micro notes gi_13_student
TRANSCRIPT
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Gastrointestinal Infections & Food Poisoning
MLAB 2434 –Microbiology Keri Brophy-Martinez
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General Concepts A complete history should be taken
Foods eaten recently Exposure to ill patients Recent travel Medicinal history Any underlying illnesses
Diarrhea may be caused by viruses, bacteria, parasites, food poisoning and non-infectious processes
Usually acquired by ingesting contaminated food or beverage
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Anatomy of GI Tract Organisms must be able to survive
gastric acids in order to reach the small bowel
In small bowel, motility (peristalsis) is major host defense. Organisms can not adhere to intestinal wall
Generally, a large dose of organisms is needed to cause disease
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Normal GI Flora Stomach contains few organisms Upper part of small bowel contains
small numbers of Enterococcus sp, lactobacilli, and diphtheroids, along with Candida albicans in 20-40% of individuals
Colon contains large numbers of anaerobes and facultative aerobes in 1000:1 ratio Colon produces IgA Pathogens would have to compete with
normal flora
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Risk Factors Number of Ingested Organisms
Median infectious dose (ID50)• The number of ingested organisms that
must be ingested to cause a diarrheal illness in 50% of exposed individuals
AchlorhydriaInadequate stomach acidity
Reduction in normal floraUse of antibiotics
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HistoryTravel to endemic areas of worldRecreational activitiesExposure to ill patients
FoodDetailed history of food eaten 3
days prior to onset of symptoms
Diagnosing Cause of Diarrhea
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Diagnosing Cause of Diarrhea Physical Exam
Dehydration Toxic megacolon Increase in heart rate or decrease in blood
pressure after standing upright
Laboratory Diagnosis CBC Fecal WBC Stool Culture O &P Electrolyte panel
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Enterotoxin-Mediated Diarrhea Symptoms
Rapid onset of diarrhea• Less than 12 hours
Lack feverAbsence of blood or pus
• Point to enterotoxin mediated illnessLarge number of watery stools
• Sometimes >20 per day
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Enterotoxin-Mediated Diarrhea Pathogens
ETECV. choleraeS. aureusC. perfringensB. cereus
Other typesViral and parasitic can be similar but
symptoms longer lasting
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Invasive Diarrhea Invasion of the bowel mucosal surface Symptoms
Fecal leukocytes RBCs Sometimes fever
Organisms Salmonella spp. Campylobacter spp. Shigella spp. E. coli E. histolitica
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Common Bacterial Agents Causing Diarrhea
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Common Bacterial Agents Causing Diarrhea Campylobacter jejuni
Most common cause of bacterial diarrhea in the world
Inadequately cooked poultry, untreated water, unpasteurized milk, and exposure to animals with diarrhea
Self-limiting, antibiotics not needed Manifests with fever, diarrhea and
abdominal cramping
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Campylobacter jejuni:Fast facts Grows best at 42 degrees C Microaerophilic conditions Capnophilic conditions Campy plate Gram-negative curved rods,
“seagull wings”
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Common Bacterial Agents Causing Diarrhea (cont’d) Salmonella species
Gastroenteritis and Food Poisoning• Contracted by eating undercooked
meat, poultry, eggs, and contaminated dairy products
• Nausea, vomiting, and diarrhea 6-48 hours after ingestion
• Usually self-limiting; antibiotics discouraged because they can induce “carrier state”
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Common Bacterial Agents Causing Diarrhea (cont’d) Salmonella species (cont’d)
Enteric Fever• Typhoid fever is most severe• Contaminated food and water• Organisms invade small bowel & colonic
tissue• Live and reproduce in monocytes• Can invade gall bladder and produce
“carrier state”• Symptoms include headache, fever, malaise
and abdominal tenderness
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Salmonella speciesFast Facts Requires a high microbial load for infection Culture
Look for LN on MAC Look for blue green colonies with blk
centers on HE Look for red colonies with blk centers on
XLD Fecal wbc lab test
See rbc’s and wbc’s in stool
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Salmonella
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Common Bacterial Agents Causing Diarrhea (cont’d) Shigella species
Diarrhea may have blood and pus Symptoms appear 12-50 hours after
exposure Most communicable of the diarrheal
bacteria Symptoms include: fever, malaise,
fatigue and anorexia
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Shigella speciesFast Facts Requires a low microbial load Fecal WBC lab test
Observe blood, WBCs, pus Culture
Colorless colonies(NLF) on MACBlue green colonies of HERed/colorless on XLD
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Shigella
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Common Bacterial Agents Causing Diarrhea (cont’d) Diarrheogenic Escherichia coli
ETEC: Enterotoxigenic• Cause of traveler’s diarrhea
EIEC: Enteroinvasive EPEC: Enteropathogenic
• Diarrhea outbreaks in infants in hospital setting EHEC: Enterohemorrhagic
• E. coli 0157:H7• Presence of shiga-like toxin• Associated with HUS
