one gene one enzyme hypothesis
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In the next few lectures, the following questions will be Addressed:. One gene One enzyme hypothesis. One gene One enzyme hypothesis. In the next few lectures, the following questions will be Addressed: What is the structure of a gene? How does a gene function? - PowerPoint PPT PresentationTRANSCRIPT
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One gene One enzyme hypothesis
In the next few lectures, the following questions will be Addressed:
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One gene One enzyme hypothesis
In the next few lectures, the following questions will be Addressed:
What is the structure of a gene?
How does a gene function?
How is information stored on the gene?
What is the relationship between genotype and phenotype?
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PathwaysBiologists and clinicians want to address the question of how altering a particular set of base pairs that make up the 3 billion base pairs in the human genome led to this phenotype.
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Alkaptonuria
Degenerative disease. Darkening of connective tissue, arthritisDarkening of urine
1902 Garrod characterized the disorder- using Mendels rules- Autosomal recessive. Affected individuals had normal parents and normal offspring.
1908 Garrod termed the defect- inborn error of metabolismHomogentisic acid is secreted in urine of these patients.This is an aromatic compound and so Garrod suggested that it was an intermediate that was accumulating in mutant individualsand was caused by lack of enzyme that splits aromatic rings of amino acids.
1958 La Du showed that accumulation of homogentistic acidis due to absence of enzyme in liver extracts
1994 Seidman mapped gene to chromosome 3 in human
1996 Gene cloned and mutant identified P230S &V300G
2000 Enzyme principally expressed in liver and kidneys
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How does a gene generate a phenotype?
The experiments of Beadle and Tatum in the 1940’s provided the first insight into gene function.
They developed the one gene/one enzyme hypothesis
This hypothesis has three tenets:
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Consequences of mutations
Lets say we know the biochemical pathway.
With this pathway, what are the consequences of a mutation in geneB?
Would the final product be produced?
Would intermediate2 be produced?
Would intermediate1 be produced?
What happens if we add intermediate1 to the media?
What happens if we add intermediate2 to the media?
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Neurospora
Beadle and Tatum analyzed biosynthetic mutations in the haploid fungus Neurospora.
It had the advantage in that it could be grown on a defined growth medium.
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Arginine biosynthetic mutants
Beadle and Tatum set out to identify genes involved in the biosynthetic pathway that led to the production of the amino acid arginine.
Neurospora has approximately 15,000 genes and only 4-5 of these genes are involved in synthesizing arginine.
How do you identify five genes from 15,000?
The POWER OF GENETICS!!!!!!
Typically the organism is exposed to a strong mutagen.
This randomly mutagenizes genes.
Then you look for a mutant in the pathway of interest.
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The method
1 2 3 4 5 6 7 8 9 10
complete
minimal
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Conclusion-strain1
Strain1 and 7 are defective in either amino acid production or Vitamin production
Complete media(salt+sugar+
Vitamin + amino acids)
Minimal media(salt+sugar)
+ 20 amino acids
Minimal media(salt+sugar)+ vitamins
Minimal media(salt+sugar)
Conclusion:
Complete media(salt+sugar)
Vitamin + amino acids
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Conclusion- strain7
Strain1 and 7 are defective in either amino acid production or Vitamin production
Complete media(salt+sugar+
Vitamin + amino acids)
Minimal media(salt+sugar)
+ 20 amino acids
Minimal media(salt+sugar)+ vitamins
Minimal media(salt+sugar)
Conclusion:
complete media(salt+sugar)
Vitamin + amino acids
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Which amino acid?
Beadle and Tatum found that three mutants could not produce arginine
Arg1 Arg2 Arg3
The biochemical pathway for arginine synthesis was kind of known. Ornithine and citrulline are closely related to arginine and were thought to be precursors
Instead of arginine, if they added ornithine or citrulline to the media, some mutants were rescued and others were not
Precursor -----> ornithine -----> citrulline -----> arginine
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Add back
Instead of arginine, if they added ornithine or citrulline to the media, some mutants were rescued and others were not
Ornithine CitrullineArginine
Mutant1
Mutant2
Mutant3
Precursor -----> ornithine -----> citrulline -----> arginine
enz1 enz2 enz3
There are three different enzymes required for arginine synthesis
Enz1, enz2 and enz3
Beadle and Tatum isolated three different mutations in genes (three genes)
Arg1 Arg2 Arg3
?????Which mutation codes for which enzyme????
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Mutant in Arg1- only precursor made
Add ornithine or citrulline to media, downstream enzymes are functional and pathway continues---> arginine synthesized
Mutant in Arg2- You need to supplement media with citrulline for the pathway to continue. Adding the precursor or ornithine does not help.
Mutant in Arg3-You need to supplement media with arginine. Adding the precursor, ornithine or citrulline does not help.
These experiments demonstrated that a single gene (mutation) coded for a single enzyme.
In addition, the combination of appropriate mutations and intermediates enabled Beadle and Tatum to define the biochemical pathway leading to Arginine synthesis.
