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Bio Research

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BioResearch

NeurobiologyResearch Tools for Biologically Relevant Results

Neurobiology Research Tools

BioResearchNeurobiology Research Tools

Avoid Research Roadblocks and Take a Direct Path to Results

Primary Cells and Media

Transfection

" Non-viral transfection doesn’t give me the transfer efficiency I’m looking for."

" Sourcing cells from specific species and brain regions requires a lot of my time and effort."

SOLUTION: Select from Lonza’s range of well-characterized and ready-to-use primary cells. Simply thaw and culture. Page 5

SOLUTION: Our non-viral Nucleofector™ Technology delivers 70% gene transfer efficiency and high expression levels. Page 9

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Cell Analysis

" I can’t find reliable, ready-to-use assay tools for mRNA, protein expression, cell proliferation, or cytotoxicity."

SOLUTION: Choose from our variety of neurology-specific assay tools, including qPCR arrays, ready-to-use protein gels, and bioassay kits to measure cell proliferation andcytotoxicity. Page 14

Drug Discovery

" I need human model systems to avoid species extrapolation in my drug discovery process."

SOLUTION: Now you can access assay-ready embryonic stem cell-derived pure human motor neurons. Page 13

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Your Complete Neurobiology Tool Kit

Streamline your neurobiology workflow by choosing from convenient, innovative research tools that have been designed and tested to work together.

Lonza solutions provide biologically relevant results, from high-quality primary cells, through efficient transfection technology, to expert analy-sis tools.

Primary Cells and Media Transfection Cell Analysis Drug Discovery

Ready-to-use cryopreserved Clonetics™ or Poietics™ Primary Neural Cells:– Astrocytes

(mouse, rat, or human)– Cerebellar granule cells (rat)– Cortical neurons (mouse or rat)– Dorsal root ganglia

(rat; embryonic or neonatal)– Hippocampal neurons

(rat or mouse)– Hypothalamus neurons (rat)– Neural progenitor cells (human)– Retinal neurons (rat)– Striatum neurons (mouse or rat)

Optimized Clonetics™ Primary Neural Cell Growth Media:– PNGM™ or PNGM™-A BulletKit™

Media for embryonal or adult primary animal neurons

– AGM™ BulletKit™ for human and animal astrocytes

– NPMM™ or NPDM™ BulletKit™ Media for neural progenitor cell growth or differentiation

Amaxa™ Nucleofector™ Technology with optimized protocols for primary cells, including:– Hippocampal neurons– Cortical neurons– Dorsal root ganglia– Cerebellar granule cells– Astrocytes– Oligodendrocytes– Neural stem cells

Also optimized for numerous cell lines, including:– PC-12– SH-SY5Y– Neuro2A

StellARray™ qPCR Arrays for mRNA expression profiling:– Alzheimer's (mouse or human)– Autism (mouse or human)– Huntington's (mouse or human)– Mood disorder

(mouse or human)– Neurodegeneration

(mouse or human)– Parkinson's (mouse or human)

Protein Expression Analysis:– PAGEr™ Precast Protein Gels for

Western blotting

Assay-ready human embryonic stem cell (ESC) derived neurons: – NeuroPlate™ Human ESC

Neuronal Progenitors– MotorPlate™ Standard Human

ESC Motor Neuron Progenitors– MotorPlate™ Mature Human ESC

Motor Neuron Progenitors

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Primary Neural Cells and Growth Media

Rat Mouse(CD1 or C57)

Recommended Media

Hippocampal neurons n n PNGM™

Cortical neurons n n PNGM™

Striatum neurons n n PNGM™

Dorsal root ganglia (embryonic or neonatal)

n

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PNGM™

Cerebellar granule cells n – PNGM™-A

Hypothalamus neurons n – PNGM™

Retinal neurons n – PNGM™

Rat cortical neuronal cells were thawed, cultured 14 days, and immunofluores-cently stained with anti-PGP 9.5 and anti ß-tubulin.

Access neural cells when you need them. Choose from Lonza’s extensive range of isolated primary neural cells and culture them in media that we have already optimized and tested to give you the best results. You will never have to put research on hold for animal pregnancies again. Simply store Lonza cells in your freezer and thaw them as needed.

Cryopreserved Clonetics™ Primary Neurons

Choose from our broad selection of high-quality, high-yield cultures of dissociated primary animal neurons that are:

– Tested for neuron-specific markers – Guaranteed to perform in culture – Guaranteed free of mycoplasma and bacteria – Tested to ensure they will perform after shipping – Lots can be reserved to repeat experiments using cells from the

same batch

Clonetics™ PNGM™ Primary Neuron Growth Media

Clonetics™ PNGM™ gives you a proven alternative to Neurobasal™ and B27® for long-term culture of embryonic or adult rat and mouse neurons:

– Serum-free media in BulletKit™ Format – Guaranteed to perform with all Clonetics™ Primary Animal Neurons – Batch tested with primary neurons and a pre-screened B27®-

equivalent supplement

Applications – Culture embryonic primary neurons in PNGM™ BulletKit™ Media. – Culture adult primary neurons and cerebellar granule cells in

PNGM™-A BulletKit™ Media

Clonetics™ Rat Cortical Neurons were seeded at 40% recommended density and cultured in PNGM™ BulletKit™ Media and a competitor media. After 6 DIV, viability was determined by ViaLight™ Plus Assay. Values were normalized to “no cell” control.

