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James J. Salazar, Ph.D. Associate Professor of Biology Coordinator Life Sciences Program Galveston College 4015 Avenue Q Galveston, TX 77550 Tel.: 409 944-1278 Jsalazar@gc.edu CURRICULUM VITAE Fall 2014 HOME ADDRESS 3008 Bay Bend Ln Dickinson, TX 77539 Phone (281) 534-4927 EDUCATION University of Antioquia, Colombia, Ph.D. (molecular biology) Javeriana University, Colombia, Master (biology) Quindío University, Colombia, Bachelor in Education (major biology) NATIONALITY US American citizen PROFESSIONAL INTEREST, TEACHING EXPERIENCE AND APPOINTMENTS My main field of teaching is in general biology and biotechnology courses. My research expertise is in environmental biology and molecular toxicology using in vitro (cultured cells, bacteria) and in vivo (knockout mice) systems and molecular biology approaches. Key words of expertise and teaching interest: general biology, biotechnology, molecular biology, environmental biology/toxicology, genetic toxicology, biomonitoring, envirogenomics, mutagenesis, mutagenesis in complex mixture, chemical carcinogenesis, DNA damage and repair, oxidative stress, cells in culture, PCR, Ames test, 32P-postlabeling, Comet assay. I earned a degree of Doctor of Science (1997) from the Graduate School of Biomedical Science, field of molecular biology, at the University of Antioquia (UA) in Colombia. This was the first Doctoral degree granted by UA on a cooperative international exchange program in junction with The University of Texas Medical Branch (UTMB), Galveston, TX, USA. I worked with Dr. Bennett Van Houten (dissertation Advisor) in the Sealy Center for Molecular Science (SCMS), UTMB, in a research program on “ROS-induced oxidative DNA damage and repair”. While working in Dr. Van Houten’s laboratory, I accumulated experience in molecular mutagenesis using a quantitative long PCR system (QPCR) for the detection of DNA damage and repair in human cells. This is an important and novel approach for studying DNA damage and repair in specific genes.

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APPOINTMENTS August 2010-Present: Coordinator Life Sciences Program, Galveston College Responsibilities: Coordinate varies departmental activities, oversee budget, schedule courses, hire and supervise adjunct faculty and dual credit instructors. July 2004-Present: Associate Professor of Biology and Coordinator Biology Laboratory, Division of Academics, Galveston College Responsibilities: Teach both full lectures and laboratories of general biology for science majors I and II (BIOL1406, BIOL1407), Environmental Science I and II (ENVR1401, ENVR1402), Human Anatomy & Physiology I (BIOL2401), and biotechnology courses. Courses format: face-to-face, hybrid, and online. I also coordinate biology laboratory activities and oversee two lab assistants (students hired part time) who serve and assist faculty in setting up biology laboratory practices. July 2004-August 2007: Director of the Biotechnology Program, Galveston College-UTMB Responsibilities: Teach BITC courses (Introduction to biotechnology, Laboratory methods and techniques, and Molecular biology), coordinate varies program activities, oversees budget, schedule courses, hire and supervise adjunct faculty. Director Biotechnology Program: Biotechnology is a joint program between Galveston College and UTMB. The program uses teaching and laboratory resources as well funding from UTMB. I report to UTMB and interact with its administrative personnel, faculty and staff. As a result of these interactions, the biotechnology program at GC and the clinical laboratory science (CLS) program at UTMB have signed an articulation agreement (2+2). This agreement allows students completing the program to be eligible for early acceptance into the UTMB-CLS program towards completion of their bachelors of science degree awarded by the school of allied health sciences. Another important accomplishment resulting from this close interaction with UTMB is the Work School awards received by students in the biotechnology program. This award allows biotechnology students working full-time at UTMB to reduce the number of hours from 40 to 32 per week and also pays for tuition fees, instructional material and books. July 2004-Present: Adjunct Faculty, The University of Texas Medical Branch, Galveston, Department of Preventive Medicine & Community Health (PM&CH), Division Environmental Toxicology. Responsibilities: Co-director of Postdoctoral Teaching Training course. As per the office of postdoctoral affairs from UTMB request, I redesigned two courses and developed two syllabi designed to instruct and train postdoctoral fellows from varies departments and research areas from UTMB to conduct actual classroom teaching. The courses and syllabi, CTPS 6125 (classroom observation) and CTPS 6126 (classroom/laboratory experience), have been approved by UTMB’s postdoctoral curriculum committees and were launched in fall 2013. This is a joint educational effort signed by presidents from both UTMB and GC. As part of this program, I also developed and currently teach a summer lectures series entitled: The Biology of Diseases. Lectures designed for premedical school students at UTMB within the Enrichment Program lead by Dr. Lisa Cain. Additionally, I designed a biology general knowledge pretest and posttest for applicants to this program. October, 2000 – July 2004: Scientist and Director of the Automated Comet system in the Sealy Center for Environmental Health & Medicine (SCEH&M), UTMB. Responsibilities: Researcher and co-investigator in the ARCH Program (Advanced Research Cooperation in Environmental Health) between UTMB, Galveston and Southern University, LA. My role within the ARCH program, besides collaborating and interacting with other investigators and members, involved assessment of molecular and mechanistic aspects of mutagenesis, DNA damage and repair of 1, 3-butadiene (BD) and its metabolites, butadiene diepoxide (BDO2) and butadiene monoepoxide (BDO).

