national conference on drug discovery & formulation development 2016 by amity abstract cd.pdf

National Conference on Drug Discovery and Formulation Development 23rd February 2016

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus



Chairman

Amity University, Lucknow Campus

Chancellor

Amity University Rajasthan

Amity University Haryana

Dr. Aseem Chauhan President

Amity University Mumbai

It gives me immense pleasure and sense of great pride that Amity Institute of Pharmacy,

Amity University, Uttar Pradesh, Lucknow Campus, is organizing a National Conference on

Drug Discovery & Formulation Development on February 23, 2016.

The National Conference is a step towards discussion and deliberation in sharing

knowledge on innovation and emerging areas in Drug Discovery and related fields. I am sure that

the presence of scientists and researchers from various leading centers of Pharmaceutical Science

in India will lead to a wonderful opportunity to exchange thoughts and ideas on contemporary

issues and will set new goals and targets for young researchers and scientists in the area of Drug

Discovery.

As an obligation towards our society, we must also contemplate how this research can be

applied and commercialized so that India can not only become a front runner in the field of New

Drug Discovery and Formulation Development but also become self-sufficient in the area of

health and medicine.

I wish organizers as well as the participants of this prestigious National Conference, a

great success and hope that ideas emanating from the Seminar will serve to set new frontiers of

knowledge in the subject.

(Dr. Aseem Chauhan)



Convener, NCDDFD 2016

Dy. Director, Amity Institute of Pharmacy

Amity University, Lucknow Campus

Message

It is matter of great pride that the team Amity Institute of Pharmacy is organizing a 3rd

national

scientific event. Drug Discovery with pace is need of hour in current scenario. We are fighting

with deadliest existing diseases and new outbreak like Zika virus infection. The researchers

across the world have no other choice but to work together. The budding students and

researchers need exposure to newer technology and scientific finding.

I am sure that National Conference on Drug Discovery and Formulation Development will serve

its important role in giving a common platform for new generation to interact and share ideas

with eminent scientist and academician. I welcome all the delegates to AMITY and thank you for

making this conference a great success.

(Dr. Sajal Srivastava)



Parmesh K. Dwivedi

(Treasurer-NCDDFD 2016)

I am extremely delighted to greet and welcome you all to National conference on Drug

discovery and formulation development. Theme of the conference has much significance as the

drug discovery and formulation development are the heart of pharmaceutical research. Now a

days, the process for research and development of new medicines is growing in difficulty and

length. Hence, the goal of this conference is to bring together researchers, academicians, industry

persons and learners to exchange their research ideas and results. We look forward to an

exciting day of insightful presentations, discussions and sharing of technical ideas with

colleagues from different parts of our country.

I hope and expect that the theme will result in fruitful and passionate discussions.



Responsibility Matrix:

S.

No

.

Committee

Name

Faculty Incharge/

Co-incharge

Student Name Programme Phone No.

1 Overall

Coordinator

Dr. Sajal Srivastava Ms. Priyanka

Srivastava; Ms.

Shivani Singh

B. Pharm 9839030220

9415054170

2 Marketing Dr. Sajal Srivastava,

Mr. Parmesh K

Dwivedi, Dr. Rahul

Shukla

Ms. Priyanka

Srivastava

B. Pharm 9839030220

3 Invitation Dr. M V Ramana, Dr.

Sajal Srivastava

4 Hospitality Mr. Amrit K Singh Mr.

Prakash Deep

Mr. Shushant

Mishra and Mr.

Anshu Singh

B. Pharm 6th

sem

8853933214

8953591757

5 MC Mr. Saikat Sarkar 1. Ms. Priyanka

Srivastava Ms

Alka Rai; 2. Parth,

Abhishek3. Mr.

Anurag

B. Pharm

B Pharm

M Pharm

9839030220

6 Transport Mr. Rahul Shukla Mr. Shunbam

Pandey Mr. Rahul

Kushwaha

Mr. Vikas

Upadhyay

B. Pharm 9616511037

8174838048

7 Reception Ms Nimisha Ms. Richa

Srivastava;

Ms. Nishita and

Ms Komal

Akansha lal

Ph. D.

B. Pharm

9936824032

8 Hall

Management

Dr . Zeeshan Fatima;

Dr. Himani Awasthi

Ms. Sristi raj and

Shreya

Pooja Yadav

B Pharm

B. Pharm 8th

sem

8102734585

7398517685



9 Disciplinary Mr. P. P. Dash; Ms.

Richa Srivastava

Mr. Rahul Pal and

Mr. Abhishek

Pandey; Ankan

Rastogi and

Shubham

Kesarwani; Mr.

Himanshu Jaiswal

and Mr. Prasoon

Dwivedi

B. Pharm 8726982698

9044999769

8090225216

10 Registration Ms Nimisha Ms. Richa

Srivastava;

Ms. Nishita and

Ms Komal

11 Accommodati

on

Ms. Neha Mathur and

Mr. Saikat Sarkar

Ravindra,

Rajeshwar and

vimal

B. Pharm 8th

sem

9807801157

9795910460

8960239311

12 Poster Display Dr. Mohini Chaurasia Ms. Shivani Singh

Ms Akansha Lal

B. Pharm 8th

sem

9415054170

8899309635

13 Certificate Mr. P K Dwivedi Mr. Vidhu

shekhar Dixit

Mr. Zeeshan

Ahmad

B. Pharm 6th

Sm

7398221508

14 Saraswati

vandana

Dr. Sajal Srivastava Ms. Mini Singh;

Ms Ankita Singh;

Ms. Kashish

Kapoor

B. Pharm 9688623489

15 Printing,

Publishing

Media & Press

Dr. Sajal Srivastava,

Mr. Ashutosh Chaubey

Ms. Rahul

Kushwaha

B. Pharm 7688623419

16 Abstract

Screening

& Printing

Dr. Sajal Srivastava Mr. Himanshu B. Pharm VI 8765812332

17 Rapporteur Ms. Neha Mathur; Ms.

Suchita

Mr. Vimal Misra B. Pharm 8th

sem

8960239311



SL. NO.

AUTHORS POSTER NO.

TITLE

1 LUBNA AZMI,, ILA

SHUKLA, SHYAM

SUNDAR GUPTA,

PADAM KANT,

CH.V.RAO

PO301 ULCER PROTECTIVE EFFECT OF ARGYREIA

SPECIOSA METABLOLITES ON PROBIOTIC

BACTERIA

2 SANGEETA BAJPAI, D.K.AWASTHI

PO302 FORMULATION OF XANTHINE IN THE

TREATMENT OF BRONCHITIS

3 RICHA SRIVASTAVA,

SAJAL

SRIVASTAVA

PO303 LIPOSOMAL DRUG DELIVERY OF

METHOTREXATE

4 PRITT VERMA, SHRAVAN K.

PASWAN, SURYA P.

SINGH, SAJAL

SRIVASTAVA, CH

V.RAO1

PO304 PROTECTIVE EFFECT OF ACACIA NILOTICA

(BARK) AGAINST ANTI TUBERCULAR DRUG

INDUCED HEPATIC DAMAGE AN

EXPERIMENTAL STUDY

5 SHRAVAN K. PASWAN, PRITT

VERMA, ALOK

R.GAUR, ABHISEK

RAJ, CH.V. RAO,

SAJAL

SRIVASTAVA

PO305 ANTIOXIDANT AND WOUND HEALING

PROPERTIES OF PHYLA NODIFLORA AGAINST

EXCISION WOUND HEALING ACTIVITY

6 ILA SHUKLA, LUBNA AZMI,

SHYAM SUNDAR

GUPTA, PADAM

KANT, CH.V.RAO

PO306 ANTI HEPATOTOXIC ACTIVITY OF MORUS

ALBA IN RAT MODEL OF ALCOHOL LIVER

DISEASE

7 MARYAM SARWAT

PO307 LAMP BASED APPROACHES FOR

AUTHENTICATION OF TRIBULUS TERRESTRIS

8 MADAN SWATI

PO308 PHYTOCHEMICAL ANALYSIS AND FREE-

RADICAL SCAVENGING ACTIVITY OF

FLACOURTIA INDICA (BURM.F.) MERR.

9 NESAR AHMAD, NOORUL HASAN,

ZEESHAN AHMAD,

ARUN KUMAR,

SHEIKH

ZOHRAMEENA

PO309 DICLOFENAC SODIUM: TREATMENT OF

RHEUMATIC DISEASES

10 NOORUL HASAN, PO310 INTRANASAL DELIVERY: AN APPROACH TO



NESAR AHMAD,

ZEESHAN AHMAD,

ARUN KUMAR,

SHEIKH

ZOHRAMEENA

FURRUKH AHMAD

BYPASS THE BLOOD BRAIN BARRIER

11 ZEESHAN AHAMD, ARUN KUMAR,

KULDEEP SINGH,

S.P. SINGH, MD.

ZISHAN,

PARAMDEEP

BAGGA

PO311 NANOCOSMETICS: AN OVERVIEW

12 MOHAMMAD AMIR, ZEESHAN

AHMAD, MD.

