monitoring your wetland - macroinvertebratesclean-water.uwex.edu/pubs/pdf/macroinvertebrates.pdf ·...
TRANSCRIPT
a primer to site-level monitoring activities for volunteer coordinators
This publication is part of a Monitoring Your Wetland series available online in pdf format at:wetlandmonitoring.uwex.edu
yourMonitoring
Wetland
. . . . . . . . . .Macroinvertebrates
Macroinvertebrates may be small and spineless, but when it comes to the cycling of wetland nutrients there’s
nary a niche where you won’t find them. Some, like snails, graze peacefully on aquatic plants, while others, like dragonfly nymphs, voraciously prey on other invertebrates. Sowbugs, caddisfly larvae, clams and other janitorial macroinver
tebrates recycle decomposing and particulate matter. Finally, all macroinvertebrates provide food for other wetland animals.
Most macroinvertebrates are too small to noticeably stir the water’s surface, but are large enough to see with the naked eye. They include
small worms, mollusks, leeches, crustaceans, insects and insect larvae. Besides playing a star role in many important wetland functions, macroinvertebrates are sometimes employed as a tool for characterizing aquatic habitats and learning about aquatic habitat water quality. Macroinvertebrates are sensitive to physical, chemical and biological environmental stressors. And because of their limited mobility, they cannot easily avoid pollution, low oxygen levels or other unfavorable conditions.
EXPERTIS
E
RES
OURCES
HIGHHIGH
LOWLOW
About Macroinvertebrate Surveys
Macroinvertebrate monitoring can help characterize wetlands, but as a water
quality indicator, wetland macroinvertebrate monitoring has not yet matured. In other aquatic habitats, and particularly streams, macroinvertebrate monitoring has a long history as a tool for assessing water quality. Volunteers who monitor macroinvertebrates in streams look for the presence, absence or dominance of sensitive or tolerant species and then use a biotic index of health to interpret what they indicate about the quality of the stream.
Unfortunately, macroinvertebrate monitoring in wetlands is much more challenging than in streams. The macroinvertebrate community found in a wetland will differ considerably from
the communities in other wetland types. And, because there is a great deal of variation among wetland types, it is difficult to standardize protocol for collecting repre sen tative macroinver
tebrate samples or identifying a healthy or unhealthy macro invertebrate community.
Consequently, it is also difficult to determine when variations in sampled macroinvertebrate
Snail
Crayfish
Bo
b K
ort
h
About Macroinvertebrate Surveys – continued from front page communities are due to wetland degradation or natural wetland characteristics.
Differences in wetland characteristics that have nothing to do with degradation can influence the presence or absence of various species of macroinvertebrates. In contrast to shallow, landlocked wetlands, for example, wetlands connected to lakes or rivers typically have fish, which tend to reduce macroinverte brate abundance and species diversity. Additionally, naturally high levels of acidity in bogs can also reduce macroinvertebrate diver sity, but don’t necessarily indicate poor wetland quality.
Macroinvertebrate monitoring is one of the more difficult and costly monitoring efforts a wetland volunteer monitoring group can engage in. Wetland monitoring groups often become frustrated trying to achieve satisfactory results if they are determined to develop a complete inventory of wetland macroinvertebrate species or monitor macroinvertebrates as an indicator of wetland quality. When trying to monitor macroinvertebrates as indicators, sampling, picking and especially specimen identification can be difficult.
All of this having been said, macroinvertebrate monitoring may be a great activity to
engage your volunteers in if your primary goals are to raise awareness and characterize the wetland. Macroinvertebrate monitoring is a good way to teach volunteers about wetland ecology. And identifying some of the types of macroinvertebrates living in your wetland contributes
to what you know about it. This publication outlines some basic macroinverte brate survey methods that can be applied to a broad range of wetland types.
These are methods designed to help maximize volunteers’ ability to produce an inventory of species that will help characterize a wetland. We will not explain
what sampling results indicate about wetland health, since methods for doing so are nascent even for professional biologists. But for those of you interested in experimenting with preliminary methods for mon itoring macroinvertebrates as wetland health indicators, we will briefly describe and provide references to projects testing such methods in Midwestern wetland areas (see Informational Resources on the back page).
Macroinvertebrate monitoring may be a great activity to engage your volunteers in if your primary goals are to raise awareness and characterize the wetland.”
