manual - epmotion 5075 (l, v, t, vt, m...5 table of contents epmotion® 5075 english (en) 7 labware...

nual075EN)e manual

Register your instrument! www.eppendorf.com/myeppendorf

epMotion® 5075Hardware manual

5075 900.840-02/032016

Copyright© 2016 Eppendorf AG, Germany. All rights reserved, including graphics and images. No part of

this publication may be reproduced without the prior permission of the copyright owner.

Qiagen® and Rotor-Disc® are registered trademarks of Qiagen GmbH, Germany.

epMotion®, Eppendorf Tubes ®, epT.I.P.S.®, and Eppendorf ThermoMixer® are registered trademarks of

Eppendorf AG, Germany.

Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

epMotion optical sensor U.S. Pat. No. 6,819,437

epMotion gripper U.S. Pat. No. 7,462,327

epMotion VAC sealing foil U.S. Pat. No. 6,666,978

U.S. Design Patents are listed on www.eppendorf.com/ip

The software of the device (firmware) contains open source software. License information is available on

request from Eppendorf AG, Germany.

Only for epMotion M5073, M5073c, 5073m, 5073mc, 5075m and 5075mc: Limited Use Label License

NOTICE TO PURCHASER; LIMITED LICENSE FOR RESEARCH USE ONLY.

This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The

purchase price for this product includes only limited, nontransferable rights under certain claims of certain

patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights

are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this

product for any commercial use. Further information regarding purchasing a license under patents of

Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for

commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development

Department, 10210 Genetic Center Drive, San Diego, California 92121-4362, U.S.A.

3Table of contentsepMotion® 5075

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

1.6 Revision history. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.1.1 Intended use of epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.5 Danger symbols and safety equipment on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213.1 Product overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.1.1 epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.1.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

3.2 MultiCon PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

3.3 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

3.3.1 epMotion 5075I and epMotion 5075lg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

3.3.2 epMotion 5075t and epMotion 5075tg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

3.3.3 epMotion 5075v and epMotion 5075vg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

3.3.4 epMotion 5075vt and epMotion 5075vtg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.3.5 epMotion 5075m and epMotion 5075mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.3.6 Accessories for all versions of the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

3.3.7 Accessories for epMotion with epBlue ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

3.3.8 Accessories for epMotion with epBlue GxP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

3.4 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

3.4.1 Mode of operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

3.4.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

3.4.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

3.4.4 Eppendorf ThermoMixer module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

3.4.5 Magnetic separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

3.4.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

3.4.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4.2 Installing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Table of contentsepMotion® 5075

English (EN)4

5 Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 315.1 Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.1.1 Design of the epMotion 5075I worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.1.2 Design of the epMotion 5075t worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

5.1.3 Design of the epMotion 5075v worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.1.4 Design of the epMotion 5075vt worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.1.5 Design of the epMotion 5075m worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.2 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.3.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.3.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.3.3 Equipping the thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5.4 Eppendorf ThermoMixer module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.4.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.4.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.4.3 Equipping the Eppendorf ThermoMixer module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.5 Magnetic finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.5.1 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.5.2 Equipping the magnetic finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.6.1 Vac Frame vacuum frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

5.6.2 Vac Lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

5.6.3 Silicone mat for Vac Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5.6.4 Vac Thermo Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5.6.5 Elution plate adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

5.6.6 Equipping the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

5.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.7.1 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.7.2 UV lamp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.8 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.9 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.9.1 Dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5.9.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

6 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 516.1 Procedure for dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

6.1.1 Functional principle of the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

6.1.2 Liquid aspiration up to the remaining volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

6.1.3 Bottom tolerance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

6.1.4 Transporting and dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.2 Dispensing modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.2.1 Dispensing mode: Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.2.2 Dispensing mode: Multidispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.2.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.3 Special features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.3.1 Liquid aspiration from base of vessel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.3.2 Liquid aspiration from high vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.3.3 Liquid dispensing at the height of the vessel edge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.3.4 Liquid aspiration from a filter plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59


English (EN)

7 Labware – vessels, plates and pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 617.1 Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.2 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

7.2.1 Deepwell plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.2.2 Microplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.2.3 Filter plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.2.4 Channeling plate 96 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

7.2.5 PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

7.2.6 Tube plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

7.3 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

7.3.1 Reservoir 30 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

7.3.2 Reservoir 100 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

7.3.3 Reservoir 400 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

7.4 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

7.4.1 epT.I.P.S. Motion Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

7.4.2 epT.I.P.S. Motion SafeRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

7.4.3 epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

7.4.4 TipHolder for epT.I.P.S. Motion Reloads. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

8 Labware accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 738.1 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

8.1.1 Rack for 24 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

8.1.2 Rack for 96 conical vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

8.1.3 0.5/1.5/2.0 mL Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

8.1.4 Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

8.1.5 PrepRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

8.1.6 ReagentRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

8.1.7 0.5/1.5/2.0 mL thermorack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

8.1.8 Thermorack TMX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

8.1.9 Thermorack rotor/tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

8.1.10 ReservoirRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

8.1.11 ReservoirRack 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

8.1.12 ReservoirRack modules. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.2 Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

8.2.1 Height adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

8.2.2 Vac Holder vacuum holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

8.2.3 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

8.2.4 Thermoadapter LC Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

8.2.5 Thermoadapter Microplate 96/V/U . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

8.2.6 Thermoadapter Frosty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

8.3 Thermoblock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

8.3.1 Thermoblock PCR 96 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

8.3.2 Thermoblock PCR 384 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

8.3.3 Thermoblock PCR 96 OC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

8.3.4 Thermoblock and thermoadapter comparison . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

8.4 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

8.4.1 Waste box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

8.4.2 LiquidWasteTub with lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92


English (EN)6

9 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 959.1 First steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

9.1.1 Switch on the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

9.1.2 Using the touch screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

9.1.3 Using the mouse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

9.1.4 Using the keyboard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

9.1.5 Backing up data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

9.1.6 Working with epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

9.2 Placing the tools and labware on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

9.2.1 Display epBlue worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

9.2.2 Placing dispensing tools on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

9.2.3 Placing the gripper on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

9.2.4 epT.I.P.S. Placing Motion on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

9.2.5 Placing the waste container on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

9.2.6 Placing the labware on the worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

9.3 Executing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

9.3.1 Carry out a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

9.3.2 Starting the application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

9.3.3 Controlling the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

9.4 Completing your work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

9.4.1 Cleaning up the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

9.4.2 Switch off epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10710.1 Decontamination and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

10.1.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

10.1.2 Decontamination methods and decontamination agents . . . . . . . . . . . . . . . . . . . . . . 108

10.1.3 Carrying out wipe decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

10.1.4 Carrying out the spray decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110

10.1.5 Carrying out the UV irradiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

10.1.6 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

10.3 Servicing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

10.4 Servicing the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

10.4.1 Replacing the seals of the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

10.4.2 Replacing the silicone mat of the Vac Lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

10.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

10.6 Performing a firmware update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

10.7 Checking the dispensing volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

10.8 Maintaining the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

10.8.1 Exchanging the sealing rings on the eight-channel dispensing tool . . . . . . . . . . . . . 115

10.9 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11711.1 Error search. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

11.2 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118

11.2.1 Optical sensor error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118

11.2.2 Dispensing error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118

11.2.3 Messages from epBlue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119


English (EN)

12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12112.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

12.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

13 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12313.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

13.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

13.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

13.4 Noise level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

13.5 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

13.6 MultiCon PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

13.7 Additional specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

13.7.1 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

13.7.2 Carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

13.7.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

13.8 Enhancements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125

13.8.1 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125

13.8.2 Eppendorf ThermoMixer module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125

13.8.3 Magnetic finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125

13.8.4 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125

13.8.5 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126

13.8.6 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126

13.8.7 UV lamp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126

13.9 Dispensing tool errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127

13.9.1 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127

13.9.2 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128

13.10 Test conditions for dispensing tool errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128

14 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12914.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129

14.2 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129

14.2.1 epT.I.P.S. Motion Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129

14.2.2 epT.I.P.S. Motion SafeRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130

14.2.3 epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130

14.2.4 Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131

14.3 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131

14.4 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132

14.5 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132

14.5.1 Racks for individual tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132

14.5.2 Thermoracks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133

14.5.3 ReservoirRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134

14.5.4 ReservoirRack modules. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134


English (EN)8

14.6 Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134

14.6.1 Height adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134

14.6.2 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135

14.7 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135

14.8 Accessories for the vacuum chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136

14.9 MagSep kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136

14.10 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136

14.11 Modification kits and enhancements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138

Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143

9Operating instructions

epMotion® 5075

English (EN)

1 Operating instructions1.1 Using this manual

Your epMotion operating manual consists of hardware instructions and software instructions. Separate

instructions exist for some optional software modules.

The operating manual is part of the product.

The current version of the operating manual can be found on our website: www.eppendorf.com.

Read the operating manual in full before using the device.

Store the operating manual at an easily accessible location.

The device may only be transferred with the operating manual.

If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.

1.2 Danger symbols and danger levels1.2.1 Danger symbols

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.2 Danger levels

Biohazard Explosive substances

Strong magnetic field UV radiation

Electric shock Heavy load

Cuts Hot surface

Hazard point Material damage

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

Operating instructionsepMotion® 5075

English (EN)10

1.3 Symbols used

1.4 Abbreviations used

BCRBarcode reader

CSVComma Separated Value

DNADeoxyribonucleic acid

DWPDeepwell plate

epT.I.P.S.Eppendorf Totally Integrated Pipetting System

HEPAHigh Efficiency Particulate Air (filter)

LHLiquid Handling

LIMSLaboratory Information Management System

MTPMicroplate

PCRPolymerase chain reaction

PDFPortable Document Format

TMXEppendorf ThermoMixer module

USBUniversal Serial Bus

UVUltraviolet radiation

VACVacuum unit

Depiction Meaning

1.

2.

Actions in the specified order

Actions without a specified order

• List

Text Display text or software text

Additional information


epMotion® 5075

English (EN)

1.5 Glossary

A

ApplicationProgram for a specific application. An application includes the procedure and the equipping of the

worktable.

B

Barcode readerDevice for scanning a barcode.

C

CleanCapOptional epMotion equipment. The CleanCap comprises a HEPA filter and at least one UV lamp.

D

Destination labwareRack or plate with destination positions.

Destination positionPosition in the labware that exclusively is destination of liquid transfers.

Dispensing toolTool for aspirating and dispensing liquids. Single-channel dispensing tools as well as eight-channel

dispensing tools for different volume ranges are available.

E

EasyCon TabletEppendorf EasyCon™ tablet. You can control your epMotion using the tablet and the epBlue software.

epBlueSoftware for creating and administering applications and labware. The epMotion is controlled by the

epBlue software.

Eppendorf QualityEppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the

requirements for standard products, e.g., precision, accuracy, wetting behavior and leak tightness.

epT.I.P.S. MotionPipette tips of the epMotion. epT.I.P.S.® Motions are available with or without a filter. The abbreviation

stands for Eppendorf Totally Integrated Pipetting System.

F

Filling volumeMaximum volume of a labware. The filling volume exceeds the working volume. The epMotion removes

liquid from the labware up to the filling volume.


English (EN)12

G

GripperTool for transporting the labware.

H

Height adapterAdapter, for low labware. The height differences between the labware will be offset to decrease the tool

holder paths, and therefore decrease the cycle time of the application.

HEPA filterAir filter. The filter prevents particles such as dust and germs from the environment entering the epMotion

workspace.

I

Intermediate labwareRack or plate with intermediate positions.

Intermediate positionPosition in the labware that is source and destination of liquid transfers.

L

LabwareRacks, plates, tips, etc. that are placed on the worktable.

LocationArea on the worktable for positioning labware.

M

MagSepSystem for nucleic acid purification with the epMotion. The MagSep consists of a magnetic finger module,

an Eppendorf ThermoMixer module, a PrepRack and reagents.

MultiCon PCA computer with touch screen. You can control your epMotion using the computer and the epBlue software.

P

PCR cleanPCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for

standard products, e.g., precision, accuracy, wetting behavior, leak tightness. PCR clean also meets the

requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors.

Consumables with the PCR clean purity grade are controlled and certified by an external laboratory.

Certificates are available from our webpage www.eppendorf.com.

ProcedureSequence of commands executed consecutively. Part of an application.


epMotion® 5075

English (EN)

R

RackMount for tubes or pipette tips.

Random errorPrecision, standard deviation. A measure for the scattering of the measured values around the average

value.

Remaining volumeVolume that cannot be aspirated from a vessel. Caused by the distance between the pipette tip and the

vessel base as defined in the software and the defined depth of immersion of the pipette tip in the liquid.

The pipette tip cannot aspirate the volume. The remaining volume depends on the tube geometry.

ReservoirReservoirs are used for preparing reagents. Reservoirs are hung in a ReservoirRack or placed directly on

the worktable.

S

SafeRackRack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination

of adjacent tips. If you want to use the tips several times, use SafeRacks.

Source labwareRack or plate with source positions.

Source positionPosition in a labware that exclusively is a source of liquid transfers.

SterileSterile is an Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard

products, e.g., precision, accuracy, wetting behavior, leak tightness. Sterile also meets the requirements

with regard to sterility and freedom from pyrogens.

Systematic errorInaccuracy. Deviation of the average value of the dispensed volumes from the selected volume.

T

Thermal moduleHeating plate for labware. The heating plate is integrated into the worktable.

ThermoadapterTemperature-controlled adapter that is not transported with the gripper. Plates are inserted into the

thermoadapter.

ThermoblockTemperature-controlled adapter that is transported with the gripper.

There are 2 types of thermoblocks:

• Thermoblocks: Thermoblocks, into which plates are inserted.

• Thermoblocks with plates Thermoblocks that are permanently connected with a plate.


English (EN)14

ThermorackTemperature-controlled rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.

V

Vacuum unitThe vacuum unit aspirates liquid from a filter plate by means of negative pressure. The vacuum unit

consists of a vacuum manifold, vacuum pump and vacuum frame.

W

Working volumeFilling volume minus volume of the immersing pipette tip. The epMotion fills the vessels up to the working

volume.

WorktableepMotion work surface that labware and tools are placed on. In the software, the epMotion worktable is

represented as epBlue worktable.

1.6 Revision history

Revision history of the epMotion 5075 hardware operating manual, order no. 5075 900.840.

Version of the operating manual Date Changes

Version 00 June 2013 Created new

Version 01 August 2015 Revision. New labware.

Version 02 March 2016 New certificate.

15Safety

epMotion® 5075

English (EN)

2 Safety2.1 Intended use2.1.1 Intended use of epMotion 5075

The device is intended for use in laboratories for research, development, and industrial and routine work,

as well as for training purposes. The areas of applications include – but are not limited to – the fields of life

sciences, biotechnology, chemistry and clinical research.

All epMotion 5075 (l, t, v, vt, m) automated pipetting systems are designed for monitoring the

contamination-free, accurate and correct dispensing and transferring of liquids and for controlling

automatically the combination of liquids.

The autoclavable dispensing tools work in a volume range of 1 μL to 1000 μL.

The epMotion 5075v with integrated vacuum station for example performs a fully automatic nucleic acid

purification.

The epMotion 5075m with integrated Eppendorf ThermoMixer module is used for applications during

which the vessels must not be closed when mixing and incubating, and it performs a fully automatic nucleic

acid purification with magnetic particles.

The epMotion 5075t with integrated Eppendorf ThermoMixer module is used for applications during which

the vessels may be open when mixing and incubating.

The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the

declaration of conformity, but the product is not registered with the FDA.

These automated pipetting systems are exclusively intended for use indoors and may only be used by

skilled specialists who have received adequate training.

2.2 User profile

This device may only be operated by specialists.

The specialists must have received training on this device. The training must have been conducted by

Eppendorf AG or an authorized partner of Eppendorf AG.

The specialists must have read thoroughly the operating manual of the device. The specialists must have

read thoroughly the operating manuals of all software modules.

SafetyepMotion® 5075

English (EN)16

2.3 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any

resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

2.4 Warnings for intended use

DANGER! Risk of explosion.

Do not operate the device in areas where work is completed with explosive substances.

Do not use this device to process any explosive or highly reactive substances.

Do not use this device to process any substances which could create an explosive

atmosphere.

DANGER! Damage to health due to escaping aerosols.When working with harmful substances, dangerous aerosols can occur and escape at the

vacuum pump.

Contact Eppendorf AG before using these types of substances.

WARNING! Lethal voltages inside the device.Touching parts which are under high voltage may cause an electric shock. An electric shock

injures the heart and causes respiratory paralysis.

Ensure that the housing is closed and undamaged.

Do not remove the housing.

Ensure that no liquid can penetrate into the device.

Only authorized service staff may open the device.

WARNING! Electric shock due to damage to device or mains cable.

Only switch on the device if the device and mains cable are undamaged.

Only use devices that have been properly installed or repaired.

In case of danger, disconnect the device from the mains supply. Disconnect the mains/

power plug from the device or the earth/grounded socket. Use the designated isolating

device (e.g., emergency switch in the lab).

17Safety

epMotion® 5075

English (EN)

WARNING! Risk from incorrect supply voltage

Only connect the device to voltage sources which correspond to the electrical

requirements on the name plate.

Only use sockets with a protective earth (PE) conductor and suitable mains/power cord.

WARNING! Damage to health due to infectious liquids and pathogenic germs.

When handling infectious liquids and pathogenic germs, observe the national regulations,

the biological security level of your laboratory, the Material Safety Data Sheets, and the

manufacturer's application notes.

Wear your personal protective equipment.

For comprehensive regulations about handling germs or biological material of risk group II

or higher, please refer to the "Laboratory Biosafety Manual" (source: World Health

Organization, Laboratory Biosafety Manual, in its respectively current valid version).

WARNING! Health hazard from skin contact with infectious substances.

Wear protective gloves.

WARNING! Danger due to flammable and infectious liquids in the waste container.Pipette tips in the waste container may contain flammable or infectious liquids.


Handle the pipette tips and sample materials from the waste container in accordance with

the Material Safety Data Sheets, safety regulations and laboratory guidelines.

WARNING! Damage to health due to contaminated device and accessories.

Decontaminate the device and the accessories before storage and dispatch.

CAUTION! Risk of injury due to the movement of the carrier.The carrier may still be moving when front hood of the epMotion is opened.

Wait until the carrier is standing still before reaching into the device.

CAUTION! Cuts due to broken glass.A damaged touch screen leads to cuts on the hands.

Only work with the touch screen if it is not damaged.

CAUTION! Poor safety due to incorrect accessories and spare parts.The use of accessories and spare parts other than those recommended by Eppendorf may

impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or

accept any liability for damage resulting from the use of incorrect or non-recommended

accessories and spare parts, or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.


English (EN)18

2.5 Danger symbols and safety equipment on the device

This section explains the warning symbols on the epMotion and the location of the safety equipment.

Abb. 2-1: Danger symbols on epMotion 5075

Fig. 2-1: Danger symbols on epMotion 5075

NOTICE! Spilled liquid can cause damage to the device.

