mucorimietiana sp. nov. - abapabap.co.in/sites/default/files/paper-11_22.pdfthe specific epithet...

Current Trends in Biotechnology and PharmacyVol. 11 (1) 99-102 January 2017, ISSN 0973-8916 (Print), 2230-7303 (Online)

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AbstractA thermophilic, heterothallic species of Mucor

- M. imietiana A. Swathi Sri.& A.Subrahm.sp.nov.isolated from garden soil from MIET campusis described and illustrated. It grows well at 500

C on synthetic mucor agar and potato-dextroseagar from pH 3.0-9.0 with an optimum pH at 7.0.The specific epithet refers to its habitat.

Key words: Mucorimietiana sp. nov.,Thermophilic, Heterothallic, Garden soil MIETMeerut

IntroductionDuring study on fungal flora of Meerut district

a novel species of Mucoroptimally growing at 500

C was isolated from MIET garden soil. Itsmorphology and culture characters observed onmonosporic culture grown on synthetic mucoragar at 500C are presented.

MucorimietianaA. Swathi Sri.& A. Subrahm.sp.nov. (Fig 1-6; Plate 1-5).

Colonies on synthetic mucor agar at 500 Cfast growing cover 80 mm plate in 48 hrs. Whiteat first, slowly turn mouse gray with age andsporulation; turf 2mm in height; sporulationabundant; reverse colony white with black areashere and there, heterothallic.

Mycelium hyaline, smooth, aseptateandbranched; sporangiophoreshyaline occasionallyswollen at the base, one septate belowsporangium and branched; branching sympodialto irregular, more or less umbellate at the distalend. Occasionally compound umbels are also

present or one of the branches ofsporangiophores may become sterile spine.Sporangia spherical,multispored, hyaline whenyoung, pale golden yellow when mature, 14.0-48.0µm, columellate;columella variable in shape,globose to ellipsoid, collarate 17.0-34.0 X 14.0-28 µm; sporangial wall smooth, hyaline, distalpart of the sporangial wall becomes deliquescentat maturity to release sporangiospores;sporangiospores globose, small, hyaline, onecelled, smooth 2.0 – 3.0 µm often agglutinated.Rhizoidsabsent; Zygospores absent even afterprolonged incubation at lower temperatures like5-20°C.

Habitat : Garden soil, MIET campus, Meerut,Uttar Pradesh, India.Temperature relations :Optimum-500 C; Minimum 280 C; Maximum- 600

CpH relations : Grows from pH 3.0 -

9.0; Optimum pH 7.0Date of isolation : 22 October, 2009Culture deposited at : NFCCI2251 (Agarkar

Institute), Pune, Indiaand MTCC,Chandigarh- 10744

Mucorimietiana sp. nov (Fig 1-6; Plate 1-5)Sporangiophores stipes, stipes sympodial

utincompositemagisvel minor umbellateproculcolus terminus sporangia globosecolumellate , teres , rutilus crocus , parietisterestenuis deliquiscent 14.0 – 48.0 µm columellavariable in vultus , teresglobose ut ellipsoidcollarate 17.0 – 34.0 X 14.0 – 28.0 µm. Rhizoids

Mucorimietiana sp. nov.

A. Swathi Sri and A. Subrahmanyam1*Department of Biotechnology, Krishna University, Machilipatnam, AP, India

1Department Biotechnology, Meerut Institute of Engineering & Technology (MIET),NH-58 Bypass crossing, Meerut -250005, U.P., India*For Correspondence - [email protected]



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adversusutsporangiophoresnunquamtendo.Sporangiosporesvegrandissmooth,globose,unus celled 2.0 - 3.0µm zygospores nonproduced.Habitat: Ortus Terra MIET CastraMeerut.

