pcb 4233c immunology (80999) department of biological ...student research project because of a...

PCB 4233C Immunology (80999)

Department of Biological Sciences

College of Arts and Sciences

Instructor: Dr. Clifford M. Renk, PhD

Professor,

Department of Biological Sciences

Office: 222 Whitaker Hall

Phone: 590-7482

Email: [email protected]

Office Hours: M/W: 10:30AM-12:00Noon Other hours by appointment

Course Description

Fundamentals of immunology will be studied encompassing antigen-antibody structure and

reactions. Genetics, cellular immunity and immunopathology will be explored in an integrated

lecture/laboratory course

Credits: 3

Prerequisites: CHM 2211C and BSC 1010C

Meeting Times and Place Monday and Wednesday, 8:00AM – 10:15 AM

243 Whitaker Hall

Text The Immune System, 4th Ed. Peter Parham, Garland Publishing Co. New York, NY, 2015

The text was chosen to provide you a good introduction to a subject rich in language and content.

Faculty Comments: The opportunity for learning in this course is a shared commitment between faculty and student. An

enthusiastic learning environment enriches the gaining of knowledge. As your faculty, we will be

involved with you in your learning and practice by our availability to guide and assist you in gaining the

maximum value from your educational experiences. We look to you for inquiry, engagement and

commitment to actively participate in the educational process. We will be corresponding with you

through your student email account. It is very important for you to have your student email activated. If

you need assistance with this, please review the FGCU instructions for activating, forwarding, and getting

help with your email at http://mercury.fgcu.edu/StudentAccts/faq.asp

mailto:[email protected]

http://mercury.fgcu.edu/studentaccts/faq.asp

Course Policies:

• Attendance: Tardiness, leaving class early or failure to come to class minimizes your

opportunity to learn. If you are not in class at the time assignments are given or begin, you forfeit

the opportunity to take part in that assignment or laboratory and you forfeit any points/credit for

activities for the classes you miss.

• Readings: Read the text chapter and class notes before coming to class. Unannounced quizzes

may be given periodically during class time.

• Cell Phones: All cell phones are to be in the OFF position during class time. Students using

phones during class time will forfeit the 5 points for that day. If a cell phone is used or rings

during an exam or quiz the student will receive a zero for that exercise. • Laptops: You are welcome to bring your laptop to class to take notes, review lectures etc.

During class time communication via email or viewing non class related material is not allowed.

If you do so you forfeit the right to bring your laptop to class for the remainder of the semester.

• All work is due on dates specified by the instructor. Work may be turned in earlier than the

specified due date, but will not be accepted after the due date.

• Laboratories: Laboratory exercises will be handed out before each session. Unfortunately, due

to the time constraints, no make-up laboratories can be offered or extra credit activities.

• Exams will be given on the date scheduled only. Lab Quizzes will be announced a session

before it will be given and after the lab exercise.

• The instructor will evaluate unusual circumstances resulting in tardiness or class absence on a

case-by-case basis. The circumstances must be communicated to the instructor as soon as

possible.

• If you experience a catastrophic event in your life that precludes you from completing course

requirements, you may elect to petition for an incomplete in the course if you are in passing

standing of the course to date.

• All safety guidelines set forth will be strictly adhered to.

• Plagiarism is defined broadly as claiming the work of another as you own. To avoid plagiarism,

you must properly cite the author of the work. For an review on plagiarism see

http://education.Indiana.edu/~frick/plagiarism/item1.html

• All students are expected to demonstrate honesty in their academic pursuits. The university

policies regarding issues of honesty can be found in the FGCU Student Guidebook under the

Student Code of Conduct and Policies and Procedures sections. All students are expected to

study this document which outlines their responsibilities and consequences for violations of the

policy. The FGCU Student Guidebook is available online at

http://studentservices.fgcu.edu/judicialaffairs/new.html

• Florida Gulf Coast University, in accordance with the Americans with Disabilities Act and the

university’s guiding principles, will provide classroom and academic accommodations to students

with documented disabilities. If you need to request an accommodation in this class due to a

disability, or you suspect that your academic performance is affected by a disability, please see

me or contact the Office of Adaptive Services. The Office of Adaptive Services is located in the

Wellness Building. The phone number is 239-590-7956 or Video Phone (VP) 239-243-9453. In

addition to classroom and campus accommodations, individuals with disabilities are encouraged

http://education.indiana.edu/~frick/plagiarism/item1.html%2520

to create their personal emergency evacuation plan and FGCU is committed to providing

information on emergency notification procedures. You can find information on the emergency

exits and Areas of Rescue Assistance for each building, as well as other emergency preparedness

materials on the Environmental Health and Safety and University Police Department websites. If

you will need assistance in the event of an emergency due to a disability, please contact Adaptive

Services for available services and information.

