Laboratory Safety Laboratory Safety RulesRules

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Appendix BAppendix B

In your textbookIn your textbook

Also in your Also in your

manualmanual……General Safety RulesGeneral Safety Rules

1. The laboratory is a place of serious work. 1. The laboratory is a place of serious work. Absolutely no practical jokes or misconductAbsolutely no practical jokes or misconduct will be tolerated will be tolerated

2.2. Safety goggles must be worn Safety goggles must be worn by everyone in the room during experiments involving caustic or corrosive chemicals, heat or flames, and by everyone in the room during experiments involving caustic or corrosive chemicals, heat or flames, and hot liquids or glassware.hot liquids or glassware.

3. Report any accident or injury no 3. Report any accident or injury no matter how minor, to your matter how minor, to your instructor instructor immediately.immediately.

4. 4. Prepare for each laboratory activity Prepare for each laboratory activity by by reading all instructions reading all instructions BEFOREBEFORE coming to class to coming to class to perform the experimentperform the experiment. Follow . Follow carefully any change in procedure carefully any change in procedure given by the instructor.given by the instructor.

5. Always follow written and verbal 5. Always follow written and verbal instructions given by instructor.instructions given by instructor.

6. Use 6. Use onlyonly materials and equipment materials and equipment authorized by the instructor. Perform authorized by the instructor. Perform onlyonly those laboratory activities where those laboratory activities where instructions and permission have instructions and permission have been given by your instructor.been given by your instructor.

7. If you have missed instruction on 7. If you have missed instruction on how to use certain equipment, ask how to use certain equipment, ask your instructor to demonstrate its your instructor to demonstrate its proper use before you proceed.proper use before you proceed.

8. Dispose of chemicals, broken glass, 8. Dispose of chemicals, broken glass, solid waste, and other materials solid waste, and other materials properly properly as instructed. as instructed. Only Only certain liquids should be washed certain liquids should be washed down the sinks since they have down the sinks since they have nono garbage disposals, and some garbage disposals, and some solutions are toxic to landfills. solutions are toxic to landfills.

Do not place matches, or broken Do not place matches, or broken glass into the sinks.glass into the sinks. Only Only liquids safe to go down the liquids safe to go down the draindrain can be placed there. can be placed there.

9. Always report broken materials, and 9. Always report broken materials, and damaged or improperly working damaged or improperly working equipment to the instructor equipment to the instructor immediatelyimmediately..

10. Never carry hot equipment or 10. Never carry hot equipment or dangerous chemicals through a group dangerous chemicals through a group of students.of students.

11. Never taste anything, or touch 11. Never taste anything, or touch chemicals with your hands unless chemicals with your hands unless specifically instructed to do so. Keep specifically instructed to do so. Keep hands away from your face, eyes, and hands away from your face, eyes, and body while using chemicals or body while using chemicals or solutions. If chemicals or solution do solutions. If chemicals or solution do come in contact with your skin, wash come in contact with your skin, wash the area immediately.the area immediately.

12. After performing any experiment, 12. After performing any experiment, always wash your hands before leaving always wash your hands before leaving the room.the room.

13. Eating or drinking in the laboratory 13. Eating or drinking in the laboratory area is area is notnot permitted at any time. permitted at any time.

14. During laboratory activities, keep long 14. During laboratory activities, keep long hair out of the way. Roll up long sleeves hair out of the way. Roll up long sleeves above the wrist. Excessive and bulky above the wrist. Excessive and bulky clothing, long-hanging necklaces, or clothing, long-hanging necklaces, or loose-fitting bracelets should not be loose-fitting bracelets should not be worn.worn.

15. 15. When removing an electrical plug When removing an electrical plug from the socket, pull the plug, not from the socket, pull the plug, not the electrical cord. Keep electrical the electrical cord. Keep electrical plugs away fro water, or other plugs away fro water, or other liquids.liquids.