“
Wetlands alongside a lake Bog wetland Wetland with ponds
Jeff
rey
J. St
robe
l pho
tos
Monitoring Your Wetland – Benthic Macroinvertebrates
2
Costs for the equipment, supplies
and possibly laboratory services
needed to support macroinverte
brate monitoring can range from
moderate to high depending on
the rigor of the identification
standards used. Monitoring will,
at minimum, require equipment
for collecting samples. Depending
on how you structure your volun
teer group’s monitoring activities,
they may also need equipment
and supplies for separating
specimens from organic matter,
identification and preserving
specimens.
For Collecting Samples
Volunteers need at least three
basic pieces of equipment to
gather macroinvertebrate
samples. First, they need D-frame macroinvertebrate sampling dip nets and basins or pails for
holding samples. Recommended
mesh sizes for the sampling
nets ranges from 6001,200
microns. Finer mesh nets will
capture more macroinvertebrates,
but will also clog easily and can
collect an unnecessarily large,
debrisladen sample. Finally,
because volunteers need to leave
dry land to collect samples, they
will also need hip boots or waders and possibly a canoe.
For Picking Specimens
When volunteers sample macro
invertebrates from a wetland
they will almost definitely gather
a great deal of organic matter
and need to pick macroinverte
brate specimens from a mat of
duckweed, plant fragments and
muck. White invertebrate counting trays made out of fiber
glass or plastic will help volunteers
sort through the mess. Trays
used to hold collect ed samples
while volunteers pick specimens
out of organic matter should be
lightweight, flatbottomed and
shouldn’t twist or easily spill.
Macroinvertebrate picking trays
can come in a variety of sizes, but
a typical tray might measure 25
cm wide, by 30 cm long and 5 cm
deep. Some volunteer groups use
dish pans, which are affordable
and easy to find.
To assist in separating organic
matter from macroinvertebrate
specimens you may also want to
provide each volunteer with a
framed screen. The screen
frame can be made out of wood
and the screen can be made out
of chicken wire, hardware cloth
or other wire mesh with holes big
enough for large macroinverte
brates to fall through, but small
enough to hold back a large
quantity of vegetative matter.
The screen frame should just fit
within the perimeter of a tray and
suspend the screen below the lip
of the tray and above the tray
bottom. You will also need tools
for grabbing and removing
specimens from organic matter,
such as forceps and eyedroppers.
Volunteers may prefer different
tools for picking and you may
want to experiment with a
number of tools, such as plastic spoons, basting brushes or
turkey basters.
For Identification and Preserving
After picking, you will need
additional supplies and possibly
laboratory services to complete
the identification process. For
rudimentary identification that
can be conducted in the field,
volunteers will need a few
basic supplies including
macroinvertebrate taxonomic keys, ice cube trays for sorting
picked macroinvertebrates into
families, hand-held magnifying glasses and data recording sheets. For more rigorous
identification, volunteers will
need access to a laboratory supplied with microscopes.
If they are not identifying in the
field, they will also need supplies
for preserving specimens
including vials or bottles to hold
macroinvertebrates and a
preservative, such as alcohol or
glycerin. To obtain the most
rigorous identification of sample
specimens, volunteer groups will
need professional laboratory
services. Unfortunately,
laboratory services can cost
hundreds of dollars and often
exceed the resources available
to many volunteer monitoring
groups.
Supplies and Equipment
Monitoring Your Wetland – Benthic Macroinvertebrates
3
Volunteers empty a D-frame sampling dip net into containers.
A wetland macroinvertebrate monitoring team will require a highly skilled team
leader and moderatetohigh skill levels among other volunteers, depending on the design of the monitoring effort. Three tasks must be completed to determine the types and species of macroinvertebrates present in a wetland. Samples must be collected, macro invertebrates must be picked from the organic matter mixed in the sample and the macroinvertebrates must be identified.
Samples must be collected using consistent methods, but volunteers can learn these methods
Survey Participants relatively easily when taught by an experienced instructor. As little as two to three hours of field training may be sufficient to train volunteers to collect samples. However, picking, and particularly identifying macroinvertebrates, can be very difficult and requires a high level of skill that cannot be taught over a short period of time.
If you ask volunteers to pick and identify specimens, you will need a highly experienced team leader to ensure volunteers are properly picking and to double check identifications. Generally, volunteers are said to struggle with identifying down to the family level.
“As little as two to three hours of field training may be sufficient to train volunteers to collect samples. However, picking, and particularly identifying macroinvertebrates, can be very difficult and requires a high level of skill that cannot be taught over a short period of time.”
