m itochondrial genome replacement in unfertilized oocytes for treatment of inherited mt dna disease...
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MITOCHONDRIAL GENOME REPLACEMENT IN UNFERTILIZED
OOCYTESFOR TREATMENT OF INHERITED MTDNA
DISEASE
Shoukhrat Mitalipov
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Diseases caused by mtDNA mutations
There are more than 700 known disease-associated mtDNA mutations (mitomap.org):
- 285 tRNA/rRNA - 266 protein coding and control region point mutations; - 131 deletions
Acquired, age related - neurodegenerative diseases, Parkinson, heart diseases, diabetes, cancer
Inherited - neuropathy, encephalopathy, cardiomyopathy, myopathy, diabetes, metabolic syndromes
Up to 4,000 children are born in the United States every year with inherited mtDNA syndromes
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Complex nature of mtDNA genetics and inheritance
44% 25%
2% 85% 15% 52%
0% 2%
I
II
III
IV
Leber’s hereditary optic neuropathy (LHON)
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Inherited mtDNA diseases
mtDNA is maternally inherited - through the egg
Complex, unpredictable pattern of inheritance
These diseases are fatal or severely debilitating
No cure for mtDNA disease
Ultimate goal is to prevent transmission of mtDNA disorders by replacement of mutated genes in eggs
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Mitochondrial Gene Replacement in Oocytes
Complete replacement of entire mtDNA
Applicable to any mtDNA mutation type
Eliminates entire spectrum of mtDNA disease
Genetic corrections will be heritable and passed on to later generations
Prevents the need for repeated therapy generation after generation
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mtDNA replacement in oocytes
Feasibility and efficacy of MII spindle-chromosome complex transfer (ST)
Developmental Potential
Mutated mtDNA carryover
Nuclear/Mitochondrial genome compatibility?
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Mitochondrial gene replacement in oocytes
Spindle imaging
Separated chromosomes (nuclear DNA) and mitochondrial DNA
Distribution of mitochondria in mature oocytes
Spindle removal 7
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Mito & Tracker 8
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Cryopreservation of oocytes before ST
Tachibana et al., Nature, 20139
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Undetectable or low mtDNA carryover in tissues and organs of ST monkeys
Lee et al., Cell Reports, 201210
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mtDNA carryover in oocytes of ST monkeys
Lee et al., Cell Reports, 201211
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Normal growth and development of monkey offspring following mtDNA replacement
Tachibana et al., Nature 201312
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• 7 egg donors
• A total of 106 mature MII oocytes used for ST or served as controls
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mtDNA replacement by Spindle Transfer (ST) in human oocytes: efficacy, fertilization and embryo development
Tachibana et al., Nature 201314
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Fertilization outcomes in human zygotes following mtDNA replacement
Tachibana et al., Nature 201315
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ESC lines from human ST and control embryos
5 ESC lines from 13 human ST blastocysts (38%) contained normal euploid karyotypes
mtDNA carryover 1% or lower
1 ESC line from 6 abnormally fertilized ST blastocysts (17%) was triploid
9 ESC lines from 16 control blastocysts (56%), 2 cell lines were also karyotypically abnormal (XYY or X0)
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Conclusions
Entire cytoplasm containing mtDNA in human oocytes can be efficiently replaced by ST
Use of mt genome from donor egg (not recombinant)
Applicable to any mtDNA mutation type
ST is feasible with cryopreserved eggs
A portion of manipulated oocytes displayed abnormal fertilization
Normally fertilized zygotes develop to blastocysts and produce karyotypically normal ESCs at rates similar to controls
Thorough screening for abnormal fertilization is critical for selecting ST embryos for transfers
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Current efficiency allows generation of several (3-4) healthy embryos by ST suitable for embryo transfers for each cycle
Recruit families –carriers of early onset mtDNA diseases (at least one affected child, living or deceased)
Recruit healthy mtDNA egg donors
Conduct ST followed by PGD and/or prenatal diagnosis to ensure complete mtDNA replacement and chromosomal normalcy
Follow up with birth and development of healthy children
Clinical Trials
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