manual hplc primaide hitachi

INSTRUCTION MANUAL

FOR

PRIMAIDE SYSTEM MANAGER (DETAILED OPERATION)

This system is intended for research use only.

It is not to be used for reporting patient diagnostic or therapeutic results.

Please read through this manual carefully

and keep it in a safe place.

Before using the instrument, read the safety

instructions and precautions carefully.

Keep this manual in a safe place nearby so it can

be referred to whenever needed.

Hitachi High-Technologies Corporation

24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, Japan

Copyright C Hitachi High-Technologies Corporation 2012. 1st Edition, 2012

All rights reserved. Printed in China. Part No. 895-9635

Be sure to read through and understand the following points

with regard to this manual.

1. Information contained in this document is subject to

change without notice for improvement.

2. This manual is copyrighted by Hitachi High-Technologies

Corporation with all rights reserved.

No part of this manual may be reproduced, transmitted

or disclosed to a third party in any form or by any means

without the express written permission of Hitachi

High-Technologies Corporation.

3. Hitachi High-Technologies Corporation assumes no

liability for any direct, indirect, or consequential damages

arising from use not described in this manual.

Utmost care must be exercised when using the

instrument.

4. This document does not provide any warranty or

permission for industrial properties or any rights to grant

license lawfully and without infringement.

PREFACE

Thank you very much for purchasing the Primaide System Manager.

The Primaide System Manager is the Chromato data station for high

performance liquid chromatography (HPLC).

This software is intended for use by persons having a basic

knowledge of chemical analysis.

Remember that improper use of analytical instruments,

chemicals or samples can result not only in wrong analytical

data but also in consequences adverse to safety. Note that only

persons with a basic knowledge of chemical analysis procedures

may use this instrument.

Carefully read this instruction manual before attempting operation. For

proper use of the software, please acquaint yourself with it.

ABOUT THIS MANUAL

The manual of the Primaide System Manager is consists of ;

(1) INSTRUCTION MANUAL FOR Primaide System Manager

(INSTALLATION)


(ADMINISTRATION)


(DETAILE OPERATION)


(MAINTENANCE SOFTWARE)

Manuals (2), (3) and (4) have been saved to the Instruction Manual

CD as PDF files.

These instruction manuals describe how to install and use the

Primaide System Manager. The operating procedures and points

that should be especially noted in regard to the software are

contained in this manual.

First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the

beginning of this manual to ensure safe operation of the

Primaide System Manager.

For operation of the Primaide System Manager read the each

instruction manual.

IMPORTANT - 1

IMPORTANT

Warranty on Product

The Primaide System Manager is warranted to be free from

defects in material or workmanship under normal use within the

product specifications indicated in this manual and under

conditions given below. This warranty is void if the software is

not used according to the instruction manual.

The manufacturer makes no warranties, either express or implied,

except as provided herein, including without limitation thereof,

warranties as to marketability or merchantability, for a particular

purpose or use, or against infringement of any patent.

No oral or written information or advice given by the

manufacturer, its dealers, distributors, agents or employees shall

create a warranty or in any way increase the scope of this

warranty.

(1) Scope of Warranty

Any parts that prove to be defective in design or

workmanship during the warranty period will be repaired,

adjusted or replaced without charge. A substitute part

may be used for repair, or replacement with an equivalent

product may be made instead of repair. Such system

components as a personal computer and printer to be

updated frequently for improvement may not be available in

original versions at the time of replacement.

Note that this warranty does not apply to the instrument

after it is discarded, or if modified by the user or resold

without permission from the manufacturer, consumable

parts, and any failure of lifetime-expired parts.

The manufacturer assumes no liability for any damage to

data or application software due to any possible fault or

failure of this instrument.

(2) Warranty Period

One year from the date of initial installation.

(In case a separate warranty document has been issued,

the warranty period indicated in it takes precedence over

the above period.)

IMPORTANT - 2

(3) Limitations and Exclusions on Warranty

Note that the following cases are excluded from the scope

of this warranty, i.e., these cases are beyond the coverage

of free-of-charge repair even during the warranty period

indicated above.

(a) Failure due to operation at a place not meeting the

installation requirements specified by the

manufacturer.

(b) Failure due to power supply voltage/frequency other

than specified by the manufacturer or due to

abnormality in power supply.

(c) Corrosion or deterioration of the piping due to

impurities contained in gas, compressed air or

cooling water supplied by the user.

(d) Corrosion of the electric circuits or deterioration of

the optical elements due to highly corrosive

atmospheric gas.

(e) Failure due to use of software, hardware or spare

parts not supplied by the manufacturer.

(f) Failure due to use not described in the manual or

improper repair not approved by the manufacturer.

(g) Failure due to maintenance or repair by other than

service personnel qualified by the manufacturer.

(h) Failure due to relocation or transport conducted not

under the supervision of the manufacturer after the

initial installation of the instrument.

(i) Failure due to disassembly, modification or relocation

not approved by the manufacturer.

(j) Failure due to acts of God, including fire, earthquake,

storm, flood, lightning, social disturbance, riot, crime,

insurrection, terrorism, war (declared or undeclared),

radioactive pollution, contamination with harmful

substances, etc.

IMPORTANT - 3

(k) Failure of the hardware, or damage to the system

software, application software or data due to

computer virus infection.

(l) After disposal of this instrument, after its resale

without prior approval from the manufacturer,

consumable parts, and failure of any part that have

reached the end of its service life.

(4) Disclaimer of Warranty

THE MANUFACTURER MAKES NO WARRANTIES,

EITHER EXPRESS OR IMPLIED, EXCEPT AS

PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION

THEREOF, WARRANTIES AS TO MARKETABILITY,

MERCHANTABILITY, FOR A PARTICULAR PURPOSE

OR USE, OR AGAINST INFRINGEMENT OF ANY

PATENT. IN NO EVENT SHALL THE MANUFACTURER

BE LIABLE FOR ANY DIRECT, INCIDENTAL OR

CONSEQUENTIAL DAMAGES OF ANY NATURE, OR

LOSSES OR EXPENSES RESULTING FROM ANY

DEFECTIVE PRODUCT OR THE USE OF ANY

PRODUCT.

NO ORAL OR WRITTEN INFORMATION OR ADVICE

GIVEN BY THE MANUFACTURER, ITS DEALERS,

DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL

CREATE A WARRANTY OR IN ANY WAY INCREASE

THE SCOPE OF THIS WARRANTY.

IMPORTANT - 4

Installation, Relocation and After-sale Technical Service

(1) Installation and Relocation

(a) Installation at delivery shall not be carried out by the

user. It shall be carried out by our sales

representative or the engineers who have been

trained and qualified for this purpose by us in order to

use the instrument safely and accurately.

(b) Before installation, the user shall make preparations

for satisfying the installation requirements in

accordance with this instruction manual.

(c) If relocation becomes necessary after initial

installation (delivery), please contact the dealer from

whom you purchased the instrument or our sales

representative.

(2) After-sales Service

(a) For after-sales service, contact our sales

representative or service office of our sales

representative.

(b) For service after the warranty period, consult us with

regard to a maintenance and inspection service

contract.

Technical Seminars and Training Courses for Users

We offer technical seminars and training courses at either our or

users facilities to ensure proper and safe operation of the

analytical instrument to its full performance. For further

information, contact our sales representative. (Applicants will

be charged.)

IMPORTANT - 5

Other Precautions

(1) Handling of Chemicals and Samples

(a) The user is responsible for following relevant legal

standards and regulations in handling, storage and

disposal of chemicals and samples used in analytical

operations with this instrument.

(b) Reagents, standard solutions and accuracy-control

samples shall be handled, stored and discarded as

instructed by the respective suppliers.

(2) Trademark Acknowledgements

Primaide System Manager is registered trademarks of

Hitachi High-Technologies Corporation.

Microsoft, MS, Microsoft Excel, Microsoft Word, and

Windows are either registered trademarks or trademarks of

Microsoft Corporation in the United States and other

countries.

Windows 7 is a trademark of Microsoft Corporation (USA).

All other trademarks are the property of their respective

holders and are hereby acknowledged.

