laboratory tuv sud south asia pvt. ltd., 373, udyog vihar ... · (conventional isolation method)...

Laboratory

TUV SUD South Asia Pvt. Ltd., 373, Udyog Vihar, Phase-II, Gurgaon, Haryana

Accreditation Standard ISO/IEC 17025: 2005

Discipline Biological Testing Issue Date 08.05.2015

Certificate Number T-2548 Valid Until 07.05.2017

Last Amended on 24.05.2015 of 61

S.No. Product / Material of Test

Specific Test Performed Test Method Specification against which tests are performed

Range of Testing / Limits of Detection

Rini Narayan Convenor

N. Venkateswaran Program Manager

I. FOOD AND AGRICULTURAL PRODUCTS

1. Animal Feeds

& Pet Foods

Aerobic Plate Count Compendium of methods for the

Microbiological examination of

foods, APHA (4th Edition) : 2001

Chapter 7 (Conventional Plate

count method)

>10 cfu/g

FDA- BAM online: 2001


count method)

>10 cfu/g

IS 5402: 2012

>10 cfu/g

Escherichia Coli by MPN

Technique

Compendium of Methods for the

microbiological examination of

foods,

APHA (4th Edition): 2001

Clause. 8.91 Chapter 8

>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002 Chapter 4

>3 MPN/g to

>1100 MPN/g

Enumeration of Escherichia

Coli

Compendium of methods for the


foods, APHA (4th Edition) : 2001

(MUG VRBA Method)

Clause. 8.933, Chapter 8

>10 cfu/g

Detection of Escherichia Coli IS 5887 (Part 1): 1976 (RA 2009)

Qualitative

(Present / Absent)/ g

Laboratory











Animal Feeds

& Pet Foods

Enumeration of Coliforms Compendium of methods for the


foods, APHA (4th Edition): 2001


(Conventional Solid medium

method)

>10 cfu/g

US FDA-BAM

Chapter 4, (Conventional MPN

method and Solid plate method)

>10 cfu/g

IS 5401 (Part 1): 2002

>10 cfu/g

Coliforms – MPN Technique

FDA- BAM: 2002 Chapter 4 3 MPN/g to

>1100 MPN/g




Clause. 8.71 & 8.72, Chapter 8

3 MPN/g to >1100

MPN/g

Detection of Shigella Spp Compendium of Methods for the



(cultural methods only)


Qualitative

(Present/Absent) /25 g

IS 5887 (Part 7): 1999 (RA 2009)

Qualitative

(Present / Absent)/25 g

Laboratory











Animal Feeds

& Pet Foods

Enumeration of Staphylococcus

Aureus




(Surface plating method)


>10 cfu/g

US FDA-BAM


Chapter 12

>10 cfu/g

IS 5887 (Part 8 /Sec I): 2002

(RA 2012)

>10 cfu / gm

Detection of Salmonella Spp Compendium of methods for the




Qualitative

(Present/ Absent )/ 25 g

IS 5887 (Part 3): 1999 (RA 2009)

Qualitative

(Present / Absent) /25 g

FDA- BAM: 2014 Chapter 5 Qualitative

(Present/ Absent)/25 g

Yeast and Mold Count Compendium of methods for the




>10 cfu/g

US FDA BAM: 2001

Chapter 18

>10 cfu/g

IS 5403: 1999 (RA 2009)

>10 cfu/g

Enumeration of Bacillus Cereus Compendium of methods for the



( Surface plating method)


>100 cfu/g

FDA- BAM: 2012


Chapter 14

>100 cfu/g

IS 5887 (Part 6): 2012 >100 cfu/g,

Laboratory











Detection of Listeria

Monocytogenes




(Conventional Isolation method)

Chapter 36

Qualitative



Qualitative


Animal Feeds

& Pet Foods

Enumeration of Clostridium

Perfringens



foods,APHA (4th Edition): 2001


>10 cfu/g,

FDA – BAM: 2001 Chapter 16

>10 cfu /g

Detection of Clostridium

Perfringens

IS 5887 (Part 4): 1999 (RA 2005)

Qualitative

(Present / Absent)/ 25 g

Detection of Vibrio Cholerae



foods, APHA(4th Edition): 2001


Qualitative



Qualitative


Enumeration of

Enterobacteriaceae



foods,


Clause. 8.62 ,Chapter 8

≥10 cfu/g

ISO 21528-2: 2004

≥10 cfu/g

Laboratory











2. Bakery and

Confectionery





count method)

>10 cfu/g



count method)

>10 cfu/g

IS 5402: 2012 >10 cfu/g

ISO 4833-1: 2013

>10 cfu/g


Technique




(Conventional MPN method)

Clause. 8.91,Chapter 8

>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002

Chapter 4

>3 MPN/g to

>1100 MPN/g


Qualitative





(Conventional Solid plate method)


>10 cfu/g

FDA- BAM: 2002 Chapter 4 >10 cfu/g

IS 5401 (Part 1): 2002 >10 cfu/g

IS0 4832: 2006

>10 cfu/g

Laboratory











Bakery and

Confectionery



3 MPN/g to

>1100 MPN/g

8.71 & 8.72, Chapter 8,




3 MPN/g to

>1100 MPN/g

Faecal Coliforms-– MPN

Technique

FDA- BAM: 2002

Chapter 4

3 MPN/g to

>1100 MPN/g



foods,


8.81 ,Chapter 8

3 MPN/g to

>1100 MPN/g


Aureus






>10 cfu/g

FDA- BAM : 2001

Chapter 12


>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu/g

ISO 6888-1: 1999

>10 cfu/g





Qualitative


Laboratory











Bakery and

Confectionery

FDA- BAM: 2014

Chapter 5

Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


Yeast and Mould Count Compendium of methods for the




>10 cfu/g

US FDA BAM: 2001

Chapter 18

>10 cfu/g

IS 5403: 1999 (RA 2009) >10 cfu/g

ISO 21527-1: 2008

>10 cfu/g


Perfringens





>10 cfu/g

FDA – BAM: 2001

Chapter 16,

>10 cfu/g


Perfringens

IS 5887 (Part 4): 1999 (RA 2005)