Switch off the device.

Disconnect the mains/power plug.

Collect the spilled liquid. Observe the specifications for the liquid in the Material Safety

Data Sheets.

NOTICE! Disposable products (single-use items) change in size through autoclaving.

Do not use autoclaved disposable products in automated applications.

epMotion U5075epMotion 5075

6

5

1 2

3 4

19Safety

epMotion® 5075

English (EN)

Tab. 2-1: Danger symbols

Tab. 2-2: Safety device

1 WARNINGObserve the operating manual.

2 WARNINGThe carrier may continue moving after the front panel has

been opened.

Wait until the carrier stops moving before reaching into

the device.

3 DANGERRisk of burns from the hot surface.

Wait until the surface has cooled before touching it.

5 NOTICEDanger due to UV radiation.


6 The label is located on the magnetic finger module and is

only visible to the authorized service.

WARNINGDanger due to strong magnetic field.

Do not work with the device if you have a cardiac

pacemaker.

4 The housing protects the user from UV radiation, magnetic

fields, contamination and movable parts.


English (EN)20

21Product description

epMotion® 5075

English (EN)

3 Product description3.1 Product overview

The epMotion 5075 is available with various features. Details of the worktable for your device (see

Worktable on p. 31).

3.1.1 epMotion 5075Abb. 3-1: Main illustration epMotion 5075I

Fig. 3-1: Main illustration epMotion 5075I

1 CarrierThe carrier moves in X-direction, Y-direction, and

Z-direction.

2 Tool holderHolds dispensing tools.

3 Optical sensorDetects levels, tips, and labware.

4 CleanCapContains HEPA filter and UV lamps

5 WorktableWork surface for tools and labware.

6 Front hoodSafety device for protection from movable parts

and contamination.

7 Thermal module (optional)Plate for heating and cooling labware.

8 Mains/power switch

9 Mouse

10 MultiCon PCControl device used for controlling the epMotion.

epMotion 5075

1 42 3

5910 678

x

y

z

Product descriptionepMotion® 5075

English (EN)22

3.1.2 Interfaces

Abb. 3-2: epMotion 5075 interfaces

Fig. 3-2: epMotion 5075 interfaces

Only devices which meet the prerequisites of IEC 950/EN 60950-1 (UL 1950) standards may be connected

to the interfaces.

1 EthernetConnection for the cable to the MultiCon PC

2 USBPort for a USB storage medium for firmware

updates.

3 Mains/power connection

1 2

3


epMotion® 5075

English (EN)

3.2 MultiCon PC

Abb. 3-3: MultiCon PC

Fig. 3-3: MultiCon PC

The MultiCon PC is used for controlling the epMotion. The MultiCon PC has a touch screen.

Information on operation and the interfaces can be found in the accompanying manufacturer's original

instructions.


English (EN)24

3.3 Delivery package

The epMotion is available in various versions. Your device has been equipped according to your

specifications. For some software modules additional accessories are available.

3.3.1 epMotion 5075I and epMotion 5075lg

3.3.2 epMotion 5075t and epMotion 5075tg

3.3.3 epMotion 5075v and epMotion 5075vg

Quantity Order no. (International)

Description

Automatic liquid handling system epMotion 5075lmain device; with MultiCon PC, keyboard, mouse, installed software

epBlue

1 5075 000.271 100 V - 240 V, 50 / 60 Hz

Automatic liquid handling system epMotion 5075lgmain device; with MultiCon PC, keyboard, mouse, installed software

epBlue GxP

or 1 5075 000.275 100 V - 240 V, 50 / 60 Hz


Description

Automatic liquid handling system epMotion 5075tmain device with Eppendorf ThermoMixer module; with MultiCon PC,

keyboard, mouse, installed software epBlue

1 5075 000.272 100 V - 240 V, 50 / 60 Hz

Automatic liquid handling system epMotion 5075tgmain device with Eppendorf ThermoMixer module; with MultiCon PC,

keyboard, mouse, installed software epBlue GxP

or 1 5075 000.276 100 V - 240 V, 50 / 60 Hz


Description

Automatic liquid handling system epMotion 5075vmain device with vacuum unit; with MultiCon PC, keyboard, mouse,

installed software epBlue

1 5075 000.273 100 V - 240 V, 50 / 60 Hz

Automatic liquid handling system epMotion 5075vgmain device with vacuum unit; with MultiCon PC, keyboard, mouse,

installed software epBlue GxP

or 1 5075 000.277 100 V - 240 V, 50 / 60 Hz

1 – 400 mL reservoirVac Frame 2

1 5075 785.005 vacuum frame

Vac Holder1 5075 778.009 adapter for Vac Frame

1 5282 000.018 Gripper


epMotion® 5075

English (EN)

3.3.4 epMotion 5075vt and epMotion 5075vtg

3.3.5 epMotion 5075m and epMotion 5075mg


Description

Automatic liquid handling system epMotion 5075vtmain device with vacuum unit and Eppendorf ThermoMixer module;

with MultiCon PC, keyboard, mouse, installed software epBlue

1 5075 000.274 100 V - 240 V, 50 / 60 Hz

Automatic liquid handling system epMotion 5075vtgmain device with vacuum item and Eppendorf ThermoMixer module;

with MultiCon PC, keyboard, mouse, installed software epBlue GxP

or 1 5075 000.278 100 V - 240 V, 50 / 60 Hz

1 – 400 mL reservoirVac Frame 2

1 5075 785.005 vacuum frame

Vac Holder1 5075 778.009 adapter for Vac Frame

1 5282 000.018 Gripper


Description

Automatic liquid handling system epMotion 5075mmain device with magnet finger module and Eppendorf ThermoMixer

module; with MultiCon PC, keyboard, mouse, installed software epBlue

1 5075 000.270 100 V - 240 V, 50 / 60 Hz

Automatic liquid handling system epMotion 5075mgmain device with magnet finger module and Eppendorf ThermoMixer

module; with MultiCon PC, keyboard, mouse, installed software epBlue

GxP

or 1 5075 000.279 100 V - 240 V, 50 / 60 Hz


English (EN)26

3.3.6 Accessories for all versions of the epMotion

3.3.7 Accessories for epMotion with epBlue ID

3.3.8 Accessories for epMotion with epBlue GxP


Description

Mouse1 – With USB connecting cable

Keyboard1 – With USB connecting cable

Cable1 – For connecting the control device to the epMotion

Mains/power cord1 – Consistent with the country of order or country of destination

USB Ethernet adapter1 – For connecting the computer to a network

1 – Tool for transport securing deviceWaste box

1 5075 753.006 for used pipette tips

Operating Manual 1 5075 900.840 Hardware-manual epMotion 5075

1 5075 900.866 Software operating manual epBlue with MultiCon PC

CleanCapoptional 1 5075 751.607 with HEPA filter and UV-lamp

UV lampoptional 2 – For epMotion with CleanCap


Description

1 Software epBlue IDBarcode reader

1 With USB cable and table foot

Installation Guide1 5075 003.350 epBlue ID


Description

1 epBlue GxP softwareDocumentation folder

1 – Folder for certificates and documentation

Installation Guide1 5075 002.353 epBlue GxP

Operating Manual 5075 900.874 epBlue GxP


epMotion® 5075

English (EN)

3.4 Features

The epMotion 5075 is available with different features. All available features are described in this manual.

3.4.1 Mode of operation

The epMotion 5075 is an automatic device dispensing liquids by means of dispensing tools and pipette tips.

The epMotion 5075 dispenses liquids in the volume range 1 μL – 1 000 μL.

The dispensing tool aspirates liquid from source positions and dispenses this liquid in destination

positions. The dispensing tool works according to the piston-stroke principle. For the epMotion, dispensing

tools are available for 3 volume ranges.

The epMotion 5075 features an optical sensor. The sensor checks:

• Type and position of labware

• Number and positions of the ep.T.I.P.S. pipette tips. Motion pipette tips in TipHolders and Racks by

Eppendorf.

• Filling level of vessels

The epMotion 5075 is operated with the MultiCon PC.

The epMotion 5075 is controlled by the epBlue software. You can use the epBlue software to define

dispensing processes and compile them into an application. To do so, you select the source positions and

destination positions, determine the procedure and define the pattern.

When working with the MultiCon PC, a user administration feature is available.

3.4.2 Gripper

A gripper is available for the epMotion 5075. The gripper stacks and transports labware.

3.4.3 Thermal module

The epMotion 5075 can be equipped with a maximum of 3 thermal modules.


English (EN)28

3.4.4 Eppendorf ThermoMixer module

3.4.5 Magnetic separation

MagSep reagent kits are available from Eppendorf for this system.

3.4.6 Vacuum unit

The epMotion 5075 can be equipped with a vacuum unit.

Thus, the epMotion 5075 realizes a fully automatic nucleic acid purification.

3.4.7 CleanCap

The epMotion 5075 can be equipped with a CleanCap. The CleanCap contains a UV lamp and HEPA filter.

For further information on your epMotion, please go to the webpage www.eppendorf.com/

automation.

29Installation

epMotion® 5075

English (EN)

4 Installation4.1 Selecting the location

Information on ambient conditions, dimensions, and weights can be found in the technical data (see

Weight/dimensions on p. 123).

Select the device location according to the following criteria:

Electrical connections• mains/power connection as on the name plate.

• The mains/power switch and cutting unit of the power system circuit (e.g., FI protective switch) can be

accessed during operation.

Location• The ambient conditions match the specifications in the technical data.

• The location is well ventilated.

• The location is protected from direct sunlight.

• The location is not next to heat sources, such as heaters or drier compartments.

• There is enough space available for the device. The minimum distances to other devices and walls is

6 cm.

• The device can be safely and easily operated at this location.

Workplace• The lab bench is permanently mounted.

• The lab bench is designed for the weight of the device.

• The lab bench has a horizontal, plane work surface.

• The lab bench has a non-slip surface.

• The lab bench is vibration-free.

• There are no vibrating devices on the lab bench.

4.2 Installing the device

Information on transport (see Transport on p. 121).

The epMotion may only be installed and commissioned by service technicians authorized by

Eppendorf.

InstallationepMotion® 5075

English (EN)30

31Hardware

epMotion® 5075

English (EN)

5 Hardware5.1 Worktable5.1.1 Design of the epMotion 5075I worktableAbb. 5-1: epMotion 5075I worktable

Fig. 5-1: epMotion 5075I worktable

5.1.2 Design of the epMotion 5075t worktableAbb. 5-2: epMotion 5075t worktable

Fig. 5-2: epMotion 5075t worktable

A2-C5Locations for labware

C1-C3With up to 3 thermal modules as an optional feature

T1-T4Locations for dispensing tools

T0With a gripper as an optional feature

WasteLocation for waste container


C1-C2With 2 thermal modules as an optional feature

A4Eppendorf ThermoMixer module




TM 1 TM 2 TM 3Waste

A2T0 T1

T4T2

T3 A3 A4 A5

B3 B4 B5B2B1B0

C1 C2 C3 C4 C5

TM 1 TM 2

TMX

Waste

A2T0 T1

T4T2

T3 A3A4 A5

B3 B4 B5B2B1B0

C1 C2 C3 C4 C5

HardwareepMotion® 5075

English (EN)32

5.1.3 Design of the epMotion 5075v worktable

Abb. 5-3: epMotion 5075v worktable

Fig. 5-3: epMotion 5075v worktable

5.1.4 Design of the epMotion 5075vt worktable

Abb. 5-4: epMotion 5075vt worktable

Fig. 5-4: epMotion 5075vt worktable


C1-C3With 3 thermal modules as an optional feature

B4Vacuum unit


T0Location for gripper





B4Vacuum unit


T0Location for gripper


TM 1 TM 2 TM 3

VAC

Waste

A2T0 T1

T4T2

T3 A3 A4

B4B3B2B1B0

C1 C2 C3 C4

TM 1 TM 2

TMX

Waste

A2T0 T1

T4T2

T3 A3A4

B3B2B1B0

C1 C2 C3 C4

VAC

B4

33Hardware

epMotion® 5075

English (EN)

5.1.5 Design of the epMotion 5075m worktable

Abb. 5-5: epMotion 5075m worktable

Fig. 5-5: epMotion 5075m worktable




MagSepMagnet finger module




TM 1 TM 2Waste

A2T0 T1

T4T2

T3 A3A4 A5

B3 B4 B5B2B1B0

C1 C2 C3 C4 C5

TMX

MagSep


English (EN)34

5.2 Optical sensor

Abb. 5-6: Optical sensor principle

Fig. 5-6: Optical sensor principle

On the carrier, the optical sensor is located to the right of the tool holder.

The optical sensor records the intensity of the reflected light using a lateral infrared light source,

semi-transparent mirror, fixed lens and the movement in the z-direction. Height z0 is defined as the point

with the highest intensity which enables the detection of the level in a vessel or the presence of labware.

The optical sensor can detect horizontal, plane surfaces. The surface of the liquid must be at an angle of

90° ± 3° to the optical axis.

The surfaces of liquids can be strongly bent by the vessel geometry or the physical properties of the liquid

or vessel. The optical sensor may not detect the level on strongly bent surfaces. If this is the case, the user

must enter the liquid volume.

The optical sensor has a detection limit for vessel levels. The detection limit depends on the vessel

geometry. Generally, levels of 3 mm or higher can be detected.

DetectorDiaphragm

Z

Z0

Optics

Light source

Semi-transparentmirror

Diaphragm

35Hardware

epMotion® 5075

English (EN)

5.3 Thermal module

Depending on the model, your epMotion can be equipped with one or more thermal modules.

5.3.1 Safety notes

The optical sensor has the following functions:

Measure filling level in vessels• The optical sensor determines the filling level in the position of the labware. There are 2 ways of doing

this:

– The optical sensor determines the filling level in all positions used.

– The optical sensor determines the filling level in the first position, in the last position and in 8

randomly determined positions.

– The optical sensor cannot measure the filling level in the following labware: 384-well plates, Qiagen

rotor disc 72, Qiagen rotor disc 100.

Detect pipette tips• The optical sensor determines the quantity and the positions of the ep.T.I.P.S. pipette tips. Motion

pipette tips in TipHolders and Racks by Eppendorf.

Check labware on the worktable• The optical sensor scans the code on the labware.

• epBlue checks if the labware on the epMotion worktable matches the labware on the epBlue worktable.

Information on setting the optical sensor, and a detailed description of all functions, can be

found in the software operating manual.

WARNING! Danger due to flammable or explosive liquids.

Do not use explosive substances.

Do not heat up highly flammable substances.

Only heat up easily flammable substances in small amounts below the boiling point.

Do not exceed the boiling point of solutions.

CAUTION! Burns from hot thermal module.The thermal module heats up during operation. The software displays the current temperature

of the thermal module.

Do not touch the hot thermal module.

Wait until the thermal module has cooled down.

CAUTION! Risk of burns on hot labware.Labware is tempered during operation.

Do not touch tempered labware.

Wait until the labware has completely cooled off.


English (EN)36

5.3.2 ConfigurationAbb. 5-7: Thermal module

Fig. 5-7: Thermal module

Thermal modules temper liquids in a temperature range of 0 °C – 110°C.

The following specifications were determined an ambient temperature of 25 °C. The values mere measured

in the edge positions of the labware. First the thermal module was brought to the target temperature. Then

the labware was placed on the thermal module.

Tab. 5-1: Temperature control duration for labware with thermal module

NOTICE! Material damage due to non-heat-resistant labware.If you do not use heat-resistant labware, the samples and labware will get damaged.

Observe the labware specifications.

Only use labware made of metal, polypropylene (PP) or polycarbonate (PC) at set

temperatures over 100 °C.

Labware on the thermal module

Water filling volume per well in µL

Cooling from 25°C to 4°C

Heating from 25°C to 37°C


Temperature setting in °C

min Temperature setting in °C


min

Thermal module without

labware

5 2 8

Thermoadapter with

96-well twin.tec

PCR plate skirted

50 3 7 38 5 104 11

Thermoblock with 96-well

twin.tec PCR plate skirted

50 3 6 37 4 101 10

Thermoadapter with

384-well twin.tec

PCR plate skirted

10 3 5 37 5 103 8

Thermoblock with 384-well

twin.tec PCR plate skirted

10 3 5 37 5 98 7

37Hardware

epMotion® 5075

English (EN)

5.3.3 Equipping the thermal module

If you are using a thermoblock or thermoadapter, plates with uneven bases will be optimally tempered, e.g.

PCR plates.

Equip the thermal module as follows:

1. Place the plate on the thermoadapter or thermoblock.

2. Place the plate, with thermoadapter or thermoblock, on the thermal module.

24-unit thermorack with

0.5 mL Safe-Lock tubes

350 3 24 37 16 101 35



1200 3 30 38 18 104 44



1700 3 25 37 17 100 40

Information on setting the thermal module and a detailed description of all functions can be

found in the "Operation" chapter of the software instructions.

Labware on the thermal module

Water filling volume per well in µL

Cooling from 25°C to 4°C



Temperature setting in °C



min


English (EN)38

5.4 Eppendorf ThermoMixer module

Depending on the model, your epMotion can be equipped with an Eppendorf ThermoMixer module.

5.4.1 Safety notes

WARNING! Danger due to flammable or explosive liquids.

Do not use explosive substances.

Do not heat up highly flammable substances.

Only heat up easily flammable substances in small amounts below the boiling point.

Do not exceed the boiling point of solutions.

CAUTION! Risk of burns on the hot Eppendorf ThermoMixer module.The Eppendorf ThermoMixer module gets hot during operation. The software displays the

current temperature of the Eppendorf ThermoMixer module.

Do not touch the hot Eppendorf ThermoMixer module.

Wait until the Eppendorf ThermoMixer module has cooled down.

CAUTION! Risk of burns on hot labware.Labware is tempered during operation.

Do not touch tempered labware.

Wait until the labware has completely cooled off.

NOTICE! Contamination of samples and the device due to excessive speeds.If the speed is set too high, liquid will spray out of the vessels. Labware may become loose

from the holder and fly around.

Observe the labware maximum speeds.

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5.4.2 Configuration

Abb. 5-8: Eppendorf ThermoMixer module

Fig. 5-8: Eppendorf ThermoMixer module

The Eppendorf ThermoMixer module tempers and mixes liquids and suspensions.

The Eppendorf ThermoMixer module works in a temperature range of 4 °C – 95°C. The lowest temperature

the Eppendorf ThermoMixer module reaches is 15 °C below ambient temperature. If the ambient

temperature is 20 °C, the Eppendorf ThermoMixer module reaches a minimum of 5 °C.

Tab. 5-2: Maximum speed for labware on the Eppendorf ThermoMixer module

Labware Maximum speed in rpm

Vessels 1000

Deepwell plates with 96 wells 1200

Deepwell plates with 384 wells 1200

Microplate with 6 wells 1000

Microplates with 96 wells 2000

Microplates with 384 wells 2000

PCR plate with 96 wells 2000

PCR plate with 384 wells 2000

ReservoirRack Mixing not possible

Thermorack or Thermoblock 1000

Thermoblock 96 with a semi-skirted PCR plate for 250 μL 1700

Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/V 1600

Thermoadapter for Microplate 96/U/V with Eppendorf Microplate 96/U 1600

Thermoblock PCR 96 OC 1700

Thermoadapter for 96 wells 1000

Thermoadapter for 384 wells 1000

Thermorack TMX 1300

PrepRack 1300

Information on setting the Eppendorf ThermoMixer module, and a detailed description of all

functions, can be found in the software instructions.


