Mandelic Marine Complex®

Skin Conditioning Formulationsand

30% & 45% Mandelic Acid Peel Solutions

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Mandelic Marine ComplexSkin Conditioning Formulations

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Biochemistry of Mandelic AcidMANDELIC ACID IS:

an 8-carbon aromatic* alpha hydroxy acid (AHA), named after theGerman “mandel” and derived from the hydrolysis of an extract ofbitter almonds

also known as:ALPHA-HYDROXYBENZENEACETIC ACIDCARBOXYLIC ACID

*a class of organic chemical compounds that contain one or more rings of carbon atoms (phenol ring)and undergo chemical reactions that are characteristic of benzene.

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Biochemistry of Mandelic AcidMANDELIC ACID:

Chemical formula - C8H8O3 or HOCH(C6H5)COOH

MANDELIC ACID STRUCTURE

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Biochemistry of Mandelic Acid

GLYCOLIC ACID:

A 2-carbon AHA derived from Sugar Cane

Chemical Formula - C2H4O3 or HOCH2COOH

Glycolic Acid Structure

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Biochemistry of Mandelic Acid

MALIC ACID:

A 4-carbon AHA tart tasting dicarboxylic acid, found in apples

Chemical Formula - HOOCCH2CHOHCOOH or C4H6O5

Malic Acid Structure

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Biochemistry of Mandelic AcidComparison of Mandelic Acid and Glycolic Acid

Molecules:

Mandelic Acid: is stronger (pK 3.41) than Glycolic acid (pK 3.83) at 25℃ is larger (C8) than Glycolic Acid Molecule (C2) exhibits anti-microbial properties Is absorbed through epidermis at a slower and more even rate does NOT disrupt production of melanin pigment does NOT cause postinflammatory hyperpigmentation significantly improves melasma, postinflammatory hyperpigmentation,

and lentigenes

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Biochemistry of Mandelic AcidComparison of Mandelic Acid and Glycolic Acid

Molecules:Mandelic Acid: does NOT cause irritation or erythema as is seen with 30%-70% glycolic

acid preparations

peel preparations do not produce erythema, crusting or blistering

may be used safely on darkly pigmented skin types compared to glycolicacid and tretinoin

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BackgroundMandelic Acid Has a long history of use in the medical community as an antiseptic,

most commonly used to to irrigate the bladder for urinary tractinfections.

Methenamine Mandelate, best known as an anti-infective, available only by prescription Chemical structure (C6-H12-N4.C8-H8-O3), is similar to that of

other well-known antibiotics.

Medical Origins of Mandelic Acid

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Medical Origins of Mandelic AcidBackground

Methenamine Mandelate inhibits

Staphylcoccus aureusBacillus proteusEscherichia coliAerobacter aerogenes

Methenamine (meth-EN-a-meen) is nontoxic. After oral ingestion, it is rapidly absorbed and excreted in the urine. Through acid hydrolysis, bactericidal levels from methenamine are

rapidly reached at pH 5.0-5.5.

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Use of Mandelic Acid in DermatologyWhy the interest in including Mandelic Acid in Skin

Treatment Formulations?

Mandelic Acid is one of the strongest A.H.A.s. large molecular structure causes even penetration helps prevent inflammation and irritation that often occurs with

use of other AHA’s.

Mandelic acid (especially the 30% & 45% peel solutions) accelerates the cell renewal process, stimulates collagen synthesis, and improves elastin fibers in the dermis revitalizes sensitive skin helps improve the appearance of problematic skin.

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Use of Mandelic Acid in DermatologyWhy include Mandelic Acid in Dermatological Procedures? Great efficacy with ALL skin types

Absorbed through epidermis at a slower, even rate penetration into melanocytes does NOT occur disruption of the production of melanin does NOT occur lack of reactive hyper-pigmentation notable absence of post inflammatory hyper-pigmentation

Remarkable difference seen in the treatment of darkly pigmented skintypes of both Asian, African, and Hispanic origin

Considered to be the “New Generation Acid Peel for Dark, Sensitive SkinTypes.

Lack of any kind of hyper-pigmentation allows for a superior treatmentoutcome for ALL pigmented skin types

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Use of Mandelic Acid in Dermatology

Why include Mandelic Acid in Dermatological Procedures?

Hyperpigmentation most often referred to as Melasma occurs most often in women between 20 to 60 years of age affects ALL skin types exacerbated by exposure to sun, even in darker, pigmented skin types

Please see APPENDIX A - (slide 21) for more detail

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Use of Mandelic Acid in DermatologyWhy include Mandelic Acid in Dermatological Procedures?

Anti-microbial properties helpful in use of acid peels for treatment of acneprone skin

Mandelic Acid peel formulations do not contain any moisture lack of moisture and slower absorption allow for use of 30%-50% peels may be left on skin for a longer time without adverse side effects

Due to large aromatic phenol or carbon ring structure, Mandelic Acid: is an excellent accelerated exfoliation agent dissolves demosomes thus sloughing off necrosed squamous cells freshly exposed epidermal layers minimize appearance of wrinkles, age

spots and hyperpigmentation

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Use of Mandelic Acid in Dermatology

Mandelic Marine Complex® Skin Conditioning Systemsand Mandelic Acid Peel Solutions are designed to functionin a synergistic manner, so that you, the MedicalProfessional, are able to provide a complete skin treatmentprotocol from start to finish for every aspect of your client’sdermatological care.