Qualitative


Enumeration of

Enterobacteriaceae





>10 cfu/g

ISO 21528-2: 2004

>10 cfu/g

Laboratory











Bakery and

Confectionery

Detection of Shigella Spp IS 5887 (Part 7): 1999 (RA 2009)

Qualitative







>100 cfu/g,

FDA- BAM: 2012

Chapter 14

>100 cfu/g,

IS 5887 (Part 6): 2012

>100 cfu/g

3. Canned Foods Total Plate Count Compendium of methods for the




Count method)

>10 cfu/g



count method)

>10 cfu/g

IS 5402: 2012

>10 cfu/g

Yeast & Mould Count Compendium of methods for the




>10 cfu/g

US FDA BAM: 2001

Chapter 18

>10 cfu/g

IS 5403: 1999 (RA 2009)

>10 cfu/g

Laboratory











Canned Foods Escherichia Coli by MPN

Technique






>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002

Chapter 4

>3 MPN/g to

>1100 MPN/g


Qualitative



Perfringens





>10 cfu/g

FDA – BAM: 2001

Chapter 16

>10 cfu/g


Perfringens

IS 5887 (Part 4): 1999 (RA 2005)

Qualitative







>100 cfu/g

FDA- BAM: 2012


Chapter 14

>100 cfu/g

IS 5887 (Part 6): 2012

>100 cfu/g,

Laboratory














(Conventional Solid plate method)


>10 cfu/g

FDA- BAM: 2002

Chapter 4

>10 cfu/g

IS 5401 (Part 1): 2002

>10 cfu/g


Aureus






>10 cfu/g

FDA- BAM: 2001


Chapter 12

>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu/g


Monocytogenes




Conventional Isolation method)

Chapter 36

Qualitative


FDA- BAM: 2013

Chapter 10

Qualitative


Thermophilic Flatsour

Sporeformers




Chapter 25.3

>10 spore / 10g

IS 2860: 1964 (RA 2001)

Passes the test

Laboratory











Canned Foods Detection of Shigella Spp IS 5887 (Part 7): 1999 (RA 2009)

Qualitative

(Present / Absent) / 25 g






>100 cfu/g,

FDA- BAM: 2012 Chapter 14 >100 cfu/g,

IS 5887 (Part 6): 2012 >100 cfu/g

ISO 7932: 2004

>100 cfu/g

4. Beverages

( Alcoholic & Non

Alcoholic)

Aerobic Plate Count IS 5402: 2012

>1 cfu/ml


>1 cfu/ml





count method)

>1 cfu/ml

ISO 4833 -1: 2013

>1 cfu/ml

Detection of Escherichia Coli

IS 5887 (Part 1): 1976 (RA 2005)

Present /Absent per 25 ml


Technique

FDA-BAM: 2002 (chapter 4) >3 MPN/g to

>1100 MPN/g

Laboratory











Beverages

( Alcoholic & Non

Alcoholic)





>3 MPN/g to

>1100 MPN/g


Coli (Β- Glucorinidase Positive)

ISO 16649 –2: 2001 >1 cfu/ml

Yeast and Mould Count IS 5403: 1999 (RA 2009) >1 cfu/ml

FDA-BAM: 2001 (chapter18) >1 cfu/ml





>1 cfu/ml

ISO 21527–1: 2008

>1 cfu/ml

Detection of Salmonella Spp. Compendium of methods for the




Qualitative

(Present/ Absent)/ 25 ml

FDA- BAM: 2014

Chapter 5

Qualitative

(Present/ Absent)/ 25ml

IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


ISO 6579: 2002

Qualitative


Laboratory











Beverages

(Alcoholic & Non

Alcoholic)

Detection of Shigella Spp






Qualitative


Enumeration of

Enterobacteriaceae

ISO 21528-2: 2004

≥1 cfu/ml





≥1 cfu/ml

Enumeration of Bacillus Cereus FDA-BAM: 2001(Chapter14) ≥100 cfu/ml



foods, APHA (4th Edition)

(Surface plating method): 2001


≥100 cfu/ml

IS 5887 (Part 6): 2012

≥100 cfu/ml


Aureus

FDA- BAM: 2001


Chapter 12

>1 cfu/ml




( Surface plating method): 2001


>1 cfu/ml

IS 5887 (Part 2): 1976 (RA 2005)

Qualitative

(Present/Absent)/25 ml

ISO 6888–1: 1999

>1 cfu/ml

Laboratory











Enumeration of Coliforms Compendium of Methods for the


foods APHA (4th Edition): 2001


>1 cfu/ml

FDA- BAM: 2002

Chapter 4,

>1 cfu/ml

Detection of Coliforms IS 5401 (Part 2): 2012 Qualitative

(Present/ Absent) /100 ml

Enumeration of Coliforms

ISO 4832: 2006 >1 cfu/ml,

Qualitative

(Present /Absent)/ 100 ml


FDA- BAM: 2002

Chapter 4

>(3 MPN to 1100 MPN)/

ml





>(3 MPN to 1100 MPN)/

ml

5. Cereal and Cereal

Products





count method)

