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Laboratory Laboratory Biohazardous/Sharps Biohazardous/Sharps Waste Management Waste Management

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Page 1: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Laboratory Laboratory Biohazardous/Sharps Biohazardous/Sharps Waste ManagementWaste Management

Page 2: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Types of Biohazardous Types of Biohazardous WasteWaste

Dry Solid- Dry Solid- No pourable liquids!No pourable liquids!– Contaminated Containers such as: Contaminated Containers such as:

Petri DishesPetri Dishes Conical TubesConical Tubes

– Contaminated Transfer DevicesContaminated Transfer Devices Pipette TipsPipette Tips Plastic PipettesPlastic Pipettes

Page 3: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Liquids – Pourable WastesLiquids – Pourable Wastes– StocksStocks– MediaMedia– BloodBlood– Aspirated Liquid WastesAspirated Liquid Wastes

Types of Biohazardous Types of Biohazardous WasteWaste

Page 4: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Sharps – Have the ability to cut or Sharps – Have the ability to cut or puncture.puncture.– Pasteur pipettes Pasteur pipettes – Syringes with needlesSyringes with needles– NeedlesNeedles– Razor BladesRazor Blades– Microscope slidesMicroscope slides

Types of Sharps WasteTypes of Sharps Waste

Page 5: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Biohazardous Waste Biohazardous Waste CollectionCollection

Dry Solids (No sharps!)Dry Solids (No sharps!)– Primary Containment:Primary Containment:

Collect dry, solid waste in Collect dry, solid waste in a “red bag”. The red bag a “red bag”. The red bag must have the must have the international biohazard international biohazard symbol, the word symbol, the word “biohazard” and a “biohazard” and a generator address label generator address label shown below (includes shown below (includes building & room number):building & room number):

San Diego State University5500 Campanile DriveSan Diego, CA 92182(619) 594-6778Bldg & Room Number: NLS-121 “Generator address

label”

Page 6: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Generator Address LabelsGenerator Address Labels

The Generator Address Labels include the following The Generator Address Labels include the following information:information:

Facility NameFacility Name Facility AddressFacility Address Phone NumberPhone Number Building and Room NumberBuilding and Room Number

-Laboratories on Campus can request these labels from the -Laboratories on Campus can request these labels from the EH&S DepartmentEH&S Department

-Off Campus facilities must generate their own labels -Off Campus facilities must generate their own labels which includes their specific informationwhich includes their specific information

Page 7: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Biohazardous Waste Biohazardous Waste CollectionCollection

Dry Solids (No sharps!)Dry Solids (No sharps!)– Secondary Secondary

Containment:Containment:

The red bag (primary The red bag (primary containment) must be containment) must be stored in a rigid container stored in a rigid container with a lid that is resistant with a lid that is resistant to leaks and punctures. to leaks and punctures. The red bag must be kept The red bag must be kept in the secondary container in the secondary container during use, storage, and during use, storage, and transport.transport.

Page 8: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Biohazardous Waste Biohazardous Waste CollectionCollection

Dry SolidsDry Solids– In the picture on the In the picture on the

right, the red bag is the right, the red bag is the primary container while primary container while the rigid can is secondary the rigid can is secondary container.container.

– The secondary container The secondary container must also have the must also have the international biohazard international biohazard symbol and the word: symbol and the word: “Biohazard”“Biohazard”

Red Bag

Page 9: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Acceptable and Unacceptable Acceptable and Unacceptable Secondary ContainersSecondary Containers

Not acceptable in CAMust be rigid, puncture-proof, leak-proof

Page 10: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Biohazardous Waste Biohazardous Waste CollectionCollection

Dry Solids– Labels containing the

international biohazard symbol and the word “Biohazard” have to be affixed on all 4 sides of the secondary container AND on the lid.

– For secondary containers that For secondary containers that are cylindrical, the Labels must are cylindrical, the Labels must be on three equidistant sides be on three equidistant sides AND the lid.AND the lid.

