logistical considerations for participation in the psi-mr

Logistical Considerations for Participation in the PSI-MR

John G. Primm, Craig A. Bingman, Gary Wesenberg, Xiaokang Pan, Russell L. Wrobel, Kory D. Seder, Brian G. Fox, George N. Phillips, Jr.,

and John L. Markley

• Scope of CESG individual activity

• Current administrative process for CESG material distribution / Stakeholders

• New infrastructure developed for participation in PSI-MR

• Cost / Benefit analysis

• Considerations and Going Forward

PSI-2 Material Transfer Agreement Activity

0

5

10

15

20

25

30

35

Material Transfer Agreements Initiated

Year 1

Year 2

Year 3

Our most popular request…

CESG Materials Distribution PSI-2

Current Processes

Stakeholders

Step Action Cost Units Involved

1 Incoming request for material sent from Requestor to PI or comes in through CESG web site. $26.89 PI

2 Request is routed to PM office - preliminary review. $15.30 PI / PM

3 If the material can be shared, material location is extracted from LIMS and verified. $13.24 Cloning / PM

4Project Management office (PM) sets up files and contacts requestor to establishing legal signing entity for their institution. $4.64

PM office / Requestor

5Upon receipt of Legal information, a boiler plate MTA is form is filled out and UW routing forms are constructed; routing for execution begins. $21.02

PM assistant / PM / PI

6 The PI reviews and signs the documents. $15.30 PM / PI

7 Documents routed to UW Biochemistry chair for review / signature. Copies made and filed. $15.30 PM / Biochem

8Documents routed to UW College of Agriculture and Life Sciences (CALS) for review / signature. Entered into CALS database. Copies filed. $20.09

PM assistant / CALS intake /CALS

9Wisconsin Alumni Research Foundation (WARF) may be brought in depending on materials / issues. $3.09 CALS / WARF

10

After CALS / WARF review, documents are routed to UW Research and Sponsored Programs Office (RSP). MTA entered into RSP database. MTA reviewed and signed. Copies made and filed. $13.91 RSP intake / RSP

11 RSP routes to legal signatory for institutional signature. $28.91 RSP intake / RSP

12 Legal signatory routes to recipient scientist for external Requestor signature. $0.00Tech Transfer Office / Requestor

13 Executed copy returned to RSP, originals filed, RSP database status marked as "completed“. $10.43Tech Transfer Office / RSP

14 PM office notifies cloning; cloning pulls and prepares material. $54.94 Cloning / PM

15 Materials pass through QA step. $84.41 Cloning

16 Material is packed and sent to Requestor. $32.65 Cloning / PM

17 Specific information regarding the sample is sent to the Requestor. $13.24 Cloning

18Follow-up questions from the Requestor are directed to and answered by the appropriate CESG group. $26.47

Requestor / Cloning / PM

Total Cost for Average MTA and Transfer $399.80

Stakeholder IssuesIntellectual Property > UW licenses thousands of

products now in commerceLiabilityTracking of materials depositedTracking of materials distributedTracking of materials receivedOrganization and visibility of processesPermanent storage of recordsDistribution efforts

Physical / extraction of manipulation of data

Image from photobucket.com

Nuts

and

Bolts

Data First !

Plates last!

Clone Information Files (Required):

• Clone Location File

• Clone Information File

• Clone Gene Info File

Sesame actions required for internal tracking

Sesame search results

Human Eyes 2 hrs/WG

Permanent data storage

PSI-MR CESG Quarterly Report Order ID Order Date Status Clone ID987 FEB-06-2008 10:37:40 Shipped AtCD00082681983 FEB-04-2008 16:36:05 Shipped AtCD00082683

Species Specific ID User At1g70910 David AcetiAt4g16160 David Aceti

Shipping Information: CESG-QA 445 Henry Mall Madison, WI 53706 Phone: 608-890-0491User Email: [email protected] Name MARKLEY, John PI Institution Univ. Wisconsin-Madison PI Department Biochemistry PI Email [email protected]

mailto:[email protected]

mailto:[email protected]