>10 cfu/g

FDA- BAM: 2001

Chapter 3

>10 cfu/g

Laboratory











Cereal and Cereal

Products


Technique

IS 5402: 2012 >10 cfu/g

ISO 4833-1: 2013 >10 cfu/g





>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002

Chapter 4

>3 MPN/g to

>1100 MPN/g


Qualitative

(Present / Absent)



ISO 16649-2: 2001

>10 cfu/g

Coliforms & Faecal Coliforms Compendium of Methods for the



Clause. 8.71 & 8.72, 8.81

Chapter 8

>3 MPN/g to

>1100 MPN/g





>10 cfu/g


IS 5401 (Part 1): 2002

ISO 4832: 2006

>10 cfu/g






Qualitative

(Present/Absent)/ 25 g

IS 5887 (Part 7): 1999 (RA 2009)

Qualitative


Laboratory











Cereal and Cereal

Products


Aureus






>10 cfu/g

Detection of Staphylococcus

Aureus

IS 5887 (Part 2): 1976 (RA 2005)

Qualitative



Aureus

IS 5887 (Part 8/ Sec I): 2002

(RA 2012)

>10 cfu/g

ISO 6888-1: 1999

>10 cfu/g

Detection of Salmonella Spp Compendium of Methods for the




Qualitative

(Present/ Absent) / 25 g

IS 5887 (Part 3): 1999 (RA 2009)

Qualitative



ISO 6579: 2002

Qualitative


Yeast and Mold Count Compendium of Methods for the




>10 cfu/g

US FDA BAM: 2001

Chapter 18

IS 5403: 1999 (RA 2009) >10 cfu/g

ISO 21527-1: 2008

ISO 21527-2: 2008

>10 cfu/g

Laboratory











Cereal and Cereal

Products




Surface plating method)


>100 cfu/g


IS 5887 (Part 6): 2012

ISO 7932: 2004

>100 cfu/g


Monocytogenes





Qualitative



Qualitative


ISO 11290-1: 1996

Qualitative



Perfringens





>10 cfu/g


>10 cfu/g


Perfringens

IS 5887 (Part 4): 1999 (RA 2005)

Qualitative



Perfringens

ISO 7937: 2004 >10 cfu/g

Laboratory











Cereal and Cereal

Products

Detection of Vibrio Cholerae Compendium of Methods for the




Qualitative


IS 5887 (Part 5): 1976 (RA 2009)

Qualitative



Qualitative


Detection of Vibro

Parahaemolyticus

IS 5887 (Part 5): 1976 (RA 2009)

Qualitative



Qualitative


Enumeration of Lactic Acid

Bacteria

ISO 15214: 1998

≥10 cfu/g

Enumeration of

Enterobacteriaceae




Clause. 8.62,Chepter 8

≥10 cfu/g

ISO 21528-2: 2004 ≥10 cfu/g

6 Tea, Coffee and

Cocoa Products





count method)

>10 cfu/g

FDA- BAM: 2001 Chapter 3\

IS 5402: 2012 >10 cfu/g

ISO 4833-1: 2013

>10 cfu/g

Laboratory











Tea, Coffee and

Cocoa Products


Technique





>3 MPN/g to

>1100 MPN/g


3 MPN/g to

>1100 MPN/g


IS 5887 (Part 1): 1976 (RA 2009)

Qualitative



Coli(Β-Glucorinidase Positive)

ISO 16649 –2: 2001 >10 cfu/g

Enumeration of Coliforms ISO 4832: 2006 >10 cfu/g





>10 cfu/g


IS 5401 (Part 1): 2002 >10 cfu/g



3 MPN/g to

>1100 MPN/g





3 MPN/g to

>1100 MPN/g

Laboratory











Tea, Coffee and

Cocoa Products


Aureus






>10 cfu/g

FDA- BAM: 2001


Chapter 12

>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu/g

ISO 6888 – 1: 1999

>10 cfu/g





Qualitative



Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


ISO 6579: 2002

Qualitative

(Present / Absent)/25 ml





>10 cfu/g

US FDA BAM: 2001

Chapter 18

>10 cfu / g

IS 5403: 1999 (RA 2009) >10 cfu / g

ISO 21527–1: 2008

>1 cfu/g

Laboratory











Tea, Coffee and

Cocoa Products






>100 cfu/g

FDA- BAM: 2012

Chapter 14

IS 5887 (Part 6): 2012 > 10 cfu/g

ISO 7932 : 2004

> 10 cfu/g

Enumeration of

Enterobacteriaceae





> 10 cfu/g

ISO 21528-2: 2004 > 10 cfu/g


Monocytogenes





Qualitative


FDA- BAM: 2011

Chapter 10

Qualitative


IS 14988 (Part 1): 2001

Qualitative


ISO 11290-1: 2004

Qualitative



Perfringens





>10 cfu / g

FDA – BAM: 2001

Chapter 16

>10 cfu / g

Laboratory












Perfringens

IS 5887 (Part 4): 1999 (RA 2009)

Qualitative



Perfringens

ISO 7937: 2004 >10 cfu / g

Detection of

Vibrio Cholerae





Qualitative


FDA – BAM: 2004

Chapter 09

Qualitative


IS 5887 (Part 5): 1976 (RA 2009)

Qualitative


ISO 21872 – 1: 2007

Qualitative


Detection ofVibrio

Parahaemolyticus

FDA – BAM: 2004

Chapter 9

>3 MPN/g to

>1100 MPN/g

IS 5887 (Part 5): 1976 (RA 2009) Qualitative


ISO 21872 – 1: 2007

Qualitative


7. Fish and Sea

Foods





count method)

>10 cfu/g


IS 5402: 2012

>10 cfu/g

Laboratory











Fish and Sea

Foods


Technique





>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002 Chapter 4 >3 MPN/g to

>1100 MPN/g

Detection of Escherichia IS 5887 (Part 1): 1976 (RA 2009)

