lead induces oxidative stress and phenotypic markers of apoptosis in saccharomyces cerevisiae...
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![Page 1: Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae (Applied Microbiology and Biotechnology) Jurrian Vanden Bussche](https://reader036.vdocuments.mx/reader036/viewer/2022082711/56649f115503460f94c238f0/html5/thumbnails/1.jpg)
Lead induces oxidative stress and phenotypic markers of apoptosis in
Saccharomyces cerevisiae
(Applied Microbiology and Biotechnology)
Jurrian Vanden Bussche and Eduardo V. Soares1
1Author for correspondence:
Eduardo V. Soares
Bioengineering Laboratory, Chemical Engineering Department, Superior
Institute of Engineering from Porto Polytechnic Institute,
Rua Dr António Bernardino de Almeida, 431,
4200-072 Porto,
Portugal
e-mail: [email protected]
Tel: 351-22-8340500
Fax: 351-22-8321159
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![Page 2: Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae (Applied Microbiology and Biotechnology) Jurrian Vanden Bussche](https://reader036.vdocuments.mx/reader036/viewer/2022082711/56649f115503460f94c238f0/html5/thumbnails/2.jpg)
Fig. S1. Membrane disruption in cells of S. cerevisiae NCYC 1214 heated at 65 ºC,
during one hour (control cells). Cells were stained with Oxonol or propidium iodide
(PI), as described in material and methods. Oxonol can freely enter in the cells with
depolarized membrane (a). Cells with compromised membrane incorporate PI (c).
Fluorescence micrographs (a and c); phase contrast micrographs of the same cells (b
and d).
a
c
b
d
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![Page 3: Lead induces oxidative stress and phenotypic markers of apoptosis in Saccharomyces cerevisiae (Applied Microbiology and Biotechnology) Jurrian Vanden Bussche](https://reader036.vdocuments.mx/reader036/viewer/2022082711/56649f115503460f94c238f0/html5/thumbnails/3.jpg)
Fig. S2. Membrane disruption in cells of S. cerevisiae NCYC 1214 heated at 65 ºC, during one
hour (control cells). A double staining protocol with Oxonol and PI was carried out, as
described in material and methods. Fluorescent micrographs of the cells observed with filter
set GFP (a) or I3 (b); phase contrast micrograph of the same cells (c).
a
b
c
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