ZISHAN, ARUN

KUMAR, KULDEEP

SINGH, S.P. SINGH

PO312 PULMONARY DRUG DELIVERY

13 MOHAMMAD ZISHAN, ZEESHAN

AHMAD, VASEEM

A. ANSARI, S. P.

SINGH, FAISAL

SAEED,

MOHAMMAD AMIR

PO313 RECENT TRENDS IN RECTAL DRUG

DELIVERY SYSTEM

14 SAHAR IDRIS, AIJAZ AHMAD, MD.

ZISHAN, ARUN

KUMAR,

MOHAMMAD

AMIR, NAZMA

KHAN

PO314 TARGETED DRUG DELIVERY TO CNS USING

NANOPARTICLES

15 ASHUTOSH PATHAK,

MITHILESH

KUMAR,

HIMANSHU

MISHRA

PO315 CANCER VACCINE: AN INNOVATIVE

APPROACH IN CANCER TREATMENT

16 NEHA MATHUR, RAVI KUMAR,

KANKSHI TIWARI,

SUPRIYA SINGH,

NIKHAT FATIMA

PO316 EVALUATION OF QUALITY CONTROL

PARAMETERS ON VARIOUS BRANDS OF

PARACETAMOL TABLET FORMULATION

17 SAURABH PO317 AN EXCELLENT VEHICLE FOR NOVEL DRUG



SRIVASTAVA,

SHADAB

MOHAMMAD,

SANA FAROOQUI,

DEVENDRA

KUMAR

DELIVERY SYSTEM: BIODEGRADABLE

POLYMERIC NANOPARTICLES

18 KARUNA S. SHUKLA, MONIKA,

SHAILENDRA

PANDEY, POOJA

CHAWLA

PO318 THIAZOLIDINE-2, 4-DIONE & ITS ANALOGUES

AS ANTICANCER MEDIATED BY DEPLETION

OF INTRACELLULAR CALCIUM: A REVIEW

19 MARYAM SARWAT PO319 DNA FINGERPRINTING OCIMUM BASILICUM

AND O. SANCTUM THROUGH RAPD MARKERS

20 MONIKA, KARUNA SHANKER

SHUKLA,

SHAILENDRA

PANDEY, POOJA

CHAWLA

PO320 SIMULTANEOUS ESTIMATION OF

PIOGLITAZONE AND LOVASTATIN BY RP-

HPLC

21 MAYANK KULSHRESHTHA,

MANJUL PRATAP

SINGH

PO321 DOUBLE PYLORUS: AN UNCOMMON

FEATURE OF STOMACH

22 GUNJA SRIVASTAVA,

MANJUL PRATAP

SINGH, ANURAG

MISHRA

PO322 TRITERPENOIDS: A POTENT

HEPATOPROTECTIVE AGENT

23 SHAISTA SUHAIL, KUMUD NIGAM,

SAURABH

SRIVASTAVA

PO323 CURCUMIN AS A DRUG IN TREATMENT OF

ORAL CANCER

24 M.V. RAMANA, KAMAL DUA

PO324 FORMULATION AND EVALUATION OF

METOPROLOL TARTRATE MICROSPHERES

25 SHUBHAM SHARMA, NIMISHA

MISHRA

PO325 MODEL-BASED DRUG DEVELOPMENT:

APPLICABLE TO ONCOLOGY

26 SATYENDRA K RAJPUT,

VARSHALA PAL,

ARUN K SHARMA,

SHYAM SUNDAR

AGRAWAL

PO326 EFFECT OF BUXUS WALLICHIANA BAILL

EXTRACT AGAINST EXPERIMENTAL

INDUCED AMNESIA IN SWISS ALBINO MICE

27 PRATIBHA GUPTA, ANUPUM KUMAR

PO327 FORMULATION DEVELOPMENT &

CHARACTERIZATION OF ETHOSOMAL GEL



SACHAN

APPROVED FOR THE TREATMENT OF

ARTHRITIS

28 MRIDUL RAJEEV, NIKHAR SHUKLA,

MONIKA KAMBOJ

AND RICHA KHARE

PO328 NANO PARTICLES IN DRUGS

29 NIKHAR SHUKLA, MRIDUL RAJEEV,

SMRITI KHARE

AND RICHA KHARE

PO329 ROLE OF BIOTECHNOLOGY IN

PHARMACEUTICALS

30 UPASANA YADAV PO330 IMPACT OF CHITOSAN NANOPARTICLE IN DRUG DELIVERY

31 KALPANA SONWANI,

ASHUTOSH

KUMAR YADAV

PO331 CHEMICAL CONSTITUENTS AND PARTS OF

PLANTS AS IMMUNOMODULATORS

32 POOJA RAO, DHARAMVEER

PO332 CLINICAL BENEFITS OFHERBAL BASED ADAPTOGENS

33 RICHA MISHRA,

ASHUTOSH

KUMAR YADAV

PO333 ANIMAL MODELS FOR THE STUDY OF STRESS

VULNERABILITY RESILIENCE

34 JYOTI GUPTA, MAYANK

KULSHRESHTA

PO334 NATURAL MEMORY BOOSTERS IN INDIAN

HERBAL PLANTS

35 GARIMA SINGH, DHARAMVEER

PO335 CHRONIC UNPREDICTABLE STRESS:A

CLASSICAL MODEL OF DEPRESSION

36 SARASVATEE KUMARI,

ASHUTOSH

KUMAR YADAV

PO336 FLAVONOIDS AS AN ANALGESIC,

ANTIPYRETIC AND ANTI-INFLAMMATORY

AGENT

37 KHUSHABOO SINGH,

DHARAMVEER,

MAYANK

KULSHRESHTHA

PO337 ROLE OF -AMYLOID PROTEIN IN ALZHEIMER DISEASE

38 DEEPA SHUKLA, SAJAL

SRIVASTAVA,

TALHA JAWAID

PO338 A REVIEW ON EFFECT OF ANXIETY AND

STRESS ON MEMORY

39 SACHIN NEEKHRA,

ROHIT VERMA

PO339 EVALUTION OF HEPATOPROTECTIVE

POTENTAIL OF ALCOHOLIC AND AQUEOUS

EXTRACT OF TERMINALIA BELERICA IN RATS

40 DAN BAHADUR ROKAYA, HIMANI

PO340 MECHANISTIC APPROACHES OF DIFFERENT



AWASTHI MODELS OF ALZHEIMERS DISEASE

41 UPASANA YADAV, ARCHNA TIWARI

PO341 METALORGANIC-FRAMEWORK NANOPARTICLE AS A POTENTIAL FOR DRUG

DELIVERY AND IMAGING

42 CHANDNI TANDON, PRITI

MATHUR AND

MANODEEP SEN

PO342 WILDLY GROWING PLANTS AS A SOURCE OF

AN ALTERNATE MEDICINE: A POSSIBLE

DRUG DEVELOPMENT STRATEGY

43 MONA PAL

PO343 JAPANESE ENCEPHALITIS

44 SANNI GANGWAR

PO344 ANTIVIRAL & ANTIRETROVIRAL

45 PARUL TRIPATHI, PARUL JOHRI,

ADITI SINGH

PO345 ATOMIC LEVEL ANALYSIS OF ENVELOP

PROTEIN FROM DIFFERENT STRAINS OF HIV

VIRUS

46 SARITA JHA,

PARUL JOHRI,

MALA TRIVEDI

PO346 PROTEOME WIDE INTERACTION ANALYSIS

OF MITOCHONDRIAL PROTEINS

47 RUCHI YADAV, A.B.PANT, PRACHI

SRIVASTAVA

PO347 MOLECULAR DOCKING OF RESVERATOL

WITH HIGH THROUGHPUT

NEURODEVELOPMENT INJURY DATA

UNLOCKS POTENTIAL DRUG TARGETS

48 CHETAN RASTOGI, MOHD.MUDDASSIR

KHAN, SHRAVAN

PASWAN, PREET

VERMA, ALOK

RANJAN GAUR,

CH. V. RAO, SAJAL

SRIVASTAVA

PO348 EFFECT OF DESMODIUM GANGETICUM ON

ETHYLENE GLYCOL INDUCED

NEPHROLITHIASIS IN RATS

49 MOHD. MUDDASSIR

KHAN, CHETAN

RASTOGI , PREET

VERMA, SHRAVAN

PASWAN, ALOK

RANJAN GAUR, CH.

V. RAO,. SAJAL

SRIVASTAVA

PO449 EFFECT OF BERBERIS ARISTATA ON

STREPTOZOTOCIN INDUCED DIABETIC IN

RATS

50 SHIKHAR VERMA, ABHISHEK GUPTA,

M.V. RAMANA, A.K.S

RAWAT

PO350 HPTLC ANALYSIS FOR THE QUANTIFICATION

OF GALLIC ACID AND QUERCETIN IN



METHANOLIC FRACTION OF LIMONIA

ACIDISSIMA L. FRUITS

51 ANKAN RASTOGI, MUSAB RAFI,

HIMANSHU

JAISWAL

PO351 AN ABSTRACT ON NATURAL ANTIOXIDANTS

52 SHWETA SACHAN, PARUL JOHRI, ADITI

SINGH

PO352 GLYCOSYLATION IN LIPASE ENZYME OF

HOMO SAPIENS AND ITS CORRELATION

WITH CARBON COMPOSITION

53 JYOTI WAGH, SANDEEP,

ASHUTOSH MISHRA,

RAHUL SHUKLA,

SAIKAT SARKAR,

SUMIT GUPTA

PO353 YOGA: LIFE OF SCIENCE

54 ASHUTOSH MISHRA, RAHUL SHUKLA,

SANDEEP

PO354 PREVENTIVE MEASUREMENTS TO STRESS IN

CURRENT ENVIRONMENT

55 ANKITA TRIPATHI, SURAJ NEUPANE

PO355 A REVIEW ON NECROTIZING FASCIITIS-A

LIFE THREATNING INFECTION OF SKIN AND

SOFT TISSUES

56 SANDEEP, ANGSHU BANARJEE,

ABHILASA, RAHUL

SHUKLA,

ASHUTOSH sMISHRA

PO356 EVALUATION OF PERIPHERAL ANALGESIC

ACTIVITY OF BARK OF ANNONA SQUAMOSA

FROM GONDA REGIONs



Invited talk

" An introduction to Drug Discovery by rational approach and

applications of Green Chemistry in Drug Synthesis"

Prof. Dr. J. Saravanan, Principal,

PES College of Pharmacy,50 feet road,

Hanumanthnagar,Bangalore- 560 050,

email : [email protected].

