molecular pathology – cell cycle dr. leonard da silva senior lecturer molecular & cellular...
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Molecular Pathology – Cell cycle
Dr. Leonard Da Silva
Senior Lecturer
Molecular & Cellular Pathology
Molecular Pathology
• study and diagnosis of disease molecules organs, tissues or fluids
• anatomic pathology, clinical pathology, molecular biology, biochemistry, proteomics and genetics
Tissue sources
Time Line
• 1902 Boveri – abnormal chromosomal segregation
• 1960’s Philadelphia Chromosome
• 1970’s – oncogenes – 2 hit hypothesis
• 1980’s cloning of RB
• 1990’s BRCA1/2
• 2000 Human genome
Grade, Stage & Prognosis
Cancer As A Disease Of Genetic Material
Heritable predisposition - Retinoblastoma
Chromosomal Abnormalities - Burkitt’s
Rare genetic disorders have inability to repair DNA e.g.
Xeroderma pigmentosa
Many chemical carcinogens are also mutagens
Transfer of DNA from tumour cells to normal cells leads
to transformation
Cancer Producing Genes Any mutated gene that contributes to neoplastic transformation
• Oncogenes
• Tumour suppressor genes
• Stability/DNA repair genes
Dominant Oncogenes
Identified as transforming genes in viruses
Altered forms of normal cellular genes - Proto-oncogenes
Products of oncogenes involved in:- Cell cycle- Cell division- Differentiation
This maintenance is lost in cancer cells
Control of normal cellular Growth & Differentiationmediated by:
Intracellular pathways activated
Activation / Repression of various genes
Growth Factors Growth Factor Receptors Cytokines
Dominant Oncogenes
Examples of Dominant Oncogenes
abl CML translocation
bcl2 Follicular Lymphoma translocation
erbB-2 Breast/ovarian carcinoma amplification
c-myc Burkitt’s lymphoma translocation
ras Thyroid /Colon carcinoma point mutation
ret Thyroid carcinoma Rearrangement
Tumour Suppressor Genes
Loss of function
Familial Syndromes
- Retinoblastoma
- Familial Adenomatous Polyposis
a b c d
a d
a c
b c
b d
Retinoblastoma
Examples of Tumour Suppressor Genes
APC Colon Cancer
BRCA1 Breast & Ovarian Cancer
BRCA2 Breast Cancer
NF1 Neurofibromatosis (malignant neurofibromas)
TP53 Brain, Breast, Colon, Liver, Lung carcinomas
RB Retinoblastoma, Sarcomas, Bladder
WT1 Wilm’s tumour
Dominant Oncogenes
Tumour Suppressor Genes
Enhanced Reduced
ActivatingGain in function
Dominant
InactivatingLoss of function
Recessive
DNA REPAIR
• Homologous recombination (HRR)
• Non-homologous end joining (NEHJ)
• Nucleotide excision (NER)
• Base excision (BER)
• Mismatch Repair (MMR)
Mechanism of Mutations
Point Mutations
Amplification
Translocation /
Rearrangements
Deletions
Altered Expression
Point Mutation
Change in single base-paire.g. G:C to A:T
SHE HAD ONE MAD CAT AND ONE SAD RAT
SHE HAD ONE BAD CAT AND ONE SAD RAT
8 14 8 14
C-myc
IgH
Translocation
Amplification
N-Myc Gene in NeuroblastomaCerbB2 gene in Breast Cancer
HER2 amplified HER2 non-amplified
Acquired from Vysis Educational Slide Set
Deletions
Tumour suppressor genes
e.g. retinoblastoma
Which cell does cancer arise in?
MULTISTEP MODEL OF CARCINOGENESIS
Principles of mammographic screening
Cell cycle
• The cell cycle is an ordered set of events
The cell cycle
Key regulators
• Cdk (cyclin dependent kinase, adds phosphate to a protein), along with cyclins, are major control switches
Master regulators
Checkpoints
P53 and cell cycle
• p53 is a protein that functions to block the cell cycle if the DNA is damaged. If the damage is severe this protein can cause apoptosis (cell death).
• p53 levels are increased in damaged cells. This allows time to repair DNA by blocking the cell cycle.
• A p53 mutation is the most frequent mutation leading to cancer Li Fraumeni syndrome, where a genetic a defect in p53 leads to a high frequency of cancer in affected individuals.
HPV pathogenesis
HPV pathogenesis
cell cycle <=> patient care