lcd 10” touch screen - nisbets sheet hc027.pdf · lcd 10” touch screen turbovapor (*) just in...

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RESEARCH ARTICLE Proton and non-proton activation of ASIC channels Ivan Gautschi, Miguel Xavier van Bemmelen, Laurent Schild* Department of Pharmacology and Toxicology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland * [email protected] Abstract The Acid-Sensing Ion Channels (ASIC) exhibit a fast desensitizing current when activated by pH values below 7.0. By contrast, non-proton ligands are able to trigger sustained ASIC currents at physiological pHs. To analyze the functional basis of the ASIC desensitizing and sustained currents, we have used ASIC1a and ASIC2a mutants with a cysteine in the pore vestibule for covalent binding of different sulfhydryl reagents. We found that ASIC1a and ASIC2a exhibit two distinct currents, a proton-induced desensitizing current and a sustained current triggered by sulfhydryl reagents. These currents differ in their pH dependency, their sensitivity to the sulfhydryl reagents, their ionic selectivity and their relative magnitude. We propose a model for ASIC1 and ASIC2 activity where the channels can function in two dis- tinct modes, a desensitizing mode and a sustained mode depending on the activating ligands. The pore vestibule of the channel represents a functional site for binding non-proton ligands to activate ASIC1 and ASIC2 at neutral pH and to prevent channel desensitization. Introduction Acid-sensing ion channels (ASICs) are ligand-gated channels activated by protons. ASICs belong to the ENaC/degenerins family of ion channels that includes constitutively active channels, pep- tide-gated channels, and mechanosensitive channels. Six subtypes of ASIC are expressed in the vertebrate central and peripheral nervous systems. ASIC1 and ASIC3 are expressed in primary afferent sensory neurons where they initiate nociceptive signals in response to a drop in extracellu- lar pH associated with ischemic or inflammatory conditions [1, 2]. Beside its role in pain sensation, ASIC1 was reported to promote learning and fear conditioning [2]. ASIC-dependent excitatory postsynaptic currents (EPSCs) have been recorded in vivo although they remain quite small com- pared to glutamate-dependent EPSCs for instance [2]. ASIC channels are cation-selective and voltage-insensitive channels. The extracellular pH required for half-maximal activation of ASIC channels range from 4.5 to 6.8 depending on the subtype. The current elicited by protons is transient and followed by a desensitization that maintains the channel in a non-conducting state until the pH returns to physiological values. There is no specific high affinity pharmacological blocker of ASIC channels except toxins from spider (PcTx), sea anemone (APETx2), or snakes (mambalgin) [3][4][5]. MitTx, a toxin PLOS ONE | https://doi.org/10.1371/journal.pone.0175293 April 6, 2017 1 / 24 a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 OPEN ACCESS Citation: Gautschi I, van Bemmelen MX, Schild L (2017) Proton and non-proton activation of ASIC channels. PLoS ONE 12(4): e0175293. https://doi. org/10.1371/journal.pone.0175293 Editor: Alexander G Obukhov, Indiana University School of Medicine, UNITED STATES Received: September 2, 2016 Accepted: March 23, 2017 Published: April 6, 2017 Copyright: © 2017 Gautschi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: Data are available from the present study with DOI 10.5281/zenodo. 231036. Funding: This work was supported by the Swiss National Fund (www.snf.ch), grant number 310030_135378 to LS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have no competing interest.

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Page 1: LCD 10” Touch Screen - Nisbets sheet hc027.pdf · LCD 10” Touch Screen TURBOVAPOR (*) JUST IN TIME ECOSPEED ... R 1/2" EN10226 ISO 228.1 ... ø150 I dati riportati sul presente

LCD 10” Touch Screen

TURBOVAPOR (*)

JUST IN TIME

ECOSPEED

AUTOCLIMA

ECOVAPOR

FAST-DRY

CLOUD EVOLUTION

1/1 GN 530

325

7 x 1/1 GNNAGB071

DEVICE FOR GASTRONOMY

One TouchAutomatically and with only a singletouch, you can access the ideal and mostefficient cooking for each dish, due to theexclusive present and widely tested methods, times and system. A wide range of cooking methods is already tested in the field by a highly qualified group of workers.

