product overview -...

Product Overview April 2014

About BioCision

Develop products to address unmet standardization needs in pre-analytical sample handling, processing, storage and transport BioCision products improve pre-analytical standardization of temperature-sensitive bio-medical samples: •  Day to day •  User to user •  Lab to lab •  Site to site

“The lack of standardized, high-quality biospecimens has been widely recognized as one of the most significant roadblocks to the progress of cancer research.” - NIH / NCI / BBRB

Consistent. Reproducible. Standardized.

Cell Cryopreservation CoolCell products for

standardized, controlled-rate cell freezing

Sample Temperature Management

CoolRack® and CoolBox™ for reproducible preparation of

temperature-sensitive samples

Intuitive design. Proprietary advanced thermal management technology.

mmm

Current methods are inconsistent and non-reproducible

Cooling samples in ice or gel-filled cooling blocks introduces a variety of problems.

Ice cooling •  Variable sample temp •  Contamination •  Wet, illegible labels •  Non-reproducible method

Gel-filled coolers •  Highly variable sample temp

(edge effect) •  Short cooling duration •  Cannot use while re-charging

CoolProducts provide consistent and reproducible sample cooling and freezing

Using a thermo-conductive CoolRack® module as an interface between the ice and sample provides a consistent and reproducible cooling method. Eliminating ice with a CoolBox™ system further minimizes the risk of sample contamination and provides hours of consistent and reproducible ice-free cooling or freezing.

Performance test: A temperature probe was placed into a 2.0 mL cryogenic vial containing 1.0 mL of water. The tube was inserted into a CoolRack CF45 thermo-conductive module. The module was placed onto a ThermalTray platform in a 37°C water bath and allowed to equilibrate. The CoolRack CF45 module was then removed and placed onto dry ice and equilibrated to -78°C (0 - 15 minutes) and then returned to the water bath to re-equilibrate to 37°C (15 - 30 minutes). This experiment was repeated five consecutive times and temperature profiles were recorded. Conclusion: The CoolRack CF45 module showed identical cooling profiles and phase transition (orange circles) over five consecutive freeze-thaw cycles.

CoolRack modules ensure reproducible cooling and thawing profiles

Liquid Nitrogen Dry Ice Ice Water Bath

-150˚C -78˚C 0˚ to 4˚C 37˚C +

•  Adapts from ambient (25oC) to approx. -150oC in 12-14 minutes •  Samples are upright and organized as they flash freeze •  No direct contact between sample and LN2 •  Reproducible method

•  Adapts from ambient (25oC) to -78oC in 5-7 minutes •  No need for ethanol – cost savings, no hazardous waste, no “sunk” tubes •  Samples are upright and organized as they snap-freeze •  Same freezing rate as direct immersion into dry ice or alcohol slurry •  Reproducible method

•  Adapts from ambient (25çC) to <4çC in 60-90 seconds •  Samples stay dry, organized, uniform in temperature •  Labels do not get wet or illegible •  All samples +/- 0.1oC •  Reproducible method

•  Adapts to 37°C in approx. 10 minutes •  Using a ThermalTray platform and a CoolRack tube module, samples stay above water line – minimizing risk of contamination •  Samples remain dry and organized •  Uniform sample temperature •  Reproducible method

CoolRack, CoolSink, ThermalTray: Compatible with broad temperature range

Use for very cold or very hot applications (LN2 to >100°C). Samples protected from direct contact with temperature source.

CoolRack® tube modules •  Including SBS-format modules

* Patented

CoolSink® plate modules* •  Including SBS-format modules

ThermalTray™ platforms* •  For supporting CoolRack and CoolSink

modules in liquid

CoolRack, CoolSink, ThermalTray: Accommodates variety of tubes and plates

CoolBox XT & 2XT CoolBox MP CoolBox 30 XT StarterHolds Tubes Ɣ Ɣ Ɣ ƔHolds Plates Ɣ Ɣ Ɣ

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CoolBox™: Ice-free workstations

CoolBox: How it works

Base

CoolRack or CoolSink sample module

Cooling Cartridge(Pre-frozen in -20˚C

freezer)

Lid

Simply pre-freeze the Cooling Core or cartridge in a -20°C freezer, place it in the base of the CoolBox, and rest a CoolRack or CoolSink module on top.