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Growth of Rat Cortical Neurons in Different Media

Applications – Transfection – Evaluating electrophysiological properties, neurotransmitters,

and receptor function – Drug screening – Research into ion channels, intracellular transport,

and neurotoxicity

" Sourcing cells from specific species and brain regions requires a lot of my time and effort."

SOLUTION: Select from Lonza’s range of well-characterized and ready-to-use primary cells. Simply thaw and culture.

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Cryopreserved Clonetics™ Primary Astrocytes

Improve your workflow with cryopreserved, high-quality human, and animal glial cells. There’s no need for animal care or glial cell isolation with these products, which are:

– Passaged once after isolation – Batch tested for growth characteristics and morphology (GFAP) – Guaranteed free of mycoplasma – Able to be reserved by lot so you can repeat experiments using cells

from the same batch

Rat Mouse(CD1 or C57)

Human

Hippocampal astrocytes n – –

Cortical astrocytes n – –

Striatum astrocytes n – –

Cx-Hi-Cp mixed Astrocytes n – –

Cx-Hi-Striatum mixed Astrocytes – n –

Astrocytes – – n

Indirect immunofluorescence staining of Clonetics™ Normal Human Astrocytes for glial fibrillary acidic protein (GFAP).

Applications – Transfection – Pharmacology – Drug screening – Research into neurogenesis, cell physiology, Alzheimer’s disease,

Parkinson’s disease, spinal cord injury, astrocyte-mediated neuro-toxicity, ion channels, electrophysiology, and patch clamping


Clonetics™ AGM™ Primary Astrocyte Growth Media

Culture human and animal astrocytes with serum-free media, in BulletKit™ format. This media has been tested and guaranteed to perform with all Clonetics™ Primary Astrocytes.

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Normal human neural progenitor cells stained for ß-tubulin III and GFAP.

Cryopreserved Poietics™ Neural Progenitor Cells

Safeguard your experiment with progenitor cells that are guaranteed to differentiate into a mixed population of neuronal cells that express stipulated markers on laminin-coated plates. Cryopreserved in primary passage as “spheroids,” our high-quality cells are tested:

– Positive for ß-tubulin III (neurons) and GFAP (astrocytes) – Negative for HIV-1, Hepatitis B and C, mycoplasma, bacteria, yeast,

and fungi – Together with media and reagents to ensure they perform optimally

as a system

Sold under license from StemCells, Inc. US patents 5,968,829 and 5,851, 832.

Applications – Transfection – Drug development – Research into neurotoxicity, neurogenesis, electrophysiology,

CNS function, and neurotransmitter disorders

Poietics™ Neural Progenitor Media

Choose from two serum-free media that are specially formulated to support growth, expansion and differentiation of normal human neural progenitor (NHNP) cells:

– For optimal neural progenitor cell growth and differentiation, there’s the NPMM™ Neural Progenitor Maintenance Medium BulletKit™

– For optimal neural progenitor differentiation, we have NPDM™ Neural Progenitor Differentiation Medium BulletKit™

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Primary Cell Case Study: Co-cultures for Neuropathology Research

Co-culture of cryopreserved rat primary cortical and striatal neurons.B. Tinner-Staines, et al. 2003. Society for Neuroscience; Abstract No. 427.15

Background

Most primary neuronal cell cultures use neurons from a single brain region but this does not provide an adequate model to study neuropathologies such as Alzheimer’s or Parkinson’s diseases. De-afferented neurons demonstrate biochemical and physiological abnormalities that limit scientific study and preclude drug screening. To model the circuitry associated with different neuropathologies, relevant neuronal cell types must be co-cultured in vivo.

It is difficult to time and stage co-culture of freshly isolated neurons according to the normal developmental staging of cell connectivity. How-ever, this study shows that cortical and striatal cryopreserved neurons in co-culture could provide viable models for examining questions of basal ganglia function and toxicology.

Method

Cryopreserved primary Clonetics™ Rat Cortical and Striatal Neurons were thawed, plated in tandem, and incubated for 7 – 35 days in vitro. Cultures were characterized using synaptic marker antibodies, receptor and second messenger proteins, and structural marker proteins.

Results

Cortical and striatal co-cultures displayed characteristics of each indi-vidual neuron type. Furthermore, co-cultures showed nerve terminals expressing vesicular GABA transporter or glutamate transporter (latter not shown here), which is evidence of neuronal crosstalk, thus reflect-ing the in vivo situation.