Abstract

Rational drug discovery is a more streamlined process that requires careful consideration of the

target of the drug as well as the drug itself. This method of drug design uses special equipment to

examine the three-dimensional structure of a drug target and then find a compound that can

interact with the target. Rational drug design therefore requires sound knowledge of chemistry as

well as biology, because chemical interactions between drugs and their targets are what

determine whether a drug is biologically active.

Discovery of new drugs by serendipity and random screening is no more appropriate, instead

drug discovery by rational approach involving techniques like bioisosterism , enzyme inhibition

, consideration of Lipinski rule of five and so on.

The term green chemistry is defined as the invention, design and application of chemical

products and processes to reduce or to eliminate the use and generation of hazardous

substances. Green chemistry can diminish the need for other approaches to environmental

protection. Ideally, the application of green chemistry principles and practice renders regulation,

control, clean-up, and remediation unnecessary, and the resultant environmental benefit can be

expressed in terms of economic impact.

Microwave assisted organic synthesis has revolutionized organic synthesis. Small molecules can

be built in a fraction of the time required by classical thermal methods. As a result, this

technique has rapidly gained acceptance as a valuable tool for accelerating drug discovery and

development processes.



PO301

ULCER PROTECTIVE EFFECT OF ARGYREIA SPECIOSA METABLOLITES ON

PROBIOTIC BACTERIA

Lubna Azmi1*

,, Ila Shukla

2, Shyam Sundar Gupta

1, Padam Kant, Ch.V.Rao

1 Pharmacognosy and Ethnopharmacology Division, CSIR-NBRI, Lucknow, India

2 University of lucknow , Lucknow, Uttar Pradesh, India

E-mail: [email protected]

In the present study, the 50 % w/w ethanolic extract of natural occurring medicinal plant

Argyreia speciosa (L.f), sweet (Family Convolvulaceae) was studied for antioxidant, antiulcer

activity and its purified secondary metabolites were assessed for growth promoting effects on

probiotic bacteria Lactobacillus casei. The gastric ulcers were significantly decreased by all

doses of ASE and probiotic combination as compared with the indomethacin (25 mg/kg body

weight) treated group. In the stomach tissues of treated animals, antioxidant levels were

examined. The administration of indomethacin caused a significant decrease in the levels of

superoxide dismutase (SOD), glutathione peroxidise (GPx) and reduced glutathione (GSH), and

a raise in the lipid peroxidation (LPO) level (p < 0.05). The administration of all doses of ASE

reversed the trend, call to mind a significant increase of SOD, GSH and GPx levels and a

reduction in LPO level in tissues. However, catalase (CAT), glutathione reductase (GR) and

myeloperoxidase (MPx) activities, increased by indomethacin, were found to be lower in the

ASE, probiotic combination of ASE + L.casei and omeprazole-treated groups. The gastric

mucosal constitutive NO synthase (cNOS) and inducible NO synthase (iNOS) activities were

also investigated in tissues of ASE (100 mg/kg), ASE (100 mg/kg) + L. casei (10-8

con.),

omeprazole and indomethacin-treated rat groups. The administration of ASE + L.casei and

omeprazole increased the cNOS activity and lowered the iNOS activity as compared with

indomethacin-treated group. As far as the growth promoting effects of ASE metabolites on L.

casei is concerned, querecetin showed high growth stimulating activity in increased dry matter of

biomass. At low pH growth promoting activity of metabolite was found stable.

Keywords: Argyreia speciosa, Antioxident activity, Antiulcer activity, Indomethacin



PO302

FORMULATION OF XANTHINE IN THE TREATMENT OF BRONCHITIS

Sangeeta Bajpai*1, D.k.Awasthi

2

1School of Applied Sciences Amity University Lucknow U.P.

2Department of Chemistry Sri J.N.P.G. College Lucknow U.P.

Email: [email protected]

Methylxanthines are used for the preparation of group of drugs. These are playing very important

for smoothing of bronchial muscles and stimulate central nervous system (CNS).The

bronchodilator effects of such drugs are applied in the treatment in acute asthma. Such drugs are

also used in the treatment of aponea and have diuretic effect. Oxpentifylline, is a Xanthine

derivative acts as vasodilator. Naturally occurring are caffeine, theophylline and theobromine.

Their derivatives can be used in the improvement form for the treatment of asthma. Modified

forms are Diprophylline, Etamiphylline Camsylate, Proxyphylline and Hydroxytheophylline,

Etofylline. They can be prepared and sell into the market as tread names. Aminophylline,

Aminophylline Hydrate, Choline Theophyllinate. Theobromine is also used in the treatment of

angina pectoris and hyper tension. The basic nucleus is xanthine. It can be substituted by various

groups for the preparation more effective drugs. Now days air pollution is fast growing and

Indian citizens are suffering from respiratory problems. More emphasis is required on bronchial

drugs and treatment of bronchitis, asthma, emphysema and acute respiratory infection .we feel

among such drugs Aminophylline would be better. It can be given orally as well as through

injection.



PO303

LIPOSOMAL DRUG DELIVERY OF METHOTREXATE

Richa Srivastava*, Sajal Srivastava

Amity Institute of Pharmacy, AUUP, Lucknow Campus

Various advanced drug delivery systems such as nanoparticle which include polymeric micelles,

dendrimers , liposomes, SLN, have been investigated to overcome the limitation of the

conventional systems. Considering effectiveness of drug therapy of methotrexate (MXT), the study

focused on formulation of liposomes. The objective of this study was to achieve desired

entrapment of methotrexate in liposome. Liposomes were prepared by thin film hydration method

The liposome were prepared and characterized by, zeta potential for surface charges and particle

size analysis done by Malvern nanosizer and average particle size and zeta potential was found

to be 169 nm and -16.9mV respectively. Liposomal formulations as carrier system for MTX

destined for treatment of cancer by passive targeting Dynamic in vitro drug release was carried

out using phosphate buffer pH 7.4. A short term stability studies was conducted for 2 months at

40C and at room temperature (25

0C). Final formulations were prepared after lyophilizing the

selected liposomes.. MTX entrapped in the liposomes and the entrapment efficiency was found

to be 83 %. The drug loading and entrapment efficiencies were evaluated by a HPLC method

(C18 column 5m, 150mm4.6mm,50mM ammonium acetate/acetonitrile mobile phase v/v 3.0

ml/min flow rate and 256 nm UV detection).The evaluation studies of different processing

variables like drug to lipid ratio, soya lecithin to cholesterol ratio, temperature were evaluated .

KEY WORDS: Liposomes, lyophilization, drug release.



PO304

PROTECTIVE EFFECT OF ACACIA NILOTICA (BARK) AGAINST ANTI

TUBERCULAR DRUG INDUCED HEPATIC DAMAGE AN EXPERIMENTAL STUDY

Pritt Verma*1,2

, Shravan K. Paswan1,2

, Surya P. Singh1, Sajal Srivastava

2, Ch V.Rao

1

1Pharmacognosy and Ethnopharmacology Division, CSIR- BRI, Lucknow, India.

2Amity Institute of Pharmacy, Amity University, Lucknow Campus, India.

Rats were divided into five different groups (n=6), the group I served as a control, Group II

received Isoniazid-INH and rifampicin-RIF (50mg/kg) in sterile water, group III and IV served

as a treatment and received 200,400 mg/kg of 50% ethonolic extract of A. nilotica, and group V

served as standard group and received silymarin (100mg/kg). All the treatments were given for

10-28 days and after rats were authorized, blood and liver were collected for biochemical and

histopathological studies, respectively. The 50% ethanolic bark extract of A. nilotica (200, 400

mg/kg p.o.) showed the remarkable hepatoprotective effect against Isoniazid-INH and

rifampicin-RIF induced hepatic damage, and observed that it shows no any significant change in

a normal posture, behavior and body weight in Wistar rats. The degree of protection was

measured by biochemical and antioxidant parameters such as serum glutamate oxaloacetate

transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase

(ALP), total bilirubin, and the histopathological profile of liver also indicated the

hepatoprotective nature of this drug. The bark extract of A. nilotica has showed dose dependent

activity, among which at the dose level of 200 & 400 mg/kg. The further investigations, the bark

extract of A. nilotica identify the active constituents responsible for hepatoprotection.

Keywords: A. nilotica, Antioxidant, Isoniazid-INH, Rifampicin-RIF, Silymarin.