English (EN)40

5.4.3 Equipping the Eppendorf ThermoMixer module

If you are using a thermoblock or thermoadapter, plates with uneven bases will be optimally tempered, e.g.

PCR plates.

Equip the Eppendorf ThermoMixer module as follows:

1. Place the plate on the thermoadapter or thermoblock.

2. Place the plate, with thermoadapter or thermoblock, on the Eppendorf ThermoMixer module.

The plate will be fastened to the Eppendorf ThermoMixer module during the application.

5.5 Magnetic finger module

Depending on the model, your epMotion can be equipped with a magnetic finger module.

5.5.1 Configuration

Abb. 5-9: Magnetic finger module

Fig. 5-9: Magnetic finger module

The magnetic finger module is located behind the Eppendorf ThermoMixer module.

The magnetic finger module is used for isolating suspended magnetic particles.

The magnetic finger module has 3 fingers that can be moved horizontally. Strong permanent magnets are

located on each finger. The 3 magnetic fingers move toward the vessels via the lateral openings in the

PrepRack. The magnetic field separates the magnetic particles on the tube inner wall. The dispensing tool

can remove liquid from the vessels, or place liquid in the vessels, while the magnetic particles are held in

place.

The PrepRack is required to perform a separation (see p. 75).

The separation duration can be defined using the software.

For a complete nucleic acid preparation, you can use the MagSep kits by Eppendorf AG. You can use

magnetic particles from other manufacturers for other applications. Additional information on this

procedure can be found in the software operating manual.

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5.5.2 Equipping the magnetic finger module

Equip the magnetic finger module as follows:

1. Equip the PrepRack with vessels.

2. Place the equipped PrepRack on the Eppendorf ThermoMixer module.

The PrepRack will be attached on the Eppendorf ThermoMixer module when the application is started.

5.6 Vacuum unit

Abb. 5-10: Vacuum unit with vacuum frame

Fig. 5-10: Vacuum unit with vacuum frame

The vacuum unit consists of a vacuum chamber, vacuum pump, and vacuum frame and a lid.

The vacuum pump creates a vacuum in the vacuum chamber. The vacuum sucks liquid from a filter plate

into a collection vessel. The strength of the vessel is configured in the software.

You can use the following labware as collection vessels:• Microplate

• Deepwell plate

• Tube plate

• Reservoir 400 mL


The gripper places filter plates, Vac Lid, and Vac Thermo Lid on the vacuum frame. The gripper transports

the vacuum frame with the attached filter plate.

The geometry of the used vessels is important for the quality of the separation. Use 2.0 mL

Safe-Lock tubes.


English (EN)42

5.6.1 Vac Frame vacuum frame

Abb. 5-11: Vac Frame 1

Fig. 5-11: Vac Frame 1

The vacuum frame is placed on the vacuum unit. The filter plate is positioned on the vacuum frame.

In order to produce a vacuum, the frame of the plate must be lying on the seal of the vacuum frame. Two

different heights of vacuum frame are available for this purpose.

The vacuum frame is positioned on the vacuum unit or on the vacuum holder. The vacuum frame cannot be

positioned directly on the worktable.

The vacuum frame is transported with the gripper.

5.6.2 Vac Lid

Abb. 5-12: Vac Lid

Fig. 5-12: Vac Lid

If you do not want to apply the vacuum to the entire plate, use the Vac Lid. The Vac Lid contains 96 bores.

The bores correspond to the positions of the wells on the filter plate.

The Vac Lid is positioned on the vacuum unit or on the gripper holder.

The Vac Lid is transported with the gripper.

1. Place the Vac Lid on the vacuum unit.

2. Cover any bores that are not required with the silicone mat.

1 Vac Lid

2 Silicone mat

3 Pins

1

2

3

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5.6.3 Silicone mat for Vac Lid

Abb. 5-13: Bores in the Vac Lid covered with silicone mat

Fig. 5-13: Bores in the Vac Lid covered with silicone mat

If you are not using some of the filter plate wells, cover the corresponding bores in the Vac Lid with a

silicone mat. The air does not flow through the covered bores. The vacuum only affects the wells that are in

use.

Silicone mats are available in different sizes. You can cover 16, 32, 48, 64 or 80 bores with the silicone mat.

The silicone mat has the number of bores it covers punched into it.

To attach a silicone mat on the Vac Lid, place the silicone mat on the pins of the Vac Lid.

5.6.4 Vac Thermo Lid

Abb. 5-14: Vac Thermo Lid

Fig. 5-14: Vac Thermo Lid

The Vac Thermo Lid has a block with a high heat capacity. The Vac Thermo Lid is heated on a thermal

module. On the vacuum unit, air is sucked in through the slits in the Vac Thermo Lid. The air heats up on

the block and flows through the filter plates.

Use the Vac Thermo Lid to optimize automated nucleic acid preparation. You can minimize the alcohol in

filter plates, the risk of contamination from alcohol is reduced.

The Vac Thermo Lid can be placed on a thermal module and on the vacuum unit.

The gripper transports the Vac Thermo Lid.

The Vac Thermo Lid cannot be placed on the Eppendorf ThermoMixer module.


English (EN)44

5.6.4.1 Using the Vac Thermo Lid

1. Place the Vac Thermo Lid on a thermal module.

2. Heat the Vac Thermo Lid to the required temperature before starting the application.

Heating the Vac Thermo Lid to 110 °C takes approx. 30 min.

5.6.5 Elution plate adapter

Abb. 5-15: Elution plate adapter

Fig. 5-15: Elution plate adapter

If you are using tube plates as collection vessels you need to use the elution plate adapter. The elution plate

adapter ensures that the tube plates sit firmly in one position.

The elution plate adapter is made from stainless steel.

5.6.5.1 Using the elution plate adapter

Place elution plate adapter on the edge of the tube plate.

CAUTION! Risk of burns on the hot underside of the Vac Thermo Lid.When the Vac Thermo Lid is tempered, the underside gets hot.

Wait until the underside of the Vac Thermo Lid has completely cooled off.

Do not place a ReservoirRack with reservoirs in the transport route of the Vac Thermo Lid.

ReservoirRacks with reservoirs collide with the Vac Thermo Lid on the gripper.

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5.6.6 Equipping the vacuum unit

Load the vacuum unit as follows:

1. Place the collection vessel on the vacuum unit.

2. If applicable, place channeling plate 96 on the collection vessel.

3. Place the vacuum frame on the collection vessel.

The weight of the vacuum frame presses the collection vessel into the vacuum unit.

4. Place the filter plate in the vacuum frame.

5. If applicable, place the Vac Lid or the Vac Thermo Lid on the filter plate.

The label on the Vac Lid and the Vac Thermo Lid must face the front.

6. The Vac Lid and Vac Thermo Lid can be transported during the application. For the transport, place the

Vac Lid on the gripper holder and the Vac Thermo Lid on a thermal module.

1 Vac Lid

2 Filter plate

3 Vacuum frame

4 Collection vessel

5 Vacuum unit

If the vacuum frame is incorrectly positioned, the vacuum will not be generated.

1

2

3

4

5


English (EN)46

5.7 CleanCap

The CleanCap is an optional piece of equipment of the epMotion and can only be ordered in combination

with a new device. The CleanCap includes a HEPA filter and a UV lamp.

5.7.1 HEPA filter

The HEPA filter cleans the air in the epMotion workspace. The HEPA filter reduces the amount of particles

such as dust or germs.

The fan continuously draws air out of the surroundings and channels it through the HEPA filter. The filtered

airflow will be routed to the epMotion workspace.

5.7.1.1 Using HEPA filters

Use the HEPA filter as follows:

Prerequisites

• epMotion is ready for operation.

1. Decontaminate the worktable and the lateral surfaces in the workspace of the epMotion.

2. Decontaminate labware and dispensing tools.

3. Close the front hood.

4. Switch on the HEPA filter using the epBlue software.

5. Let the HEPA filter run for 5 min.

All of the air in the epMotion workspace has been filtered.

6. Load epMotion.


8. Start the application.

The HEPA filter remains switched on after the end of the application.

9. Start another application, or switch off the HEPA filter using the software.

WARNING! Risk of infection due to aerosols.If you work with the HEPA filter, a continuous airflow will be blown from the device workspace

to the environment. The airflow can transport aerosols and contaminate the surrounding area.

Do not use the HEPA filter when working with biohazardous substances.

Disinfect contaminated surfaces in the workspace before switching on the HEPA filter.

The HEPA filter becomes dirty over time. Have the HEPA filter replaced by the authorized

service partner once per year.

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5.7.2 UV lamp

The UV lamp decontaminates the worktable. The UV irradiation lasts 15 min. The UV lamp is controlled by

the software.

5.7.2.1 Using UV lamps

Prerequisites


1. Remove the labware, waste container and dispensing tools from the worktable.


3. Switch on the UV lamp using the software (refer to the epMotion software manual).

The UV lamp switches off when the front hood is opened.

CAUTION! Risk to health due to UV radiation.UV radiation damages the eyes and skin.

UV radiation may escape outward through the ventilation slits in the rear panel of the device.

Wear your personal protective equipment when you are near the ventilation slits.

NOTICE! UV radiation causes damage to plastics.Plastics become brittle due to UV radiation exposure.

Remove the dispensing tools, labware and waste container from the worktable before

switching on the UV lamp.

The radiant flux of the UV lamp decreases with use. Have the UV lamp replaced by an

authorized service partner once every 2 years.

Information on decontaminating labware and dispensing tools is available (see

Decontamination and cleaning on p. 107)


English (EN)48

5.8 Barcode reader

Abb. 5-16: Model of a barcode reader

Fig. 5-16: Model of a barcode reader

With the barcode reader you can track and document your samples via IDs.

The barcode reader is connected to a USB port on the .

Plates have an ID. The ID of a well is generated automatically from the ID of the plate and the identification

of the well. Racks do not have an ID. Each tube in a rack has an ID.

The barcode reader has 2 settings:• Manual: To read an ID, press the key on the barcode reader.

• Automatic: To read an ID, hold the barcode reader to the ID. The barcode reader detects the ID

automatically.

5.9 Tools

The tool holder holds a dispensing tool and releases it. The tool holder recognizes the tool by its gold

contacts.

The tool holder also holds the gripper.

1 Window 2 Key

NOTICE! Damage to gold contacts due to handling.If you damage or contaminate the gold contacts on the tool, then the tool is damaged.

Do not touch the gold contacts.

1

2

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5.9.1 Dispensing tools

Abb. 5-17: Single-channel dispensing tool and eight-channel dispensing tool

Fig. 5-17: Single-channel dispensing tool and eight-channel dispensing tool

The dispensing tools are piston-stroke pipettes. The function of the dispensing tools is described in the

chapter (see Dispensing liquids on p. 51).

Single-channel dispensing tools and eight-channel dispensing tools, with 3 volume ranges each, are

available for the epMotion.

Tab. 5-3: Volume range of the dispensing tools

The dispensing tool errors can be found in the technical data (see p. 127).

Help on dispensing errors can be found in the "Maintenance" chapter (see Dispensing error on p. 118)

Single-channel dispensing tool

Eight-channel dispensing tool Volume range

TS 50 TM 50 1 μL – 50 μL

TS 300 TM 300 20 μL – 300 μL

TS 1000 TM 1000 40 μL – 1000 μL

5050


English (EN)50

5.9.2 Gripper

Abb. 5-18: Gripper with gripper holder

Fig. 5-18: Gripper with gripper holder

The gripper transports labware. The gripper uses the mandrels on the gripper arms to hold the labware.

The gripper is put down on the gripper holder.

CAUTION! Risk of injury through spikes on the gripper arms.Each gripper arm has 2 sharp spikes on the inner side.

Hold the gripper from above.

Do not hold the gripper arms on the inside.

The gripper transports the following labware: The gripper does not transport the following labware:

• Plate

Skirted and semi-skirted PCR plates are

transported. Plates can be placed on the

worktable, on other PCR plates, on a height

adapter or thermoadapter.

• Thermoblock

Thermoblocks are transported without plate or

with the positioned plate. The positioned plate

will not be picked up by the gripper without the

thermoblock.

Thermoblocks with plates are transported with

the firmly attached plate.

• Thermorack

Thermoracks are transported with the inserted

reaction vessels. The inserted reaction vessels

will not be picked up by the gripper without the

thermorack.

• PrepRack

Will be transported with the inserted reaction

vessels. The inserted reaction vessels will not

be picked up by the gripper without the

thermorack.


• Vacuum frame

Transported alone or carrying a filter plate.

• Vac Lid and Vac Thermo Lid

• Individual vessels

• Unskirted PCR plates

• Stacked plates

Only the uppermost plate is transported from

stacked plates.

• epT.I.P.S. Motion Rack

• Rack for 24 vessels

• Rack for 96 conical vessels

• Thermorack Rotor/Tubes

• ReservoirRack

• Reservoirs and ReservoirRack modules

• Height adapter

• Thermoadapter

Only the plate will be transported if it is inserted in a

thermoadapter.

• ReagentRack

51Dispensing liquids

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6 Dispensing liquids6.1 Procedure for dispensing6.1.1 Functional principle of the dispensing tools

Abb. 6-1: Piston-stroke pipette principle

Fig. 6-1: Piston-stroke pipette principle

The dispensing tools are piston-stroke pipettes that function according to the air cushion principle.

If the piston moves upward in the dispensing tool, liquid will be aspirated into the pipette tip. If the piston

moves downward in the dispensing tool, liquid will be dispensed from the pipette tip. The pistons will be

moved by a motor in the tool holder.

In eight-channel dispensing tools, all 8 pistons move simultaneously.

A Aspiration of volumeThe piston moves upward from the starting

position to the upper end position. As it moves

up, the piston aspirates volume.

B Dispensing volumeDispensing mode: Pipette The piston travels to

the starting position in one step.

Dispensing mode: Multidispense The piston

travels to the starting position in several steps.

As it moves, the piston dispenses volume.

C Dispensing the remaining volumeThe remaining volume is dispensed by a blow-out

stroke.

A B C

upper end position

basic positionend of blow-out

Dispensing liquidsepMotion® 5075

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6.1.2 Liquid aspiration up to the remaining volume

Abb. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth

Fig. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth

The pipette tip is immersed 3 mm into the liquid before liquid aspiration. The pipette tip moves downward

during liquid aspiration. The immersion depth of 3 mm remains constant while the liquid level decreases.

If the pipette tip reaches a distance of 1 mm to the base of the vessel, the pipette tip reduces its immersion

depth to 0.7 mm. If the liquid level continues to sink, the pipette tip will not aspirate any additional liquid.

The remaining volume remains in the vessel.

A remaining volume is present because the pipette tip has a minimum immersion depth in the liquid and a

minimum distance to the base of the vessel. Under normal conditions, the remaining volume is calculated

using a filling level of 1.7 mm.

The distance of the pipette tip to the base of the vessel can be changed in the software (refer to the

epMotion software manual).

6.1.3 Bottom tolerance

The bottom tolerance is the distance between the vessel base and the lower end of the pipette tip. The

default setting for the bottom tolerance is 1 mm. For 30 mL and 100 mL reservoirs, the default setting for

the bottom tolerance is 2.5 mm.

Decreasing the bottom tolerance also reduces the remaining volume.

1. If you remove a liquid from above a pellet, increase the bottom tolerance.

2. Do not decrease the bottom tolerance on uneven plates.

3. Check the set bottom tolerance when exchanging pipette tips, plates or vessels.

If the liquid is aspirated up to the remaining volume, the curvature of the liquid level can lead

to errors when dispensing.

1 mm0,7 mm


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6.1.4 Transporting and dispensing liquid

Abb. 6-3: Transporting and dispensing liquid

Fig. 6-3: Transporting and dispensing liquid

6.1.4.1 Dispensing liquid

The liquid in the pipette tip will be dispensed via free jet dispensing. The liquid will be dispensed into the

vessel 3 mm – 4 mm above the surface of the liquid. The pipette tip moves upward during dispensing. The

distance from the pipette tip to the surface of the liquid remains constant.

For the liquid type Protein C, a distance of 5 mm from the pipette tip to the surface of the liquid is set in the

software. This setting takes into account the tendency of concentrated protein solutions to form foam.

6.1.4.2 Transporting liquids

Before the transport, liquid is retracted into the pipette tip. An area filled with air is created in the lower

part of the pipette tip. The liquid does not drip during transport.

A Dispensing liquid B Retraction of liquid after liquid aspiration

~ 3 - 4 mm ~ 6 - 8 mm

A B


English (EN)54

6.2 Dispensing modes

The epMotion can be used to dispense liquids in the Pipette and Multidispense modes.

6.2.1 Dispensing mode: Pipette

In the Pipette mode, a defined volume is aspirated and fully dispensed in one step.

In order to aspirate the volume, the piston moves from the starting position to the upper end position.

In order to release the volume, the piston moves from the upper end position to the starting position in one

step.

When the piston is in the starting position, there is still residual volume in the pipette tip. The piston

performs a blow-out stroke and releases the residual volume at the destination position . The residual

volume is part of the dispensing volume. You can configure the blow-out stroke in the software using the

Initial stroke function, (refer to the epMotion software manual).

In the Pipette mode, the smallest dispensing volume for water is 1 μL.

Dispensing errors are smaller than in the mode Multidispense (see Dispensing tool errors on p. 127).

6.2.2 Dispensing mode: Multidispense

In the Multidispense mode, a volume is aspirated and fully dispensed in defined steps.

In order to aspirate the volume, the piston moves from the starting position to the upper end position. More

volume is aspirated than is needed for the dispensing step.

The piston must now be moved to a defined position. To do this, the piston performs a reverse stroke and is

moved to the end position. Part of the aspirated volume is returned to the source position.

After the reverse stroke, the dispensing steps are carried out. The piston moves from the upper end

position to the starting position in defined steps and releases the volume.

When the piston is in the starting position, there is still residual volume in the pipette tip. The piston

performs a remaining stroke and releases the residual volume.

In Multidispense mode the smallest volume for a dispensing step with water is 3 μL.

Dispensing errors are larger than in the Pipette mode (see Dispensing tool errors on p. 127).


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6.2.2.1 Volume aspiration in the Multidispense dispensing mode

Reverse stroke• Is performed after volume aspiration.

• Moves the piston to a defined position.

• During this movement, part of the aspirated volume is returned to the source position.

The reverse stroke is the same size for all liquids.

Remaining stroke• After dispensing, a residual volume remains in the pipette tip. This residual volume is released by the

remaining stroke after dispensing.

If the pipette tips are not replaced before each liquid aspiration, the residual volume is released into the

source position.

If the pipette tips are replaced before each liquid aspiration, the residual volume is released into the

waste container.

The required volume depends on the dispensing tool. Die epBlue software automatically calculates the

volume.

