142 WEDNESDAY, SEPTEMBER 6

P3.17.05 HORMONAL CHANGES AND THEIR INFLUENCE ON LIPIDS METABOLISM IN PHYSIOLOGICAL PREGNANCY E. Romeiko, R. Smolarczyk, .I. Wojcicka-Jagodzinska, P. Pi&a&i, K. Czajkowski, .I. Teliga, A. Malinowska, 2”d Dept. OBIGYN, Warsaw Medical School, Warsaw, Poland.

Objective: The aim of the study was to investigate the influence of total estrogens, human placental lactogen, estradiol and prolactine on lipids’ metabolism in physiological pregnancy. Methods: One hundred thirty-six women in the third trimester (36.1e3.4 weeks) of the physiological pregnancy entered the study. The following serum parameters were measured: total lipids, total LDL fraction, total cholesterol, free cholesterol, phospholipids, triglicerides, HDL- cholesterol, LDL-cholesterol, total estrogens, human placental lactogen, estradiol, prolactine. Methods generally accepted in clinical biochemistry were used. The statistical analysis and correlation were performed. Results: The following results were obtained: total estrogens and human placental lactogen create an increased of serum concentrations of: total lipids (p<O.OOl), total LDL fraction (p<O.OOl), phospholipids (p<O.OOl) and triglicerides (p<O.OOl) while total cholesterol, free cholesterol, HDL-cholesterol and LDL-cholesterol remain unchanged. Prolactine lowers the serum concentration of LDL-cholesterol (p<O.Ol) and does not alter any other of the lipids. Estradiol does not influence lipids’ metabolism. Conclusions: The elevated concentrations of lipids in women with physiological pregnancy are related to an increase of total estrogens and human placental lactogen. Prolactin has little effect on lipids also does not alter estradiol levels. This may suggest that the increase of the lipids’ concentration in physiological pregnancy might be related mainly to estradiol activity.

P3.17.06 HORMONAL CHANGES AND THEIR INFLUENCE ON LIPIDS METABOLISM IN THE PREGNANCY COMPLICATED WITH PRIMARY HYPERTENSION E. Romeiko, R. Smolarczyk, .I. Wojcicka-Jagodzinska, P. Piekarski, K. Czajkowski, J. Teliga, A. Malinowska, 2”d Dept. OBIGYN, Warsaw Medical School, Warsaw, Poland.

Objectives: The aim of the study was to investigate the influence of total estrogens, human placental lactogen, estradiol and prolactine on lipids’ metabolism in the third trimester of the pregnancy complicated with primary hypertension. Methods: Twenty-eight pregnant women with primary hypertension (35.Oe3.8 weeks, blood pressure 163~11/100~5.9 mmHg) entered the study. The following serum parameters were measured: total lipids, total LDL fraction, total cholesterol, free cholesterol, phospholipids, triglicerides, HDL-cholesterol, LDL-cholesterol, total estrogens, human placental lactogen, estradiol, prolactine. Methods generally accepted in clinical biochemistry were used. The statistical analysis and correlation were performed. Results: The following results were obtained: total estrogens (1753*774 mmoV1, human placental lactogen 7841*1919 ngiml, estradiol 23,884*10,022 pgiml, prolactine 2OOe51 r&ml). The levels of total estrogens, human placental lactogen and estradiol did not significantly differ form those in normal pregnancies while prolactine was decreased (p<O.OOl). Positive correlation occurred between total lipids and total estrogens (p<O.Ol). Human placental lactogen and prolactine did not correlate with any of the lipids’ parameters whilst estradiol elevated concentration of triglicerides (p<O.O3). Conclusions: The elevated concentrations of lipids in women with pregnancy complicated with primary hypertension are not related to the hormonal changes. The only positive correlation is between estradiol and triglycerides. These observations are different form physiological pregnancy in which an increase in serum lipids’ concentration is strongly related to total estrogen and human placental lactogen. The elevated serum lipids’ concentration in pregnancy complicated with primary hypertension may be related to the other yet unknown mechanism.

