present project 2007_1
TRANSCRIPT
Researchers Jariya Jiriyasin Jittikan Narapan
Sinnapa Vanduangden
AdvisorAdvisor Mr. Sorachai Saelim Mr. Sorachai Saelim
IntroductionIntroduction
• Treatment of acne and other skin eruptions such as sores, (Aburjai T, Phytotherapy Research 2003)
• Important for normalizing oily/acne skin and balances dry skin (Myra Michelle Eby, 2006)
(http://www.comav.upv.es/taxonomy_intro.html)
(http://your-cosmetics.com) (http://cyborg9.exteen.com/20070518/entry)
• To extract the oil from Cucurbita moschata Decne seeds
• To produce liquid soap from Cucurbita moschata Decne seed oil
• To test the quality of liquid soap
ObjectiveObjective
ExperimentsExperiments
1. Extraction of the oil from Cucurbita moschata Decne seeds by Soxhlet extractor
3. Test the quality of liquid soap2. Production of liquid soap
Measure the foam capacity
Measure the pH value
Test the ability of emulsifying paraffin oil
Studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+
Studying the reaction of liquid soap with inorganic acid
Measuring the satisfaction of using the liquid soap
Experiments
1. Extraction of the oil by Soxhlet extractor1. Extraction of the oil by Soxhlet extractor
ResultsResults
Table 1. Show the character and quantity of Cucurbita moschata Decne seed oil
Weight of the seeds (g)
Quantity of the oil (ml)
Physical characteristic of the oil
picture
770 235.8 The oil is liquid and quite sticky. It has green-brown colors
2. Production of liquid soap2. Production of liquid soap
Oils
Dissolve KOH in water
The oils were mixed from the formulas
Table 2. Show the ratio of Cucurbita moschata Decne seed oil to other oils that used to produce liquid soap (% by mass)
formulaOils
1 2 3 4 5 6 7 8 9
Coconut oil 40 30 30 50 50 - 100 - -
Palm oil 30 40 30 50 - 50 - 100 -
Pumpkin seed oil
30 30 40 - 50 50 - - 100
2. Production of liquid soap2. Production of liquid soap
(1) (2) (3)
(6)
(5)(4)
(40:30:30) (50:50:0) (50:0:50)
(0:50:50)
(30:30:40)(30:40:30)
(7)
(100:0:0)
(8)
(0:100:0)
(9)
(0:0:100)
PS (Coconut oil : Palm oil : Pumpkin seed oil)
Produced Liquid soapsProduced Liquid soaps
Measure the foam capacity
Measure the pH value
Test the ability of emulsifying paraffin oil
Studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+
Studying the reaction of liquid soap with inorganic acid
Measure the satisfactions by using liquid soaps
3. Test the quality of liquid soap3. Test the quality of liquid soap
3. Test the quality of liquid soapTest the quality of liquid soap
Weighed liquid soap from each formula 5 g
Dissolve in 50 ml - DO water
Test the quality of liquid soap Liquid soap solutions
Preparation of the solution of liquid soapPreparation of the solution of liquid soap
Add each solution of liquid soap into test tube, 4 ml per tube
Shake each test tube
Observe and write down the results
Measure the foam capacityMeasure the foam capacity
1.852
2.25 2.252.4
2.25
2.652.55
2.9
4
3.35
2.8
0
0.5
1
1.5
2
2.5
3
3.5
4
1 2 3 4 5 6 7 8 9 10 11 12
123456789101112
Results of measuring foam capacityResults of measuring foam capacity
Chart 1. Show the results of measuring foam capacity
Measure the pH value of each solution of liquid soap by pH - meter
The pH value of each solution was written down
Adjust pH meter by buffer solution
Measure the pH valueMeasure the pH value
Results of measuring the pH valueResults of measuring the pH value
pH
formulas
Chart 2. Show the results of measuring the pH value
10.1 10.12 10.11 10.07
10.36
10.63
9.73
10.7710.53
10.12 10.11
9.6
8
8.5
9
9.5
10
10.5
1 2 3 4 5 6 7 8 9 10 11 12
123456789101112
Add paraffin oil into two test tubes, 10 drops per tube
Add the solution of the 1st formula of liquid soap into test tubes, 5 ml per tube then shake
The results of testing the ability of emulsifying paraffin oil were observed and written down