6.2.2.2 Example of volume aspiration in the Multidispense dispensing mode

A 96-well plate is to be filled with 10 μL water per position by dispensing. The eight-channel dispensing

tool TM 50 will be used. The volume is extracted from a reservoir. The pipette tips are not replaced before a

new volume aspiration.

Sum of the volumes for dispensing:• 96 × 10 μL for 96-well plate: 960 μL

• 8 × 5.8 μL Reverse stroke: 46.4 μL

• 8 × 2.5 μL Remaining stroke: 20 μL

• Sum: 1026.4 µL

Dispensing tool Volume per channel for reverse stroke

Volume per channel for remaining stroke

Single-channel dispensing tool TS 50

Eight-channel dispensing tool TM 50

5,8 μL 2.5 μL

Single-channel dispensing tool TS 300 16,7 μL 3,7 μL

Eight-channel dispensing tool TM 300 45,2 μL 5,0 μL

Single-channel dispensing tool TS 1000

Eight-channel dispensing tool TM 1000

50.3 μL 35.2 μL


English (EN)56

6.2.3 Mixing

Liquids can be mixed via multiple pipetting.

A mixing cycle consists of one upward movement and one downward movement of the piston in the

dispensing tool.

The aspiration level and dispensing level of the liquid can be defined for mixing. A defined height should

only be used for levels below the working volume as the vessel may overflow with larger levels.

During the mixing process, the pipette tip is immersed in the liquid. The blow-out is performed after

mixing above the liquid surface.

Defined mixing frequencies are defined in the liquid types. When using your own settings, determine the

optimal mixing frequency via experiments.

6.2.3.1 Recommended mixing frequencies

You can set mixing frequencies of 0.2 mm/s – 110 mm/s.

NOTICE! Contamination of the dispensing tool due to excessive mixing speed.If the mixing speed is too high, liquid can get into the dispensing tool.

If the liquid has a low viscosity or tends to foam, set lower mixing speeds.

Test the mixing settings using demineralized water.

Use pipette tips with filter.

Dispensing tool Mixing frequency of lower volume range in mm/s

Mixing frequency of medium volume range in mm/s

Mixing frequency of upper volume range in mm/s

TS 50 15 – 88 15 – 44 10 – 40

TM 50 15 – 88 15 – 44 10 – 40

TS 300 5 – 15 6 – 16 6 – 16

TM 300 2 – 11 2 – 11 2 – 11

TS 1000 4 – 15 4 – 15 4 – 15

TM 1000 4 – 15 4 – 15 4 – 15


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6.3 Special features6.3.1 Liquid aspiration from base of vessel

Optionally, the pipette tip can also be positioned above the base of the vessel at the height of the bottom

tolerance. This prevents the pipette tip from changing its position during liquid aspiration.

The filling level in the vessels may not exceed the working volume. If the filling level exceeds the working

volume, liquid may overflow.

Liquid aspiration from the base of the vessel is recommended in the following cases:

• For vessels ≤0.5 mL, 96-well plates and 384-well plates

• For plates with various filling volumes in the wells

• For vessels with low filling volumes

Liquid aspiration from the base of the vessel can be activated in the software using the Aspirate from bottom

option (refer to the epMotion software manual). The bottom tolerance depends on the labware. You can set

the bottom tolerance in the software.

Abb. 6-4: Displacement of the liquid in vessels

Fig. 6-4: Displacement of the liquid in vessels

6.3.2 Liquid aspiration from high vessels

Observe the following special features when using high vessels, e.g. 15 mL conical vessels.

A Vessel filled up to the working volume B Vessel with maximally immersed tip before liquid aspiration

A B


English (EN)58

6.3.2.1 Liquid aspiration from base of vessel

Abb. 6-5: Large vessel with high filling level

Fig. 6-5: Large vessel with high filling level

If you use vessels > 3 mL with high filling levels, do not aspirate the volume from the base of the vessel. The

high filling level will wet the outside of the pipette tip. This increases the risk of contamination and distorts

the dispensing result. For very large vessels with high levels, the entire pipette tip and the tip cone of the

dispensing tool will be wetted.

6.3.2.2 High remaining volume

Abb. 6-6: Liquid aspiration from high vessels with 1000 μL, 50 μL and 300 μL pipette tips

Fig. 6-6: Liquid aspiration from high vessels with 1000 μL, 50 μL and 300 μL pipette tips

50 μL tips and 300 μL tips do not extend as far into the vessels as 1000 μL tips. In high vessels, 50 μL and

300 μL tips do not reach the base of the vessels. If you use 50 μL and 300 μL tips, the remaining volume

will be larger than with 1000 μL tips.

Work with the 1000 μL dispensing tool when you use high vessels.

If you use the 50 μL dispensing tool or the 300 μL dispensing tool, and would like to reduce

the remaining volume, use lower vessels.


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6.3.3 Liquid dispensing at the height of the vessel edge

In the software, the pipette tip can be positioned at a height of 3 mm – 4 mm below the edge of the vessel

using the Dispense from top option. The height of the pipette tip does not change.

Use the option for:

• Quick dispensing.

• Dispensing in vessels with small diameters, e.g. 384-well plates, capillaries.

The tip remains in the upper area of the vessel and does not move further into the vessel. This virtually

eliminates the possibility of the pipette tip becoming contaminated.

• Dispensing in plates with vastly different filling levels in the wells.

6.3.4 Liquid aspiration from a filter plate

In the software, liquid can be suctioned from the supernatant of filter plates and filter vessels via the Elution

from filter option.

The Elution from filter option refers to the source position and almost fully aspirates liquid from the filter

plate. The pipette tip touches the filter. The piston movement in the dispensing tool for liquid aspiration

starts when the pipette tip moves into the vessel. The maximum stroke is used for each dispensing tool.

This also applies to dispensing.

When the liquid is being transported, air bubbles may be present at several locations in the pipette tip. The

size of the air bubble on the lower end of the pipette tip may vary.


English (EN)60

61Labware – vessels, plates and pipette tips

epMotion® 5075

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7 Labware – vessels, plates and pipette tips

The term labware refers to epMotion consumables and accessories. Consumables are vessels, plates and

pipette tips. Accessories are adapters, blocks and racks. epBlue includes a definition for labware that is

placed on the epMotion worktable. The definition includes data on the geometry, tempering ability, volume

and bottom tolerance of the labware.

Abb. 7-1: Reaction vessel with lid

Fig. 7-1: Reaction vessel with lid

7.1 Tubes

Tubes are inserted into racks. All tubes which fit in an epMotion rack can be used.

Tubes that can be inserted into a rack:

• Safe-Lock tubes

• Standard reaction vessels 3810X

• PCR tubes

• Conical tubes/centrifuge tubes

• Cryo tubes

Individual tubes are not transported with the gripper.

The level measurement with the optical sensor is more difficult with 0.2 mL and 0.5 mL tubes.

Use thermoracks or ReservoirRack modules if you want to temper tubes.

The epMotion can be used with a variety of labware from various manufacturers. Information

on how to expand the labware library can be found in the software operating manual.

Labware may not exceed an overall height of 126 mm. If this overall height is exceeded, the

software generates an error message.

1 Filling volume

2 Work volume

3 Detection limit of optical sensor

4 Remaining volume

1.5

1.0

0.5

0.1

1

2

34

Labware – vessels, plates and pipette tipsepMotion® 5075

English (EN)62

7.2 Plates

Abb. 7-2: Plates: left 96-well Microplate, center 24-well Microplate, right 96-well Deepwell plate

Fig. 7-2: Plates: left 96-well Microplate, center 24-well Microplate, right 96-well Deepwell plate

The following plates are available for the epMotion:

• Deepwell plates with 24, 96 or 384 wells

• Microplates with 6, 24, 48, 96 or 384 wells

• Filter plates for use with the vacuum unit

• PCR plates with 96 or 384 wells

• Plates with 96 individual vessels (tube plates)

Skirted plates can be placed directly at a worktable location. Skirted, semi-skirted and unskirted plates can

be placed on adapters.

Skirted and semi-skirted PCR plates are transported with the gripper. Unskirted plates are not transported

with the gripper.

The upper plate is transported from stacked plates.

Stacking plates

You can stack plates of the same type on the worktable.

For stacking, only use plates by Eppendorf.

Plates from other manufacturers may have different dimensions.


epMotion® 5075

English (EN)

7.2.1 Deepwell plates

Abb. 7-3: Deepwell plate with 96 wells

Fig. 7-3: Deepwell plate with 96 wells

A maximum of 2 Eppendorf Deepwell Plates can be stacked at one location.

A thermoadapter is available for Eppendorf 96/1000 μL Deepwell Plates. A thermoblock is available for

Eppendorf 96/2000 μL Deepwell Plates.

7.2.2 Microplates

Abb. 7-4: Microplate with 96 wells

Fig. 7-4: Microplate with 96 wells

A maximum of 5 Eppendorf Microplates can be stacked at one location.

A level measurement using the optical sensor is not possible for a microplate with 384 wells. A level

measurement with optical sensor is not recommended for a microplate with 96 wells.

7.2.3 Filter plates

Abb. 7-5: Filter plate with 96 wells

Fig. 7-5: Filter plate with 96 wells

Filter plates are available for nucleic acid preparation using the vacuum unit.

Each well of a filter plate contains a filter and an opening on the lower side.


English (EN)64

7.2.4 Channeling plate 96

Abb. 7-6: Channeling plate 96

Fig. 7-6: Channeling plate 96

The Channeling plate 96 is used in the vacuum unit. The Channeling plate 96 protects the filter plate

against contamination.

The Channeling plate 96 is positioned on the collection vessel in the vacuum unit. If you insert the

Channeling plate 96, you only can use the 400 mL Reservoir as a collection vessel.

Due to the geometry of filter plates, the Channeling plate 96 is recommended for Qiagen applications

(QIAamp 96 DNA Blood and QIA_Rneasy 96).

7.2.5 PCR plates

Abb. 7-7: PCR plate with 96 wells

Fig. 7-7: PCR plate with 96 wells

PCR plates are available with 96 and 384 wells.

96-well PCR plates are available with a border (skirted), a semi-border (semi-skirted) or without a border

(unskirted).

96-well PCR plates with a semi-border (semi-skirted) can be placed on the thermoblock PCR 96 OC and the

thermoblock PCR 96. 96-well PCR plates without a border (unskirted) can only be placed on the

thermoblock PCR 96.

Tab. 7-1: Placing PCR plates on the worktable

7.2.6 Tube plates

Tube plates are plates with individual vessels. Tube plates are available in various vessel layouts. Tube

plates are treated as normal plates.

Plate with thermoblock with thermoadapter direct

96-well PCR plates skirted x x x

96-well PCR plates semi-skirted x

96-well PCR plates unskirted x


epMotion® 5075

English (EN)

7.3 Reservoirs

Abb. 7-8: Reservoirs 100 mL and 30 mL

Fig. 7-8: Reservoirs 100 mL and 30 mL

Reservoirs make liquid available. Reservoirs are made of polypropylene (PP).

Reservoirs are available in the following sizes:

• 30 mL

• 100 mL

• 400 mL

7.3.1 Reservoir 30 mL

The 30 mL reservoir can be used with all pipette tips.

The 30 mL reservoir has a ribbed base. If the bottom tolerance has been reduced, the information on the

remaining volume is not precise.

The 30 mL reservoir is very narrow at the bottom. If the bottom tolerance is reduced, the reservoir can also

be lifted using pipette tips.

The 30 mL reservoir is particularly well-suited for eight-channel dispensing tools.

The 30 mL reservoir can only be used with a ReservoirRack. The 30 mL reservoir can be tempered in the

ReservoirRack.


The 100 mL reservoir has a ribbed base. If the bottom tolerance has been reduced, the information on the

remaining volume is not precise.

The 100 mL reservoir is particularly well-suited for eight-channel dispensing tools.

The eight-channel dispensing tools TM 50 and TM 300 cannot immerge down to the bottom. An increased

remaining volume remains.

The 100 mL reservoir can only be used with a ReservoirRack. The 100 mL reservoir can be tempered in the

ReservoirRack.


English (EN)66


Abb. 7-9: Reservoir 400 mL

Fig. 7-9: Reservoir 400 mL

The remaining volume of the 400 mL Reservoir is approx. 10 mL.


epMotion® 5075

English (EN)

7.4 epT.I.P.S. Motion

Abb. 7-10: epT.I.P.S. Motion 1 000 μL, 50 μL, 300 μL

Fig. 7-10: epT.I.P.S. Motion 1 000 μL, 50 μL, 300 μL

epT.I.P.S. Motion are special pipette tips for the epMotion.

epT.I.P.S. Motion pipette tips, racks and trays are made of polypropylene (PP). The filter is made of

polyethylene (PE).

Racks and trays are not transported with the gripper.

The optical sensor detects the size of the pipette tips via the Rack coding. The optical sensor also detects if

the pipette tips have a filter. However, the optical sensor does not use the coding to detect if the pipette tips

are in Racks, SafeRacks or in the TipHolder.

NOTICE! Dispensing error due to incorrect handling of pipette tips.Pipette tips warp and change their size during autoclaving.

Do not autoclave pipette tips. Use pipette tips specified as sterile, if necessary.

Do not stack racks that contain pipette tips.

Observe the instructions for use for the epT.I.P.S. Motion Racks and epT.I.P.S. Motion Reloads.


English (EN)68

7.4.1 epT.I.P.S. Motion Racks

Abb. 7-11: epT.I.P.S. Motion Rack

Fig. 7-11: epT.I.P.S. Motion Rack

epT.I.P.S. Motion racks are intended for single use.

epT.I.P.S. Motion racks are specially designed for applications that require sterile work.

Tab. 7-2: epT.I.P.S. Motion racks are available in the following sizes:

Tab. 7-3: epT.I.P.S. Motion racks are available in the following purity grades:

Size of the pipette tips Dispensing tool Color of the rack

50 μL TS 50, TM 50 Light gray

300 μL TS 300, TM 300 Yellow

1000 μL TS 1000, TM 1000 Dark blue

epT.I.P.S. Motion Racks Purity grade

Pipette tips with filter PCR clean

PCR clean and sterile

Pipette tips without filter Eppendorf Quality

Sterile


epMotion® 5075

English (EN)

7.4.2 epT.I.P.S. Motion SafeRacks Abb. 7-12: epT.I.P.S. Motion SafeRack

Fig. 7-12: epT.I.P.S. Motion SafeRack

epT.I.P.S. Motion in SafeRacks are intended for multiple uses. If you use the pipette tips in an application,

use epT.I.P.S. Motion SafeRacks, for example, to absorb buffers in several washing steps.

epT.I.P.S. Motion SafeRacks have a partition to separate pipette tips. During an application, used pipette

tips are returned to the SafeRack. A partition prevents residual liquid from contaminating adjacent pipette

tips. Contamination of samples due to repeated use is prevented because the software permanently

allocates the pipette tips within a specific application to a source position.

In the software, you can set whether pipette tips should be used multiple times (refer to the epMotion

software manual).

Tab. 7-4: epT.I.P.S. Motion SafeRacks are available in the following sizes:

Tab. 7-5: epT.I.P.S. Motion SafeRacks are available in the following purity grades:

NOTICE! Contamination due to the use of the wrong pipette tips.The optical sensor does not detect whether the pipette tips on the worktable are intended for

single use (epT.I.P.S. Motion Rack, epT.I.P.S. Motion Reload) or for multiple use (epT.I.P.S.

Motion SafeRack).

If you use pipette tips several times, equip the entire worktable with epT.I.P.S. Motion

SafeRacks.

Use epT.I.P.S. Motion SafeRacks for a maximum of 6 usage cycles. A usage cycle consists of

aspiration, dispensing and ejecting the tip.

Size of the pipette tips Dispensing tool Color of the rack




epT.I.P.S. Motion SafeRacks Purity grade




English (EN)70

7.4.3 epT.I.P.S. Motion Reloads

epT.I.P.S. Motion Reloads are intended for single use.

The pipette tips are in trays. The trays will be placed in reusable TipHolders, which reduces waste because

no Racks are required.

Tab. 7-6: epT.I.P.S. Motion Reloads are available in the following sizes:

Tab. 7-7: epT.I.P.S. Motion Reloads are available in the following purity grades:

Abb. 7-13: epT.I.P.S. Motion Reloads with packing

Fig. 7-13: epT.I.P.S. Motion Reloads with packing

Abb. 7-14: epT.I.P.S. Motion Reloads and TipHolder

Fig. 7-14: epT.I.P.S. Motion Reloads and TipHolder

Size of the pipette tips Dispensing tool Tray color




epT.I.P.S. Motion Reloads Purity grade


PCR clean and sterile



epMotion® 5075

English (EN)

7.4.4 TipHolder for epT.I.P.S. Motion Reloads

Abb. 7-15: TipHolder

Fig. 7-15: TipHolder

The TipHolder is an adapter that holds the epT.I.P.S. Motion Reloads.

7.4.4.1 epT.I.P.S. Placing Motion Reloads on the TipHolder

Abb. 7-16: Placing the tray on the TipHolder

Fig. 7-16: Placing the tray on the TipHolder

1. Open the Reload packing.

2. Remove the tray with the tips.

3. Place the tray on the TipHolder. The notch in the tray must be on the labeled side of the TipHolder.

1 Tips

2 Tray

3 TipHolder

1

2

3


English (EN)72

73Labware accessories

epMotion® 5075

English (EN)

8 Labware accessories

8.1 Racks

Racks are holders for mounting the same type of individual vessels.

Racks are not transported with the gripper.

8.1.1 Rack for 24 vessels

Abb. 8-1: Rack for 24 vessels

Fig. 8-1: Rack for 24 vessels

Various Racks are available for vessels 12 mm – 17 mm in diameter. Racks are available in 2 heights. The

bores for the vessels are numbered.

The Racks cover the same area as plates. Racks can be positioned at any location.

Racks are coded. The optical sensor uses the code to detect if the Rack is correctly positioned.

8.1.2 Rack for 96 conical vessels

Abb. 8-2: Rack for 96 conical vessels

Fig. 8-2: Rack for 96 conical vessels

The Rack for 96 conical vessels takes up 2 locations on the worktable.

The Rack holds 96 vessels without lid with a content of 1.5 mL or 2 mL. The Rack holds 48 vessels with

fastened lids, e.g. Safe-Lock reaction vessels.

Vessels and accessories may not exceed an overall height of 126 mm. If this overall height is

exceeded, the software generates an error message.

Labware accessoriesepMotion® 5075

English (EN)74

8.1.2.1 Equipping the Rack

If you equip the Rack with vessels with fastened lids, leave each second row of the Rack empty.

Ensure that the lids do not cover the openings of adjacent vessels.

8.1.2.2 Place the Rack on the worktable

1. Place the Rack on the epMotion worktable so that the opening in the lower panel points toward the user.

2. Place the Rack on the positioning pins of the two locations.

8.1.3 0.5/1.5/2.0 mL Rack

Abb. 8-3: 0.5/1.5/2.0 m Rack

Fig. 8-3: 0.5/1.5/2.0 m Rack

The 0.5/1.5/2.0 mL Rack has 24 bores for vessels with a volume of 1.5 mL and 2 mL. If you are using an

adapter, vessels with a volume of 0.5 mL can be inserted.