CoolBox XT and 2XT CoolBox MP and 30

CoolBox and CoolRack: Accessories TruCool® ice pans and buckets •  Made of non-toxic EVA foam •  Non-skid bottom, insulative and non-sweating •  5 sizes, 7 colors

TruCool® microfuge tubes and cryogenic vials CryoCeps™ cryovial grippers •  Microfuge tubes feature patented “pull back” cap ensures

thumb never passes over open top minimizing contamination risk

•  Cryogenic vials feature unique thermally-fused gasket (replaces O-ring) and bar-codes for secure identification

•  CryoCeps grippers protect fingers from handling frozen vials and tubes

CoolProbe™ temperature monitors •  Monitor sample temperature while using CoolRack and

CoolSink modules in ice •  Fitted probes for CoolRack M, CoolRack CF(T) •  Universal probe for other CoolRack and CoolSink modules

If you currently use an ice bucket, gel-filled coolers, refrigerators, freezers, or water bath, CoolBox and CoolRack products will improve your processes. Ideal for:

•  Tissue / Cell culture •  Tissue dissections & snap-freezing •  Cell culture preparation and thawing

•  Protein / Proteomics •  Protein isolation, sonication, fractionation •  Trypsin digestion

CoolBox and CoolRack: Use for a variety of applications

•  Molecular biology •  PCR preparations •  RNA isolation •  Reagent cooling

•  Microbiology •  Bacteria and virus freezing

Visit www.biocision.com/application-summary for application assistance.

CoolCell FTS30

CoolCell CoolCell SV2

CoolCell 5ml LX CoolCell LX

For a variety of tubes and capacities. The new standard for passive cell cryopreservation.

CoolCell®: Alcohol-free cell freezing

•  Replaces alcohol-based containers such as “Mr. Frosty” •  Simply fill with vials and place -80oC freezer •  Radially-symmetric thermal exchange design ensures

uniform cooling profiles to all vial positions •  Composed of high-density materials used in aerospace

industry for lightweight & insulative properties •  Highly thermo-conductive alloy core •  Models include conduction and/or micro-convection air

flow control •  Equal or better results than programmable step-down

freezers and alcohol-based freezing containers

Patent Pending

Passive controlled-rate cell freezing container

CoolCell: How it works

Replaces “Mr. Frosty” and programmable freezers. No alcohol. No on-going maintenance or cost. No hazardous waste disposal.

“Mr. Frosty” is a trademark of Thermo Fisher Scientific.

Five consecutive freezing runs show extremely consistent thermal profiles.

CoolCell containers ensure reproducible freezing profiles

CoolCell Isopropanol Container Consistent freezing rate •  All vials have uniform freezing profiles

Inconsistent freezing rate •  Isopropanol degradation ensures variability •  Two circles of vials results in further non-uniform

heat removal

No isopropanol •  No maintenance •  No pre-cooling •  No hazardous waste

Requires Isopropanol •  Track uses so can replenish IPA every 5th use •  On-going maintenance •  Spill risk •  Pre-cool in refrigerator (safety issue)

No hidden cost •  Once you buy it, you’re done paying for it!!

> $350 per unit per year to maintain properly •  Isopropanol expense •  Hazardous waste disposal costs

Easy to use •  Cap removes easily when frozen •  Not cold to the touch when frozen, no gloves

needed •  Ready for reuse after 5 minute warm-up time

Difficult to handle •  Cap is difficult to open when frozen, unit is slippery

(cells are warming up) •  Protective glove required when handling frozen unit •  Unit is cold, slippery, breakable

Minimal impact on freezer •  1/3 the heat impact on freezer; nearby samples

remain unharmed

Impacts local freezer area •  Large heat capacity removed from alcohol impacts

nearby samples

CoolCell vs. “Mr. Frosty”

Cell Lines •  LnCap, HTB77, A549, others •  Data and testing done by Lonza (Germany)

Primary Cells •  Variety of primary cells tested by labs at UCSF and ZenBio

Stem Cells and Cell Therapy Products •  Testing and data performed by USCF (Bluestone lab), TxCell and others

See CoolCell data at: http://www.biocision.com/resources/coolcell-performance-data

CoolCell: Compatible with many cell types

Viability and post-thaw functionality as good or better than “Mr. Frosty” and programmable freezers.

XT Starter ice-free cooling platform •  Ideal for use in the hood where ice is forbidden •  XT Starter and CoolRack modules provide compact, ice-

free cooling for cryogenic and cell therapy vials

TruCool® Hinged CryoBoxes •  Patented hinged lid ensures lid is always put on

correctly, keeping markings in synch with cryovials •  Boxes for 1ml & 2ml vials, and for taller vials •  6 bright colors •  With or without LN2 drain holes

TruCool® cryogenic vials CryoCeps™ cryogenic vial grippers •  Patented fused gasket layer on vial closure ensures

leak-proof seal •  Bar-coded vials for secure sample identification •  CryoCeps* protect fingers when handling frozen vials

* Patent pending

Cell Cryopreservation: Accessories

Thank you!

For questions, contact us at: [email protected]

© 2014. BioCision, LLC. All rights reserved. BioCision, Standardizing Samples, CoolRack, CoolSink, ThermalTray, CoolBox, CoolCell, TruCool, CoolProbe, CryoCeps are designated trademarks owned by BioCision, LLC..

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