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LIS An Integrated Genomic and Gene2c Data Portal for the Legume Family (Legume Informa2on System)

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Page 1: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

L  I  S    

An  Integrated  Genomic  and  Gene2c  Data  Portal  for  the  Legume  Family    

(Legume  Informa2on  System)      

Page 2: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

Contributors  

Ethy  K.  S.  Cannon  Wei  Huang  Jacqueline  D.  Campbell  Vijay  Karingula  Andrew  P.  Wilkey  

Sudhansu  Dash  Alan  M.  Cleary1  Alex  G.  Rice  Pooja  E.  Umale  Andrew  D.  Farmer  1Montana  State  University  

 

Steven  B.  Cannon  ScoR  R.  Kalberer  Nathan  T.  Weeks  Jugpreet  Singh2                          2ORISE  Fellow    

Page 3: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

LIS  (hRp://legumeinfo.org)  is  a  resource  for  trait  gene2cs  and  compara2ve  genomics  for  legumes.  The  site  hosts  annotated  genomes  for  nine  species*.    LIS  leverages  data  from  informa2on-­‐rich  species,  such  as  soybean  and  Medicago,  to  aid  the  interpreta2on  of  data  from  other  species,  using  phylogene2c  and  synteny-­‐based  approaches.  Genes  from  hosted  genomes  have  been  placed  into  ~18,500  gene  families  which  enable  traversal  among  orthologous  and  paralogous  sequences  across  the  legumes.  This  is  complemented  by  func2onal  annota2ons  and  mul2-­‐species  microsynteny  views  showing  genomic  regions  with  similar  local  gene  content  and  ordering.      LIS  emphasizes  integra2on  of  gene2c  and  genomic  data.  QTLs  (so  far  from  common  bean  and  peanut)  have  integrated  into  a  common  database,  and  projected  onto  composite  gene2c  maps  (in  CMap).  Molecular  markers  are  being  mapped  on  to  the  genome  to  make  possible  traversal  from  traits  to  genome,  and  vice  versa.  *  common  bean,  chickpea,  pigeonpea,  Medicago  truncatula,  Lotus  japonicus,  mungbean,  soybean  (SoyBase.org)  and  two  Arachis    species  (PeanutBase.org).  

About  LIS  

Page 4: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

Can  I  locate  a  QTL  to  a  genomic  region  in  Phaseolus?  

Search  for  trait  ‘Seed  Weight’  

Page 5: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

QTL  Details  show  the  nearest  and  flanking  markers  

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The  three  markers  locate  the  ‘Seed  Weight’  QTL  to  approx.  1.1  Mb  region  on  Chr  02  in  the  Phaseolus  genomewithin  <  1  

Page 7: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

Can  I  trace  the  same    ‘Seed  Weight’  QTL  to  the  Soybean  Genome?  

Start  with  any  gene  in  the  region  to  find  orthologs  and  paralogs  in  its  gene  family  

Page 8: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

Select  a  soybean  gene  from  the  family  members  showing  microsynteny  with  others  

Page 9: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

The  soybean  gene  leads  to  the  syntenic  soybean  genomic  region    

Page 10: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

Use  any  marker  as  anchor  to  reach  the  soybean  gene2c  map    

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Seed  Weight  

Many  ‘Seed  Weight’  QTL  map  to  this  region  as  shown  in  the  soybean  composite  map  

Page 12: LISlegumeinfo.org/.../NAPIA2015/LIS_NAPIA_2016_sdash_POSTER_PRE… · LIS"(hRp://legumeinfo.org)"is"aresource"for"traitgene2cs"and"comparave"genomics" for"legumes."The"site"hosts"annotated"genomes"for"nine"species*."

•  LIS  is  a  community  resource  and  its  strength  depends  on  contribu2ons  from  community  members.  

 •  LIS  is  a  major  par2cipant  of  the  NSF  funded  

‘Legume  Federa2on’  project  which  is  developing  data  standards  and  templates  for  efficient  data  sharing.  

 •  Please  consider  contribu2ng  your  QTL,  mapping  

and  marker  data  to  LIS.  (Please  use  the  contact  form  at  our  website,  hRp://legumeinfo.org)  

A  Community  Resource