Plating a combination of cryopreserved rat striatal and cortical neurons yields cultures characteristic of both cell types cultured in-dividually, as shown by staining of cell soma (PGP 9.5) and neurofilaments (NF-160).

Thawed cortical and striatal rat neurons were co-cultured for 21 days and stained with antibodies against vesicular GABA transporter (vGAT; green) and dopamine receptor protein (DARPP-32, red). The pres-ence of GABAergic innervation of DARPP-positive neurons gives evidence of cross-innervation between neurons.

Conclusion

Results demonstrate that in vitro modeling of brain circuits can be easily accomplished by using a co-culture of cryopreserved primary neural cells. These facilitate co-cultures according to the normal developmental staging of cell connectivity because researchers do not need to coordinate isolations from different brain regions or embryonic developmental stages.


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Nucleofection™ was the first efficient non-viral transfection technology for primary neurons. Its unique combination of electrical parameters with cell type-specific solutions and protocols gives you up to 70% gene transfer and excellent cell viability.

Now this innovative technology also allows you to transfect primary neurons or glial cells in adherence at later developmental stages.

Amaxa™ Nucleofector™ Technology

Nucleofection™ gives you: – High transfection efficiencies and cell viability – Preservation of cell functionality – A variety of device platforms for different cell numbers and

throughput – Nucleofection™ of primary neurons or glial cells in adherence, even

after several days of culture – Optimized protocols to save time and effort

Transfection of Primary Neural Cells or Cell Lines

Choose the Nucleofection™ Platform that Suits Your Research Needs

Advanced Platform 96-well Add-on High-throughput Platform Basic Device

Device 4D-Nucleofector™ System 96-well Shuttle™ System 384-well Nucleofector™ System Nucleofector™ 2b Device

Unit

Throughput (samples per run) Low to medium (1 – 16) Low to high (1 – 96) High (384) Low (1)

Reaction volume 20 µl + 100 µl 20 µl 20 µl 100 µl

Electrode material Conductive polymer Conductive polymer Conductive polymer Aluminum

Low cell numbers (20 µl) 5 × 104 to 5 × 105 5 × 104 to 5 × 105 5 × 104 to 5 × 105 –

High cell numbers (100 µl) 4 – 5 × 106 – – 4 – 5 × 106

DNA Vector amount/sample

0.2 – 1 μg (20 µl) 1 – 5 μg (100 µl)

0.2 – 1 μg

0.2 – 1 μg

1 – 5 μg

siRNA amount/sample (concentration 2 nM – 2 µM)

0.04 – 40 pmol (20 µl) 0.2 – 200 pmol (100 µl)

0.04 – 40 pmol

0.04 – 40 pmol

0.2 – 200 pmol

Adherent Nucleofection™ n n – –

Compatibility with 96-well Shuttle™ System

n – – –

www.lonza.com/celldatabase – To find transfection data for your cell type of interest.

" Non-viral transfection doesn’t give me the transfer efficiency I’m looking for."

SOLUTION: Our non-viral Nucleofector™ Technology delivers 70% gene transfer efficiency and high expression levels.

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Efficiency*

Viability*

Kit for 4D-Nucleofector™ and 96-well Shuttle™ Systems (name of specific protocol)

Kit for Nucleofector™ II/2b Device

Primary Neural Cells Astrocytes, mouse 55-65% 60-70% P3 Primary Cell (Neuron, Basic) Glial Cell, Basic

Astrocytes, rat 60-70% 70-80% P3 Primary Cell (Neuron, Basic) Glial Cell, Basic

Dorsal root ganglia (DRG), chicken 25-35% P3 Primary Cell (Neuron, Basic) Chicken Neuron

Dorsal root ganglia (DRG), rat 35-45% P3 Primary Cell (Neuron, Basic) Rat Neuron

Neuron, cortical, rat 30-70% 45-60% P3 Primary Cell (Neuron, rat) Rat Neuron

Neuron, hippocampal, chicken 40-50% P3 Primary Cell (Neuron, Basic) Chicken Neuron

Neuron, hippocampal, mouse 50-60% P3 Primary Cell (Neuron, Basic) Mouse Neuron

Neuron, hippocampal, rat 30-70% 45-60% P3 Primary Cell (Neuron, rat) Rat Neuron

Neuron, hippocampal, rat – adherent 30-50% 50-80% Basic Neuron AD (Neuron, Basic, AD) Not Applicable

Neuron, cortical, rat – adherent 30-60% 70-90% Basic Neuron AD (Neuron, Basic, AD) Not Applicable

Neuron, cortical, mouse – adherent 20-70% 70-90% Basic Neuron AD (Neuron, Basic, AD Not Applicable

Oligodendrocyte, rat 40-50% 55-65% P3 Primary Cell (Neuron, Basic) Glial Cell, Basic

Stem CellsNeural stem cell (NSC), mouse 75-85% Primary Cell Optimization Mouse NSC

