JOURNAL OF BACTERIOLOGYVOLUME 106 * NUMBER 3 * JUNE 1971

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief'(1975)University ofIllinois, Urbana

R. L. ANDERSON, Editor (1975)Michigan State University, East Lansing

S. G. BRADLEY, Editor (1974)Virginia Commonwealth University, Richmond

ROY CURTISS III, Editor (1974)The University of Tennessee, Oak Ridge

ALLEN G. MARR, Editor (1973)University of California, Davis

Martin Alexander (1972)W. Lane Barksdale (1972)M. E. Bayer (1972)Claire M. Berg (1973)Robert W. Bernlohr (1973)Allan Campbell (1972)A. J. Clark (1972)Royston C. Clowes (1972)V. W. Cochrane (1972)Stanley N. Cohen (1973)S. F. Conti (1972)A. L. Demain (1972)John A. DeMoss (1972)Martin Dworkin (1973)Alan D. Elbein (1973)Ellis Englesberg (1972)Herman Friedman (1972)A. T. Ganesan (1972)Howard Goldfine (1972)Joseph S. Gots (1972)Harlyn 0. Halvorson (1972)F. M. Harold (1973)

Leonard Hayflick (1972)George Hegeman (1971)Charles E. Helmstetter (1972)D. S. Hoare (1971)Joseph T. Holden (1972)John G. Holt (1972)Stanley C. Holt (1971)John Ingraham (1973)John H. Law (1971)Hillel S. Levinson (1972)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Alvin Markovitz (1973)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1972)Richard P. Novick (1973)Leo Parks (1973)H. J. Phaff (1972)Allen T. Phillips (1973)

Jack Preiss (1971)H. V. Rickenberg (1973)John Roth (1973)M. R. J. Salton (1972)Moselio Schaechter (1973)David Schlessinger (1971)W. R. Sistrom (1972)Lucille Smith (1972)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1973)Arthur Weissbach (1971)N. E. Welker (1973)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1973)Barbara E. Wright (1971)Frank E. Young (1973)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 4715 Cordell A ve., Bethesda, Md. 20014

EX OFFICIOR. E. HUNGATE, President (1970-1971)

DONALD E. SHAY, Secretary

The Journal of Bacteriology, a publication of the American Societyfor Microbiology, 4715 Cordell Ave., Bethesda, Md. 20014, is devoted tothe advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is $52 (Postal Union Countries, $54; other foreign, $55) peryear. Single copies are $5.00 (foreign, 5.25). Members of the AmericanSociety for Microbiology may receive the Journal as part of their dues.Correspondence relating to reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts, andgeneral editorial matters should be directed to the ASM PublicationsOffice, 4715 Cordell Ave., Bethesda, Md. 20014. Correspondence fromASM members relating to membership dues, member subscriptions,changes of address, incorrect address, incorrect journals, etc., should be

MORRIS F. SHAFFER, Vice-President (1970-1971)T. J. CARSKI, Treasurer

directed to the American Society for Microbiology, 1913 St., N.W.,Washington, D.C. 20006. Nonmembers of the Society should write toThe Williams & Wilkins Co., 428 E. Preston St., Baltimore, Md. 21202,regarding institution of nonmember subscriptions and nonreceipt of

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Second class postage paid at Baltimore, Md. 21202, and at additionalmailing offices.Made in the United States of America.Copyright © 1971, American Society for MicrobiologyAll Rights Reserved.

Author IndexAkagi, J. M., 876Alexander, M., 938Angelo, N., 1016Aronson, A. I., 1016Azam, Farooq, 915

Ballesta, J.-P. G., 938Balows, Albert, 1029Berg, Claire M., 797Berman-Kurtz, Myra, 724Boatman, Edwin S., 1005Bonney, Robert J., 812Booker, Ben F., 1034Boyle, J. M., 896Bradbeer, Clive, 745, 751Brunel, F., 904

Campbell, L. Leon, 717Carbonell, Luis M., 946Carls, R. A., 848Caudill, Claire P., 808Chan, May, 876Cherest, H., 758Chet, Ilan, 863Cirillo, Vincent P., 915Cove, D. J., 1047

Davis, Betty R., 1029Demohn, V., 835de Robichon-Szulmajster, H., 758Di Girolamo, Paula M., 745, 751Djordjevic, Nadezda, 1043Downey, R. J., 1047Duggan, P. F., 908

Emerson, Suzanne U., 949Exterkate, F. A., 824

Fogel, Sam, 863

Glasgow, James E., 882Goldthwait, D. A., 994Gosling, J. P., 908Greer, Sheldon, 983

Gromkova, Rosa H., 720Guarraia, Leonard J., 890

Hanson, R. S., 848Harrison, Arthur P., Jr., 1031Hill, James C., 819Himes, Richard H., 876Holt, S. C., 1016Hu, A. S. L., 1029Hudnik-Plevnik, Tamara A., 1043

Im, S. W. K., 784Iyer, V. N., 1040

Johnson, Marvin J., 830

Kadner, Robert J., 751Kadota, Hajime, 925Kanetsuna, Fuminori, 946Kenny, George E., 1005Killick, K. A., 931Kirby, E. P., 994Kuo, Shou-Chang, 915

Lai, C. J., 835Lange, Charles F., 1034Layne, Porter, 1029Lin, E. C. C., 724Liu, Chao-min, 830

Mandel, M., 802Merdinger, Emanuel, 1034Miovic, Margaret, 856Mitchell, Ralph, 863

Nakaya, Rintaro, 773

O'Bryan, Corliss, 1031Otten, B. J., 824

Pace, B., 717Palchoudlhury, S. R., 1040Paretsky, D., 920Peck, Harry D., Jr., 890Pittard, J., 784

Pizer, Lewis I., 856, 966, 972

Raanan-Ashkenazi, Orina, 791Reissig, Jose L., 882Richey, David P., 724Ronen, A., 791Rothman, Alvin H., 1026Rownd, R., 773Ruff, W., 994

Scardovi, V., 1036Schenley, R. L., 896Sgorbati, B., 1036Shannon, James L., 1026Sicard, A. M., 904Sicard, N., 904Siddhikol, C., 835Simon, Melvin I., 949Sistrom, W. R., 732Slapikoff, Saul, 739Spitzer, Janet L., 739Streips, Uldis N., 868, 955, 960Stueckemann, J., 920Surdin-Kerjan, Y., 758Swenson, P. A., 896

Tanooka, Hiroshi, 925Terano, Hiroshi, 925Tevethia, Mary J., 802, 803Tosa, Tetsuya, 966, 972

Vaccaro, Dennis, 739Vapnek, Daniel, 983Veerkamp, J. H., 824

Wassenberg, H. W., 824Weinfeld, Herbert, 812Weisblum, B., 835Welker, N. E., 955, 960Wittenberg, T., 732

Young, Frank E., 868

Zani, G., 1036

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AUTHOR INDEX

VOLUME 106

Achtman, Mark, 529Akagi, J. M., 876Alexander, M., 938Alexander, Renee R., 213Angelo, N., 1016Anker, P., 634Arnold, J. D., 276Aronson, A. I., 1016Atkins, J. F., 421Atkinson, Daniel E., 31, 37Azam, Farooq, 915

Ballesta, J.-P. G., 938Balows, Albert, 1029Barbour, Stephen D., 204Barksdale, Lane, 707Barnes, W. G., 276Baron, L. S., 243Barton, Robert W., 508Bean, Barry, 412Berg, Claire M., 297, 797Berger, A. E., 276Berk, Richard S., 250Berman-Kurtz, Myra, 724Bernheimer, A. W., 138Bernlohr, R. W., 375Bhattacharya, A. K., 107Blackburn, T. H., 394Blasi, Francesco, 508Boatman, Edwin S., 1005Bonney, Robert J., 812Booker, Ben F., 1034Borenstein, Shoshana, 58Boyd, John W., 578Boyer, Ernest W., 561Boyle, J. M., 896Bradbeer, Clive, 745, 751Brock, Thomas D., 25Brown, Albert T., 456Brown, K. D., 70Brunel, F., 904Burman, Lars G., 1

Calvo, J. M., 213, 286Campbell, L. Leon, 603, 717Capaldo-Kimball, Florence, 204Capes, N., 286Carbonell, Luis M., 946Carls, R. A., 848Carrick, Lee, Jr., 250Caudill, Claire P., 808Chakravorty, M., 107Chan, May, 876Cherest, H., 758Chet, Ilan, 863Chiancone, E., 138Chibata, Ichiro, 493Cirillo, Vincent P., 915

Clark, Alvin J., 529Cochrane, Vincent W., 301Cohen, Murray, 347Cole, Roger M., 257, 712Cole, Ronald S., 143Conti, S. F., 269Cooper, Stephen, 709Costerton, J. W., 659Cove, D. J., 1047Cox, C. D., Jr., 356Coykendall, Alan L., 192Crawford, Irving P., 331