PO305

ANTIOXIDANT AND WOUND HEALING PROPERTIES OF PHYLA NODIFLORA

AGAINST EXCISION WOUND HEALING ACTIVITY

Shravan K. Paswan1,2

*, Pritt Verma1,2

, Alok R.Gaur1, Abhisek Raj

1, Ch.V. Rao

1, Sajal

Srivastava2

1Pharmacognosy and Ethnopharmacology Division, CSIR- BRI, Lucknow, India.

2Amity Institute of Pharmacy, Amity University, Lucknow Campus, India.

Leaf extract of Phyla nodiflora was developed as ointment (5% & 10% w/w) with easy ointment

base B.P. The ointment was then evaluated for excision wound healing activity. Parameters as

well as antioxidant, measurement of wound space &, wound contraction index, a measurement of

tensile strength and histopathological examination content were determined. Remarkable wound

healing activity was observed with the 10% (w/w) ointment of 50% ethonolic leaf extract of

Phyla nodiflora. Statistical analysis was performed by one-way analysis of variance followed by

t-test. Wound treated with 5% and 10% (w/w) 50% ethonolic leaf extract ointment exhibited

significant excision wound and antioxidant parameter, the healing activity of the excision wound

as evidenced by increased wound contraction, shorter epithelization time, higher tissue breaking

strength and increased hydroxyproline content. This plant has important biological activities and

responsible for the antioxidant and wound healing properties. The study provided sufficient

evidences that Phyla nodiflora might be indeed potential sources to treat many diseases.

Keyword-: Phyla nodiflora, Antioxidant, Histopathological, wound-space, wound contraction.



PO306

ANTI HEPATOTOXIC ACTIVITY OF MORUS ALBA IN RAT MODEL OF ALCOHOL

LIVER DISEASE

Ila Shukla1*, Lubna Azmi

2, Shyam Sundar Gupta

1, Padam Kant

2, Ch.V.Rao

1

1 Pharmacognosy and Ethnopharmacology Division, CSIR-NBRI, Lucknow, India

2 University of lucknow , Lucknow, Uttar Pradesh, India


Alcoholic liver disease has a known aetiology but a complex and incompletely known

pathogenesis. It is an extremely common disease with signicant morbidity and mortality.

Therapy for treatment of it comes with lot of side effects. Presently there is a need of some

natural therapy with lesser side effects and more potential to treat the disease.

Present study is designed with an objective to investigate the antioxidant and

hepatoprotective activities of the ethanolic extract of the Morus Alba in rat model of alcohol

liver disease. Rats were divided into four groups of six each. Group-1 (control) is isocaloric

pairfed, Group-2 received ethanol at a dose of 6g/kg/day, Group- 3 and 4 were administered

extract of Morus Alba as an aqueous suspension orally along with alcohol. After eight weeks,

liver weight, liver function serum markers such as alkaline phosphatase (ALP), aspartate

transaminase (AST) and alanine transaminase (ALP) and lipid peroxidation were measured.

Aqueous suspension showed significant decrease in the levels of AST, ALT and ALP as

compared to alcohol-fed rats. Extract of Morus Alba ameliorated alcohol induced liver injury.

Keywords : Morus Alba, Hepatoprotective, Alcohol Liver Disease, Antioxidant, lipid

peroxidation.



PO307

LAMP BASED APPROACHES FOR AUTHENTICATION OF TRIBULUS TERRESTRIS

Maryam Sarwat*

Pharmaceutical Biotechnology, Amity Institute of Pharmacy, Noida, UP 201303

E mail: [email protected]; [email protected]

Plant based medicines are popular worldwide due to their negligible side effects. There is no

control over the quality of the raw material for medicinal plant use. There are various incidences

of adulteration of medicinal plants with useless weeds. Keeping this in mind, it is imperative to

authenticate the plant material before being used.

Our study plant Tribulus terrestris (Zygophyllaceae), has utmost medicinal importance in curing

urinary discharges, cough, asthma, pain, spermatorrhoea, ophthalmia, anemia, dysentery, skin

and heart diseases; The original use for T. terrestris extract was as a tonic to treat sexual

dysfunction. The plant get often confused with Kallstroemia parviflora and K. californica,

because of their similar morphology. These plants, often get accidentally mixed with T. terrestris

while collecting raw material for herbal drug preparations, making them less efficient or some

times toxic. Samples of this plant were collected from different geographical locations in India.

Random amplified polymorphic deoxyribonucleic acid (DNA) analysis of collected samples was

carried out with 24 random primers. A 510-bp DNA fragment, common to all accessions, was

eluted, cloned, and sequenced. Four LAMP primers were designed on the basis of sequence of

510 bp DNA fragment. LAMP reaction was performed at 65C for 1 h. The resulting amplicon

was visualized through SYBR Green I. LAMP using a primer set for T. terrestris and total DNA

extracted from T. terrestris leaves (0.510.0 ng) as template was detected up to 70 min. On the

other hand, in the reaction using a primer set for T. terrestris and total DNA from K. parviflora

as template, no amplifications were detected. The same tendency could be seen in the reactions

using a set of primers for K. parviflora. LAMP enabled not only identification but also detection

with high specificity. The data showed confirmatory results. Since the assay method is simple,

sensitive, and cost-effective, it is a feasible method for identifying and authentication of C.

roseus.

Keywords: Medicinal plants, authentication, Tribulus terrestris, trnk, LAMP based markers.



PO308

PHYTOCHEMICAL ANALYSIS AND FREE-RADICAL SCAVENGING ACTIVITY OF

FLACOURTIA INDICA (BURM.F.) MERR.

Madan Swati*

Amity Institute of Pharmacy, Amity University, Noida (U.P), India

Flacourtia indica (Burm.f) Merr. Syn. F. ramontchi L Herit (Flacourtiaceae) is a small spinous

tree or shrub found distributed throughout the Himalayas, northern districts of Uttar Pradesh,

Assam, Bengal and Orissa. The roots and fruits of F. indica are used in traditional medicine as a

diuretic, hepatoprotective and antidiabetic. Flacourtia indica dichloromethane (DCM) stem bark,

butanol (BuOH) stem bark, methanol (MeOH) stem bark and MeOH leaf extract were screened

for in vitro antioxidant activity using different models. Subsequent quantification showed the

presence of 15.62 and 11.53 % w/w phenolics; 1.15 and 1.80 % w/w of flavonol in methanol

extract of stem bark and leaf of Flacourtia indica (F. indica) respectively. The methanolic

extract of stem bark and leaf of F. indica showed an effective DPPH radical scavenging activity

with low IC50 values of 17.5 and 21 g/ml respectively. Butanolic extract of F. indica stem bark

showed nitric oxide radical inhibition activity with IC50 value of 28.5 g/ml. The methanolic

extract of stem bark showed hydroxyl radical scavenging by p-NDA method with IC50 value of

350.23 g/ml.

Key words: Flacourtia indica, DPPH, Nitric oxide radical inhibition assay, Scavenging,

hydroxyl radical, p-NDA



PO309

DICLOFENAC SODIUM: TREATMENT OF RHEUMATIC DISEASES

Nesar Ahmad*, Noorul Hasan, Zeeshan Ahmad, Arun Kumar, Sheikh Zohrameena

Integral University, Lucknow

E-Mail- [email protected]

Diclofenac is the medicine works by reducing substances in the body that causes pain and

inflammation. Diclofenac used to treat mild to moderate pain, or signs and symptoms

of osteoarthritis or rheumatoid arthritis. The Cataflam brand of this medicine is also used to treat

menstrual cramps. Diclofenac 75 to 150mg daily (25 to 50mg 3 times daily) is comparable in

efficacy with ordinary aspirin 3 to 5g daily and indomethacin 75 to 150mg daily in rheumatoid

arthritis and with indomethacin in osteoarthritis Diclofenac powder (Cambia) is used to treat

a migraine headache attack. It will not prevent headaches or reduce the number of attacks not use

Diclofenac. If patient have a history of allergic reaction to aspirin or NSAIDs it can increase the

risk of fatal heart attack or stroke, especially if he use it long term or take high doses, if he

suffering from heart disease. Although there is some evidence of Diclofenac efficacy when

administered twice daily, or once daily as a slow release tablet. The drug is also available as

suppositories and ampoules for intramuscular injection. It is not recommended just before or

after heart bypass surgery (coronary artery bypass graft or CABG). These conditions can occur

without warning while you are using this medicine, especially in older adults. No one of the non-

steroidal anti-inflammatory agents is the most suitable drug for all patients requiring such

therapy, and Diclofenac should be considered along with other drugs of its type in the arthritic

patient.



PO310

INTRANASAL DELIVERY: AN APPROACH TO BYPASS THE BLOOD BRAIN

BARRIER

Noorul Hasan*, Nesar Ahmad, Zeeshan Ahmad, Arun Kumar, Sheikh Zohrameena Furrukh

Ahmad

Faculty of Pharmacy, Integral University, Lucknow

E-Mail:- [email protected]

The blood brain barrier (BBB) represents one of the strictest barriers of in- vivo therapeutic drug

delivery. The barriers are restricted exchange of hydrophilic compounds, small proteins and

charged molecules between the plasma and central nervous system (CNS). For decades, the BBB

has prevented the use of many therapeutic agents for treating Alzheimers disease, stroke, brain

tumor, head injury, spinal cord injury, depression, anxiety and other CNS disorders. The

emerging approach is to bypass the BBB by intranasal delivery, which provides a practical, non-

invasive, rapid and simple method to deliver the therapeutic agents to the CNS. This method

works the unique connection between the nose and the brain that has evolved to sense odors and

other chemical stimuli. On the basis of clinical trials (Phase I and II) it is reported that the

intranasal route is feasible for the transport of the drug to the CNS. Intranasal delivery does not

require any modification of the therapeutic agents and does not require that drugs be coupled

with any carrier like in case of drug delivery across the BBB. A wide variety of therapeutic

agents, including both small molecules and macromolecules can be successfully delivered,

including to the CNS, using the intranasal method. Advantage of intranasal delivery are

Bypasses the BBB and targets the CNS, reducing systemic exposure and thus systemic side

effects and Avoids destruction in the gastrointestinal tract, hepatic first pass elimination and

gut wall metabolism, allowing increased, reliable bioavailability.