Qualitative






>10 cfu/g


IS 5401 (Part 1): 2002 >10 cfu/g



3 MPN/g to

>1100 MPN/g





3 MPN/g to

>1100 MPN/g





Chapter 38

Qualitative


Laboratory











Fish and Sea

Foods

IS 5887 (Part 7): 1999 (RA 2009)

Qualitative



Aureus






>10 cfu/g

FDA- BAM: 2001

Chapter 12


>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu / g


Aureus

IS 5887 (Part 2): 1976 (RA 2005)

Qualitative






Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


FDA- BAM: 2014

Chapter 5

Qualitative






>10 cfu/g

US FDA BAM: 2001

Chapter 18

>10 cfu/g

IS 5403: 1999 (RA 2009)

>10 cfu/g

Laboratory











Fish and Sea

Foods






>100 cfu/g,


>100 cfu/g


Monocytogenes




Chapter 36

Qualitative


IS 14988(Part 1): 2001 (RA 2012)

Qualitative



Qualitative



Perfringens




34.7, Chapter 34,

>10 cfu/g

FDA – BAM: 2001

Chapter 16


Perfringens

IS 5887 (Part 4): 1999 (RA 2009)

Qualitative


Detection of Vibrio Cholerae Compendium of Methods for the




Qualitative


FDA – BAM: 2004

Chapter 09

Qualitative




ISO 21872 –1: 2007

Qualitative


Laboratory











Fish and Sea

Foods

Enumeration of Vibrio

Parahaemolyticus

FDA – BAM: 2004 Chapter 09 >3 MPN/g to

>1100 MPN/g

Detection of Vibrio

Parahaemolyticus

IS 5887 (Part 5): 1976 (RA 2009)

Qualitative


ISO 21872 –1: 2007

Qualitative


Enumeration of

Enterobacteriaceae





>10 cfu/g,

ISO 21528-2: 2004

>10 cfu/g,

8. Fruit and Fruit

Products





count method)

>10 cfu/g


IS 5402: 2012

>10 cfu/g/ml

Incubation At 37°C For 10

Days

IS 2860: 1964

Changes in pH

Incubation At 55°C For 7 Days IS 2860: 1964

Changes in pH


Technique




Clause. 8.91 ,Chapter 8,

>3 MPN/g to >1100

MPN/g


>3 MPN/g to

>1100 MPN/g

Laboratory











Fruit and Fruit

Products


Qualitative






method)


>10 cfu/g


IS 5401 (Part 1): 2002

>10 cfu/g/ml

Coliforms by MPN Technique


3 MPN/g to

>1100 MPN/g




Clause. 8.71 & 8.72 Chapter 8

3 MPN/g to >1100

MPN/g






Qualitative


IS 5887 (Part 7): 1999 (RA 2009)

Qualitative

(Present/Absent)/ 25 g/ml


Aureus






>10 cfu/g

FDA- BAM: 2001


Chapter 12

Laboratory











Fruit and Fruit

Products


Aureus

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu / g


Aureus

IS 5887 (Part 2) : 1976 (RA 2005)

Qualitative

(Present / Absent)/

25 g or ml





Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative

(Present/Absent)/25 g/ml


Qualitative







Qualitative


IS 5887 (Part 5): 1976 (RA 2009)

Qualitative


FDA – BAM: 2004 Chapter 9 Qualitative


IS 5887 (Part 5): 1976 (RA 2009)

Qualitative



Parahaemolyticus


>1100 MPN/g

Detection of Vibrio

Parahaemolyticus



ISO 21872 –1: 2007

Qualitative


Laboratory











Fruit and Fruit

Products


Monocytogenes





Qualitative


IS 14988 (Part 1): 2001

(RA 2012)

Qualitative



Qualitative







>100 cfu/g,

FDA- BAM: 2012

Chapter 14

IS 5887 (Part 6): 2012

>100 cfu/g





>10 cfu / g,

US FDA BAM: 2001

Chapter 18

>10 cfu /g

IS 5403: 1999 (RA 2009) >10 cfu /g >1 cfu /g

Qualitative

(Present / Absent)/

25 g/ml


Perfringens





>10 cfu/g

Laboratory











Fruit and Fruit

Products


Perfringens

IS 5887 (Part 4): 1999 (RA 2009)

Qualitative



Perfringens


>10 cfu /g

Enumeration of Aciduric Flat

Sour Organisms





>10 cfu/g/ml


Bacteria





>10 cfu/g

Enumeration of

Enterobacteriaceae





>10 cfu/g,

ISO 21528-2: 2004

>10 cfu/g,

9 Herbs, Spices &

Condiments

Total Plate Count FDA- BAM online: 2001


count method)

>10 cfu/g





count method)

IS 5402: 2012

>10 cfu/g

Laboratory











Herbs, Spices &

Condiments

Yeast & Mould Count US FDA-BAM Chapter 18 >10 cfu/g





IS 5403: 1999 (RA 2009)

>10 cfu/g

Isolation & Enumeration of

Escherichia Coli

US FDA-BAM

Chapter 4


< 3 MPN/g

IS 5887 (Part 1): 1976 (RA 2009)

Qualitative





(MUG VRBA Method)


>10 cfu/g

Coliform Count US FDA-BAM

Chapter 4 (Conventional MPN


>10 cfu/g





method and Solid medium

method)

>10 cfu/g

IS 5401 (Part 1): 2012

(Solid medium Method)

>10 cfu/g

Laboratory











Herbs, Spices &

Condiments


Aureus

Chapter 12, US FDA-BAM


>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)