(*) only for models with boiler)

Item

GFT(only for gas models)

MULTILEVEL

LCS CLEANING SYSTEMCOMBI CLEANCALFREE (*)

FLAVOURING

SYNCHRONISATION

SMOKING

CHEF PORTAL

REMOTE SERVICE

VIDEO RECIPES

COOKING MODES• ICS (Interactive Cooking System), an automatic cooking system for Italian,

French, International, Spanish, Russian, Asian and German recipes including their history, ingredients, procedure, automatic cooking program and plate presentation

• Manual cooking with three cooking modes: Convection from 30°C to 300°C, Steam from 30°C to 130°C, Combination Convection + Steam from 30°C to 300°C

• Programmable modes - Possibility to program and save cooking methods in an automatic sequence (up to 15 cycles) giving each program its own name, picture and information on the recipe

OPERATION• DISPLAY that can be configured based on the needs of the user, bringing

forward the programs used most• LAINOX Cloud Wi-Fi/Ethernet connection system for saving personal settings,

software updates, HACCP data archiving and downloading new recipes• Automatic “one touch” cooking (ICS) • Organization of recipes in folders with preview, giving each folder its own

name• Intelligent recognition of recipes in multilevel folders• 10” colour screen (LCD - TFT) that is high definition and capacitive with

“Touch Screen” functions• SCROLLER PLUS knob with Scroll and Push function for confirming choices• Automatic door opening using the “Open” button (optional)• Instantaneous display in ICS cooking of the HACCP graphic

Page 2: LCD 10” Touch Screen - Nisbets sheet hc027.pdf · LCD 10” Touch Screen TURBOVAPOR (*) JUST IN TIME ECOSPEED ... R 1/2" EN10226 ISO 228.1 ... ø150 I dati riportati sul presente

NAGB071

* Compared to our previous model

CLEANING AND MAINTENANCE• Self-diagnosis before starting to use the equipment, with descriptive and

audible signalling of any anomalies• Automatic LCS (Liquid Clean System) with COMBICLEAN liquid cleanser and

CALFREE liquid descaler, in 100 % recyclable cartridges• CALOUT descaling liquid system that prevents formation and accumulation of

limescale in the boiler, with separate container and automatic dispensing of CALFREE liquid descaling product

• Manual cleaning system with retractable hand shower

CONTROL EQUIPMENT• Autoreverse (automatic reverse of fan rotation) for perfect cooking uniformity• Automatic parallel compartment and core temperature control, DELTA T

system• Automatically regulated steam condensation• Programmable deferred cooking• Ability to choose up to 6 fan speeds; the first 3 speeds automatically activate

the reduction in heating power• For special cooking methods, you can use an intermittent speed• Temperature control at the product core using probe with 4 detection points• 2 core probe, a LAINOX exclusive• Core probe connection through a connector outside of the cooking chamber,

with the ability to quickly connect the needle probe for vacuum cooking and small pieces

• USB connection to download HACCP data, update software and load/unload cooking programs

• SERVICE program for: Inspecting the operation of the electronic board and display of the temperature probes - Appliance operation timer for all primary functions for programmed maintenance

• ECOSPEED - Based on the quantity and type of product, Naboo optimizes and controls the delivery of energy, maintains the correct cooking temperature, preventing any fluctuations (-10% energy / -30% water / -5% weight loss *)

• ECOVAPOR - With ECOVAPOR system there is a definite reduction in water and energy consumption due to the automatic controls of the steam saturation in the cooking chamber (-10% energy / -30% water / -10% steam *)

• TURBOVAPOR - With the TurboVapor system it automatically produces the ideal level of steam to cook “tough” products like egg pasta or asparagus, turnip greens and chard, which are notoriously fibrous vegetables

• GREEN FINE TUNING - A new burner modulation system and high efficiency exchanger to prevent power waste and reduce harmful emissions (-10% energy / -10% CO2 *)

CONSTRUCTION• IPX5 protection against sprays of water• Perfectly smooth, watertight chamber• Door with double tempered glass with air chamber and interior heat-reflecting

glass for less heat radiation towards the operator and greater efficiency• Internal glass that folds to open for easy cleaning • Handle with left or right hand opening• Adjustable hinge for optimal seal• Deflector that can be opened for easy fan compartment cleaning

GAS HEATING SYSTEM• Blown air type of modulating burners for the cavity • Automatic flame ignition • Electronic control of the flame with automatic ignition reset• High efficiency heat exchanger with steel AISI 310 S expansion cabinet