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September 1997 - September 2001: Associate Professor (tenured-track), Department of Biology, (School of Natural Sciences) UA, Colombia. Responsibilities: Teaching upper level courses in biology curriculum and graduate/MS level In addition to my teaching and academic assignments during my tenure at UA, I was able to expand and reinforce an existing environmental and molecular mutagenesis teaching program at the department of biology acting as coordinator of the mutagenesis laboratory. I also worked with faculty from UA and UTMB toward the development of an international educational & scientific exchange program between the two institutions. As part of this exchange program and during my tenure at UA, several faculties from UTMB and Berkeley National Laboratory, CA were invited to participate in workshops and educational activities. See below, under teaching and educational activities, a list of undergraduate and graduate courses taught while in UA. November 1990 – November 1996: Visiting Scientist, PM&CH, Division of Environmental Toxicology and Sealy Center for Molecular Science -UTMB. Responsibilities: working under the supervision of Dr. Lee Jane W Lu, I learned and accumulated experience in detection of DNA adducts by the 32P-postlabeling technique. The post labeling assay for detection of pregenomic lesions is useful for studying animal and human exposure to environmental chemicals, in order to understand the interaction of chemicals and genes and the role of such compounds in cancer development processes, and assessing risk of exposure to environmental and occupational chemicals. I participated in mechanistic studies of carcinogenesis (and transplacental carcinogenesis) of in vivo exposure of human, monkeys, mice and fish to environmental compounds such as 3-methylcholanthrene, 3-MC, benzo (a) pyrene, B(a)P, 2-amino-3-methylimidazo [4,5-f] quinoline, IQ, cigarette smoking and butadiene. I also worked in a research program on "quantitative and molecular analysis of specific-locus mutations resulting from gene-specific oxidative DNA damage and repair". I learned and gained experience working with the Chinese hamster ovary cells (CHO) system, a widely used in vitro technique utilized for studies of cytotoxicity (by cloning efficiency), clones isolation and mutational analysis in the hprt gene. Other research activities/positions: April 15 - May 12, 2000, Sealy Center for Molecular Cardiology (SCMC), UTMB. I Visited Dr. Scott W. Ballinger’s laboratory to conduct experiments on mitochondrial DNA damage as response to environmental toxicants. July 27 - August 30, 1997, Sealy Center for Molecular Science (SCMS). I visited Dr. Bennett Van Houten’s laboratory to conduct and complete experiments on mitochondrial response to ROS exposure. 1986 -1989, Researcher Assistant, UA, Department of Biology, participated in environmental mutagenesis studies using in vitro (Ames test) and in vivo (mice micronucleus) assays. Special emphasis in mutagenesis of herbicides paraquat and glyphosate. I also participated in preliminary studies related to separation, characterization and genotoxicity of mutagens formed in cooked foods. 1982-1983, Researcher Assistant, Javeriana University (Ad hoc), graduate school of biological science, Bogota, Colombia. I helped and assisted course director of graduate level courses in cytogenetics and cell culture and in vitro techniques. TEACHING AND EDUCATIONAL ACTIVITIES My teaching assignments with GC started in spring 2004 when I joined the biotechnology program as an adjunct faculty to teach evening courses (Introduction to Biotechnology-BITC1311 and Laboratory Methods and Techniques-BITC1402). In July 2004, I was appointed as a full-time tenure-track associate professor to serve biotechnology and biology courses. I currently teach both lectures and laboratories of general biology for science majors I and II (BIOL 1406, BIOL1407), Environmental Science I and II (ENVR1401, ENVR1402), and Human Anatomy & Physiology I (BIOL 2401) courses within the division of academics at Galveston College.

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As a need for non-science major students at GC, I have developed and taught two environmental science courses face-to-face (ENVR1401 and ENVR1402). Additionally, starting fall 2013, I designed (and teach) a fully online version of these 2 courses. I have also taught the following courses in the biotechnology program: Laboratory Methods & Techniques (BITC 1402), Molecular Biology Techniques (BITC 2401), Laboratory Instrumentation (BITC 1401), and a portion of Introduction to Biotechnology (BITC 1311). My teaching and academic responsibilities in the department of biology, UA (September 1997-September 2001), included teaching in biology courses, mentoring students, coordinating an environmental and molecular mutagenesis teaching laboratory where upper level undergraduate students and faculty received training covering the basics of molecular biology techniques and short term toxicology assays. While at UA, I taught the following upper level undergraduate and graduate level courses: UNDERGRADUATE: Introduction to research in mutagenesis (4-credit lecture and laboratory) Seminar: introduction to mutagenesis GRADUATE (MASTER LEVEL): Genetic toxicology (3 credit-lecture-theoretical) Seminar: topics in genetic toxicology JOURNAL CLUBS (noncredit but biology upper level students majoring in genetics were expected to participate) Topics on oxidative DNA damage and repair Environmental impact: mutagenesis in complex mixtures, air, water and foods AWARDS AND RECOGNITION May 2012 NISOD - Excellence Award (NISOD: The National Institute for Staff and Organizational Development, a consortium of over 700 community colleges and universities worldwide) 2011-2012 Galveston College - Faculty Exceptional Service Award February 2012 TACHE – Distinguished Community College Faculty Award (State-wide recognition from the Texas Association of Chicanos in Higher Education) June 2010 NISOD - Excellence Award (NISOD: The National Institute for Staff and Organizational Development, a consortium of over 700 community colleges and universities worldwide) 2009-2010 Galveston College - Faculty Exceptional Service Award September 1996 - International program award for excellence in research, Sealy Center for Molecular Science - SCMS, UTMB, Galveston, TX September 1995. Scholarship award to complete doctoral program. Colombian Institute for the Development of Science “Francisco Jose de Caldas”, Colciencias, 3-year coverage from September 1, 1995 - August 31, 1998. Scholarship financed by Colombian government, with funds of Colciencias-BID loan, III stage November 1994. Travel award as an invited graduate student to international course: "genes involved in DNA repair and molecular techniques for its study", Nov. 1994, Colombian Institute for the Development of Science “Francisco Jose de Caldas”, Colciencias - University of Antioquia, Medellin, Colombia