Neural stem cell (NSC), rat 40-50% Primary Cell Optimization Rat NSC

Neuronal Cell Lines**

AGN2a 60-95% Cell Line Optimization Cell Line R

BV2 55-70% 70-95% Cell Line Optimization Cell Line T

C6 85-95% 70-80% Cell Line Optimization Cell Line V

H4 70-80% 80-90% Cell Line Optimization Cell Line V

IMR-32 75-85% 55-65% Cell Line Optimization Cell Line L

Neuro2A 47-85% 80-95% Cell Line SF Cell Line V

NG108-15 60-70% 75-85% Cell Line Optimization Cell Line V

PC-12 85-95% 75-85% Cell Line Optimization Cell Line V

SH-SY5Y 60-85% 40-80% Cell Line SF Cell Line V

SK-N-MC 60-95% 30-60% Cell Line Optimization Cell Line V

SK-N-SH 80-90% 65-75% Cell Line Optimization Cell Line V

U-87MG 35-45% 85-95% Cell Line Optimization Cell Line T

Primary cells marked blue have Lonza-validated optimized protocols. Cell lines marked blue also have optimized protocols with ATCC® clones.

*Approximate ranges extrapolated from larger result collections, including Lonza and customer data** This is only a selection of cell lines. www.lonza.com/celldatabase – For further neuronal cell lines and protocol guidance.

Transfection Efficiency and Cell Viability with Nucleofector™


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Adherent Nucleofection™ of Primary Neurons at Later Developmental Stages

Transfect primary neural cells at any stage of culture, including late development, without putting them in suspension. Forget about the traditional limitations of electroporation-based methods with our new 4D-Nucleofector™ System that also enables adherent Nucleofection™:

– Achieves transfection efficiencies of up to 70% – Basic Kits help pinpoint optimal conditions for primary neurons, glial

cells or neural stem cells – Has been tested for cryopreserved Clonetics™ Primary Neurons

Applications

4D-Nucleofector™ × Unit or 96-well Shuttle™ Add-on: – Culture cells for up to 14 days in specialized Nucleocuvette™

AD Strips (20 μl) – Transfect them at any time during this culture period – Suited for analysis by light and fluorescence microscopy; absorp-

tion, luminescence, or fluorescence assays; or mRNA expression

4D-Nucleofector™ Y Unit: – Culture cells in standard 24-well plates – For Nucleofection™, simply insert a disposable conductive polymer

24-well Dipping Electrode Array for Nucleofection™ – Ideal for post-transfection analysis by confocal microscopy or

patch clamping

Preserved functionality after adherent Nucleofection™ of neurons. Mouse cortical neurons were cultured in 24-well plates and transfected after 6 DIV with 17.5 μg pmaxFP™-Yellow-C Vector using the Basic Neuron 4D-Nucleofector™ Y AD Kit. Four days post Nucleofection™, cells were immuno-stained for maxFP™-Yellow expres-sion (green) and synapses (red), stained for nuclei (DAPI, blue), and analyzed by fluorescence microscopy. Transfected neurons show normal synapse formation.

Adherent Nucleofection™ of cryopreserved Clonetics™ Rat Hippocampal Neurons. Frozen rat hippocampal neurons were thawed and seeded into 24-well plates. After 2 days, cells were transfected with 17.5 μg pmaxGFP™ Vector using the Basic Neuron 4D-Nucleofector™ Y AD Kit. 1 day post Nucleofection™, cells were analyzed by fluorescence microscopy.

Nucleocuvette™ AD Strips (20 µl)

Dipping Electrode Arrays

Required functional 4D-Nucleofector™ Unit X Unit or 96-well Shuttle™

Y Unit

Pre- and post Nucleofection™ Culture

Nucleocuvette™ Wells

24-well culture plate

Nucleofection™ of cells plated on glass cover slips

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n

High transfection efficiencies and viabilities n n

Cell analysis by: – transmission light or fluorescence microscopy – absorption, luminescence or fluroescence assays – confocal microscopy – patch clamping

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Nucleofection™ of cryopreserved Clonetics™ Primary Animal Neurons

n

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Adherent Nucleofection™ using Nucleocuvette™ AD Strips or 24-well Dipping Electrode Arrays. After isolation, primary neurons are directly plated into poly-D- or poly-L-lysine coated Nucleocuvette™ Strips (when using the × Unit) or 24-well culture plates (when using the Y Unit). Cells can be cultured up to 14 days and transfected by Nucleofection™ at any time during this period.

Adherent Nucleofection™ – All-in-one Process

Up to 14 days

Pre-culture AnalysisNucleofection™

or

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Are neural progenitor cells (NPCs) better suited for transplantation when transfected with neurotrophic factors?Cesnulevicius, et al. 2006. Stem Cells; 24(12): 2776-91

Background

Transplanting neuronal progenitor cells (NPCs) or dopaminergic neu-rons might have therapeutic potential for neurodegenerative diseases, such as Parkinson’s. However, transplanted cells show limited survival and are hard to identify in situ. This study investigated whether NPCs transfected with neurotrophic factors survived and matured more suc-cessfully after transplantation.

