late presentation with hiv in africa: … thank all the patients and staff from all the centres...
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![Page 1: LATE PRESENTATION WITH HIV IN AFRICA: … thank all the patients and staff from all the centres participating in the REALITY trial. Joint Clinical Research Centre, Kampala, Uganda:](https://reader036.vdocuments.mx/reader036/viewer/2022062909/5b91165b09d3f2857e8d6256/html5/thumbnails/1.jpg)
We thank all the patients and staff from all the centres participating in the REALITY trial. Joint Clinical Research Centre, Kampala, Uganda: P Mugyenyi, C Kityo, V Musiime, P Wavamunno, E Nambi, P Ocitti, M Ndigendawani; JCRC, Fort Portal, Uganda: S Kabahenda, M Kemigisa, J Acen, D Olebo, G Mpamize, A Amone, D Okweny, A Mbonye, F Nambaziira, A Rweyora, M Kangah, V Kabaswahili; JCRC. Gulu, Uganda: J Abach, G Abongomera, J Omongin, I Aciro, A Philliam, B Arach, E
Ocung, G Amone, P Miles, C Adong, C Tumsuiime, P Kidega, B Otto, F Apio; JCRC. Mbale, Uganda: K Baleeta, A Mukuye, M Abwola, F Ssennono, D Baliruno, S Tuhirwe, R Namisi, F Kigongo, D Kikyonkyo, F Mushahara, D Okweny, J Tusiime, A Musiime, A Nankya, D Atwongyeire, S Sirikye, S Mula, N Noowe; JCRC. Mbarara, Uganda: A Lugemwa, M Kasozi, S Mwebe, L Atwine, T Senkindu, T Natuhurira, C Katemba, E Ninsiima, M Acaku, J Kyomuhangi, R Ankunda, D Tukwasibwe, L Ayesiga; University of
Zimbabwe, Harare, Zimbabwe: J Hakim, K Nathoo, M Bwakura-Dangarembizi, A Reid, E Chidziva, T Mhute, GC Tinago, J Bhiri, S Mudzingwa, M Phiri, J Steamer, R Nhema, C Warambwa, G Musoro, S Mutsai, B Nemasango, C Moyo, S Chitongo, K Rashirai, S Vhembo, B Mlambo, S Nkomani, B Ndemera, M Willard, C Berejena, Y Musodza, P Matiza, B Mudenge, V Guti; KEMRI Wellcome Trust Research Programme, Kilifi, Kenya: A Etyang, C Agutu, J Berkley, K Maitland, P Njuguna, S Mwaringa, T Etyang, K
Awuondo, S Wale, J Shangala, J Kithunga, S Mwarumba, S Said Maitha, R Mutai, M Lozi Lewa, G Mwambingu, A Mwanzu, C Kalama, H Latham, J Shikuku, A Fondo, A Njogu, C Khadenge, B Mwakisha; Moi University Clinical Research Centre, Eldoret, Kenya: A Siika, K Wools-Kaloustian, W Nyandiko, P Cheruiyot, A Sudoi, S Wachira, B Meli, M Karoney, A Nzioka, M Tanui, M Mokaya, W Ekiru, C Mboya, D Mwimali, C Mengich, J Choge, W Injera, K Njenga, S Cherutich, M Anyango Orido, G Omondi Lwande, P
Rutto, A Mudogo, I Kutto, A Shali, L Jaika, H Jerotich, M Pierre; Department of Medicine and MLW Clinical Research Programme, College of Medicine, Blantyre, Malawi: J Mallewa, S Kaunda, J Van Oosterhout, B O'Hare, R Heydermann, C Gonzalez, N Dzabala, C Kelly, B Denis, G Selemani, L Nyondo Mipando, E Chirwa, P Banda, L Mvula, H Msuku, M Ziwoya, Y Manda, S Nicholas, C Masesa, T Mwalukomo, L Makhaza, I Sheha, J Bwanali, M Limbuni; MRC Clinical Trials Unit at UCL, London, UK:D Gibb,
M Thomason, AS Walker, S Pett, A Szubert, A Griffiths, H Wilkes, C Rajapakse, M Spyer, A Prendergast, N Klein. Independent REALITY Trial Monitors: F Kyomuhendo, S Nakalanzi, J Peshu, S Ndaa, J Chabuka, N Mkandawire, L Matandika, C Kapuya. Social Science Group: F Cowan, J Seeley, S Bernays, R Kawuma, Z Mupambireyi. Trial Steering Committee: I Weller (Chair), E Malianga, C Mwansambo, F Miiro, P Elyanu, E Bukusi, E Katabira, O Mugurungi, D Gibb, J Hakim, A Etyang, P Mugyenyi, J Mallewa.