The 0.5/1.5/2.0 mL Rack has lid holders. The lid holders are on the right, next to the bore for the vessel.

The lid holders vertically clamp the vessel lid.

The 0.5/1.5/2.0 mL Rack is transported with the gripper.


epMotion® 5075

English (EN)

8.1.4 Rack LC

Abb. 8-4: Rack LC 100 μL

Fig. 8-4: Rack LC 100 μL

The Rack LC holds the following capillaries:

• 96 LightCycler capillaries with 20 μL volume

• 96 LightCycler capillaries with 1000 μL volume

The Rack LC has bores for every capillary size. The bores are arranged in an alternating pattern.

The Rack LC is labeled on both of the longer sides. One side has the label Rack LC 20 μL, the other side has

the label Rack LC 100 μL.

8.1.4.1 Equipping the Rack LC

Equip the Rack LC. Only use capillaries of the same size for a single application.

8.1.5 PrepRack

Abb. 8-5: PrepRack

Fig. 8-5: PrepRack

The PrepRack is specially designed for use with the magnetic finger module. You can also use the PrepRack

as the standard rack.

The PrepRack can be placed on the Eppendorf ThermoMixer module and on a thermal module. The

PrepRack is temperature-controlled.

The PrepRack has 24 vessel bores which are numbered row by row. The corresponding lid holder is located

to the right of the bore. The lid holder vertically jams the vessel lid.

The PrepRack is equipped in the same way as the 0.5/1.5/2.0 mL Rack (see p. 77).

The optical sensor detects the PrepRack using its code.

The PrepRack is transported with the gripper.


English (EN)76

8.1.6 ReagentRack

Abb. 8-6: ReagentRack

Fig. 8-6: ReagentRack

The ReagentRack holds a tray with reagents from the Eppendorf MagSep kits.

The ReagentRack cannot be placed on the Eppendorf ThermoMixer module.

The optical sensor detects the tray with reagents using its code.

The ReagentRack is not transported with the gripper.

8.1.6.1 Placing the ReagentRack on the worktable

The ReagentRack cannot be placed at location B0.

Place the ReagentRack on the worktable so that the ReagentRack tabs are positioned between the

positioning pins for the location.

Remove lids from all vessels in the ReagentRack.


epMotion® 5075

English (EN)

8.1.7 0.5/1.5/2.0 mL thermorack

Abb. 8-7: 0.5/1.5/2.0 mL thermorack

Fig. 8-7: 0.5/1.5/2.0 mL thermorack

The 0.5/1.5/2.0 mL thermorack has 24 bores for vessels with a volume of 1.5 mL and 2 mL. If you are using

an adapter, vessels with a volume of 0.5 mL can be inserted.

The 0.5/1.5/2.0 mL thermorack has lid holders. The lid holders are on the right, next to the bore for the

vessel. The lid holders vertically clamp the vessel lid.

0.5/1.5/2.0 mL thermorack is transported with the gripper.

0.5/1.5/2.0 mL thermorack is temperature-controlled and designed for use on thermal modules.

Thermoracks can be cooled in the lab refrigerator.

Tab. 8-1: Guide values for tempering thermoracks

Thermorack Equipping Filling volume per vessel

Time taken to heat from 0°C to 10°C

0.5/1.5/2.0 mL thermorack Safe-Lock tube 1.5 mL 1000 μL ~ 30 min


English (EN)78

8.1.8 Thermorack TMX

Abb. 8-8: Thermorack TMX

Fig. 8-8: Thermorack TMX

The thermorack TMX is designed for use on the Eppendorf ThermoMixer module. The thermorack TMX is

lighter than a normal thermorack and provides for higher speeds during mixing. The thermorack TMX has

a lower heat capacity but quick heat transfer. The thermorack TMX quickly reaches the target temperature.

The thermorack TMX does not keep the target temperature without tempering.

The thermorack can be cooled in the lab refrigerator.

The thermorack TMX is available for vessels with a volume of 0.5 mL. If you are using an adapter, vessels

with a volume of 0.5 mL can be inserted.

8.1.9 Thermorack rotor/tubes

Abb. 8-9: Thermorack rotor/tubes

Fig. 8-9: Thermorack rotor/tubes

The thermorack rotor/tubes can be tempered on a thermal module in the epMotion. The thermorack rotor/

tubes reduces the time until the samples reach the target temperature.

The epMotion can fill the tubes on the thermorack rotor/tubes with sample material and extract sample

material from the tubes. The epMotion can fill the positions on the Qiagen Rotor Disc with sample material.

The epMotion cannot extract sample material from the positions on the Qiagen Rotor Disc.


epMotion® 5075

English (EN)

The following labware can be inserted into the thermorack rotor/tubes:

• a Qiagen Rotor-Disc 72 or a Qiagen Rotor-Disc 100

• in addition a maximum of 20 Eppendorf micro test tubes 1.5 mL/2.0 mL or a maximum of 20 Sarstedt

screw cap tubes 1.5 mL

The thermorack can be cooled in the lab refrigerator.

There is a ring in the delivery packing. The ring fixes the Qiagen Rotor-Disc on the thermorack rotor/tubes.

A lid and an adapter for putting the lid on are available for the thermorack rotor/tubes.

8.1.9.1 Placing the thermorack on the worktable

The thermorack rotor/tubes occupies 2 places on the worktable. The place directly behind the thermorack

cannot be equipped with labware.

During application, the lid of the thermorack rotor/tubes can be removed by means of the gripper and

transported. The gripper places the lid on an adapter. The adapter must be in position B0.

1. Place the equipped thermorack rotor/tubes on a place in the following row:

• Row C

2. Place the ring onto the Qiagen Rotor Disc.

3. Place the adapter for the lid of the thermorack rotor/tubes on position B0.


English (EN)80

8.1.10 ReservoirRack

Abb. 8-10: Equipped ReservoirRack

Fig. 8-10: Equipped ReservoirRack

The ReservoirRack is used for holding reservoirs and ReservoirRack modules. The ReservoirRack columns

are numbered.

The ReservoirRack can hold a maximum of 7 reservoirs or 7 different ReservoirRack modules. Reservoir

Racks can be equipped in any order.

The ReservoirRack cannot be placed on the Eppendorf ThermoMixer module.

8.1.10.1 Equipping ReservoirRacks

Position 1 and position 7 are not tempered as well as the other positions in the ReservoirRack.

Place the ReservoirRack modules into the ReservoirRack with the code facing the rear.

Insert the reservoirs into the ReservoirRack with the code facing any direction.

8.1.10.2 Placing the ReservoirRack on the worktable

The ReservoirRack cannot be placed at A locations or location B0.

Place the ReservoirRack on the worktable so the ReservoirRack tabs are positioned between the


1 Reservoir

2 ReservoirRack module

3 ReservoirRack columns

4 ReservoirRack

4

1 2

3


epMotion® 5075

English (EN)

8.1.11 ReservoirRack 3

Abb. 8-11: ReservoirRack 3

Fig. 8-11: ReservoirRack 3

The ReservoirRack 3 with inserted reservoirs can be used as a waste container for liquid waste.

The ReservoirRack 3 can be equipped with a maximum of 3 reservoirs for 30 mL or 100 mL. The

ReservoirRack 3 cannot be equipped with ReservoirRack modules.

8.1.11.1 Placing the ReservoirRack 3 on the worktable

You only can place the ReservoirRack 3 on location A5 of the epMotion 5075m and epMotion 5075t.

Place the ReservoirRack 3 on the worktable so that the ReservoirRack is positioned between the



English (EN)82

8.1.12 ReservoirRack modulesAbb. 8-12: ReservoirRack modules

Fig. 8-12: ReservoirRack modules

A ReservoirRack module is equipped with vessels or a reservoir. All of the vessels inserted into a

ReservoirRack module must be of the same type. The ReservoirRack module is inserted into a

ReservoirRack.

The ReservoirRack modules TC are suitable for temperature control.

Tab. 8-2: Temperature control duration of tubes in the corresponding ReservoirRack modules

1 ReservoirRack module TC for 2 reaction vessels Ø 29 mm


3 ReservoirRack module TCfor 1 reservoir 100 mL

4 ReservoirRack module TC for 1 reservoir 30 mL

5 ReservoirRack module TCfor 4 reaction vessels Ø 16 mm


7 ReservoirRack module TCfor 4 Safe-Lock tubes 1.5 mL/2 mL with adapter

for 0.5mL

8 ReservoirRack module TC for 8 PCR tubes 0.2 mL

9 ReservoirRack module TC for 4 Eppendorf Tubes 5 mL

Micro test tube Temperature control from 23°C to 4°C Temperature control from 23°C to 37°C

Set temperature Temperature control duration

Set temperature Temperature control duration

PCR tube 0.2 mL 3°C Approx. 15 min 38°C Approx. 8 min

Safe-Lock tube 0.5 mL 3°C Approx. 20 min 38°C Approx. 12 min



Tube Ø 12 mm 3°C Approx. 30 min 38°C Approx. 17 min

Tube Ø 16 mm 3°C Approx. 30 min 38°C Approx. 17 min

Tube 5 mL 3°C Approx. 30 min 38°C Approx. 17 min

Conical tube 15 mL 2°C Approx. 30 min 38°C Approx. 17 min

Conical tube 50 mL 3°C Approx. 39 min 39°C Approx. 23 min

Reservoir 30 mL 1°C Approx. 21 min 39°C Approx. 15 min

Reservoir 100 mL 1°C Approx. 46 min 40°C Approx. 28 min


epMotion® 5075

English (EN)

The table shows the time it takes to reach the set temperature if the user has entered the set temperature in

the software.

8.1.12.1 Connecting the ReservoirRack module for reservoirs to an adjacent module

The ReservoirRack modules for the 30 mL and 100 mL reservoirs cannot be placed into the ReservoirRack

separately. The ReservoirRack modules must be connected to the adjacent ReservoirRack.

Abb. 8-13: Connecting the 30 mL TC ReservoirRack module to an adjacent module.

Fig. 8-13: Connecting the 30 mL TC ReservoirRack module to an adjacent module.

There are 2 connecting bars on one side of the ReservoirRack modules for the 30 mL and 100 mL

reservoirs.

Insert the 2 connecting bars into the adjacent ReservoirRack modules.

8.1.12.2 Adjusting the height of the vessels

Abb. 8-14: Setting the ReservoirRack module to vessel height

Fig. 8-14: Setting the ReservoirRack module to vessel height

1 Reservoir 30 mL

2 ReservoirRack module TC for 30 mL Reservoir

3 Connecting bar

4 Adjacent module

1 Positioning screwin resting position

2 Positioning screwat height of 50 mm

3 ReservoirRack module

1

2

3

4

2

1

3


English (EN)84

You can position tubes at heights of 50 mm, 60 mm, 70 mm, 80 mm and 90 mm in each ReservoirRack

module. At these heights there is one bore on the front and one on the rear of the ReservoirRack module.

Screw the 2 positioning screws into the bores at the required height.

8.2 Adapter

The following adapters are available for the epMotion:• Height adapter

• Thermoadapter

• Disc Adapter

8.2.1 Height adapter

Abb. 8-15: Height adapter

Fig. 8-15: Height adapter

The height adapters are used to offset the various heights of the labware. The tool holder does not need to

offset the height differences of the labware and remains at one height. The shorter paths of this tool holder

allow the application to run more quickly.

The height of the adapter is specified on the adapter. The combined height of the labware and the adapter

may not exceed 126 mm.

Height adapters are not transported with the gripper.

Tab. 8-3: Available height adapters

Adapter Use with

40 mm • epT.I.P.S. Motion racks 50 μL and 300 μL in size

55 mm • DWP plates

• 400 mL Reservoirs

• Thermoblock with PCR plates

85 mm • Almost all MTP plates with 6 – 384 wells

• Almost all PCR plates with 96 and 384 wells

• PCR thermoblock with PCR plates


epMotion® 5075

English (EN)

8.2.2 Vac Holder vacuum holder

Abb. 8-16: Vac Holder vacuum holder

Fig. 8-16: Vac Holder vacuum holder

The vacuum holder is an adapter. The vacuum holder is used to place the vacuum frame on the worktable.

The vacuum holder is placed on location C4 of the worktable.

8.2.3 Thermoadapter

Abb. 8-17: Thermoadapter DWP 96

Fig. 8-17: Thermoadapter DWP 96

A thermoadapter is an adapter that can be temperature-controlled. Thermoadapters are available for

deepwell plates and PCR plates.

A thermoadapter can be placed on the worktable with or without plates.

A thermoadapter can be tempered on the thermal module.

A thermoadapter is not transported using the gripper. The gripper can remove a plate from the

thermoadapter or place it on the thermoadapter.

Tab. 8-4: Guide values for tempering thermoadapters

Thermoadapter Equipping Filling volume per vessel

Time taken to heat from 0°C to 10°C

Thermoadapter PCR 96 96-well twin.tec PCR plate 150 μL ~ 14 min

Thermoadapter PCR 384 384-well twin.tec PCR plate 25 μL ~ 10 min


English (EN)86

8.2.4 Thermoadapter LC Samples

Abb. 8-18: Thermoadapter LC Samples

Fig. 8-18: Thermoadapter LC Samples

The LC Samples thermoadapter is a tube holder for automatically filling MagNA Pure LC Sample

Cartridges. Thermoadapters and cartridges are a fixed combination for the epMotion.

The Thermoadapter LC Samples can be tempered up to 70°C.

8.2.5 Thermoadapter Microplate 96/V/U

Abb. 8-19: Thermoadapter Microplate 96/V/U

Fig. 8-19: Thermoadapter Microplate 96/V/U

The Thermoadapter Microplate 96/V/U is not firmly attached to a plate.

The following labware can be inserted into the Thermoadapter Microplate 96/V/U:

• Microplate 96/V

• Microplate 96/U

The Thermoadapter Microplate 96/V/U can be tempered on the Eppendorf ThermoMixer module and the

thermal module.

The Thermoadapter Microplate 96/V/U is not transported by means of the gripper. Microplates can be

placed in the adapter by means of the gripper. Microplates can be taken out of the adapter by means of the

gripper.


epMotion® 5075

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8.2.6 Thermoadapter Frosty

Abb. 8-20: Thermoadapter Frosty

Fig. 8-20: Thermoadapter Frosty

The Thermoadapter Frosty cools samples.

The Thermoadapter Frosty is a modified height adapter. which includes a PCR-Cooler. A PCR plate is

placed on the Thermoadapter Frosty. The PCR-Cooler cools the samples in this plate. The Thermoadapter

Frosty can only be used with skirted PCR plates.

8.2.6.1 Preparing the Thermoadapter Frosty

1. Cool the PCR-Cooler. by placing the PCR-Cooler upside down in the lab refrigerator or in the upright

freezer at -20°C.

2. Insert the PCR-Cooler in the Thermoadapter Frosty.

3. Place a PCR plate on the Thermoadapter Frosty.

The PCR-Cooler changes color if the temperature exceeds 7°C. The color changes from purple

to pink or from dark blue to light blue. The color in the recesses of the PCR-Cooler is a

decisive factor for the temperature of the samples.


English (EN)88

8.3 Thermoblock

A thermoblock is an adapter that can be temperature-controlled.

There are 2 types of thermoblocks:

• Thermoblocks: Thermoblock in which a plate is inserted.

• Thermoblocks with plates: Thermoblock that is firmly attached to a plate.

A thermoblock can be tempered on a thermal module.

Store the thermoblock in the laboratory refrigerator to cool it.

A thermoblock is transported by the gripper.

8.3.1 Thermoblock PCR 96

Abb. 8-21: Thermoblock PCR 96

Fig. 8-21: Thermoblock PCR 96

In the Thermoblock PCR 96, thermoblock and plate are firmly connected to each other.

8.3.1.1 Thermoblock PCR 96 equipped with 0.2 mL vessels

Abb. 8-22: Tube lid rotated 45° to the surface of the thermoblock

Fig. 8-22: Tube lid rotated 45° to the surface of the thermoblock

Position the tubes in columns. Leave every other column vacant for the tube lids.

A maximum of 48 tubes can be positioned with the lid in the thermoblock.

Thermoblock Plate firmly connected to the thermoblock

Filling volume per vessel

Time taken to heat from 0 °C to 10 °C

PCR 96 96-well twin.tec PCR plate 150 μL ~ 14 min


epMotion® 5075

English (EN)

8.3.2 Thermoblock PCR 384

Abb. 8-23: Thermoblock PCR 384

Fig. 8-23: Thermoblock PCR 384

In the thermoblock PCR 384, thermoblock and plate are firmly connected to each other.

8.3.3 Thermoblock PCR 96 OC

Abb. 8-24: Thermoblock PCR 96 OC

Fig. 8-24: Thermoblock PCR 96 OC

The Thermoblock PCR 96 OC (OC = Orientation Control) has been designed to safely open sealed plates.

Safe opening is achieved by piercing the seal with a pipette tip. You can find information on the

configurations for opening sealed plates in the software operating manual.

The Thermoblock PCR 96 OC is not firmly attached to a plate. In the Thermoblock PCR 96 OC, semi-skirted

plates with a right beveled corner can be used.

The Thermoblock PCR 96 OC facilitates the work with sealed plates whose labels are illegible. For this

purpose the Thermoblock PCR 96 OC has a pin at the right corner. Plates are inserted so that their beveled

corner is also at the lower right. This ensures that position A1 of the plate is at the top left.

The Thermoblock PCR 96 OC is suitable for high speeds. The maximum speed is 1700 rpm.

Thermoblock Plate firmly connected to the thermoblock

Filling volume per vessel

Time taken to heat from 0 °C to 10 °C

PCR 384 384-well twin.tec PCR plate 25 μL ~ 10 min

Labware Recommended maximum speed in rpm

Maximum quantity of sample material

Thermoblock PCR 96 OC 1600 With a non-sealed plate < 140 μL


English (EN)90

8.3.3.1 Positioning of a plate on the Thermoblock PCR 96 OC

Prerequisites

• Semi-skirted plate with beveled corner

1. Check whether the clamps on the Thermoblock PCR 96 OC are bent.

2. Replace the clamps if they are bent. Use spare clamps.

3. Place a semi-skirted plate on the thermoblock. Insert the plate so that the beveled corner is at the lower

right.

4. Check that the plate is correctly positioned in the thermoblock.

The plate is held firmly in place by the clamps.

The plate sits firmly in the bores of the thermoblock.

8.3.4 Thermoblock and thermoadapter comparison

Abb. 8-25: Thermoblock and thermoadapter comparison

Fig. 8-25: Thermoblock and thermoadapter comparison

Thermoblocks and thermoadapters are available for some plates, e.g. for PCR plates with 384 wells.

Thermoblocks and thermoadapters are labeled on the side. The label includes the name of the

manufacturer, the type of adapter and the name of the appropriate labware.

Thermoadapters and thermoblocks also differ in their bar length. Thermoadapters have shorter bars than

thermoblocks.

PCRPCRThermoadapterThermoadapter384384PCRPCRThermoblockThermoblock 384384


epMotion® 5075

English (EN)

8.4 Waste container8.4.1 Waste box

Abb. 8-26: Waste box

Fig. 8-26: Waste box

The waste box can hold a maximum of 300 pipette tips of 1000 μL in size.