• All students at Florida Gulf Coast University have a right to expect that the University will

reasonably accommodate their religious observances, practices, and beliefs. Students, upon prior

notification to their instructors, shall be excused from class or other scheduled academic activity

to observe a religious holy day of their faith. Students shall be permitted a reasonable amount of

time to make up the material or activities covered in their absence. Students shall not be penalized

due to absence from class or other scheduled academic activity because of religious observances.

Where practicable, major examinations, major assignments, and University ceremonies will not

be scheduled on a major religious holy day. A student who is to be excused from class for a

religious observance is not required to provide a second party certification of the reason for the

absence.

• The faculty reserves the right to modify the syllabus as needed to accommodate situations that

may arise during the sequence of this course.

Grading The grades for the course will be determined by a variety of activities including exams,

reports, quizzes and other activities. The distribution of the grades is as follows:

Exams: 500 points (Five @ 100 points each)

Quizzes: 50 Total points regardless of the number of quizzes given.

Lab Practice/Exercises: 50 points (includes reports and lab activities and practice). Lab

activities will be given out prior to the lab.

• Attendance: Attendance and participation in all sessions are necessary to a good learning

environment. An unexcused absence or tardiness of a lecture/laboratory session will

result in a deduction of 5 points from the total grade. Excused absences will be given

only with a valid excuse (written) and must be conveyed to the instructor the day of the

absence or the next class period. Appointments for interviews, sports etc., must be given

by email 1 week prior to the scheduled event. The instructor reserves the right to deny the

excused absence. One unexcused absence will be allowed over the course of the

semester. A sign-in sheet is provided in class. You are responsible to sign the sheet as

you enter the class. If you do not sign the sheet you will be marked tardy/absent and

receive a 5-point deduction for that day.

Presentation: 50 points. Topics must be decided and submitted to the instructor by

September 1st. Failure to pick a topic by that date will result in a deduction of 10 points.

If you fail to submit a topic by September 8th , one will be assigned to you by random

draw from a list of topics chosen by the instructor. No duplication of topics will be

allowed and will be assigned on a first come-first serve basis so get your topic in early. It

is advised to talk to the instructor about your topic in advance to see if it is acceptable.

AIDS related topics are not acceptable. Students must choose a topic outside their current

student research project because of a possible conflict of interest and duplication from

past presentations. The presentation should be an overview and ~10 minutes in length.

Keep it short and to the point. All students must post 3 questions directly to CANVAS by

11:55PM November 10th on the content of the paper (No true/false questions).

Questions submitted by the student may be incorporated into the presentations and will

be used on the 5th exam. Students will evaluate the presentations and assign a score. Fifty

percent of the presentation score will be derived from the student composite score. As a

courtesy to your fellow students you must be present for all the student presentations to

receive credit for the presentation. If you fail to attend one of the two days assigned for

presentations, then you will forfeit the 50 points. Tardiness is not acceptable and will

result in loss of points as well. (5 points/missed presentation). The order of presentations

will be assigned by random draw on the first day of the presentations.

Garland Learning Modules: 50 Points

The publisher has provided 28 learning modules for the 17 chapters in the course. These

are designed to review the material in the chapters and provide feedback on your

understanding of the material. Completion of each module is worth 2 points or 56 points

total enabling you to receive 6 extra credit points toward your grade. Each set of modules

must be completed by the date specified to receive full credit. You can receive ½ credit if

you complete the module after the due date but within 2 weeks of the due date.

The Enrollment Link: https://my.rocketmix.com/enrollcourse.aspx?courseid=2902

• Total: 700 points

The final letter grade will be determined as follows:

A = 627-700 points

B = 557-626 points

C = 487-556 points

D = 417-486 points

F = <417 points

Potential Paper/Presentation Topics (Not an Exhaustive List)

Pathogenesis

Superantigens

LPS and endotoxin shock

Immune evasion strategies of viruses: HIV, herpes simplex, Epstein-Barr, HHV-8

Immune evasion strategies of bacteria: Salmonella, Mycobacterium, Legionella, Listeria

Allergies/Asthma

Why the large increase in asthma?