SAFETY - 1

SAFETY SUMMARY

General Safety Guidelines

Before using the Primaide System Manager, be sure to read the

following safety instructions carefully.

The hazard warnings which appear on the warning labels on the

product or in the manual have one of the following alert headings

consisting of a safety alert symbol and signal word

DANGER, WARNING or CAUTION.

: Safety alert symbol used for calling

attention to a potential hazard which

could cause personal injury.

To avoid possible injury or death,

observe all the safety messages

following this symbol.

DANGER : Indicates an imminently hazardous

situation which, if not avoided, will result

in death or serious injury.

WARNING : Indicates a potentially hazardous

situation which, if not avoided, can

result in death or serious injury.

CAUTION : Indicates a hazardous situation which, if

not avoided, can result in minor or

moderate injury.

NOTICE : Indicates a hazardous situation which, if

avoided, can result in damage to

property.

In addition to the above, the following signal word is used to

indicate instructions for ensuring proper use of the product.

NOTE: Indicates an instruction for ensuring correct use

of the product and accurate analysis therewith.

SAFETY - 2

SAFETY SUMMARY

Common Safety Precautions

Prior to Use

Before using the product, be sure to read this instruction

manual carefully to attain a full understanding of its

operations.

Keep the instruction manual handy nearby so it can be

referred to whenever needed.

Be sure to observe the procedures specified in the manual.

Be sure to understand and follow all the safety instructions

given in the manual.

Be sure to observe all the hazard warnings attached to the

instrument or provided in the manual. Failure to do so could

result in personal injury or damage to the instrument.

Be sure to follow all the methods of use instructed in the

manual for proper application of the product.

Absolutely avoid modifying the product, using non-specified

parts, or removing safety devices as it could be hazardous.

Do not perform any operation or action other than described

in the manual.

On occurrence of any trouble in the instrument, notify the

nearest sales representative or service office of Hitachi High-

Technologies Corporation.

When using chemicals for the instrument, be sure to provide

proper ventilation of the room. Inadequate ventilation could

endanger human health.

Keep in mind that the hazard warnings in the manuals or on

the product cannot cover every possible case, as it is

impossible to predict and evaluate all circumstances

beforehand. Always be alert and use your common sense.

SAFETY - 3

SAFETY SUMMARY

Common Safety Precautions (Continued)

In Use

If an abnormality such as unusual noise, odor, fuming or gas

leakage occurs during operation of the instrument, immediately

disconnect power to the instrument, and take proper safety

measures as required. Then, notify the nearest Hitachi High-

Technologies Corporation sales representative or service office

of Hitachi High-Technologies Corporation sales representative.

Installation, Maintenance, and Relocation

At the time of delivery, installation of the instrument shall be

carried out by or under supervision of qualified service

personnel of the manufacturer or its authorized service agent

for ensuring safety and high accuracy in operation of the

instrument. It is not permitted for the user to carry out

installation.

After completion of installation, check that all the standard

parts are equipped. If the instrument is made active with any

one of the standard parts not equipped, a failure could occur

to result in a hazardous condition.

If any item is missing or damaged or if you have any question,

notify the installation personnel at site or the nearest Hitachi

High-Technologies Corporation sales representative or

service office of Hitachi High-Technologies Corporation sales

representative.

The maintenance and checkup procedures to be taken by the

user are only those described in the manual. When taking

the maintenance and checkup procedures described in the

manual, attain a clear understanding of them.

Do not perform other maintenance and checkup procedures

to avoid jeopardizing safety and causing troubles in the

instrument.

SAFETY - 4

SAFETY SUMMARY

Common Safety Precautions (Continued)

After installation, do not relocate the instrument. If the

instrument is relocated, vibration or impact to be applied

during relocation could cause a malfunction in the optical

components that have been adjusted precisely.

SAFETY - 5

SAFETY SUMMARY

Safety Instructions in This Manual

Shown below are the safety instructions contained in this manual

and their relevant sections in it.

DANGER Indications

The indication DANGER does not apply to this product.

WARNING Indications

The indication WARNING does not apply to this product.

CAUTION Indications

Fatigue due to Long-Hour Operation

If you keep working with the display monitor and keyboard for

long hours, your eyes and body will be fatigued to jeopardize

your health. When working with the display monitor for a long

time, take a break for 10 to 15 minutes per hour for health of

your eyes and body.

(Chapter 1)

SAFETY - 6

SAFETY SUMMARY

NOTICE

Accuracy and Precision of Measured Values

Carry out control sample measurements to ensure that the

performance of the instrument is normal.

Data Backup

Data on the hard disk may become unusable due to a system

failure, wrong operation, computer virus infection, etc.

To ensure data integrity in case of accidental damage to the hard

disk, periodically make backup copies of hard disk files onto

floppy disks. Be sure to carry out this data backup procedure

periodically.

Power Interruption

On occurrence of momentary power voltage drop due to power

interruption or lightning, the personal computer may become

faulty or the basic software, application software or data may be

damaged.

For protection against momentary power voltage drop, it is

advisable to use an AC uninterruptible power supply unit (stated

according to the Japanese Electronic Industry Development

Association guidelines for protection against momentary power

voltage drop in personal computers).

SAFETY - 7

SAFETY SUMMARY

NOTICE (Continued)

About Personal Computer

About handling of the personal computer, read the manual

that is standard-furnished for personal computer and use as

directed.

When using this product, be sure to observe the warnings

and cautions shown by the personal computer manufacturer.

Do not turn off the personal computer main circuit while the

hard disk or removable disk drive is active. If power to the

personal computer is turned off while the hard disk or

removable disk is being accessed, the personal computer

may become faulty or data/software stored in it may be

damaged. Before turning off power to the personal

computer, quit the Primaide System Manager and then take

the shutdown sequence of the operating system of the

personal computer.

Do not locate the personal computer on an unstable place or

a narrow place. Failure to do so could result in personal

injury or damage to the personal computer.

Do not spill the solvent on the personal computer because

there is a possibility that the personal computer fails. Should

you spill the solvent on the personal computer, wipe it off

immediately.

If you use as it is, it cause an electric shock, release of fume

and ignition.

Do not use the volatile liquid near the personal computer

because of the possibility of ignition hazard.

SAFETY - 8

SAFETY SUMMARY

NOTICE (Continued)

Protection against Computer Viruses

If any program/data is damaged suddenly or an unexpected

operation/screen is encountered, the personal computer may be

infected by a computer virus. Computer viruses are malicious

programs that sneak into personal computers to cause

misbehavior or damage to data. And, a program designed to

offer protection against computer viruses is called a vaccine

program.

Possible causes of virus infection are:

(a) Downloading a virus-laden program through

communication.

(b) Using a floppy disk or other storage medium infected by a

virus.

Note also that once a virus infects any personal computer, it

may spread to other computers via communication or storage

medium.

Never use a program or storage medium that is suspected of

containing a virus.

If there is a possibility of virus infection, check for a virus using a

vaccine program. Note, however, that some kinds of vaccine

programs cannot eradicate particular viruses.

In such a case, be sure to make a backup of hard disk files.

The user is requested to prepare a vaccine program and carry

out virus removal on his or her responsibility.

