Laser Micromanipulation System

mmi CellManipulator

Molecular Machines & Industries

THE POWER OF INVISIBLE LIGHT: A NEW GENERATION OF MULTIBEAM OPTICAL TWEEZERSThe mmi CellManipulator features an intensely focused laser beam to hold, move, rotate, join, separate and stretch up to ten microscopic objects simultaneously.

A The mmi CellManipulator is an optical trap that enables precise, contact free mani -pulation of microscopic particles. It is capable of manipulating micrometer-sized dielec-tric particles, living cells, or subcellular organisms by exerting pico Newton forces via a highly focused laser beam. The beam is focused by sending it through a microscope objective. The narrowest point of the focused beam, known as the beam waist, contains a very strong light gradient. Dielectric particles are attracted along the gradient to the region of brightest light, the center of the beam. Using an infrared laser an invisible optical trap is created.

The mmi CellManipulator traps particles using a high-quality, Nd:Yag infrared laser with a wavelength of 1064nm and 3W maximum power.

Specially designed optics focuses the laser beam through an additional port without impairing any functionality of the inverted microscope. In addition, simultaneous use of phase contrast, DIC, fluorescence imaging, etc., together with trapping and force measurement is possible.

LEAD THE WAY TO MICROSCOPIC MANIPULATION

At MMI, we are committed to our customers and stand behind our products. We invite you to discover, innovate, and lead the way together with us.

Sincerely yours, the MMI Team

The Principle of Laser Micromanipulation

1 YAG Laser 1064 nm, 3.0 Watt

2 Focusing lens

3 Galvo scanners 2 kHz

4 Objective with high NA

5 Cells, particles in solution

LEAD THE WAY TO THE FUTURE

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ONE TOOL: MANY APPLICATIONSB Optical Tweezers are highly accurate instruments. In combination with the extreme sensitive quadrant detector technology, they are capable of the manipulation and detection of sub-nanometer displacements for sub-microm-eter di electric particles. Thus, they are often used to manipulate and study single molecules such as DNA, proteins, and enzyme interactions. The mmi CellManipulator is the only tool of its kind to offer customized and complete solutions in the following areas:

1. Molecular Motor Studies • Actin, Myosin interactions • Kinesin Motors • Dynein Motors

2. Measurements of Binding Forces • DNA studies • Viscosity measurements • Antibody, antigen binding forces • Bacterial adhesion studies • Virus to cell adhesion studies

3. Cell-based Studies • Cell fusions and cell-to-cell interactions • Implant studies • Intracellular manipulations • Study of neuronal networks • Drug effects on cells • Ca2+-channel studies

LEAD THE WAY TO A WIDE RANGE OF APPLICATIONS

Manipulation of tobacco plant cell

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Laser Trap

Pull with Optical Tweezers

Polystyrose sphere diffusely coated

with antigen

Silanized glass cover slip coated with antibodies

Plant Cell

LEAD THE WAY TO SCIENTIFIC RESULTS

mmi CELL MANIPULATOR APPLI CATIONS AT WORK: SELECTED REFERENCES

MOLECULAR MOTOR STUDIES:Josh Baker, Reno, Nevada, Biochemistry and Molecular Biology“Our current focus is on studying cooperative interactions among myosin motors in cells. Interestingly, myosin molecules function as force sensors as well as force gen-erators, thus when many myosin molecules function together in ensemble systems like muscle, the force generated by one myosin can cooperatively affect the biochemistry of neighboring myosin molecules. To better understand the interplay between myosin’s force generating and force sensing biochemistry, we use state-of-the-art experimen-tal techniques (laser traps, fluorescence microscopy, and single molecule imaging) to measure and systematically perturb (chemically, genetically, and biologically) the mechanics and chemistry of both single myosin motors and collective motor systems. We integrate these results into mathematical and computer models in an effort to better understand the molecular basis for cell motile processes ranging from muscle contrac-tion to intracellular transport.”

INTRACELLULAR INTERACTIONSUniversity of Wageningen, Laboratory of plant cell biology – The role of the cytoskel-eton in plant cell growth“Optical trapping allows manipulation of the cytoplasmic organization in live cells by trapping an organelle and dragging it away from the cytoplasm into the central vacuole. Between the position of the trapped organelle and the location of the cytoplasm from which it was dragged by the optical trap, a thread of cytoplasm is present. Our setup allows simultaneous optical trapping and confocal imaging.”

IMPLANT STUDIESUsing optical tweezers for measuring the interaction forces between human bone cells and implant surfaces: System design and force calibration, REVIEW OF SCIENTIFIC INSTRUMENTS 78, 2007

Martin Andersson, et al., Department of Chemistry, University of Florida, Gaines ville, Florida 32611

Stefan Niehren, Molecular Machines & Industries GmbH, Breslauerstrasse 2, 85386 Eching, Germany

“Optical tweezers were used to study the interaction and attachment of human bone cells to various types of medical implant materials…..In most other studies, the stiff-ness obtained by calibrating the trap with beads are used for measuring forces on more complicated structures such as cells. Here, the actual cells were used, which is a more straightforward method without the need for conversion.”

Ca2+ CHANNEL STUDIESStockholm, Karolinska, Diabetes Center: Am J Physiol Endocrinol Metab 288: E16-E28, 2005; doi:10.1152/ajpendo.00042.2004 0193-1849/05

ß-Cell Cav channel regulation in physiology and pathophysiology“Application of optical tweezers, fluorescence-activated cell sorting, and single-cell PCR will provide more detailed information on ß-cell CaV channel mRNA and protein isoforms.”