Key words: Alzheimers disease; Acetylcholine esterase, Buxus wullichiana; Locomotor,

Scopolamine, Donepezil



PO311

NANOCOSMETICS: AN OVERVIEW

Zeeshan Ahamd*, Arun Kumar, Kuldeep Singh, S.P. Singh, Md. Zishan, Paramdeep Bagga

Faculty of Pharmacy Integral University, Lucknow

E Mail: - [email protected]

Platus, a roman philosopher words, A woman without paint is like food without salt."

Nanocosmetics are any cosmetic containing nanoparticles. Nanoparticle is any entity with all

three external dimensions in the nanosize. The magnitude of size of particles in the range of 1 nm

to 100 nm is considered as nanoscale. Nanocosmetics was actually the first branch of

nanotechnology to be commercialized and marketed to consumers, according to Davis Baird.

Antiaging products are the most common example of nanocosmetics, with nanoparticles that go

beneath the surface of the skin and reduce the appearance of lines and wrinkles by reacting with

the body. Various forms of nanotechnology involved in cosmeceuticals like Nanoparticle,

Nanoemulsions, Bucky balls, Nanogel, Microgels Liposomes, Dendrimers, Cubosomes, Solid

Lipid Nanoparticles (SLNs). Recent researches focusing on cosmeceutical products highlighted

strong growth perspectives in the coming years. According to them expanding at a rapid

compound annual growth rate of 7.7%, the global cosmeceutical market will reach $31.84 billion

by 2016. The global cosmeceutical market offers huge potential among the Asian countries, such

as Japan, China, and India which are set to attract major players in the future. Japan has already

made a remarkable position in the global cosmetics market and its position in the cosmeceutical

segment is effectively improving. A report, Cosmeceuticals market to 2018, forecasted that the

global cosmeceuticals market will reach $42.4 billion by 2018.



PO312

PULMONARY DRUG DELIVERY

Mohammad Amir*, Zeeshan Ahmad, Md. Zishan, Arun Kumar, Kuldeep Singh, S.P. Singh


Email: - [email protected]

The respiratory tract is one of the oldest routes used for the administration of drugs. Over the

past decades inhalation therapy has established. It is a valuable tool in the local therapy of

pulmonary diseases such as asthma or COPD (Chronic Obstructive Pulmonary Disease). This

type of drug application in the therapy of these diseases is a clear form of targeted drug delivery.

Currently, over 25 drug substances are marketed as inhalation aerosol products for local

pulmonary effects and about the same number of drugs are in different stages of clinical

development. Pulmonary delivered drugs are rapidly absorbed except large macromolecules

drugs, which may yield low bioavailability due to enzymatic degradation and/or low mucosal

permeability. Pulmonary bioavailability largely depends on the physical properties of the

delivered protein and it is not the same for all peptide and protein drugs. Pulmonary drug

delivery is important researches are an impacts the treatment of illnesses including asthma,

chronic obstructive pulmonary disease and various diseases. Inhalation gives the most direct

access to drug target. In the treatment of obstructive respiratory diseases, pulmonary delivery can

minimize systemic side effects, provide rapid response and minimize the required dose since the

drug is delivered directly to the conducting zone of the lungs.



PO313

RECENT TRENDS IN RECTAL DRUG DELIVERY SYSTEM

Mohammad Zishan, Zeeshan Ahmad, Vaseem A. Ansari, S. P. Singh, Faisal Saeed,

Mohammad Amir

Faculty of Pharmacy, Integral University, Lucknow U.P. India-226026

E mail: - [email protected]

There are many routs to deliver drugs into the body viz oral (through swallowing), sub mucosal

(through buccal and sublingual mucosa), parenteral (through injection), transdermal (through

skin), pulmonary (through inhalation) etc. Among these deliveries rectal routs is widely

accepted. Rectum delivery uses the rectum as a rout of administration for medication and other

fluids which are absorbed by the rectums blood vessels and flow into the bodys circulatory

system which distributes the drugs to the bodys organs. There are many advantages of the rectal

drug delivery system it avoidance of GI metabolism and degradation. The rectal route of

administration is useful for patients who cannot swallowed, Avoidance of irritation to the

stomach, partial avoidance of first pass metabolism, Rapid absorption of many low molecular

weight drugs. Prolonged effect, Absorption enhancement, Rate control drug delivery .There are

many drugs which are available in the market which are applied to rectal route like creams, gels,

suppositories, suspensions and rectal aerosols. Suppositories are available in two forms like solid

suppositories, liquid suppositories, Suppositories are medicated solid bodies suitably shaped for

rectal administration rectal drug delivery systems used the deliver the drug by using

mucoadhesive polymers in through the mucous of the rectum. Rectal drug delivery is so

medicated when the oral medication is not possible.



PO314

TARGETED DRUG DELIVERY TO CNS USING NANOPARTICLES

Sahar Idris*, Aijaz Ahmad, Md. Zishan, Arun Kumar, Mohammad Amir, Nazma Khan


Nano technology plays a unique and pivotal role in the development of brain specific drug

delivery, imaging and diagnosis. Targeted drug delivery is a means of concentrating dugs at a

specific site relative to other parts of the body. It spares the rest of the body from toxic effects of

the drug and is also a potential means of improving therapeutic index. The delivery of drugs to

the CNS is of prime importance for treating specific neurological disorders and various diseases

such as Meningitis, Encephalitis, degenerative diseases such as Alzheimer, Parkinsons and

tumors such as Glioblastoma. The major problem in treating such CNS disorders is due to their

inability to surpass the natural CNS protective barriers, mainly the Blood Brain Barrier and the

Blood cerebrospinal fluid Barrier. Nanoparticle systems in CNS targeted drug therapy provide

better penetration of therapeutic and diagnostic agents, and a reduced risk in comparison to

conventional treatments. By using nanotechnology it is possible to deliver the drug to the

targeted tissue across the BBB, release the drug at a controlled rate, and avoid degradation

processes. Different types of nanoparticles used for CNS targeted drug delivery like inorganic

nanoparticles, Polymeric Nanoparticles, Solid Lipid Nanoparticles, Nano crystals, Carbon

nanotubes, Dendrimers, Quantum Dots, Gold Nanoparticles and Magnetic Nanoparticles.



PO315

Benzothiazole: Different Methods of Synthesis and Diverse Biological

Activities

Desh Deepak Pandey*, Dr Dilip Kumar Pal

Rameshwarm Institute of Technology and Management Lucknow

Substituted 1, 3-benzothiazole derivatives are an important class of heterocyclic compounds. In

recent years heterocyclic compounds analogues and derivatives have attracted strong interest due

to their biological and pharmacological properties. The benzothiazole nucleus containing

compounds involved in research aimed at evaluating new products that possess biological

activities, such as antimicrobial, anticancer, antifungal, anthelmintic, anti-diabetic, amyloid

imagining agents and anticancer agents. The present review focus on the different methods of

synthesis of substituted benzothiazoles with potential activities that are now in developing phase.

Benzothiazole is a heterocyclic compound, weak base, having varied biological activities and

still of great scientific interest now a days. They are widely found in bioorganic and medicinal

chemistry with application in drug discovery. Benzothiazole moites are part of compounds

showing numerous biological activities such as antimicrobial anticancer anthelmintic anti-

diabetic activities.

Keywords: Substituted benzothiazole derivatives, antibacterial activity, anticancer activity, anti-

diabetic activity.

4

5

9

6

8

7

N3

2

S1

1,3-benzothiazole

Chemicals and other reagents required for the synthesis will be procured from standard company

sources. Compounds will be synthesized by using standard procedures. The reactions will be

monitored by TLC and purification of the compounds will be carried out by recrystallization method

using suitable solvent. The synthesized compounds will be characterized by preliminary laboratory

techniques such as melting point, boiling point etc. Compounds synthesized will be confirmed by

FTIR, Mass Spectroscopy and NMR spectral data.

N

S

R

R1



PO316

CANCER VACCINE: AN INNOVATIVE APPROACH IN CANCER TREATMENT

Ashutosh pathak*, Mithilesh Kumar, Himanshu Mishra

Kamla Nehru Institute of Management & Technology, Sultanpur (U.P)

Cancer became a big question for scientific community as no existing treatments could

solve this dreadful disease. Research is in well progress since half century but it failed to give a

right solution to fight against it. One such mile stone treatment for cancer that is giving good

hope to the people is cancer vaccines. Cancer vaccines are made from the persons own cancer

cells or from cells that are grown in a laboratory. Tumor vaccines are often based on DNA

construct viral vectors and cytokines that have been determined as safe. From previous clinical

trials Peptide vaccines are generally safe so long as the cytokine adjutants are used in

combinations and doses previously determined to be safe. A patient with pulmonary metastasis

of colon cancer was treated with artificially synthesized helper/killer-hybrid epitope long peptide

(H/K-HELP) of MAGE-A4 cancer antigen. The patient was vaccinated with MAGE-A4-H/K-

HELP combined with OK432 and Montanide ISA-51. There were no severe side-effects except

for a skin reaction at the injection site.