16. Students are not permitted in the 16. Students are not permitted in the laboratory storage room without the laboratory storage room without the approval of the instructor.approval of the instructor.

17. Know the locations and proper use of 17. Know the locations and proper use of the emergency shower, eye, and face the emergency shower, eye, and face wash fountain, fire blanket, and fire wash fountain, fire blanket, and fire extinguishers. Know the proper fire drill extinguishers. Know the proper fire drill procedure.procedure.

18. When you have completed an 18. When you have completed an experiment, clean, dry, and return all experiment, clean, dry, and return all equipment and materials to its proper equipment and materials to its proper place as instructed.place as instructed.

19. Always keep your working area clean.19. Always keep your working area clean.

Chemical Safety RulesChemical Safety Rules

1. Never breathe fumes directly. Always 1. Never breathe fumes directly. Always test for odor of chemicals by waving test for odor of chemicals by waving your hand over the container and your hand over the container and sniffing cautiously from a distance.sniffing cautiously from a distance.

2. Never pour chemicals back into the 2. Never pour chemicals back into the stock container. Never exchange stock container. Never exchange stoppers, or lay them on the table.stoppers, or lay them on the table.

3. 3. When diluting an acid or base, always When diluting an acid or base, always pour the acid/base into the water, pour the acid/base into the water, never pour water into an acid/base.never pour water into an acid/base.

4. In case of a burn from an acid or a 4. In case of a burn from an acid or a base, wash the affected area base, wash the affected area immediately with plenty of running immediately with plenty of running water. If the eye is involved, wash it water. If the eye is involved, wash it continuously in the eye wash continuously in the eye wash fountain for at least 15 minutes. fountain for at least 15 minutes. Report the incident to your instructor Report the incident to your instructor immediately.immediately.

5. Check labels before taking 5. Check labels before taking chemicals or solutions. Make sure chemicals or solutions. Make sure equipment is being used in the equipment is being used in the proper manner. Report and proper manner. Report and malfunctions to the instructor malfunctions to the instructor immediately.immediately.

6. Never use mouth suction in filling 6. Never use mouth suction in filling pipettes. Always use a suction pipettes. Always use a suction bulb or pipette pump.bulb or pipette pump.

Heat, Fire, and Flame Safety Heat, Fire, and Flame Safety RulesRules

1. When lighting Bunsen burners, always 1. When lighting Bunsen burners, always light the match before turning on the gas.light the match before turning on the gas.

2. Never leave Bunsen burners or hot plates 2. Never leave Bunsen burners or hot plates that are in use unattended. Turn them that are in use unattended. Turn them off and unplug the hotplate when not in off and unplug the hotplate when not in use.use.

3. Never reach over a flame or hot plate. 3. Never reach over a flame or hot plate. Keep your head and clothing away from Keep your head and clothing away from flames and heat.flames and heat.

4.4. When heating test tubes, always tilt When heating test tubes, always tilt the tube, and never point it in the the tube, and never point it in the direction of other students. Never direction of other students. Never look down into the tube while look down into the tube while heating it.heating it.

5.5. Alcohols or other volatile material Alcohols or other volatile material that can easily burst into flame may that can easily burst into flame may not be used in the area of an open not be used in the area of an open flame.flame.

Lab MaterialsLab Materials

Triple beam balanceTriple beam balance

Electronic balanceElectronic balance

BeakerBeaker

FlaskFlask

Bunsen burnerBunsen burner

Petri dishPetri dish

Test tubes Test tubes

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test tube racktest tube rack

Test tube clamp/holderTest tube clamp/holder

Test tube brushTest tube brush

Hot plateHot plate

w/ flaskw/ flask

Graduated Graduated

cylindercylinder

Rubber stopperRubber stopper

PipettePipette

DropperDropper

ThermometerThermometer

Safety gogglesSafety goggles

MicroscopeMicroscope

That’s all folks!That’s all folks!