Detection of Salmonella Sp. US FDA-BAM

Chapter 5

Qualitative


IS 5887 (Part 3): 1999 (RA 2009)






Perfringens

US FDA-BAM Chapter 16 >10 cfu/g

IS 5887 (Part 4): 1999 (RA 2009)





Enumeration of

Enterobacteriaceae

IS/ISO 7402: 1993 (RA 2001) >10 cfu/g





Laboratory











Herbs, Spices &

Condiments

Enumeration of Bacillus Cereus US FDA-BAM


Chapter 14

>100 cfu/g

IS 5887 (Part 6): 2012 >10 cfu/g






>100 cfu/g

10 Weaning Foods

Detection of Coliform

IS 5401 (Part 2): 2002 Qualitative

(Present / Absent)/0.1 g

Detection of E.Coli

IS 5887 (Part 1): 1976 (RA 2009)

Qualitative



Aureus



Detection of Salmonella Spp IS 5887 (Part 3): 1999 (RA 2009)

Qualitative



Qualitative


Yeast and Mould Count

IS 5403: 1999 (RA 2009) ≥10 cfu/g

11 Meat and Meat

Products





count method)

>10 cfu/g


IS 5402: 2012 >10 cfu/g

ISO 4833 –1: 2013

>10 cfu/g

Laboratory











Meat and Meat

Products


Technique





>3 MPN/g to

>1100 MPN/g


Qualitative

(Present / Absent) /g or

25 g


Coli

ISO 16649 –1: 2001

>10 cfu/g,

Enumeration of Coliforms





>10 cfu/g

FDA- BAM: 2002

Chapter 4

>10 cfu/

IS 5401 (Part 1): 2002 >10 cfu/g

ISO 4832: 2006

>10 cfu/g






>3 MPN/ g to

1100 MPN/ g


Aureus



foods, APHA (4th Edition): 2001)(



>10 cfu/g

FDA- BAM: 2001


Chapter 12

>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu / gm

Laboratory











Meat and Meat

Products


Aureus

IS 5887 (Part 2) : 1976 (RA 2005) Qualitative



Aureus

ISO 6888 (Part 1): 1991 >10 cfu/g,





Qualitative


FDA- BAM: 2014

Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


ISO 6579: 2002

Qualitative






>10 cfu/g

US FDA BAM: 2001

Chapter 18

IS 5403: 1999 (RA 2009) >10 cfu/g

ISO 21527 – 1: 2008

>10 cfu/g


Monocytogenes





Qualitative


FDA- BAM: 2013 Chapter 10 Qualitative


IS 14988 (Part 1): 2001

Qualitative


ISO 11290 –1: 2004

Qualitative


Laboratory











Meat and Meat

Products


Perfringens





>10 cfu/g

IS 5887 (Part 4) : 1999 (RA 2009)

Qualitative


ISO 7937: 2004

> 10cfu/g

Enterococci Compendium of Methods for the


foods,APHA (4th Edition): 2001

Clause. 9.51.Chapter 9

>10cfu/g







Qualitative


IS 5887 (Part 7): 1999 (RA 2009)

Qualitative


ISO 21567: 2004 Qualitative


Enterobacteriaceae ISO 21528-2: 2004 >10 cfu/g





>10 cfu/g

Laboratory











Meat and Meat

Products






>100 cfu/g

FDA- BAM: 2012

Chapter 14

>100 cfu/g

IS 5887 (Part 6): 2012 >100 cfu/g,

ISO 7932: 2004

>10 cfu/g

12 Milk and Milk

Products




Conventional Plate count method)

Chapter 7

>10 cfu/g


IS 5402: 2012 >10 cfu/g

ISO 4833 – 1: 2013

>10 cfu/g


Technique





>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002

Chapter 4

>3 MPN/g to

>1100 MPN/g



ISO 16649 –2: 2001 >10 cfu/g


IS 5887 (Part 1): 1976 (RA 2009)

Qualitative


Laboratory











Milk and Milk

Products





>10 cfu/g


IS 5401 (Part 1): 2002 >10 cfu/g

ISO 4832: 2006 >10 cfu/g

ISO 4831: 2001 3 MPN/g to

>1100 MPN/g


FDA- BAM: 2002

Chapter 4

3 MPN/g to

>1100 MPN/g





3 MPN/g to

>1100 MPN/g

Faecal Coliforms Compendium of Methods for the




3 MPN/g to

>1100 MPN/g






Qualitative


IS 5887 (Part 7): 1999 (RA 2009)

Qualitative


ISO 21567: 2004

Qualitative


Laboratory











Milk and Milk

Products

Staphylococcus Aureus Compendium of methods for the





>10 cfu/g

FDA- BAM: 2001


Chapter 12

>10 cfu/g

ISO 6888 (Part 1): 1991 >10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu/g

IS 5887 (Part 2): 1976 (RA 2005)

Qualitative

(Present / Absent)/

0.1 g/1 g

Detection of Salmonella Spp





Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


FDA- BAM: 2014

Chapter 5

Qualitative


Detection of Salmonella Spp

ISO 6579: 2002

Qualitative


Enterobacter Sakazakii ISO 22964: 2006 Qualitative


Laboratory











Milk and Milk

Products





>10 cfu/ g

US FDA BAM: 2001

Chapter 18

>1 cfu/ ml >10 cfu/ g/ ml

IS 5403: 1999 (RA 2009) >10 cfu/g

Qualitative

(Present/Absent)/0.1 g

/1 g

ISO 21527 –1: 2008 >10 cfu/g

ISO 21527 –2: 2008

>10 cfu/g

Enumeration of Bacillus Cereus FDA- BAM: 2012

Chapter 14

>100 cfu/g

IS 5887 (Part 6): 2012

>1cfu/ml, >10 cfu/g

Enumeration of Bacillus Cereus ISO 7932: 2004

>10 cfu/g


Monocytogenes





Qualitative


FDA- BAM: 2013

Chapter 10

Qualitative


ISO 11290 –1: 2004 Qualitative

(Present / Absent)/g/

25 gm

IS 14988 (Part 1) : 2001 (RA

2012)