Antigen based tumor vaccines do not involve the insertion of modified tumor cell or immune cell

into the body. Instead, purified tumor proteins (antigens) are injected to stimulate the patients

antigen presenting cells (APCs) to take up the antigen and present it to T cells.

Keywords: Antigen presenting cells (APCs), cytokines, Peptide vaccines, Montanide



PO317

EVALUATION OF QUALITY CONTROL PARAMETERS ON

VARIOUS BRANDS OF PARACETAMOL TABLET FORMULATION

*Neha Mathur, Ravi Kumar, Kankshi Tiwari, Supriya Singh, Nikhat Fatima

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow, India.

Paracetamol (Acetaminophen) is a widely used OTC medicine available as compressed tablets. It is

marketed and manufactured by a number of pharmaceutical companies and is widely used as an

analgesic, antipyretic medicine. In the present study we selected three brands of Paracetamol tablets

(Brand X, Brand Y and Brand Z) which are easily available and commonly used for analgesic and

antipyretic action. Further, the purpose of the present study was to perform the various quality

control tests of the compressed tablets like, weight variation, hardness, friability, disintegration and

dissolution as per the official monographs B.P, U.S.P and evaluate these three different brands. As a

result of this study we found that all the three brands met the specifications laid down in the

official monographs. They differ only slightly in terms of various quality control parameters,

brand Z disintegrated faster, had faster dissolution rate and so its % drug release was more than

the other two brands at the same time.



PO318

AN EXCELLENT VEHICLE FOR NOVEL DRUG DELIVERY SYSTEM:

BIODEGRADABLE POLYMERIC NANOPARTICLES

Saurabh Srivastava1*

, Shadab Mohammad1, Sana Farooqui

1, Devendra Kumar

2

1Department of Oral Pathology & Microbiology, King Georges Medical University Lucknow, Uttar Pradesh, India

2Department of Pharmacology and Therapeutics, King Georges Medical University, Lucknow, Uttar Pradesh, India

Email: [email protected]

Nanotechnology is new concept, it has been included biomedical, drug delivery, diagnostic,

imaging and mainly focusing in treatment and diagnosis of cancer & other disease state.

Nanoparticles (NPs) have unique and specific properties in terms of chemical and biological by

their small size ranges (1-100 nm) to have a surface area to volume ratio, which allows them to

combine, absorb and hold other compounds like drugs molecules, DNA, RNA, proteins.

Furthermore, their small size, shape and surface and other favourable characteristics helps the

drug therapy to become more stable, targeted, carrier orientated and incorporate with both

hydrophilic and hydrophobic substances. Biodegradable polymeric nanoparticles are the carriers

or vehicle where therapeutic agents can be encapsulated in their matrix and conjugated for

targeted delivery of a number of drugs. The site specific properties of biodegradable polymer as

nanoparticles reduce local pH and affecting cells microenvironment. There are many

biodegradable polymers use for drug delivery and classified synthetic, examples are poly (lactic

acid), poly (caprolactone), poly (glycolic acid), poly (lactic- co- glycolic acid), poly

(trimethylene carbonate), poly (butylenes succinate), poly (p-dioxanone), poly (butylenes

terephthalate), degrapol, hybrane, poly [(caboxyphenoxy) propane-sebacic acid], poly [bis

(hydroxyethyl) terephthalate-ethyl orthophosphorylate terephthaloyl chloride], poly (ethylene

glycol), poly (ortho esters), tyrosine derived, polycarbonate and semi-synthetic polymers are

poly (- hydroxyalkanoate), poly (hydroxybutvrate), poly (hydroxybutyrate-co-hydroxyvalerate)

and natural polymers are collagen, albumin, chitosan, gluten, hyaluronate, cellulose, alginate,

starch. These biodegradable polymers are non-toxic and completely eliminated and degradation

occurs by oxidation enzymatic reaction or hydrolysis. So that it is very safe and effective vehicle

for delivering drug nanoparticles by increasing the degradation rate of polymeric matrix for

targeted delivery of drugs within the effected cells.



PO319

DNA Fingerprinting Ocimum basilicum and O. sanctum through

RAPD Markers Maryam Sarwat*

Pharmaceutical Biotechnology, Amity Institute of Pharmacy, Noida, UP 201303

E mail: [email protected]; [email protected]

India is a hotspot of biodiversity and is home to a wide variety of medicinally and economically

important plants. A number of techniques were being used from time immemorial for correct

identification of medicinal plants. Molecular markers being independent of any environmental

cues or developmental stage are proved to be very helpful in distinguishing valuable medicinal

plants from their adulterants. Ocimum from Lamiaceae is considered as queen of herbs. Most of

its species are used in traditional medicinal system against cough, cold flu, head and ear

infection, arthritis, rheumatism, malaria, fever, allergies and various skin diseases.

Leaf samples were collected from Lucknow, Delhi, NOIDA, Jaipur and Jammu. We have tested

20 RAPD primers, out of them 12 primers were selected for fingerprinting studies. The reaction

was carried out according to Sarwat et al. (2008). The amplification products were scored for the

presence (1) and absence (0) of bands across the genotypes to generate a binary matrix. The

binary matrix was analyzed using the NTSYS-pc to calculate the similarity values and generate

the phenogram. Jaccards similarity coefficient was utilized for estimating the pairwise similarity

.

Ocimum basilicum was found to be closer to Ocimum sanctum. Interspecific study of Ocimum

sanctum further revealed that although, the tulsi samples were taken from different places they

were morphologically and genetically similar depicting that there was not much genetic

diversity.From these plants, SCAR markers can be developed in future. They are highly efficient,

produce sharp and reproducible bands, are economical than other molecular techniques.

Key Words: Molecular Markers, Authentication, Herbal Drugs, AFLP, RAPD, SCAR



PO320

THIAZOLIDINE-2, 4-DIONE & ITS ANALOGUES AS ANTICANCER MEDIATED BY

DEPLETION OF INTRACELLULAR CALCIUM: A REVIEW

Karuna S. Shukla*1, Monika

1, Shailendra Pandey

2, Pooja Chawla

3

1 School of Pharmacy, Babu Banarasi Das University, Lucknow

2 Research Lab KAPL Vasai (E) Mumbai

3 Gyani Inder Singh Institute of Professional Studies, Dehradun

[email protected]

Calcium ions plays a crucial role in key biological processes. The major intracellular calcium

store in the endoplasmic reticulum(ER). Depletion of endoplasmic reticulum calcium stores

promote endoplasmic reticulum stress and that leads to the phosphorylation of eukaryotic

initiation factor 2 (eIF2) and thereby inhibition of translation initiation. Translation initiation

plays an important role in regulation of cell proliferation and malignant transformation and is

therefore an attractive target for the development of mechanism specific anticancer drugs.

Thiazolidine-2, 4-dione & its analogues exhibit anticancer properties mediated by inhibition of

translation initiation. These compounds causes partial & sustained depletion of intracellular

calcium stores because they simultaneously release calcium stores. This partial depletion of

intracellular calcium stores causes activation of eIF2 kinases (PKR or/and PERK) leading to the

phosphorylation of the -subunit of eIF2 (eIF2 ) and inhibition of translation initiation and this

leads to the regulation of cell proliferation i.e. anticancer so it is concluded that thiazolidine-2, 4-

dione & its analogues as Anticancer mediated by depletion of intracellular Calcium.



PO321

SIMULTANEOUS ESTIMATION OF PIOGLITAZONE AND LOVASTATIN BY RP-

HPLC

Monika1*, Karuna Shanker Shukla

1, Shailendra Pandey

2, Pooja Chawla

3

1School of Pharmacy, Babu Banarasi Das University, Lucknow

2Research Lab. KALP Vasai (E) Mumbai.

3 Gyani Inder Singh Institute of Professional Studies, Dehradun

Email id: [email protected]

Diabetes is a chronic metabolic disorder that prevents the body from utilizing glucose completely

or partially. Obesity is major problem in diabetic patients. Being overweight means increased

insulin resistance. Therefore, Statins are now commonly prescribed for diabetic patients.

Pioglitazone is a thiazolidinedione derivative and it is used in patient with NIDDM. The

probable mechanism of action of Pioglitazone is through the selectively stimulation of nuclear

receptor peroxisome proliferator-activated receptor gamma (PPAR-). Whereas, Lovastatin is an

inhibitor of 3-hydroxy-3-methylglutaryl-coenzymeA reductase, an enzyme that catalyzes the

conversion of HMG-CoA to mevalonate. These drugs are therapeutically used to overcome

serious risk factors associated with cardiovascular diseases. A new simple and precise reverse

phase isocratic high performance liquid chromatography method has been developed for the

determination of Pioglitazone and Lovastatin in formulations. The separation was achieved on

millennium M Sil C18 column using acetonitrile: 20 mM NaH2PO4 (60:40 % v/v) as a mobile

phase and pH was adjusted 3.0 with 5% ortho phosphoric acid. The flow rate was kept at 1

ml/minute, the analytes were screened using UV detector at 230 nm wavelength. The retention

time of Pioglitazone and Lovastatin were found to be 4.1 and 6.5 minutes respectively.