The challenge was to find a non-viral transfection method that provides high efficiency without morphological or functional changes.

Method

To identify the best method for transfecting NPCs derived from ventral mesencephali of rat brain, the study tested electroporation, lipofection, and Nucleofection™.

Results

Nucleofection™ was the most efficient method for NPCs, achieving a transfection rate of more than 45%.

Transfection Case Study: Parkinson's Research

Ventral mesencephalic progenitor cells from rat brain were transfected with two differ-ent plasmids expressing DsRed or eGFP using conventional electroporation, lipofection, or Nucleofection™.

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Conclusion

Cells transfected by Nucleofection™ displayed an unaltered morphology, could be differentiated into neurons, and were viable after transplanta-tion. By delivering higher expression levels, Nucleofection™ could help to further investigate the therapeutic potential of NPCs.


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Drug Discovery

Have greater confidence in your data by using assay-ready human neurons to screen your drug candidates.

Human MotorPlate™ Neurons

Save yourself the hassle of species-to-species extrapolation by using the first commercially available source of human motor neurons in your screening campaigns:

– Take advantage of assay-ready human embryonic stem cell- derived motor neurons

– Choose from different maturation stages: NeuroPlate™ Neural Progenitors, early stage MotorPlate™ Standard Motor Neurons or late stage MotorPlate™ Mature Motor Neurons

– Tested for neuronal morphology, specific markers, adherence, and density

– Obtain large quantities with minimal lot-to-lot variation – Improve your workflow with the convenience of ready-to-use,

pre-plated tools – Receive optimized MotorBlast™ Culture Media with your shipment

Applications – Viability, toxicity, and neuronal outgrowth assays – Motor neuron function and disease research – Maturation and cell cycle analysis studies

Standard MotorPlate™ Cells stained for Neuronal Class III ß-tubulin (green) and Glial Fibril-lary Acid Protein (red). Nuclei are counterstained with DAPI (blue).

" I need human model systems to avoid species extrapolation in my drug discovery process."

SOLUTION: Now you can access assay-ready embryonic stem cell-derived pure human motor neurons.

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Human Mouse

Alzheimer n n

Autism n n

Huntington n n

Mood disorder n n

Neurodegeneration n n

Parkinson n n

Free your gene expression experiments from biases and interference with integrated research tools for every step of your workflow. Lonza solutions provide biologically relevant results – from cell proliferation and cytotoxicity assays, to sample preparation and qPCR, all the way through protein confirmation.

StellARray™ qPCR Arrays

StellARray™ qPCR Arrays contain 96 or 384 targeted, pre-validated qPCR assays:

– Choose from more than 150 pre-validated, research area-specific qPCR arrays or configure custom arrays online

– Use with standard qPCR equipment – Perform a comprehensive range of neurobiology experiments – Utilize ΔΔCt analysis, or achieve reliable normalization with

StellARray™ Proprietary GPR™ Software, which automatically identifies the best normalizer amplicons based on lowest variance

Cell Analysis

www.array.lonza.com – For more information on the StellARray™ System and its supporting GeneSieve™ Search Tool or Global Pattern Recognition™ (GPR) Analysis Tool.

Applications – Gene expression profiling – Pathway analysis – Microarray data validation – siRNA knock-down analysis – Biomarker discovery

2D well550 μl sample, or

7 cm IPG strip, 12 μl marker

8+1 well*30 μl sample 12 μl marker

10 well32 μl

12 well20 μl

17 well*14 μl

16 well14 μl

* 8+1-well and 17-well gels are multichannel pipette compatible




10 well32 μl

12 well20 μl

17 well*14 μl

16 well14 μl





10 well32 μl

12 well20 μl

17 well*14 μl

16 well14 μl





10 well32 μl

12 well20 μl

17 well*14 μl

16 well14 μl





10 well32 μl

12 well20 μl

17 well*14 μl

16 well14 μl


Comb Options and Well Volumes

*Compatible with multichannel pipettes

Precast PAGEr™ Protein Gels for Consistent, Reliable Western Blotting

PAGEr™ gels are easy to use precast protein mini-gels that offer sharp resolution and consistent protein transfer. Each lot is functionally tested and shipped fresh every time, for guaranteed shelf life and performance. PAGEr™ gels give you:

– Sharp resolution for crisp separation of proteins 5 – 300 kDa – Marked sample lanes and simple twist open design – Compatibility with most chambers – Multiple well formats and gel concentrations – Tris-Glycine buffer for traditional Laemmli separation

Applications – SDS-PAGE – Native PAGE – Western blotting – Antibody screening

" I can’t find reliable, ready-to-use assay tools for mRNA, protein expression, cell proliferation, or cytotoxicity."

