molecular biology_2
DESCRIPTION
Molecular BiologyTRANSCRIPT
-
???????????
?????????????????????????????????DNA ? RNA??????????????????????????envelope?????????????????????????????????????????????????????????????????????????????????????????????????????
?????????? ?????????????? RNA???????????? B??????????????
??????????
1.
2.
3.
4.
???????????????????????????????????????????????????? B??? DNA?? 3 kb???????????????? 4??????????????? 300 kb?????????????????????????????????????????????????????? B???????
???????? DNA??????? RNA??????????????????? DNA? RNA????????????????????????? DNA? RNA?????????????????????????????????????????????? DNA ?????????????????? DNA?????????????? RNA??????????????? RNA??????????? DNA????????? DNA??????? RNA?????????? RNA???
?? RNA ????????????????????????????? RNA?????????????????????? RNA??????????? RNA??????? RNA???????????????????????????????? RNA ??????? 10 ??? RNA??????? RNA????????????????????????DNA???????????
????????? DNA ???????????????????????????????????????? DNA??????????????????????????????????????????????? 1977? Sanger????X174??????X174????? DNA?
??????? ??? ?? 3
-
?????????????????????????????? 11??????????? 25??????? 6,078??????????????? DNA???? 5,375????????????? 20????????Sanger????X174? 11????????????????????????????????????????
?1?
?2?
?3?
????????????
5.
6.
???????????????????????????????????????????????????
????????????????????????
????????????????????????????????????????????? (?TAATG)????????????DNA ??????????? mRNA??????????(Reading frame)??????????????????????????? SV-40 DNA????????????? VP1?VP2?VP3 ????? 122 ???????????????????? t ?????? T ???????????????????
????X174?????? A,B,C??????????????
??????????????????????????????????????? DNA ???????????X174 ?????????217/5,375?G4 DNA?? 282/5,577???? 5?????? DNA?????????????????X174 ? H ??? A ????????3,906?3,973??? 67?????? RNA??????????????????????????????????????????????????????? 8.0 Kb?????????? 1.0 kb??????????????????
????? DNA ???????????????? rRNA ?????????????????????????????????????????????????? mRNA ????????? mRNA?polycistronie mRNA???????????????? mRNA?????????????? 12?????????????????????????????? mRNA????????? mRNA??????????????????????????X174????? D-E-J-F-G-H???????? mRNA??????????????????F?G? H???
??????? ??? ?? 4
-
???????D ???????????E ??????????????????????
7.
8.
1.
?????????????????????????????????????????????????????
??????????????????????????????????????(Intron)????? RNA ???????????????????? mRNA??????????????????? mRNA??????????????????????????????????????????????????(Exon)?? SV 40??????polyoma virus???????????SV 40???????T?? t???????? 5,146??????????? T????? 2,676?????? t??? 4,624?????????? 4,900? 4,555???? 346 bp????? T???????????????? 4,900?4,624??? DNA????? t??????????????????? T???????????? T? t????????
??????????????
??????papilloma virus????????????????????????? DNA????????????papova virus????????????????????????BPV?????????HPV????????????????????????????? BPV ??????????????????BPV DNA?? 7,94 5bp?????????????????????(Histone)????????? BPV DNA???? Hpa???????? G ????? 5'?3'???????????DNA ???????????????ORF??????? DNA???????????????BPV ?????????????NCR????????????????????????????E???????????L???
?????NCR?????????????URR???????LCR???????? L1?????????? E6???????????????????????????? BPV??? 1.0 kb?? NCR???????????????????????????????????????????? E2?????????????? AAC?????????? BPV NCR ??????????? TTGGCGGNNG ?ATCGGTGCACCGAT ??(Palindromic)???? NCR ?????????????????? BPV??????
??????? ??? ?? 5
-
2.
3.
????????????????E ??BPV ? E ??????????ORF???? E6?E7?E8?E1?E2?E3?E4?E5??? E6?E7?E1????????E8??? E1??E3?E4????? E2??E5? E2?????E2ORF ????????? NCR ??????????????????????????E2ORF? E1ORF???????????DNA????????????????????E6? E7ORFs??????????????E6? E7???????????????????? E6?E7 ??????????????????????????[1]? E6?E7???????????? Cys-x-x-Cys ?????????????????????????????????? E6?E7??? DNA?????????????????????????????????????????????[2]??????????????????????53 KD? 106 KD??? p53? p106????????????????????????????????????? E7? E6??????? p53? p106 ???????????????? E6 ? E7 ????????????????
??????????????L???L? ORFs????? L1? L2ORF???????????????? L1??????????L2??????????
RNA????????????
????????????RNA????MS2?R17?f2? Q???????????? 3,600? 4,200???????????MS2?R17? f2?????????????????????????????????????? A??????? 1,178????A?????????????????????????? RNA??????????????????????? A ????????????? 399 ???????????????????????? 180?????????????? RNA???????????????????????????????????????????????????? MS2?R17?f2 ????????????RNA ???????????????? RNase ?????????????????5'? 3'????????????????? RNA?????????
??????? ??? ?? 6
-
????????MS2?R17?f2? Q?????????? 5???
??? RNA ??? Q??????????? RNA ?????????????[1]????????????????? A2????????Maturaiton Protein????????????[2]?????????? A1?
B??????????????
B????HBV????? DNA????????????????????? 3.2 kb???? 2/3??????1/3????????DNA???????????? 5'?? 3'???????????????????????5'?? 250-300 ????????????????????????????????? 1.6-2.8 kb????? 2/3????????????????DNA?????B?????????????????? DNA??????????????????????????????????????HBV?????????????????????????
???????????HBV ????????????? 4 ????????????C?????S?????????????????????????????? X?? S???????? ORFs? S?? ORF??????????? ORFS????????? S?????????????????????????????????? -S1(pre-S1)???S2(pre-S2)????? ORFC ??????? ORF????C(pre-C)??????? C??????????? ORF???? DNA???????? S?????????????X????????C?????C?????????????Miller ??? HBV ????????? ORF?? ORF-5 ?ORF-6???? ORFs? X??????? ORF6????? DNA????????? DNA?????? ORF??????????
??????????????HBV???????????????HBV?????????????????????DR1? DR2?? U5?????
??????? ??? ?? 7
-
????????? U5????????DR1? U5????CORF????? DNA????????DR2??????? X??????? DNA??????????
?HBV???????????? 4??[1]???[2]????[3]polyA?????[4]??????????GRE???? HBV???????????? 3? HBV mRNA?????????????????????????5'?????? 3? RNA????????????????????????????????????????????ENH?????????polyA?????? CORF??? GRE?? SORF???????GRE????????? DNA?????????????????????
GRE??????????[1]?????????[2]?????????????[3]???????????????????
???????HBV?????????????????????????HBV DNA????????????????? DNA??????????? HBV DNA??????????????????? DNA????????????+?? RNA?????? RNA????+?? RNA??????????????????? DNA?????????????????????????? RNA??????????????? DNA???????????????? DNA ???????????? DNA ?5'???????????????? DNA???????? DNA????? RNA ??????????????????????????????????? DNA ??????????????????? DNA ???????
??????? ??? ?? 8