- i -

CONTENTS

PREFACE

ABOUT THIS MANUAL

IMPORTANT ....................................................................................................... IMPORTANT-1

Warranty on Product ................................................ IMPORTANT-1

Installation, Relocation and After-sale

Technical Service ..................................................... IMPORTANT-4

Technical Seminars and Training Courses for

Users ....................................................................... IMPORTANT-4

Other Precautions .................................................... IMPORTANT-5

SAFETY SUMMARY .................................................................................................. SAFETY-1

General Safety Guidelines ....................................... SAFETY-1

Common Safety Precautions ................................... SAFETY-2

Safety Instructions in This Manual ........................... SAFETY-5

DANGER Indications ..................................... SAFETY-5

WARNING Indications................................... SAFETY-5

CAUTION Indications .................................... SAFETY-5

Fatigue due to Long-Hour Operation ............ SAFETY-5

NOTICE .......................................................................... SAFETY-6

1. OVERVIEW ..................................................................................................................... 1-1

1.1 The Primaide System Manager ...................................... 1-1

1.2 The Main Function .......................................................... 1-2

2. STARTING/EXITING THE PRIMAIDE SYSTEM MANAGER ........................................... 2-1

2.1 Introduction .................................................................... 2-1

2.2 Starting/Exiting the Primaide System Manager ........... 2-2

2.3 Menu ................................................................................ 2-3

2.3.1 File Menu ............................................................. 2-3

2.3.2 Edit Menu ............................................................. 2-7

2.3.3 Window Menu ...................................................... 2-8

2.3.4 Help Menu ............................................................ 2-8

3. PRIMAIDE SYSTEM MANAGER MAIN WINDOW .......................................................... 3-1

3.1 Menu Command Function of Main Window ................. 3-3

3.1.1 File Menu ............................................................. 3-3

3.1.2 Help Menu ............................................................ 3-4

3.2 Main Tool Bar Icons ....................................................... 3-5

3.2.1 Change Application Icon ...................................... 3-6

3.2.2 Method Setup Icon ............................................... 3-6

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3.2.3 Sampler Setup Icon .............................................. 3-8

3.2.4 Acquire Data Icon ................................................. 3-8

3.2.5 Reprocess Data Icon ............................................ 3-9

3.2.6 Report Icon ........................................................ 3-10

3.2.7 System Status Icon ............................................ 3-11

3.2.8 Pump(A) ON/OFF and Pump(B) On/Off Icon ..... 3-13

3.2.9 Module Detailed Information Icon ....................... 3-13

3.2.10 Quick Analysis Start Icon ................................... 3-13

4. FUNCTION AND OPERATION OF METHOD WINDOW ................................................. 4-1

4.1 Menu Command Function of Main Window ................. 4-1

4.1.1 File Menu ............................................................. 4-2

4.1.2 Edit Menu ............................................................. 4-2

4.1.3 Module Setup Menu ............................................. 4-3

4.1.4 Data Process Setup Menu ................................... 4-4

4.1.5 Option Menu......................................................... 4-5

4.1.6 Window Menu ...................................................... 4-7

4.1.7 Help Menu ............................................................ 4-8

4.2 Using a Method ............................................................... 4-9

4.2.1 Opening an Existing Method .............................. 4-10

4.2.2 Creating a New Method ...................................... 4-11

4.2.3 Adding or Modifying Method Parameters ........... 4-12

4.2.4 Using Reference Methods .................................. 4-12

4.2.5 Saving a Method ................................................ 4-14

4.3 Module Setup Menu ...................................................... 4-15

4.3.1 Method Information ............................................ 4-15

4.3.2 Method Configuration ......................................... 4-16

4.3.3 Pump Setup ....................................................... 4-18

4.3.4 Autosampler Setup ............................................. 4-20

4.3.5 Column Oven Setup ........................................... 4-22

4.3.6 Channel 1 or 2 Detector Setup ........................... 4-23

4.3.7 1410 UV Detector Setup .................................... 4-23

4.3.8 USB-AID Setup (AID: Analog Input Device) ....... 4-26

4.3.9 1430 DAD Setup ................................................ 4-28

4.3.10 Time Summary ................................................... 4-30

4.4 Data Processing Setup Menu ...................................... 4-31

4.4.1 Channel 1 or Channel 2 ..................................... 4-31

4.4.2 Calculation Method ............................................. 4-31

4.4.3 Component Table ............................................... 4-36

4.4.4 Concentration Table ........................................... 4-40

4.4.5 Coefficient Table ................................................ 4-42

4.4.6 Integration Time Table ....................................... 4-44

4.4.7 DAD Data Processing ........................................ 4-47

4.4.8 Chromatogram Display Format .......................... 4-49

4.4.9 DAD Data Display .............................................. 4-52

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4.4.10 Confidence Report ............................................. 4-54

4.4.11 Report Format .................................................... 4-56

5. SAMPLE TABLE ............................................................................................................. 5-1

5.1 Sample Table setup ........................................................ 5-1

5.1.1 File Menu ............................................................. 5-2

5.1.2 Edit Menu ............................................................. 5-3

5.1.3 SampleSetup Menu and Sample Table Tool Bar .. 5-4

5.1.4 Window Menu ...................................................... 5-4

5.1.5 Help Menu ............................................................ 5-4

5.2 Using Sample Table ....................................................... 5-5

5.2.1 Opening an Existing Sample Table ...................... 5-5

5.2.2 Creating a Single-Method Sample Table .............. 5-5

5.2.3 Creating a Multi-Method Sample Table ................ 5-7

5.2.4 Editing a Sample Table During Data Acquisition .. 5-8

5.2.5 Saving a Sample Table ........................................ 5-9

5.3 Sample Table Window .................................................. 5-10

5.3.1 Setup Information Screen................................... 5-10

5.3.2 Edit Table Screen ............................................... 5-14

6. ACQUIRING DATA .......................................................................................................... 6-1

6.1 Data Acquisition Window............................................... 6-2

6.1.1 File Menu ............................................................. 6-3

6.1.2 Acquire Menu ....................................................... 6-4

6.1.3 Data Display Menu ............................................... 6-9

6.2 Running Data Acquisition ............................................ 6-12

6.2.1 Initiating the Idle Monitor .................................... 6-13

6.2.2 Starting a Run .................................................... 6-15

6.2.3 Starting Series ................................................... 6-16

6.2.4 Performing a Manual and Auto Noise Test ......... 6-20

6.2.5 Editing a Sample Table During Data Acquisition 6-22

6.2.6 Data Acquisition from arbitrary line of

Sample Table ..................................................... 6-23

6.2.7 Data Reprocess of Data Series of incomplete

Starting series .................................................... 6-24

6.3 Performing On-Line Data Processing ......................... 6-25

6.3.1 Printing of a Report ............................................ 6-25

6.3.2 Statistical Calculation ......................................... 6-25

6.3.3 Calibration .......................................................... 6-26

6.3.4 Blank Subtraction ............................................... 6-26

6.3.5 DAD Data Processing ........................................ 6-27

6.3.6 Data Processing for Two Detectors .................... 6-27

6.4 Quick Analysis Start ..................................................... 6-28

6.4.1 Setting Parameter .............................................. 6-28

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7. FUCTION AND OPERATION OF DATA-PROCESSING CONTROL WINDOW .............. 7-1

7.1 Configuration of Data-Processing Control Window .... 7-1

7.1.1 File Menu ............................................................. 7-2

7.1.2 Edit Menu ............................................................. 7-2

7.1.3 Option Menu......................................................... 7-3

7.1.4 Window Menu ...................................................... 7-4

7.1.5 Help Menu ............................................................ 7-4

7.2 Operation of Data-Processing Control Window ........... 7-5

7.2.1 Overview .............................................................. 7-5

7.2.2 Injection Table ...................................................... 7-5

7.2.3 Function Buttons .................................................. 7-6

7.3 Calibration Curve Window ........................................... 7-10

7.3.1 Overview ............................................................ 7-10

7.3.2 Options Menu ..................................................... 7-11

7.3.3 View Menu ......................................................... 7-13

7.3.4 Using Calibration Curves .................................... 7-14

7.4 Modify Report screen ................................................... 7-18

7.4.1 File Menu ........................................................... 7-18

7.4.2 Options Menu ..................................................... 7-19

7.4.3 Help Menu .......................................................... 7-20

7.5 Injection Table .............................................................. 7-21

7.5.1 Injection Table .................................................... 7-21

7.5.2 Recalculating...................................................... 7-22

8. DATA-PROCESSING CONTROL WINDOW -CHROMATOGRAM- ................................ 8-1

8.1 Configuration of Data-Processing control Window ..... 8-2

8.1.1 File Menu ............................................................. 8-3

8.1.2 Edit Menu ............................................................. 8-4

8.1.3 Data control Menu ................................................ 8-5

8.1.4 Options Menu ....................................................... 8-7

8.1.5 View Menu ........................................................... 8-9

8.1.6 Window Menu .................................................... 8-10

8.1.7 Manual Baseline Icons ....................................... 8-10

8.2 Setting up/Modifying Data Processing Parameters ... 8-11

8.2.1 Setting Up a Component Table with a

Chromatogram ................................................... 8-11

8.2.2 Setting Up Integration Time Table Using the

Displayed Chromatogram ................................... 8-12

8.2.3 Other Data Control Parameters .......................... 8-13

8.2.4 Manually Correcting Baselines ........................... 8-14

8.3 Using Multi-Display Mode ............................................ 8-16

8.3.1 Multi-Display window .......................................... 8-16

8.3.2 File Menu ........................................................... 8-17

8.3.3 Edit Menu ........................................................... 8-18

8.3.4 Option Menu....................................................... 8-19

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8.3.5 Window Menu .................................................... 8-26

