NOVA PHARMA NEWS LETTER

Published by

Nova College of Pharmaceutical Education and Research

(Nova Educational Society)

Jupudi, Ibrahimpatnam, 521456

VISION:

To sTrive for and creaTe a “cenTre of excellence” in The field of

Education and Research involving wide areas of Pharmaceutical field.

Content Institution Profile

Infrastructure and Academics

List of Publications/Presentation

Research/ Awards/ Fellowships

Industry-Institution Interaction

List of Seminar and workshops organized

Research Note

Editor: Dr. P. Selvam., M.Pharm Ph.D.,FNABS.,

Professor and Principal, Nova College of

Pharmaceutical Education and Research(NCPER)

Jupudi, Ibrahimpatnam, 521456. Krishna DT, A.P

E-Mail Id-Pharmacyibm2007@gmail.com.

Volume 1, Issue 6, 2014

Volume 1, Issue 6, 2014

Secretary

President

Volume 1, Issue 6, 2014

Institution Profile: Nova College of Pharmaceutical Education and Research was established in the academic year

2007-2008 in green serene, pollution free, calm area called Jupudi. Our college is affiliated to JNTU

Kakinada and is recognized by AICTE and PCI, New Delhi. The college started with an intake of 60

students and there were a total of 58 outgoing students in the first batch with a total pass percentage

of 98% in B. Pharmacy course. Post graduation in Pharmacy was started in October 2011, and

presently the college is offering Bachelor of Pharmacy and Masters of Pharmacy in Pharmaceutics,

Pharmacology and Pharmaceutical Analysis with a total strength of 400 students.

INFRASTRUCTURE:

The institution is well equipped with modern, advanced and sophisticated instruments and apparatus in order

to offer the training and research need of the students as per norms of the Pharmacy council of india, AICTE

and JNTU-K. The machine room and M. Pharmacy research laboratories, in particular are well facilitated with

state of the art equipment to serve the need of post graduate students of Pharmaceutics and Pharmacology.

The library is spacious and sufficiently stocked with huge collection of books and journals. The library is

computerized and has about 6000 text books needed for B. Pharmacy, M. Pharmacy courses. In addition to

this the library is well furnished with various national, international journals of Pharmacy, Science and

technology and online library facilities for students and staff to access hundred of research information.

ACADEMICS:

Three batches from B.Pharmacy were released with a good result of 95% and 85% of the final year students

secured good result in 2012-13 & 2013-2014, respectively. Many of our outgoing students are placed in

reputed industries, colleges and few are placed in pharmaceutical organizations in USA and UK. Our college

is continuously busy with various activities like seminars, sports, industrial tours and educational activity.

Volume 1, Issue 6, 2014

Principal Profile

Name of Teaching Staff

Dr. P. Selvam

Designation Principal & Professor

Department Pharmacy

Date of Joining the Institute 03-01-2013

Qualification with Class/Grade

UG

B. Pharm

(1995)

PG

M.Pharm

(Pharm.

Chemistry)

(1999)

Ph.D

(Pharma. Sciences)

TN Dr M.G.R Medical

University, Chennai

(2009)

Total Experience in Years

Teaching

15 years

Industry

------

Research

10 Years

Papers Published

National

50

International

25

Papers Presented in Conferences

National

50

International

40

PhD Guide? Give field & University

Field

Pharmaceutical Sciences

University

JNTU Hyderabad,

Savitha University

Chennai

PhDs/ Projects Guided

PhDs

02

Projects at Masters level

20

Books Published IPRs/Patents : Phytochemical Investigation and Anti-HIV activity of

Morinda citrifolia published by WNRF, Chennai,

US Patent 13/283281 dated 27th

October 2011

Professional Memberships : APTI, ISAR,ISNS, NMRS,NABS,IAMPV

Consultancy activities : Noni Biotech Pvt. Ltd Chennai (Rs 11,00,000)

Awards : DST BOYCAST Fellowship 2007,

Noni Outstanding Scientist Award 2011

Fellow of National Academy of Biological Sciences

Noni Service Excellence Award 2013

Grants fetched : Rs. 10 lkhs From Noni Biotech Pvt Ltd Chennai

Interaction with Professional Institutions : National Institute of Immunology, New Delhi, IIT Madras