EAEC: Enteroaggregative• Chronic diarrhea in HIV patients, travelers, &
children in poor countries
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E. coli O157:H7 on MAC and SMAC
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Common Bacterial Agents Causing Diarrhea (cont’d) Vibrio species
Requires a large microbial load Utilize TCBS media
• Inhibits colonic flora• Differentiates sucrose fermenters from
species of Vibrio that are non-fermenters Stool contains no rbc’s or wbc’s since
it is toxin mediated & non-inflammatory
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Common Bacterial Agents Causing Diarrhea (cont’d) Clostridium difficle
Test for the toxin, culture not performed Hospital-acquired due to alteration of
normal flora and use of antibiotics Consists of Toxin A & B
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Less Common Agents of GI Illness Yersinia enterocolita Plesiomonas shigelloides Aeromonas hydrophilia Listeria monocytogenes
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Common Bacterial Agents Causing Diarrhea (cont’d) Many cases of food poisoning
caused by toxins produced by bacteriaBacteria may no longer be alive,
but toxins can cause food poisoning
Example: S. aureus, Clostridium botulinum, Bacillus cereus
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Bacterial Agents Associated with Food Poisoning
Agent Source of Contamination
Toxin Implicated Foods
S. aureus Nasal passages of asymptomatic carriers
Staphylococcal enterotoxin
Foods with mayonnaise, eggs, or dairy products, canned food, frozen food, processed meats
Clostridium botulinum
Soil & water Botulism neurotoxin
Mushrooms, salami, improperly canned foods
Bacillus cereus Environmental contaminant
Heat-stable enterotoxin & heat-labile enterotoxin
Grains, especially rice
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Common Parasitic Agents Causing Diarrhea Parasitic Infections
Giardia lamblia• Ingestion of contaminated water or person-person
spread• Nausea, vomiting, flatulence, cramping and diarrhea• Absence of fever and fecal leukocytes
Entamoeba histolytica• Fever, grossly bloody diarrhea
Other rare parasites include:• Cryptosporidium• Cyclospora• Microsporidia• Ascaris• Stronglyloides• Trichuris• And many more…
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Common Viral Agents Causing Diarrhea Hard to diagnosis due to virus
size Require secondary testing such
as cell culture, PCR, EM
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Common Viral Agents Causing Diarrhea Rotavirus
Primarily affects children < 5 years old
Spread by fecal-oral routePeak incidence from December
through June
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Common Viral Agents Causing Diarrhea Calicivirus
Norovirus• Spread by fecal-oral route by
contaminated food or water or environmental fomites, person to person
• Outbreaks on cruise ships• Cause of stomach flu
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Laboratory Diagnosis of Gastrointestinal Pathogens Specimen Collection and Handling
Collect with 4 days of onset of symptoms
Stool should be processed ASAP; NOT refrigerated
Rectal swabs NOT recommendedUse of preservatives not
recommended unless test ordered is an O & P
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Laboratory Diagnosis of Gastrointestinal Pathogens
Fecal Leukocytes• Direct microscopic exam almost
exclusively performed to detect presence of WBCs and RBCs. Their presence is due to intestinal wall bleeding
• Differentiates invasive disease from toxin-mediated illnesses, viral illness and parasitic infections
• + WBC: Salmonella, Shigella, Yersinia, EIEC, Campylobacter, Vibrio
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Positive Fecal WBC
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Interpretation of Cultures All media plates work together to
determine the presence of a pathogen. Routine stool cultures include testing
for:Salmonella species, Shigella species,
Aeromonas species, Plesiomonas species, and Campylobacter jejuni
Less frequently orderedYersinia, Vibrio, E. coli 0157:H7
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Identification & Reporting of Isolates Presumptive identification tests
Oxidase TSI Urea
Reporting If no pathogens found
• Report “No Salmonella, Shigella, or Campylobacter isolated”
Pathogen Isolated• Identification & quantification• Any amount significant• Additional testing may be indicated
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Treatment of Diarrhea Patients must be watched for dehydration Antibiotics are NOT effective against viral
pathogens, give supportive care for hydration Antibiotics may shorten illness by invasive
bacteria or an enterotoxin-mediated process Antidiarrheal mediations (Lomotil, Pepto-Bismol)
Primarily used with enterotoxin mediated diarrhea or viral gastroenteritis
Prophylactic therapy not recommended for travelers
When traveling, “Boil it, peel it, cook it, or forget it”!
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References Engelkirk, P., & Duben-Engelkirk, J. (2008). Laboratory
Diagnosis of Infectious Diseases: Essentials of Diagnostic Microbiology . Baltimore, MD: Lippincott Williams and Wilkins.
http://www2.cfpc.ca/cfp/2004/Nov/vol50-nov-cme-1.asp?stype=advanced&
http://www.cdc.gov/rotavirus/index.html Kiser, K. M., Payne, W. C., & Taff, T. A. (2011). Clinical
Laboratory Microbiology: A Practical Approach . Upper Saddle River, NJ: Pearson Education.
Mahon, C. R., Lehman, D. C., & Manuselis, G. (2011). Textbook of Diagnostic Microbiology (4th ed.). Maryland Heights, MO: Saunders.