optogenetic stimulation and humidity logging for sleep research · 2015. 10. 30. · physical...
TRANSCRIPT
Optogenetic Stimulation and Humidity Logging for Sleep
Research
Devin Cornell, Kyle Gassel, Catherine Kinchen
Advisors: Dr. Mihail Cutitaru, Dr. Matthew Thimgan
Presentation Outline● Customer and Project Motivation● Humidity Logging Project● Light Stimulation Project● Live Demo● Q & A
Customer● Dr. Matthew Thimgan
○ Sleep research
■ Fly food supply affected
by low humidity
■ Current light flashing
setup is inaccurate and
uneven
Motivation● Optogenetics is used to study
the genetic nature of sleep
● Light stimulation induces
sleep in flies
● Determine if lifespan is
increased by more sleep
● The research requires a
controlled environment for
growing Drosophila flies
Value Proposition● Light Stimulation Project
○ Solution that can be used for future optogenetics research
○ Solution could be shared with other researchers in the field
● Humidity Project
○ Will give customer confidence that incubation is ideal for fly
development
○ Humidity information could be shared with other researchers to
provide value to the field
Overview - Humidity Logging● Measuring humidity inside
fly incubation test tubes to
help ensure their survival
Hardware Setup● Arduino Uno
● DHT22 Humidity Sensors using
custom digital protocol
● Sunfounder SD card reader
● DS3231 RTC
● Custom pull-up board
Physical Design● Sensors housed in 3D printed
environment
● Inserted directly into fly
incubation tubes
● Removable cap
Output Log● Samples every 0.5
second
● 6 humidity and
temperature sensors
● Stored in text file, opens
properly formatted in
Excel
● Outputs over serial for
debugging
Overview - Light Stimulation● Flashing LED’s at flies
and monitoring their
sleep patterns
● Trikinetics- popular
optogenetics
technology
Hardware Setup● 8 very bright LEDs
● Flashing at ~10Hz
● NMOS switch controlled
by npn on output of
RasPi
● Potentiometer to adjust
brightness
● Spacing for heat
dissipation
● Array draws ~17W
Software Architecture● Raspberry Pi with web server
controls light flashing
● Existing computer controls
RPi via web interface
● External hardware needed to
control LED power level
Live Demo
Q&A
Concordiam Design Group
References[1] Inagaki HK, Jung Y, Hoopfer ED, et al. Optogenetic control of Drosophila using a
red-shifted channelrhodopsin reveals experience-dependent influences on courtship.
Nat Methods. 2014;11(3):325-32.
[2]http://ar.neuro.fchampalimaud.org/