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Use of Mandelic Acid in Dermatology30% & 45% CHEMICAL PEEL SOLUTION PROTOCOLS

Series of protocols using 30% or 45% Mandelic Marine Complex® Acid SolutionsPrior to Procedure - preparation - two to four weeks Patient treated with Mandelic Marine Complex® formulations

Cleanser, Toner, Mandelic Serum, Moisturizer, Scrub, SunScreenPeel Procedure - application of 30% or 45% Peel Solutions Patient undergoes Mandelic Acid Peel procedure

Post procedure - healing - optional depending upon the degree of procedure. Application of Post Resurfacing Healing System (containing Aloe) to aid in

healing to re-epithelialization.Cleanser, Healing ointment (semi permeable) – multiple times daily

Post procedure – maintenance Patient resumes treatment with Mandelic Marine Complex® formulations Application of SunSense SPF30+ is highly recommended for ALL skin types

realizing the benefits of treatment with an AHA

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Use of Mandelic Acid in DermatologyBenefits of each Step:

Pre-procedure - Preparation of skin. Use the Mandelic Marine Complex® Skin Conditioning System for a full two

weeks prior to any Laser or Peel Procedure. Efficacy of Procedure will beenhanced.

Procedure❖ Mandelic Acid Peel Solution (no moisture) is non-irritating and non-sensitizing❖ Mandelic Acid Peel Solution can be administered once per week for six weeks.❖ Treatment of ACNE - Increases and speeds healing of acne❖ Treatment of Hyperpigmentation - Minimizes appearance of Hyperpigmentation❖ Treatment of Anti-Aging - Encourages collagen production, and therefore

decreases the appearance of fine lines

Post Procedure - Maintenance❖ Resume Mandelic Marine Complex® Skin Conditioning System in order to

maintain the results of the Peel Procedure.

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Use of Mandelic Acid in DermatologyWHY MANDELIC ACID ABOVE ALL OTHER SKIN FORMULATIONS?

LARGER MOLECULE (C8) than any other AHA slows rate of absorption intoepidermis, allowing for even penetration

NON-IRRITATING & NON-SENSITIZING for ALL skin types, especially darker,pigmented, and sensitive skin

HYPERPIGMENTATION - minimizes appearance of hyper-pigmentation, anddoes NOT cause hyper-pigmentation

ACNE - increases and speeds healing of acne. Acne at grade III or belowresponds most positively

ROSACEA - minimizes effects of unbalanced skin and resulting inflammationwhich can cause proliferation of demodex mites in the hair follicles of the skin.

ANTI-AGING - encourages collagen production, and therefore decreases theappearance of fine lines

Mandelic Acid Peel solutions produce less erythema than glycolic peels, and areless likely to result in crusting or blistering.

Mandelic Acid Peel Solutions without moisture can be administered once perweek before and after treatments for six weeks

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Use of Mandelic Acid in Dermatology

Three Features and Benefits OfMandelic Acid Peel Solutions

Mandelic Marine Complex® formulations and Mandelic Acid Peel Solutions are a superioralternative for all individuals with sensitive skin who cannot tolerate traditional AHAformulations, especially Glycolic Acid. Often this can be as much as 25% or more of yourclientele, thus making NuCèlle® an important addition to any product mix.

Consistent use of Mandelic Marine Complex® Skin Conditioning Systems and PeelSolutions provide a safe, non-surgical, but effective approach to the problems of Hyper-pigmentation (including Pregnancy Mask, Melasma), Rosacea, Acne, and the “ButterflyRash” associated with Lupus.

Mandelic Acid Peel Solutions are a non-sensitizing solution to systematically remove built-uplayers of sun damaged skin cells without the downtime of more invasive skincareprocedures.

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NuCèlle® Mandelic Marine Complex® SkinTreatment Formulations and Peel Solutions

Skin Conditioning System

Peel Solutions

Healing System

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Appendix A - Hyperpigmentation Is most often referred to as Melasma. occurs most often in women between 20 to 60 years of age affects ALL skin types. exacerbated by exposure to sun, even in darker, pigmented skin types

Hormonal changes appear to be a primary cause of increased hyperpigmentation, evenin darker, pigmented skin types, whether it is due to pregnancy, oral contraceptive therapyor even post-menopausal estrogen replacement therapy.

The process of melanin formation starts deep in the cell, where a gene makes anenzyme called tyrosinase. After that, chemical dominoes fall: tyrosinase changes tyrosineinto dopa, which turns into dopaquinone – and dopaquinone forms either black-brown“eumelanin” or red-yellow” pheomelani”. These two types of melanin are formed asgranules within structures called melanosomes, which are then shipped off to epidermalcells at the surface of the skin.

Over time, observation was made that use of the Mandelic acid in the facial careformulations appeared to even out skin hyperpigmentation, and we began using theMandelic acid for the treatment of Melasma. Its mechanism of action in pigment reduction isnot well understood, but we hypothesize that it interferes with tyrosinase function, much likeasorbic acid. With the phenol side-chain, it may also interfere with o-quinones in themelanin pathway.

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For further reference, please contact:Deborah Fortescue-Merrin

deb@nucelle.comwww.nucellerx.com1+604-947-2894 Tel1+604-947-0294 Fax