Do this experiment to each solution of liquid soap
Test the ability of emulsifying paraffin oilTest the ability of emulsifying paraffin oil
Results of emulsifying paraffin oil testResults of emulsifying paraffin oil test
Formula The results
DO water The solution has no color, there is the oil layer on the surface 5 mm high. It separates obviously
Formula 1 The solution has clear yellow color, there is the oil layer between the foam and the solution 2 mm high
Formula 2 The solution has light yellow color, it is turbid, there is the oil layer between the foam and the solution 3 mm high
Formula 3 The solution has clear yellow color, there is the oil layer between the foam and the solution 1.75 mm high
Table 3. Show the results of emulsifying paraffin oil test
Formula The results
Formula 4 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2.5 mm high
Formula 5 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.5 mm high
Formula 6 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2 mm high
Formula 7 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2 mm high
Formula 8 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2 mm high
Results of emulsifying paraffin oil testResults of emulsifying paraffin oil test
Formula The results
Formula 9 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.75 mm high
Formula 10 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 2.5 mm high
Formula 11 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.5 mm high
Formula 12 The solution has white color, it is turbid, there is the oil layer between the foam and the solution 1.5 mm high
Results of emulsifying paraffin oil testResults of emulsifying paraffin oil test
The
results
were
written
down
Liquid soap solution
CaCl2
MgCl2
FeCl3
Paraffin oil
Studying the reaction of liquid soapStudying the reaction of liquid soap with Cawith Ca2+2+, , MgMg2+2+ and Fe and Fe3+3+
The
results
were
written
down
ResultsResults
Formula Reagents
1 % CaCl2 1 % MgCl2 1 % FeCl3
1 there are white dregs. there is no dreg in the tube. there is a big piece of brown-red dregs.
2 there are turbid white dregs. there are turbid white dregs.
there is a big piece of brick-red dregs.
3 there are turbid white dregs. there are turbid white dregs.
there is a big piece of brick-red dregs.
4 there are turbid white dregs. there are turbid white dregs.
there are turbid orange dregs.
5 there are soft yellow dregs. there are soft yellow dregs. there are turbid orange dregs.
6 there are turbid white dregs. there are soft yellow dregs. there are turbid orange dregs.
Table 4. Show the results of studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+
Formula Reagents
1 % CaCl2 1 % MgCl2 1 % FeCl3
7 there are white dregs. there are white dregs. there are orange dregs.
8 there are turbid white dregs. there are turbid white dregs.
there are turbid orange dregs.
9 there are soft yellow dregs. there are soft yellow dregs. there is no dregs.
10 there is no dregs. there is no dregs. there are turbid orange dregs.
11 there are turbid white dregs. there are turbid white dregs.
there are turbid orange dregs.
12 there is no dregs. there are turbid white dregs.
there are turbid orange dregs.
ResultsResults
ResultsResults
Table 5. Show the results of studying the reaction of liquid soap with Ca2+, Mg2+ and Fe3+ then with paraffin oil
Formula Reagents
1 % CaCl2 1 % MgCl2 1 % FeCl3
1 there is oil layer. there is oil layer. there is a round piece of fat and dregs.
2 there is oil layer. there is a round piece of fat and dregs.
there is a round piece of fat and dregs.
3 there is oil layer. there is a round piece of fat and dregs.
there is oil layer.
4 there is no oil layer. there is oil layer. there is a round piece of fat and dregs.
5 there is oil layer. there is oil layer. there is a round piece of fat and dregs.