Data Monitoring Committee: T Peto (Chair), P Musoke, J Matenga, S Phiri. Endpoint Review Committee: H Lyall (Co-Chair), V Johnston (Co-Chair), F Fitzgerald, F Post, F Ssali, A Prendergast, A Turkova, A Bamford, A Arenas-Pinto.
Funding: REALITY was funded by the UK Department for International Development (DFID), the Wellcome Trust and the Medical Research Council (MRC). Additional funding support was provided by the PENTA foundation. Merck Sharp & Dohme, Gilead Sciences, Cipla Ltd, ViiV Healthcare/GlaxoSmithKline donated drugs for REALITY and Valid International supplied Ready-to-Use-Supplementary-Food (RUSF).
Abraham Siika1, Leanne McCabe2, Mutsa Bwakura-Dangarembizi3, Cissy Kityo4, Jane Mallewa5, Kath Maitland6, Anna Griffiths2, Keith Baleeta7, Shepherd Mudzingwa3, James Abach8, Kusum Nathoo3, Andrew J. Prendergast9, A Sarah Walker2, Diana M Gibb2 and the REALITY Trial Team 1Moi University School of Medicine, Eldoret, Kenya; 2MRC Clinical Trials Unit at UCL, London, UK; 3University of Zimbabwe Clinical Research Centre, Harare, Zimbabwe; 4Joint Clinical Research Centre, Kampala, Uganda;
5Department/College of Medicine and Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi; 6KEMRI Wellcome Trust Research Programme, Kilifi, Kenya; 7Joint Clinical Research Centre, Mbale, Uganda; 8Joint Clinical Research Centre, Gulu, Uganda; 9Queen Mary University of London, London, UK
Contact details
Professor Diana Gibb,
MRC Clinical Trials Unit at UCL
90 High Holborn, Second floor
London WC1V 6LJ
email: [email protected]
Poster 896
Background: In sub-Saharan Africa, severely immunocompromized individuals are at high risk
of mortality during the first few months after starting ART. We aimed to determine predictors of
this early mortality and whether such “late presenters” could be grouped into phenotypes with
different mortality risks.
Methods: ART-naïve adults/children ≥5y with CD4<100 cells/ul initiating ART in Uganda,
Zimbabwe, Malawi and Kenya were included in the REALITY trial (ISRCTN43622374).
Baseline predictors of mortality through 48 weeks on ART were identified using Cox
regression with backwards elimination (exit p>0.1). Late presenter phenotypes were identified
using hierarchical clustering.
Results: Final multivariable models included 1711 participants (26<13y) of whom 203(12%)
died. Mortality was independently higher in those who were older (p=0.002), with lower CD4s
(p<0.001), lower albumin (p=0.001), lower haemoglobin (p=0.01) and weaker grip strength
(p=0.03); those in whom physicians reported WHO stage 3/4 weight loss (p=0.04); and in
those patients reporting fever (p=0.001), vomiting (p=0.02), some problems with mobility
(p=0.005) and inability to wash or dress themselves (p=0.003) at baseline. Five “late
presenter” groups were identified (figure), with mortality ranging from 4-25%. Group-1 had the
highest mortality (25%) and median CD4 28 cells/ul; they had a high burden of
symptoms/signs other than rash, weight loss, and problems with mobility and self-care; they
also had lower albumin and haemoglobin. Group-2 (11% mortality; median CD4 43 cells/ul)
had higher white blood cells, platelets and neutrophils, despite only 31% reporting infections
at baseline. Group-3 (10% mortality) were mainly younger women; they had similarly low
CD4s (median 27 cells/ul), haemoglobin and BMI to Group-1, but low symptom burden and
maintained fat mass. The remaining two groups had lower (4-6%) mortality, higher CD4
(median 42 and 48 cells/ul) and had predominantly maintained their weight within normal
ranges. Of note, the effect of the randomized enhanced prophylaxis bundle on mortality was
similar across all groups (interaction p=0.32).
Conclusions: Clinical and laboratory characteristics identified groups at highest risk of
mortality following ART initiation. A screening tool appropriate to the level of facility
could therefore help identify which patients with low CD4 counts should be prioritized,
e.g. for same-day ART initiation, more intensive follow-up, and enhanced prophylaxis.