8.3.6 Help Menu .......................................................... 8-26

8.4 Manipulating Display Features .................................... 8-27

8.4.1 Manipulating Display Features ........................... 8-27

8.4.2 Panning Graphs ................................................. 8-28

8.4.3 Changing the Upper Y-Axis Value ...................... 8-28

8.4.4 Moving the Line Cursor ...................................... 8-29

8.5 Include Statistics Report for QC or Unk Vials ............ 8-30

9. MODIFY REPORT ........................................................................................................... 9-1

9.1 Print Preview Window .................................................... 9-2

9.2 Opening the Report Preview Window ........................... 9-3

9.3 Functional Buttons ......................................................... 9-3

10. REPORT LAYOUT EDITOR .......................................................................................... 10-1

10.1 Outline for Creating Report ......................................... 10-1

10.2 Report Layout Editor .................................................... 10-3

10.2.1 Report Layout Editor Window ............................. 10-3

10.2.2 Template Display Area ....................................... 10-4

10.2.3 Separator ........................................................... 10-4

10.2.4 Flame ................................................................. 10-5

10.3 Menu Bar/Toolbar ......................................................... 10-6

10.3.1 File Menu ........................................................... 10-6

10.3.2 Edit Menu ........................................................... 10-7

10.3.3 Option Menu....................................................... 10-8

10.3.4 Window Menu .................................................... 10-8

10.3.5 Help Menu .......................................................... 10-9

10.4 Selection Buttons ....................................................... 10-10

10.4.1 Selection Buttons ............................................. 10-10

10.4.2 Report Items List .............................................. 10-12

10.4.3 Framed Report Items ....................................... 10-13

10.5 Report Layout Editor .................................................. 10-14

10.5.1 Generating a Report ......................................... 10-14

10.5.2 Mismatched Layout Items Dialog ..................... 10-15

10.6 Method Layout Template ........................................... 10-16

10.6.1 Creating a New Master Layout ......................... 10-16

10.6.2 Load Master Layout Dialog............................... 10-16

10.6.3 Basic Steps in Formatting a Layout .................. 10-17

10.7 Dependency Function of Report Items ..................... 10-34

10.7.1 Modifying Dependencies .................................. 10-34

10.7.2 Modify Dependencies Dialog ............................ 10-35

10.8 Master Layout ............................................................. 10-37

10.8.1 Modify Master Layout Dialog ............................ 10-37

10.8.2 Save Master Layout Dialog .............................. 10-38

10.9 Master Layout File ...................................................... 10-38

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10.10 Special Report Items .................................................. 10-39

10.10.1 Multi-Chrom Overlay Report ............................. 10-39

10.10.2 Resolution Report ............................................ 10-39

11. FUNCTIONS AND OPERATIONS OF MODULE

DETAILED INFORMATION WINDOW .......................................................................... 11-1

11.1 Status Display on Module Detailed Information

Window ......................................................................... 11-1

11.1.1 Common (system status) ................................... 11-1

11.1.2 1110 Pump ......................................................... 11-2

11.1.3 1210 autosampler .............................................. 11-2

11.1.4 1310 column oven .............................................. 11-3

11.1.5 1410 UV detector ............................................... 11-3

11.1.6 USB-AID ............................................................ 11-3

11.1.7 1430 DAD .......................................................... 11-4

11.2 Control Commands on Module Detailed Information

Window ......................................................................... 11-5

11.3 Status Display ............................................................... 11-8

11.3.1 1110 Pump Status Display ................................. 11-8

11.3.2 1210 Autosampler Status Display .................... 11-12

11.3.3 1310 Column Oven Status Display ................... 11-16

11.3.4 1410 UV Detector Status Display ..................... 11-17

11.3.5 USB-AID Status Display ................................... 11-20

11.3.6 1430 DAD Status Display ................................. 11-21

12. DATA RE-PROCESSING - 3D DATA - .......................................................................... 12-1

12.1 Composition of a data re-processing window ........... 12-2

12.1.1 File Menu ........................................................... 12-3

12.1.2 Edit Menu ........................................................... 12-4

12.1.3 Process Data Menu ............................................ 12-5

12.1.4 Options Menu ..................................................... 12-7

12.1.5 Library Menu .................................................... 12-11

12.1.6 View Menu ....................................................... 12-12

12.1.7 Window Menu .................................................. 12-15

12.2 Set and Modify Extracted Parameters of

Chromatogram ............................................................ 12-16

12.2.1 Display Chromatogram with

Arbitrary Wavelength ........................................ 12-16

12.2.2 Selecting/Displaying Chromatograms with

Fixed Wavelengths ........................................... 12-17

12.2.3 Displaying Integrated Chromatogram ............... 12-18

12.2.4 Displaying/Editing the Best Chromatogram ...... 12-19

12.2.5 Other Data Processing Parameter ................... 12-22

- vii -

12.2.6 Data Reprocessing of Extracted

Chromatogram ................................................. 12-23

12.3 Set and Modify Extracted Parameter of Spectrum ... 12-24

12.3.1 Spectrum Display of Arbitrary Time .................. 12-24

12.3.2 Overlapping Display of Spectrum ..................... 12-25

12.3.3 Selecting a Background-Spectrum/Displaying

a Background-Subtracted Spectrum ................ 12-26

12.3.4 Specify Integrated Spectrum ............................ 12-27

12.4 Performing/Displaying Peak-Purity Checks ............. 12-28

12.4.1 Perform Peak Purity Check .............................. 12-28

12.4.2 Check purity Check Spectrum .......................... 12-29

12.4.3 Set and modify Peak Purity Check Processing

Parameter ........................................................ 12-30

12.5 Other Display Window ............................................... 12-31

12.5.1 Chromatogram Display Window ....................... 12-31

12.5.2 Spectrum Display Window ............................... 12-32

12.5.3 Using 3-D Mesh View ....................................... 12-34

12.6 Change Operation of Graph View ............................. 12-36

12.6.1 Zooming and Un-zooming of Graph ................. 12-36

12.6.2 Moving the Chromatogram ............................... 12-37

12.6.3 changing Signal Intensity Scale ........................ 12-37

12.6.4 Moving Wavelength Cursor and Time Cursor ... 12-37

13. Spectrum Library Window .......................................................................................... 13-1

13.1 Configuration of Spectrum library Window ................ 13-1

13.1.1 Process Menu .................................................... 13-2

13.1.2 Options Menu ..................................................... 13-3

13.2 Managing Spectrum Library ........................................ 13-5

13.2.1 Modifying Library Spectrum Information ............. 13-5

13.2.2 Filtering of Spectrum List ................................... 13-8

13.2.3 Delete Spectrum ................................................ 13-9

13.3 Using Spectrum Library ............................................. 13-10

13.3.1 Library Menu .................................................... 13-12

13.3.2 Searching and Saving Spectrum Library .......... 13-14

13.3.3 Reverse Search library ..................................... 13-19

13.3.4 Search library under Data Acquisition .............. 13-21

14. HPLC APPLICATION INFORMATION .......................................................................... 14-1

14.1 Introduction .................................................................. 14-1

14.2 Example of a Solvent Time Table for

Gradient Elution ........................................................... 14-1

