AB

ST

RA

CT

S

S4 Abstracts Heart, Lung and Circulation2007;16:S1–S201

(lung weight/body weight ratio [0.33 vs. 0.40, p < 0.05]).Cell culture experiments demonstrated a direct dosedependent reduction of collagen synthesis with FT-011 inresponse to AII and TGF-� stimulation (p < 0.01).Conclusion: Treatment with FT-011 post MI preventsadverse LV remodelling and heart failure at least in part byreducing pathological remodelling of collagen in the LV.

doi:10.1016/j.hlc.2007.06.011

6PKA/PKC cross-talk mediates NADPH oxidase-inducedNa+–K+ pump inhibition in cardiac myocytes

G.A. Figtree ∗, C. White, A. Garcia, E.J. Hamilton,H.H. Rasmussen

Department of Cardiology, Royal North Shore Hospital, Uni-versity of Sydney, Australia

Adrenergic Na+–K+ pump regulation is often attributedto direct phosphorylation of the pump molecule by cAMP-activated kinase (PKA), despite its poor access to putativephosphorylation sites. We have recently demonstratedthat adrenergic inhibition of the pump in cardiac myocytesis dependent on NADPH oxidase activation. As PKC� isknown to mediate NADPH oxidase activation, we havenow examined if adrenergic pump regulation is mediatedby PKC�. We voltage clamped and internally perfused rab-

FRIDAY 10 AUGUST

Affiliate Nursing

7Perception of Coronary Risk Factors in Patients FollowingPercutaneous Coronary Intervention

R. Fernandez ∗, P. Davidson, R. Griffiths, C. Juergens,Y. Salamonson

South Western Sydney Centre for Applied Nursing Research,Sydney, Australia

Aim: This presentation seeks to describe the perception ofcoronary risk factors in patients following percutaneouscoronary intervention (PCI).Background: People with diabetes, have a 2–3-foldincreased risk for coronary heart disease (CHD) and2–4-fold higher CHD morbidity and mortality rates. Percu-taneous coronary intervention is a commonly performedrevascularisation technique for coronary heart disease(CHD). A range of risk factor modification strategies arerequired to prevent CHD progression, particularly in highrisk groups such as diabetics.Methods: Prospective, consecutive participants (n = 270)undergoing PCI between April 2003 and March 2004 werefollowed up 1 year following PCI.

bit ventricular myocytes. Pipette solutions included 10 mMNa, and Na+–K+ pump current (IP), arising from the 3:2Na+:K+ exchange ratio, normalised for membrane capac-itance, was identified as the shift in current induced bypump inhibition with 100 �M ouabain. Adenylyl cyclasewas activated by 100 nM forskolin to mimic �1/�2 adren-ergic stimulation. IP of myocytes exposed to forskolin was38% lower than IP for controls (n = 10; p < 0.05). Inclusionof a peptide that selectively inhibits PKC� abolished theforskolin-induced inhibition of IP suggesting cross-talkbetween PKA and PKC�. To obtain independent confir-mation of such cross-talk we loaded myocytes with theoxidative fluorescent dye dihydroethidium (DHE) andexamined them using confocal fluorescent microscopy.Forskolin induced a 57% increase in DHE-fluorescencecompared with control (n = 7; p < 0.05). The increase wasabolished by preincubation with the NADPH oxidaseinhibitor apocynin; with pegylated superoxide dismutase,and with the myristolated PKC� inhibitory peptide. Weconclude that adrenergic activation of NADPH oxidasedepends on PKA/PKC� cross-talk, resulting in Na+–K+

pump inhibition in cardiac myocytes.

doi:10.1016/j.hlc.2007.06.012

Following obtaining informed consent, a follow upself-administered questionnaire was mailed to partici-pants. Information was collected relating to knowledgeof risk factors using the Indiana Cardiac RehabilitationKnowledge Questionnaire. Comparison between correctidentification of modifiable risk factors and the self-reportof personal risk factor of the participant was computedusing chi-square test.Results: Completed questionnaires were received from202 participants (75%). High cholesterol (87%), smoking(83%), hypertension (82%), were the most frequently iden-tified risk factors. Only a third of participants recognisedall seven modifiable risk factors. Of concern, 35% of theparticipants with documented diabetes did not recognisediabetes as a risk factor.Conclusions: Overall the results of this study provideevidence of the lack of knowledge of the modifiablerisk factors among patients post-PCI. The findings haveimportant implications for nursing practice in terms ofdirecting educational efforts at disseminating informationand engaging commitment to behaviour change.

doi:10.1016/j.hlc.2007.06.013