Monitoring Your Wetland – Benthic Macroinvertebrates
4
To get the most representative macroinvertebrate sample possible, volunteers
need to sample at the right time and in the right locations. Volunteers don’t need to wake up before dawn or limit monitoring to days with low wind speeds, but they do need to take samples at the right time of year. The best time to sample macroinvertebrates is roughly from early June through July or midAugust. Samples collected earlier could contain large numbers of underdeveloped organ isms, which will be difficult to identify. Sampling is typically completed before August, since by late summer the macroinvertebrate community is more likely to include macroinvertebrates that have migrated in from connected water bodies and will be less representative of the wetland. Also, in late summer many wetlands can become dry.
Volunteers can determine where to sample by examining wetland vegetative composition and water depths. Volunteers should sample macroinvertebrates from both emergent herbaceous plant communities (the underwater portion) and submerged and floating aquatic communities when both are present. They should also take samples that represent a range of water
Surveying – When, Where & How to Sample depths. Depending on how dense the vegetation is, volunteers should sample in water depths ranging from no deeper than hip level down to ankle level. In deep water volunteers may find sowbugs, midges and worms, but macroinvertebrate diversity will be low compared with shallow water areas surrounded by emergent vegetation.
When taking a sample, volunteers should sweep the net through the entire water column with a vigorous jabbing motion, starting near the sediment surface. With each jab of the net, the volunteer reaches outward and then rapidly pulls toward their body with the net, dislodging macroin vertebrates
from emergent and floating vegetation. When a volunteer reaches the water’s surface with their sample, they should deposit it in a pail. To ensure the pail contains a robust collection of macro invertebrates, the volunteer should dipnet one to two more additional samples in the same general area and combine them in the
pail with the first. These two to three samples will be treated as one sample when picking and identifying.
Depending on how dense the vegetation is, volunteers should sample in water depths ranging from no deeper than hip level down to ankle level.”
“
D-frame dip net, macroinvertebrate key, containers and tools.
Monitoring Your Wetland – Benthic Macroinvertebrates
5
Unless you send unpicked samples to a lab for identification, volunteers will need to
learn how to pick macroinvertebrates from the organic matter scooped up in their dip nets. Even if your volunteers will be sending sampled specimens to a lab for professional identification, your group will likely want to pick the samples first since laboratory fees for unpicked samples are very high.
Volunteers should process samples right away while the macroinvertebrates are still moving and easy to see. To pick, volunteers should spread their sample over screens placed inside white picking trays. The macroinvertebrates will squirm down toward the bottom of the tray to escape the light and fall through the screen into the tray. Volunteers should let the sample sit for about five to ten minutes while the macroinvertebrates move down.
Physical features examined to identify macroinvertebrates include overall body
shape, number and location of legs, presence and location of gills and tails, the shape of the head capsule and unusual appendages. Macroinvertebrate casings and mode of locomotion can also be used to identify macroinvertebrates. Some crawl, swim side to side or swim updown. And among
Surveying – Picking & Preserving
Identification
After allowing the sample to settle for five to ten minutes, the screen and organic matter caught by it can be removed. Before removing the screen, volunteers should inspect it for invertebrates that did not move down into the tray. After removing the tray, volunteers can begin picking. Volunteers should continue to pick either until they have picked 150 specimens from their sample or have been picking for 30 minutes. To avoid picking bias, volunteers should resist the urge to ignore macroinvertebrates that are difficult to pick because they are fast or very small. Volunteers should pick big, small, slow and fast macroinvertebrates. Volunteers should use tweezers or an eye dropper to grab specimens, then drop them into a jar or bottle of water for temporary storage until they can be sorted and/or preserved.
those that have casings, some have a case of sticks and others a case of stone. Even with all
of these possible distinguishing features, macroinvertebrate identification is incredibly difficult.
Unless your volunteers are retired entomologists, they will probably only be able to identify most macroinvertebrates down to the order or family taxonomic levels. Nonexpert entomologists will Sorted macroinvertebrates and identification notes.
Monitoring Your Wetland – Benthic Macroinvertebrates
6
Tubifex worm
The Key to Life in the Wetlands flow chart
MACROINVERTEBRATES
Scud or sideswimmer Black fly larva Mayfly
typically not be able to identify macroinvertebrates down to the species or even the genus level. If it is important to you to have a large proportion of sampled macroinvertebrates identified down to the genus or species level and you have substantial resources to support the monitoring project, you might consider sending samples to a professional lab for identification.
Laboratories in Stevens Point and at UWSuperior offer identification services. A monitoring project can expect to pay approximately $150 in laboratory fees to have a single presorted sample of macroinvertebrate
specimens identified down to the lowest practical taxonomic level, usually genus or species.