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November 1992. Travel award to attend DNA damage workshop: "DNA adducts detection using the post labeling technique". Colombian Institute for the Development of Science “Francisco Jose de Caldas”, Colciencias. University of Antioquia. November 3-11, 1992 INTERNAL AND EXTERNAL SERVING COMMITTEES Fall 2013-present Galveston College Area Safety Liaison (ASL) Fall 2012 - present Galveston College President’s Council Member / Full-time Faculty Representative Spring 2012- present Tenure, Rank, and Sabbatical Committee (Appointed Chair starting fall 2013 – present) Fall 2009-present Galveston College Scholarship and Financial Aid Committee (Appointed Chair – 2010-2011, 2013-2014 and 2014-2015) Fall 2010 – Fall 2011 and 2013-2014 Galveston College Student Service Council Fall 2013-2014 Galveston College Instructional Affairs Council Fall 2006-Fall 2009 Galveston College Faculty Professional Development Committee Fall 2004-Fall 2006 Galveston College Recruitment and Retention Committee Spring 2009-present Galveston College Developmental Education Taskforce Fall 2004-present Galveston County Science & Engineering Board member (Co-chair) Chair: years 2009 and 2010 Fall 2009-present Member of the Biotechnology Advisory Council for Clear Creek Independent School District (CCISD) March 2006-present Member of the Institutional Animal care and Use Committee (IACUC) of NeuroBioTex, Inc Galveston, TX September 1997-2000 Biosafety Committee, Department of biology, UA January 1998 – September 2000 International Affairs Delegate, UA February 22-25, 2000 U. Cauca, Colombia, IV Colombian meeting in genetics (member of organizing committee)

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November 24-27, 1998 UA, School of Medicine, III Colombian meeting in genetics (member of organizing committee) MENTORING/SUPERVISING STUDENTS and Faculty Spring 2015 - mentoring Dr. Fangian Guo, postdoctoral fellows from UTMB registered in Teaching Training Program CTPS6125 (classroom observation) Spring 2015 - mentoring Dr. Carrie McAllister and Kimberly Roberts Postdoctoral fellows from UTMB registered in Teaching Training Program (fall 2014 took CTPS6125 (classroom observation) and CTPS6126 (practicum) current spring semester. Fall 2013 - mentoring Dr. Courtney Cross and Dr. Calvin Henard, Postdoctoral fellows from UTMB registered in Teaching Training Program and currently taking CTPS6125 (classroom observation) and CTPS6126 (practicum). Mentoring Dr. Srirajya Rudrabhatla, Biology Intern, from the California State University during the fall of 2010 as part of her Community College Teaching Training Program. Mentoring Program at GC: I signed up and participate (2005-2006) in a Mentoring Program at GC. This program matches students with faculty who signs on volunteer basis. I enjoy discussing and helping students in the designing of their career path and developing all their potential. Unfortunately this program has been inactive. As a result of my mentoring interest and activities, I have had the privilege to work with a number of students throughout my academic appointment. I have listed some of the students I have worked with in the past few years: Patrick Newman and Robin Demack, Lucero Aranda, Isabela Flores and Patrick LaCrosse (Biotechnology program), supervising Internships I and II during summer sessions 2004 and 2005 Jade Truong, Alex Torres, Tina Brown and Venkata-Usha Mokkapati (Biotechnology program), supervising Internship I (summer 2006) and Internship II (spring 2007). Oscar E. Masters (University of Oklahoma), summer 2003, participant of Summer Undergraduate Research Program -SURP- UTMB. Working under my mentorship in SURP, Oscar was a recipient of two awards: a) The Robert A. Welch Foundation Award in Chemistry for Outstanding Biochemical Research and, b) Toxicology Scholarship (Run Up). Elizabeth Silva, National Institute of Health (NIH-Colombia): Provided advanced training in Ames test and molecular aspects of environmental mutagenesis. Mentoring UA – undergraduate and graduate students’ dissertations/ theses: Lina M. Ruiz, Nelly Velázquez, Juan F. Correa, Luis C. Naranjo, Ivan Meléndez, Luz Y. Orozco, Elizabeth Rincón OUTREACH-EDUCATIONAL ACTIVITIES As an effort to attract students to Galveston College (specifically to the biotechnology program), I interact with school’s administrators, faculty and staff from GISD and surrounding areas. I visit middle and high school campuses to give seminars and to introduce the program to students and to excite them about science and the educational and training opportunities that the program has to offer. I have designed a presentation entitled: Biotechnology Program: Career Planning and Opportunities. Some of the visited schools include: Ball HS, Austin MS, O’Connell HS in Galveston, and Dunbar MS in Dickinson. The direction of the Outreach Program and its long term goal is to design an Introduction to Biotechnology course to be offered in middle and high schools at GISD. This will help to seed the biotechnology culture in the area.

