Study of metal fluorescence enhancement using electron beam lithography patterned surfaces and DNA Origami structures

Nguyet Le, Dr. Timothy Corrigan and Ina Nikolli Concord University, Division of Natural Sciences, Athens WV 24712

David Neff and Dr. Michael Norton. Marshall University, Chemistry Department, Huntington WV 25701

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Fluorescence occurs when a molecule or substance absorbs light at one wavelength (color) and then re-emits light at a longer wavelength (different color). Used in several biomedical sciences:- Imaging cells- Determine protein and DNA structures - Examining antibodies for developing pharmaceuticals

Introduction

AcknowledgementsThanks to West Virginia Trust Fund to fund this research.Thank to SURE program of Marshall University for funding this research.

References 1.T.D. Corrigan, S.-H. Gou, R. J. Phaneuf, and H. Szmacinski, Appl. Phys. Lett. 88, 101112 (2006).2. T.D. Corrigan, S.-H. Gou, R. J. Phaneuf, and H. Szmacinski, J. fluoresc. 15, 777-784 (2005).3. Quantum Dots. Pharmainfo.net.2007. April 8th, 2011. http://www.pharmainfo.net/reviews/quantum-dots-novel-technique-drug-delivery-and-therapy.4. Carsten Sonnichsen, Plasmons in metal nanostructures. June 20th 2001 http://edoc.ub.uni-muenchen.de/2367/1/Soennichsen_Carsten.pdf77 (2005).

First approach: Silicon wafer with different size and spacing of gold nanoparticles was created using e-beam lithography. The chemistry and data results are shown below.

Experimental Procedure

ResultsQuantum dots are tiny semiconductor nanocrystals that give off fluorescence when they are stimulated by light. The purpose of this research is to increase fluorescence using these quantum dots in the presence of gold nanoparticles using beam lithography patterned surfaces.

A second approach taken using DNA Origami as structures to control spacing and distance of the Au particles with the quantum dots. Single stranded DNA has the ability to recognize complementary single DNA strands using Watson-Crick base pairing, to which QD are added.

Second Approach: 3x3 cross shaped DNA Origami nanopattern was synthesized using approximately 200 short sample DNA structures which are further to attached to AgNP and QD.

38 nm (remeasure)

30 nm AuNPs

~16.3 nm

~16.3 nm

~64.4 nm center to center, 34.4 nm edge to edge

30nm AuNPs on one dimensional cross origami - AuNPs located at CO3970 staples 99/104 and 163/168

~100 nm

~64.4 nm

~72nm

~136nm~210nm

5’- ……staple……….TAATAA TAATAATAA TAATAATAA AAAA CGCC AAGCTT GCAT- 3’

ss42m new 2014 monothiol 5’- /5ThioMC6-D/ATGC AAGCTT GGCG TTTT TTA TTA TTA TTA TTA TTA TTA TTA-3’

DNA Origami on a 1X1 crossed shape has been synthesized using (ingredients). The synthesis of DNA Origami structures were confirmed using the AFM. The next step includes the synthesis of 3x3 crossed shaped DNA origami structures in collaboration will Marshall University .

Following picture shows the entire DNA Sequence and the particular “stample strands’ used to hold the scaffold in place. Considering that the bases as constrained by design and period of dna helix, aptamers here project 'up' out of the plane of the origami arms as showncolored rings which simply indicate approximate tether staple positions

~5.5nm

~5.5nm

a

~25nm-calibrated in X

Rothemund/Neff/Hong UP out of page positions TBA (this indicates that the positions of TBAs out of plane of co3970 were checked against the Rothemund work and checked with Hong)

CO-3970each base pair =.34nm