Manipulation of plant cell

Manipulation of chloroplast in plant cell

Overnight Experiment with living cells

TECHNOLOGYThe mmi CellManipulator’s versatility combines a 3W infrared laser, 2 fast gal va-no me tric mirrors, a high precision xy-stage, and a digital mmi CellCamera, with most scienti fic inverted mircroscopes.

C The advanced mmi CellManipulator maintains and controls up to 10 separate beams, each capable of manipulating one particle through the whole field of view. The beams are generated from one laser source by utilizing the high switching speeds of the galvanometric mirrors. In addition, any number of beams can be grouped for simul-taneous manipulation such as movement, rotation or stretching.

The optional installation of the quadrant detector based positioning measurement sys-tem enables precise nanometer scale position detection resulting in pico Newton scale force measurements. Moreover, the mmi CellManipulator system can be used on its own or in combination with other components, such as the mmi CellCut Plus microdis-section system, to create a unique microdissection and micromanipulation instrument for highly advanced research.

With multiple ports and dual-level laser integration, the microscope offers „optical bench flexibility“, enabling the seamless use of different modules and imaging technologies. The system-integrated digital camera is connected to the standard left side port; how-ever, other cameras may be added at different ports according to individual needs.

mmi CELLTOOLS SOFTWARED The optical tweezer system is controlled by the user-friendly mmi CellTools soft-ware, which provides an easy-to-use interface for simple creation, organization, and manipulation of traps. The software tools provide the advanced, function of moving up to 10 independent laser beams according to your unique research needs. Additional advantages are live video presentations, recordings with sample scanning, and docu-mentation through screenshots.

UPGRADES AND OPTIONSE The modular concept of the mmi instruments offers a highly flexible instrumentplatform, where optical tweezers, mecha nical micromanipulation, fluorescence and other modules can be integrated to meet exactly your requirements. The biggest benefit for our customers is an instrument which is easy to use without limiting the capability of performing the most complex research.

F 4 Quadrant Detector (QD) based positioning measurement system (MBPS) • Nanometer position measurements precision

• Dual head capacity to measure 2 objects simultaneously

• Microdissection: combine the mmi CellManipulator with the mmi CellCut Plus to expand research possibilities

• Fluorescence without limits: dual-level coupling of the solid state UV-cutting laser ensures full use of the whole range of fluorescence applications

• Piezo driven, software controlled Z-Focus

• Pen Screen: Can easily be integrated to operate the mmi CellManipulator system

LEAD THE WAY TO THE NEXT GENERATION TECHNOLOGY

Upgrades and OptionsE

mmi CellTools SoftwareD

TechnologyC

4 Quadrant DetectorF

The manufacturer reserves the right to make technical changes without prior notice. May 2008

CELLMANIPULATOR SPECIFICATIONS

Item SpecificationIR Laser, Nd:YAG, continuous wave Computer controlled

Power: 3W

Wavelength: 1064 nm

Beam quality: M2<1,5, TEM00

System complies with IEC 60825-1 Am. 2: 2001 Class 4 laser product

Invisible laser radiation, avoid eye or skin exposure to direct or scattered radiation

mmi CellCamera DXA285cF Digital colour: 1,392 x 1,040 pixels

DXA285F Digital monochrome: 1,392 x 1,040 pixels

Compact housing and FireWire connection

Galvanometer Ultra fast (2kHz) for beam steering

mmi CellTools software controlled

PC and Monitor Specifications will be continuously updated according to market developments

Windows XP Professional, 19” LCD Monitor (with high contrast)

mmi CellTools Software Laser energy and focus control

Full slide control

Live video display and video recording

Image saving for documentation and publication

Creation of traps by simple mouse click

Traps can be organized in groups

Rotation of trap groups

Stretching and contracting of trap groups

Click and drag feature for traps

xy stage control, down to 75nm step control (extremely smooth movement)

stage velocities adjustable between 0.005mm/sec, single step (75nm) movement mode at 10mm/sec

traps creation over full field of view

galvo control down to 50nm steps

Motorized stage Software controlled for high-precise movement

Travelling range: 120 x 100nm

Step width: 0,075μm

Repositioning accuracy: 1μm

Options:

Pen Screen system operation Sensitive 17” or 21” touch screen monitor for user friendly system operation

to allow direct target identification with a special pen

Fluorescence:

HBO standard 100W Fluorescence

cold light, no adjustments, 10x longer lifespan

X-Cite metal halide illuminator

TIRF attachment

DIC and Phase contrast

Quadrant detector based positioning measurement system precision to nanometer position measurements

(MBPS) dual head capacity to measure 2 objects simultaneously

integrated data acquisition with up to 50000 samples/sec

includes a PCI data acquisition board

mounted on a standard microscope camera port

Incubation box temperature and CO2 control

TTL interface synchronization with external systems

Piezo driven z-focus for objective software controlled

Anti-vibration table 120cm x 90cm with 25mm x 25mm M6 thread pattern (recommended option)

Possible mmi CellManipulator system upgrademmi CellCut Laser microdissection system The ultimate laser microdissection tool

to isolate groups of cells, single cells and cell components for analysis

MMI GmbH

Breslauer Strasse 2

85386 Eching, Germany

Phone : +49 89 319 048 40

Fax : +49 89 319 048 59

Mail : info@molecular-machines.com

www.molecular-machines.com

MMI AG

Flughofstrasse 37

8152 Glattbrugg, Switzerland

Phone: +41 44 809 10 10

Fax: +41 44 809 10 11

Mail: info@molecular-machines.com

www.molecular-machines.com

MMI Inc.

P.O. Box 348

Haslett, MI 48840, USA

Phone: +1 603 629 9536

Fax: +1 321 978 0304

Mail: sales_us@molecular-machines.com

www.molecular-machines.com