Page 11: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Biohazardous Waste Biohazardous Waste CollectionCollection

Biohazardous Liquid Waste Biohazardous Liquid Waste (Temporary Storage)(Temporary Storage)– Collect in a non-breakable container with Collect in a non-breakable container with

lid and labeled with the international lid and labeled with the international biohazard symbol and the word biohazard symbol and the word “Biohazard”.“Biohazard”.

– The container needs to be in secondary The container needs to be in secondary containment. containment.

Page 12: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Biohazardous Waste Biohazardous Waste DisposalDisposal

Biohazardous Liquid Waste DisposalBiohazardous Liquid Waste Disposal– Treat the liquid by disinfecting it with a Treat the liquid by disinfecting it with a

10% bleach solution.10% bleach solution.– Let the solution stand for 20 minutes.Let the solution stand for 20 minutes.– Discard down sink drain, then flush with Discard down sink drain, then flush with

water.water.

Page 13: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Sharps Waste CollectionSharps Waste Collection

SharpsSharps– Collect in a rigid puncture Collect in a rigid puncture

and leak resistant and leak resistant properly labeled properly labeled container.container. The words “Biohazardous”The words “Biohazardous” Must have International Must have International

Biological Hazard symbolBiological Hazard symbol Generator Address Label:Generator Address Label:

San Diego State University5500 Campanile DriveSan Diego, CA 92182(619) 594-6778Bldg. & Room #:_______

San Diego State University5500 Campanile DriveSan Diego, CA 92182(619) 594-5200Bldg.&Room#:________

Page 14: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Waste DisposalWaste Disposal Take biohazard bag and sharps container to Take biohazard bag and sharps container to

designated Accumulation Sites: designated Accumulation Sites: – Life Science/Bioscience Center Receiving Life Science/Bioscience Center Receiving

AreaArea– Calpulli Calpulli Center/Student Health Services Center/Student Health Services

Building on the East side Building on the East side – Generators at other locations on campus Generators at other locations on campus

may call EHS at (619) 594-6778 for may call EHS at (619) 594-6778 for biohazard waste pick-ups biohazard waste pick-ups

A biowaste vendor will collect biohazard bag A biowaste vendor will collect biohazard bag and sharps container for disposal on a and sharps container for disposal on a weekly basis from Life Science and Student weekly basis from Life Science and Student Health Services. Health Services.

Biohazardous and sharps wastes will be Biohazardous and sharps wastes will be autoclaved while animal carcasses will be autoclaved while animal carcasses will be incinerated.incinerated.

Page 15: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Common ProblemsCommon Problems

Pasteur pipette in red bag No Generator Label

Overfilled biological waste container and no lid

1.Don’t place sharps in red bags.

2.Always use “Generator Labels”

3.Don’t overfill bio containers.

1 2

3

Page 16: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Other ProblemsOther Problems

DO NOT use hazardous waste labels for DO NOT use hazardous waste labels for biohazardous waste.biohazardous waste.

Sharps containers must be removed seven Sharps containers must be removed seven days from when the lids are locked or tape days from when the lids are locked or tape sealed.sealed.

Red bags must be removed seven days Red bags must be removed seven days from initial use or more frequently if odor from initial use or more frequently if odor poses nuisance.poses nuisance.

Page 17: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Common ProblemsCommon ProblemsFull red bag on floor Full red bag on bench

Red bags must be in secondary containment at all times. Never place a full bag on the bench or the floor without proper secondary containment.

Page 18: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

What’s Wrong with these What’s Wrong with these Pictures?Pictures?

Left: Do not deface container. Incorrect label placed on container (need generator label).

Right: Red bag must be transported in a secure secondary container to the accumulation site. Red bag must have biohazard label and generator label.