If you plan to have volunteers identify macroinvertebrates in the field, they’ll need ice cube trays for separating macroinvertebrates into families and macroinvertebrate identification informational resources. Two online identification resources are listed among the Informational Resources on the back page – a guide to macroinvertebrates of the Upper Midwest and a flow chart for identifying common macroin vertebrates found in Wisconsin wetlands.
Tubifex worm: Jason Neuswanger, troutnut.com; scud and black fly: Missouri Dept. of Conservation; mayfly: Dr. Stanley Szczytko, UWStevens Point
Monitoring Your Wetland – Benthic Macroinvertebrates
7
a primer to site-level monitoring activities for volunteer coordinatorsyourMonitoring
Wetland
. . . . . . . . . . . . . . . . . . . . . . . . . . .Macroinvertebrates INFORMATIONAL RESOURCES
Minnesota Pollution Control Agency Macroinvertebrate Community Sampling Protocol for Depressional Wetland Monitoring Sites This document outlines protocol developed for expertled volunteer monitoring of depressional wetlands in Minnesota. The methods are designed to use macroinvertebrate monitoring as a means of assessing water quality and require identification of macroinvertebrates down to the genus or species level using microscopes. (see website link on wetland monitoring site)
Field Testing the Wisconsin Depressional Wetland Macroinvertebrate and Plant Indices of Biological Integrity for Application by Trained VolunteersThis report documents the effectiveness of Wisconsin Department of Natural Resources developed macroinvertebrate and plant biotic index for assessment of isolated depressional wetlands in Wisconsin, when used by DNR biologists not trained in aquatic entomology. (see website link on wetland monitoring site)
Great Lakes Coastal Wetlands Consortium Great Lakes Coastal Wetlands Monitoring PlanThe fourth chapter of this plan outlines protocol for monitoring macroinvertebrates in Great Lakes coastal wetlands. Requires laboratory identification of macroinvertebrates down to species and genus level. http://www.glc.org/wetlands/final-report.html
Water Action Volunteers Biotic IndexThis Web page and linked documents are designed to help volunteers monitor macroinvertebrates in streams as part of the Water Action Volunteers program. These guidelines cannot be used to monitor macroinvertebrates in wetlands, but can be used when wetland volunteers monitoring a wetland in which there is a stream would like to monitor the stream.
http://watermonitoring.uwex.edu/wav/monitoring/biotic.html
University of Minnesota, Guide to the Aquatic Invertebrates of the Upper Midwest This guide provides an identification key for common aquatic invertebrates found in the Upper Midwest, based on mature larvae or adults.
http://wrc.umn.edu/pubs/watersqq/guidetoaquaticinverts
Wisconsin Department of Natural Resources and University of Wisconsin Extension Key to Life in the WetlandsThis is a flow chart for identifying common macroinvertebrates found in many Wisconsin wetlands. This chart is handy for identification of macroinvertebrates in the field.
http://watermonitoring.uwex.edu/pdf/level3/WEPP/WEPPLifeinWetland.pdf
University of Wisconsin-Superior and University of Wisconsin-Stevens Point Laboratory Identification ServicesFor macroinvertebrate identification contact either UWSuperior Aquatic Entomology Assistant Professor Kurt Schmude, [email protected] (715)3948421 or UWStevens Point Aquatic Entomology Laboratory Supervisor & Research Specialist Jeff Dimick, [email protected] (715)3463868
March 2011
Project coordination by the Rock River Coalition and Suzanne Wade, UWExtension Basin Education Initiative.
Researched and written by Patrice Kohl
With editorial contributions from Kurt Schmude University of WisconsinSuperior assistant professor, aquatic entomology; and Dick Lillie, biologist. Editorial assistance by Marie Martinelle and graphic design by Jeffrey J. Strobel, UWExtension Environmental Resources Center. Photography by Kris Stepenuck/Citizen Monitoring except where otherwise noted.
Project funded through a DNR CitizenBased Monitoring Partnership Program Grant with support from University of WisconsinExtension.
University of Wisconsin, U.S. Department of Agriculture and Wisconsin counties cooperating. An EEO/AA employer, University of Wisconsin Extension provides equal opportunities in employment and programming, including Title IX and American with Disabilities (ADA) requirements.
The Monitoring Your Wetland series includes 9 sections:
•IntroductiontoWetland Monitoring
•Birds
•SmallMammals
•Dragonflies&Damselflies (Odonata)
•FrogsandToads(Anurans)
•Butterflies(Lepidoptera)
•InvasivePlants
•WaterQuality
Macroinvertebrates
Available online in pdf format at:
wetlandmonitoring.uwex.edu
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .