king lab yearly safety review by cammie haase-pettingell for march 2005 disclaimer- this is jonathan...

King Lab Yearly Safety Review

by Cammie Haase-Pettingell for March 2005 Disclaimer-This is Jonathan King’s lab in Biology Dept. yearly safety review and not to be substituted for an initial

entry orientation. Any questions should be directed to Cammie 3-3545

March 19, 2004 CHP GM

Points be covered

Emergency Action Plan

Eating

Chemical

Radioactive

Biologicals, Blood Borne Pathogens, Infectious Agents

High Voltage


Responsibility

PI Ensure that the lab is conducting in a safe

manor This is by the PI or by a representative the

safety rep.Safety Rep. To conduct incoming safety orientation Go to safety meetings Update the lab on new policy

Individual To follow the above policy


Basics

No Eating in lab Can eat in

Tea room Computer room , hall and seminar room

No smoking except outsideNo mouth pipeting Story…


Evacuation Plan


Fire

Close the lab doors Pull the fire red fire alarm Located by Stairwells , Elevators

Leave by stairwellsGet to a phone- dial x100 report fire Don’t hang up till they have hung up

Not responsible to fight a fire


Unknown fire such as strange smell

If in doubt call x 100 Pull fire alarm

Other scenarioLook around Call John Fuccilo to ask he know anything Ex . melted stuff in autoclave


Evacuation

The equipment halls can’t hear the alarm As exit ensure people know about the alarm

If can - put experiments to rest- not required you safety is more important

So firefighters ie do not stumble upon a active electrophoresis.

Just in case not back for a while may not be ruinedEvacuate by stairwell -all are fire stairwellsMeet by the concrete post by bld 66

even if you think I saw you Check in so we know no one is stuck in the building

Then go off for what ever. Some one needs to go to the command post say we are all out

by the door toward Main St. -


Chemicals

MSDS see handoutChemical safety web training http://mitehs.traincaster.com/

Spill kits 68-340D behind door 68-322 under sink


Hoods

Simple Laminar Flow HoodEnsure the back “slot” in back is clear have items on lifts so it is not blocked

Simple test for flow to tape Kimwipe

If malfunctioning- inform fixit Zone -A http://web.mit.edu/facilities/www/Rooms air flow and temperature is dependant on the hood.


Spills

ChemicalRadioactivityBiological

General Clear Area Get on appropriate safety gear Determine the Scope Use the appropriated cleaner Dispose correctly

Look at the Red flip chart by phone


Chemical Spill

1. Evacuate area- put sign up2. Done appropriate safety - lab coat, eye

protection, appropriate gloves 3. Determine the necessary sorbant,

1. For acid base or solvent.4. Pour around the outside of the spill - mix in

Hold breath5. I use the dust pan and broom sweep up. 6. Wipe up residue7. Dispose of as waste


Chemicals and Medical Emergency

Do not block body showersEye wash put injured eye down flush for 15 min. Eyewash or distilled wash with tubing

Needs to be tempered waterCut and injury- should go to Medical

can be documented and treated properlySomeone collapses

Ensure you are not putting you self in danger If can rise -call EMT X100

EMT will be concern that they are not in danger try to figure what happened


Remember

Emergency number X100If working alone at night inform some one on the Hall Or call campus police 3-1212

Safety is important to everyone.


Chemical waste-

Waste needs a red tag Must be closed when not in use.Most is in electrophoresis room

Silver- silver stain MEOH-

Gel drying fix. , Transfer solution Ethidium bromide -

Liquid and tips Agarose in the dark room

Once full date and should be picked up immediately by law 2 days

If can call don’t date till wedded in link on King links page

http://web.mit.edu/environment/environmental/ehs_services/ehs_areas/hazardous_waste/forms/chem_collection.html


Sharps

Two types Biological or not chemical waste-gray bin

If it is sharp from a biological lab it can not be just thrown out.

Chemical waste- with red tags At the Bench Need to have covers and be covered when

not in use. Labeled


Radiation ext. 3-2180

We use 14C and 35S to label rapidly proteinsDisposal designated by 1/2 life

20-120day 1/2 life -35 S- 88 day 1/2 life Over 120 day 1/2 life 14C = 5730 yr 1/2 life

Type- dry (paper gloves), hi level aqueous, sharps/epi.

Radioactivity stocks is stored in the locked refrig and freezer 68-346D

Most experiment are preformed in the 68-346D.Geiger counters in each side of the lab.

Pancake probes more sensitive Ensure the battery is off when done.


Radioactive spill

Clear the area- block offEnsure they you have lab coat, safety glasses, double gloves, boots?Monitor the area with Geiger counter Mark the area- where there are countsOne does not inactivate the radioactive just clean off the solution.

Good agents -Bleach, Radiact wash, Am4OH Clean till no signal

RPO will do wipe test If still signal cover till decayed.


Biological

BL1- E. Colilow BL2 - Salmonella typhurminium LT2Clean up All bacteria needs to be in activated by

before disposal or going to the kitchen Autoclaving Chemical, 10% bleach, 1% Westcodyne,

LysolWaste Plates go into the bucket autoclaved in big

autoclave after kitchen hours.


Biological spills

Block the areaLet aerosol settleSafety gearClean up by pour/spray disinfectant on glassware with dusty pan

Put paper towel on it and pour/spray disinfectant on itDispose of in the waste.

Look for the new biological spill kits


Decontaminating the Bench

70% ethanol is a better agent to decontaminate the bench than 100% ethanol 70% is better at denaturing the outer

membrane and subsequently lysing the cell 100 % will only causes effects the on the

outer membrane but not lysis.


High Voltage

Any voltage should be treated as if it is dangerous. AC current is the most dangerous.

Running a PAGE Start up a gel by connecting the black lead first as a ground

and then the red lead. It should be noted that high power leads should be used

when running a sequencing gel with high voltage. Turn off a power supply by turning down the voltage source,

then turn off the power. After turning off power, disconnect the red (positive)

lead first.If the hazard light is flashing on the power supply, turn it off and determine what the problem is and fix it, or have it fixed.If someone is unconscious, call x100 which is the emergency phone number at MIT.


Chemical Hygiene Plan

OSHA right to know MSDS know how to readSOP- Standard Operating Procedures a written procedure for use of every

chemical


Safety is everyone responsibility

Everyone will be responsible for the level 1 check list which I email the pdf keep check off for a month Cammie and Shannon are the safety Reps to

do an over view ensure all unlabeled liquid containing item have a label can be cryptic

king lab yearly safety review by cammie haase-pettingell for march 2005 disclaimer- this is jonathan...

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