Page 19: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Guidelines for Biohazardous Guidelines for Biohazardous WasteWaste

Refer to the Biohazard/Sharps waste Refer to the Biohazard/Sharps waste disposal guideline in SDSU’s EH&S disposal guideline in SDSU’s EH&S website for more detailed information:website for more detailed information:

– http://bfa.sdsu.edu/ehs/medbiodispos.htmhttp://bfa.sdsu.edu/ehs/medbiodispos.htm

Page 20: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Frequently Asked QuestionsFrequently Asked Questions Q: What do I do if my Red Bag has been Q: What do I do if my Red Bag has been

punctured?punctured?

A: The easiest thing to do is to place it into A: The easiest thing to do is to place it into another empty Red Bag then close and seal it and another empty Red Bag then close and seal it and do this until you are confident that it will not do this until you are confident that it will not puncture again. If this is a consistent problem, puncture again. If this is a consistent problem, you need to change your waste collection you need to change your waste collection procedure. For example, you can go to a thicker procedure. For example, you can go to a thicker gage of Red Bags, switch to a sharps container, gage of Red Bags, switch to a sharps container, or reduce the amount of material you place in a or reduce the amount of material you place in a Red Bag. Overfilling is one of most common Red Bag. Overfilling is one of most common source of punctures, leakage and expulsion of source of punctures, leakage and expulsion of contents.contents.

Page 21: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Frequently Asked QuestionsFrequently Asked Questions

Q: If my sharps waste are not contaminated Q: If my sharps waste are not contaminated with biohazardous material, do I have to with biohazardous material, do I have to dispose of them in a sharps container?dispose of them in a sharps container?

A: No, but it is the safest means to dispose A: No, but it is the safest means to dispose of sharps wastes for you and the people of sharps wastes for you and the people who empty your trash!! Whether your who empty your trash!! Whether your sharps wastes are contaminated or not, sharps wastes are contaminated or not, they need to be stored and disposed of in a they need to be stored and disposed of in a secure, rigid, puncture proof container.secure, rigid, puncture proof container.

Page 22: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Frequently Asked QuestionsFrequently Asked Questions

Q: Do I need to write down a start Q: Do I need to write down a start accumulation date on my Red Bag or accumulation date on my Red Bag or Sharps container?Sharps container?

A: A: NONO. Accumulation dates are only . Accumulation dates are only required on Hazardous Waste and required on Hazardous Waste and Universal Waste Universal Waste NOTNOT on Biohazardous or on Biohazardous or Sharps Waste.Sharps Waste.

Page 23: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Frequently Asked QuestionsFrequently Asked Questions

Q: If I have only a small amount of waste Q: If I have only a small amount of waste in my red bag and there is no strong odor, in my red bag and there is no strong odor, do I need to empty my Red Bag after 7 do I need to empty my Red Bag after 7 days?days?

A: Yes, it is a regulatory requirement. If A: Yes, it is a regulatory requirement. If you continually generate small amounts of you continually generate small amounts of biohazardous waste, we suggest that you biohazardous waste, we suggest that you try using smaller Red Bags and secondary try using smaller Red Bags and secondary containers for your waste.containers for your waste.

Page 24: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Frequently Asked QuestionsFrequently Asked Questions

Q: Does my Sharps Container or Q: Does my Sharps Container or Secondary Container need to be red?Secondary Container need to be red?

A: No, only Red Bags need to be “Red”. A: No, only Red Bags need to be “Red”. The other containers can be any color but The other containers can be any color but they do need the appropriate labeling.they do need the appropriate labeling.

Page 25: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

Frequently Asked QuestionsFrequently Asked Questions

A: My sharps container has a crack in it, A: My sharps container has a crack in it, what should I do?what should I do?

Q: You need to place it into a larger and Q: You need to place it into a larger and appropriately labeled container and then appropriately labeled container and then dispose of it. dispose of it. Do not attempt to Do not attempt to transfer contents from one container transfer contents from one container to another.to another.

Page 26: Laboratory Biohazardous/Sharps Waste Management. Types of Biohazardous Waste Dry Solid- No pourable liquids! Dry Solid- No pourable liquids! –Contaminated

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