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I have also designed a workshop hands-on training activity entitled: DNA Science: an introduction to biotechnology to be presented at middle and high schools. This workshop consists of two sessions: a lecture session about DNA science covering history and basic structure and function of DNA, and a laboratory session where students have the opportunity to isolate DNA from their own cells. I have participated in KIDS’ COLLEGE and MATH & SCIENCE YOUTH ACADEMY activities within Galveston College Continuing Education summer programs (Summer 2005 and scheduled for summer 2006). I volunteer to participate in these programs by giving lectures, seminars and laboratory practices to middle and high school participants. As part of the recruitment effort, I also participate in COLLEGE NIGHT activities scheduled by Galveston College. I have participated, as a Judge or mentor, in a number of educational events such as: Galveston County Science & Engineering Fair (several years), Dickinson Independent School District (DISD- several years), TAMUG Student Research Symposium (April 21, 2010), Galveston Early College High

School (GECHS – several years), UTMB Summer Undergraduate Research Programs – SURP (bench tutorials - years 2000-20030, and 2012 STEM Education Conference. I participated in the Health Occupation Student Association (HOSA) Leadership Conference (April 12, 2013). I participated as judge for two of the skills assessment within the biotechnology field competition (Gran stain and Using a microscope). During the spring of 2011, I volunteered in a project led by the National Institute of Health division of the Human Genome Project (HGP) and intended to review and to translate (English-Spanish) some portions of the HGP. The translations are available to teachers, students, and general public interested in this field. Portions of my contributions include the review and translation of “The Glossary of Terms” and “Test Your Gene IQ". The latest includes quick movie interactive files. In spring 2013, in junction with Dr. Darrell Carney (Department of Biochemistry UTMB), I developed a new Teaching Training course designed to train postdoctoral fellows from UTMB who are interested in acquiring teaching skills and models (CTPS 6125 and CTPS 6126). These syllabus/courses passed through rigorous evaluations from GSBS curriculum and executive committees at UTMB before they could be open and available to postdoctoral instructors.

I also serve as frequent chapter/book reviewer for well-known publishers such as Pearson. I reviewed several chapters (9, 15, and 16) for the first-edition of “The Environment and You” by Dr. Christensen (spring 2012) and the second edition (spring 2013). INVITED SPEAKER / PROFESSOR (MEETINGS, WORKSHOPS, SEMINARS AND LECTURES) June 14, 2014, guest speaker at UTMB (The University of Texas Medical Branch) – Career Forum Sponsored by the Graduate School of Biomedical Sciences / Track 3: Academia / Teaching “Translational Teaching: from the bench to the classroom”. October 4, 2012, guest speaker at NASA - Johnson Space Center – Hispanic Heritage Month event “A Piece of my American Dream”. I received an Honor Certificate of appreciation from NASA. March 24, 2006, guest speaker at Galveston County Career & Technology Consortium meeting “Biotechnology program: Career Planning and Opportunities” February 16-20, 2004, Keynote speaker at the III international meeting in genetics and VI Colombian meeting in genetics, mutagenesis session. “Wild type and knockout mice used as a model of sensitivity in ecogenomics. 1, 3- Butadiene and its metabolites as a case study”. University of Norte, Barranquilla, Colombia.