STEAM GENERATOR• AISI 316 fully insulated steam generator• Blown air type of modulating burners for the steam generator• Automatic boiler pre-heating• Daily automatic boiler draining (temperature of drained water below 60°C)

and rinsing• Alarm in case of boiler de-scaling required• Boiler semi-automatic de-scaling program• CAL-OUT system to prevent scale build-up within the boiler, as standard

SAFETY FEATURES• Cooking compartment safety thermostat• Cooking compartment safety device for pressure built-up and depression • Fan motor thermic protection• Flame detection sensor interrupting gas flow in case of switch of flame failure• Safety pressure switch for lack of gas alarm• Magnetic door switch• Lack of water displayed alarm• Self-diagnosis and fault display• Component cooling system with overheat display control• Two-stage gas valve• Boiler safety thermostat• Boiler water level probes• Boiler probe to check boiler completely empty

STANDARD EQUIPMENT• Automatic cleaning system LCS Liquid Clean System (equipped with 2 tanks

of COMBICLEAN detergent, 4,5 kg. each)• CALOUT descaling liquid system (equipped with 1 tank of anti-scale product

CALFREE, 4,5 kg. each) • Wi-Fi connection• Multipoint core probe (Ø 3 mm)• Built-in and retractable hand shower with tap • 2 stainless steel grids 1/1 GN

Page 3: LCD 10” Touch Screen - Nisbets sheet hc027.pdf · LCD 10” Touch Screen TURBOVAPOR (*) JUST IN TIME ECOSPEED ... R 1/2" EN10226 ISO 228.1 ... ø150 I dati riportati sul presente

GN kW

mm kW / kcal

kW / kcal

mm kW / kcal

mm kW

kg V - 50 Hz AC 230

0,5

10 / 8600

10 / 8600

0,5950 x 990 x 1000 h

15 / 12900

156 / 175

7 x 1/1

50 / 120

70

875 x 825 x 820 h

NAGB071

TECHNICAL DATA

Chamber capacity

Distance between layers

Number of meals

External dimensions

Dimensions with packing

Weight - Net / Gross

Total electric power

Gas versions nominal heating output

Chamber heating output

Steam heating output

Fan power

Power supply voltage

Special voltages and frequencies upon request

OPTIONAL - TO BE REQUESTED WHEN ORDERING OVEN• Automatic door opening - not compatible with handle (not available with

right hinged door) • Right hinged door• Configuration for connection to extraction hood• Extra charge for removable rack + compulsoring frame• Extra charge for pastry version removable rack 600 x 400 + compulsoring

frame• Extra charge for pastry version shelf racks x 600 x 400• SMOKEGRILL - 3 in 1 - Barbecue System / Smoking / Flavouring• Extra charge for Ethernet connection

ACCESSORIES• Multipoint core probe for large items. Pin length 180 mm. Ø 3 mm• Needle core probe - ø 1 mm.• Multiprobes system - 2 multipoint core probes - ø 3 mm.• Fat filter• USB pen to download HACCP data, update software and load/unload

cooking programs• Set of feet (h - 150 mm)• Stainless steel heat shield for the unit right side panel (essential when the

oven is positioned next to heat sources)

• Stainless steel floor stand• Special floor stands for insertion of the blast chiller• Side runners• Stainless steel shelf• Neutral cabinet with doors and side runners• Neutral cabinet with door• Static holding cabinet and slow cooking• Flanged feet kit for floor stands, for marine installations• Castor kit for floor stand, 2 with brake• Trolley for removable oven racks with drip tray• Removable rack (to be used with compulsoring frame)• Compulsoring frame for removable oven rack and plate racks• Pastry version - Removable rack 600 x 400 (to be used with compulsoring

frame)• Pastry version - Pair of shelf racks 600 x 400• Plate racks (to be used with compulsoring frame)• Rack thermal cover• Extraction hood with air-cooled condenser (available only for prearranged

ovens)

Page 4: LCD 10” Touch Screen - Nisbets sheet hc027.pdf · LCD 10” Touch Screen TURBOVAPOR (*) JUST IN TIME ECOSPEED ... R 1/2" EN10226 ISO 228.1 ... ø150 I dati riportati sul presente

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Dawson MMP Ltd Wath Road, Elsecar, Barnsley, South Yorkshire S74 8HJTel: 01226 350450 • Fax: 01226 350384e-mail: [email protected] • www.dawsonmmp.co.uk

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