pro and eu cell structure
DESCRIPTION
Biotech 1TRANSCRIPT
Eukaryotic and Eukaryotic and Prokaryotic CellsProkaryotic Cells
AHMP 5406AHMP 5406
Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
Features All Cells ShareFeatures All Cells Share
Hereditary info stored in DNAHereditary info stored in DNAProteins are used as catalysts Proteins are used as catalysts DNA replication occurs through templated DNA replication occurs through templated polymerizationpolymerizationHereditary info transcribed into RNAHereditary info transcribed into RNARNA translated into proteinRNA translated into protein
Later we will discuss these things in Later we will discuss these things in greater detailgreater detail
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From DNA to ProteinFrom DNA to Protein(Why is this eukaryotic?)(Why is this eukaryotic?)
Prokaryotic cells:Prokaryotic cells:No membrane bound organellesNo membrane bound organelles
Simple genome organizationSimple genome organization
Genes usually do not have intronsGenes usually do not have introns
Between 500 and 4000 genesBetween 500 and 4000 genes M. genitaliumM. genitalium has 477 genes has 477 genes
Great degree of diversityGreat degree of diversity
UnicellularUnicellular
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MycoplasmaMycoplasma genitalium genitalium
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Eukaryotic cellsEukaryotic cells
All organelles are membrane-boundAll organelles are membrane-boundComplex genome organizationComplex genome organizationLarge genome sizeLarge genome sizeBetween 6,000 and 30,000 genesBetween 6,000 and 30,000 genesGenes have intronsGenes have introns Where is the genetic diversity?Where is the genetic diversity?
Large amount of regulatory DNALarge amount of regulatory DNA To control gene expression To control gene expression
High degree of genetic redundancyHigh degree of genetic redundancyUnicellular or multicellularUnicellular or multicellular
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Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
General eukaryotic cellGeneral eukaryotic cell
1 and 2
Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
Cells can be powered by different Cells can be powered by different energy sourcesenergy sources
I eat other living things!I eat other living things! OrganotrophicOrganotrophic
AnimalsAnimals Protists etc. Protists etc.
I need a tan?I need a tan? PhototrohpicPhototrohpic
BacteriaBacteria PlantsPlants AlgaeAlgae
I eat dirt?I eat dirt? LithotropicLithotropic
ArcaebacteriaArcaebacteria
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Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
Eukaryotic cell may have been Eukaryotic cell may have been predatorspredators
The primordial eukaryotic cell may have The primordial eukaryotic cell may have survived by eating other cellssurvived by eating other cells
This would require:This would require: A large cellA large cell A flexible membraneA flexible membrane An elaborate cytoskeletonAn elaborate cytoskeleton Protected DNA (in an organelle, which one?)Protected DNA (in an organelle, which one?)
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4 Dinidium is a cilliated protozoan
Lunch!
He looks hungry
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Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
Genome organization in Prok. and Genome organization in Prok. and Euk.Euk.
ProkaryotesProkaryotes Circular DNACircular DNA
mtDNA very similarmtDNA very similar
EukaryotesEukaryotes Linear DNALinear DNA nuclear DNAnuclear DNA
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Prokaryotic DNAProkaryotic DNA
Eukaryotic DNAEukaryotic DNA
DNA packaged in a DNA packaged in a chromosomechromosome
Linear DNALinear DNA
Associated proteinsAssociated proteins
More details laterMore details later
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Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
Ways to generate new genes from preexisting genesWays to generate new genes from preexisting genes
Intragenic mutationIntragenic mutation Simply a mutation in the sequenceSimply a mutation in the sequence
Gene duplicationGene duplication Whole gene is just recopied into different part of the genomeWhole gene is just recopied into different part of the genome
DNA segment shufflingDNA segment shuffling Similar genes may exchange segmentsSimilar genes may exchange segments
Horizontal transferHorizontal transfer Cell to cell transfer of genetic materialCell to cell transfer of genetic material
e.g. Plasmidse.g. Plasmidsancient mitochondrial genesancient mitochondrial genesVirusesVirusesSexSex
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Gene FamiliesGene Families
Created by generation of different genes Created by generation of different genes from a precursorfrom a precursor
Speciation results in orthologous genesSpeciation results in orthologous genes An organismal phylogeny is producedAn organismal phylogeny is produced
Gene duplication can result in paralogous Gene duplication can result in paralogous genesgenes A gene phylogeny is producedA gene phylogeny is produced
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Objectives:Objectives:
1.1. Discuss the key differences between prokaryotic and Discuss the key differences between prokaryotic and eukaryotic cellular componentseukaryotic cellular components
2.2. Describe the basic structure of mammalian eukaryotic Describe the basic structure of mammalian eukaryotic cellscells
3.3. Discuss the different nutritional classesDiscuss the different nutritional classes4.4. Explain the endosymbiotic theory and development of Explain the endosymbiotic theory and development of
organelles in the Euk. cellorganelles in the Euk. cell5.5. Compare and contrast the genome organization of Compare and contrast the genome organization of
prokaryotes versus eukaryotesprokaryotes versus eukaryotes6.6. Compare the different ways new genes are generated Compare the different ways new genes are generated
from existing genesfrom existing genes7.7. Explain why the human genome is complexExplain why the human genome is complex
The human genome is complex:The human genome is complex:7
Partly because of duplications
Regulatory Regulatory DNA is DNA is
everywhere everywhere in eukaryotic in eukaryotic
genomesgenomes
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mRNA StructuremRNA Structure7
7Comparative Genome Sizes