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June 9, 2000, What is new in aging. "Molecular aspects of oxidative damage and aging". School of Medicine, UA February 22-25, 2000, Alexander Hollaender Workshop –“Environment and genetic toxicology” IV Colombian meeting in genetics. “Mutagenesis and genotoxicity of water extracts and chlorination byproducts” and “DNA adducts and its use in environmental biomonitoring”. Meeting conference: “genetic toxicology of reactive oxygen species”, U. Cauca, Colombia May 27 1999, European week. Meeting for an euro-colombian dialog. Organized by embassies of European Union in Colombia and RCI - “Ecogenotoxicology”. U. Medellin, Colombia May 14 1999, III Colombian meeting in genetics Workshop genetic susceptibility and health risk due to exposure to genotoxins. “Mitochondrial DNA susceptibility to oxidative stress and its ability to repair”. U. Cauca, Colombia November 24-27, 1998, “Mitochondrial and genomic instability caused by reactive oxygen species in human cells” School of Medicine, UA August 1997, “glucose oxidase, a model for chronic oxidative stress, induces extensive and persistent mitochondrial DNA damage in human cells”, The University of Texas Medical Branch, SCMS, NIEHS Center and toxicology training grant seminar Galveston, TX November 3-11, 1992, DNA damage workshop: "DNA adducts detection using the post labeling technique", UA April 1990, II Theory-practice workshop "detection of environmental mutagens using in vitro techniques. Ames test", UA September 1990, II Meeting biologist alumni “environmental mutagenesis. A general overview” U. Quindío, Colombia PROFESSIONAL DEVELOPMENT WORKSHOPS/CONFERENCES/MEETINGS ATTENDED Fall 2014 (September 29-November 7) – Participated in Faculty Reading Apprenticeship 101: A 6-week online course designed to help and mentor students to become better readers and to read appropriately for learning purposes. This course is designed for Community College faculty and directed by WestEd University. 28th Annual Conference – HAPS (Human Anatomy & Physiology Society) – Jacksonville, FL May 24-29, 2014 – Hyatt Hotel and South Florida Community College On Course I – workshop: Innovate strategies for empowering students to become active, responsible learners. October 23-25, 2013 – Galveston College Science Faculty Workshop-presented by UHD and designed to discuss Texas College and Career Readiness Standards. April 26, 2013 - Moody Gardens, Galveston, TX 66th TCCTA Annual Convention – Houston, TX February 21-23, 2013 – The Westin Galleria Hotel STEMTECH Conference – Kansas City, Missouri October 28 – October 31, 2012 – The Sheraton/Westin Conference Center 2012 National Association of Biology Teachers (NABT) Professional Development Conference November 1 – November 3, 2012 – Hyatt Hotel at Dallas, TX 34th (NISOD) Annual International Conference on Teaching & Leadership Excellence – Austin, TX

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May 27 – May 30, 2012 – Austin Convention Center 12th Annual Texas A&M Assessment Conference – College Station, TX February 19-21, 2012 Galveston College Angel 7.4 Online Training Course April 26-May 13, 2011 (Full-Time Faculty using Angel) Student Advisement Training In-Service (Use of Colleague in Student Advisement) Galveston College – Friday April 15, 2011 (Full-Time Faculty and Program Coordinators) STEMTECH Conference – Orlando, Fl October 31 – November 3, 2010 – Walt Disney World Swan and Dolphin Resort 31st Annual Conference for Faculty Leaders – TCCTA - Galveston, TX October 8-9, 2010 – San Luis Resort 32nd (NISOD) Annual International Conference on Teaching & Leadership Excellence – Austin, TX May 30 – June 2, 2010 – Austin Convention Center 63rd TCCTA Annual Convention – Houston, TX March 4-6, 2010 – The Westin Galleria Oaks Hotel 2009 National Association of Biology Teachers (NABT) Professional Development Conference November 12 – 14, 2009 – Sheraton Hotel at Denver, CO - Invited to participated in special discussion panel on Introductory Biology Project (IBP) Transforming Teaching Through Learning (TTTL) seminar series February – April 2009 - Galveston College 62nd TTCTA annual convention - Austin, TX February 19-21, 2009 - Renaissance Austin Hotel Enhancing WebCT Tools Workshop November 14, 2008 – Galveston College 61st TTCTA annual convention – Dallas, TX February 21-23, 2008 - The Fairmont Dallas Hotel Science Education Workshop for Life Science and Chemistry February 1, 2008- The Houston Museum of Natural Science and Pearson Co. Building Community at Galveston College A five-session series meeting monthly from January – May 2008 Galveston College WECM Training session September 14, 2007 - Galveston College Emotional Intelligence – Webinar November 28, 2007 – VADER Room – Galveston College Student Advisement Workshop June 25, 2007 - Galveston College

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Developing Good Decision Making Skills A six-session series meeting monthly from December, 2006-May 2007 Galveston College Texas Skill Standards Board (TSSB) meeting March 31, 2006 - Austin Community College - Austin, TX Farfel Distinguished Lecture: “My Life with DNA” Doctor James D. Watson April 7, 2005 - University of Houston – Cullen Performance Hall The 19th Annual UTMB Undergraduate Research Symposium UTMB-Graduate School of Biomedical Sciences March 3-4, 2005 - UTMB Bioastronautics Technology Forum NASA – UTMB March 16, 2005 – UTMB ED101: Workforce Issues Galveston Economic Development Partnership and Galveston College May 6, 2005 - Galveston College Alliance for Workforce Development in Biotechnology for the Greater Houston Area April 4, 2005 – Greater Houston Partnership Headquarters First South Central Regional Biotechnology Programs meeting The following hands-on practices were performed as part of the meeting: Crime scene investigator (CSI), PCR basics; Genetic modified organisms (GMOs) and ELISA immuno explorer. NHCC-The Woodlands, Houston, TX NanoHealth Consortium Workshop February 2005 - Houston, TX Environmental Sentinels – NASA-JSC: a two-day technology-focused event on environmental monitoring and control technologies June 2004, Houston, TX Biotechnology Industry Organization (BIO) Conference and Workshops: Teacher Leader Program-Biotechnology Institute and the Sixth Annual Summer Fellows Forum, Bio-Link July 3-10, 2004 - San Francisco, CA Bio-Link Protein Purification Workshop: A five-day hands-on laboratory course. Supported by the National Science Foundation, August 2004, University of Austin, Austin, TX “How to write for publishing” - workshop August 1999 - UA, Colombia. “Induction to pedagogy” - workshop July 1999 - UA, Colombia Grants writing workshop March 1994, Sponsored by UTMB, Office of International Affairs