Qualitative


Laboratory











Milk and Milk

Products

Clostridium Perfringens Compendium of methods for the




>10 cfu/g

FDA – BAM: 2001

Chapter 16

>10 cfu/g

ISO 7937: 2004 >10 cfu/g

IS 5887 (Part 4): 1999 (RA 2009)

>10 cfu/g

Vibrio Cholerae Compendium of Methods for the




Qualitative


FDA – BAM: 2004

Chapter 09

Qualitative


Vibrio Parahaemolyticus FDA – BAM: 2004

Chapter 09

>3 MPN/g to

>1100 MPN/g

IS 5887 (Part 5): 1976 (RA 2009)

Qualitative


ISO 21872 –1: 2007

Qualitative


Enterobacteriaceae ISO 21528-2: 2004 >10cfu/g



foods,


>10cfu/g

Laboratory











13 Nut & Nut

Products

Aerobic Plate Count Chapter 7, Compendium of

methods for the Microbiological

examination of foods,


(Conventional Plate count

method) Clause. 8.62, Chapter 8

>10 cfu/g


IS 5402: 2012 >10 cfu/g

ISO 4833-1: 2013

>10 cfu/g


Technique





>3 MPN/g to >1100

MPN/g


>3 MPN/g to >1100

MPN/g


Qualitative(Present /

Absent)/ g


Coli

ISO 16649-2: 2001

>10 cfu/g





>10 cfu/g

FDA- BAM: 2002

Chapter 4

>10 cfu/g

IS 5401 (Part 1): 2002 >10 cfu/g

ISO 4832: 2006

>10 cfu/g

Laboratory











Nut & Nut

Products


FDA- BAM: 2002

Chapter 4

3 MPN/g to

>1100 MPN/g





3 MPN/g to

>1100 MPN/g

Enumeration of Bacillus Cereus FDA- BAM: 2012 Chapter 14 >100 cfu/g

IS 5887 (Part 6): 2012 >100 cfu/g

ISO 7932: 2004

>100 cfu/g






Absent)/25 g

IS 5887 (Part 3): 1999 (RA 2009)

Qualitative



Qualitative


ISO 6579: 2002

Qualitative


Yeast and Mold Count IS 5403: 1999 (RA 2009)





>10 cfu/g

ISO 21527-1: 2008

ISO 21527-2: 2008

>10 cfu/g

US FDA BAM: 2001

Chapter 18

>10 cfu/g

Laboratory











Nut & Nut

Products






Qualitative


IS 5887 (Part 7): 1999 (RA 2009)

Qualitative



Aureus






>10 cfu/g

FDA- BAM: 2001


Chapter 12

>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu / gm

ISO 6888-1: 1999

>10 cfu / gm


Perfringens





>10cfu/g

FDA – BAM: 2001

Chapter 16

>10 cfu /g


Perfringens

IS 5887 (Part 4): 1999 (RA 2005)

Qualitative



Perfringens

ISO 7937: 2004 >10 cfu /g

Laboratory











Enumeration of

Enterobacteriaceae





>10 cfu /g

ISO 21528-2: 2004

>10 cfu /g

Detection of Vibrio Cholerae FDA-BAM: 2004(chapter 9)

Chapter 40

Qualitative





Qualitative


IS 5887 (Part 5): 1976 (RA 2005)

Qualitative



Parahaemolyticus

FDA – BAM: 2004

Chapter 09

>3 MPN/g to

>1100 MPN/g

14. Sugar and Sugar

Products

Detection of Vibrio

Parahaemolyticus

IS 5887 (Part 5): 1976 (RA 2009)

Qualitative






count method)

>10 cfu/g


IS 5402: 2012 >10 cfu/g

ISO 4833-1: 2013

>10 cfu/g

Laboratory











Sugar and Sugar

Products


Technique





>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002

Chapter 4

>3 MPN/g to

>1100 MPN/g


IS 5887 (Part 1): 1976 (RA 2009)

Qualitative






>10 cfu/g

FDA- BAM: 2002

Chapter 4

>10 cfu/g

IS 5401 (Part 1): 2002 >10 cfu/g

IS0 4832: 2006 >10 cfu/g


FDA- BAM: 2002

Chapter 4

3 MPN/g to

>1100 MPN/g




Clause. 8.71, 8.72, Chapter 8

3 MPN/g to

>1100 MPN/g


Aureus






>10 cfu/g

FDA- BAM: 2001

Chapter 12


>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu/g

ISO 6888-1: 1999 >10 cfu/g

Laboratory











Sugar and Sugar

Products





Qualitative


FDA- BAM: 2014

Chapter 5

Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


Yeast and Mould Compendium of Methods for the




>10 cfu/g

US FDA BAM: 2001

Chapter 18

>10 cfu/g

IS 5403: 1999 (RA 2009) >10 cfu/g

ISO 21527-1: 2008

>10 cfu/g


Perfringens





>10 cfu/g

IS 5887 (Part 4): 1999 (RA 2005)