Davis, Betty R., 1029Demohn, V., 835DeMoss, R. D., 97, 283de Robichon-Szulmajster, H., 758Deutch, Charles E., 197DeVoe, I. W., 659DeVries, JoAnne K., 150Di Girolamo, Paula M., 745, 751Djordjevic, Nadezda, 1043Doi, Roy H., 305Downey, R. J., 1047Duggan, P. F., 908Dul, Michael J., 672Dworkin, Nomi B., 543

Easterling, S. B., 243Emerson, Suzanne U., 949Ephrati-Elizur, Erela, 58Exterkate, F. A., 824

Falkow, Stanley, 294Ferretti, Joseph J., 691Fisher, Clark A., 707Fisher, William D., 626Flesher, A., 276Fogel, Sam, 863Forlani, L., 138Fountain, R. H., 65Fralick, Joe A., 626Frank, Hilmer A., 292Freundlich, M., 213Friedberg, Errol C., 500Friedman, Mischa E., 289

Gahan, P., 634Gasser, Charlotte, 113Gasser, F., 113Gherna, R. L., 687Gibson, F., 51Glasgow, James E., 882Goebel, Werner, 311Goering, R. V., 157Goldberger, Robert F., 508Goldthwait, D. A., 994Goodman, M., 286

Gordee, Robert S., 168Gosling, J. P., 908Gott, Cora L., 438, 444Grandgenett, Duane P., 551Greeb, J., 421Greer, Sheldon, 615, 983Greppin, H., 634Gromkova, Rosa H., 720Guarraia, Leonard J., 890Gunsalus, I. C., 468

Haapala, Daniel K., 294Hageage, G. J., Jr., 687Hanson, R. S., 848Harrison, Arthur P., Jr., 1031Hartline, Richard A., 468Hartman, Paul A., 561Haschke, Richard H., 603Hashimoto, T., 269Henry, Susan A., 174Herman, Robert K., 543Hershberger, C., 238Higgins, I. J., 702Highton, Peter J., 646Hill, James C., 819Himes, Richard H., 876Hirota, Yukinori, 523Hobbs, D. G., 646Hoch, J. A., 97Hoffmeyer, J., 14Holt, S. C., 1016Horgen, Paul A., 281Horng, Jong-Sin, 168Howard, Mary B., 289Hu, A. S. L., 1029Hudnik-Plevnik, Tamara A., 1043Hughes, R. C., 694

Im, S. W. K., 784Inouye, Masayori, 539Iyer, V. N., 1040

Jahnke, Linda, 596Jensen, Thomas E., 683Johnson, E. M., 243Johnson, Marvin J., 830Jones, H. E., 339Jones, Lily A., 386Joyce, Gay H., 403

Kadner, Robert J., 751Kadota, Jajime, 925Kanetsuna, Fuminori, 946Katagiri, M., 369Keith, Alec D., 174Kenny, George E., 1005Killick, K. A., 931King, John J., 500

AUTHOR INDEX

Kirby, E. P., 994Kisumi, Masahiko, 493Klein, Harold P., 596Klug, M. J., 283Komatsubara, Saburo, 493Kovach, John S., 508Kulzyck, Sandra, 126Kuo, S.-C., 183, 915

Lacroute, Francois, 519Lai, C. J., 835Lampen, J. O., 183Lange, Charles F., 1034Langman, L., 51Langvad, Finn, 679Layne, Porter, 1029LeBlanc, Donald J., 82, 90Leonard, C. Gomez, 257Leonard, Thomas J., 162Lerud, R. F., 571Lesk, E. M., 394Leutgeb, Werner, 588Liao, Cheng-Liang, 31, 37Lin, E. C. C., 724Lindstrom, E. S., 700Liu, Chao-min, 830London, Jack, 126Loper, J. C., 421Lovett, Paul S., 697Luke, R. K. J., 51

Maas, W. K., 150McDonald, William C., 672MacLeod, Robert A., 659Mandel, M., 608, 802Martin, Robert G., 227Maurer, Richard, 331Meinke, William J., 386Merdinger, Emanuel, 1034Meyer, Eleanor Y., 126Mickel, S., 238Mindich, Leonard, 514Miovic, Margaret, 856Mitchell, Ralph, 640, 863Mortlock, Robert P., 82, 90

Nakaya, Rintaro, 773Neuhard, J., 14Nordstrom, Kurt, 1

O'Bryan, Corliss, 1031Ohta, Y., 702Otten, B. J., 824

Pace, B., 717

Padan, Etana, 45Palchoudhury, S. R., 1040Panos, Charles, 347Paretsky, D., 920Pattee, P. A., 157Pauling, Crellin, 197Payne, W. J., 356Peck, Harry D., Jr., 890Phibbs, P. V., Jr., 375Phillips, Arthur W., 578Pittard, J., 784Pizer, Lewis I., 856, 966, 972

Qadri, S. M. Hussain, 438, 444

Raanan-Ashkenazi, Orina, 791Raboy, Bilha, 45Radke, Kathryn L., 432Rado, Thomas A., 301Randolph, M. L., 221Ranhand, Jon M., 257, 712Ray, Paul H., 25Reissig, Jose L., 882Ribbons, D. W., 702Richey, David P., 724Riley, P. S., 356Ronen, A., 791Rossi, John J., 297Rossier, A., 634Roth, C. W., 97Rothman, Alvin H., 1026Rothman-Denes, Lucia, 227Rownd, R., 238, 773Ruff, W., 994Russell, A. D., 65

Salgo, M., 286Samsonoff, William A., 269Scardovi, V., 1036Schenley, R. L., 896Schneider, R. P., 479, 487Schrempf, Hildgund, 311Schwarz, Uli, 588Scott, Randolph H., 438Sgorbati, B., 1036Seidler, Ramon J., 608Setlow, Jane K., 221Shannon, James L., 1026Shilo, Moshe, 45Shull, Fred W., Jr., 626Sicard, A. M., 904Sicard, M., 904Sicko, Linda M., 683Siddhikol, C., 835

Siegel, Eli C., 432Simon, Melvin I., 949Sinensky, Michael, 449Sirotnak, F. M., 318Sistrom, W. R., 732Slapikoff, Saul, 739Spence, Kemet D., 325Spitzer, Janet L., 739Stahly, Donald P., 551Stokes, Elaine, 694Stratton, Lewis P., 626Streips, Uldis N., 868, 955, 960Stroun, M., 634Stueckemann, J., 920Surdin-Kerjan, Y., 758Swenson, P. A., 896Szaniszlo, Paul J., 640

Tanooka, Hiroshi, 925Terano, Hiroshi, 925Tevethia, Mary J., 802, 808Tomasz, Alexander, 412Tonaki, Keith I., 292Tosa, Tetsuya, 966, 972

Vaccaro, Dennis, 739Van Etten, James L., 704Vapnek, Daniel, 615, 983Veerkamp, J. H., 824

Wassenberg, H. W., 824Weinfeld, Herbert, 812Weisblum, B., 835Welker, N. E., 955, 960Wheelis, M. L., 369White, David C., 25, 403Whiteley, H. R., 571Wiley, W. R., 479, 487Willecke, Klaus, 514Willetts, Neil, 529Williams, Robert P., 438, 444Winkler, Herbert H., 362Wittenberg, T., 732Wittenberger, Charles L., 456Wong, David T., 168.Wuesthoff, G., 709

Yamakawa, Toshio, 305Yoch, Duane C., 700Yoshikawa, Masanosuke, 523Young, Frank E., 697, 868Young, I. G., 51

Zani, G., 1036

*-

J. BACTERIOL.

SUBJECT INDEX

VOLUME 106

Acetate oxidation, adaptation to-enzyme activities during, 908Acetyl-coenzyme A synthetase-in S. cerevisiae, 596N-acetylglucosamine-in S. aureus, 65Acid fastness-elimination of, 707Acinetobacter calcoaceticus-enol-lactone hydrolases from, 369Actinophage MSP2-infection of S. venezuelae, 386Adenosine triphosphatase-DNP stimulation of, 890-in extracts of D. gigas, 890S-adenosylmethionine-in S. cerevisiae, 325Aerobic yeast cells-acetyl-CoA synthetase in, 596Agrobacterium tumefaciens-crown gall induction, 634Agrobacteriwn twnefaciens ribonucleic acid-synthesis in tomato cells, 634Alkane oxidation-by particulate preparation, 830-in Candida, 830Alternative vital role-for NADPH-nitrate reductase gene, 1047-in A. nidulans, 1047Amino acids-in E. coli, 70a-Aminobutyric acid-resistant mutants-of S. marcescens, 493Ampicillin resistance-in E. coli K-12, 1a-Amylase-of S. equinus, 561Antibiotic-induced thermal resistance-in B. subtilis, 672Antimetabolite action-biochemical bases, 972-of L-serine hydroxamate, 972Appendage development-in C. bifermentans, 269D-Arabinose-in E. coli, 90-metabolism of, 82, 90D-Arabinose metabolism-new pathway in E. coli, 90L-Arabinose-production of epimeraseless yeast by, 915L-Arabinose isomerase-in S. typhimuriwn, 107-induction of, 107-repression of, 107Aromatic amino acid pool-in E. coli, 70-maintenance and exchange of, 70