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Galveston, TX Molecular biology methods course This is a special training-course designed for researchers and graduate students, UTMB, Galveston, TX 1992 Basic radiation safety in the lab UTMB, Galveston, TX 1990 Application and special techniques in cells culture July 1983 - Javeriana University, Colombia Introduction to animal cells culture July 1982 - Javeriana University, Colombia ORGANIZING AND COORDINATING EDUCATIONAL & SCIENTIFIC EVENTS Oxidative DNA damage workshop “molecular biology of oxidative DNA damage and repair”. Invited professors: Dr. Bennett Van Houten, Ph.D. The University of Texas Medical Branch, Galveston, TX, USA and Dr. Priscilla K. Cooper, Ph.D. University of California, Berkeley, CA, USA UA, August 17-21, 1998 Miniseminar series: “measurement of DNA damage and repair” - Measuring gene-specific DNA repair capacity in mammalian cells using quantitative pcr - Reactive oxygen, mitochondrial damage and models of aging Invited Professor: Dr. Bennett Van Houten, Ph.D. The University of Texas Medical Branch, Galveston, TX, USA UA, September 1-3, 1997 DNA damage and repair workshop: "genes involved in DNA repair and molecular techniques for its study" Invited Professor: Dr. Bennett Van Houten, Ph.D. The University of Texas Medical Branch, Galveston, TX, USA UA, November 1994 DNA damage workshop: "DNA adducts detection using the post labeling technique" Invited Professor: Dr. Lee Jane W. Lu, Ph.D. The University of Texas Medical Branch, Galveston, TX, USA UA, November 3-11, 1992

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BIBLIOGRAPHY Full peer reviewed papers: A model of sensitivity: 1,3-butadiene increases mutant frequencies and genomic damage in mice lacking microsomal epoxide hydrolase activity. Jeffrey K. Wickliffe, Marinel M. Ammenheuser, James J. Salazar , Sherif Z. Abdel-Rahman, Darlene A. Hastings-Smith, Edward M. Postlethwait, R. Stephen Lloyd, and Jonathan B. Ward Jr., Environmental and Molecular Mutagenesis, vol 42 #2, 2003. Dexamethasone inhibits apoptosis of human neutrophils induced by reactive oxygen species. Lina M. Ruiz, Gabriel Bedoya, James J. Salazar, Diana Garcia, and Pablo Patino. Inflammation, Vol 26, No. 5, October 2002. DNA adducts of 2-amino-3-methylimidazo [4,5-f] quinoline (IQ) in fetal tissues of patas monkeys after transplacental exposure. Shylaja Josyula, Lee-Jane W. Lu, James J. Salazar, Pratibha V. Nerurkar, Ann Butler Jones, James J. Grady, Elizabeth Snyderwine, and Lucy M. Anderson. Toxicol. Appl. Pharmacol. 166, 151-160, 2000 A minireview: oxidative damage. The mitochondria as a target James J. Salazar Act. Biol., vol.22 #72, pp 103-115, 2000 Reactive oxygen species-induced mutants and toxicity in Chinese hamster ovary cells. James J. Salazar and Abraham W. Hsie Act. Biol., vol. 21 #70, pp 37-45, 1999 Preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide in human fibroblasts. James J. Salazar and Bennett Van Houten. Mutation Research/DNA Repair, 385:139-149, 1997 Absence of formation of benzo[a]pyrene/DNA adducts in the cuttlefish (Sepia officinalis, Molusca: Cephalopoda). Phillip G. Lee, Lee-Jane W. Lu, James J. Salazar, and Viktor Holoubek. Environmental and Molecular Mutagenesis, 23:70-73, 1994 Persistence, gestation stage-dependent formation, and interrelationship of benzo(a)pyrene-induced DNA adducts in mothers, placentae and fetuses of Erithrocebus patas monkeys. Lee-Jane W. Lu, Lucy M. Anderson, Ann B. Jones, Thomas J. Moskal, Sabine Rehm, James J. Salazar, James J. Hokanson and Jerry M. Rice. Carcinogenesis, 14: 1805-1813, 1993 Mutagenicity of five herbicides in Salmonella typhimurium. Zuleta, M., James J. Salazar and Adelaida G., Rev. Latinoam. Genet., 1: 321-328, 1990. Others: full papers published in peer review journals in Spanish Submitted: Mutagenicity and genotoxicity of extracts from consumption water and chlorination byproducts. Nelly Velásquez, Margarita Zuleta, Jairo Quijano and James J. Salazar Efecto de la dexametasona sobre la apoptosis de neutrofilos humanos inducida por especies reactivas de oxigeno. Lina M.Ruiz, James J. Salazar, Gabriel Bedoya, Diana Garcia de O., Pablo Patiño. Revista de Inmunoalergia [Revista de la Asociacion Colombiana de Alergia, Asma, e Inmunología], vol. 8 #4, pp. 19-25, Dic. 1999. Efecto Mutagenico de los herbicidas paraquat and glyfosato in Salmonella typhimurium. James J. Salazar and Margarita Zuleta, Act. Biol. vol. 6 No 60, pp 44-46, April-June, 1987.