Food intolerance

New allergy therapies

TH1 vs. TH2 response in diseases

https://my.rocketmix.com/enrollcourse.aspx?courseid=2902

Oral tolerization to antigens

Specific allergic reactions: peanuts, shellfish, poison ivy

Autoimmunity (there are others besides these!)

Diabetes mellitus

Systemic lupus erythematous

Hashimoto's thyroiditis (apoptosis as a mechanism of autoimmunity)

Rheumatoid arthritis

Crohn’s Disease

Celiac Disease

Neuroimmunology

Multiple sclerosis and experimental allergic encephalomyelitis as a model

Alzheimer’s disease and vaccine developments

Dementia in AIDS

Stress and the immune system

Cancer

Immunotherapy for the treatment of cancer: cell immunotherapy

Humanized monoclonal antibodies: Herceptin, anti-lymphoma antibody

How do tumors cells evade/defeat the immune system?

Bone marrow transplantation

Check point inhibitor therapies

Treatment//Vaccines

Anthrax vaccine

Mucosal immunity and vaccines

Effectiveness of vaccines

Vaccine development strategies/safety

viruses/bacteria as vectors for vaccine delivery

peptide vaccines

DNA vaccines

Why is it so hard to make effectives vaccines against parasites? (e. g. malaria)

Edible vaccines

Unknowns/risks of past and present smallpox immunizations

Transplantation

Xenografting: possibilities, problems, and ethics

Transplantation and infection: West Nile virus, Epstein-Barr virus, Hepatitis B/C

Immunosuppression: new ideas on preventing rejection

Bone marrow transplants: graft versus host disease (GVHD)

Immunodeficiency’s (there are others besides these)

Lack of B and/or T cells-

Severe combined immunodeficiency (SCID)

Omenn syndrome

Phagocytic defects- Chediak-Higashi syndrome

Lack of B cells/antibody –

X-linked agammaglobulinemia,

Wiscott-Aldrich syndrome

MHC deficiencies

X-linked SCID

Ataxia telangiectasia

Adenosine deaminase deficiency (ADA)

Complement deficiencies

Disease prevention

Obesity and the immune response

Normal microbiota as a microbiological barrier and stimulator of immune system

Do natural products/vitamins help the immune system?

What happens when people stop vaccinating their children?

Influence of probiotics on immunity

Effect of microbiome on the immune system

Aging

Relationship to the immune system function

Immunological memory

Immunological mechanisms:

Antimicrobial peptides: defensins

Nitric oxide (NO): antimicrobial effects

How did the immune system evolve?

M cells and the delivery of antigen from the GI tract to lymphoid tissue

Schedule of Activities

Date Session Topics Readings & Lab Exercises

Aug 21-23.

Course Overview

Historical Perspectives

Lymphoid System

Innate Immunity: Immediate

Chapter 1, pp 1-27

Chapter 2, pp. 29-79

Lab Exercises: Lab Safety and

Review of Techniques

Immunology Pretest*

CANVAS Assignment must

be completed by Jan 11th

Chapters 1&2 Learning

Modules Due: Sep 5

These sessions will address the following learning objectives:

• Identify significant developments in the elucidation of the immune system

• Describe branches/organs of the immune system.

• Compare and contrast innate and acquired immunity

• List the components of the innate immune system


Aug 28-30 Innate Immunity: Immediate and Induced

cont.

Chapters 2 and 3, pp. 29-79

Chapter 3 Learning Modules

Due: Sep 5


Sep. 4

Sep 6

Sep 11-13

No Class Labor Day

Exam 1

Antibody Structure and Generation of B-Cell

Diversity

• Basic Structure of Immunoglobulins

• Antigenic Determinants

• Immunoglobulin Classes

• Monoclonal Antibodies

• Immunoglobulin Genetics



Due: Sep 22


• Define how antibody structure contributes to antibody specificity and effector function

• List the five immunoglobulin classes and their biological function; Describe the

organization of the immunoglobulin genes and class switching


Sep 18-20

Antigen Recognition by T Lymphocytes

• T cell Diversity

• T cell receptor complex

Antigen Processing and Histocompatibility

Antigens

• MHC genes-organization and

distribution

• Regulation of MHC genes

• MHC genes and

Immunoresponsiveness



Due: Sep 22


• List the different types of T cell and their function

• Describe the T cell receptor complex

• Describe how T cells recognize antigens

• Differentiate between MHC Class I and Class II molecules

• Describe the function of MHC receptors

• Describe the process of self-restriction

Date Session Topics Readings & Assignments

Sep. 25-27 B cell Development

• B cell maturation

• B cell abnormalities

Chapter 6, pp 149-175

Chapter 6 Learning Module

Due: Sep 29


• List the receptors used to identify T and B cells

• Describe the use of a flow cytometer in the identification of lymphoid cells

• Describe the development of B cells

• Discuss the progression of immunoglobulin gene rearrangement with the developing B

cell

• Discuss the abnormalities in malignant B cells in relation to B cell development