Formula Reagents
1 % CaCl2 1 % MgCl2 1 % FeCl3
6 there is a round piece of fat and dregs.
there is a round piece of fat and dregs.
there is oil layer.
7 there is oil layer. there is no oil layer. there is a round piece of fat and dregs.
8 there is oil layer. there is oil layer. there is a round piece of fat and dregs.
9 there is oil layer. there is oil layer. there is oil layer.
10 there is oil layer. there is oil layer. there is oil layer.
11 there is oil layer. there is oil layer. there is oil layer.
12 there is oil layer. there is oil layer. there is oil layer.
ResultsResults
Studying the reaction of liquid soapStudying the reaction of liquid soap with inorganic acidwith inorganic acid
ResultsResults
Table 6. Show the results of studying the reaction of liquid soap with inorganic acid
Formula Process
Add HCl Add KOH Burning
1 there are dregs. dissolved in KOH there is no flame.
2 there are dregs. dissolved in KOH there is flame when burned.
3 there are dregs. dissolved in KOH there is no flame.
4 there is no dregs. there is no dregs. there is no flame.
5 there is no dregs. there is no dregs. there is no flame.
6 there are dregs. quite dissolved in KOH there is no flame.
Formula Process
Add HCl Add KOH Burning
7 there are dregs. quite dissolved in KOH there is no flame.
8 there are dregs. dissolved in KOH there is flame when burned.
9 there are dregs. dissolved in KOH there is no flame.
10 there are dregs. dissolved in KOH there is no flame.
11 there are dregs. dissolved in KOH there is no flame.
12 there are dregs. dissolved in KOH there is no flame.
ResultsResults
Measuring the satisfactions of using the liquid soapsMeasuring the satisfactions of using the liquid soaps
Measuring the satisfactions of using the liquid soaps by 90 MWIT students with questionnaires, then the results were
analyzed by SPSS 11.5
Section 1, Inquire the samples about personal information.
Section 2, Inquire the samples about the satisfactions when using the liquid soaps such as produced foam, smell, colors and cleaning.
Questionnaires
ResultsResults
Chart 3. Show the ages of samples Chart 4. Show the personal ailment of samples
Color Smell Foam Advertisement Cleaning Price Others Total
Frequency(%)
11(5.76)
69(36.13)
8(4.19)
33(17.28)
43(22.51)
20(10.47)
7(3.66)
191(100)
ResultsResults
Table 7. Show the behavior of buying soap of samples
the level of satisfactions points
good quality and great satisfactionsgood quality and good satisfactionsgood quality and fairly good satisfactionsbad quality and not satisfy
- 350.400.
250. - 3.4 9
150. - 2.4 9
1.00 - 1.49
Table 8. Show the standard of satisfied levels of liquid soaps
Measuring the satisfaction of using the liquid soapsMeasuring the satisfaction of using the liquid soaps
Chart 5. Show the averaged satisfactions of using the liquid soaps
ResultsResults
ConclusionConclusion
- From the extraction of oil in pumpkin seeds oil, the oil content is about 30.6 %
- From the experiments, the formula 10 has the most foam capacity in compare with all the liquid soap and the formula 9 has the most foam capacity in compare with 9 formulas produced liquid soap.
- The formula 5, 11, 12 has the most ability to emulsify paraffin oil. As the paraffin oil is lipid with composed in dirt.
ConclusionConclusion
- From the experiment of measuring pH value, the formula 7 has the least pH value in compare with 9 formulas produced liquid soap, which is 9.73.
- From the measuring of satisfactions value of using liquid soap, the formula 7 , 8 and 9 satisfied the samples respectively.
AcknowledgementAcknowledgement
• Mr. Sorachai Saelim
• Department of Chemistry, Mahidol Wittayanusorn school
ReferencesReferences
African Journal of Biotechnology Vol. 4 (1), pp. 36-44, January 2005
Aburjai T, Natsheh FM. Plants used in cosmetics. Phytotherapy Research 2003;17:987–1000
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