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Abstracts presented / published and meetings attendance April 15, 2004. Annual Meeting of the Texas Genetics Society - TGS, are butadiene diepoxide DNA adducts recognized by nucleotide excision repair: an in vivo test using XPC knockout mice. JK. Wickliffe, J. Xie, LA Galbert, MM. Ammenheuser, JJ Salazar, JR. Carmical, RS. Lloyd, JB. Ward, Corpus Christi, TX March 25, 2004. Society of Toxicology Annual Meeting - SOT, A model of sensitivity: 1, 3-butadiene induces Hprt mutants in mice lacking microsomal epoxide hydrolase activity. Jeffrey K. Wickliffe, James J. Salazar, Marinel M. Ammenheuser, Lori A. Galbert, Jonathan B. Ward, Baltimore, MD October 17-18, 2003. Gulf Coast Society of Toxicology - GCSOT, Genomic damage in microsomal epoxide hydrolase knockout mice following a subaccute exposure of butadiene diepoxide. Masters, O, Salazar, J, Wickliffe, J, Ward, J., UTMB-Galveston, TX October 17-18, 2003. Gulf Coast Society of Toxicology - GCSOT, A model of sensitivity: 1, 3-butadiene induces Hprt mutants in mice lacking microsomal epoxide hydrolase activity. Jeffrey K. Wickliffe, James J. Salazar, Marinel M. Ammenheuser, Lori A. Galbert and Jonathan B. Ward, UTMB-Galveston, TX May 30, 2003. SCMS, annual science forum, UTMB, Galveston, TX May 10-14, 2003. 34th annual Environmental Mutagen Society - EMS meeting. Butadiene diepoxide causes differential DNA damage in lymphocytes from wild type and epoxide hydrolase knockout mice. Salazar JJ, Ward JB, Wickliffe JK and Lloyd RS. Fontainebleau Hilton Resort, Miami Beach, FL April 27-May 2, 2002. 33rd annual Environmental Mutagen Society - EMS meeting. 1, 3-butadiene and its metabolites-induced DNA damage in human and mouse cells. Salazar JJ, Ballinger SW, Postlethwait EM, Albrecht TB, Ward JB and Lloyd RS. Hilton Hotel, Anchorage, Alaska February 22-25, 2000. IV Colombian meeting in genetics. U. Cauca, Colombia November 1998. Mutagenic evaluation of water for human consumption in a Colombian population. (Evaluación mutagénica de aguas para consumo humano en poblaciones Colombianas). James J. Salazar et al., Abstract in Spanish. Genetics and Molecular Biology, Supplement, Vol. 21, #3, p.145 November 24-27, 1998. III Colombian meeting in genetics. University of Antioquia, School of Medicine. Mutagenicity of human consumption water treated in the Villa Hermosa plant. Influence of chlorine. Medellín, Colombia November 15-20, 1998. V Latin American meeting on environmental mutagenesis, carcinogenesis and teratogenesis: “preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide in human fibroblasts” and “mutagenic evaluation of human consumption water in Colombian population”. V Alexander Hollaender training course in genetic toxicology: “Detailing the methodologies of short term genetic toxicology assays”. Hotel Bourbon, Curitiba (Brazil) October 21-November 2, 1998. Graduate International Workshop: “protein separation techniques and enzymatic and non enzymatic modification of proteins”. University of Antioquia, Medellin, Colombia June 1997. I Minisimpossium, Corporacion de Ciencias Basicas Biomedicas, Universidad de Antioquia: “projects and progress in Basic Biomedical Sciences Research”, graduate students, “preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide in human fibroblasts”. Presentation of results, Medellin, Colombia November 1996. III Annual meeting of the oxygen society: “QPCR reveals reactive oxygen species induce preferential and persistent mitochondrial DNA damage in human and mouse cells”. Miami, FL

