MethodologyIsolation of Protein-Powdered non-fat milk was dissolved with distilled water then was heated at 40oC. Acetic acid was added to the solution until pH 4.6 was obtained. Filtration was done to obtain dry casein residue by air-drying. Alkaline Hydrolysis of Intact Protein-Extracted casein from skimmed milk was hydrolyzed with Sodium hydroxide and was autoclaved for 5 hours at 15 psi. After autoclaving, addition of distilled water was added then was neutralize by adding 6 M Hydrochloric acid then was tested with blue and red litmus paper.Qualitative Color Reactions-Alkaline Casein hydrolysate was tested with different reagents namely (Biuret Test, Ninhydrin Test, Xanthoproteic Test, Millons Test, Hopkins-Cole Test, Sakaguchi Test, Nitroprusside Test, Fohls Test, Test for Amides, and Pauly Test) and was observed with different color changes.In Biuret Test, Sodium hydroxide and Copper sulfate solution were added to the basic casein hydrolysate. In Ninhydrin Test, 0.1% ninhydrin solution was added to the diluted basic hydrolysate sample then was heated in a boiling water bath.In Xanthoproteic Test, concentrated Nitric acid and concentrated Sodium hydroxide were slowly added to the diluted basic hydrolysate sample.In Millons Test, Millons reagent was added to the diluted basic hydrolysate sample.In Hopkins-Cole Test, Hopkins-Cole reagent was added to the basic hydrolysate sample. Inclination and slowly addition of concentrated Sulfuric acid were done.In Sakaguchi Test, 10% of Sodium hydroxide and 0.02% of naphthol solution were added to the basic hydrolysate sample and were left for 3 minutes. 2% of Sodium hypobromite was then added to the sample.In Nitroprusside Test, 3 M Sodium hydroxide and 2% nitroprusside solution were added to the basic hydrolysate sample.In Fohls Test, 30% Sodium hydroxide and 5% Lead acetate were added to the basic hydrolysate sample. The sample was placed in a boiling water bath.In the Test for Amide, 20% Sodium hydroxide was added to the basic hydrolysate sample. The sample was placed in a boiling water bath. A moistened red litmus paper was placed over the mouth of the test tube.In Pauly Test, diazo reagent was prepared by mixing 1% sulfanilic acid and 5% Sodium nitrite. 10% Sodium carbonate and diazo reagent was added to the basic hydrolysate sample.Separation and Identification of Amino Acid by Paper Chromatography-Thin Layer Chromatography plate (12x15cm) was prepared by marking the bottom with 1.5 cm margin. Thirteen points were marked equidistantly placing the standards namely (Tryptophan standard, Arginine standard, Proline standard, Cysteine standard, Serine standard, Aspartic acid standard, Tyrosine standard, Histidine standard, Glycine standard, Alanine standard) and samples namely (Acid hydrolysate, Base hydrolysate and Enzyme hydrolysate) with capillary tubes. The plate was placed inside the pre-calibrated chamber and was left undisturbed. After the solvents ascended, the plate was removed and was air-dried. One percent of ninhydrin reagent was sprayed over the dried plate and was oven for 3 minutes.