maternal dietary n-3 fatty acids affect yolk and liver composition in broiler progeny
DESCRIPTION
Maternal dietary n-3 fatty acids affect yolk and liver composition in broiler progeny. Astrid Koppenol. Introduction. Yolk provides FA to embryo (80% absorbed ) YSM: uptake and transfer of lipids from yolk to embryonic circulation Consumption eggs : dietary yolk n-3 PUFA - PowerPoint PPT PresentationTRANSCRIPT
Maternal dietary n-3 fatty acids affect yolk and liver composition in broiler progeny
Astrid Koppenol
Introduction Yolk provides FA to embryo (80%
absorbed) YSM: uptake and transfer of lipids from yolk to embryonic circulation
Consumption eggs: dietary yolk n-3 PUFA
Broilers? n-3 PUFA eicosanoids immunity
- 50% of total amount of FA originally present in the yolk incorporated into tissues of chick- 50% of total FA present in yolk undergoes β-oxidation to provide energy
Objectives Investigate the effect of dietary FA’s in breeder feed
Arachidonic acid AA (20:4 n-6) Eicosapentaenoic acid EPA (20:5 n-3) Docosahexaenoic acid DHA (22:6 n-3)
on: FA composition of
Yolk Residual yolk Progeny liver
Experimental Design
4 T: 1. control (AA) 2. EPA=DHA 3. DHA>EPA 4. EPA> DHA
d1 d14 d30
Residual yolkFresh yolk Liver
RESULTS AND DISCUSSION
CON EPA=DHA DHA EPA
Wei
ght (
g)
0
10
20
30
40
50
60
EW YW RYW
a b ab ab
a b b b
a a b b
Significant differences in- Egg weight- Yolk weight- Residual yolk weightbetween control group and n-3 enriched groups
Egg, yolk and residual yolk weight
n-6 n-3 n-6/n-3
FA (%
)0
5
10
15
20
25
CON EPA=DHA DHA EPA
b b b
a
b
a a a
a
b b b
n-6 n-3 n-6/n-3
FA (%
)
0
5
10
15
20
25
30
CON EPA=DHA DHA EPA
b b b
a
b
a a a
a
b b b
RESULTS AND DISCUSSIONn-6/n-3 ratio
Control eggs:- more n-6 FA- Less n-3 FA
Dietary n-3 enrichment lowers n-6/n-3
Fresh yolk Residual yolk
RESULTS AND DISCUSSIONAA, EPA and DHA conc
Control group:- Higher AA conc- Lower DHA and EPA concDietary EPA - increases EPA and DHA in yolkDietary DHA - results in only higher DHA conc- Effect is less pronouncedAll enriched groups:- Much more DHA available for theProgeny compared to control groupAA EPA DHA
% F
A c
onc
0
1
2
3
4
CON EPA=DHA DHA EPA
a
ab b b
c c
b a
b
a ab
a FRESH YOLK
AA EPA DHA
% F
A c
onc
0,0
0,5
1,0
1,5
2,0CON EPA=DHA DHA EPA
b
a
ab
b b
a a
b bb
a a
a
Fresh yolk Residual yolk
RESULTS AND DISCUSSIONAA, EPA and DHA conc
LIVER D1
ab
AA EPA DHA
% F
A c
onc
0
2
4
6
CON EPA=DHA DHA EPA
b
a a a
b
a a
a
EPA conc in DHA group is increasedcompared to RY conc
All conc are higher
Biomagnification of essential PUFA
RESULTS AND DISCUSSIONBiomagnification of essential PUFA
= proportion of essential C20-22 FA increases each stage of transfer process from RY to embryo
1. Preferential uptake from yolk by YSM
2. In YSM: DHA translocated to TAG, resulting in enriched VLDL released into circulation
3. Preferential mobilisation from adipose TAG into plasma
Yolk RY L d1 L d14 L d30
conc
entra
tion
DH
A (%
)
0
1
2
3
4
5
6
7
CON EPA=DHA DHA EPA
b
aab
a
b
a a a
b
a a
a
b ab
a
ab
RESULTS AND DISCUSSION
Yolk RY L d1 L d14 L d30
conc
entra
tion
DH
A (%
)
0
1
2
3
4
5
6
7
CON EPA=DHA DHA EPA
b
aab
a
b
a a a
b
a a
a
b ab
a
ab
Yolk RY L d1 L d14 L d30co
ncen
tratio
n E
PA
(%)
0,0
0,2
0,4
0,6
0,8
1,0
CON EPA=DHA DHA EPA
c
b
c
a
b
a
b
a
b
aa
a
abab
a
b
DHA more present early in the post hatch life, while EPA remains low and equal inprogeny liver
DHA vs EPA
Dietary n-3 enrichment Decrease egg weight, yolk weight and residual yolk weight Lowers n-6/n-3 ratio in fresh yolk and residual yolk More DHA available for transfer to offspring from residual yolk
Adding DHA to maternal diet results in higher DHA concentration in fresh and residual yolk
Adding EPA to maternal diet results in more EPA and DHA in residual yolk
Biomagnification during transfer from RY to tissue of offspring
DHA present in higher conc early in post hatch life
CONCLUSION
Thank you for your attention!Any Questions?
Astrid Koppenol