Oct 2-4 Development of T cells

• Thymus Education

• Positive and Negative Selection

• T cell Maturation

• T cell Malignancies



Due: Sep 29


• List the significant T cell surface markers

• Correlate T cell receptor development with T cell maturation

• List the types of T cells

• Describe the differences between alpha/beta and gamma/delta T cells and their function

• Describe lymphocyte maturation in tissues


Oct 4 Exam 2


Oct 9-11

T cell Mediated Immunity

• T cell trafficking

• T cell signaling

• T cell Subsets

Immunity Mediated by B cells and

Antibodies

• B cell activation

• Antibody production

• T independent and T dependent

antigens

• Immunoglobulin Effector Functions



Due: Oct 20



Due: Oct 20


• Describe the interaction of T cell receptors and membrane antigens

• List the properties of the significant cytokines

• Describe the potential therapeutic uses of cytokines

• Discuss the mechanisms involved in T cell cytotoxicity

• List and describe the assays used to measure T cell function

• List the procedures for measuring complement activity

• Correlate complement levels and activities with clinical syndromes

• Compare T independent and T dependent antigens

• Compare and contrast the classical and alternate complement pathways

• Describe the regulation of the complement system

• Discuss the consequences of complement activation and deficiencies


Oct 16-18

Preventing Infections at Mucosal Surfaces

• Phagocytosis

• Secretory Ig’s

Immunological Memory and Vaccination

• Long Lived B and T Cells

• Primary and Secondary Responses

• Effectiveness of Vaccines

• Types of Vaccines



Due: Oct 20



Due: Oct 20


• List various components of the innate and adaptive immune responses

• Describe the various pathways involved in inflammation

• Define immunological memory

• Describe the differences between a primary and secondary immune response

• Compare various methods of vaccination listing strengths and weaknesses of each


Oct 23

Exam 3


Oct 25-

Nov 6

Failures of the Body's Defenses

• Evasion of the Immune System

Inherited and Acquired

Immunodefiencies

IgE-Mediated Immunity and Allergy

Overreactions of the Immune System

• Hypersensitivity Reactions

Disruption of Healthy Tissue by the Immune

Response



Due: Nov 10

Chapter 14. pp 401-431


Nov 10


• Discuss the mechanisms and consequences of Type I of hypersensitivity reactions

• Describe the principal mediators involved in hypersensitivity reactions

• Discuss the timing of the reactions of hypersensitivity reaction

• List detection methods for and treatment modalities for hypersensitivity reactions

• Discuss the different models of innate and adaptive immunity evolution

• Discuss the differences and similarities between NK cells and Gamma-Delta T cells


Nov 3

Nov 8

Nov 10

Last Day to Drop Without Acad. Penalty

Exam 4

Presentation Questions Due


Nov 13

Nov 15

Nov 20

Nov 22

Transplantation of Tissues and Organs

Disruption of Healthy Tissue by the Immune

Response

Cancer and the Immune System

Study Day: No Class



Due: Dec 1

Chapters 16, pp 473-507


Due: Dec 1



Due: Dec 1

This session will address the following learning objectives:

• Discuss the immunological basis of organ transplantation

• List mechanisms/treatments to prevent graft rejection

• Discuss the mechanisms and hypersensitivity reactions

• Describe the principal mediators involved in the types of hypersensitivity reactions

• Discuss the timing of the reactions of each type of hypersensitivity reaction

• Describe the different mechanisms involved in T and B cell tolerance and the role of

antigen in each.

• Discuss possible mechanisms involved in the development of autoimmune diseases

• Describe the differences between systemic and organ specific autoimmune diseases

List selected autoimmune diseases and their immunological origin

• List detection methods for and treatment modalities for hypersensitivity reactions and

autoimmune diseases.

• Discuss the concept of immunological surveillance and tumor development

• Discuss the immunological detection and treatment of various tumors

__________________________________________________________________________

___


NOV 27-

29 Presentations

Dec 6 Presentations/Review/Study Day

Dec 11 Exam 5

pcb 4233c immunology (80999) department of biological ...student research project because of a...

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