DiscussionTheromophilism in fungi was first reported

in Mucorpusillus(6). Since then few morethermophillic species of Mucor were reported (1-3, 5). But Schipper (4) transferred all thermophilic

Figs. 1-6. Mucorimietiana sp. nov

1. Umbellate arrangement sporangoiphores 400X2. Compound umbel 400X3. One of the branches of sporangoiphores modified into a sterile spine 400X4. Mature sporangium 400X4a. Different shapes of columella400X5. Mature sporaingium after dissolution of sporangial wall 400X6. Sporangiospores1500X

Swathi Sri and Subrahmanyam


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Plates 1-5.Mucorimietiana sp. nov.

1 & 2 Umbellate arrangement of sporangoiphores X2003 Compound umbel X2004 Mature sporangium after dissolution of sporangial wall X2005 SporangiosporesX400

Mucor species developing rhizoids and stolensto Rhizomucor.

Looking at the sympodial branching of thesporangiophores which appear more or lessumbellate at the distal end, thermophilic nature,heterothallic behavior of the present isolate onemay consider it as Rhizomucorpusillus.

But the present isolate althoughthermophilic and heterothallic never producesrhizoids and stolanes. Therefore it is consideredas species of Mucor rather than Rhizomucor.Other differences are presented in Table-1.

The present isolate is Mucor like andresembles the mesophilic species M. hemilis in



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Table 1. Comparative characters of M. imietiana and M. hemilis

Character M. imietiana M. hemilis

Temperature Thermophilic Mesophilic

Rhizoids &Stolons Absent Absent

Sporangiophores Branching sympodial to irregular,upper part umbellate,colorless Sympodial, colorless

Compoundumbels Occasionally present Absent

Sporangia Spherical golden yellow 14.0 - Yellow to dark brown up to48.0 µm in diameter 70 µm, in diam.

Sporangial wall Smooth golden yellow deliquescent Deliquescent at maturityat maturity

Columella Globose to ellipsoidal 17.0-34.0 X Globose 38.0-30.0 µm14.0-28.0 µm

Sporangiospores Globose, hyaline 2.0-3.0 µm Ellipsoidal flat at one end5.5-8.5 X 2.5-5.5 µm

Zygospores Heterothallic Homothallic

characters like sympodial branching ofsporangrophores, globose to ellipsoid, hyaline,smooth columella andsporangiospores. Howeverthe present isolate appears distinct in beingthermophilic, smaller dimensions of sporangia,columella, sporangiospores and absence ofoidea. Occasionalpresence of compoundumbels,modification of one of the branches ofsporangiophores into sterile spine, are theadditional features of distinction. Therefore it isdescribed as new species under the nameM.imietiana. The specific epithet refers to itshabitat.

AcknowledgementsThanks are due to P.V. Brahmachari, Head,

Biotechnology and Prof.M.V. Basaveswara Rao,Director, for their interest and encouragement forcarrying out this work. Thanks are also due toShree Vishnu Saran, Chairman, MIET, Lt. Gen.J. M.Garga, D.G. MIET; Dr. V.N. Sharma, DirectorBiotechnology for Laboratory facilities,ShriSudheerKumar Bhatt for typing script andShri Shariq Iqbal, Lab Supervisor forphotography.

References1. Cooney, D.G. and Emersom, R. (1964).

Thermophilic fungi : An account of theirbiology, actissvities and classification. Publ.Free man& Co. San Francisco & London.

2. Mirza, J.H., Khan, S.M., Begam, S. andShagufta, S.(1979). Mucorales of Pakistan.University of agriculture, Faisalabad,Pakistan 183P.

3. Qureshi, M.S.A and Mirza, J.H.(1983).Rhizomucorpakistanicus Qureshi &Mirza sp. nov.Biologia, Lahore 29: 343-344.

4. Schipper, M.A.A. (1978). On the generaRhizomucor and Parasitella stud. Mycol. 17:53-71.

5. Subrahmanyam, A. (1981). Studies onthermomycology: Mucorthermohyalospora.Bibliotheca mycologia. 91:421-423.

6. Tsiklinsky, P. (1899).Sor les mucidinesthermophiles. Ann. Inst. Pastuer. 13: 500-505

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