L-Asparaginase-from S. marcescens, 578Aspergillus nidulans-NADPH-nitrate reductase gene, 1047Autolysate-group H Streptococcus, 712Autolytic activity-associated with group H streptococci. 257Auxotroph accumulation-in DNA polymeraseless E. coli, 797Auxotroph recovery in Escherichia coli, 297Azotobacter vinelandii-phosphoenolpyruvate branch-point in, 31, 37-phosphoenolpyruvate carboxylase, 31-pyruvate kinase, 37

Bacillus alvei-indole excretion in, 97Bacillus brevis-sporulation, 739Bacillus cereus T-extracellular protease production in, 1016Bacillus cereus T mutant-pseudogermination in, 292Bacillus licheniformis-cell wall growth, 694-electron microscopy study of, 646-glutamic dehydrogenase of, 375-penicillin and cell wall synthesis, 646Bacillus pwnilus-linkage groups, 697Bacillus stearothermophilus-competence-inducing factor, 868, 955-factors in transfection, 960Bacillus subtilis-chromosome replication in, 58-citrate transport in, 514-effects ofpH, 802,808-growth and sporulation, 551-minor threonine dehydratase, 983-morphology of vegetative cells, 672-sporulation messenger RNA, 305-thermal resistance of vegetative cells, 672-threonine dehydratase-deficient mutants, 615-transcription of light DNA strands, 305-transformation of, 802, 808-vegetative cells, 672-with single-stranded DNA, 802, 808Bacillus subtilis flagellins-variation in primary structure, 949Bacillus subtilis mutants-tricarboxylic acid cycle, 848Bacillus subtilis spores-germinating, 925,-repair of DNA, 925Bacterial deoxyribonucleic acid-transforming ability, 904

iii

SUBJECT INDEX

Bacterial predators-detection of microbial prey by, 863Bacteriophage M13-elimination of F' factors by, 1040Bacteriophage of Proteus mirabilis-R-factor transduction by, 773Bacteriophage PBSl transduction-in B. pumilus, 697Bacteriophage resistance-in E. coli K-12, 432Bacteriophage T4-dark repair of UV-irradiated DNA by, 500Bacterium, sulfate-reducing, 339Bacteroides amylophilus-protease of, 394Bakers' yeast-production by L-arabinose, 915Basidiomycete-new structures, 679Benzo(a)pyrene-effect on S. aureus metabolism, 403-in S. aureus, 403Bifidobacterium-fructose-6-phosphate phosphoketolase in, 1036Bifidobacterium strains-phospholipid composition, 824Biochemical bases-for antimetabolite action, 972-Of L-serine hydroxamate, 972Blastocladiella emersonli-RNA synthesis in, 281-zoospores of, 281Blastomyces dermatitidis-cell wall composition, 946-mycelial forms, 946-yeastlike forms, 946Blue-green algae-endogenous dark respiration of, 45-P. boryanwn, 45-poly-hydroxybutyric acid in, 683Budding cells of Candida albicans-transverse septum formation in, 1026

Camphor degradation-by P. putida, 468-induction for, 468Cancerous plant tumor-induced by A. twnefaciens, 634Candida-alkane oxidation, 830-particulate preparation from, 830Candida albicans-morphological stages of, 276-scanning electron microscopic studies of, 276-transverse septum formation, 1026Capsule formation-inhibition in E. coli K-12, 432Catabolic pathway-specificity of, 702Cell division-in E. coli, 994Cell wall-freeze-etching of single cleavage plane in, 659Cell wall composition-of Blastomyces dermatitidis, 946

Cell wall growth-in B. licheniformis, 694-mucopeptide synthesis, 694Cell wall polysaccharide biosynthesis-in S. pyogenes, 347Cell wall synthesis-in B. licheniformis, 646Cell walls of yeast-culture in presence of sulfur amino acids, 931-modification of structure, 931Cephalosporins-in E. coli, 65-mechanism of action of, 65Chemical detection-of microbial prey, 863Chloramphenicol-in study of DNA synthesis, 709Chlorogloea fritschii-poly-3-hydroxybutyric acid, 683Chorismate mutase P-prephenate dehydratase-mutants derepressed for, 784Chromatium-rhodanese activity in, 700Chromatium okenii-surface structure, 687Chromatium weissei-surface structure, 687Chromosomal integration-of F' factors, 150Chromosome replication-in B. subtilis, 58Citrate transport-in B. subtilis, 514Clostridium bifermentans-appendage development in, 269Colicin la production-at high temperature, 720Colicin tolerance-in E. coli K-12, 1Competence for transformation-by native DNA, 808-by single-stranded DNA, 808Competence-inducing factor-mode of action, 868-of B. stearothermophilus, 955, 868Coniophora cerebella-basidiomycete, 679-new structures, 679Conjugally acquired deoxyribonucleic acid-in E. coli minicells, 626Conjugational transfer-in E. coli, 529Coordinateness of induction-enzymes of E. coli, 812Cortisol-effects during Q fever, 920Crown gall-induced by A. tumefaciens, 634Cyanocobalamin-transport in E. coli mutants, 751

Dark endogenous respiration-of P. boryanum, 45Dark repair-by bacteriophage T4, 500

iV J. BACTERIOL.

SUBJECT INDEX

Dark repair-of UV-irradiated DNA, 500Deoxyribonucleic acid-bacterial, 904-dark repair of, 500-distribution of guanine plus thymine between

strands of, 238-in Enterobacteriaceae, 238-inactivation of, by X rays, 221-polymeraseless E. coli, 797Deoxyribonucleic acid, circular-from beta-hemolytic E. coli strains, 311-isolation and characterization of, 311Deoxyribonucleic acid dark repair mechanisms-in S. aureus, 157Deoxyribonucleic acid, F' factor-in E. coli, 143Deoxyribonucleic acid renaturation-base composition, 608-chromosome replication, 608-quantitative aspects, 608Deoxyribonucleic acid sedimentation-in E. coli, 1043-in S. typhimuriwn, 1043Deoxyribonucleic acid strands, light-in B. subtilis, 305-preferential transcription of, 305Deoxyribonucleic acid synthesis-use of chloramphenicol in study of, 709Deoxyribonucleic acid, transforming-in H. influenzae, 221Desulfotomaculum genus-in classification of dissimilatory sulfate-reducing

bacteria, 339Desulfovibrio gigas-DNP-stimulated ATP activity, 890-extracts of, T-26, 890Desulfovibrio genus-in classification of dissimilatory sulfate-reducing

bacteria, 339Desulfovibrio species-rRNA homology, 717-see D. vulgaris, 717Desulfovibrio vulgaris-rRNA homology, 717-see Desulfovibrio species, 717-thiosulfate reductase of, 603Detection, chemical-microbial prey, 863Diaminopimelate decarboxylase-control by L-lysine, 551Dihydrofolate reductase-evidence for increased synthesis, 318-in D. pnewnoniae, 3182, 3-Dihydroxybenzoate-E. coli mutants unable to synthesize, 51Dinitrophenol-ATP activity stimulated by, 890-in D. gigas extracts, 890-in extracts of D. gigas, 890Diplococcus pnewnoniae-dihydrofolate reductase levels in, 318-marker efficiencies, 904-thymidine starvation, 904

Drug-resistance transfer factor-in Proteus, 294

Electron microscopic studies-of C. albicans, 276Embden-Meyerhof pathway-in S. faecalis, 456Endonuclease-dimer-specific, phage-induced, 500-purification and characterization of, 500Enol-lactone hydrolases-from A. calcoaceticus, 369Enterobacteriaceae-guanine plus thymine distribution in DNA strands,

238Enterochelin-biosynthesis in E. coli, 51Enterotoxin A-production in S. aureus 100, 289Enzyme activities-after acetate oxidation, 908Enzymes-L-asparaginase, 578-a-ketogutarate reductase, 571-of orcinol pathway, 702Epimeraseless yeast-production by L-arabinose, 915Erythromycin-inducible resistance-in S. aureus, 835Escherichia coil-D-arabinose metabolism in, 82, 90-aromatic amino acid pool in, 70-biosynthesis of enterochelin in, 51-cell division, 994-cephalosporins in, 65-effect of serine hydroxamate, 966-effect of trimethoprim, 856-F' factor DNA from, 143-F' factors in, 150-genetic analysis, 529-glycerol kinase operon, 724-growth of, 204-hydrogen sulfide production in, 1029-incubation in medium lacking thymine, 197-kinase activity in, 82-macromolecular synthesis in, 856-macromolecular synthesis in thymineless medium,

197-a-methylglucoside transport in, 362-minicells, 626-murein structure, 588-mutagenesis by ICR-191, 543-mutants unable to synthesize 2, 3-dihydroxyben-

zoate, 51-phospholipid biosynthesis in, 449-photodynamic killing in, 1031-prophage induction, 994-recA gene, 539-recombination in, 204-recovery of auxotrophs after penicillin enrichment,

