Membrane Transport III

Chapter 11

Selectivity of a K+ channel

DVD Clip 45

DVD clip 48

Patch Clamp Recording

Patch Clamp Recording

Patch Clamp Measurements of single voltage gated Na+ channel

3 experiments on the same patch

cumulative of 144 experiments

Resting Chemical Synapse

Active Chemical Synapse

Neuromuscular Junction in a Frog

Three Conformations of Acetyl Choline receptor at the neuromuscular junction

DVD Clip 49

Ion Channels at Neuromuscular Junction

Motor Neuron Cell body in the Spinal Chord

Motor Neuron Cell body in the Spinal Chord

thousands of axon terminals are stained red by antibody that recognizes a protein in synaptic vesicles

Chapter 12

Intracellular Compartments and Protein Sorting

Major Intracellular compartments of an animal cell

Volumes of major intracellular compartments in a liver cell

Relative amount of membrane types in 2 cell types

Complex Cortical Network of Endoplasmic Reticulum

Cross section of liver cell

Topological relationships between compartments of a eukaryotic cell

Roadmap ofprotein trafficinside cell

Two ways in which a sorting signal can bebuilt into a protein

Some typical signal sequences

Nuclear Pore complexes perforate the nuclear envelope

Nuclear pore complexes

Nuclear side of the nuclear envelope

Face on view of nuclear complexes without the membrane

Side view of 2 nuclear pore complexes

Transport through nuclear pore complexes occurs through free diffusion and active

transport

Nuclear import signal direst proteins to the nucleus

Single amino acid mutation in signal will prevent import into the nucleus

Nuclear import receptors bind to nuclear porins and nuclear localization signal of cargo protein

Different nuclear localization signals bind different import receptors

Ran GTP provides energy for nuclear protein import

Ran-GTP controls cargo loading and unloading