8.4.1.1 Placing the disposal bag in the waste box

Use a disposal bag to protect the waste container from contamination.

Prerequisites

• Disposal bag

• Clamping ring

1. Place the disposal bag in the waste box.

2. Fasten the disposal bag with the clamping ring.

3. Tightly pull the edge of the disposal bag downward.


English (EN)92

8.4.2 LiquidWasteTub with lid

Abb. 8-27: LiquidWasteTub with lid

Fig. 8-27: LiquidWasteTub with lid

The LiquidWasteTub is a container for liquid waste and is hung in the waste container. The LiquidWasteTub

has a working volume of 115 mL.

The optical sensor detects if the LiquidWasteTub is present. The optical sensor also detects the filling level

of the LiquidWasteTub.

The lid of the LiquidWasteTub prevents contamination due to splashes from the liquid waste. The

LiquidWasteTub only can be used with single-channel dispensing tools.

The LiquidWasteTub is reusable. You can decontaminate and clean the LiquidWasteTub (see

Decontamination and cleaning on p. 107).

You have to define in the software that the waste container is equipped with the LiquidWasteTub.

WARNING! Danger due to contamination of the LiquidWasteTub.The outer surface of the LiquidWasteTub may become contaminated by pipette tips in the

waste container.


Decontaminate the outer surface of the LiquidWasteTub.

1 Opening for dispensing tool

2 Opening for level measurement

3 Lid

4 LiquidWasteTub

123

4


epMotion® 5075

English (EN)

8.4.2.1 Using the LiquidWasteTub

1. If required, insert the disposal bag into the waste container.

2. Place LiquidWasteTub on the edge of the waste container.

The tab of the LiquidWasteTub must protrude over the clamping ring of the waste container.

3. Put the lid on the LiquidWasteTub.

4. Place waste container on the worktable.

The LiquidWasteTub must be located on the right-hand side of the waste container.

NOTICE! Risk of collisions due to loose fitting LiquidWasteTub.If the LiquidWasteTub is not properly seated in the waste container, the dispensing tool may

collide with the LiquidWasteTub when the tips are ejected.

Always use the waste container with the clamping ring.


English (EN)94

95Operation

epMotion® 5075

English (EN)

9 Operation9.1 First steps

This chapter describes how to operate the epMotion and carry out the applications.

Information on how to create applications is available in the software manual.

9.1.1 Switch on the epMotion.

Prerequisites

• The epMotion has been installed and commissioned by service technicians authorized by Eppendorf.


2. Switch on the epMotion at the mains/power switch.

The carrier travels to the home position.

3. Switch on the MultiCon PC and start epBlue.

9.1.2 Using the touch screen

Touch the touch screen with a finger.

When operating the touch screen, you can also wear thin rubber gloves.

Or you can use a stylus which is suitable for capacitive touch screens.

9.1.3 Using the mouse

You can use a mouse.

Connect the USB mouse to one of the USB ports of the control device.

The mouse arrow appears on the screen as soon as the mouse has been recognized.

9.1.4 Using the keyboard

You can use a keyboard.

Connect the USB keyboard to one of the USB ports of the control device.

OperationepMotion® 5075

English (EN)96

9.1.5 Backing up data

In order to transfer or back up data, you need a USB storage medium.

1. Connect the USB storage medium to one of the USB ports.

The storage medium is ready for operation after 5 seconds.

2. Execute the required function in the software (refer to the epMotion software manual).

9.1.6 Working with epMotion

The epMotion workflow consists of the following steps.

1. Create an application (refer to the epMotion software manual).

2. Equip the worktable (see p. 96).

3. Execute the application (see p. 101).

4. Complete your work (see p. 104).

Step 1 is described in the software manual. Steps 2 – 4 are described in the following sections.

9.2 Placing the tools and labware on the worktable

In order to prepare the epMotion for an application, perform the following steps:

• Displaying the epBlue worktable in the software

• Positioning tools

• epT.I.P.S. Motion

• Placing the waste container

• Positioning labware

NOTICE! Data loss and malfunction due to malicious software.Malicious software (e.g., a computer virus) on the USB storage medium can be transferred to

the computer.

Before connecting the USB flash drive, check the USB flash drive using current antivirus

software.

NOTICE! Data loss due to missing backup copy or incorrect storage of data carriers.User accounts, applications, labware and logs are stored in a database. If this database gets

damaged (e.g. due to a hardware defect), the information is lost.

Eppendorf is not liable for data loss of data. Eppendorf is not liable for damage arising as a

result of data loss.

Backup the database on a regular basis. Use the Backup function of the epBlue software.

Save stored data on external data carriers.

Store the storage media according to the manufacturer's specifications.

NOTICE! Malfunction due to third-party software.Third-party software can impair the functionality of the epBlue software.

Only install software released by Eppendorf.

97Operation

epMotion® 5075

English (EN)

9.2.1 Display epBlue worktable

To display the epBlue worktable, proceed as follows:

1. Open the application.

2. Select the Run area or step (refer to the epMotion software manual).

The epBlue worktable shows graphically how tools and labware are placed on the epMotion worktable

3. Place the tools and labware on the epMotion worktable as shown.

Details on placement can be found in the following chapters.

NOTICE! Tool collisions with incorrectly positioned labware.Labware must be positioned on the epMotion worktable as defined in the application.

Tools and labware are damaged if there is a collision. A collision can lead to sample loss.

Check the position of the labware before starting the application.

epMotion 5075

5075 v, vt

epMotion 5075

5075 t, vt, m max. 2× TM1, TM2

epMotion 5075

5075 l, v max. 3× TM1, TM2, TM3

epMotion 5075

5075 t, vt, m

TM 1 TM 2 TM 3Odpady

A2T0 T1

T4T2

T3 A3 A4 A5

B3 B4 B5B2B1B0

VAC

epT.I.P.S.Adapter

epMotion 5075

5075 m PrepRack

ReservoirRack

epMotion 5075

5075 v, vt Vac HolderepT.I.P.S.

Rack 3

epMotion 5075

5075 t, m

C1 C2 C3 C4 C5

TMX

Vac Lid

300mL/400mL

Liquid Waste Tub

5050

MagSep


English (EN)98

9.2.2 Placing dispensing tools on the worktable

You can place 4 dispensing tools on the worktable.

When the application is started, epMotion checks if the required tools are available. The order of the

dispensing tools can be user-defined.

Place the dispensing tool with the gold contact to the right rear into the holders T1 to T4.

9.2.3 Placing the gripper on the worktable

The gripper is required for applications in which labware is transported.

Mounting the gripper on the holderAbb. 9-1: Open (1) and close (2) the gripper arms.

Fig. 9-1: Open (1) and close (2) the gripper arms.






1 Gripper arms

2 Pin for holding the gripper arms

3 Holder

4 Mandrels

21

1234

99Operation

epMotion® 5075

English (EN)

1. Place the holder so that the pin is on the right side.

2. Set the gripper down.

The gripper mandrels must engage in the bores of the holder.

The pin must engage in the finger hole on the inside of the right gripper arm.

Placing the gripper on the worktable

3. Place the gripper and holder at the T0 location.

4. Position the gripper and holder with the gripper's gold contacts towards the rear of the epMotion.

9.2.4 epT.I.P.S. Placing Motion on the worktable

The optical sensor detects the type of pipette tips using the coding on the tray. The epMotion removes the

pipette tips from the rack column by column. Additional information is available on the epT.I.P.S (see p. 67)

1. When using epT.I.P.S. Motion Reloads, place the tray on the TipHolder.

2. Place the rack or TipHolder on the worktable location with the label pointing toward the front.

3. Remove the lid.

Observe the instructions for use for the epT.I.P.S. Motion Racks and epT.I.P.S. Motion Reloads.

NOTICE! Dispensing error due to incorrect handling of pipette tips.Pipette tips warp and change their size during autoclaving.

Do not autoclave pipette tips. Use pipette tips specified as sterile, if necessary.

Do not stack racks that contain pipette tips.

NOTICE! Incorrect stock detection due to missing pipette tips.The optical sensor checks the start position and end position of the pipette tips in the rack.

The optical sensor checks the start position and end position of the pipette tips in the rack.

Do not remove any pipette tips from the rack.

NOTICE! Contamination due to the use of the wrong pipette tips.The optical sensor does not detect whether the pipette tips on the worktable are intended for

single use (epT.I.P.S. Motion Rack, epT.I.P.S. Motion Reload) or for multiple use (epT.I.P.S.

Motion SafeRack).

If you use pipette tips several times, equip the entire worktable with epT.I.P.S. Motion

SafeRacks.


English (EN)100

9.2.5 Placing the waste container on the worktable

1. Insert the disposal bag into the waste container.

2. If necessary, insert the LiquidWasteTub into the waste container (see p. 93).

3. Insert the waste container into the epMotion.

9.2.6 Placing the labware on the worktable

Particularities on the placement can be found in the chapter on labware (see Labware accessories on p. 73).

9.2.6.1 Tubes

1. Open the tubes.

2. Put the tubes upright into the racks.

Put tubes with lid only into Racks with lid holder.

3. Lock any fastened lids at the lid holders on the Rack.

9.2.6.2 ReservoirRack modules

Insert the ReservoirRack modules in the ReservoirRack (see p. 80).

9.2.6.3 Plates, racks and adapters

The labware may not have any play at the epMotion worktable location.

1. Align the labware.

• Align the racks and adapters on the epMotion worktable in such a way that the writing faces the user.

• Align the plates on the epMotion worktable in such a way that position A1 of the plate is located at

the rear on the left.

NOTICE! Collisions or dispensing errors due to uneven or canted labware.Labware must lie flat on the worktable.

Do not tilt the labware.

NOTICE! Collision with tube lids.When lids block the tube opening, this can cause a collision with the dispensing tool.

Position the tube lid so that the tube opening is not covered.

101Operation

epMotion® 5075

English (EN)

Plates, racks, epT.I.P.S Motion Racks, and thermoadapter

3. If required, tighten the retention clip by hand.

The labware must fit closely the positioning pins of the location.

Height adapter, TipHolder and ReservoirRack

9.3 Executing an application

9.3.1 Carry out a test run

If you are executing an application for the first time, carry out a test run.

Check the application as follows:

1. Fill labware with defined volumes. Use demineralized water or a test liquid for the test run.

2. Place labware and tools on the epMotion worktable.

3. Activate the optical sensor.

2. Position the labware so the spring clip holder

pushes the labware against the positioning pins.

4. Put the height adapter, TipHolder and

ReservoirRack on the positioning pins.

Details on starting and controlling applications can be found in the software operating

manual.

Carry out the test run with a liquid having similar physical properties as the liquid to be used

in the application.


English (EN)102

4. Fully execute the application.

5. Check to see if the application was executed without errors.

• The dispensing is precise and correct.

• No liquid sprays out of the reaction vessels. Contamination is unlikely.

Information on optimizing the dispensing can be found in the software operating manual.

9.3.2 Starting the application

Prerequisites

• Application is selected.

• Labware and tools are placed on the epMotion worktable.(see p. 96)

1. Start the application.

2. Select the optical sensor settings for this run.

A detailed description of the optical sensor can be found in the software operating manual.

3. For applications with a variable number of samples, enter the number of samples.

4. If the level measurement is deactivated, enter the filling volume of the vessels using the unit μL.

• The epMotion checks if the required tools are available.

• If necessary, the optical sensor checks the labware and levels.

• The application is executed. The software displays the status of the application.

NOTICE! Faulty level measurement due to air bubbles.If there are air bubbles in the vessels, the optical sensor measures the level incorrectly.

Check whether there are air bubbles in the vessels before starting the application.

To remove air bubbles, carefully tap the labware on the work surface.

The optical sensor has the following functions:

Measure filling level in vessels• The optical sensor determines the filling level in the position of the labware. There are 2 ways of

doing this:

– The optical sensor determines the filling level in all positions used.

– The optical sensor determines the filling level in the first position, in the last position and in 8

randomly determined positions.

– The optical sensor cannot measure the filling level in the following labware: 384-well plates,

Qiagen rotor disc 72, Qiagen rotor disc 100.

Detect pipette tips• The optical sensor determines the quantity and the positions of the ep.T.I.P.S. pipette tips. Motion

pipette tips in TipHolders and Racks by Eppendorf.

Check labware on the worktable• The optical sensor scans the code on the labware.

• epBlue checks if the labware on the epMotion worktable matches the labware on the epBlue

worktable.

103Operation

epMotion® 5075

English (EN)

9.3.3 Controlling the application

9.3.3.1 Stopping the application immediately

You can stop the application immediately, e.g. in case of risk of collision. Proceed as follows:

Open the front hood.

• The application will be stopped immediately.

• The Eppendorf ThermoMixer module is not stopped.

You can continue or abort the application.

9.3.3.2 Interrupting the application

Stop the application with the button (refer to the epMotion software manual).

The current movement is ended. Then the application stops.

You can continue or abort the application.

9.3.3.3 Continuing the application

Prerequisites

• Labware and tools on the epMotion worktable were not changed.

• The levels of the vessels have not been changed.

• The carrier has not been moved manually.


2. Continue the application with the button (refer to the epMotion software manual).

CAUTION! Burns from hot thermal module.The thermal module heats up during operation. The software displays the current temperature

of the thermal module.

Do not touch the hot thermal module.

Wait until the thermal module has cooled down.

CAUTION! Risk of injury due to the movement of the carrier.The carrier may still be moving when front hood of the epMotion is opened.

Wait until the carrier is standing still before reaching into the device.


English (EN)104

9.3.3.4 Aborting the application

An application cannot be continued after it has been aborted.

Prerequisites

• The application was stopped or interrupted.

Abort the application with the button (refer to the epMotion software manual).

• The application will be aborted.

• The tool holder returns the tool to its original location.

• The carrier travels to the home position.


Exit Application Runner.

You can start a new application.

9.4 Completing your work9.4.1 Cleaning up the worktable

Complete the following steps after the end of the application.

1. If necessary, switch off the HEPA filter.

2. Seal the vessels.

3. Remove the labware from the worktable.

4. Empty the waste container.

5. If necessary, decontaminate the device and the labware (see Decontamination and cleaning on p. 107).

6. Use a paper towel to soak up the condensation water on the thermal module or Eppendorf ThermoMixer

module.

NOTICE! Contamination due to multiple use of pipette tips.If you abort an application with multiple use of pipette tips, epT.I.P.S. Motion SafeRacks

contain used pipette tips. The device does not detect which pipette tips have already been

used.

Dispose of epT.I.P.S. Motion SafeRacks directly after aborting the application.






waste container.



105Operation

epMotion® 5075

English (EN)

9.4.2 Switch off epMotion

Perform the following steps:

1. Exit epBlue (refer to the epMotion software manual).

2. Move the MultiCon PC down and switch off.

3. Switch off the epMotion using the mains/power switch.

NOTICE! Data loss due to incorrect power-off of the device.If you switch off the device without ending the running processes, data will be lost.

Switch off the device as described in the operating manual.


English (EN)106

107Maintenance

epMotion® 5075

English (EN)

10 Maintenance10.1 Decontamination and cleaning

10.1.1 Cleaning

Auxiliary equipment

• Deionized water

• Lint-free cloths

• Cotton swabs

Prerequisites

• The device is switched off and disconnected from the mains/power line.

1. Moisten the lint-free cloth and cotton swab with deionized water.

2. Clean the surfaces with the lint-free cloth. Clean hard to access surfaces with cotton swabs.

3. Allow the surfaces to dry.






waste container.






NOTICE! Material damage caused by corrosive cleaning agents.Cleaning and decontamination agents can contain corrosive substances. Metal surfaces on the

device become damaged by corrosion.

Use the cleaning agents and disinfectants specified in the operating manual.

MaintenanceepMotion® 5075

English (EN)108

10.1.2 Decontamination methods and decontamination agents

Tab. 10-1: Decontamination methods and decontamination agents

Decontamination method Decontamination agent For use on

Wipe decontamination 70% (v/v) ethanol Worktable

Waste container

LiquidWasteTub

Housing

Worktable base adapter

MultiCon PC

EasyCon Tablet

Barcode reader

Vacuum unit

Lid, thermo lid and sealing mat of the

vacuum unit

Labware

Dispensing tools

3%–4% sodium hypochlorite

solution

Waste container

LiquidWasteTub

Dispensing tools

Gripper

Vacuum unit


vacuum unit

Elution plate adapter

Racks

Thermoblocks

Thermoadapter

Height adapter

TipHolder

Spray decontamination

outside the device

70% (v/v) ethanol Waste container

LiquidWasteTub

Racks

Thermoblocks

Thermoadapter

Height adapter

TipHolder

UV irradiation UV lamp of the CleanCap

Irradiation period of 15 min

Worktable

Eppendorf ThermoMixer module

Thermal modules

109Maintenance

epMotion® 5075

English (EN)

10.1.3 Carrying out wipe decontamination

Use the wipe decontamination to clean device, tools, accessories and labware. Details can be found in the

(see p. 108) chapter.

Auxiliary equipment

• Decontamination agent

• Deionized water

• Lint-free cloths

• Cotton swabs

Prerequisites


1. Moisten the lint-free cloth and cotton swab with decontamination agent.

2. Clean the surfaces with the lint-free cloth. Clean hard to access surfaces with cotton swabs.

The surfaces are moistened with decontamination agent.

3. Allow the decontamination agent to take effect.

4. Wipe the decontamination agent with deionized water.

5. Allow the surfaces to dry.

Autoclaving 121°C

100 kPa overpressure

20 min

Waste container

LiquidWasteTub

Dispensing tools

Gripper

Racks

Thermoblocks

Thermoadapter

Height adapter

TipHolder


vacuum unit

Elution plate adapter

Decontamination method Decontamination agent For use on


English (EN)110

Wipe decontamination on the dispensing tool

10.1.4 Carrying out the spray decontamination

Auxiliary equipment

• Decontamination agent in spray bottle

• Deionized water

1. Remove the labware and the tools from the device.

2. Spray decontamination agents on the labware and tools.

3. Allow the decontamination agent to take effect.

4. Wipe the decontamination agent with deionized water.

5. Let the labware and tools dry.

Abb. 10-1: Removing the ejector sleeve of the single-channel dispensing tool

Fig. 10-1: Removing the ejector sleeve of the

single-channel dispensing tool

6. Remove the ejector sleeve of the single-channel

dispensing tool.

7. Carry out wipe decontamination as described.

NOTICE! Damage to device from the ingress of liquid during spray decontamination.

Do not clean the device by means of spray decontamination.

Remove the labware and tools from the device before cleaning the labware and tools using

spray decontamination.

111Maintenance

epMotion® 5075

English (EN)

10.1.5 Carrying out the UV irradiation

You may only carry out UV irradiation for an epMotion with CleanCap.

Prepare the device and carry out the UV irradiation as described (see UV lamp on p. 47).

10.1.6 Autoclaving

Prerequisites

• 121°C at 100 kPa overpressure are set on the autoclave.

• Any chemical decontamination agents have been removed.