297-D-ribulokinase activity in, 82-survival in thymineless medium, 197-viability of, 204-vitamin B12 transport in, 745, 751

VOL. 106, 1971 v

SUBJECT INDEX

Escherichia coli B-lambda resistance in, 791-maltose utilization in, 791Escherichia coli B/r-difference from S. typhimurium in DNA, 1043Escherichia coli beta-hemolytic strains-circular DNA from, 311Escherichia coli K-12-bacteriophage resistance in, 432-DNA polymeraseless strains, 797-inhibition of capsule formation in, 432-phenylalanine biosynthesis in, 784-resistance to ampicillin, 1-thymidine metabolism in, 812-tolerance to colicin, 1Escherichia coli K-12 mutants-derepressed, 784Escherichia coli-Salmonella typhosa hybrids-recombination in, 243Escherichia coli transport mutants-vitamin B12, 751Exogenous citrate catabolism-effect of glutamate, 819-in Neisseria species, 819Extracellular a-amylase-of S. equinus, 561Extracellular protease production-in B. cereus T, 1016Extracellular transglucosylase-of S. equinus, 561

F factor-in E. coli, 529F' factors-chromosomal integration of, 150-from UV-irradiated E. coli, 143-in E. coli, 150-phage M13-induced elimination of, 1040Fatty acid composition-effect of temperature on, 25-in T. aquaticus, 25-of mesophilic clostridia, 876

of psychrophilic clostridia, 876-of thermophilic clostridia, 876Fatty acids, saturated-in N. crassa, 174Flagellins of Bacillus subtilis-variation in primary structure, 9495-Fluropyrimidines derivatives-in pneumococcus, 412-inhibitory effects of, 412-metabolism of, 4125-Fluorouracil-reactivation of UV damage, 896Freeze-etching-of gram-negative bacterium, 659Fructose diphosphatase-in bakers' yeast, 908Fructose-6-phosphate phosphoketolase-in Bifidobacteriwn, 1036-starch gell electrophoresis, 1036Fruiting body formation-in S. commune, 162Fungal spores-ribosomal subunits from, 704

Fungi-Blastocladiella emersonii, 281-M. gypseum, 168Fusorium solani-ribosomal competence in, 301-spore germination in, 301

Gene induction-effect on mutagenesis, 543Genetic heterogeneity-in S. mutans, 192Glucose metabolism-in S. faecalis, 456Glucose transport systems-in N. crassa, 479-kinetic characteristics of, 479Glutamate-effect on exogenous citrate catabolism, 819Glutamic dehydrogenase-of B. licheniformis, 375-purification, properties, and regulation of, 375Glycerol deprival-citrate transport induction, 514Glycerol kinase operon-of E. coli, 724Glyoxylate cycle enzymes-in Bakers' yeast, 908Gram-negative bacterium-freeze-etching in cell wall of, 659Gram positivity-of leprosy bacilli, 707Group H Streptococcus-autolysate of, 712-autolytic activity, 257-competence in, 257-transforming DNA in, 712Growth-involvement of recombination genes, 204Guanine plus thymine-asymmetry in, 238-distribution between DNA strands, 238-in DNA of Enterobacteriaceae, 238

Haemophilus influenzae-transforming DNA in, 221Hepatic glycogen-changes during Q fever, 920Hexose-monophosphate pathway-in S. faecalis, 456High-temperature induction-colicin la, 720hisD Mutants-in S. typhimurium, 421Histidine biosynthesis-in S. typhimurium, 508Histidine operon of Salmonella typhimurium-mutations in, 227-phosphoribosyltransferase in, 227Histidinol dehydrogenase mutants-of S. typhimurium, 421Histidyl transfer ribonucleic acid-in S. typhimurium, 508Homology-of rRNA, 717

V1 J. BACTERIOL.

SUBJECT INDEX

Hyphal wall compositions-of fungi of Leptosphaeria genus, 640Hydrogen sulfide production-extrachromosomal nature of, 1029-in E. coli, 1029

ICR-191-reversion by, 543Immunofluorescence-with mucopeptide-specific antiserum, 694Indole excretion-in B. alvei, 97-physiological studies of, 97Indole-positive organisms-in gut and environment of marine animals, 283Induction-requirements for, 835Initiation of chromosome replication-use of chloramphenicol, 709Invertase formation-by Saccharomyces protoplasts, 183-osmotic regulation of, 183Invertase secretion-by Saccharomyces protoplasts, 183-osmotic regulation of, 183Isolation of mutants-Bacillus subtilis, 848-tricarboxylic acid cycle, 848

a-Ketoglutarate reductase-from M. aerogenes, 571Kinase activity-in E. coli, 82Kinetics of glucose-transport systems, 479

Lactic acid bacteria-malic enzyme from, 126Lactic dehydrogenases-immunological relationships among, 113-in Lactobacillus, 113-in Leuconostoc, 113Lactobacillus-lactic dehydrogenases in, 113Lactobacillus strains-phospholipid composition, 824Lambda resistance-in E. coli B, 791Leprosy bacilli-loss of acid-fastness, 707-gram positivity, 707Leptosphaeria genus-marine fungi of, 640-terrestrial fungi of, 640Leuconostoc-lactic dehydrogenases in, 113Leucyl-transfer ribonucleic acid synthetase-in S. typhimuriwn, 213Linkage groups-in B. punilus, 697-PBS1 transduction, 697Low-level UGA suppression-in S. typhimuriwn, 691L-Lysine-control of DAP, 551

Lysis-resistance of Zygorhynchus species to, 938Lytic enzyme-from S. venezuelae, 386

Macromolecular synthesis-effect of trimethoprim, 856-in E. coli, 856, 197Malic enzyme-biochemical and immunological study of, 126-from lactic acid bacteria, 126Maltose utilization-in E. coli B, 791-temperature sensitivity, 791Marine animals-indole-positive organisms in gut and environment

of, 283Marine environment-tryptophanase-positive bacteria in, 283Marine fungi-hyphal wall compositions of, 640-of Leptosphaeria genus, 640Marker efficiencies-in D. pnewnoniae, 904Membrane association-conjugally transferred DNA, 626Membranous inclusions-of P. aeruginosa, 250Mesophilic clostridia-fatty acid composition, 876Mesosomes-of P. aeruginosa, 250Messenger ribonucleic acid, sporulation-of B. subtilis, 305Metabolism of purine bases and nucleosides-in S. typhimurium, 14Methionine-mediated repression-in S. cerevisiae, 758ca-Methylglucoside transport-efflux and steady state in, 362-in E. coli, 362Microbial prey-chemical detection, 863Micrococcus aerogenes-a-ketoglutarate reductase from, 571Microsporum gypseum-effect of pyrrolnitrin on, 168-respiratory chain of, 168Minicells-E. coli, 626Minor threonine dehydratase-in B. subtilis, 983Morphogenetic aspects-murein structure, 588Mucopeptide-specific antiserum-immunofluorescence with, 694Mucopolysaccharide-growth of Neurospora, 882Murein structure-in E. coli, 588Mutagenesis in Escherichia coli-by ICR-191, 543Mutant of Rhodopseudomonas spheroides-anaerobic, 732

*iiVOL. 106, 1971

SUBJECT INDEX

Mutants of Bacillus subtilis-threonine dehydratase-deficient, 615-tricarboxylic acid cycle, 848Mutation, non-Mendelian, 519Mycelial forms-Blastomyces dermatitidis, 946Mycoplasma pnewnoniae spherules-morphology and ultrastructure, 1005

NADPH-nitrate reductase gene-in A. nidulans, 1047Native deoxyriboniucleic acid-competence for transformation, 808Neisseria meningitidis-exogenous citrate catabolism, 819Neisseria species-effect of glutamate, 819-exogenous citrate catabolism, 819Neurospora-mucopolysaccharide, 882-regulation of growth, 882Neurospora crassa-glucose transport systems in, 479-saturated fatty acid requirer of, 174-sugar transport in, 487Nitric oxide-reducing fraction from Pseudomonasperfectomarinus, 356

Nitrite-reducing fraction from Pseudomonas perfec-tomarinus, 356

Nitrous oxide-reducing fraction from Pseudomonasperfectomarinus, 356

Non-Mendelian mutation-ureidosuccinic acid uptake, 519Nucleoside metabolism-in S. typhimuriwn, 14Nucleotide levels-in E. coli cell division, 994Nucleotide synthesis-in S. typhimuriwn, 14

Obligate phototrophic mutants-of R. spheroides, 732Orcinol hydroxylase-pathway of, 702Osmotic regulation of yeast protoplasts, 183

