practical pharmacology-1 (po701) third year faculty of pharmacy department of pharmacology &...

PRACTICAL PHARMACOLOGY-1 (Po701) Third Year

FACULTY OF PHARMACY

Department of Pharmacology & Toxicology

CAIRO UNIVERSITY

شعارالكلية مصر في الصيدلي التعليم منارة

اإلقليمي ومحيطها

Determination of the site of action of

some skeletal muscle stimulants on

Isolated Frog Rectus Abdominis

Why Ach contracts the skeletal muscle?

Why carbachol contracts the skeletal muscle?

Why K + contracts the skeletal muscle?

Aim:Determining the following properties of an unknown drug which causes skeletal muscle contraction:

1. Its susceptibility to destruction by cholinesterase enzyme (CE).

2. Its site of action (acts on Nm receptors or directly on the muscle).

Qualitative experiment

Unknown drug Susceptibility to CE enzyme

Action on Nm receptors

Cholinester (e.g. Ach) + +Carbamate ester(e.g. carbachol) _ +Direct skeletal muscle stimulant (e.g. K+) _ _

☺How to test for susceptibility of the drug to

destruction by CE?

Using anti-cholinesterase drug:

Incubate the muscle with PHYSOSTIGMINE (anti-cholinesterase) then add the drug:

if it is hydrolysed by CE if it is not hydrolysed by CE

PHYSOSTIGMINE will prevent its destruction

↑ its amount at receptor site potentiation of muscle response

PHYSOSTIGMINE will not change muscle response

☺How to find out the site of action of the drug?

Block Nm receptors by TUBOCURARINE (neuromuscular blocker), then add the drug:

if it acts on Nm receptors

muscle response

will ↓

if it doesn't act on Nm receptors

muscle response will not change

In this experiment:

The muscle’s response to the drug before & after adding the experimental tools (PHYSOSTIGMINR, & TUBOCURARINE) is observed.

Q. Can we add the SM dose as a reference dose only once at the beginning of the experiment?

Ans. No, because the sensitivity of the muscle may change during the experiment leading to changes in muscle response.

0.2 U

0.2 U

0.2 U

0.2 U

0.4 U

0.8 U

0.2 U

0.2 AntiChEs (Physostigmine)

5 min.

OFF + NO WASH after

0.2 NMB (Tubocurarine)

5 min.

OFF + NO WASH after

1. Susceptibility to destruction by cholinesterase: Not susceptible

2. Site of action: Direct

Unknown may be K+

0.2 U

0.4 U

0.8 U

0.2 U

0.2 U

0.2 U

0.2 U


5 min.


5 min.

OFF + NO WASH after

OFF + NO WASH after

1. Susceptibility to destruction by cholinesterase: Not susceptible

2. Site of action: Stimulates muscular Nm receptors

Unknown may be Carbachol

0.2 U

0.4 U

0.8 U

0.2 U

0.2 U

0.2 U

0.2 U


5 min.


5 min.

OFF + NO WASH after

OFF + NO WASH after

1. Susceptibility to destruction by cholinesterase: Susceptible

2. Site of action: Stimulates muscular Nm receptors

Unknown may be ACh

AChCarbacho

lK+

After Physostigmine No

changeNo

change

After Tubocurarine No

change

Interactive Computer Simulated Practical Pharmacology

Isolated Frog Rectus Abdominis

Experiment:Determination of the site of action of some skeletal muscle stimulants

STEPS

STEPS 1

STEPS 2

29 11 2014

STEPS 2

29

11 2014

Type code Select Lab time

STEPS 3

29 11 2014

STEPS 4

Type your name your ID

Press Start

STEPS 5

Press Start

STEPS 6

Press Add Dose

STEPS 7

STEPS 7

25

STEPS 8

Complete the D/R relationship for the given UNK. solution (3 graded doses only)

STEPS 8Effect of anticholinesterase “physostigmine”:1. Choose a submax. dose2. Repeat addition of submax. dose after the D/R3. Incubate rectus with “physostigmine” 4. Repeat addition of submax. on incubated rectus

Physostigmine

Tubocurarine

Physostigmine

STEPS 9Effect of neuromuscular blocker “tubocurarine”

Repeat the previous steps but with “tubocurarine”