Volume 1, Issue 6, 2014

List of Publications/Presentation

M. Chandramohan Periyasamy Selvam, Priya Srinivasan, Tanvi Khot, R. Padmanaban,

“Studies of Dengue NS3 Protease Inhibitory Activity of Novel Isatin Derivatives”

International Journal of Pharmaceutical Innovations, 2(3), 21-36, 2014

Periyasamy Selvam,. Guoping Hu, Yun Tang, Xi Li, Jin Huang. “

LEDGF- HIV Integrase

inhibitory activity of 2-methylpyrazol-5-one and 2-amino-4-phenyl-thiazole” International

Journal of Pharmaceutical Innovations, 2(3), 37-46, 2014

Balu Chennama , Periyasamy Selvam , Erik De Clercq., Christophe Pannecouque. Synthesis,

“Antiviral and Cytotoxic Activities of Novel 2, 3-Disubstituted Quinazolin-4(3H)-ones”,

International Journal of Pharmaceutical Innovations, 2(3), 47-59, 2014

Atukuri.S.N.Malleswari, Periyasamy Selvam., “Investigation of Hepatoprotective activity of

morinda citrifolia Juice and Garcinia cambogia Mix.,” International Journal of Pharmaceutical

Innovations, 2(3), 7-20, 2014

Volume 1, Issue 6, 2014

Research/ Awards/ Fellowships:

Dr. P. Selvam invited as Screening committee member for selection of research papers-

National Seminar on “SIPRA INOVATIVE PHARMA RESEARCH AWARD 2014”

organized by SIPRA Labs Hyderabad 2-3, July 2014

Anticancer potential (breast cancer) of Siddha formulation (BH) were identified in

collaboration with Roever Drugs , Tamil

Volume 1, Issue 6, 2014

Volume 1, Issue 6, 2014

INDUSTRIAL -INSTITUTION INTERACTION:

1. Noni Biotech Pvt. Ltd., Chennai made a collaboration with our institution for the

“Study and Safety assessment of Noni Fruit Juice concentrate”.

2 . Nova College of Pharmaceutical Education and Research enter research

collaboration with OyaGeninc, Rochester, New York, USA for Designing of Novel activator of APOBEC-.3G as potential inhibitor of HIV replication.

3. Nova College of Pharmaceutical Education and Research made research collaboration

with Makore Labs Hyderabad for small molecule drug discovery

4. NCPER working with laboratory of Molecular Virology, Dept of Biotechnology, IIT-M

for investigation of anticancer activity of Noni Juice in association with Noni Biotech,

5. NCPER working with Biotechnology, Manipal University for investigation of

hepatotoxicity and hepatoprotective activity of Noni Juice in association with Noni

Biotech, Chennai

6. NCPER working SPIRA Laboratory Hyderabad for investigation of Preclinical Safety

activity of Noni Juice in association with Noni Biotech, Chennai

7. NCPER working with VPCRI Delhi University for investigation of antiviral activity

against Influenza virus

Volume 1, Issue 6, 2014

List of Seminar and workshops organized:

1. Nova College of Pharmaceutical Education & Research, organized I.B.Pharm Students

Poster Presentation on “HUMAN ANATOMY AND PHYSIOLOGY” on 12.7.2014

2. Seminar on “Molecular modeling and Drug Design” given by Mr.V. Ashok Kumar

B.Pharm., M.S (Med. Chem)., NIPER, Hyderabad on 12.7.2014

3. Seminar on “Resealed Erythrocytes Drug Delivery System” Given by

Dr. B. Krishnamoorthy., M. Pharm., Ph.D., Professor and Principal, Montessori

Siva Sivani College of Pharmacy Krishna Dt., A.P on 19.7.2014

4. Seminar on “A Comparative study for the assessment of quality of Life using MDI &

CSII for Type-1 Diabetes patients” Given by N. Suresh., M. Pharm (Pharm. Practice) on