Particulate preparation-alkane oxidation, 830-from Candida, 830PBS1-mediated transduction-in B. pwnilis, 697Penicillin enrichment-in E. coli, 297-recovery of auxotrophs after, 297Penicillin synthesis-in B. licheniformis, 646Phenoloxidase activity-in S. commune, 162Phenylalanine biosynthesis-in E. coli K-12, 784Phosphoenolpyruvate branchpoint-in A. vinelandii, 31, 37-regulation at, 31, 37Phosphoenolpyruvate carboxylase-in A. vinelandii, 31

Phosphoenolpyruvate synthase activity-Salmonella locus affecting, 286Phospholipid biosynthesis-in E. coli, 449-temperature control of, 449Phospholipid composition-Bifidobacterium strains, 824-Lactobacillus strains, 824Phospholipid synthesis-absence of, 514-citrate transport, 514Phosphoribosyltransferase-binding to His-tRNA, 508-in histidine operon of S. typhimurium, 227Photodynamic killing-variability in E. coli, 1031Photosynthetic bacteria-rhodanese activity, 700Phototrophic mutants-of R. spheroides, 732Pigmentation-in S. marcescens, 438, 444-influence of growth medium on, 438-influence of temperature of incubation on, 438Piperonyl butoxide-effect on S. aureus metabolism, 403-in S. aureus, 403Plectonema boryanum-blue-green alga, 45-endogenous dark respiration of, 45Pleiotropic effect-recA gene of E. coli, 539Pleiotropic regulatory system-in S. cerevisiae, 758-involving methionyl tRNA, 758-involving product of gene eth2, 758Pneumococcus-5-fluoropyrimidine derivatives in, 412Poly-,3-hydroxybutyric acid-in Chlorogloea fritschii, 683Poly-g-hydroxybutyric acid granules-fine structure, 683Polyhydroxybutyrate particles-of P. aeruginosa, 250Polymerase-deficient auxotrophs-of E. coli K-12, 797Polysaccharide biosynthesis-in S. pyogenes, 347Primary structure-B. subtilis flagellins, 949Promoter-like mutant-of E. coli, 724Prophage induction-in E. coli, 994Protease-of B. amylophilus, 394Protease production-by mutants of B. cereus T, 1016-in B. brevis, 739Protein-changes during Q fever, 920Protein turnover-in B. brevis, 739Proteus

vii J. BACTERIOL.

SUBJECT INDEX

-drug-resistance transfer factor in, 294Proteus mirabilis bacteriophage-R-factor transduction by, 773Pseudomonas aeruginosa-membranous inclusions of, 250-mesosomes of, 250-polyhydroxybutyrate particles of, 250Pseudomonas perfectomarinus-nitric oxide-reducing fractions, 356-nitrite-reducing fractions, 356-nitrous oxide-reducing fractions, 356Pseudomonas putida-camphor degradation by, 468-new regulatory mutation in, 331-tryptophan genes in, 331Psychrophilic clostridia, 876Pullularia pullulans-trehalase from, 1034Purine base metabolism-in S. typhimuriwn, 14Pyridine extraction-of leprosy bacilli, 707Pyridine nucleotides-role in reactivation, 896Pyrrolnitrin-effect on M. gypseum, 168Pyruvate kinase-in A. vinelandii, 37

Q fever-changes in hepatic glycogen during, 920-changes in protein during, 920-changes in RNA synthesis during, 920-effects of cortisol, 920

R factor-in P. mirabilis, 294-transducible by Plkc, 523R factor transduction, 523, 773Radiation damage-repair of DNA, 925Reactivation-5-fluorouracil-mediated, 896-of UV damage, 896Rec- Hfr strains-F' integration in, 150recA gene of Escherichia coli-pleiotropic effect, 539recA- mutation-non-essentiality of, 1040Recombination-in E. coli, 204-in E. coli-S. typhosa hybrids, 243Regulation-thymidine metabolism, 812Regulation of growth-by mucopolysaccharide, 882-in Neurospora, 882Renaturation of deoxyribonucleic acid-quantitative aspects, 608Repair of radiation damage-to DNA in spores, 925Repression, methionine-mediated-in S. cerevisiae, 758Resistance in Staphylococcus aureus

-erythromycin-inducible, 835Resistance to lysis-Zygorhynchus species, 938Rhodanese activity-in photosynthetic bacteria, 700Rhodopseudomonas palustris-rhodanese activity in, 700Rhodopseudomonas spheroides-anaerobic mutant, 732Ribonucleic acid synthesis-changes during Q fever, 920Ribonucleic acid synthesis, in vitro-in B. emersonii, 281Ribosomal competence-in F. solani, 301Ribosomal ribonucleic acid-homology, 717Ribosomal subunits-biologically active, 704-from fungal spores, 704D-Ribulokinase activity-in E. coli, 82-origin of, 82

Saccharomyces cerevisiae-acetyl-Co A synthetase, 596-methionine-mediated repression, 758-mutant preventing S-adenosylmethionine uptake

in, 325-non-Mendelian mutants, 519Saccharomyces protoplasts-osmatic regulation of, 183Sacculus-in E. coli, 588Salmonella locus-affecting phosphoenolpyruvate synthase activity,

286Salmonella typhimurium-altered leucyl-tRNA synthetase in, 213-L-arabinose isomerase in, 107-histidine biosynthesis in, 508-histidinol dehydrogenase mutants of, 421-histidyl transfer RNA in, 508-metabolism of purine bases and nucleosides, 14-mutants of, 213-nucleotide synthesis in, 14-phosphoribosyltransferase in, 227-recombination in hybrids between E. coli, 243-UGA triplet, 691Salmonella typhimurium LT-2-difference from E. coli in DNA, 1043Salmonella typhimurium mutations, 227Schizophyllum commune-fruiting body formation, 162-phenoloxidase activity, 162Sedimentation behavior-of DNA in E. coli, 1043-of DNA in S. typhimurium, 1043L-Serine hydroxamate-antimetabolite action, 972Serine hydroxamate-effect on growth of E. coli, 966Serratia marcescens-a-aminobutyric acid-resistant mutants of, 493-L-asparaginase from, 578

VOL. 106, 1971 iX

SUBJECT INDEX

-induction of pigmentation in, 444-pigmentation in, 438Serratia marcescens mutants-valine accumulation by, 493Shigella sonnei-colicin la production in, 720Single-stranded deoxyribonucleic acid-competence for transformation, 808-transformation of B. subtilis cells, 802, 808Spherules of Mycoplasma pnewnoniae-morphology and ultrastructure, 1005Spore formation-in B. subtilis, 551Spore germination-in F. solani, 301Spores-appendage development in C. bifermentans, 269-lacking dipicolinic acid, 292-of a B. cereus T mutant, 292Spores of Bacillus subtilis-germinating, 925-repair of DNA, 925Sporulation-in B. brevis, 739Sporulation messenger ribonucleic acid-of B. subtilis, 305Staphylococcal alpha toxin-ultracentrifugal analysis of, 138Staphylococcus aureus-N-acetylglucosamine in, 65-DNA dark repair mechanisms, 157-effect of benzo(a)pyrene in, 403-effect of piperonyl butoxide in, 403-erythromycin-inducible resistance in, 835-sensitivity to UV radiation, 157Staphylococcus aureus 100-increased ability to produce enterotoxin A, 289-mutants of, 289Starch gel electrophoresis-in Bifidobacteriwn, 1036-of fructose-6-phosphate phosphoketolase, 1036Streptococci, group H-autolytic activity, 257-competence in, 257Streptococcus, group H-autolysate, 712-transforming DNA in, 712Streptococcus equinus-extracellular ac-amylase of, 561-extracellular transglucosylase of, 561Streptococcus faecalis-Embden-Meyerhof pathway in, 456-glucose metabolism in, 456-hexose-monophosphate pathway in, 456Streptococcus mutans-DNA base contents of, 192-genetic heterogeneity in, 192Streptococcus pyogenes-cell wall polysaccharide biosynthesis in, 347Streptomyces venezuelae-infected by actinophage MSP2, 386-lytic enzyme from, 386Sugar transport-in N. crassa, 487-regulation of. 487

Sulfate-reducing bacterium-with unusual morphology and pigment content, 339Sulfur amino acids-effect on yeast cell wall, 931Surface structure-Chromatium okenii, 687-Chromatium weissei, 687

Temperature sensitivity-of maltose utilization, 791Terrestrial fungi-hyphal wall compositions of, 640-of Leptosphaeria genus, 640Thermal resistance-in vegetative cells of B. subtilis, 672Thermophilic clostridia-fatty acid composition, 876Thermus aquaticus-fatty acid composition, 25Thiosulfate reductase-of D. vulgaris, 603Threonine dehydratase-in B. subtilis, 983-in threonine synthetic region, 983Threonine dehydratase-deficient mutants-of B. subtilis, 615Threonine synthetic region-of B. subtilis, 983Thymidine metabolism-regulation in E. coli K-12, 812Thymidine starvation-in D. pneumoniae, 904-marker efficiencies during, 904Thymine-independent Bacillus subtilis-chromosome replication in, 58Thymine mutant-of E. coli, 192Thymine-requiring Bacillus subtilis-chromosome replication in, 58Tomato cells-synthesis of A. tumefaciens RNA in, 634Transduction-R factors, 773Transduction, PBS1-mediated-in B. pumilus, 697Transduction of R213-by Plkc, 523Transfection-factors affecting, 960-in Bacillus stearothermophilus, 960Transfer-deficient mutants-in E. coli, 529Transformation-effects ofpH, 802-of B. subtilis, 802, 808Transforming deoxyribonucleic acid-in autolysate of group H Streptococcus, 712Transglucosylase-of S. equinus, 561Transport of vitamin B12-in E. coli, 745, 751Transverse septum formation-in budding cells of C. albicans, 1026Trehalase from Pullularia pullulans-isolation and identification, 1034

x J. BACTERIOL.