SOLUTION: Choose from our variety of neurology-specific assay tools, including qPCR arrays, ready-to-use protein gels, and bioassay kits to measure cell proliferation and cytotoxicity.

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ViaLight™ Plus Kits

Measure cell proliferation and cytotoxicity by using stable biolumines-cence to detect adenosine triphosphate (ATP):

– Process and analyze a 96-well plate in less than 15 minutes – Detect as few as ten cells; allowing for lower seeding densities

and more assays – Easily perform scalable automation with our simple,

no-shake protocol – Add two reagents directly to your culture and read luminescence – Expand your dynamic range to five decades, in adherent or

suspension cultures – Run this test on a variety of luminometers or

scintillation counters – Avoid radioactive or toxic materials

www.lonza.com /vialight

ToxiLight™ Kits

Check a compound’s cytotoxic effects non-destructively by measuring adenylate kinase (AK) leakage from damaged cells:

– Generate results from as few as 10 cells – Eliminate the need to lyse by monitoring cytotoxicity from

supernatant – Simply add a single reagent directly to cells, or to

a supernatant aliquot – Process and analyze a 96-well plate in less than 10 minutes – Freeze supernatants and return to your work later without losing

AK activity

www.lonza.com/toxilight

Identity Dose-dependent Activities in Cells

Comparison of ViaLight™ Plus and ToxiLight™ Kits using HUVECs dosed with camptothecin. The ATP levels indicated by the ViaLight™ Plus RLUs reduce steadily in a dose-dependent manner. At the lower drug doses, the AK released from the cells is relatively low compared with that of the control, only increasing dramatically at the highest drug doses.

EC 50 Data Generated Using ViaLight Plus Shows Consistency Over Time

HepG2 cells were incubated with the alkylating agent Mitomycin C for 48 hours and the assayed using ViaLight™ Plus. The experimental values are the mean of eight replicant samples read every hour over a 5 hour period. The EC values remain consistent over the 5 hour read period.

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Ordering Information

Cat. No. Description Size

Clonetics™ Primary Animal NeuronsM-Cx-400 Mouse CD1 Brain Cortex Neurons ≥ 4 million cells

M-Cp-402 Mouse CD1 Brain Striatum Neurons ≥ 4 million cells

M-Cx- 300 Mouse C57 Brain Cortex Neurons ≥ 4 million cells

M-Cp-302 Mouse C57 Brain Striatum Neurons ≥ 4 million cells

M-Hi-401 Mouse CD1 Brain Hippocampus Neurons ≥ 1 million cells

R-Cx-500 Rat Brain Cortex Neurons ≥ 4 million cells

R-Hi-501 Rat Brain Hippocampus Neurons ≥ 1 million cells

R-Hth-507 Rat Brain Hypothalamus Neurons ≥ 2 million cells

R-Cp-502 Rat Brain Striatum Neurons ≥ 4 million cells

R-Drg-505 Rat Dorsal Root Ganglion Neurons ≥ 0.2 million cells

R-eDRG-515 Rat Dorsal Root Ganglion Neurons (embryonic) ≥ 1 million cells

R-Cb-503 Rat Cerebellar Neurons ≥ 4 million cells

R-Ret-508 Rat Retinal Cells ≥ 0.2 million cells

Clonetics™ Primary Animal and Human AstrocytesCC-2565 NHA – Normal Human Astrocytes ≥ 1 million cells

M-AsM-430 Mouse CD1 Brain Mixed Astrocytes ≥ 1 million cells

M-AsM-330 Mouse C57 Brain Mixed Astrocytes ≥ 1 million cells

R-CxAs-520 Rat Brain Cortex Astrocytes ≥ 1 million cells

R-HiAs-521 Rat Brain Hippocampus Astrocytes ≥ 1 million cells

R-CpAs-522 Rat Brain Striatum Astrocytes ≥ 1 million cells

R-AsM-530 Rat Brain Cx-Hi-Cp Mix Astrocytes ≥ 1 million cells

Poietics™ Neural Progenitor CellsPT-2599 NHNP – Normal Human Neural Progenitor Cells ≥1.2 million cells

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Clonetics™/Poietics™ Primary Neural Cell Growth MediaCC-4461 PNGM™ Primary Neuron Growth Medium BulletKit™

Includes Basal Medium and SingleQuots™ KitKit

CC-4512 PNGM™-A Primary Neuron Growth Medium – Adult BulletKit™ Includes Basal Medium and SingleQuots™ Kit

Kit

CC-3256 PNBM™ Basal Medium 500 ml

CC-4462 PNGM™ SingleQuots™ Supplement and Growth Factors –

CC-4511 PNGM™-A Primary Neuron Growth Medium – Adult SingleQuots™ Kit Kit

CC-3186 AGM™ BulletKit™ Kit – Includes Basal Medium and SingleQuots™ Kit Kit

CC-3187l ABM™ Basal Medium 500 ml

CC-4123 AGM™ SingleQuots™ Supplements and Growth Factors –

CC-3209 NPMM™ Neural Progenitor Maintenance Medium BulletKit™ Kit

CC-3229 NPDM™ Neural Progenitor Differentiation Medium BulletKit™ Kit

CC-3210 NPBM™ Neural Progenitor Basal Medium 200 ml

CC-4241 Neural Progenitor Maintenance Medium SingleQuots™ Kit (contains hEGF and hFGF)