18.5.2014

Volume 1, Issue 6, 2014 9

Presentation of Research papers in National Seminar on “SIPRA

INOVATIVE PHRAMA RESEARCH AWARD 2014 , ORGANISED BY

SIPRA LABS, HYDERABAD July 2-3, 2014.Research papers presented by

P.G and Ph.D students of NCPER

Volume 1, Issue 6, 2014 10

Nova College of Pharmaceutical Education & Research , organized I.B.PHARM STUDENTS POSTER PRESENTATION ON “HUMAN ANATOMY AND PHYSIOLOGY” on 12.7.2014

Volume 1, Issue 6, 2014

Volume 1, Issue 6, 2014 11

SEMINAR ON “MOLECULAR MODELLING AND DRUG DESIGN” DELIVERED BY Mr. V. Ashok Kumar B. Pharm., M.S (MED. CHEM), NIPER, HYDERABAD ON 12.7.2014

Volume 1, Issue 6, 2014 12 SEMINAR ON “RESEALED ERYTHROCYTES DRUG DELIVERY SYSTEM” GIVEN BY Dr.B.KRISHNAMOORTHY., M.PHARM., PH.D. PROFESSOR AND PRINCIPAL, MONTESSORI SIVA SIVANI COLLEGE OF PHARMACY KRISHNA DT., A.P ON 19.7.2014

Volume 1, Issue 6, 2014, 13

Seminar on “A Comparative study for the assessment of quality of Life using MDI & CSII for Type-1 Diabetes patients” Given by Mr. N. Suresh., M.Pharm (Pharm Practice) on 18.5.2014

Volume 1, Issue 6, 2014

Volume 1, Issue 6, 2014 14

Research Note

HIV RNase H inhibitory activity of Novel Isatine-Sulphadimidine derivative

Dr. P. Selvam, Nova College of Pharm. Education and Research, Jupudi, Krishna Dt .A.P

Dr. C. Suresh Kumar, Dr. B. Babajan, Dept of Biochemistry, S. K. University, Anantapur

Introduction:

HIV RNase H important enzyme for HIV replication and validated target for Designing potential

anti-HIV agents. We identified isatin derivative (SPIII-5H) as potential inhibitors of HIV RNase

H (IC50: 8.4 µM). Our objective is to study a molecular modeling of SPIII-5H with HIV RNase H

to understand the mechanism.

Methodology:

The AutoDock (Version 4.0) [1] program was chosen to dock the SPIII-5H into HIV RT explore the binding

mode between the SPIII-5H and the receptor. The 3D coordinates of HIV RT complex with ligand were taken

from the Brookhaven Protein Databank (PDB code: 2I5J) [2]. AutoDock tools (ADT) were used to prepare the

ligand, protein (deleting all water molecules, adding polar hydrogens and loading Kollman United Atoms

charges) and also to perform docking calculations. A grid box with the dimensions60 X 50 X 50 points was

constructed around the binding site, based on the location of the co-crystallized ligand. All bond rotations and

torsions for the ligand were automatically set in the ADT. Considering the flexibility of the protein during the

ligand–receptor interaction process, the active site residues were defined as flexible residues in the docking

process. The Lamarckian genetic algorithm (LGA) procedure was employed and the docking runs were set to

50. The rest of the parameters were taken as default.

Results and Discussion

The computer simulated automated docking studies were performed using the widely distributed

molecular docking software, AutoDock 4.0. Energy minimized SPIII-5H from Dundee PRODRG2 server

[3], were docked with HIV RT. The SPIII-5H specifically binds to HIV RT of RNASe H region amino

acids Thr296, Ala261, Ser265, Asp498 and Ala538 (Figure 1). Results were obtained under 298.15 K

temperatures with solvation of water molecule as the solvent parameter. AutoDock binding affinities of

the SPIII-5H, evaluated by free energies (∆Gb, kcal/ mol) inhibition constants (Ki), hydrogen bonds, and

RMSD values. The obtained success rates of AutoDock is highly excellent, where the docked SPIII-5H

binding free energies -10.42 kcal/mol at 0.02 Ao RMSD (Tabel 1) and predicted hydrogen bond forms

between SPIII-5H and MEKK1 of Thr296, Ala261, Ser265, Asp498 and Ala538 (Figure 1)