14.3 Determining a Sampling Period .................................. 14-2

14.3.1 Automatic Sampling Period Mode ...................... 14-2

14.3.2 Manual Sampling Period Mode........................... 14-2

14.4 Detector WL Table and Best WL Chromatogram ....... 14-3

- viii -

14.5 Quantification Methods ................................................ 14-3

14.5.1 Area% / Height% Method ................................... 14-3

14.5.2 Calibration Curve ............................................... 14-4

14.5.3 External Standard Method .................................. 14-5

14.5.4 Normalized-% Method ........................................ 14-7

14.5.5 Internal Standard Method ................................... 14-8

14.6 Peak Identification ...................................................... 14-10

14.6.1 Example of Percentage Time Window (%TIME)

......................................................................... 14-10

14.6.2 Example of Absolute Time Window

(Absolute Time) ................................................ 14-10

14.6.3 Peak Identification Rules .................................. 14-11

14.7 Blank Subtraction ....................................................... 14-12

14.8 Grouping Individual Components ............................. 14-14

14.9 Retention Time ........................................................... 14-15

14.9.1 Relative Retention Time (RRT) ........................ 14-15

14.9.2 Corrected Retention Time (CRT)...................... 14-16

14.10 Integration and Baseline Correction ......................... 14-18

14.10.1 Peak Detection and Integration ........................ 14-18

14.10.2 Noise ................................................................ 14-21

14.10.3 Bunching .......................................................... 14-22

14.10.4 Smoothing ........................................................ 14-22

14.10.5 Sensitivity ......................................................... 14-23

14.10.6 Peak Detection ................................................. 14-23

14.10.7 N-Method ......................................................... 14-23

14.10.8 Integration-Inhibit ............................................. 14-24

14.10.9 Tail ................................................................... 14-24

14.10.10 Vertical .......................................................... 14-24

14.10.11 Forward- and Backward-Horizontal ................ 14-24

14.10.12 Negative Peak ............................................... 14-25

14.10.13 Group ............................................................ 14-25

14.10.14 Precedence of Baseline Functions ................. 14-26

14.10.15 Baseline Code ............................................... 14-26

14.11 Peak Purity Check Using Spectra ............................. 14-28

14.12 Spectral Correlation in Spectral Peak ID, Peak Purity,

and Spectrum Library Search .................................... 14-30

14.13 Statistical Calculations Used by Primaide System

Manager ...................................................................... 14-31

14.13.1 Average ............................................................ 14-31

14.13.2 Dispersion (Variance) ....................................... 14-31

14.13.3 Standard Deviation ........................................... 14-31

14.13.4 Relative Standard Deviation ............................. 14-31

14.13.5 Coefficient of Determination ............................. 14-32

14.14 System Suitability Test (SST) .................................... 14-33

14.14.1 Capacity Factor, (k') ......................................... 14-34

- ix -

14.14.2 Selectivity, () .................................................. 14-34

14.14.3 Number of Theoretical Plates, N ...................... 14-35

14.14.4 Resolution, R.................................................... 14-37

14.14.5 Asymmetry, Asym ............................................ 14-39

14.14.6 Signal -to- Noise Ratio ..................................... 15-40

14.15 Data Diagnosis ........................................................... 14-42

14.15.1 Designating a Diagnosis Peak ......................... 14-42

14.15.2 Designating Expected Concentration (E-Conc)

Peaks ............................................................... 14-43

14.16 Decomposed Substance Report ................................ 14-44

14.16.1 Rule on Component Peak Search .................... 14-44

14.16.2 Calculation Method for Decomposed Substance

Report .............................................................. 14-45

14.17 Troubleshooting ......................................................... 14-46

14.17.1 Peak Determination Problems .......................... 14-46

14.17.2 Qualitative and Quantitative Analysis Problems14-49

14.17.3 Error Clearing Method ...................................... 14-50

APPENDIX .......................................................................................................... APPENDIX 1-1

APPENDIX 1. FUNCTION AND OPERATION OF

ONLINE DDE .................................... APPENDIX 1-1

AP.1.1 Operating Environment of Attached Online DDE

Program ............................................ APPENDIX 1-1

AP.1.2 Setting of Method .............................. APPENDIX 1-2

AP.1.3 Details of Attached Online DDE

Program ............................................ APPENDIX 1-3

AP.1.4 Setup of Online DDE ......................... APPENDIX 1-5

AP.1.5 Example of Online DDE Result ......... APPENDIX 1-6

APPENDIX 2. APPLICATION OF PRIMAIDE SYSTEM MANAGER

.......................................................... APPENDIX 2-1

AP.2.1 Application in Internal Standard

Method .............................................. APPENDIX 2-1

AP.2.2 Application of Report Output ............. APPENDIX 2-3

APPENDIX. 3 AIA FILE CONVERSIONS ................ APPENDIX 3-1

AP3.1 To Convert from Raw Data to

AIA Format ........................................ APPENDIX 3-1

APPENDIX. 4 CAUTION ON SETTING OF

SAMPLING PERIOD ......................... APPENDIX 4-1

APPENDIX. 5 CAUTION ON SETTING OF

RESPONSE ...................................... APPENDIX 5-1

APPENDIX. 6 ABOUT ERROR MESSAGE ............. APPENDIX 6-1

AP6.1 1110Pump A/B Message ................... APPENDIX 6-1

AP6.2 1210Autosampler .............................. APPENDIX 6-2

AP6.3 1310Column Oven ............................ APPENDIX 6-4

AP6.4 1410UV Detector ............................... APPENDIX 6-6

- x -

AP6.5 USB-AID ........................................... APPENDIX 6-7

AP6.6 1430 DAD ......................................... APPENDIX 6-8

TERMINOLOGY .............................................. TERMINOLOGY - 1

INDEX .............................................................................. INDEX - 1

1.1

1-1

1. OVERVIEW

CAUTION

Fatigue due to Long-Hour Operation

If you keep working with the display monitor and

keyboard for long hours, your eyes and body will be

fatigued to jeopardize your health. When working

with the display monitor for a long time, take a break

for 10 to 15 minutes per hour for health of your eyes

and body.

1.1 The Primaide System Manager The Primaide System Manager is supported the control function, the data

acquisition and the data processing function to a High Performance Liquid

Chromatography (HPLC) with the personal computer (PC).

The Primaide System Manager is linked through a USB interface board (IFB) to

control Primaide 1000 Series Pumps, autosampler, column oven, and UV

detector using Hitachi e-Line (elite Line).

3 D data acquisition and 3 D data processing from PC by the Primaide System

Manager become possible.

Primaide System Manager

HPLC System

Control

1.2 The Main Function

1-2

1.2 The Main Function 1) Method Development

Instrument-control parameters

Data-processing parameters

Report Layout Editor

2) Data Acquisition

On-line monitoring of HPLC units

On-line display of Chromatogram data

Multi-Method data acquisition

On-line monitor of 3 D contour map

Spectrum search library

3) Chromatogram Data Processing

Quantitation

System suitability testing

Identification of components by time window and spectra

Identifying the component by retention time and spectrum

4) 3 D Data Processing

Spectrum data processing

Contour map display and 3 D map display

Creating and searching the spectrum library

Comparing spectrum

Blank subtraction

Peak purity check

Extracting the chromatogram from 3 D data (DAD data)

5) Report Generation

On-line quantitation report

On-line confidence report

Reprocess-mode report generation

Export report files to Microsoft Excel and Microsoft Word

Data report of on-line DAD

6) File Management

Opening a Method, sample table, data, or report file

Saving a Method, sample table, data or report file

Copying files from one application to another

Deleting a Method, sample table, data, or report file

1.2

1-3

7) Module

Detailed information

Module control

Maintenance information/reset

2.

2-1

2. STARTING/EXITING THE PRIMAIDE SYSTEM MANAGER

2.1 Introduction The Primaide System Manager program uses the basic terminology and

techniques established for Windows operating system with regard to screen,

menu, mouse, keyboard, and Help functions.

Refer to the Microsoft Windows operating system User's Guide for a review of

these fundamentals.

NOTE: NOT RESPONDING message will be indicated on the screen of

Primaide System Manager when you execute a low-speed operation

such as a communication connection to an instrument. However,

software will not be affected. Please wait for a completion of

processing.