FDA – BAM: 2001

Chapter 16

>10 cfu/g

Enumeration of

Enterobacteriaceae





>10 cfu/g

ISO 21528-2: 2004

>10 cfu/g


Qualitative


Laboratory











Sugar and Sugar

Products




BAM ( Surface plating method


>100 cfu/g,

FDA- BAM: 2012

Chapter 14

>100 cfu/g,

IS 5887 (Part 6): 2012 >100 cfu/g

ISO 7932: 2004

>100 cfu/g


Bacteria

ISO 15214: 1998 >10 cfu/g

15. Snacks and

Instant Mixes

Total Plate Count FDA- BAM online: 2001


count method)

>10 cfu/g

IS 5402: 2012 >10 cfu/g





count method)

>10 cfu/g

Yeast & Mould Count US FDA-BAM

Chapter 18

>10 cfu/g

IS 5403: 1999 (RA 2009)




Chapter 20 Clause. 20.53

Laboratory











Snacks and

Instant Mixes

Isolation & Enumeration of

Escherichia Coli

US FDA-BAM

Chapter 4


3 MPN/g to

>1100 MPN/g

IS 5887 (Part 1): 1976 (RA 2009)

Qualitative





(MUG VRBA Method)


>10 cfu/g

Coliform Count US FDA-BAM



>10 cfu/g

IS 5401 (Part 1): 2012

(Solid medium method)

>10 cfu/g




(Conventional MPN method and

Solid medium method)

Chapter 8

>10 cfu /g


Aureus

US FDA-BAM

Chapter 12


>10 cfu /g

IS 5887 (Part 8/Sec 1): 2002

(RA 2012)






Laboratory











Snacks and

Instant Mixes

Enumeration of Bacillus Cereus US FDA-BAM


Chapter 14

>100 cfu /g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu /g




(Surface Plating method)


>100 cfu /g

Detection of Salmonella Sp. US FDA-BAM

Chapter 5

Qualitative

(Present / Absent) / 25 g

IS 5887 (Part 3): 1999 (RA 2009)





Detection of Shigella Sp. IS 5887 (Part 7): 1999 (RA 2009)

Qualitative






Clause. 38.21 , Chapter 38

Enumeration of

Enterobacteriaceae

IS/ISO 7402: 1993 (RA 2001)

>10 cfu /g





Laboratory











16. Vegetables and

Vegetable

Products





count method)

>10 cfu/g

FDA- BAM: 2001

Chapter 3

Aerobic Plate Count IS 5402: 2012

>10 cfu/g/ml

Incubation At 37°C For 10

Days

IS 2860: 1964

Changes in pH

Incubation At 55°C For 7 Days IS 2860: 1964

Changes in pH


Technique





>3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002

Chapter 4

>3 MPN/g to

>1100 MPN/g


Qualitative






>10 cfu/g

Laboratory











Vegetables and

Vegetable

Products

Enumeration of Coliforms FDA- BAM: 2002

Chapter 4

>10 cfu/g

IS 5401 (Part 1): 2002

>10 cfu/g/ml


FDA- BAM: 2002

Chapter 4

3 MPN/g to

>1100 MPN/g





3 MPN/g to

>1100 MPN/g






Qualitative


IS 5887 (Part 7): 1999 (RA 2009)

Qualitative

(Present / Absent)/25 g/

ml


Aureus



foods, APHA (4th Edition):2001



>10 cfu/g

FDA- BAM: 2001


Chapter 12

>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu / g

Laboratory











Vegetables and

Vegetable

Products

IS 5887 (Part 2): 1976 (RA 2005)

Qualitative

(Present / Absent)/

25 g or ml





Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative

(Present / Absent)/

25 g/ml

FDA- BAM: 2014

Chapter 5

Qualitative







Qualitative


IS 5887 (Part 5) : 1976 (RA 2009)

Qualitative

(Present/ Absent)/

25 g/ ml

FDA – BAM: 2004

Chapter 09

Qualitative


Detection of Vibrio

Parahemolyticus

IS 5887 (Part 5): 1976 (RA 2009)

Qualitative

(Present / Absent)/

25 g/ml


Parahemolyticus


>1100 MPN/g

Laboratory











Vegetables and

Vegetable

Products


Monocytogenes





Qualitative


FDA- BAM: 2013

Chapter 10

Qualitative







>100 cfu/g,

FDA- BAM: 2012

Chapter 14

>100 cfu/g

IS 5887 (Part 6): 2012

>100 cfu/g

Yeast and Mold





>10 cfu / g,

US FDA BAM: 2001

Chapter 18

IS 5403: 1999 (RA 2009)

>10 cfu / g


Perfringens

IS 5887 (Part 4): 1999 (RA 2005)





>10 cfu/g

FDA – BAM: 2001

Chapter 16

Laboratory











Vegetables and

Vegetable

Products

Aciduric Flat Sour Organisms Compendium of Methods for the




5 spore/ 10g


Bacteria





>10 cfu/g

Enumeration of

Enterobacteriaceae





>10 cfu/g,

ISO 21528-2: 2004

>10 cfu/g

16 Ready- To –Eat

Foods

Total Plate Count FDA-BAM online: 2001


Count Method)

>10 cfu/g

IS 5402: 2012 >10 cfu/g





count method)

>10 cfu/g

Yeast and Mold Count US FDA BAM: 2001

Chapter 18

>10 cfu/g

IS 5403: 1999 (RA 2009) >10 cfu/g





>10 cfu/g

Laboratory











Ready- To –Eat

Foods


Technique

US FDA- BAM , 2002


Chapter 4, Chapter 4,

FDA- BAM: 2002

3 MPN/g to

>1100 MPN/g


Coli




(MUG VRBA Method)