P3.17.07 LIPIDS METABOLISM IN PREGNANCY COMPLICATED WITH PRIMARY HYPERTENSION E. Romeiko, R. Smolarczyk, J. Wojcicka-Jagodzinska, P. Piekarski, K. Czajkowski, J. Teliga, A. Malinowska, 2”d Dept. OBIGYN, Warsaw Medical School, Warsaw, Poland.

Objective: The aim of the study was to evaluate eight parameters of lipids’ metabolism in pregnant women with primary hypertension. Methods: Twenty-eight women with primary hypertension (the studied group) and 136 healthy women (the control group) entered the study. All women were in the third trimester of pregnancy and there was no significant difference in mean gestational age 35.Oe3.8 vs. 36.1e3.4 wks. Patients in the studied group presented blood pressure 163~11/100~5.9 mmHg. The following parameters in blood serum were measured: total lipids, total LDL, total cholesterol, free cholesterol, phospholipids, triglicerides, HDL-cholesterol, LDL-cholesterol. Generally accepted clinical biochemistry methods were used. Results: Women in the studied group versus control group showed following results: total lipids 777*195 vs. 692*122 mg%; ~~0.03, total LDL 562*207 vs. 465elOO mg%; p<O.Ol, total cholesterol 289*84 vs. 264*40 mg%; NS, free cholesterol 83*27ss. 77*14 mg%; NS, phospholipids 340*68 vs. 298*47 mg%; p<O.O02, triglicerides 363*161 vs. 232*73 mg%; p<O.OOOl, HDL-cholesterol69*15 vs.69*20 mg%; NS, LDL-cholesterol 160*71 vs. 150*38. Conclusions: Women in the third trimester of pregnancy complicated with primary hypertension present significant alteration in lipids’ metabolism manifesting in elevated serum concentrations of total lipids, triglicerides, phospholipids and total LDL. Primary hypertension does not change serum concentrations of total cholesterol, free cholesterol, HDL-cholesterol and LDL-cholesterol.

P3.17.08 PHYSIOLOGICAL SIGNIFICANCE OF HYPERPOLARIZATION- ACTIVATED INWARD CURRENTS IN SMOOTH MUSCLE CELLS FROM THE CIRCULAR LAYERS OF PREGNANT RAT MYOMETRIUM Y. Inoue (l), K. Okabe (2), H. Soeda (2), T. Kawarabayashi (1) (1) Dept. OBIGYN, Fukuoka University, Fukuoka, Japan. (2) Dept. Physiol., Fukuoka Dental College, Fukuoka, Japan.

Objectives: The aim of this study was to clarify the properties of hyperpolarization-activated current were investigated in pregnant rat uterus (17- 19 days gestation). Study Methods: The microelectrode and patch-clamp techniques were applied to enzymatically-isolated rat myometrial cell and isometric tension recording was performed using myometrial strips. Results: The resting membrane potentials were -58.4 mV and 48.5 mV in longitudinal and circular muscle cells, respectively. Application of hyperpolarizing current pulses produced a time-dependent anomalous inward rectification of membrane potential only in circular muscle cells. Under voltage-clamp conditions, inward currents (I 3 were activated by long hyperpolarizing pulses below 60 mV in circular but not in longitudinal muscle cells. Application of Cs’, but not Cs+, reduced the amplitude of I ,in a concentration-dependent manner (an IC,, of 0.15 nM). The reversal potential for I, was -26.2 mV and the slope conductance was 5 nS/pF. Changes in the [K’], and [Na’], shifted the reversal potential, and I, amplitude increased with excess [K’], and decreased with low [Na’], The steady-state activation of I, was well fitted by a Boltzmann equation with a half-activation potential of -84.3 mV and a slope factor of 9.6 mV. Time courses of activation and deactivation of the current strongly depended on membrane potential, and were well fitted by a single exponential function. In isometric tension recording, application of Cs’, to the circular muscles reduced the frequency, but not the amplitude of spontaneous contractions in a concentration-dependent manner. Conclusions: It is concluded that in pregnant rat uterus Ih channels are predominantly distributed in smooth muscle cells from the circular layer. Since 1,is activated at the resting membrane potential, it is likely that this current contributes to the maintenance of resting membrane potential and spontaneous activity in circular smooth muscle cells of late pregnant rats.