The Evolution of Cell Culturingfor Mid- up to Large-Scale Batches

NANT XL System

NANT XL Cartridge

Cell Culture Flask 1175 cm2

Cell Culture Flask 23180 cm2

Aseptic Connectorsand Disconnector

HarvestingBag1 L

Waste Bag4 L

Buffer Bag2 L

AuxiliaryBag 1

100 mL

AuxiliaryBag 21 L

Media Bag3 L

A success story: components you already know, engineered in an easy-to-load, disposable, closed system for safe and cGMP-compliant cell processing.

NANT XL Bioreactor

IntegratedImagingSystem

CO2percentagecontrol

RemovableTray

Two SeparatedCell CultureContainers

Advanced2-axis TiltingPrecise

VolumesManagement

Full automation, built-in microscope with confluency estimation, remote continuous monitoring: the key features for a successful and traceable cell production. Sophysticated technology made simple

thanks to the distinctive features of NANT Automated Cell Culturing Systems.

Aseptic Connections, in seconds

As in all NANT Automated Cell Culturing Systems, the different sections of NANT XL Cartridge are designed to be quickly and

easily connected and disconnected - when required - thanks to the

integration of validated aseptic connectors and disconnectors.

Ready for Downstream Processing

NANT XL System output is a cell suspension in a bag, ready for

virtually any kind of required downstream processing operations,

including those that require welding to other reagents-containing

bags too.

Unique Microscopic Imaging and

Cell Confluency EstimationThe built-in auto-focus microscopic imaging automatically

acquires and calculates Cell Confluency. The ideal feature to identify the optimal timeframe for cell harvesting - or just to see

cells growing in real-time.

Smart Process Monitoring

All key process parameters as Temperature, CO2 mixture

percentage and Cell Confluency always displayed on user interface. An intuitive, smart depicting of the current protocol

phase: everything at your fingertips.

Remote Monitoring

Special Web Application allows full, real-time remote monitoring

of in-process control information. No expensive and

time-consuming personnel access to the cell room required.

Subculturing, automatically

Automated subculturing from an initial 175 cm2 flask to a subsequent 3180 cm2, 5-layered container. A competitive feature

allowing to obtain large amounts of expanded cells even from

small quantities of starting material.

175 cm2 3180 cm2

Highlights

• Automated and flexible cell expansion system with an integrated sub-culturing process, for mid- up to large-scale batches.• Remote monitoring of culture conditions and in-process control.

• Consistent results in terms of yield, viability and quality of expanded MSCs.

• Flexibility of the system for different types of cell cultures, both in adhesion and suspension.• Full traceability of operations, disposables and identification of users, with final cell culture report downloadable as PDF.

Amount of starting material

Days of cultureCell Culture

Surface (cm2)

No. of cells harvested

Density of harvested cells

(no. of cells/cm2)

Viability of harvested cells

(%)

1x106

pre-expanded MSCs

8 ± 1175 cm2

to 3180 cm2

5 layers flask2.72 x 108

± 2.55 x 1078.56 x 104

± 8.00 x 10396.0

Representative images of MSCs taken by the NANT XL System during cell culture

0

20

40

60

80

100

CD45 CD14 CD31

% Positive markers expression % Negative markers expression

0

20

40

60

80

100

CD73 CD90 CD166 CD105

Example of Cell Expansion with the NANT XL System

ORDERING DETAILS

ItemNANT XL BioreactorNANT XL Cartridge

ENQUIRIES AND SERVICE

VivaBioCell S.p.A.

Via del Cotonificio, 127 - 33100 Udine, Italy+39 0432 54 56 74info@vivabiocell.it

Catalogue NumberNXB-01NXC-01

Technical Specifications

35 – 55 mL (175cm2)650 - 2000 mL (3180 cm2)Up to 3 LUp to 2 LUp to 100 mLUp to 1 LUp to 1 LUp to 4 L

PolystyreneEVA, EVOH Tygon® and SiliconePharmed® BPTPTFE and PES

>25 kGyUnidirectional sampling valvesGenderless sterile-septumconnectors and disconnector

Working volumes

Cell Culture Flask 1Cell Culture Flask 2

Media Bag

Buffer BagAuxiliary Bag 1Auxiliary Bag 2Harvesting Bag

Waste Bag

Materials

Cell Culture FlaskBags (UV-safe)

Tubing (fluid path)Tubing (pump segment)

Gas Filters (0,22 µm)

Other info

β-rays Irradiation DoseSampling Ports

Sections Connections

71 x 72 x 79 cm28,0 x 28,3 x 31,1 in

64 kg141,1 lbs

15-30°C59-86°F

1-120 mL/min

7” resistive touch-screen

Wireless remote process controland ethernet port

Barcode-based User andCartridge identification

Web application-based access tokey process parameters andmicroscopic images

Automated recording ofuser-dependent operations,key process parameters (temperature,% CO2, cell confluency), warning log,sampling log.

100-240 VAC, 50-60 Hz

Dimensions(W x D x H)

Weight

Operating

Temperature

Pump Flow Rate

User Interface

Connectivity

Traceability

Remote Access

Cell Culture Report

Information

Power Supply

NANT XL Bioreactor NANT XL Cartridge

NANT XL System is designed for Research purposes and any use for human therapy must be conducted under the full responsibility of the user.

VivaBioCell S.p.A. is part of NantCell, Inc., a subsidiary of NantWorks, LLC.www.vivabiocell.it

vivabiocell.it/nantxl

VBC-

MP-

006_

v1