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May 1996. Science forum sponsored by Beckman Instruments, Inc. and SCMS. “Preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide”. Presentation of preliminary results, UTMB, Galveston, TX March 1994. Differential effect of maternal and fetal Ah genotypes on 3-methylcholanthrene (MC)-induced DNA damage in mothers, fetuses and placentae. Lee J. Lu, James J. Salazar and Lucy M. Anderson. The Toxicologist, 14:330 March 1994. 33rd. Annual meeting society of toxicology - SOT: "differential effect of maternal and fetal Ah genotypes on 3-methylcholanthrene-induced DNA damage in mothers, fetuses and placenta". Dallas, TX 1993, American Association for Cancer Research - AACR meeting. Food mutagen-DNA adducts in fetal non-human primates from 2-amino-3-methylimidazo[4,5-f]quinoline. Lee-Jane W. Lu, Nerurkar, P.V., James J. Salazar, Lucy M. Anderson, Jones, A.B., Elizabeth, G. Snyderwine, Thorgeirsson, S.S., and Rice J.M. Proc Amer. Assoc. Cancer Res. 34: 161 May 1993. 84th. American Association Cancer Research (AACR) meeting: "influence of maternal and fetal genotypes on 3-methylchlolanthrene-induced DNA damage in maternal and fetal livers". Lee-Jane W. Lu, James J. Salazar and Lucy M. Anderson. Proc. Amer. Assoc. Cancer Res. 34: 161, Orlando, FL Fall meeting1993, Gulf Coast Society of Toxicology – GCSOT, UTMB, Galveston, TX May 1992, 83rd American Association for Cancer Research - AACR meeting. Gestation stage-dependent formation and persistence of 3-methylcholanthrene-induced DNA adducts in maternal liver. James J. Salazar and Lee-Jane W. Lu. Proc. Amer. Assoc. Cancer Res. 33: 148, San Diego, CA 1992, American Association for Cancer Research - AACR meeting. Differential formation and persistence of 3-Methylcholanthrene-induced DNA adducts in bone marrow during gestation. James J. Salazar and Lee-Jane W. Lu. Proc. Amer. Assoc. Cancer Res. 33: 105 1992. IX Texas carcinogenesis meeting. Nutritional and genetics approaches to cancer prevention. The University of Texas MD Anderson Cancer Center. Houston, TX 1991. 82nd. AACR, meeting, Houston, TX Others: IV International meeting on tumor biology XVI COMALFI seminar Pesticides risk and their prevention XVIII National meeting of biological sciences V Latin-American meeting of electronic microscopic VII Latin-American meeting and I Colombian meeting of genetics XXI National meeting of biological science: "mutagenic effect of herbicides paraquat and glyphosate in Salmonella typhimurium". University of Valle, Colombia Methods workshop: March 13-17, 1994. Embryonic stem cells and gene knockouts. 84th. annual meeting AACR, Orlando, FL Strategies for cloning toxicant-inducible genes. SOT annual meeting, Dallas, TX In vivo and in vitro models of cellular transformation. 83rd. Annual meeting AACR, San Diego, CA

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RESEARCH GROUPS AND GRANT SUPPORT I have participated, as biotechnology program director, in collaborative grant writing efforts with other community colleges and universities in the Houston-Galveston area to attract funding to better equip our laboratories and as part of our educational and workforce training mission. In late summer 2005, a grant proposal “Houston Area Biotechnology and Life Sciences Training Education Partnership” was submitted to Department of Labor (DOL). Six Houston area community colleges participated in this effort: Galveston College, San Jacinto College, College of the Mainland, Houston Community College, Lee College and Montgomery College. Although the proposal was not funded, participants have agreed to meet and revise the grant to resubmit it. In late October of 2005, a joint proposal between the Alliance for Life Science Technology (ALiST) - headquarters in Houston Technology Center (University of Houston) - and several community colleges submitted a grant proposal “Bridge to the Future”. The grant was sent to National Science Foundation (NSF) and the goal of this grant is to develop curriculum and instructional materials for courses in the life sciences and biotechnology field. A Galveston College specific responsibility within the proposal is to plan and develop on line courses. I served as co investigator in an ARCH (Advanced Research Cooperation in Environmental Health) program between UTMB, Galveston and Southern university, LA. (October 12, 2000- July 12, 2004). The ARCH program involved evaluation of molecular and mechanistic aspects of mutagenesis, DNA damage and repair of 1, 3-butadiene (BD) and its metabolites, butadiene diepoxide (BDO2) and butadiene monoepoxide (BDO). Cells in culture and knockout mice are being used to help to answer some of the most relevant questions within the Program. Dr. Stephen Lloyd was the principal investigator and creator of the ARCH program. During my tenure at the Department of biology, UA, I served as the coordinator of environmental and molecular mutagenesis laboratory. Besides teaching responsibilities and participating as principal investigator and/or co-investigator, I was awarded with several small internal grants for evaluating the mutagenicity and/or genotoxic effects of complex mixtures from drinking water treatment plants in the city of Medellin, Colombia. RESEARCH GRANTS AWARDED: Effects of dexamethasone and Leukotriene B4 in apoptosis response in human neutrophiles and its mediation trough reactive oxygen species (ROS). CODI-UA, 1999 (US$ 5,000) Detection of mutagens in drinking water from Villa Hermosa plant. Effects on human mitochondria. City Environmental Health Office – UA, 1999-2000 (US$ 10,000) Detection of mutacarcinogens in Pantanillo River. Effects on genomic and mitochondrial DNA from eukaryotic cells. CODI-UA, 1999-2000 (US$ 10,000) PROFESSIONAL SOCIETIES Human Anatomy & Physiology Society (HAPS), USA (2014-present) Texas Community College Teachers Association (TCCTA), USA (2004-present) National Association of Biology Teachers (NABT), USA (2004-present) Environmental Mutagen Society (EMS), USA (1992-2004) Colombian Society of Genetics, Colombia (1990-2004) LANGUAGES Spanish: native language English: second language