2.2 Starting/Exiting the Primaide System Manager

2-2

2.2 Starting/Exiting the Primaide System Manager Click [Primaide] icon on desktop.

The Primaide System Manager is started and the Main Window appears.

The Primaide System Manager clicks [All Program][Primaide][Primaide] of

the Start menu under the left of the window, Or, the Main Window is displayed

by starting when the [Primaide] icon of desktop is double-clicked.

Windows 7 Start Menu

(Primaide Icon)

Primaide System Manager is operated from the Main Window.

Chose [Exit] in File Menu of the Main Window for exiting the Primaide System

Manager. Or, please click button on the right of the Main Window.

2.3

2-3

2.3 Menu The main menu item is as follows.

2.3.1 File Menu

The File menu on the Primaide System Manager program contains the

following commands.

New Ctrl+N

Opens the New dialog box and creates a new Method or Sample Table.

Choose the New command from the File menu to open the New dialog box

shown below.

Highlight the desired file type (Method or Sample Table) and choose OK.

Either the Method Setup or Sample Setup starts with parameters set to

program defaults or blank settings.

You can modify parameters and choose Save As from the File menu to save the

file with a new name.

Open... Ctrl+O

Opens the Open File dialog box so you can open an existing Method, Sample

Table, Data Series, or Report.

Choose Open from the File menu to display the Open File dialog box shown

below:

2.3.1 File Menu

2-4

Refer to Table 2.1 for the functions of the Open File command buttons.

Table 2-1 Open File Commands

Button Function

Copy Opens the Copy dialog box to allow you to copy Methods,

Sample Tables, Data Series, and Reports.

Delete

The Delete button opens the Delete dialog box to delete files.

The Delete function is only available to certain users whom the

System Administrator has granted file deletion rights using the

Primaide Administration program.

Sort

Opens the Sort dialog box to sort Methods, Sample Tables,

Data, and Reports. The type of file being sorted is reflected in

the name visible in the Title Bar of the dialog box (e. g. , Sort

Sample). Files cannot be sorted by name or by date/time. The

sort order can be either ascending or descending.

Filter Initiates the Data Filter dialog box, but only when the chosen

File Type is Data.

Rename Opens the Rename dialog when the File Type is either Data or

Report. Use to change the file name or report name.

OK

Opens the highlighted file. If the File Type is Method, the

Method Information screen is opened. If it is Sample Tables,

the Setup Information screen is opened. If the File Type is

Data, the associated Injection Table is displayed. If the File

type is Report, the selected report is displayed in the Report

Preview window.

Cancel Cancels the Open File dialog box.

The Open File dialog box is accessible via the Method, Sample Table,

Data-Processing Control, and Preview/Print Report icons on the Main Tool Bar

in particular situations (See Section 3.2, Main Tool Bar Icons) Regardless of how

the dialog box is opened, you may select any file type.

2.3.1

2-5

Close

In general, choose Close from the File menu to close the window that is active.

The window can be for a Method, Sample Table, Injection Table, or data display.

In the following cases, however, the Close command is disabled by the Primaide

System Manager program:

(1) If one Method and one or more Injection Tables are open, the Primaide

System Manager program disables the Close command so that the Method

cannot be closed before the Injection Table.

(2) If one Sample Table is open and active, and the Data acquisition window is

also open, the Primaide System Manager program disables the Close

command so that the Sample Table that is currently in use for data

acquisition cannot be closed until the Data Acquisition window is closed.

When closing an Injection Table with the Close command, the Primaide System

Manager program automatically closes any associated data display windows

that are open.

Close All

Closes all open functions in all open windows regardless of focus. If any window

has been modified, the program offers the opportunity to save it.

When Data Acquisition is in a mode other than Idle Monitor, the Primaide

System Manager program closes all other types of windows except the Data

Acquisition window and the Sample Table that currently is in use.

If data acquisition is in Idle Monitor mode, the Primaide System Manager

program closes all windows.

Print Preview

The preview screen of the contents of the display can be displayed by clicking

menu bar. The print output can be performed from this screen.

Print Setup...

Lets you pick a printer, paper orientation (Portrait or Landscape), paper size

(letter, legal, A4, B5) and the paper source (tray or manual).

Print... Ctrl+P

The contents of the display can be printed.

Save Method

Saves the current Method on display in the Window. The previous version on

the hard disk is over-written after you respond to all Method validation

messages, if any.

2.3.1 File Menu

2-6

Save Method As...

The Save As dialog boxes allow you to specify the name of the current Method or

Sample Table and the name of the Application under which the Method or Table

is to be saved.

The Save As dialog box offers the following options:

a)Name

Enter a new name in the text-entry box or highlight an existing name displayed

in the list box.

b)Application

Highlight the application name in the list box to indicate where the Method or

Sample Table is to be saved.

c)Comment

Enter appropriate comments (99 characters, maximum) regarding the file saved

using the Save As function.

d)OK

Choose the OK button to save the Method or Sample Table with the specified

name to the specified application. If the name already exists in the application,

the Primaide System Manager program requests an OK to overwrite the

existing file.

e)Cancel

Choose the Cancel function to cancel the Save As dialog box.

2.3.2

2-7

Save Sample

Saves the Sample Table in focus.

When you select this command, the Primaide System Manager program starts

to validate the Sample Table and then initiate the Save As dialog box. The

previous file version on the hard disk is overwritten.

Save Sample As...

Initiates Sample Table validation and opens the Save As dialog box so that you

can specify the Title of the Sample Table and the name of the Application.

Exit

Exits the Primaide System Manager program.

Exit is inactive while you are acquiring data using Data Acquisition.

Prompts to save documents.

2.3.2 Edit Menu

The Edit menu on the Primaide System Manager program contains the

following commands.

Copy Ctrl+C

Copies a selection to the Windows clipboard. The original from which the

selection was copied remains unchanged. To transfer the selected (highlighted)

Method, Sample Table, Data or Report files from the current application to

another application, select (highlight) the application on the Copy dialog box.

Cut Ctrl+X

If it is a screen of the table, copies a highlighted item to the Windows clip-board,

and deletes the highlighted item.

Delete Ctrl+Del (table) , Del (Report edit)

If it is a screen of the table, deletes the highlighted item.

Paste Ctrl+V

Used in conjunction with the Cut and Copy commands.

Inserts a copy of the selection stored in the Windows clipboard at the insertion

point determined by the mouse pointer.

Edit Colors...

Opens the Color Selection dialog box to allow you to change screen colors. Items

associated with the current window are listed in the upper left box.

The Color Selection dialog box appears as shown below. Select an item and

change colors using the score bars. Click on the Preview button to preview the

new color; click on the Save button to save the color.

2.3.3 Window Menu

2-8

2.3.3 Window Menu


following commands.

Arrange Icons

Arrange icons at the bottom of the window.

Cascade

Arrange windows so they overlap.

Tile

Arrange windows as non-overlapping tiles.

2.3.4 Help Menu


following commands.

Index

List Help topics.

Using Help

Display instructions about how to use help.

About Primaide...

Displays the About the Primaide System Manager Program dialog box. This box

provides specific information such as the program name, part number, version

number, serial number, licensee name, and a copyright notice.

3.

3-1

3. PRIMAIDE SYSTEM MANAGER MAIN WINDOW Basic items on the Main window are shown below:

1) Title Bar

The title bar is the horizontal bar located along the top of the Main window. It

displays the program title (Primaide System Manager) and the names of the

current application (e. g. , samples, in the above illustration) and the current

Method.

2) Menu Bar

The menu bar is the horizontal bar located directly below the title bar. It lists

program menus.

The menus that are available change according to the task that is active. Each

menu, when opened, provides a list of command options. See a more detailed

description of the menus available to the Primaide System Manager.

3) Display Area

The display area is the large open area of the Primaide System Manager

program Main window. Initially, when the program boots up, the display area

shows the Primaide System Manager program logo. Subsequently, dependent

on the task selected, it displays one of a variety of task windows.

Menu Bar

Main Tool Bar

Status Bar

Display Area Title Bar

3. PRIMAIDE SYSTEM MANAGER MAIN WINDOW

3-2

4) Main Tool Bar

The Main Tool Bar is the vertical bar located along the left side of the main

window. It contains the following icons.