SUBJECT INDEX

Tricarboxylic acid cycle enzymes-in bakers' yeast, T-29, 908Tricarboxylic acid cycle mutants-of B. subtilis, 848Trimethoprim-effect on macromolecular synthesis, T-21, 856Tryptophan genes-in P. putida, 331Tryptophanase-positive bacteria-in marine environment, 283

UGA triplet-in S. typhimuriwn, 691Ultracentrifugal analysis-of staphylococcal alpha toxin, 138Ultraviolet-irradiated deoxyribonucleic acid-dark repair by bacteriophage T4, 500Ultraviolet-irradiated Escherichia coli-F' factors from, 143Ultraviolet radiation-sensitivity in S. aureus, 157Ultraviolet radiation damage-reactivation, 896Ultraviolet-sensitive mutants-of S. aureus, 157Uncoupling of cell division-from DNA replication, 539Ureidosuccinic acid uptake-in yeast, 519

Valine accumulation-by S. marcescens mutants, 493

Vegetative cells-of B. subtilis, 672Viability-effect of thymine or, in E. coli, 197-involvement of recombination, 204-of E. coli K-12, 204Vibrio genus-isolates from marine environment, 283Vitamin B12-transport in E. coli, 745, 751Vitamin B12 transport mutants-isolation from E. coli KBTOO1, 751

X rays-effect on H. influenzae DNA, 221-inactivation of transforming DNA by, 221

Yeast cell wall-effect of sulfur amino acids, 931-modification of composition, 931-modification of structure, 931Yeast protoplasts-osmotic regulation of, 183Yeastlike forms-Blastomyces dermatitidis, 946Yeastlike fungus-transverse septum formation in, 1026

Zoospores-of B. emersonii, 281Zygorhynchus species-resistance to lysis, 938

XiVOL. 106, 1971

INDEX TO DATE OF ISSUE

VOLUME 106

Month Date of Issue Pages

April 31 March 1971 1-296May 3 May 1971 297-716June 8 June 1971 717-1050

JOURNALOF

BACTERIOLOGY

VOLUME 106

BALTIMORE, MD. 21202

1971

JOURNAL OF BACTERIOLOGYVOLUME 106

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief(1975)University ofIllinois, Urbana

R. L. ANDERSON, Editor (1975)Michigan State University, East Lansing

S. G. BRADLEY, Editor (1974)Virginia Commonwealth University, Richmond

ROY CURTISS Ill, Editor (1974)The University of Tennessee, Oak Ridge

ALLEN G. MARR, Editor (1973)University ofCalifornia, Davis

Martin Alexander (1972)W. Lane Barksdale (1972)M. E. Bayer (1972)Claire M. Berg (1973)Robert W. Bernlohr (1973)Allan Campbell (1972)A. J. Clark (1972)Royston C. Clowes (1972)V. W. Cochrane (1972)Stanley N. Cohen (1973)S. F. Conti (1972)A. L. Demain (1972)John A. DeMoss (1972)Martin Dworkin (1973)Alan D. Elbein (1973)Ellis Englesberg (1972)Herman Friedman (1972)A. T. Ganesan (1972)Howard Goldfine (1972)Joseph S. Gots (1972)Harlyn 0. Halvorson (1972)F. M. Harold (1973)

Leonard Hayflick (1972)George Hegeman (197 1)Charles E. Helmstetter (1972)D. S. Hoare (1971)Joseph T. Holden (1972)John G. Holt (1972)Stanley C. Holt (197 1)John Ingraham (1973)John H. Law (1971)Hillel S. Levinson (1972)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Alvin Markovitz (1973)Julius Marmur (1971)Robert G. Martin (I197 1)M. L. Morse (1972)Richard P. Novick (1973)Leo Parks (1973)H. J. Phaff (1972)Allen T. Phillips (1973)

Jack Preiss (1971)H. V. Rickenberg (1973)John Roth (1973)M. R. J. Salton (1972)Moselio Schaechter (1973)David Schlessinger ( 1971)W. R. Sistrom (1972)Lucille Smith (1972)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1973)Arthur Weissbach (1971)N. E. Welker (1973)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1973)Barbara E. Wright ( 1971)Frank E. Young (1973)Stanley A. Zahler (I197 1)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIOR. E. HUNGATE, President (1970-1971)

DONALD E. SHAY, Secretary

The Journal of Bacteriology, a publication of the American Societyfor Microbiology, 4715 Cordell Ave., Bethesda, Md. 20014, is devoted tothe advancement and dissemination of fundamental knowledge concerningbacteria and other microorganisms. The Journal is published monthly, andthe twelve numbers are divided into four volumes per year. The subscrip-tion price is $52 (Postal Union Countries, s54; other foreign, S55) peryear. Single copies are $5.00 (foreign, 5.25). Members of the AmericanSociety for Microbiology may receive the Journal as part of their dues.Correspondence relating to reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts, andgeneral editorial matters should be directed to the ASM PublicationsOffice, 4715 Cordell Ave., Bethesda, Md. 20014. Correspondence fromASM members relating to membership dues, member subscriptions,changes of address, incorrect address, incorrect journals, etc., should be

MORRIS F. SHAFFER, Vice-President (1970-1971)T. J. CARSKI, Treasurer

directed to the American Society for Microbiology, 1913 1 St., N.W.,Washington, D.C. 20006. Nonmembers of the Society should write toThe Williams & Wilkins Co., 428 E. Preston St., Baltimore, Md. 21202,regarding institution of nonmember subscriptions and nonreceipt ofjournals. Published monthly by the ASM at 428 E. Preston St.,Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at additionalmailing offices.Made in the United States of America.Copyright © 1971, American Society for MicrobiologyAll Rights Reserved.

Volume 106 Contents for April Number 1

Taxonomy and EcologyGenetic Heterogeneity in Streptococcus mutans. ALAN L. COYKENDALL............... 192Tryptophanase-Positive Bacteria in the Marine Environment. M. J. KLUG AND R. D.

DEMOSS................................................................ 283

Morphology and UltrastructureMembranous Inclusions of Pseudomonas aeruginosa. LEE CARRICK, JR., AND RICHARD

S. BERK................................................................. 250

Appendage Development in Clostridium bifermentans. WILLIAM A. SAMSONOFF, T.HASHIMOTO, AND S. F. CONTI .............................................. 269

Scanning Electron Microscopic Studies of Candida albicans. W. G. BARNES, A. FLESHER,A. E. BERGER, AND J. D. ARNOLD.......................................... 276

Genetics and Molecular BiologyVelocity of Chromosome Replication in Thymine-Requiring and Independent Strains of

Bacillus subtilis. ERELA EPHRATI-ELIZUR AND SHOSHANA BORENSTEIN.............. 58Properties of F' Factor Deoxyribonucleic Acid Transferred from Ultraviolet-Irradiated

Donors: Photoreactivation in the Recipient and the Influence of recA, recB, recC,and uvr Genes. RONALD S. COLE ........................................... 143

Chromosomal Integration of F' Factors in Recombination-Deficient Hfr Strains ofEscherichia coli. JOANNE K. DEVRIES AND W. K. MAAS....................... 150

Mutants of Staphylococcus aureus with Increased Sensitivity to Ultraviolet Radiation.R. V. GOERING AND P. A. PATrEE .......................................... 157

Phenoloxidase Activity and Fruiting Body Formation in Schizophyllum commune.THOMAS J. LEONARD...................................................... 162

Survival and Macromolecular Synthesis During Incubation of Escherichia coli inLimiting Thymine. CHARLES E. DEUTCH AND CRELLIN PAULING................ 197

Involvement ofRecombination Genes in Growth and Viability of Escherichia coli K-12.FLORENCE CAPALDO-KIMBALL AND STEPHEN D. BARBOUR...................... 204

Mutants of Salmonella typhimurium with an Altered Leucyl-Transfer RibonucleicAcid Synthetase. RENEE R. ALEXANDER, J. M. CALVO, AND M. FREUNDLICH ........ 213

Mechanism of Inactivation of Transforming Deoxyribonucleic Acid by X Rays. M. L.RANDOLPH AND JANE K. SETLOW........................................... 221

