microfluidic perfusion system for yeast microscopy · microfluidic perfusion system for yeast...
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MICROFLUIDIC PERFUSION SYSTEM FOR YEAST MICROSCOPY
Bringing Microfluidics to Life www.cellasic.com | July 2010
Dynamic Solution Exposure
Contact: [email protected]
We developed a microfluidic plate for live cell imaging that maintains yeast in a single focal plane for long term, high magnification perfusion experiments. The plate contains 4 parallel flow units, each with 6 upstream solution inlets. The chambers accommodate cells from 4-10 micron in size.
Microfluidic Perfusion Plate
Yeast Live Cell Imaging
Philip Lee, Terry Gaige, and Paul HungCellASIC Corp., San Leandro, CA, USA
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Flow
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ocit
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ec)
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Rat
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l/hr
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Pressure (psi)
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nsit
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FITC
CY3
Maintains cells and daughters in single focal plane with perfusion
Clear optics with #1.5 thickness (170 um) glass coverslide bottom
Different height traps for yeast cell size variations
Key Features
Microfluidic Plate Yeast Chambers Flow Control System
Trapping site at rest Pressure driven loading of cells
Release pressure, physical trap of cells
trap height
elastic ceiling
glass floor
Before Loading 5 Seconds at 8 psi
250 um
Cell Trapping Mechanism
Budding Yeast
Fission Yeast
Flow Rates Switch Times
6 Flow Inlets
4 Fl
ow
Un
its
Imaging Window
Outlet Cell In
6 Fl
ow
Inle
ts
Outlets
4.0 um Trap
5.0 um Trap
4.5 um Trap
1 mm
Yeast Chamber
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10 hrs8 hrs6 hrs
4 hrs2 hrs
S. cerevisiae cultured in the microfluidic chamber over 10 hours. Perfusion rate of 4 ul/hr of SC complete medium at 30˚C. Cells imaged in the 4.0 um trap with a 40X objective.