Kit

CC-4242 Neural Progenitor Supplement SingleQuots™ Kit (contains NSF-1 and GA) Kit

Pluripotent Stem Cell-derived Neuronal CellsFP-6020 NeuroPlate™ Human ESC Neuronal Progenitors 96-well plate

FP-6041 NeuroPlate™ Human ESC Neuronal Progenitors 384-well plate

FP-6011 MotorPlate™ Standard Human ESC Motor Neuron Progenitors 96-well plate

FP-6040 MotorPlate™ Standard Human ESC Motor Neuron Progenitor 384-well plate

FP-6046 MotorPlate™ Mature Human ESC Motor Neuron Progenitors 96-well plate

FP-6049 MotorPlate™ Mature Human ESC Motor Neuron Progenitors 384-well plate

Nucleofector™ DevicesAAB-1001 Nucleofector™ 2b Device –

AAF-1001B 4D-Nucleofector™ Core Unit –

AAF-1001X 4D-Nucleofector™ × Unit –

AAM-1001S 96-well Shuttle™ Device –

Kits for 4D-Nucleofector™ DeviceV4XP-3024 P3 Primary Cell 4D-Nucleofector™ × Kit L (for neurons and glial cells) 24 rxn (100 μl Nucleocuvette™)

V4XP-3032 P3 Primary Cell 4D-Nucleofector™ × Kit S (for neurons and glial cells) 32 rxn (20 μl Nucleocuvette™; 16-well)

V4XP-1A32 Basic Neuron 4D-Nucleofector™ × AD Kit (adherent) 32 rxn (20 μl Nucleocuvette™ AD; 16-well)

V4YP-1A24 Basic Neuron 4D-Nucleofector™ Y AD Kit (adherent) 24 rxn (Dipping Electrode)

V4XP-9096 Primary Cell Optimization 4D-Nucleofector™ × Kit (for neural stem cells) 96 rxn (20 μl Nucleocuvette™; 16-well)

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Kits for 96-well Shuttle™ DeviceVIPI-10003 Basic Neuron 96-well Nucleofector™ AD Kit (adherent) 96 rxn (20 μl Nucleocuvette™; 96-well)

V4SP-3096 P3 Primary Cell 96-well Nucleofector™ Kit (for neurons and glial cells) 96 rxn (20 μl Nucleocuvette™; 96-well)

V4SP-3960 P3 Primary Cell 96-well Nucleofector™ Kit (for neurons and glial cells) 960 rxn (20 μl Nucleocuvette™; 96-well)

V4SP-9096 Primary Cell Optimization 96-well Nucleofector™ Kit (for neural stem cells) 192 rxn (20 μl Nucleocuvette™; 96-well)

Kits for Nucleofector™ II/2b DeviceVAPG-1001 Mouse Neuron Nucleofector™ Kit 10 rxn (100 μl aluminum cuvette)

VPG-1001 Mouse Neuron Nucleofector™ Kit 25 rxn (100 μl aluminum cuvette)

VVPG-1001 Mouse Neuron Nucleofector™ Kit 4 × 25 rxn (100 μl aluminum cuvette)

VAPG-1003 Rat Neuron Nucleofector™ Kit 10 rxn (100 μl aluminum cuvette)

VPG-1003 Rat Neuron Nucleofector™ Kit 25 rxn (100 μl aluminum cuvette)

VVPG-1003 Rat Neuron Nucleofector™ Kit 4 × 25 rxn (100 μl aluminum cuvette)

VAPI-1003 Basic Nucleofector™ Kit for Primary Mammalian Neurons 10 rxn (100 μl aluminum cuvette)

VPI-1003 Basic Nucleofector™ Kit for Primary Mammalian Neurons 25 rxn (100 μl aluminum cuvette)

VVPI-1003 Basic Nucleofector™ Kit for Primary Mammalian Neurons 4 × 25 rxn (100 μl aluminum cuvette)

VAPI-1006 Basic Nucleofector™ Kit for Primary Mammalian Glial Cells 10 rxn (100 μl aluminum cuvette)

VPI-1006 Basic Nucleofector™ Kit for Primary Mammalian Glial Cells 25 rxn (100 μl aluminum cuvette)

VVPI-1006 Basic Nucleofector™ Kit for Primary Mammalian Glial Cells 4 × 25 rxn (100 μl aluminum cuvette)

VAPG-1004 Mouse Neural Stem Cell (NSC) Nucleofector™ Kit 10 rxn (100 μl aluminum cuvette)

VPG-1004 Mouse Neural Stem Cell (NSC) Nucleofector™ Kit 25 rxn (100 μl aluminum cuvette)