Two Mutations in the First Gene of the Histidine Operon of Salmonella typhimuriumAffecting Control. LUCIA ROTHMAN-DENES AND ROBERT G. MARTIN.............. 227

Asymmetric Distribution of Guanine Plus Thymine Between Complementary Strandsof Deoxyribonucleic Acid of Members of the Enterobacteriaceae. C. HERSHBERGER,S. MICKEL, AND R. ROWND................................................ 238

Inefficiency of Genetic Recombination in Hybrids Between Escherichia coli and Salmo-nella typhosa. E. M. JOHNSON, S. B. EASTERLING, AND L. S. BARON................ 243

Autolytic Activity Associated with Competent Group H Streptococci. JON M.RANHAND, C. GOMEZ LEONARD, AND ROGER M. COLE ......................... 257

In Vitro Ribonucleic Acid Synthesis in the Zoospores of the Aquatic Fungus Blasto-cladiella emersonii. PAUL A. HORGEN ........................................ 281

Salmonella Locus Affecting Phosphoenolpyruvate Synthase Activity Identified by aDeletion Analysis. J. M. CALVO, M. GOODMAN, M. SALGO, AND N. CAPES.......... 286

Induction of Mutants of Staphylococcus aureus 100 with Increased Ability to ProduceEnterotoxin A. MISCHA E. FRIEDMAN AND MARY B. HOWARD.................. 289

Physical Studies of the Drug-Resistance Transfer Factor in Proteus. DANIEL K. HAAPALAAND STANLEY FALKOW.................................................... 294

Physiology and MetabolismColicin Tolerance Induced by Ampicillin or Mutation to Ampicillin Resistance in a

Strain of Escherichia coli K- 12. LARS G. BURMAN AND KURT NORDSTROM .......... 1Metabolism of Exogenous Purine Bases and Nucleosides by Salmonella typhimurium.

J. HOFFMEYER AND J. NEUHARD............................................ 14

xv

CONTENTS

Effect of Temperature on the Fatty Acid Composition of Thermus aquaticus. PAUL H.RAY, DAVID C. WHITE, AND THOMAS D. BROCK.............................. 25

Regulation at the Phosphoenolpyruvate Branchpoint in Azotobacter vinelandii: Phos-phoenolpyruvate Carboxylase. CHENG-LIANG LIAO AND DANIEL E. ATKINSON ...... 31

Regulation at the Phosphoenolpyruvate Branchpoint in Azotobacter vinelandii: PyruvateKinase. CHENG-LIANG LIAO AND DANIEL E. ATKINSON........................ 37

Endogenous Dark Respiration of the Blue-Green Alga, Plectonema boryanum. ETANAPADAN, BILHA RABOY, AND MOSHE SHILO.................................... 45

Biosynthesis of the Iron-Transport Compound Enterochelin: Mutants of Escherichiacoli Unable to Synthesize 2,3-Dihydroxybenzoate. I. G. YOUNG, L. LANGMAN, R.K. J. LUKE, AND F. GIBSON................................................ 51

Aspects of the Mechanism of Action of Some Cephalosporins. A. D. RUSSELL ANDR. H. FOUNTAIN......................................................... 65

Maintenance and Exchange of the Aromatic Amino Acid Pool in Escherichia coli.K. D. BROWN........................................................... 70

Metabolism of D-Arabinose: Origin of a D-Ribulokinase Activity in Escherichia coli.DONALD J. LEBLANC AND ROBERT P. MORTLOCK..................... 82

Metabolism of D-Arabinose: a New Pathway in Escherichia coli. DONALD J. LEBLANcAND ROBERT P. MORTLOCK............................ ................. 90

Physiological Studies of Biosynthetic Indole Excretion in Bacillus alvei. C. W. ROTH,J. A. HOCH, AND R. D. DEMOSS........................................... 97

Induction and Repression of L-Arabinose Isomerase in Salmonella typhimurium. A. K.BHATTACHARYA AND M. CHAKRAVORTY......................ie.............. 107

Respiratory Chain of a Pathogenic Fungus, Microsporum gypseum: Effect of the Anti-fungal Agent Pyrrolnitrin. DAVID T. WONG, JONG-SIN HORNG, AND ROBERT S.GORDEE.. ..................................................... ......... 168

Saturated Fatty Acid Requirer of Neurospora crassa. SUSAN A. HENRY AND ALEC D.KEITH. . . ............................................ ...... ..... . . . 174

Osmotic Regulation of Invertase Formation and Secretion by Protoplasts of Sac-charomyces. S.-C. KUO AND J. 0. LAMPEN................................... 183

Pseudogermination in Dipicolinic Acid-Less Spores of a Bacillus cereus T Mutant.HILMER A. FRANK AND KEITH I. TONAKI.................................... 292

EnzymologyImmunological Relationships Among Lactic Dehydrogenases in the Genera Lacto-

bacillus and Leuconostoc. F. GASSER AND CHARLOTTE GASSER................ 113Comparative Biochemical and Immunological Study of Malic Enzyme from Two

Species of Lactic Acid Bacteria: Evolutionary Implications. JACK LONDON, ELEANORY. MEYER, AND SANDRA KULCZYK......................................... 126

Ultracentrifugal Analysis of Staphylococcal Alpha Toxin. L. FORLANI, A. W. BERN-HEIMER, AND E. CHIANCONE................................................ 138

Volume 106 Contents for May Number 2

Taxonomy and EcologySulfate-Reducing Bacterium with Unusual Morphology and Pigment Content. H. E.

JONES .. ................................................................. 339

Morphology and UltrastructureMorphogenetic Aspects of Murein Structure and Biosynthesis. ULI SCHWARZ AND

WERNER LEUTGEB.......................... 588Hyphal Wall Compositions of Marine and TerrestrialFungi of theGenusLeptosphaeria.

PAUL J. SZANISZLO AND RALPH MITCHELL................................. 640Penicillin and Cell Wall Synthesis: Study of Bacillus licheniformis by Electron Micros-

COPY. PETER J. HIGHTON AND D. G. HOBBS................................. 646

XVi

Demonstration by Freeze-Etching of a Single Cleavage Plane in the Cell Wall of aGram-Negative Bacterium. I. W. DEVOE, J. W. COSTERTON, AND ROBERT A.MAcLEOD............................................................... 659

Morphological Changes and Antibiotic-Induced Thermal Resistance in Vegetative Cellsof Bacillus subtilis. MICHAEL J. DUL AND WILLIAM C. MACDONALD............... 672

New Structures in the Basidiomycete, Coniophora cerebella. FINN LANGVAD ..... 679Fine Structure of Poly-$-Hydroxybutyric Acid Granules in a Blue-Green Alga, Chloro-

gloeafritschii. THOMAS E. JENSEN AND LINDA M. SICKO......................... 683Surface Structure of Chromatium okenii and Chromatium weissei. G. J. HAGEAGE, JR.,

AND R . L. GHERNA ...................................................... 687Elimination of the Acid Fastness but Not the Gram Positivity of Leprosy Bacilli After

Extraction with Pyridine. CLARK A. FISHER AND LANE BARKSDALE. 707

Genetics and Molecular BiologyDifferential Recovery of Auxotrophs After Penicillin Enrichment in Escherichia coli.

JOHN J. RosSI AND CLAIRE M. BERG...................................... 297Ribosomal Competence and Spore Germination in Fusarium solani. THOMAS A. RADO

AND VINCENT W. COCHRANE ....................................... 301Preferential Transcription of Bacillus subtilis Light Deoxyribonucleic Acid Strands

During Sporulation. ToSHIO YAMAKAWA AND RoY H. DoI................... 305Isolation and Characterization of Supercoiled Circular Deoxyribonucleic Acid from

Beta-Hemolytic Strains of Escherichia coli. WERNER GOEBEL AND HILDGUNDSCHREMPF ............................................................... 311

High Dihydrofolate Reductase Levels in Diplococcus pneumoniae After Mutation in theStructural Gene: Biochemical and Immunological Evidence for Increased Synthesis.F. M. SIROTNAK............................................ ...... 318

Mutation of Saccharomyces cerevisiae Preventing Uptake of S-Adenosylmethionine.KEMET D. SPENCE..... ................................325

New Regulatory Mutation Affecting Some of the Tryptophan Genes in Pseudomonasputida. RICHARD MAURER AND IRVING P. CRAWFORD........................ 331

Histidinol Dehydrogenase (hisD) Mutants of Salmonella typhimurium. J. GREEB, J. F.ATKINS, AND J. C. LOPER ................................................ 421

Mutation Preventing Capsular Polysaccharide Synthesis in Escherichia coli K-12 and ItsEffect on Bacteriophage Resistance. KATHRYN L. RADKE AND ELI C. SIEGEL ..... 432

Induction of Citrate Transport in Bacillus subtilis During the Absence of PhospholipidSynthesis. KLAUS WILLECKE AND LEONARD MINDICH......... 514

Non-Mendelian Mutation Allowing Ureidosuccinic Acid Uptake in Yeast. FRANCOISLACROUTE . ...................... ...................................... 519

Impaired Transduction of R213 and Its Recovery by a Homologous Resident R Factor.MASANOSUKE YOSHIKAWA AND YUKINORI HIROTA....................a....... 523

Beginning a Genetic Analysis of Conjugational Transfer Determined by the F Factor inEscherichia coli by Isolation and Characterization of Transfer-Deficient Mutants.MARK ACHTMAN, NEIL WILLETTS, AND ALVIN J. CLARK..................... 529

Pleiotropic Effect of the recA Gene of Escherichia coli: Uncoupling of Cell Divisionfrom Deoxyribonucleic Acid Replication. MASAYORI INOUYE.................... 539

Effect of Gene Induction on the Rate of Mutagenesis by ICR-191 in Escherichia coli.ROBERT K. HERMAN AND NOMI B. DWORKIN............................... 543

Quantitative Aspects of Deoxyribonucleic Acid Renaturation: Base Composition, Stateof Chromosome Replication, and Polynucleotide Homologies. RAMON J. SEIDLERAND M. MANDEL...