PO322

DOUBLE PYLORUS: AN UNCOMMON FEATURE OF STOMACH

Mayank Kulshreshtha*, Manjul Pratap Singh

School of Pharmacy, Babu Banarasi Das University, Lucknow.

Double pylorus (DP) is an uncommon condition consisting of a double communication between

the stomach and the duodenum. DP can occur as either a congenital abnormality or an acquired

complication of peptic ulcer disease. It occurs more often in men (2:1), as well as with other

peptic diseases. The majority of reported cases of double pylorus is acquired and are attributed to

complications of ulcers at the antrum-pyloric area or at the duodenal bulb. Most of them are

consequences of gastric ulcer, and only few cases are due to duodenal ulcer. In this patient, the

previous history of gastric ulcer and the presence of scar tissue at the endoscopic examination

indicated that the lesion are acquired. In diabetes the lack of microcirculation can be the cause.

The majority of the patients respond well to medical treatment, regardless of whether the fistula

is open or closed. However, refractory symptoms can occur in about 20% of the patients, and

surgical treatment is necessary. With the use of potent inhibitors of acid production such as the

proton pump inhibitors, we believe that this number may be reduced. In the clinical setting,

double pylorus can present itself with epigastric pain, dyspeptic symptoms and upper

gastrointestinal bleeding or can be found incidentally.

Key Words: Double pylorus, Duodenal ulcer, Proton pump inhibitors



PO323

TRITERPENOIDS: A POTENT HEPATOPROTECTIVE AGENT

1Gunja Srivastava*,

1Manjul Pratap Singh,

2Anurag Mishra

1School of Pharmacy, Babu Banarasi Das University, Lucknow.

2Faculty of Pharmacy, Ashoka Institute of technology and Management, Varanasi.

Liver diseases are a major problem of worldwide proportions, people of nearly every age are

suffering from some or other problem related to liver. Liver damage is very common since

liver has to encounter several toxic substances during their metabolism. To counter with this

loophole there is a demand of such agents that has the capability to fight against such effects.

Due Growing interest in the elucidation of the biological functions of triterpenoids which are

ubiquitously distributed throughout the plant kingdom, they are major component of some

traditional medicinal herbs and traditionally being acclaimed as very potent hepatoprotective

agent. Plants with such constituents have been the remedy of choice for several ethnic

societies that has been subjected to hepatic ailments some or other times. In recent years it

has attracted considerable attention due to its double edged sword effect on liver defined in

terms of prevention and cures both. This review summarizes the beneficial effects of

triterpenoids and is an initiative to establish its identity as effective hepatoprotective agents

and also to explore this moiety to fullest of its extent in the field of research and development

so as to deplete this disease from the society and can be established as a boon for the

medicine industries those generating such hepatoprotective drugs.



PO324

CURCUMIN AS A DRUG IN TREATMENT OF ORAL CANCER

Shaista Suhail1*

, Kumud Nigam1, Saurabh Srivastava

2

1Department of Oral Pathology & Microbiology, King Georges Medical University, Lucknow,

UP, India

2Department of Oral & Maxillofacial Surgery, King Georges Medical University, Lucknow, UP,

India

Curcumin (diferuloyl methane), a hydrophobic polyphenol derived from the dietary spice

turmeric. It possesses properties of anti-inflammatory and anti-cancer following oral

administration. It has mechanisms of action including several cell signalling inhibition pathways,

cell cycle (cyclin D1 and cyclin E), apoptosis (activation of caspases and down-regulation of

antiapoptotic gene products), proliferation (HER-2, EGFR, and AP-1), survival (PI3K/AKT

pathway), invasion (MMP-9 and adhesion molecules), angiogenesis (VEGF), metastasis (CXCR-

4) and inflammation (NF-jB, TNF, IL-6, IL-1, COX-2, and 5-LOX), effects on cellular enzymes

such as cyclooxygenase and glutathione S-transferases, 37mmune-modulation. Curcumin also

affects gene transcription mechanism and induce apoptosis in preclinical models. The activity of

curcumin reported against leukemia and lymphoma, gastrointestinal cancers, genitourinary

cancers, breast cancer, ovarian cancer, head and neck squamous cell carcinoma, lung cancer,

melanoma, neurological cancers, oral cancer and sarcoma reflects its ability to affect multiple

targets. Epidemiological and clinical studies have suggested that cancer could be prevented or

significantly reduced by treatment with anti-oxidant and anti-inflammatory drugs, therefore,

curcumin, a principal component of turmeric (a curry spice) showing strong anti-oxidant and

anti-inflammatory activities, might be a potential candidate for the prevention and/or treatment

of oral cancer. Phenolic compound (curcumin) also play an important role as chemopreventive

agents and the development of chemopreventive strategies is an urgent priority in public health.

Cancer is one of life threatening disease of the world. Herb is considered one of the greatest

man-made medicine(drug) which can be easily available and effective. It is the cheapest method

to cure the disease or to prevent it. The herb can be use to make gels, ointments, in tooth paste

etc. for prevention of disease in future. As many technologies are available now a days, it will be

great to make use of this herbs in many ways for prevention or cure of cancer.



PO325

FORMULATION AND EVALUATION OF METOPROLOL TARTRATE

MICROSPHERES

M.V. Ramana*1, Kamal Dua

2

1 Amity University, Lucknow, U.P., India

2 School of Pharmacy and Biomedical Sciences, The University of Newcastle, Newcastle,

Callaghan NSW 2308, Australia

[email protected]

Antihypertensive drugs were currently available in the market largely in the form of

conventional dosage forms. There is a need to develop controlled release drug delivery systems

for these categories, so as to optimize the therapy and accrue the benefits enumerated with such

drug delivery systems. One such approach is using polymeric microspheres as drug carriers.

Metoprolol tartrate is rapidly absorbed from both gastric and intestinal regions, after oral

administration.In order to retard the release rate of the metoprolol tartrate, microspheres were

prepared with varying concentrations of a mixture of a water insoluble polymer, ethylcellulose

(EC) and a water-soluble polymer, polyethyleneglycol-6000 (PEG-6000); Table 1. The prepared

microspheres were evaluated for various physicochemical characteristics and in-vitro drug

release.The percent yield of microspheres was in the range 75.2 to 87.3%. A considerable

amount of materials were entrapped into the microspheres (91.0%- 95.7%). This is quite

common with the solvent evaporation method. Particle size of microspheres ranged from 3.27

m to 163.5 m and the average diameter was found to be in the range of 73.2 m to 85.5 m.

FT-IR spectral analysis and DSC concluded the absence of any interaction between the drug and

carriers. The release-time profile of metoprolol tartrate from microspheres in 0.1 N hydrochloric

acid solution was to the extent of 33.4% to 60.2%. The release of metoprolol tartrate from MPT-

3 and MPT-4, in phosphate buffer solution (pH 7.4) was complete within 8 and 7 hours

respectively, whereas incomplete release (72.3%) occurred from MPT-1. Nearly complete

release (98.5%) of metoprolol occurred from MPT-2 in 10 hours, Hence, formulation MPT-2

would be a preferred formulation. The release of metoprolol followed the zero-order kinetics, as

the R2 value was higher for zero-order (R

2 = 0.9642) than for the first-order (R

2 = 0.8260) and

the release mechanism involved is diffusion rate limited (R2 = 0.9865) from microspheres. The

prepared microspheres of metoprolol tartarte eliminate the need for multiple dosing there by



increasing patient compliance and decreasing the occurrence of adverse effects by providing a

prolonged release of drug.

PO326

Model-based Drug Development: Applicable to Oncology

Shubham Sharma*, Nimisha Mishra

ASET, Amity University Uttar Pradesh, Lucknow Campus, Lucknow

Model-based drug development (MBDD) is an approach that is used to organize the vast and

complex data streams that feed the drug development pipelines of small molecule and

biotechnology sponsors. Such data streams are ultimately reviewed by the global regulatory

community as evidence of a drugs potential to treat and/or harm patients. Some of this

information is captured in the scientific literature and prescribing compendiums forming the

basis of how new and existing agents will ultimately be administered and further evaluated in the

broader patient community. As this data stream evolves, the details of data qualification, the

assumptions and/or critical decisions based on these data are lost under conventional drug

development paradigms. MBDD relies on the construction of quantitative relationships to

connect data from discrete experiments conducted along the drug development pathway. These

relationships are then used to ask questions relevant at critical development stages, hopefully,

with the understanding that the various scenarios explored represent a path to optimal decision

making. As MBDD becomes more integrated into the pharmaceutical research community, a

more rational explanation for decisions regarding the development of new oncology agents as

well as the proposed treatment regimens that incorporate both new and existing agents can be

expected. Hopefully, the end result is a more focussed clinical development programme, which

ultimately provides a means to optimize individual patient care.

Keywords: clinical pharmacology, model-based drug development, pharmacodynamics,

pharmacokinetics.



PO327

EFFECT OF BUXUS WALLICHIANA BAILL EXTRACT AGAINST EXPERIMENTAL

INDUCED AMNESIA IN SWISS ALBINO MICE

Satyendra K Rajput*, Varshala Pal, Arun K Sharma, Shyam Sundar Agrawal

Department of Pharmacology, Amity Institute of Pharmacy, Amity University, Uttar Pradesh,

Sector - 125, Noida, 201303, India.