1. Autoclave the tools and labware for at least 20 min.

2. Rinse the tools and labware with deionized water.

3. Let the tools and labware dry.

10.2 Decontamination before shipment

NOTICE! Damage on the tool due to autoclaving.Tool is damaged by excessive temperature in the autoclave.

Make sure that the temperature in the autoclave is 121 °C – 126 °C.

Make sure that the tools do not touch the wall of the autoclave.

WARNING! Risk to health from contaminated device

1. Follow the instructions in the decontamination certificate. You can find it as a PDF file on

our website (www.eppendorf.com/decontamination).

2. Decontaminate all the parts you would like to dispatch.

3. Include the fully completed decontamination certificate in the packing.


English (EN)112

10.3 Servicing the device

Have the UV lamp replaced by an authorized service partner once every 2 years.

Have the HEPA filter replaced by the authorized service partner once per year.

Have each dispensing tool serviced by the authorized service partner after 100,000 full strokes or

200,000 strokes.

Have the sealing of the vacuum unit replaced by the authorized service partner after 300 applications.

10.4 Servicing the vacuum unit10.4.1 Replacing the seals of the vacuum unit

10.4.2 Replacing the silicone mat of the Vac Lid

1. To remove the old silicone mat, undo the screws on the bottom of the Vac Lid.

2. Remove the silicone mat.

3. Place a new silicone mat on the bottom of the Vac Lid.

4. Insert and tighten the screws.

The screw heads must not sit in the silicone mat.

10.5 Fuses

Fuses may only be replaced by authorized service technicians. Users must not replace the fuses.

The Eppendorf AG Performance Plans are available to service and certify your device.

The services offered include:

• Installation qualification (IQ) and operational qualification (OQ)

• Service

• Calibration and validation

Information on the Performance Plans can be found on our website www.eppendorf.com.

NOTICE! Leaks will occur in the vacuum chamber if the seal is not serviced To ensure that the vacuum chamber functions safely, the seal must be replaced after 300

applications.

Have the seal replaced by the authorized service partner after 300 applications.

Defective seals must be replaced.

113Maintenance

epMotion® 5075

English (EN)

10.6 Performing a firmware update

Prerequisites

• The firmware is located on a USB storage medium.


• The MultiCon PC is switched off.

1. Connect the USB storage medium to a USB port on the rear of the epMotion.

2. Switch on the epMotion.

The epMotion automatically installs the firmware.

3. Wait until the epMotion has emitted 3 short signal tones.

4. Switch off epMotion.

5. Remove the USB storage medium.

10.7 Checking the dispensing volume

The physical properties of a liquid affect the dispensing volume, e.g., viscosity, vapor pressure and surface

tension. When you use liquids with a density greatly different from that of water, the dispensing volume

may be incorrect.

Gravimetrically check the dispensing of the epMotion. Correct the dispensing volume if necessary.

Perform the following inspection:

Prerequisites

• Distilled water

• Test liquid

1. Call up the application.

2. Adjust the application to your labware and dispensing tool.

3. Weigh destination labware.

4. Fill the source labware with distilled water.

5. Carry out the application.

The destination labware is filled with water.

6. Weigh destination labware.

7. Calculate the volume of the water. Volume = mass : density.

NOTICE! Malfunctions due to defective firmware.

Only perform firmware updates after being requested to do so by Eppendorf AG.

Only use firmware that you have received from Eppendorf AG.

Observe the ambient temperature for the density specification. The density of water at 20°C is

approx. 0.9982 g/mL.


English (EN)114

8. Check to see if the dispensing tool dispenses liquid without errors. To do so, compare the calculated

volume of water with the dispensing volume of the dispensing tool.

9. Repeat the check with test liquid and new labware.

10.Calculate the volume of the test liquid. Volume = mass : density.

11.Compare the volume of the water with the volume of the test liquid.

12.Change the dispensing volume if necessary.

10.8 Maintaining the dispensing tools

Changing the density by 10% will change the volume by approx. 0.2% - 1%.

NOTICE! Incorrect dispensing due to missing service.To ensure a dispensing procedure as specified, every dispensing tool must be serviced after

100,000 full strokes or 200,000 strokes.

When a dispensing tool has reached 100,000 full strokes or 200,000 strokes, the epBlue

displays a message.

Have the dispensing tool serviced after 100,000 full strokes or 200,000 strokes.

Send the dispensing tool to the authorized service partner.

115Maintenance

epMotion® 5075

English (EN)

10.8.1 Exchanging the sealing rings on the eight-channel dispensing tool

Exchange the sealing rings at the described intervals and if the following problems occur:

• Sealing rings are damaged.

• Tips are not attached in parallel.

• Tips are dripping.

• Once a year.

Abb. 10-2: Exchanging the sealing rings

Fig. 10-2: Exchanging the sealing rings

Exchange the sealing rings as follows:

Prerequisites

• New sealing rings

• Auxiliary tool

• Mounting aid

• Damp, lint-free cloth

1. Place the edge of the auxiliary tool at the height of the sealing ring.

2. Use the auxiliary tool to cut the sealing rings on the dispensing tool.

3. Remove the sealing rings with the fingers.

4. Clean all tip cones with a damp, lint-free cloth.

5. Attach the new sealing ring with the mounting aid.

6. Position the sealing rings in the grooves of the tip cones.



1 Sealing ring

2 Tip cone

3 Auxiliary tool

1

2

3


English (EN)116

10.9 Customer service functions

Customer service functions are available to service and check your device.

The following functions can be carried out:

The software operating manual contains a detailed description of the functions.

Customer service function Description

Optical sensor Define the functions of the optical sensor

Tool interlock Check the tool interlock mechanism.

Gripper Open and close the gripper.

Vacuum chamber Current vacuum in mbar.

Dosing device Read out the number of stroke movements of a dispensing tool.

ThermoMixer Check Eppendorf ThermoMixer module.

Thermal module Check the thermal module.

Activate and deactivate the temperature control.

117TroubleshootingepMotion® 5075

English (EN)

11 Troubleshooting

If you cannot remedy an error with the recommended measures, please contact your local Eppendorf

partner. The contact addresses can be found on the Internet at www.eppendorf.com.

11.1 Error search

If an application does not start, check the following items.

Device• Is the front hood of the epMotion or the safety glass of the Cleanbench completely closed?

Tools• Are the required dispensing tools available?

• Is the gripper available?

• Have the tools been correctly hung in the holder?

Worktable• Is the labware displayed in the software identical to the labware on the epMotion worktable?

• Is the labware at the location shown on the display?

• Are all locations which are shown as empty in the software actually empty on the epMotion worktable?

Labware• Is the labware correctly inserted?

• Is the labware inserted the wrong way around?

• Are you using the correct height adapter?

• Are all vessels open?

• Are the epT.I.P.S. Motion Racks sufficiently filled with pipette tips?

• Have the lids of the epT.I.P.S. Motion Racks removed?

• Are the lids of the Safe-Lock tubes positioned correctly?

• Are the minimum filling quantities in the source positions?

• Has the volume of the racks or plates, which will be required later, been entered in the application?

Waste container• Is the waste container empty?

• Is the disposal bag inserted correctly in the waste container?

• Is the clamping ring flush?

• Does the disposal bag protrude over the adjacent locations?• Is the bag able to hold the quantities of pipette tips required for the application?

Vacuum unit• Are all components of the vacuum unit inserted correctly?

Barcode reader• Is the barcode reader connected?

• Did you scan the intended ID?

• Did you select the Barcode-ID function in epBlue?

TroubleshootingepMotion® 5075

English (EN)118

11.2 General errors11.2.1 Optical sensor error

11.2.2 Dispensing error

Problem Cause Solution

The optical sensor

does not detect the

labware.

Plates are not positioned plane on the

worktable.

Plates were inserted in the wrong

direction.

Check to see if the labware is

correctly inserted in the locations.

The optical sensor

does not detect a

plastic plate.

Plastic surface is uneven. Wipe the plate several times using a

damp cloth.

Carry out a Location detection with a

damp plate.

The optical sensor

does not detect the

level.

The surface of the liquid is not plane. The

surface of the liquid is heavily curved.

• Smooth the surface of the liquid. To

do this, carefully tap the labware on

the table.

There are bubbles or foam on the surface

of the liquid.

• Remove bubbles or foam.


The dispensing tool

dispenses

incorrectly.

• The sealing rings on the dispensing

tool are broken.

Replace the sealing rings on the

dispensing tool.

Dispensing volume

too high.

During retraction, the opening of the

pipette tip is in the liquid in the source

position. There are no air bubbles on the

lower end of the pipette tip during liquid

transport.

• The actual volume in the source

position is higher than the entered

volume.

Measure volume with the optical

sensor.

Enter the correct volume.

Avoid different fill heights in the

positions of a plate.

• Liquid refilled after the application

was started.

Do not refill liquid after the

application has started.

• Incorrect labware used. Use labware that is defined in the

epBlue worktable.

Dispensing volume

too low.

The pipette tip draws in air. There are air

bubbles in the pipette tip.

• The actual volume in the source

position is lower than the entered

volume.

Measure volume with the optical

sensor.




• Incorrect labware used. Use labware that is defined in the

epBlue worktable.

119TroubleshootingepMotion® 5075

English (EN)

11.2.3 Messages from epBlue

For information on the epBlue software messages, refer to the software operating manual.

Residual liquid in

pipette tip.

• An incorrect volume was entered for

the destination position.

• After dispensing, liquid is withdrawn

from the destination position into the

pipette tip.

Use the optical sensor to determine

the volume.




• Incorrect liquid type selected.

• Unsuitable dispensing parameter.

Select a liquid type that matches the

liquid.

Optimizing dispensing parameters

(refer to the epMotion software

manual).

• Liquid wets the pipette tip.

• Liquid tends to foam.

Replace the pipette tip more often.

Optimizing dispensing parameters

(refer to the epMotion software

manual).


TroubleshootingepMotion® 5075

English (EN)120

121Transport, storage and disposal

epMotion® 5075

English (EN)

12 Transport, storage and disposal12.1 Transport

Use the original packing for transport.

The center of gravity of the device is at the rear.

Yellow carrying straps are attached to the device upon delivery.

Use the yellow carrying straps to lift the device out of the packing and position it.

12.2 Storage

12.3 Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations

based on EU Directive 2012/19/EU pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business

sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To

document this, they have been marked with the following identification:

CAUTION! Risk of injury when lifting and carrying heavy loadsThe device is heavy. Lifting and carrying the device can lead to back injuries.

Only lift and transport the device with a sufficient number of helpers.

Use a transport aid for transporting the device.

Air temperature Relative humidity Atmospheric pressure

General transport -25 °C – 60 °C 10 % – 75 % 30 kPa – 106 kPa

Air freight -20 °C – 55 °C 10 % – 75 % 30 kPa – 106 kPa

Air temperature Relative humidity Atmospheric pressure

In transport packing -25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa

Without transport

packing

-5 °C – 45 °C 10 % – 95 % 70 kPa – 106 kPa

Transport, storage and disposalepMotion® 5075

English (EN)122

Because disposal regulations may differ from one country to another within the EU, please contact your

supplier if necessary.

123Technical data

epMotion® 5075

English (EN)

13 Technical data13.1 Power supply

13.2 Ambient conditions

13.3 Weight/dimensions

13.4 Noise level

Voltage 100 V – 240 V ±10 %

Fuses 10 AT / 250 V

Frequency 50 Hz – 60 Hz ±5 %

Power consumption in standby mode 50 W

Power consumption maximum 700 W

Overvoltage category II (IEC 610 10-1)

Degree of contamination 2

Protection class 1

Ambience Only for use indoors.

Ambient temperature 15 °C – 35 °C

Relative humidity 55 % – 75 %, non-condensing.

Atmospheric pressure 86 kPa – 106 kPa

Dimensions Width: 107 cm (42.1 in)

Depth: 61 cm (24.0 in)

Height: 67 cm (26.4 in)

Height with CleanCap: 77 cm (30.1 in)

Weight epMotion 5075m: 88 kg (194.0 lb)

epMotion 5075t: 87 kg (191.8 lb)

epMotion 5075v: 86 kg (189.6 lb)

epMotion 5075vt: 90 kg (198.4 lb)

epMotion 5075I: 85 kg (187.4 lb)

CleanCap: 88 kg (194.0 lb)

Thermal module: 87 kg (191.8 lb)

: 86 kg (189.6 lb)

: 90 kg (198.4 lb)

Noise level typically 56 dB (A)

Technical dataepMotion® 5075

English (EN)124

13.5 Interfaces

Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected

to the interfaces.

13.6 MultiCon PC

You can find information on the technical data in the original operating manual of the MultiCon PC.

13.7 Additional specifications13.7.1 Optical sensor

13.7.2 Carrier

13.7.3 Gripper

USB USB 2.0

Ethernet Ethernet 100 MBit/s

Optical confocal infrared sensor Contact-free detection of

• Filling level in the labware

• labware code and height

• Number and location of the epT.I.P.S. pipette

tips. Motion in labware from Eppendorf

Detection conditions 90 ± 3° liquid surface to the optical axis

Minimum filling level 3 mm

Positioning accuracy along the X, Y and Z axes

Systematic deviation ±0,3 mm

Random deviation ±0.1 mm

Workspace Width: 700 cm (275.6 in)

Depth: 300 cm (118.1 in)

Height: 200 cm (78.7 in)

Form-fit carrying capacity ≤2 000 g

Force-fit carrying capacity ≤1 200 g

125Technical data

epMotion® 5075

English (EN)

13.8 Enhancements13.8.1 Thermal module

13.8.2 Eppendorf ThermoMixer module

13.8.3 Magnetic finger module

13.8.4 Vacuum unit

Temperature control 0 °C – 110°C

Temperature control duration 1 min – 120 min

Heating rate 9°C/min

Cooling rate for cooling below ambient

temperature

4°C/min

Systematic error Temperature

4 °C

37°C

95°C

Error

±2°C

±1 °C

±2°C

Random error ±0.5 °C

Power consumption 60 W

Maximum load 1 000 g (2.2 lb)

Temperature control 4 °C – 95°C

Minimum temperature 15 °C below the ambient temperature

Heating rate 5°C/min

Cooling rate for cooling above ambient temperature 3°C/min

Cooling rate for cooling below ambient temperature 1°C/min

Rotational speed 300 rpm – 2 000 rpm

Mixing time 5 s – 120 min

Power consumption 105 W

Recommended separation period 2 min when using 2.0 mL Safe-Lock tubes

Delivery volume 35 standard liters per minute

Negative pressure 0.1 kPa – 85 kPa

Suction time 1 s – 35 min 59 s


English (EN)126

13.8.5 Barcode reader

13.8.6 HEPA filter

13.8.7 UV lamp

Type Manual barcode reader

Connection USB

Weight 110 g (0.24 lb)

Barcodes supported • linear: UPC, Code 39, Code 128, Interleaved 2 of

5, Codabar, GS1-DataBar, Code 93

• 2D: Data matrix, QR code, Micro-QR code, Aztec

code

• stacked: PDF 417, Micro-PDF 417, Composite

• postal: USPS-OneCode (4CB), POSTNET,

PLANET, Japanese Post, Australian Post, Royal

Mail, KIX code

Filter class according to EN 1822 E 10

Volume flow 70 - 120 m3/h

Replacement interval 1 year

Main wavelength 254 nm (UV-C)

Output 16 W

Voltage 49 V

Radiant flux after 100 h 3.9 W

Radiant intensity at a distance of 1 m 39 μW/cm2

Replacement interval 2 years

Irradiation period per cycle 15 min

127Technical data

epMotion® 5075

English (EN)

13.9 Dispensing tool errors13.9.1 Pipetting

Dispensing tool Volume rangePipetting

Testing volume

Maximum permissible errors

Error

Systematic error

Random error

± % ± µL ± % ± µL

TS 50 1.0 μL – 50 μL 1 μL

5 μL

25 μL

50 μL

±15.0

±5.0

±1.5

±1.0

±0.15

±0.25

±0.375

±0.5

±5.0

±3.0

±0.6

±0.4

±0.05

±0.15

±0.15

±0.2

TS 300 20 μL – 300 μL 20 μL

30 μL

150 μL

300 μL

±4.0

±3.0

±1.0

±0.6

±0.8

±0.9

±1.5

±1.8

±2.5

±1.5

±0.4

±0.3

±0.5

±0.45

±0.6

±0.9

TS 1000 40 μL – 1 000 μL 40 μL

100 μL

500 μL

1 000 μL

±5.0

±2.0

±1.0

±0.7

±2.0

±2.0

±5.0

±7.0

±1.5

±1.0

±0.2

±0.15

±0.6

±1.0

±1.0

±1.5

TM 50-8 1.0 μL – 50 μL 1 μL

5 μL

25 μL

50 μL

±25.0

±5.0

±2.0

±1.2

±0.25

±0.25

±0.5

±0.6

±10.0

±5.0

±1.2

±0.6

±0.1

±0.25

±0.3

±0.3

TM 300-8 20 μL – 300 μL 20 μL

30 μL

150 μL

300 μL

±10.0

±10.0

±2.5

±1.5

±2.0

±3.0

±3.75

±4.5

±4.0

±3.5

±0.8

±0.5

±0.8

±1.05

±1.2

±1.5

TM 1000-8 40 μL – 1 000 μL 40 μL

100 μL

500 μL

1 000 μL

±6.0

±3.0

±1.5

±0.8

±2.4

±3.0

±7.5

±8.0

±2.5

±1.5

±0.3

±0.15

±1.0

±1.5

±1.5

±1.5


English (EN)128

13.9.2 Dispensing

13.10 Test conditions for dispensing tool errors

Test conditions and test evaluation in accordance with ISO 8655, part 6. Test with analytical balance with

evaporation protection, approved by the board of weights and measures.

