ppt on published research paper
TRANSCRIPT
Seminar onRecent Trends in Pharmaceutical Sciences
(Research Paper Title Uptake and transport of furanodiene in Caco-2 cellmonolayers a comparison study between furanodiene and furanodiene loaded PLGA nanoparticles)
BY Nitin P Kanwale
Roll no 06M Pharm Sem III
Dept QATUNDER GUIDANCE OFDr Mrudula H Bele
(Pharmaceutics department)MVP Samajs college of Pharmacy Nashik
MVP Samajrsquos College of Pharmacy Nashik01052023 1
CONTENTS1 Drug profile2 Introduction3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-
NPs4 Characterization of FDE-PLGA-NPs And FDE-PEG-
PEGA-NPs5 Conclusion6 References
01052023 2
3
1DRUG PROFILE
FURANODIENE
Stucture
Chemical name(5E9E)-3610-trimethyl-47811-tetrahydrocyclodeca[b]furan
Pharmacological activities
Anti-tumor Anti-inflammation
01052023
4
2 INTRODUCTIONRESEARCH QUESTION As it possible to improve the stability and bioavailability of FDE by preparing FDE loaded PLGA and PEGylated PLGA nanoparticles
GOAL OF WORK furanodiene has some disadvantages like low aqueous solubility low stability low permeability uptake by cell so there is need to improve the stability and oral bioavailability of furanodiene
Use Caco-2 cell line as a in vitro model to evaluate the response of Caco-2 cells to FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs to compare the capacity of the carrier to mediate FDE transport across cell monolayers
01052023
3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPsbull By using modified spontaneous emulsion solvent diffusion
method
3 mg furanodiene+25 mg PLGA(PEG-PLGA) dissolved in 2 ml acetone slowelly injected into 15 ml of 02 tween80 solution magnetic stirring at room temp to evaporate the organic solvent
nanoparticle suspension was collected by centrifugation(15000 rpmmin) wash 3 times by using deionized water to remove excessive emulsifier nanoparticle suspension then freeze dried to obtain a fine power of nanoparticle
01052023 5
4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPsbull 41Nanoparticle size size distribution zeta potential surface
morphology entrapment efficiency EE= (T-F) Ttimes100 Where T= total amount of furanodiene added F= free furanodiene amount in the supernatant Table1 Characteristics of the nanoparticles
Fig 1 Scanning electron microscope (SEM) images of the nanoparticles Scale bar = 200 nm (a) FDE-PLGA-NPs (b) FDE-PEG-PLGA-NPs
01052023 6
42 In vitro stability studies
01052023 7
Fig 2 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA- Fig 3 Stabilities of FDE FDE-PLGA-NPs and FDE- NPs in PBS (pH 74) PEG-PLGA-NPs in artificial gastric fluids
Fig 4 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial intestinal fluids
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
CONTENTS1 Drug profile2 Introduction3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-
NPs4 Characterization of FDE-PLGA-NPs And FDE-PEG-
PEGA-NPs5 Conclusion6 References
01052023 2
3
1DRUG PROFILE
FURANODIENE
Stucture
Chemical name(5E9E)-3610-trimethyl-47811-tetrahydrocyclodeca[b]furan
Pharmacological activities
Anti-tumor Anti-inflammation
01052023
4
2 INTRODUCTIONRESEARCH QUESTION As it possible to improve the stability and bioavailability of FDE by preparing FDE loaded PLGA and PEGylated PLGA nanoparticles
GOAL OF WORK furanodiene has some disadvantages like low aqueous solubility low stability low permeability uptake by cell so there is need to improve the stability and oral bioavailability of furanodiene
Use Caco-2 cell line as a in vitro model to evaluate the response of Caco-2 cells to FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs to compare the capacity of the carrier to mediate FDE transport across cell monolayers
01052023
3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPsbull By using modified spontaneous emulsion solvent diffusion
method
3 mg furanodiene+25 mg PLGA(PEG-PLGA) dissolved in 2 ml acetone slowelly injected into 15 ml of 02 tween80 solution magnetic stirring at room temp to evaporate the organic solvent
nanoparticle suspension was collected by centrifugation(15000 rpmmin) wash 3 times by using deionized water to remove excessive emulsifier nanoparticle suspension then freeze dried to obtain a fine power of nanoparticle
01052023 5
4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPsbull 41Nanoparticle size size distribution zeta potential surface
morphology entrapment efficiency EE= (T-F) Ttimes100 Where T= total amount of furanodiene added F= free furanodiene amount in the supernatant Table1 Characteristics of the nanoparticles