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This rationale currently is being used in many laboratories to elucidate more complex pathways in multicellular organisms
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Review
Solving biochemical pathways:
The more mutations that a compound rescues, the later in the pathway the compound is located
Conversely, the later a mutation is in a pathway, the fewer compounds will rescue it:
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Another example
CompoundE B N A
Mut1
Mut2
Mut3
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What is the order of the compounds and mutations in the pathway?
The steps in a biochemical pathway identified by this procedure are dependent on the available intermediates and mutations.
This procedure does not identify every step in the pathway
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Biosynthetic pathways at the grocery store
Most of the red and blue colors found in higher plants are a result of pigments synthesized from one of two metabolic pathways, the carotenoid or the anthocyanin pathway.
Grocery store corn is usually yellow. Which step in the pathway must be mutated to produce yellow corn?
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The Flavor-Saver Tomato
Ethylene gas released by fruit accelerates the ripening process
Prevention of ethylene production would block the fruit from ripening prematurely and spoiling on the way to the market.
The ethylene biosynthetic pathway is as follows:
Precursor-----> ACC------> ethylene
Which step in the pathway must be mutated to prevent Ethylene production?
ACCsynthase
ACCoxidase
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Temperature-sensitive mutations
The one gene/one enzyme concept explains a number of genetic phenomena
A) Temperature-sensitive mutations
Some mutations exhibit a phenotype at high temperatures (the restrictive temperature) but function normally at lower temperatures (permissive temperature). The mutation results in a slight destabilization and alteration of the 3D conformation of the enzyme
An example of a TS mutation:
Dogs and cats that are white with black feet or vice versa
The gene for coat color is normal at cold temperatures
The extremities are colder than the body, therefore the enzyme is active in the feet and produces color.
Low temp- structure of enzyme- normal- activity normal
High temp- structure of enzyme-altered- No activity
These kinds of conditional mutants allow you to turn on and off a function of a protein.
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Dominance versus Recessive
The one gene/one enzyme concept explains a number of genetic phenomena
B) Dominance versus Recessive
Genotype enzyme activityphenotype
A/A 2X normal
A/a 1X normal
a/a 0X mutant
Usually substrate is limiting, enzyme is in excess
By saying that a mutation is recessive, we are saying that
1 unit (or 50% of the normal activity) is enough to produce
a normal phenotype
Precursor------> product------> phenotype
enzymeA
geneA
^|
^|
キ A allele produces functional enzyme
キ a allele produces nonfunctional enzyme
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Genetic RatiosThe one gene/one enzyme concept explains a number of genetic phenomena
C) Genetic Ratios
The one gene/one enzyme hypothesis also explains phenotype ratios observed in a standard dihybrid cross:
Precursor----> intermediate----> productyellow white blue
EnzA EnzB
Parental cross white x yellow
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AB Ab aB ab
AB
Ab
aB
ab
9 A-B- blue3A-bb white3aaB- yellow1aabb yellow
Precursor----> intermediate----> productyellow white blue
EnzA EnzB
F2
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Labradors
Parental Cross: black x yellow
BBEE bbee
BbEe (black)
x
BbEe (black)
Given the pathway show above, what phenotypic ratios would be
produced in progeny from the dihybrid cross:
BbEe x BbEe
Yellow-------> brown--------> blackE B
EB Eb eB eb
EB
Eb
eB
eb
9:3:4
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Precursor----> intermediate----> productwhite white blue
EnzA EnzB
AB Ab aB ab
AB
Ab
aB
ab
9 A-B- blue3A-bb white3aaB- white1aabb white
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Biochemical Pathways and Linked Genes
The following F2 progeny are produced:
50 yellow 40 blue 10 white
Precursor----> intermediate----> productyellow white blue
EnzC EnzD
GeneC GeneD
Parental C-D x c-dC-D c-d
F1
What is the map distance between these two genes?
Map Distance+#Recombinants/Total Progeny x 100%
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One gene: one polypeptide
The concept of 1 gene/enzyme was modified to the concept of: 1 gene/ 1 protein
Almost all enzymes are proteins but not all proteins are enzymes. Many proteins provide structural rather than enzymatic roles.
For example polymers of the protein actin provide structural integrity to the eukaryotic cell.
Perhaps the most notable example of this comes from studies of Hemoglobin.
Hemoglobin is an iron carrying protein found in the red blood cells and is responsible for transporting oxygen from the lungs to the cells of the body.
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Hb
Hemoglobin consists of four polypeptides (proteins) each associated with a specific Heme group (Heme is a small iron containing molecule to which oxygen can attach) Adults contain 2 alpha polypeptides and 2 beta polypeptides
Alpha polypeptide = 141 amino acids
Beta polypeptide= 146 amino acids
Over 300 known hemoglobin variants are known and each is the result of a specific mutation
Most of these are the result of a single amino acid substitution
キ Hb A:
キ Hb S:
キ Hb C:
These results demonstrate that:
1. Genes specify proteins that are not enzymes
2. Mutations can disrupt a single amino acid out of the many that make up the protein.