are extracted and dried

Plant extracts are supplied in zebra fish growth medium Zebra fish embryo developmental stages are observed

HISTOLOGICAL STUDY

Plant samples from each box are picked randomly to assessthe effect of phenol accumulation in various plant parts

i.e root,stems,leaves


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2015 2016September October Mid Oct November Mid Nov December January February March April May June

Proposal presentation

& submission

Planting ofIpomoea

aquatica

Harvesting

of plant & plant

extraction

Phenol quantification

in plant

Cultivation ofzebra fish embryo

Developmental study of

zebra fish embryo

Data collection & 1st

draft thesis preparation

2nddraft thesis

Final draft thesis

Thesis submission

Final

exam

GANTTCHART

Peroxidase study

Sample tissues preparation

& sectioning


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REFERENCES A. Martin, T., & V. Ruby, M. (2004). Review of In Situ RemediationTechnologies for Lead, Zinc, and Cadmium in Soil.doi:10.1002/rem.20011

Abd Gami, A., Shukor, M., Abdul Khalil, K., Dahalan, F., Khalid, A.,& Ahmad, S. (2014). Phenol and its toxicity: A short review.

Journal Of Environmental Microbiology And Toxicology, No.2, 11-23.

E. Pivetz, B., & G. Huling, S. In-Situ Chemical Oxidation. U.S.Environmental Protection Agency.

Hamberg, R. (2009). In situ and on-site soil remediationtechniques.

Li, M., Yue-Jin, W., Yua, Z., Sheng, G., & Yu, H. (2007). Nitrogenremoval from eutrophic water by floating-bedgrown waterspinach (Ipomoea aquatica Forsk.) with ion implantation. WaterResearch, 41, 3152 3158.


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REFERENCES National Risk Management Research Laboratory,.

(2000). Introduction to Phytoremediation(pp. 3-51). Ohio: NationalRisk Management Research Laboratory.

Shuiping, C. (2003). Heavy Metals in Plants and

Phytoremediation. Environ Sci & Pollut Res 10, 5, 335 - 340. Zhang, Q., Achal, V., Xu, Y., & Xiang, W. (2014). Aquaculture

wastewater quality improvement by water spinach(Ipomoeaaquatica Forsskal) floating bed and ecological benefitassessmentin ecological agriculture district.Aquacultural Engineering, 60, 4855.


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CREDITS

Special thanks to all the people who helped me with this proposal

preparation

PM Dr Janna Ong Abdullah, FYP supervisor

Lee Siew Yi, Postgraduate student
http://www.slidescarnival.com/


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THANKS!Any questions?

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