Fig 1 Scanning electron microscope (SEM) images of the nanoparticles Scale bar = 200 nm (a) FDE-PLGA-NPs (b) FDE-PEG-PLGA-NPs
01052023 6
42 In vitro stability studies
01052023 7
Fig 2 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA- Fig 3 Stabilities of FDE FDE-PLGA-NPs and FDE- NPs in PBS (pH 74) PEG-PLGA-NPs in artificial gastric fluids
Fig 4 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial intestinal fluids
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
3
1DRUG PROFILE
FURANODIENE
Stucture
Chemical name(5E9E)-3610-trimethyl-47811-tetrahydrocyclodeca[b]furan
Pharmacological activities
Anti-tumor Anti-inflammation
01052023
4
2 INTRODUCTIONRESEARCH QUESTION As it possible to improve the stability and bioavailability of FDE by preparing FDE loaded PLGA and PEGylated PLGA nanoparticles
GOAL OF WORK furanodiene has some disadvantages like low aqueous solubility low stability low permeability uptake by cell so there is need to improve the stability and oral bioavailability of furanodiene
Use Caco-2 cell line as a in vitro model to evaluate the response of Caco-2 cells to FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs to compare the capacity of the carrier to mediate FDE transport across cell monolayers
01052023
3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPsbull By using modified spontaneous emulsion solvent diffusion
method
3 mg furanodiene+25 mg PLGA(PEG-PLGA) dissolved in 2 ml acetone slowelly injected into 15 ml of 02 tween80 solution magnetic stirring at room temp to evaporate the organic solvent
nanoparticle suspension was collected by centrifugation(15000 rpmmin) wash 3 times by using deionized water to remove excessive emulsifier nanoparticle suspension then freeze dried to obtain a fine power of nanoparticle
01052023 5
4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPsbull 41Nanoparticle size size distribution zeta potential surface
morphology entrapment efficiency EE= (T-F) Ttimes100 Where T= total amount of furanodiene added F= free furanodiene amount in the supernatant Table1 Characteristics of the nanoparticles
Fig 1 Scanning electron microscope (SEM) images of the nanoparticles Scale bar = 200 nm (a) FDE-PLGA-NPs (b) FDE-PEG-PLGA-NPs
01052023 6
42 In vitro stability studies
01052023 7
Fig 2 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA- Fig 3 Stabilities of FDE FDE-PLGA-NPs and FDE- NPs in PBS (pH 74) PEG-PLGA-NPs in artificial gastric fluids
Fig 4 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial intestinal fluids
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
4
2 INTRODUCTIONRESEARCH QUESTION As it possible to improve the stability and bioavailability of FDE by preparing FDE loaded PLGA and PEGylated PLGA nanoparticles
GOAL OF WORK furanodiene has some disadvantages like low aqueous solubility low stability low permeability uptake by cell so there is need to improve the stability and oral bioavailability of furanodiene
Use Caco-2 cell line as a in vitro model to evaluate the response of Caco-2 cells to FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs to compare the capacity of the carrier to mediate FDE transport across cell monolayers
01052023
3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPsbull By using modified spontaneous emulsion solvent diffusion
method
3 mg furanodiene+25 mg PLGA(PEG-PLGA) dissolved in 2 ml acetone slowelly injected into 15 ml of 02 tween80 solution magnetic stirring at room temp to evaporate the organic solvent
nanoparticle suspension was collected by centrifugation(15000 rpmmin) wash 3 times by using deionized water to remove excessive emulsifier nanoparticle suspension then freeze dried to obtain a fine power of nanoparticle
01052023 5
4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPsbull 41Nanoparticle size size distribution zeta potential surface
morphology entrapment efficiency EE= (T-F) Ttimes100 Where T= total amount of furanodiene added F= free furanodiene amount in the supernatant Table1 Characteristics of the nanoparticles
Fig 1 Scanning electron microscope (SEM) images of the nanoparticles Scale bar = 200 nm (a) FDE-PLGA-NPs (b) FDE-PEG-PLGA-NPs
01052023 6
42 In vitro stability studies
01052023 7
Fig 2 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA- Fig 3 Stabilities of FDE FDE-PLGA-NPs and FDE- NPs in PBS (pH 74) PEG-PLGA-NPs in artificial gastric fluids
