NATURAL FIBROUS MATERIALS AS A SOURCE NATURAL FIBROUS MATERIALS AS A SOURCE OF INSPIRATION FOR THE DESIGN OF NOVEL OF INSPIRATION FOR THE DESIGN OF NOVEL

NANOBIOMATERIALSNANOBIOMATERIALS

Anna MitrakiAnna Mitraki

Laboratory of Natural BiomaterialsLaboratory of Natural Biomaterials

Department of Materials Science and Technology, University of Crete,Department of Materials Science and Technology, University of Crete,andand

Institute for Electronic Structure and Lasers, FORTHInstitute for Electronic Structure and Lasers, FORTH

mitraki@materials.uoc.grmitraki@materials.uoc.gr

Our research focuses on: Our research focuses on:

Rational Rational design of protein fibrous materialsdesign of protein fibrous materials from the nano- to the macroscopic scalefrom the nano- to the macroscopic scale

using building blocks inspired from using building blocks inspired from Natural fibrous folds and hard-won biochemical Natural fibrous folds and hard-won biochemical

knowledgeknowledge

50 nm

Crystal structure of a natural fibrous protein made from sequence

repeats (blue squares)

[van Raaij et al. Nature 1999, 401:935]

B

100 nm

[ ]n

50 nm

Peptide sequences as a Peptide sequences as a self-assembly systemself-assembly system

Identification of minimal peptides corresponding to natural building blocks (blue squares)

that can self-assemble intofibrous objects

Papanikolopoulou et al., J. Biol. Chem. 2005, 280 : 2481-2490

Modification of fibrous building blocks towards Modification of fibrous building blocks towards specific functions:specific functions:

-targeting sequences for metallization, cell attachment -targeting sequences for metallization, cell attachment etc, etc, oror

-Conjugation to other organic moieties to form hybrid -Conjugation to other organic moieties to form hybrid materialsmaterials

Metal nanowires fabricated on an octapeptide fibrillar scaffold displaying cysteines

Kasotakis et al., 2009, Biopolymers-Peptide Science, 92:164-72

Example: Example:

…….and.andDevelopment of inter-disciplinary approachesDevelopment of inter-disciplinary approaches

towards controlled positioning on surfacestowards controlled positioning on surfacesand integration in devicesand integration in devices

V. Dinca et al., Nanoletters, (2008) 8 (2): 528-543A. Mitraki and M. Farsari, in : "Nanomaterials  for Life Sciences" series, vol. 15, pp 105-119, Wiley- VCH

Example: peptide fibrils positioned on

a hybrid organic-inorganic material

using femtosecond Laser Technologies

Relevant recent publications:Relevant recent publications:1) 1) Dinca V., et al., (2008) Directed three-dimensional patterning of self-assembled peptide fibrils. Dinca V., et al., (2008) Directed three-dimensional patterning of self-assembled peptide fibrils.

NanolettersNanoletters, , 8: 538-5438: 538-543

2)2) Dinca V., et al., (2008) Fabrication of amyloid peptide micro-arrays using Laser-induced forward Dinca V., et al., (2008) Fabrication of amyloid peptide micro-arrays using Laser-induced forward transfer and avidin-biotin mediated assembly transfer and avidin-biotin mediated assembly Physica Status SolidiPhysica Status Solidi 5: 3576-3579 5: 3576-3579

3)3) Kasotakis, E., et al., (2009) Design of metal-binding sites onto self-assembled peptide fibrils Kasotakis, E., et al., (2009) Design of metal-binding sites onto self-assembled peptide fibrils BiopolymersBiopolymers – –Peptide SciencePeptide Science 92: 164-172 92: 164-172

4)4) Tamamis, P., et al., (2009) Amyloid-like self-assembly of peptide sequences from the adenovirus fiber Tamamis, P., et al., (2009) Amyloid-like self-assembly of peptide sequences from the adenovirus fiber shaft: insights from molecular dynamics simulations. shaft: insights from molecular dynamics simulations. J. Phys. Chem. BJ. Phys. Chem. B. 113: 15639-15647. 113: 15639-15647

5) 5) Mitraki, A. (2010) Protein aggregation: from inclusion bodies to amyloid and biomaterials. Mitraki, A. (2010) Protein aggregation: from inclusion bodies to amyloid and biomaterials. Advances in Advances in Protein Chemistry and Structural BiologyProtein Chemistry and Structural Biology , 79, 79: : 89-12589-125

6)6) Sedman VL., et al., Sedman VL., et al., (2011) Surface-Templated Fibril Growth of Peptide Fragments from the Shaft (2011) Surface-Templated Fibril Growth of Peptide Fragments from the Shaft Domain of the Adenovirus Fibre Protein. Domain of the Adenovirus Fibre Protein. Protein Pept Lett., Protein Pept Lett., 18: 268-27418: 268-274

7) 7) Tiggelaar, SM., et al., (2011). Neutron fibre diffraction studies of amyloid using H(2)O/D(2)O isotopic Tiggelaar, SM., et al., (2011). Neutron fibre diffraction studies of amyloid using H(2)O/D(2)O isotopic replacement.replacement. Acta Crystallogr Sect F Struct Biol Cryst Commun.Acta Crystallogr Sect F Struct Biol Cryst Commun. 67:332-335. 67:332-335.

8)8) Hyttel-Clausen C., et al., Hyttel-Clausen C., et al., (2011) Electrostatic force microscopy of self-assembled peptide structures. (2011) Electrostatic force microscopy of self-assembled peptide structures. Scanning,Scanning, in press in press

9)9) Viguier, B., et al., Viguier, B., et al., (2011) Development of an electrochemical metal-ion biosensor using self-(2011) Development of an electrochemical metal-ion biosensor using self-assembled peptide nanofibrils. assembled peptide nanofibrils. ACS Appl. Mater. InterfacesACS Appl. Mater. Interfaces, in press., in press.

10)10) Charalambidis, G., et al., 2011) Self-assembly into spheres of a hybrid diphenylalanine-porphyrin: Charalambidis, G., et al., 2011) Self-assembly into spheres of a hybrid diphenylalanine-porphyrin: increased fluorescence lifetime, conserved electronic properties. increased fluorescence lifetime, conserved electronic properties. Chemistry Eur. J.,Chemistry Eur. J., in press in press

Acknowledgements UoC:Emmanouil KasotakisErifyli KaloudiDina TerzakiAlexandra Siakouli (EM Facility)Eva Papadogiorgaki (EM Facility)Aleka Manousaki (EM Facility)

Collaborations

- IESL, FORTH CRETE Dr. Maria Farsari, Prof. Costas Fotakis, - Technical University of Denmark (DTU) Prof. Winnie Svendsen, Dr. Jaime Castillo - Institut Laue Langevin in Grenoble, France Prof. Trevor Forsyth, Estelle Mossou - University of Nottingham, England Prof. Saul Tendler, Prof. Clive Roberts, Dr. Stephanie Allen, Dr. Sedman Victoria- University of Santiago de Compostela,Spain Prof. Mark J. van Raaij - University of Tel-Aviv, Israel Prof. Ehud Gazit, Lihi Adler-Abramovich - University of Cyprus Prof. G. Archontis, Dr. Ph. Tamamis- CEA Saclay, France Dr. P. Guenoun, Cr. C. Chevallard

Financial support:

EU “BeNatural” project

“Bioengineered Nanomaterials For Research and Applications” NMP4-CT-2006-033256Coordinated by UoC