Table 1: Docking energies of SPIII-5H with HIV-1 RT

Protein Inhibitors Clustera RMSDb Lowest Binding Energyc

(Kcal/mol)

Inhibition Constant

d (Ki)

Amino acids involved in H

bond formation

HIV-1 RT SPIII-5H 05 0.02 -10.42 21.879n

M

Thr296, Ala261,

Ser265, Asp498

and Ala538 aIndicative of the total number of binding modes produced

bHeavy atoms root-mean-square deviation with respect to the experimental structure. cThe change in binding free energy is related to the inhibition constant using the equation: ∆G = RT in Ki,

whereR is the gas constant 1.987 cal K-1 mol-1, and T is the absolute temperature assumed to be 298.15 K. dEstimated inhibition constant at 298.15 K.

Effect of SPIII-5H compounds on HIV-1 RNase H activity

Compound

Structure HIV-1 RNase-H

aIC50

SPIII-SH

8.4

aCompound concentration required to reduce the HIV-1 RT associated Ribonuclease H (RNaseH) activity by 50%.

Fig.1: Binding mode of SPIII-5H with HIV-1 RT at RNase H region.

Volume 1, Issue 6, 2014 16

References:

Babajan, B., Chaitanya, M., Rajsekhar, C., Gowsia, D., Madhusudhana, P.,

Naveen, M., Chitta, S.K., Anuradha, C.M. (2011) Comprehensive structural and

functional characterization of Mycobacterium tuberculosis UDP-NAG enolpyruvyl

transferase (Mtb-MurA) and prediction of its accurate binding affinities with

inhibitors. Interdiscip Sci.:3:204-16

Himmel DM, Sarafianos SG, Dharmasena S, Hossain MM, McCoy-Simandle K, Ilina

T,Clark AD Jr, Knight JL, Julias JG, Clark PK, Krogh-Jespersen K, Levy RM, Hughes

SH, Parniak MA, Arnold E. HIV-1 reverse transcriptase structure with RNase H inhibitor

dihydroxy benzoyl naphthyl hydrazone bound at a novel site. ACS Chem Biol. 2006 Dec

20;1(11):702-12.

Schuettelkopf, A.W., van Aalten, D.M.F. (2004) PRODRG- a tool for high-

throughput crystallography of protein-ligand complexes. Acta Crystal, :60:1355 -1363.

NOVA COLLEGE OF PHARMACEUTICAL EDUCATION AND RESEARCH

JUPUDI, IBRAHIMPATNAM, KRISHNA DT, A.P

(APPROVED BY PCI AND AICTE, NEW DELHI AND AFFILIATED TO JNTU-K)

COURSE OFFERED

B.PHARMACY 100 SEATS

M.PHARMACY

PHARMACEUTICS

PHARMACOLOGY

PHARMACEUTICAL ANALYSIS AND QUALITY ASSURENCE

Volume 1, Issue 6, 2014

Our Group of Institutions

Vijayawada & Ibrahimpatnam

Nova College of Engineering and Technology (Co-Ed)

Nova College of Pharmaceutical Education & Research

Nova College of Education (B.Ed. & M.Ed.)

Nova P.G College (M.B.A)

Nova P.G College (M.C.A) Nova D.Ed. College

Hyderabad & Jafferguda

Nova College of of Engineering and Technology

Nova P.G College (M.B.A)

Nova College of pharmaceutical Education & Research

Nova Business School

Narayana Engineering & Technical Campus

Priyadarshini Degree & PG College

Priyadarshini College of Business Management Priyadarshini College of Computer Science & Research

Jangareddigudem

Nova College of Engineering and Technology

Nova College of Pharmacy

Nova College of Education (B.Ed. & M.Ed.)

Nova College of Physical Education (B.P.Ed.) MVN Memorial College of Education

Elluru

Nova’s Institute of Technology MVN Institute of Management Studies & Research

Nuzvid

Nova College of Business Management

Nova College of Elementary Education (D.Ed.) Volume 1, Issue 6, 2014