The present study was undertaken to investigate the effect of Buxus wallichiana Baill (wood), on

scopolamine-induced amnesia in mice. Amnesia is the bench mark of Alzheimers diseases

(AD) which would become life threatening at sometime. Male mice were randomized to receive

different dose (125, 250, and 500 mg/kg, p.o) of methanol extract of Buxus wallichiana (MEBW)

for fifteen day against donepezil as reference standard. The Effect on learning and memory was

done using standard memory test includes elevated plus maze (EPM), morris water maze

(MWM), passive avoidance task (PAT). MEBW (125, 250 and 500 mg/kg, p.o) has shown

substantial lessen in transfer latency (TL) in EPM whereas, a significant increase has observed in

step down latency in PAT as compared to scopolamine (1 mg/kg, i.p) treated group. Moreover,

MWM test reveal the mark reduction in escape latency time during training by employing

MEBW (125, 250, 500 mg/kg, p.o). In Probe trial, these animals had spent more time compared

to scopolamine treated group. However these drugs did not alter locomotor activity. It has been

concluded that beneficial effect of MEBW on mice may be attributed to the facilitation of

cholinergic transmission and thereby improvement in memory. Therefore, it would be

worthwhile to explore the therapeutic potential of Buxus wallichiana in the management of

patients with cognitive disorders.



PO328

Formulation development & Characterization of Ethosomal gel Approved for the

Treatment of Arthritis

Pratibha Gupta*, Anupum Kumar Sachan

DYANAND DINANATH COLLEGE OF PHRMACY NH# 86, HAMIPUR ROAD, KANPUR-

20914

[email protected]

The present research has been under taken with the aim to develop topical ethosomal gel of

diclofenac by using such as carbopol ,propylene glycol, phospholipid, and soya phosphatidly

choline in different concentration .The oral use of diclofenac is not much recommended as it has

many side effect, thus this gel formulation is made for better patient compliance and reduced the

dose of drug and avoid the side effect like liver& a kidney damage. The different preformulation

studies i.e. Infrared and organoleptic study conforms its purity high moleculer weight ,water

soluble polymer of soya phosphatidly choline ,carbopol 940,phospholipid that possess very high

viscosity ,transparence film forming property at low concentration, gel formulation were

characterized for pH determination ,spreadability & drug release ,drug content ,viscosity

measurement and in vitro drug release .From the study, it was concluded the diclofenac gel

containing carbopol 940 showed good consistencey ,homogencity , spredability & drug release.it

given as wider prospect for the topical preparation .The gel preparation shows excellent

percutaneouse absorption of diclofenac and good characterization.

Key woards: diclofenac, carbopol 940, propylene glycol, drug release.



PO329

Nano Particles in Drugs

Mridul Rajeev, Nikhar Shukla, Monika Kamboj, Richa Khare

Amity School of Applied Sciences, Amity University, Lucknow.

Nano particles are very effective in comparison to other one because they are small in size and

can be used anywhere for drug delivery. The main objective of nanoparticles is to control and

manipulate biomacromolecular constructs and supramolecular assemblies that are critical to

living cells in order to improve the quality of human health. By definition, these constructs and

assemblies are nanoscale and include entities such as drugs, proteins, viruses, cellular lipid

bilayers, cellular receptor sites and antibody variable regions critical for immunology and are

involved in events of nanoscale proportions. Therefore nanotherapeutics is the only method

which will allow a deeper understanding of human longevity and human ills that include cancer,

cardiovascular disease and genetic disorders. This is the only technique which provides a wide

range of manmade nanostructures that may be controlled as a function of size, shape and surface

chemistry.

Key words: Biomacromolecular, supramolecular, nanotherapeutics and nanostructures



PO330

Role of Biotechnology in Pharmaceuticals

Nikhar Shukla*, Mridul Rajeev, Smriti Khare and Richa Khare

Amity School of Applied Sciences, Amity University, Lucknow

For hundreds of years humankind has used biotechnology in agriculture, food production,

and medicine. Depending on the tools and applications it often overlaps with the fields

of bioengineering, biomedical engineering, bio manufacturing, etc. In recent years, the number

of drugs of biotechnological origin available for many different diseases has increased

exponentially, including different types of cancer, infectious diseases, cardiovascular,

neurological, respiratory and autoimmune diseases. The pharmaceutical industry has used

different technologies to obtain new and promising active ingredients as exemplified by the

fermentation technique, recombinant DNA technique and the hybridoma technique. The

pharmaceutical industry in their attempts to discover new molecules has found annually in the

biotechnology industry with exponential growths. In this paper we reviewed the most important

biopharmaceuticals such as blood factors, hormones, enzymes and vaccines.

Key words: bioengineering, biomedical engineering, bio-manufacturing, Biopharmaceuticals

and hybridoma technique.



PO331

Impact of chitosan nanoparticle in drug delivery

Upasana Yadav*

Amity School of Applied Sciences, Amity University, Lucknow-226017, U.P., India

E. Mail:[email protected]

The application of nanotechnology for the treatment, diagnosis, monitoring, and control of

biological systems has recently been determined by the National of Health (NIH) as

nanomedicine. The strategy of Nanoparticle delivery plays a significant impact on global

Pharmaceutical planning and marketing. Polymeric nanoparticles are used to control the drug

release, to improve the dissolution of poorly soluble drugs in addition to improve the

bioavailability of degradable substances such as protein. They also enhance the uptake of

hydrophilic substances across the epithelial layers and have the potential for intracellular drug

delivery. The submicron size range of nanoparticles is not only suitable for parenteral application

but also applicable for mucosal routes of administration, i.e., oral, nasal, and ocular mucosa

which are non-invasive route. Thus nanoparticle formulations are more advantageous over

traditional dosage forms. The main aim of the present review deals with the nanoparticles of

chitosan, which is a natural and bio-degradable polymer. The review focuses on the isolation,

purification, characteristic features derivatives of chitosan, preparation techniques, evaluation

methods and applications of chitosan nanoparticles.

Keywords: chitosan, nanoparticles, bioavailability, biodegradable polymer.



PO332

CHEMICAL CONSTITUENTS AND PARTS OF PLANTS AS

IMMUNOMODULATORS

Kalpana Sonwani*, Ashutosh Kumar Yadav

Department of Pharmacology , School of Pharmacy , BBD University , Lucknow

[email protected]

Immunomodulators (IM) are the drugs affecting the immune system. They are used when the

immune response is impaired. Hence to maintain a Disease Free State, modulation of immune

response by either its stimulation or suppression, can be a helpful therapy . Immunodeficiencies

occurs when one or more of the components the immune system are inactive. It included

autoimmunity, hypersensitivity and HIV etc. IM include corticosteroids, cytotoxic agents,

thymosin and immunoglobulins etc. The present review is focused Immunomodulatory effects

of some medicinal plants based on the chemical constituents such as Flavanoids , Alkaloids ,

Phenolic compound , Steroids ,Terpenoids and Tannins present in that particular plant such as

Ficus carica (leaves) ,Aloe vera (Leaves), Cleome gynandra (Aerial parts), Allium sativum

(Whole plant) , Asparagus racemosus ( Root), Tinospora cordifolia (Stem), Ocimum sanctum

(Whole plant) ,Curcuma longa (Balb), Mangifera indica (Stem bark) etc. had been discussed

which are previously explored by the various researchers for their immunomodulatory

activity. As discussed above, it can be conclude that there is a wide scope in herbal drugs and

their formulations in the treatment of immune disorders.

Keywords: Immunomodulators, HIV, Immunodeficiency



PO333

CLINICAL BENEFITS OFHERBAL BASED ADAPTOGENS

Pooja Rao*, Dharamveer

Department of Pharmacology, School of Pharmacy, BBD University, Lucknow

Email id:-poojaraoaryan [email protected]

Plant adaptogens are compounds that increase the ability of an organism to adapt to

environmental factorsand to avoid damage from such factors. The beneficial effects of multi dose

administration of adaptogens are mainly associated with the hypothalamicpituitary adrenal

(HPA) axis.Thesingle dose application of adaptogensis important in situations that require a

rapid response to tension or to a stressful situation.In this case, the effects of the adaptogens are

associated with another part of the stresssystem, namely, the sympathoadrenal-system (SAS),

that provides a rapid response mechanism mainly to control the acute reaction of the organism to

a stressor.Theadaptogens like Rhodiolarosea, Schizandrachinensis, Eleutherococcussenticosus

etc showed stimulating effects.The phenolic compounds include phenylpropanoids and

phenylethane derivatives such as salidroside (rhodioloside), rosavin, and lignans are structurally

similar to the catecholaminesthe mediators of the sympatho-adrenal- system (SAS)Recent

pharmacological studies of some adaptogens give a rationale to their effects at the molecular

level. Adaptogens may be regarded as a novel pharmacological category of anti-fatigue agents

that perform the several functions including induce increased attention and endurance in

situations of decreased performance caused by fatigue and/or sensation of weakness and reduce

stress-induced impairments and disorders related to the function of stress (neuro-endocrine and

immune) systems.



PO334

ANIMAL MODELS FOR THE STUDY OF STRESS VULNERABILITY RESILIENCE

Richa Mishra*, Ashutosh Kumar Yadav

Department of Pharmacology, School of Pharmacy, BBDUniversity, Lucknow

[email protected]

Resilience is defined as the adaptive maintenance of normal physiology, development and

beha

national conference on drug discovery & formulation development 2016 by amity abstract cd.pdf

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