Fig 4 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial intestinal fluids
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPsbull By using modified spontaneous emulsion solvent diffusion
method
3 mg furanodiene+25 mg PLGA(PEG-PLGA) dissolved in 2 ml acetone slowelly injected into 15 ml of 02 tween80 solution magnetic stirring at room temp to evaporate the organic solvent
nanoparticle suspension was collected by centrifugation(15000 rpmmin) wash 3 times by using deionized water to remove excessive emulsifier nanoparticle suspension then freeze dried to obtain a fine power of nanoparticle
01052023 5
4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPsbull 41Nanoparticle size size distribution zeta potential surface
morphology entrapment efficiency EE= (T-F) Ttimes100 Where T= total amount of furanodiene added F= free furanodiene amount in the supernatant Table1 Characteristics of the nanoparticles
Fig 1 Scanning electron microscope (SEM) images of the nanoparticles Scale bar = 200 nm (a) FDE-PLGA-NPs (b) FDE-PEG-PLGA-NPs
01052023 6
42 In vitro stability studies
01052023 7
Fig 2 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA- Fig 3 Stabilities of FDE FDE-PLGA-NPs and FDE- NPs in PBS (pH 74) PEG-PLGA-NPs in artificial gastric fluids
Fig 4 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial intestinal fluids
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPsbull 41Nanoparticle size size distribution zeta potential surface
morphology entrapment efficiency EE= (T-F) Ttimes100 Where T= total amount of furanodiene added F= free furanodiene amount in the supernatant Table1 Characteristics of the nanoparticles
Fig 1 Scanning electron microscope (SEM) images of the nanoparticles Scale bar = 200 nm (a) FDE-PLGA-NPs (b) FDE-PEG-PLGA-NPs
01052023 6
42 In vitro stability studies
01052023 7
Fig 2 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA- Fig 3 Stabilities of FDE FDE-PLGA-NPs and FDE- NPs in PBS (pH 74) PEG-PLGA-NPs in artificial gastric fluids
Fig 4 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial intestinal fluids
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
42 In vitro stability studies
01052023 7
Fig 2 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA- Fig 3 Stabilities of FDE FDE-PLGA-NPs and FDE- NPs in PBS (pH 74) PEG-PLGA-NPs in artificial gastric fluids
Fig 4 Stabilities of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial intestinal fluids
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
43 Cytotoxicity test
Fig 5 The cytotoxicity of FDE FDE-PLGA-NPs and FDE-PEG-PLGA-NPs Caco-2 cells were incubated with sample solutions (1 to 40 μgminus1 of FDE) for 6 h Cell viability was determined by MTT assay
01052023 8
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
44 CELLULAR UPTAKE STUDY
Fig 6 Quantitative analysis of FDE cellular uptake Caco-2 cells were incubated with sample solutions (15 μgmLminus1 of FDE) for different durations Intracellular FDE was assayed by RP-HPLC
01052023 9
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
45 Transport study
Where Papp= Apparent permeability coefficient dq= amount of FDE in the receptor compartment at time t A = diffusion area C0 = initial concentration of FDE in the donor compartment
01052023 10
Papp
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
Fig 7 Transport of FDE across Caco-2 cell monolayers (AP to BL )
Fig 9 Transport of FDE across Caco-2 cell monolayers (BL to AP )
01052023 11
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
5CONCLUSIONThese results indicated that FDE-PLGA-NPs especially FDE-PEG-PLGA-NPs could enhance the stability and hydrophilicity of FDE and increase the permeation of FDE across Caco-2 cell monolayers
01052023 12
OVER VIEWPOSITIVE POINT
NEGATIVE POINT
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
6 REFERENCES1 Gang L Dong-hai L Xin-xin X Li-fang Q Jun-teng W Ke L
Uptake And Transport Of Furanodiene In Caco-2 Cell Monolayers A Comparison Study Between Furanodiene And Furanodiene Loaded PLGA Nanoparticles Chinese Journal Of Natural Medicines201311(1)049-055
01052023 13
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-
14
01052023
- Slide 1
- CONTENTS
- 1DRUG PROFILE
- 2 INTRODUCTION
- 3 Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-NPs
- 4 CHARACTERIZATION OF FDE-PLGA-NPs AND FDE-PEG-PEGA-NPs
- 42 In vitro stability studies
- 43 Cytotoxicity test
- 44 CELLULAR UPTAKE STUDY
- 45 Transport study
- Slide 11
- 5CONCLUSION
- 6 REFERENCES
- Slide 14
-