Results to date

Viability of Destination Clones by Workgroup

0

20

40

60

80

100

WG

834

WG

875

WG

917

WG

925

WG

935

WG

975

WG

102

5

WG

119

1

WG

119

2

WG

119

7

WG

120

8

WG

123

0

WG

125

5

WG

126

5

WG

130

6

WG

132

2

WG

132

3

WG

138

5

Submitted

Transformed

% Transformed

CESG PSI-MR Deposition Progress as of 4/10/08

0200400600800

1000120014001600

TransformationAttempted

Transformed Accepted Rejected /mismatch

In Progress

De

sti

na

tio

n C

lon

es

Accepted Destination Clones By Workgroup

0

20

40

60

80

100

WG 8

34

WG 8

75

WG 9

17

WG925

WG 9

35

WG 9

75

WG 1

025

WG 1

191

WG 1

192

WG 1

197

WG 1

208

WG 1

230

WG 1

255

WG 1

265

WG 1

306

WG 1

322

WG 1

323

WG 1

385

% Transformed

% Accepted

Of the sample:

CESG was able to positively identify 83 % of those mismatched.

Pathway for remediation is under discussion.

Next GoalsReduce mismatched error rate 13.7% > by

remediation to 7.0%

Deposit all “declared available” destination clones by Oct 1. Or, better, before any changes in the data format occurs.

Improve CESG processes to include multiple vectors clones

Unit Estimated time HRS / 500 destination clones

Estimated cost / 500 destination clones

Projected costs - all remaining destination clones

Projected costs - all remaining entry clones

Bioinformatics 14 $711 $7,113 $1,423

Cloning 8 $424 $4,235 $5,929

QA 8 $349 $3,486 $697

CESG Admin 0.5 $31 $306 $428

RSP 0.1 $4 $42 $58

CALS 0.1 $5 $48 $67

Totals MR deposition 30.7 $1,523 $15,231 $8,604

Totals for traditional distribution $45,885

Projected Savings $22,051

Costs of ImplementationUnit Estimated time HRS Investment

Bioinformatics 280 $15,105.35

Cloning 80 $4,235.28

QA 20 $871.53

CESG Admin 45 $4,158.33

RSP 10 $417.22

CALS 18 $867.83

Cost of MR Participation Summary

Infrastructure Investment $24,700

Projected Savings ($22,000)

Priceless!

Looking Forward•PSI-MR will receive significant attention as useful biological research materials for biomedically relevant proteins whose purification properties and structures are known become available.

•Each Center preparing TargetDB files should already have accumulated all or nearly all of the information needed to prepare clone submission files for the PSI-MR.

•Opportunities are now emerging to create a wonderful synergy between the PSI-MR, PepcDB, and the PSI Knowledgebase.

– require that a unified, searchable tag is seeded into all three databases

Opportunity

Risk Requestors need and expect

all information required

(Protocols) to replicate your

success.

Data Preparation Depositors Agreement

Expedited MTA Covers Entire Institutions

Data Preparation Depositors Agreement

Dancing in the

Streets

Acknowledgements

CESG gratefully acknowledges the collaborative interactions with the following personnel of the PSI-MR as an essential part of this effort: Catherine Cormier, Joshua LaBaer, Stephanie Mohr, Andreas Rolfs, Elena Taycher, and Dongmei Zuo.

We also thank all members of CESG, including Arash Bahrami, Lenka Bittova, Lai Bergeman, E. Sethe Bergie, Christopher Bianchetti, Eduard Bitto, Claudia Cornilescu, Ronnie Frederick, Michael Goren, Katarzyna Gromek, Leigh Grundhoefer, Teagan Hayes, Andrew Larkin, Elena Levin, Betsy Lytle, Shin-Ichi Makino, John Markley, Yuko Matsubara, Karl Nichols, Xiaokang Pan, Francis Peterson, Mike Popelars, Sarata Sahu, Kory Seder, Donna Troestler, Frank Vojtik, Brian Volkman, Gary Wesenberg, Russell Wrobel, and Zsolt Zolnai for their support of this important initiative.