mbv2010/bio2140 colloquium, february 19. rules multiple choice only one correct answer 30-60 seconds...
TRANSCRIPT
MBV2010/BIO2140
Colloquium, February 19
RULES
• Multiple choice• Only one correct answer• 30-60 seconds to answer• No textbook, computer, mobile phone, please
There will be a prize for the group with the most correct answers.
Example
Below is a series of equations. Which numberswould fit logically into the last equation ?(13 – 4) : 9 = 1 (27 – 9) : 9 = 2(87 – 15) : 9 = 8 (68 - ??) : 9 = ??
A (68 - 14) : 9 = 6
B (68 - 5) : 9 = 7
C (68 - 23) : 9 = 5
D (68 - 32) : 9 = 4
0
Example
Below is a series of equations. Which numberswould fit logically into the last equation ?(13 – 4) : 9 = 1 (27 – 9) : 9 = 2(87 – 15) : 9 = 8 (68 - ??) : 9 = ??
A (68 - 14) : 9 = 6
B (68 - 5) : 9 = 7
C (68 - 23) : 9 = 5
D (68 - 32) : 9 = 4
0
Dideoxynucleotides are used in
A DNA sequencing
B Cloning of DNA
C PCR
D Southern hybridization
1
Dideoxynucleotides are used in
A DNA sequencing
B Cloning of DNA
C PCR
D Southern hybridization
1
Ribosomal RNAs
A code for ribosomal proteins
B function in transcription of genes
C are absent from mitochondria
D function in protein synthesis
2
Ribosomal RNAs
A code for ribosomal proteins
B function in transcription of genes
C are absent from mitochondria
D function in protein synthesis
2
The smallest human chromosome has a size of
A 32 x 106 bp
B 41 x 106 bp
C 47 x 106 bp
D 58 x 106 bp
3
The smallest human chromosome has a size of
A 32 x 106 bp
B 41 x 106 bp
C 47 x 106 bp
D 58 x 106 bp
3
The polymerase chain reaction (PCR) is used for
A amplifying proteins
B copying plasmids
C copying RNA
D amplifying DNA
4
The polymerase chain reaction (PCR) is used for
A amplifying proteins
B copying plasmids
C copying RNA
D amplifying DNA
4
DNA ligases
A catalyze the formation of ß-N- glycosidic bonds
B play a role in transcription of DNA
C catalyze the formation of phosphodiester bonds
D catalyze the formation of hydrogen bonds
5
DNA ligases
A catalyze the formation of ß-N- glycosidic bonds
B play a role in transcription of DNA
C catalyze the formation of phosphodiester bonds
D catalyze the formation of hydrogen bonds
5
Van der Waal’s forces
A are attractive forces between atoms
B are 10 times weaker than covalent bonds
C are strongest at an atom distance of ≈ 0.2 nm
D play a role in stabilization of the DNA double helix
6
Van der Waal’s forces
A are attractive forces between atoms
B are 10 times weaker than covalent bonds
C are strongest at an atom distance of ≈ 0.2 nm
D play a role in stabilization of the DNA double helix
6
What is shown in this figure?
A a random RNA structure
B a microRNA structure
C a small nuclear RNA structure
D a tRNA structure
7
What is shown in this figure?
A a random RNA structure
B a microRNA structure
C a small nuclear RNA structure
D a tRNA structure
7
Protein structures can be determined by
A mass spectrometry
B 2D-polyacrylamide gel electrophoresis
C x-ray diffraction
D liquid chromatography
8
Protein structures can be determined by
A mass spectrometry
B 2D-polyacrylamide gel electrophoresis
C x-ray diffraction
D liquid chromatography
8
The amino acid sequence of proteins can bedetermined by
A mass spectrometry
B 2D-polyacrylamide gel electrophoresis
C x-ray diffraction
D liquid chromatography
9
The amino acid sequence of proteins can bedetermined by
A mass spectrometry
B 2D-polyacrylamide gel electrophoresis
C x-ray diffraction
D liquid chromatography
9
Centromeres
A are present in all chromosomes
B are normally in the center of chromosomes
C are nucleosome-free sequences
D normally contain repetitive DNA
10
Centromeres
A are present in all chromosomes
B are normally in the center of chromosomes
C are nucleosome-free sequences
D normally contain repetitive DNA
10
SWI/SNF is a
A histone modification complex
B nucleosome remodeling complex
C nucleosome assembly complex
D none of the above
11
SWI/SNF is a
A histone modification complex
B nucleosome remodeling complex
C nucleosome assembly complex
D none of the above
11
What is shown in this figure?
A histone modifications in telomeres
B histone modifications in protein-coding genes
C replacement of histone H3
D replacement of histone H2A
12
What is shown in this figure?12
A histone modifications in telomeres
B histone modifications in protein-coding genes
C replacement of histone H3
D replacement of histone H2A
The average size of genes in bacterial genomes is
13
A 1000 – 1500 bp
B 500 – 1000 bp
C 3000 – 4000 bp
D 1500 – 3000 bp
The average size of genes in bacterial genomes is
13
A 1000 – 1500 bp
B 500 – 1000 bp
C 3000 – 4000 bp
D 1500 – 3000 bp
Table 8.2 part 1 of 2 Genomes 3 (© Garland Science 2007)
Vibrio cholerae
Deinococcusradiodurans
(Conan the bacterium)
What is the role of the SeqA protein in E. coli ?
14
A initiate replication
B prevent replication
C break hydrogen bonds
D relieve topological stress in DNA
What is the role of the SeqA protein in E. coli ?
14
A initiate replication
B prevent replication
C break hydrogen bonds
D relieve topological stress in DNA
SeqA
SeqA binds to hemimethylated DNA
CH3
When in a cell cycle is the eukaryotic origin recognition complex bound to DNA?
15
A in G1, S, and G2
B in S, G2, and M
C in G2, M, and G1
D only at the end of G1
When in a cell cycle is the eukaryotic origin recognition complex bound to DNA?
15
A in G1, S, and G2
B in S, G2, and M
C in G2, M, and G1
D only at the end of G1
Which of the following proteins is involvedin initiation of replication in the nucleus?
16
A DnaA
B Type II topoisomerases
C Cdc6
D Cdc45
Which of the following proteins is involvedin initiation of replication in the nucleus?
16
A DnaA
B Type II topoisomerases
C Cdc6
D Cdc45
Replication protein A (RPA) is a17
A helicase
B topoisomerase
C single-strand binding protein
D DNA polymerase
Replication protein A (RPA) is a17
A helicase
B topoisomerase
C single-strand binding protein
D DNA polymerase
The proliferating cell nuclear antigen (PCNA)is involved in
18
A cell division
B nucleosome assembly
C genome replication
D formation of
heterochromatin
The proliferating cell nuclear antigen (PCNA)is involved in
18
A cell division
B nucleosome assembly
C genome replication
D formation of
heterochromatin