The errors were determined under the following conditions:

Dispensing tool Volume rangeDispensing

Testing volume

Maximum permissible errors

Error

Systematic error

Random error

± % ± µL ± % ± µL

TS 50 1.0 μL – 50 μL 5 μL ±5.0 ±0.25 ±12.0 ±0.6

TS 300 20 μL – 300 μL 30 μL ±3.0 ±0.9 ±5.0 ±1.5

TS 1000 40 μL – 1 000 μL 100 μL ±2.0 ±2.0 ±2.0 ±2.0

TS 50-8 1.0 μL – 50 μL 5 μL ±7.5 ±0.38 ±15.0 ±0.75

TS 300-8 20 μL – 300 μL 30 μL

150 μL

300 μL

±5.5 ±1.7 ±15.0 ±4.5

TS 1000-8 40 μL – 1 000 μL 100 μL ±1.0 ±1.0 ±6.0 ±6.0

Pipette tip epT.I.P.S. Motion

Liquid Water according to ISO 3696

Number of determinations 10

Ambient temperature 20 °C – 25 °C ± 0.5 °C

Liquid dispensing Free-jet dispensing

129Ordering information

epMotion® 5075

English (EN)

14 Ordering information14.1 Tools

14.2 epT.I.P.S. Motion14.2.1 epT.I.P.S. Motion Racks

Order no. (International)

Description

Single-channel dispensing tool TS 505280 000.010 Volume range 1 μL - 50 μL

Single-channel dispensing tool TS 3005280 000.037 Volume range 20 μL - 300 μl

Single-channel dispensing tool TS 10005280 000.053 Volume range 40 μL - 1000 μL

Eight-channel-dispensing tool TM 505280 000.215 Volume range 1 μL - 50 μl

Eight-channel-dispensing tool TM 3005280 000.231 Volume range 20 μL - 300 μL

Eight-channel-dispensing tool TM 10005280 000.258 Volume range 40 μL - 1000 μL

5075 774.003 Holder for 6 dispensing tools5282 000.018 Gripper5075 759.004 Gripper holder


Description

epT.I.P.S. Motion 50 µL10 racks with 96 tips each

0030 014.405 Eppendorf Quality

0030 015.207 Sterile

epT.I.P.S. Motion Filter 50 µL10 racks with 96 tips each

0030 014.413 PCR clean

0030 015.215 PCR clean and Sterile

epT.I.P.S. Motion 300 µL10 racks with 96 tips each


0030 015.223 Sterile

epT.I.P.S. Motion Filter 300 µL10 racks with 96 tips each

0030 014.456 PCR clean


epT.I.P.S. Motion 1 000 µL10 racks with 96 tips each


0030 015.240 Sterile

epT.I.P.S. Motion Filter 1 000 µL10 racks with 96 tips each

0030 014.499 PCR clean


Ordering informationepMotion® 5075

English (EN)130

14.2.2 epT.I.P.S. Motion SafeRacks

14.2.3 epT.I.P.S. Motion Reloads


Description

epT.I.P.S. Motion 50 µL10 SafeRacks with 96 tips each


epT.I.P.S. Motion Filter 50 µL10 SafeRacks with 96 tips each

0030 014.618 PCR clean

epT.I.P.S. Motion 300 µL10 SafeRacks with 96 tips each


epT.I.P.S. Motion Filter 300 µL10 SafeRacks with 96 tips each

0030 014.634 PCR clean

epT.I.P.S. Motion 1 000 µL10 SafeRacks with 96 tips each


epT.I.P.S. Motion Filter 1 000 µL10 SafeRacks with 96 tips each

0030 014.650 PCR clean


Description

epT.I.P.S. Motion Reloads 50 µL12 × 2 trays with 96 tips each


epT.I.P.S. Motion Filter Reloads 50 µL12 × 2 trays with 96 tips each

0030 014.430 PCR clean


epT.I.P.S. Motion Reloads 300 µL12 × 2 trays with 96 tips each


epT.I.P.S. Motion Filter Reloads 300 µL12 × 2 trays with 96 tips each

0030 014.472 PCR clean


epT.I.P.S. Motion Reloads 1 000 µL12 × 2 trays with 96 tips each


epT.I.P.S. Motion Filter Reloads 1 000 µL12 × 2 trays with 96 tips each

0030 014.510 PCR clean


epMotion® 5075

English (EN)

14.2.4 Adapter

14.3 Plates


Description

TipHolder5075 751.399 for epT.I.P.S. Motion Reloads

All twin.tec plates are available with ID on request.

For further information about other plates, please use our catalog or go to the webpage

www.eppendorf.com


Description

twin.tec PCR Plate 96, skirtedlow profile, wells colorless, 25 pieces

0030 128.648 border colorless

0030 128.656 border yellow

0030 128.664 border green

0030 128.672 border blue

0030 128.680 border red

twin.tec PCR Plate 96, skirtedWells black, 25 pieces


twin.tec PCR Plate 384wells colorless, 25 pieces





0030 128.540 border red

PCR-Cooler3881 000.015 Starter Set (1 × pink, 1 × blue)

3881 000.023 pink

3881 000.031 blue

twin.tec PCR Plate 96, semi-skirtedwells colorless, 25 pieces





0030 128.613 border red

twin.tec microbiology PCR Plate 96 semi-skirtedwells colorless, 10 pieces




English (EN)132

14.4 Reservoirs

14.5 Racks14.5.1 Racks for individual tubes

twin.tec PCR Plate 96 LoBind semi-skirtedwells colorless, 25 pieces


twin.tec real-time PCR Plate 96 semi-skirtedwells white, 25 pieces


0030 132.548 border white

0030 132.556 border black


Description

Reservoir for ReservoirRack ModulPCR clean, 10 × 5 pieces in bags

0030 126.505 30 mL

0030 126.513 100 mL

Reservoir 400 mL5075 751.364 10 pieces


Description

Racks for individual tubescannot be tempered, for use with Eppendorf vessels and glass or plastic test tubes

5075 792.109 Ø 12 mm × 60 mm max. length

5075 763.001 Ø 12 mm × 100 mm max. length

5075 792.087 Ø 13 mm × 60 mm max. length

5075 762.005 Ø 13 mm × 100 mm max. length

5075 792.060 Ø 14 mm × 60 mm max. length

5075 792.001 Ø 14 mm × 100 mm max. length

5075 792.044 Ø 15 mm × 60 mm max. length

5075 792.028 Ø 15 mm × 100 mm max. length

5075 776.006 Ø 16 mm × 60 mm max. length

5075 760.002 Ø 16 mm × 100 mm max. length

5075 775.000 Ø 17 mm × 60 mm max. length

5075 761.009 Ø 17 mm × 100 mm max. length


Description


epMotion® 5075

English (EN)

14.5.2 Thermoracks

Rackcannot be tempered, for 24 Safe-Lock tubes

5075 751.453 0,5 mL/1,5 mL/2,0 mL, with adapter for tubes für 0.5 mL

5075 751.275 1,5 mL/2,0 mL

Rackfor 24 HPLC vials

5075 792.125 Ø 12 mm × 40 mm max. length

Rackfor 96 conical tubes

5075 791.005 1.5 mL /2.0 mL

Rack Smart5075 790.009 for 16 SmartCycler test tubes

Rack LC for 96 LightCycler capillaries

5075 795.000 20 μL/100 μL

PrepRack for 24 Safe-Lock tubes 2 mL

5073 751.006 for nucleic acid preparation with MagSep kits

ReagentRack5075 751.429 to the reagent tray of the MagSep Kits


Description

Thermorack for 24 Safe-Lock tubes

5075 769.000 0.5 mL

Thermorackfor 24 Safe-Lock tubes

5075 771.004 1.5 mL/2.0 mL

Adapterfor thermorack 1.5 mL/2.0 mL

5075 772.000 for holding Safe-Lock tubes 0.5 mL

Thermorack5075 777.055 for 24 Cryo tubes

Thermorack TMX for 24 Safe-Lock tubes

5075 751.160 0.5 mL

5075 751.186 1.5 mL/2.0 mL

Thermorack CBfor 384 tube strips

5075 767.031 100 μL

5075 751.526 Thermorack Rotor/TubesLid for Thermorack Rotor/Tubes

5075 751.640 with adapter for placing the lid on the epMotion worktable


Description


English (EN)134

14.5.3 ReservoirRacks

14.5.4 ReservoirRack modules

14.6 Adapter 14.6.1 Height adapter


Description

ReservoirRack5075 754.002 for 30 mL and 100 mL reservoirs and ReservoirRack Module TC

ReservoirRack 35075 754.070 for 30 mL and 100 mL reservoirs


Description

ReservoirRack Module TCtemperature-controlled, usable in ReservoirRacks, for the following tubes

5075 799.049 8 PCR micro test tubes 0.2 mlL

5075 799.081 4 Safe-Lock tube 1.5 mL/2 mL

5075 799.103 4 test tubes 12 mm diam.




5075 799.146 1 Reservoir 30 mL

5075 799.260 1 Reservoir 100 mL

5075 799.340 4 Eppendorf Tubes 5.0 mL


Description

Height adapter5075 751.003 85 mm

5075 752.000 55 mm

5075 755.009 40 mm


epMotion® 5075

English (EN)

14.6.2 Thermoadapter

14.7 Thermoblocks


Description

Thermoadapter5075 787.008 for PCR plates, 96 wells, skirted

5075 788.004 for PCR plates, 384 wells, skirted

Thermoadapter DWP 965075 751.054 for Eppendorf Deepwell-Plates 96/1000 μL

Thermoadapter LC Sample5075 751.305 for MagNA Pure LC sample cartridge

Thermoadapter FrostyCombination of height adapter and PCR cooler

5075 789.000 for PCR plates, skirted

Thermoadapter Microplate 96/V/U5075 751.577 for Microplate 96/V/U


Description

Thermoblock5075 766.000 for 96 test tubes 0.2 mL, 77 PCR tubes 0.5 mL or a PCR plate with 96 wells

5075 767.007 for a PCR plate with 384 wells

Thermoblock DWP 20005075 751.330 for Eppendorf Deepwell-Plates 96/2000 μL

5075 751.666 Thermoblock PCR 96 OC


English (EN)136

14.8 Accessories for the vacuum chamber

14.9 MagSep kits

14.10 Accessories

Worktable base adapters may only be mounted by authorized service technicians.


Description

Vac Holder5075 778.009 adapter for Vac Frame

Vac Lid5075 779.005 lid for vacuum unit

Vac Frame 15075 784.009 vacuum frame

Vac Frame 25075 785.005 vacuum frame

5075 793.008 Silicone mat for Vac LidElution plate adapter

5075 785.030 for the stabilisation of tube plates which are employed as collect vessel

5075 785.064 for vacuum unit

Channeling Platte 96adapter for the protection from contamination while working with foamy solutions

5075 794.004 10 pieces

Vac Thermo Lid5075 796.007 lid for vacuum unit


Description

MagSep Tissue gDNA Kit0030 450.000 Reagent kit for DNA purification of 4 × 24 tissue and cell samples

MagSep Blood gDNA Kit0030 451.007 Reagent kit for DNA purification of 4 × 24 blood samples

MagSep Viral DNA/RNA Kit0030 452.003 Reagent kit for viral DNA/RNA purification of 4 × 24 cell free biological fluid

samples.


Description

Waste box5075 753.006 for used pipette tips

LiquidWasteTub5075 751.500 to hinge in the waste container.

Worktable base adapterto raise the epMotion worktable

5070 752.001 4 pieces


epMotion® 5075

English (EN)

14.11 Modification kits and enhancements

Modification kits and enhancements may only be mounted by authorized service technicians.


Description

Thermal module5075 757.001 for heating or cooling the thermoadapter, thermoblock, or thermorack

Vacuum unit5075 000.610 to establish a vacuum on filter plates

5075 000.620 to establish a vacuum on filter plates

Eppendorf ThermoMixer module5075 000.630 to temper and mix liquids and suspensions

Extension plate5075 000.640 epMotion 5075vt

IndexepMotion® 5075

English (EN)138

Index

A

Accessories

Ordering information .....................................136

Adapter


Placement ......................................................100

Application

Abort ..............................................................104

Checking the labware ...................................117

Continue.........................................................103

Control ...........................................................103

Pause..............................................................103

Start................................................................102

Stop ................................................................103

Autoclaving..........................................................111

B

Barcode reader ......................................................48

Decontamination and cleaning ......................108

Technical data ................................................126

Bottom tolerance ...................................................52

C

Carrier

Technical data ................................................124

Channeling plate 96 ..............................................64

CleanCap ...............................................................46

Properties.........................................................28

Cleaning ......................................................107, 107

D

Danger symbol ......................................................19

Decontamination

Autoclaving ....................................................111

Spray decontamination ..................................110

UV irradiation ................................................111

Wipe decontamination ...................................109

Deepwell plate.......................................................63

Device

Mode of operation ........................................... 27

Switching off.................................................. 105

Switching on.................................................... 95

Dispensing ............................................................ 54

Checking the dispensing volume .................. 113

Procedure ........................................................ 51

Dispensing error ................................................. 118

Dispensing mode

Dispensing....................................................... 54

Mixing ............................................................. 56

Dispensing mode:

Pipette ............................................................. 54

Dispensing tool ..................................................... 49

Decontamination and cleaning...................... 108

Dispensing error............................................ 118

Exchanging the sealing rings........................ 115

Placement ........................................................ 98

Procedure ........................................................ 51

Disposal .............................................................. 121

E

EasyCon Tablet


Elution plate adapter............................................. 44

Eppendorf ThermoMixer module ......................... 38


Maximum speeds ............................................ 39

Properties ........................................................ 28

Technical data................................................ 125

epT.I.P.S. Motion................................................... 67

Adapter .......................................................... 131

Multiple use..................................................... 69

Ordering information..................................... 129

Placement ........................................................ 99

Rack......................................................... 68, 129

Reload...................................................... 70, 130

SafeRack .................................................. 69, 130

TipHolder......................................................... 71

Equipping the vacuum unit .................................. 45

Error message

Dispensing error............................................ 118

Optical sensor read error............................... 118

Software......................................................... 119


English (EN)139

F

Filter plate .............................................................63

Firmware

Update............................................................113

G

Gripper ..................................................................50


Placement ........................................................98

Properties.........................................................27

Technical data ................................................124

H

Height adapter

Description.......................................................84

HEPA filter.............................................................46

Technical data ................................................126

K

Keyboard

Use ...................................................................95

L

Labware

Bottom tolerance..............................................52


epT.I.P.S. Motion..............................................67

Height adapter .................................................84

LiquidWasteTub ...............................................92

Maximum speeds.............................................39

Placement ................................................96, 100

Plate .................................................................62

PrepRack ..........................................................75

Rack ...........................................................73, 74

ReagentRack ....................................................76

Reservoir ..........................................................65

ReservoirRack ..................................................80

ReservoirRack 3 ...............................................81

ReservoirRack module .....................................82

Thermoadapter ................................................85

Thermoadapter Frosty .....................................87

Thermoadapter Microplate 96/V/U..................86

Thermoblock ....................................................88

Thermoblock PCR 96 OC .................................89

Thermorack ..................................................... 77

TipHolder......................................................... 71

Tubes ............................................................... 61

Vac Holder....................................................... 85

Waste box ........................................................ 91

Liquid aspiration................................................... 52

From a filter plate ............................................ 59

From vessel base ............................................. 57

High vessels..................................................... 57

Liquid dispensing

At height of the edge of the vessel .................. 59

Principle .......................................................... 53

Liquid transport .................................................... 53

LiquidWasteTub.................................................... 92

Inserting the disposal bag ............................... 93

Placement ...................................................... 100

Location

Ports ................................................................ 29

Prerequisites.................................................... 29

M

Magnetic finger module ....................................... 40

Properties ........................................................ 28

Technical data................................................ 125

MagSepKit


Microplate............................................................. 63

Mixing liquids ....................................................... 56

Modification kit


Mouse

Use................................................................... 95

MultiCon PC


Technical data................................................ 124

N

Noise level .......................................................... 123


English (EN)140

O

Optical sensor........................................................34

Read error ......................................................118

Technical data ................................................124

Ordering information

Accessories ....................................................136

Adapter...........................................................134

epT.I.P.S. Motion............................................129

MagSepKit......................................................136

Modification kit ..............................................137

Plate ...............................................................131

Rack ...............................................................132

Reservoir ........................................................132

Thermoblock ..................................................135

Tools...............................................................129

Vacuum manifold ...........................................136

P

PCR plate ...............................................................64

Performance Plans ..............................................112

Pipette tips

epT.I.P.S. Motion..............................................67

epT.I.P.S. Motion Rack .....................................68

epT.I.P.S. Motion Reload..................................70

epT.I.P.S. Motion SafeRack ..............................69

Multiple use .....................................................69

Placement ........................................................99

TipHolder .........................................................71

Pipetting ................................................................54

Placement

Adapter...........................................................100

Dispensing tool ................................................98

Gripper .............................................................98

Plate ...............................................................100

Rack ...............................................................100

ReservoirRack module ...................................100

Tube ...............................................................100

Waste container .............................................100

Plate.......................................................................62

Channeling plate 96 .........................................64

Deepwell plate .................................................63

Filter plate........................................................63

Microplate ........................................................63


PCR plate..........................................................64

Placement ......................................................100

R

Rack

0.5/1.5/2.0 mL Rack......................................... 74


Placement ...................................................... 100

PrepRack ......................................................... 75

Rack for 24 vessels .......................................... 73

Rack for 96 conical vessels.............................. 73

Rack LC............................................................ 75

ReagentRack.................................................... 76

ReservoirRack.................................................. 80

ReservoirRack 3............................................... 81

ReservoirRack module..................................... 82

Remaining stroke.................................................. 55

Remaining volume ................................................ 52

Reservoir


Reservoir 100 mL ............................................ 65

Reservoir 30 mL .............................................. 65

Reservoir 400 mL ............................................ 66

ReservoirRack module

Placement ...................................................... 100

Reverse stroke ...................................................... 55

S

Safety device................................................... 18, 19

Service ................................................................ 112

Dispensing tool.............................................. 114

Dispensing tool sealing rings ........................ 115

Silicone mat .......................................................... 43

Spray decontamination ....................................... 110

Storage................................................................ 121

T

Technical data

Ambient conditions ....................................... 123

Barcode reader .............................................. 126

Carrier ........................................................... 124

Eppendorf ThermoMixer module.................. 125

Gripper .......................................................... 124

HEPA filter ..................................................... 126

Magnetic finger module ................................ 125

MultiCon PC .................................................. 124


English (EN)141

Noise level......................................................123

Optical sensor ................................................124

Thermal module.............................................125

UV lamp .........................................................126

Vacuum unit ...................................................125

Test run................................................................101

Thermal module ....................................................35


Equipping.........................................................37

Properties.........................................................27

Technical data ................................................125

Thermoadapter

Thermoadapter Frosty .....................................87

Thermoadapter LC Samples.............................86

Thermoadapter Frosty

Description.......................................................87

Preparation.......................................................87

Thermoadapter LC Samples

Description.......................................................86

Thermoadapter Microplate 96/V/U .......................86

Thermoblock .........................................................88


Preparation.......................................................88

Thermoblock PCR 96 OC.......................................89

Thermorack

0.5/1.5/2.0 mL thermorack...............................77

Thermorack TMX.............................................78

Tool

Dispensing tool ................................................49

Gripper .............................................................50

Placement ........................................................96

Tools



Touch screen

Application .......................................................95

Troubleshooting ..................................................117

Tube

Placement ......................................................100

U

UV irradiation......................................................111

UV lamp ................................................................ 47

Technical data................................................ 126

V

Vac Frame ............................................................. 42

Vac Holder

Description ...................................................... 85

Vac Lid .................................................................. 42

Vac Thermo Lid..................................................... 43

Vacuum frame....................................................... 42

Vacuum unit.......................................................... 41


Properties ........................................................ 28

Technical data................................................ 125

W

Waste box ............................................................. 91

Waste container

LiquidWasteTub............................................... 92

Waste box ........................................................ 91

Wipe decontamination........................................ 109

Workflow............................................................... 96

Worktable

Cleaning up ................................................... 104


Gripper placement........................................... 98

Locations ......................................................... 31

Placement of tools and labware ................ 96, 97

Placing dispensing tools.................................. 98

Placing the LiquidWasteTub.......................... 100

Placing the waste container .......................... 100

Positioning labware ....................................... 100

Positioning the epT.I.P.S. Motion .................... 99

Worktable base adapter



English (EN)142

Certificates

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