VVPG-1004 Mouse Neural Stem Cell (NSC) Nucleofector™ Kit 4 × 25 rxn (100 μl aluminum cuvette)

VAPG-1005 Rat Neural Stem Cell (NSC) Nucleofector™ Kit 10 rxn (100 μl aluminum cuvette)

VPG-1005 Rat Neural Stem Cell (NSC) Nucleofector™ Kit 25 rxn (100 μl aluminum cuvette)

VVPG-1005 Rat Neural Stem Cell (NSC) Nucleofector™ Kit 4 × 25 rxn (100 μl aluminum cuvette)

BioAssay Kits for Cell Proliferation and CytotoxicityLT07-221 ViaLight™ Plus Cell Proliferation and Cytotoxity BioAssay Kit 500 tests

LT07-121 1,000 tests

LT07-321 10,000 tests

LT17-221 500 tests (with 5 white TC plates)

LT17-517 ViaLight™ 100% Lysis Control Set (sold separately) 10 ml (200 tests)

LT07-217 ToxiLight™ Non-destructive Cytotoxity BioAssay Kit 500 tests

LT07-1117 1,000 tests

LT07-117 500 tests (with 5 white TC plates)

18


Cat. No. Cat. No. Cat. No. Cat. No. Cat. No. Cat. No.

Gel Concentration/Separation Range Cassette Size (cm) 2D well 10 well 12 well 16 well 17 well 8 + 1 well

4 – 12% gradient 25 – 250 kDa

9 × 10 10 × 10

– –

58520 59520

58522 59522

58524 59524

– –

– –

4 – 20% gradient 5 – 200 kDa

9 × 10 10 × 10

– 59557

58511 59511

58505 59505

58517 59517

58545 59545

58551 59551

8 – 16% gradient 15 – 200 kDa

9 × 10 10 × 10

– 59564

58519 59519

58521 59521

58523 59523

58560 59560

58562 59562

10 – 20% gradient 5 – 150 kDa

9 × 10 10 × 10

– –

58512 59512

58506 59506

58518 59518

– –

– –

7.5% 50 – 200 kDa

9 × 10 10 × 10

– –

58507 59507

58501 59501

58513 59513

58540 –

– –

10% 25 – 200 kDa

9 × 10 10 × 10

– 59554

58508 59508

58502 59502

58514 59514

58542 59542

58548 59548

12% 20 – 200 kDa

9 × 10 10 × 10

– 59571

58509 59509

58503 59503

58515 59515

58543 59543

– –

15% 10 – 50 kDa

9 × 10 10 × 10

– 59556

58510 59510

58504 59504

58516 59516

58544 59544

58550 59550

PAGEr™ Gels and Accessories


CNS StellARray™ qPCR AssaysPlease Inquire Human Alzheimer’s Disease StellARray™ Various formats

Please Inquire Human Autism StellARray™ Various formats

Please Inquire Human Huntington’s Disease StellARray™ Various formats

Please Inquire Human Mood Disorder StellARray™ Various formats

Please Inquire Human Neurodegeneration StellARray™ Various formats

Please Inquire Human Parkinson StellARray™ Various formats

Please Inquire Mouse Alzheimer’s Disease StellARray™ Various formats

Please Inquire Mouse Autism StellARray™ Various formats

Please Inquire Mouse Huntington’s Disease StellARray™ Various formats

Please Inquire Mouse Mood Disorder StellARray™ Various formats

Please Inquire Mouse Neurodegeneration StellARray™ Various formats

http://array.lonza.com – For StellARray™ qPCR Assay formats.

www.lonza.com/protein – For PAGEr™ Gel formats and accessories.

19

www.lonza.com/researchwww.lonza.com/neurobiology

Lonza Cologne GmbH 50829 Cologne, Germany For research use only. Not for use in diagnostic procedures.StellARray™, GeneSieve™, Global Pattern Recognition™ and GPR™ are trademarks of Bar Harbor Biotechnology. Neurobasal™ and B27® Serum Supplement are registered trademarks of Life Technologies®. ATCC® is a trademark of ATCC™ used under license. All other trademarks herein are marks of the Lonza Group or its affiliates.The information contained herein is believed to be correct and corresponds to the latest state of scien-tific and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or the results to be obtained from the use of such information and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. No statement is intended or should be construed as a recommenda-tion to infringe any existing patent.

© 2011 Lonza Cologne GmbH. All rights reserved.BR-DseCmpgn 03/11 CD-BR014

Contact Information

North AmericaCustomer Service: 800 638 8174 (toll free)Scientific Support: 800 521 0390 (toll free)[email protected]

EuropeCustomer Service: + 32 87 321 [email protected] Support: + 49 221 99199 [email protected]

Online Orderinghttps://shop.lonza.com

InternationalContact your local Lonza DistributorCustomer Service: + 1 301 898 7025, ext. [email protected]

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