5) Status Bar

The status bar is the horizontal bar located along the bottom of the main

window. It displays program messages, current date and time, and item

information.

3.1

3-3

3.1 Menu Command Function of Main Window The menu of the main window is as follows.

3.1.1 File Menu

The following items are found in the File menu of the Main Window:

Please refer to [2.3.1 File Menu] for a general menu.

New Ctrl+N

Create a new document.

Open... Ctrl+O

Open an existing document.

Close All

Close all open documents.

Print Setup...

Change the printer and printing options.

Spectrum Library

The Spectrum Library window is opened. This command can be used in the

Main Window or the Reprocess Data window (See Chapter13 for library

window).

3.1.2 Help Menu

3-4

Exit

Quit the application; prompts to save documents.

3.1.2 Help Menu

The Help Menu contains the following commands. Index Opens a Help window

and displays a list of main topics.

Please refer to [2.3.4 Help Menu] for a general menu.

Index

List Help topics.

Using Help

Display instructions about how to use help.

About Primaide...

Display program information, version number and copyright.

3.2

3-5

3.2 Main Tool Bar Icons The Main Tool Bar Icons contains the followings.

Table 3-1 Main Tool Bar Icons

Icon Icon Name and Function

Change Application Select to open the Application dialog to switch to a different

application.

Method Setup Select to begin Method Setup functions.

Sampler Setup Select to begin Sample Table Setup functions.

Acquire Data Select to begin Data Acquisition functions.

Reprocess Data Select to begin Data Processing Control functions.

Report Select to open the Open File dialog box to review report

files.

System Status Select to open the Hardware Status dialog box to display

the current hardware status, before and after downloading

and during idle monitoring or data processing.

Pump(A/B) ON/OFF Select upper icon to toggle Pump A on/off; select lower

icon to toggle Pump B on/off.

Module Detailed

Information

Detailed information on the module (status) and the control

setting .

Quick Analysis Start It is possible to shift to the data collection monitor

promptly by the input of the analysis file name and the

rack name, etc.

3.2.1 Change Application Icon

3-6

3.2.1 Change Application Icon

Application feature to change from one system application to another.

When you click the Change Application icon, the Icon Primaide System

Manager program displays the Application dialog box.

In the list box, highlight the application name and choose Select.

(1) If one or more types of windows other than a Data Acquisition window are

open, the Primaide System Manager closes all the windows (i. e. , executes

Close All) and changes to the new application.

(2) If one or more types of windows including the Data Acquisition window are

open and data acquisition is in progress, the Primaide System Manager

closes all windows except the Data Acquisition window and the Sample

Table used by Data Acquisition, and changes to the new application.

(3) If one or more types of windows including a Data Acquisition window are

open and the Data acquisition window is in the Idle Monitor mode, the

Primaide System Manager closes all the windows (including the Data

Acquisition window and the Sample Table being used) and changes to the

new application.

(4) If nothing is open in the Main window, the Primaide System Manager

changes to the new application.

3.2.2 Method Setup Icon

Clicking on the Set Up Method icon initiates the Method Setup function.

The Primaide System Manager program requires that only one Method be open

at any time (except for reference Methods).

You cannot close a Method being used for data processing.

Depending on the situation, the Primaide System Manager program takes the

following actions when you choose the Method Setup icon:

(1) If no Method window is open (first-time log-in), the Primaide System

Manager program displays the Open File dialog box with Method selected

as the default File Type (i. e. , performs the Open command from the File

menu). The last-used Method for the current application is highlighted.

3.2.2

3-7

(2) If one Method window is open but not active (minimized to an icon), the

Primaide System Manager program minimizes all other windows to icons

and activates (maximizes) the open Method window.

(3) If one Method window is open and active and no Injection Table is open, the

Primaide System Manager program displays the Open File dialog box with

Method as a default File Type (i. e. , performs the Open command from the

File menu). The last-used Method for the current application is highlighted.

When you select a Method, a message appears for you to confirm Close

current Method(s)?. If Cancel is chosen, the Primaide System Manager

cancels the entire open file function. If OK is chosen, the Primaide System

Manager closes and saves the cur-rent Method and opens the new Method.

All other windows are minimized.

(4) If one Method is open and active, and one or more Injection Tables are open

as well, the Primaide System Manager displays the Open File Dialog with

Method as a default Type.

The last used Method for the current application is highlighted.

When you select a Method, the Primaide System Manager first verifies the new

Method configuration. (See Injection Table or Data Processing Tool)

If it does not match, an error dialog is displayed with detector types listed for

the new Method and the open Injection Tables and the Primaide System

Manager fails to open the new Method.

If the succeeds, a message appears for you to confirm Close current Method(s).

If Cancel is pressed, the Primaide System Manager cancels the entire open file

function.

If OK is pressed, the Primaide System Manager closes the current Method,

opens the new Method, and minimizes all other windows.

The Primaide System Manager then updates all open Injection Tables and their

graphical displays according to the newly opened Method.

3.2.3 Sampler Setup Icon

3-8

3.2.3 Sampler Setup Icon

You can open multiple Sample Tables via the Sample Table Setup Table Icon

function. However, it requires that one Sample Table window must be selected

when the Data Acquisition window is open.

Depending on the situation, the Primaide System Manager program takes one

of the following actions when you choose the Sampler Setup icon.

(1) If no Sample Table window is open, the Primaide System Manager program

displays the Open File dialog box with Sample Table chosen as the File

Type (i. e. , performs the Open command from the File menu).

The last-used Sample Table for the current application is highlighted.

When you select a Sample Table and choose OK, the Primaide System

Manager program opens the Sample Table and sets all other windows to

background.

(2) If one or more Sample Tables are open and all of them are minimized, the

Primaide System Manager program minimizes all other windows (e. g., a

Method window and restores (maximizes) all Sample Tables. 3. If one or

more Sample Tables are open and one is selected (active), the Primaide

System Manager program displays the Open File dialog box with Sample

Table selected as a default File Type (i. e., performs the Open command).

The last-used Sample Table for the current application is highlighted.

When you select a Sample Table and choose OK, the Primaide System

Manager program opens the Sample Table, maximizes all other Sample

Tables, and minimizes all other types of windows (e. g., a Method window).

3.2.4 Acquire Data Icon

The Primaide System Manager allows you to open one Data Acquisition

Window.

The following rules are followed when you click on the Acquire Data icon:

(1) If no Data Acquisition Window is open, the Primaide System Manager

displays the Open Sample Table for Data Acquisition Dialog where all

sample tables in the current application are listed.

Upon your selection of a Sample Table, the Primaide System Manager

opens the Sample Table and starts the Data Acquisition window by

validating the first Method in the Sample Table for starting Idle Monitor.

(2) If no Data Acquisition Window is open and no Sample Tables can be found

in the current application, the Primaide System Manager displays the

message: No sample tables available.

3.2.5

3-9

(3) If the Data Acquisition Window is already open but not active, the Primaide

System Manager restores (maximize) the Data Acquisition Window.

3.2.5 Reprocess Data Icon

A Method must be open before injection data can be opened via the

Data-Processing Control function.

The Primaide System Manager program takes the following actions when you

choose the Reprocess Data icon.

(1) If no Method is open and no Injection Table is open, the Primaide System

Manager program displays the Open File dialog box with Data as the

default File Type (i. e. , performs the Open command from the File menu).

The last-used or last-acquired Injection Table (whichever is more recent) for

the current application is highlighted.

When you select an Injection Table and choose OK, the Primaide System

Manager program automatically opens the Method used to acquire the

stored data.

It then proceeds to the Method verification.

(2) If one Method is open and one or more Injection Tables (including their

graphical display windows) are also open but not active, the Primaide

System Manager program restores all Injection Tables (including graphical

displays) with the last-opened one set to foreground and sets the Method to

background.

(3) If one Method is open, and one or more Injection Tables (including their

data display windows) are also open with one of them selected (active), the

Primaide System Manager program displays the Open File dialog box with

Data as the default File Type (i. e. , performs the Open command from the

File menu).