STEPS 10

STEPS 11

Press printer icon to printWrite your conclusion in the printed chart

ΔΜϟΎΜϟ :Δϴγ έΪ ϟΔϗήϔϟ

ϡΎόϟϲ όϣΎΠϟ: 2014/ 2015 :ϰ γ έΪ ϟϞμ ϔϟβ ϣΎΨϟ

(PO 701) -ΔϳϭΩϷϢϠϋέήϘϣ1

Ϣδ ϗϷϡϮϤδ ϟϭΔϳϭΩ

ΩΎόϴϤϟ Ώϼτ ϟϡΎϗέ ϢϟϥΎϛ ϡϮϴϟ

8:30-8:40 odd

Group B

ϡϮϤ

δϟϭΔ

ϳϭΩϷ

ϞϣΎόϣ

(αΩΎδ

ϟέϭΪϟ)

ΖΒδ

ϟ

20/12/2014

8:50-9:00 even

11:30-11:40 odd

Group A

11:50-12:00 even

ϰ ϠϋΏϼτ ϟ ϊ ϳίϮΗϲ ϠϤόϟϱ ήψϧϥΎΤΘϣ

έήϘϤϟϖ δ Ϩϣ

Ω.ΐ ϴτ ΨϟϦϤϳ /

Pharmacology – I (PO 701)

The prelabs will be held in the pharmacology labs (sixth floor) before the ICDL labs

Time 9:45-10:15 (odd)

10:30-11

(even)

12:15 -12:45 (odd)

1-1:30 (even) Day Lab

Satu

rday

29/11-

6/1

2/2

014

ICDL Ground floor

˻ ˹ ˺ ˻ ˺ ̀ ˺ -˻ ˹ ˺ ˻ ˻ ˾ ˾

˻ ˹ ˺ ˻ ˺ ̀ ˺ -˻ ˹ ˺ ˻ ˻ ˾ ˾

˻ ˹ ˺ ˺ ˺ ˹ ́ -˻ ˹ ˺ ˺ ˺ ˽ ˾ -˻ ˹ ˺ ˺ ˺ ˽ ́ -2011248-˻ ˹ ˹ ́ ˺ ˻ ̂ -˻ ˹ ˺ ˺ ˹ ˹ ́ -

2012001-2012081

˻ ˹ ˺ ˺ ˺ ˹ ́-˻ ˹ ˺ ˺ ˺ ˽ ˾-˻ ˹ ˺ ˺ ˺ ˽ ́-2011248-˻ ˹ ˹ ́ ˺ ˻ ̂-˻ ˹ ˺ ˺ ˹ ˹ ́-

2012001-2012081

New ICDL lab Beside Clinical Program

Unit

2012256-2012341

2012256-2012341

2012082-2012170 2012082-2012170

FACULTY OF PHARMACY DEPARTMENT OF PHARMACOLOGY &

TOXICOLOGY

CAIRO UNIVERSITY Academic Year: 2014/2015

Semester: Five Clinical Pharmacy Program

Student’s Distribution in ICDL Labs


Student Numbers

2012171-2012255

(odd)

2012171-2012255

(even) 2012256-2012341

(odd)

2012256-2012341 (even) Day Hour

Satu

rday

13/1

2/2

014

1st Hour (9 am-10 am)

Pharmacology

Labs ( 6th floor)

ICDL (Ground floor)

Pharmacology Labs ( 6th floor)

New ICDL lab Beside Clinical

Program Unit

2nd Hour (10 am -11 am)

ICDL (Ground floor)

Pharmacology Labs ( 6th

floor)


Program Unit



TOXICOLOGY



Student’s Distribution in Labs 13/ 12/ 2014

COURSE COORDINATOR

PROF. DR. AIMAN S. EL-KHATIB


Student Numbers 2011108-2011145-2011148-2011248-2008129-2011008-2012001-2012081

(odd)

2011108-2011145-2011148-2011248-2008129-2011008-2012001-2012081

(even)

2012082-2012170

(odd)

2012082-2012170 (even)

Day Hour

Sat

urd

ay

13/1

2/2

014

1st Hour

(11:30am-12:30 pm)


ICDL (Ground floor) Pharmacology

Labs ( 6th floor)


Program Unit

2nd Hour (12:30 pm -1:30 pm)

ICDL (Ground floor)



Program Unit



TOXICOLOGY



COURSE COORDINATOR

PROF. DR. AIMAN S. EL-KHATIB

Student’s Distribution in Labs 13/ 12/ 2014

practical pharmacology-1 (po701) third year faculty of pharmacy department of pharmacology &...

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