Myeloid cells matter

Conclusions

Glut1+ CD14++ CD16+

Associated with inflammatory markers

HIV

Immune activationHIV-related diseases

Joshua Anzigen:

Intermediate monocytes are possible markers to identify HIV-infected subjects at risk for HIV-1 related co-morbidities

Francesca Graziano:

Purging the HIV reservoir

?

ATP stimulation of Mφ

Adapted from M. Stevenson, Nat Med. 2003

ATP

HAART

Extracellular virion release

Intracellular virions accumulation

Maria Isabel Sade-Ovalle:

Tim3-Gal9T cell-Mφ

interaction

Favor HIV entry

Favor HIV-specific

T cell responses

Blocking antibodies

modulation of cytokines

release from T cells and Mφ

Control of M.tb. growth

in HIV patients

?

Modulation of the Tim3-Gal9 pathway favor antimicrobial immunity against Mycobacterium tuberculosis

Tran Huyen:

In TB – HIV coinfection

Alteration of pro/anti inflammatory genes and modulation of the

complement system in monocytes

Identify specific signatures prognostic for

development of TB-IRIS

Monocytes contribute to the development of TB-IRIS

Dysregulation of Metabolic pathways

Non-communicable diseases

Reservoir of HIV

HIV-related dementia

Opportunistic infections

IRIS

Myeloid cells matter

Points for discussion• Immune activation drives disease progression.

• Classical (CD14+CD16-) vs non classical (CD14+/CD16+) monocytes: what is their role in immune activation?

Increased level of blood non classical monocytes is correlated with disease progression. Non classical M-DC8+ monocytes accumulate in the peripheral blood of viremic untreated patients (Dutertre et al. 2013).

• Better definition of monocyte/macrophages and DC subsets in several anatomical compartment…and their role as viral reservoirs.

• Focus on alternative approaches to purge HIV reservoirs.