The last-used or last-acquired Injection Table (whichever is more recent) for

the current application is highlighted.

Upon your selection of an Injection Table, the Primaide System Manager

program verifies the Method configuration with the new Injection Table.

If it does not match, the Primaide System Manager displays the error

dialog indicating the detector types of the Method and the Injection Table

and fails to open the data.

If the Method verification is successful, it opens the Injection Table.

3.2.6 Report Icon

3-10

3.2.6 Report Icon

Choose the Preview/Print Report icon to have the Primaide System Manager

program perform the Open command and display the Open File dialog box with

Report chosen as a default.

The Open File dialog box contains a list of report files available from the data

files open on display.

If no file is open, the list appears blank.

NOTE: Before using Print Preview, ensure that the proper printer driver is

installed.

3.2.7

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3.2.7 System Status Icon

When you choose the System Status icon, the Module Status dialog box appears

showing the current system configuration.

After connection for communications with each module, the following icons can

be accessed.

Data Acquisition

Pump on/off

Module Detailed Information

Quick Analysis Start

It changes by the Primaide Administration program in the module composition.

You can change the configuration via the Primaide Administration program.

NOTE: The hardware components in the HPLC system must be set up using

the Primaide Administration program. If the program detects a

component that has not been set up in Primaide Administration, a

warning message appears, the component does not appear in the

screen display of system components, and the unconfigured

component is not considered a hardware component of the system.

3.2.7 System Status Icon

3-12

It explains the item of the Module Detailed Information dialog box as follows.

1) Status

Displays system status information that is also viewable in the Data

Acquisition window. When you first start the program, displays the message

"Downloading" while it downloads information to the Primaide System

Manager. Upon completion of the downloading process, the current system

status displays on screen.

2) Key Lock

The Lock/Unlock buttons are only activated for use by the System

Administrator.

Click on the respective button to Lock/Unlock instrument key. The Lock/Unlock

function over rides the setting on the GLP Options in the Primaide

Administration program.

3) Module

Interface

Displayed are the type of communication interface (namely, IFB), program No.

and serial No. that are recognized by the Primaide System Manager program.

When AID is connected, program No. will not be displayed.

Pump A

Pump A type of system, program No. and serial No. are displayed.

Pump B

Pump B type of system, program No. and serial No. are displayed.

Autosampler

The type of autosampler, program No. and serial No. are displayed.

Oven

The type of column oven, program No. and serial No. are displayed.

Detector Ch1/Ch2

The form, program No. , and cereal No. of the detector are shown.

Accessory

Connected attached device name or form (for instance, cooling unit) and

program No. and serial No. are shown.

4) OK

Click to the OK button to close the dialog box.

5) Initialize

Click to connect each module with Primaide System Manager. This allows the

interface module to recognize a new component that has been powered up after

the entire system was already operational.

6) Disconnection

Click to disable and disconnect from all data acquisition functions.

3.2.8

3-13

7) Error release

When the error occurs in connected module, the Error release button is

displayed.

NOTE: The added component must have already been configured as a part of

the system at some time in the past, using the Primaide

Administration program.

3.2.8 Pump(A) ON/OFF and Pump(B) On/Off Icon

When you choose the Pump(A/B) On/Off icon, the pump is turned on and off.

3.2.9 Module Detailed Information Icon

When you choose the Module Detailed Information icon, Open the Module

Detailed Information window.

The Module Detailed Information window is usable for checking the detailed

information about modules and controlling them.

3.2.10 Quick Analysis Start Icon

When quick analysis start icon of main tool bar is clicked, quick analysis

starting dialog is displayed.

4.

4-1

4. FUNCTION AND OPERATION OF METHOD WINDOW Method window contains the module operation parameters and the data

processing parameters. You can not set/edit a file by opening two or more

method files at the same time in a method window, but you can open a method

file that want to refer as the reference method window. You can copy all

parameters or a part of parameters of the reference method to the current

method. And the current method can be compared with the reference method.

4.1 Menu Command Function of Main Window Icons of the Method window are displayed two steps in the top of window.

1st step : Module operation parameters

2nd step: Data processor parameters (CH1, CH2)

4.1.1 File Menu

4-2

4.1.1 File Menu

The following items are found in the File menu of the Main Window:

New... Ctrl+N

Create a new document.

Open... Ctrl+O

Open an existing document.

Close

Close the active document.

Close All

Close all open documents.

Save Method Ctrl+S

Save the current Method.

Save Method As...

Save the current Method with a new name.

Print... Ctrl+P

Print the active document.

Print Preview

Display full pages.

Print Setup...

Change the printer and printing options.

Exit

Quit the application; prompts to save documents.

4.1.2 Edit Menu

The Edit menu allows you to perform a variety of editorial functions.

Please refer to [2.3.2 Edit Menu] for a general menu.

Cut Ctrl+X

Cut the selection and put it on the Clipboard.

Copy Ctrl+C

Copy the selection and put it on the Clipboard.

Paste Ctrl+V

Insert Clipboard contents.

Delete Ctrl+Del

Erase the selection.

Add New

Adds a new row after the last row on either the Component Table, the

Integration Time Table, and all other tables with corresponding default values.

4.1.3

4-3

Edit Colors

Opens the Color Selection dialog box to allow you to change screen colors. Items

associated with the current window are listed in the upper left box.

The Color Selection dialog box appears as shown below.

Select an item and change colors using the score bars. Click on the Preview

button to preview the new color; click on the Save button to save the color.

4.1.3 Module Setup Menu

The instrument condition menu on the Main screen of the Primaide System

Manager program contains the following commands.

Method Configuration

You can review or modify Method Configuration parameters from the Method

Configuration screen.

Method Information

You can review or modify general Method information such as the Method

History, Column Type, Method Name, and Solvent Names.

History is available only when the Method Log Book is selected in GLP options

when running the Primaide Administration program.

Pump Setup

The Interface Module, the Gradient Mode, and the number of solvents specified

for each Pump on the Method Configuration screen must be the same in both

Methods.

Autosampler Setup

Autosampler setup parameters window is displayed.

4.1.4 Data Process Setup Menu

4-4

Column Oven Setup

The oven selected on the Method Configuration must be the same in both

Methods.

Channel 1/2 Detector Setup

Channel 1/2 detector setup parameters window is displayed.

Time Summary

Displays the Pump (solvent) table and event table graphically.

4.1.4 Data Process Setup Menu

You can access the following capabilities of the Primaide System Manager

program using the Data Processing Setup menu:

Channel 1 (CH. 1) or Channel 2 (CH. 2)

Use the Channel 1/2 command to review or modify the data-processing method

(on all Data-Processing Control windows) used exclusively for analyzing data

acquired with the channel 1/2 detector. If a single detector is configured on the

Method Configuration screen, only the one command corresponding to the

configured detector is enabled (the other is disabled). For example, if Channel 1

is selected, Channel 2 is disabled and the Primaide System Manager program

displays data-processing parameters only for the Channel 1 detector.

Calculation Method

This command reviews or modifies the calculation method in the Calculation

Method screen.

Component Table

Sets up, reviews, or modifies the Component Table for data processing.

Concentration Table

Select this command or icon to review or set up the Concentration Table.

Coefficient Table

Reviews or modifies the coefficients of the calibration curve for each component.

4.1.5

4-5

Integration Table

Use this command to review or set up the Integration Time Table.

DAD Data Processing

Select to review or modify DAD Data Processing parameters.

Chromatogram Display Format

Use this command to open the Chromatogram Display Format Screen.

DAD Display Format

Use this command to open the DAD Display Format Screen.

Confidence Report

Use this command to set report parameters for System Suitability Tests,

Module Performance Tests, and Data Diagnosis in the Confidence Report

screen.

Report Format

Displays the Report Format dialog box.

Update Method

Click on this icon to apply the changes you have made to the current method to

open Data Display windows.

This icon is also available in the Component Table, and Integration Time Table

in the Data-Processing Control window.

4.1.5 Option Menu

The commands and features described in this section are available in the

Option menu.

Copy DP Channels...

Copies all data-processing parameters from channel 1 to channel of the current

Method.

You can enter an optional time offset that is added to

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