ABSTRACT (updated) RESULTS
BACKGROUND
~20-25% of people starting ART in Africa have CD4 <100 cells/µL
- ~10% die within three months of starting treatment
Studies in “late presenters” are important to identify their key
characteristics, particularly if baseline CD4 count testing is not always
available, in order to identify them earlier at healthcare facilities and
prioritize them for additional interventions
In adults and older children enrolled in the REALITY trial, we
investigate in detail:
– Predictors of mortality in the first 48 weeks on ART
- Different patterns of “late presentation”
METHODS
REALITY (ISRCTN43622374) recruited 1805 HIV-
infected ART-naïve adults and children ≥5 years (98%
aged ≥13 years) from Zimbabwe, Uganda, Malawi and
Kenya with CD4<100 cells/µL
Patients initiated standard WHO-recommended
2NRTI+NNRTI with cotrimoxazole prophylaxis, and were
randomized using a factorial design to three different
additional 12 week interventions that might reduce early
mortality (enhanced antimicrobial prophylaxis, raltegravir,
ready-to-use supplementary food)
Baseline demographics, laboratory results (including VL
assayed retrospectively on stored samples), physical
LATE PRESENTATION WITH HIV IN AFRICA: PHENOTYPES, RISK AND RISK STRATIFICATION
http://www.ctu.mrc.ac.uk/our_research/research_areas/hiv/studies/reality/
ISRCTN43622374
measurements (including height, weight, MUAC,
body composition, blood pressure), physician
reported diagnoses (WHO stage 3/4 events),
participant-reported symptoms, EQ-5D scores and
randomized groups were all considered as
predictors of mortality during the first 48 weeks on
ART (using Cox models), after which participants
exited the trial.
– Factors were selected using backwards elimination (exit p>0.1
for an explanatory model). Clinical centre (reflecting
management and access to diagnostic facilities) was included
in all models which were restricted to complete cases.
Different patterns of “late presenters” were
identified using hierarchical cluster analysis.
Factor (effect in Cox models) Median (IQR) or
n (%)
Univariable effects of
factors selected for
multivariable model
Final multivariable
model in REALITY trial
participants (n=1711)
Multivariable model from
Bisson et al fitted to
REALITY trial participants
HR 95% CI P HR (95% CI P HR (95% CI P
Age (per 5 years older) 36 (29,42) 1.09 (1.03, 1.16) 0.005 1.12 (1.04, 1.20) 0.002 1.13 (1.06, 1.21) <0.001
CD4 (per 10 cells/mm3 higher) 37 (16,63) 0.89 (0.85, 0.94) <0.001 0.90 (0.85, 0.95) <0.001 0.87 (0.82, 0.92) <0.001
Haemoglobin (per g/dl higher) 11.2 (9.6,12.7) 0.77 (0.72, 0.82) <0.001 0.90 (0.83, 0.99) 0.01 0.87 (0.80, 0.94) <0.001
Albumin (per g/l higher) 35 (30,40) 0.92 (0.90, 0.93) <0.001 0.96 (0.94, 0.98) 0.001 0.94 (0.92, 0.97) <0.001
White blood cells (per 109/L higher) 3.5 (2.7,4.7) 1.17 (1.08, 1.26) <0.001 1.08 (1.00, 1.17) 0.054 1.16 (1.06, 1.25) 0.001
Grip strength (per kg higher) 24.5 (19.3,31.0) 0.93 (0.91, 0.95) <0.001 0.98 (0.96,1.00) 0.03 -
Previous healthcare contact † 163 (9%) 1.16 (0.75, 1.79) 0.50 0.63 (0.39, 1.03) 0.067 -
WHO 3/4 weight loss 327 (18%) 1.90 (1.42, 2.53) <0.001 1.43 (1.01, 2.03) 0.04 -
Patient-reported fever 240 (13%) 3.37 (2.54, 4.47) <0.001 1.67 (1.19,2.35) 0.003 -
Patient-reported vomiting 114 (6%) 3.72 (2.64, 5.26) <0.001 1.55 (1.00, 2.39) 0.048 -
EQ5D Mobility:
Some problems (vs none)
364 (20%)
4.42
(3.36, 5.81)
<0.001
1.88
(1.21, 2.93)
0.005
-
Confined to bed (vs none) 28 (2%) 11.87 (7.00, 20.1) <0.001 2.47 (1.00, 6.08) 0.050 -
EQ5D Self-care:
Some problems (vs none)
286 (16%)
3.73
(2.78, 5.02)
<0.001
1.32
(0.82, 2.13)
0.26
-
Unable to wash/dress (vs
none)
77 (4%)
8.59
(5.91, 12.5)
<0.001
2.78
(1.42, 5.43)
0.003
-
Enhanced prophylaxis 906 (50%) 0.75 (0.57, 0.97) 0.03 0.72 (0.54, 0.96) 0.02 -
Sex (female vs male) 961 (53%) 1.23 (0.95, 1.60) 0.12 - 1.19 (0.89, 1.60) 0.23
BMI: <18.5 (vs >25) 731 (41%) 2.41 (1.30, 4.48) 0.005 - 0.90 (0.52, 1.55) 0.70
18.5-25 (vs >25) 909 (51%) 1.38 (0.74, 2.58) 0.32 - 0.70 (0.41, 1.19) 0.19
HIV VL (per log10 higher) 5.4 (5.0,5.8) 1.52 (1.22, 1.89) <0.001 - 1.37 (1.09, 1.74) 0.008
Neutrophil % (per 5% higher) 50% (40,62) 1.07 (1.02, 1.12) 0.003 - 0.99 (0.95, 1.04) 0.64
87 45 25 12 15
268
349 217
206 383
0
50
100
150
200
250
300
350
400
450
Group-1 Group-2 Group-3 Group-4 Group-5
Nu
mb
er
of
pa
rtic
ipa
nts
Died AliveMortality
25% 11% 10% 6% 4%
Table 1: Independent predictors of mortality in the first 48 weeks on ART
initiated with CD4 <100 cells/mm3
† chronic health conditions or prescribed medications more than 14 days prior to screening visit. Note: final multivariable model in REALITY participants also adjusted for centre.