>10 cfu/g





method) Clause. 8.73, Chapter 8

>10 cfu/g





Chapter 8

3 MPN/g to

>1100 MPN/g

US FDA-BAM (Conventional

MPN method and Solid plate

method) Chapter 4

>10 cfu/g

IS 5401 (Part 1): 2002

>10 cfu/g


FDA- BAM: 2002

Chapter 4

3 MPN/g to

>1100 MPN/g





3 MPN/g to

>1100 MPN/g

Laboratory











Ready- To –Eat

Foods


Aureus






>10 cfu/g

US FDA-BAM


Chapter 12

>10 cfu/g

IS 5887 (Part 8/Sec I): 2002

(RA 2012)

>10 cfu / gm






>100 cfu/g

FDA- BAM: 2012


Chapter 14

>100 cfu/g

IS 5887 (Part 6): 2012

>100 cfu/g,



foods, APHA (4th Edition),2001


Qualitative


IS 5887 (Part 3): 1999 (RA 2009)

Qualitative


FDA- BAM: 2014

Chapter 5

Qualitative


Detection of Shigella Spp IS 5887 (Part 7): 1999

(RA 2009)

Qualitative


Laboratory











Ready- To –Eat

Foods





(Conventional Isolation method)



Absent)/25 g

Enumeration of

Enterobacteriaceae

ISO 21528-2: 2004

≥10 cfu/g





≥10 cfu/g

II. WATER

1. Drinking Water –

(Pipe and

Borewell )


IS 1622: 1981 (RA 2009) 1 MPN/100 ml

Detection of Escherichia Coli IS 1622: 1981 (RA 2009) Qualitative


E.Coli and Coliforms by

Membrane Filtration Technique

IS 15185: 2002 (RA 2007) /

ISO 9308-1: 2000

Qualitative

(Present/ Absent) /

100 ml or 250 ml

2. Packaged Natural

Mineral Water

and Packaged

Drinking Water


IS 5887 (Part 1): 1976 (RA 2009)

IS 15185: 2002 (RA 2007) /

ISO 9308-1: 2000

Qualitative

(Present/Absent)/ 250 ml

Detection of Coliform IS 5401: 2002

IS 15185: 2002 (RA 2007) /

ISO 9308-1: 2000

Qualitative


Detection of Faecal

Streptococci

IS 5887 (Part 2): 1976 (RA 2009)

Qualitative


Laboratory











Packaged Natural

Mineral Water

and Packaged

Drinking Water


Aureus

IS 5887 (Part 2): 1976 (RA 2009)

Qualitative


Detection of Sulphite Reducing

Anaerobes

IS 13428: 2005 Annexure C Qualitative

(Present / Absent)/ 50 ml


Perfringens

EC 98/83 Annexure 3 Qualitative

(Present /Absent)/ 100 ml

Detection of Pseudomonas

Aeruginosa

IS 13428: 2005

Annexure D

Qualitative


Detection of Yeast and Mold

IS 5403: 1999 Qualitative


Aerobic Microbial Count IS 5402: 2012 @ 22 °C & 37 °C

>1 cfu/ml

Detection of Salmonella

IS 5887 (Part 3): 1999 (RA 2009)/

IS 15187: 2002 (RA 2007)

Qualitative



Qualitative



and V.Parahemolyticus

IS 5887 (Part 5): 1976 (RA 2009)

Qualitative


3 Water For

Processed Food

Industry

Coliform IS 1622: 1981 (RA 2009)

>1 MPN/100 ml

Proteolytic & Lipolytic Count IS 4251: 1967 (RA 2009)

>1 cfu/ml

Laboratory











4. Drinking Water Detection of E.Coli IS/ ISO 9803-1: 2000 Qualitative


Detection of Enterococci

IS 15186: 2002 Reaffirmed 2007/

ISO 7899-2: 2000

Qualitative

(Present/Absent)/ 250 ml/

100 ml


Perfringens (Including Spores)

98/83/EC

Qualitative


Standard Plate Count

IS 1622: 1981 (RA 2009)

>1 cfu/ml

III. COSMETICS & ESSENTIAL OILS

1. Cosmetics/

Cosmetic Raw

Materials

(Shampoos,

Powders, Skin

Cream, Lotions,

Kajal, Lipstick,

Tooth Paste,

Tooth Powder,

Moisturizing

Cream, Soaps,

Baby Products,

Eye Products(In

and Around The

Eyes)

Total Microbial Count

(Included TPC and YMC)

IS 14648: 2011 >10 cfu/g

Gram Negative Pathogens



Detection of Pseudomonas




Aureus







count method)

≥10 cfu/g

FDA- BAM: 2001

Chapter 3

≥10 cfu/g

IS 5402: 2012

≥10 cfu/g

Laboratory











Cosmetics/

Cosmetic Raw

Materials

(Shampoos,

Powders, Skin

Cream, Lotions,

Kajal, Lipstick,

Tooth Paste,

Tooth Powder,

Moisturizing

Cream, Soaps,

Baby Products,

Eye Products(In

and Around The

Eyes)


Technique




(Conventional Plate count

method) Clause. 8.91,Chapter 8

≥ 3 MPN/g to

>1100 MPN/g

FDA- BAM: 2002

Chapter 4

≥3 MPN/g to

> 1100MPN/g


Coli




(MUG VRBA method)


≥10 cfu/g

Detection of Escherichia Coli IS 5887 (Part 1): 1976 (RA 2009) Qualitative






method) Clause. 8.73,Chapter 8

-X-X-X-X-X-X-X-X-X-X-X-X-X-X-

NOTE: The Laboratory has demonstrated competence for the stated scope for WATER. This however does not fully

cover the specification requirements of BIS for the Packaged Drinking Water as per IS:14543 and the Packaged

Natural Mineral Water IS:13428.

laboratory tuv sud south asia pvt. ltd., 373, udyog vihar ... · (conventional isolation method)...

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