Figure 2: Characterisation of late presenter
“phenotypes” by baseline factors
Figure 1: Mortality by late presenter “phenotype”
CONCLUSIONS
Clinical and laboratory characteristics
identified groups of patients (all with
CD4<100 cells/mm3) at highest risk of
mortality following ART initiation
A screening tool appropriate to the level
of facility could therefore help identify
which patients with low CD4 counts
should be prioritized, e.g. for same-day
ART initiation, more intensive follow-up,
and enhanced prophylaxis
• 225 (12%) of 1805 participants starting ART with
CD4<100 cells/mm3 died before 48w
– median 8 weeks (IQR 3-18) on ART
• Mortality was independently higher in those who
were older, with lower CD4s, lower albumin,
lower haemoglobin and weaker grip strength;
those in whom physicians reported WHO stage 3/4
weight loss; and in those patients reporting fever,
vomiting, some problems with mobility and
inability to wash or dress themselves at
baseline (Table 1)
– Receiving enhanced-prophylaxis independently reduced
mortality (p=0.02) as in the trial overall (Hakim et al NEJM
2017 PMID 28723333), but receiving raltegravir (p=0.60)
and receiving ready-to-use-supplementary food (p=0.37)
did not
– The area under the receiver operating curve was 80%
(95% CI 76-83%) supporting good model discrimination
• VL was not an independent predictor once
problems with mobility were included as a
prognostic factor, suggesting mobility may be a
broad physical marker for the detrimental impact of
ongoing viral replication
• There was a marginal association between WBC
and mortality in our final model, supporting
infection and/or inflammation playing a role in early
increased mortality risk
• We were able to identify a more detailed and
slightly different set of clinical predictors to a recent
analysis of a smaller trial with lower mortality (7%)
(Bisson et al, AIDS 2017, PMID 28742529)
– High HIV VL and WBC, but not neutrophil percent, were
independent predictors of mortality in REALITY participants
when fitting the prognostic model from this previous study
(Table 1)
• However, other clinical factors were more
predictive than these laboratory parameters in
REALITY participants
• We then considered whether risk factors clustered
in specific groups of patients, that is, whether late
presenters could be phenotyped
• We identified 5 groups of patients (all with CD4<100
cells/mm3) whose mortality ranged from 25%
(Group-1) to 4% (Group-5) (Figure 1)
• Group-1 (25% mortality; median CD4 28 cells/mm3) had high
burden of problems reported on the EQ-5D, particularly for mobility,
self-care and usual activities (Figure 2). Excluding rash, they also
had the highest burden of symptoms and illness, especially weight
loss, difficulty walking and poor appetite
• Group-2 (11% mortality; median CD4 43 cells/mm3) had high levels
of WBC, platelets and neutrophils, despite lower levels of reported
fever (12%) than Group-1 (28%).
– Only 122 (31%) had an infection reported at enrolment,
suggesting they may have had underlying infections/inflammation
– This group also had substantial weight loss and low fat mass,
and low BMI, albumin and haemoglobin, similar to Group-1.
However, median CD4 was higher in Group-2 vs Group-1 as was
grip strength (median 24.5 vs 20.0kg)
• Group-3 (10% mortality; median CD4 27 cells/mm3) were mainly
(non-pregnant non-breastfeeding) females (76%) and younger
(median 30 vs 36 years overall). They had low CD4 counts, WBC,
neutrophils, haemoglobin, BMI, MUAC and grip strength, but
despite this had low burden of symptoms/ illnesses and maintained
a reasonable fat mass and higher albumin.
• Group-4 (6% mortality; median CD4 48 cells/mm3) was also mostly
female (97%), but older than Group-3 and much higher fat mass
BMI and MUAC
• Group-5 (4% mortality; median CD4 42 cells/mm3) was
predominately male (86%) and older (median 39 years) with higher
fat mass, fat free mass and grip strength