. .............. I............... ............ 608Suppression by Derepression in Threonine Dehydratase-Deficient Mutants of Bacillus

subtilis. DANIEL VAPNEK AND SHELDONGREER.615Membrane Association of Conjugally Transferred Deoxyribonucleic Acid in Escherichia

coli Minicells. FRED W. SHULL, JR., JOE A. FRALICK, LEWIS P. STRATTON, ANDWILLIAM D. FISHER............................ ....................... 626

Agrobacterium tumefaciens Ribonucleic Acid Synthesis in Tomato Cells and CrownGall Induction. M. STROUN, P. ANKER, P. GAHAN, A. ROSSIER, AND H. GREPPIN. ... 634

Low-Level Reading of the UGA Triplet in Salmonella typhimurium. JOSEPH J. FER-RETTI................ 691

CONTENTS x*ii

CONTENTS

Linkage Groups in Bacillus pumilus Determined by Bacteriophage PBSl-MediatedTransduction. PAUL S. LOVETT AND FRANK E. YOUNG...................... 697

Preparation of Biologically Active Ribosomal Subunits from Fungal Spores. JAMES L.VAN ETTEN................................. ..............................704

Comment on the Use of Chloramphenicol to Study Initiation of Deoxyribonucleic AcidSynthesis. STEPHEN COOPER AND G. WUESTHOFF... 709

Demonstration of Transforming Deoxyribonucleic Acid in an Autolysate of a GroupH Streptococcus. JON M. RANHAND AND ROGER M. COLE....................... 712

Physiology and Metabolism

Cell Wall Polysaccharide Biosynthesis by Membrane Fragments from Streptococcuspyogenes and Stabilized L-Form. MURRAY COHEN AND CHARLES PANOS............. 347

Separate Nitrite, Nitric Oxide, and Nitrous Oxide Reducing Fractions from Pseudo-monas perfectomarinus. W. J. PAYNE, P. S. RILEY, AND C. D. Cox, JR.............. 356

Efflux and the Steady State in a-Methylglucoside Transport in Escherichia coli. HERBERTH. WINKLER .................................. 362

Effect of Benzo(a)pyrene and Piperonyl Butoxide on Formation of Respiratory System,Phospholipids, and Carotenoids of Staphylococcus aureus. GAY H. JOYCE ANDDAVID C. WHITE....................................................... 403

Inhibitory Effects and Metabolism of 5-Fluoropyrimidine Derivatives in Pneumococcus.BARRY BEAN AND ALEXANDER TOMASZ..................................... 412

Influence of Temperature of Incubation and Type of Growth Medium on Pigmentationin Serratia marcescens. ROBERT P. WILLIAMS, CORA L. GOTT, S. M. HUSSAIN QADRI,AND RANDOLPH H. SCOTT ................................................ 438

Induction of Pigmentation in Nonproliferating Cells of Serratia marcescens by Additionof Single Amino Acids. ROBERT P. WILLIAMS, CORA L. GOTT, AND S. M. HUSSAINQUADRI . ....................................... 444

Temperature Control of Phospholipid Biosynthesis in Escherichia coli. MICHAEL SINEN-SKY................................................................... 449

Mechanism for Regulating the Distribution of Glucose Carbon Between the Embden-Meyerhoff and Hexose-Monophosphate Pathways in Streptococcus faecalis. ALBERTT. BROWN AND CHARLES L. WITTENBERGER .................... 456

Induction Specificity and Catabolite Repression of the Early Enzymes in CamphorDegradation by Pseudomonas putida. RICHARD A. HARTLINE AND I. C. GUNSALUS. . . 468

Kinetic Characteristics of the Two Glucose Transport Systems in Neurospora crassa. R.P. SCHNEIDER AND W. R. WILEY ........... 479

Regulation of Sugar Transport in Neurospora crassa. R. P. SCHNEIDER AND W. R.WILEY.................................................................. 487

Valine Accumulation by a-Aminobutyric Acid-Resistant Mutants of Serratia marcescens.MASAHIKO KISUMI, SABURO KOMATSUBARA, AND ICHIRO CHIBATA............. 493

Cell Wall Growth in Bacillus licheniformis Followed by Immunofluorescence with Muco-peptide-Specific Antiserum. R. C. HUGHES AND ELAINE STOKES............... 694

Survey of the Photosynthetic Bacteria for Rhodanese (Thiosulfate:Cyanide SulfurTransferase) Activity. DUANE C. YOCH AND E. S. LINDSTROM.................... 700

Specificity of a Catabolic Pathway-a Lesson Learned from Indirect Assays. D. W.RIBBONS, Y. OHTA, AND I. J. HIGGINS..................................... 702

Enzymology

Comparison of the Two Isofunctional Enol-Lactone Hydrolases from Acinetobactercalcoaceticus. M. KATAGIRI AND M. L. WHEELIS............................ 369

Purification, Properties, and Regulation of Glutamic Dehydrogenase of Bacillus licheni-formis. P. V. PHIBBS, JR., AND R. W. BERNLOHR................................ 375

Lytic Enzyme from Lysates of Streptomyces venezuelae Infected with ActinophageMSP2. WILLIAM J. MEINKE AND LILY A. JONES............................ 386

Purification of Bacteroides amylophilus Protease. E. M. LESK AND T. H. BLACKBURN. .. 394Dark Repair of Ultraviolet-Irradiated Deoxyribonucleic Acid by Bacteriophage T4:

Purification and Characterization of a Dimer-Specific Phage-Induced Endonu-clease. ERROL C. FRIEDBERG AND JOHN J. KING............................ 500

xviii

CONTEN1i

Interaction Between the First Enzyme for Histidine Biosynthesis and Histidyl TransferRibonucleic Acid. FRANCESCO BLASI, ROBERT W. BARTON, JOHN S. KOVACH, ANDROBERT F. GOLDBERGER.................................................. 508

Control of Diaminopimelate Decarboxylase by L-Lysine During Growth and Sporula-tion of Bacillus cereus. DUANE P. GRANDGENETT AND DONALD P. STAHLY.......... 551

Extracellular Transglycosylase and c-Amylase of Streptococcus equinus. ERNEST W.BOYER AND PAUL A. HARTMANN .......................................... 561

Purification and Properties of a-Ketoglutarate Reductase from Micrococcus aerogenes.R. F. LERUD AND H. R. WHITELEY ....................................... 571

Purification and Properties of L-Asparaginase from Serratia marcescens. JOHN W.BOYD AND ARTHUR W. PHILLIPS .......................................... 578

Variations in the Localization of Acetyl-Coenzyme A Synthetase in Aerobic Yeast Cells.HAROLD P. KLEIN AND LINDA JAHNKE ..................................... 596

Thiosulfate Reductase of Desulfovibrio vulgaris. RICHARD H. HASCHKE AND L. LEONCAMPBELL............................................................... 603

Volume 106 Contents for June Number 3

Taxonomy and EcologyHomology of Ribosomal Ribonucleic Acid of Desulfovibrio Species with Desulfovibrio

vulgaris. B. PACE AND L. LEON CAMPBELL................................... 717Chemical Detection of Microbial Prey by Bacterial Predators. ILAN CHET, SAM FOGEL,

AND RALPH MITCHELL..................................................... 863Resistance of Zygorhynchus Species to Lysis. J.-P. G. BALLESTA AND M. ALEXANDER. .. 938Extrachromosomal Nature of Hydrogen Sulfide Production in Escherichia coli. PORTER

LAYNE, A. S. L. Hu, ALBERT BALOWS, AND BETTY R. DAVIS.................... 1029Starch Gel Electrophoresis of Fructose-6-Phosphate Phosphoketolase in the Genus

Bifidobacterium. V. SCARDOVI, B. SGORBATI, AND G. ZANI..................... 1036

Morphology and UltrastructureCell Wall Composition of the Yeastlike and Mycelial Forms of Blastomyces derma-

titidis. FUMINORI KANETSUNA AND LUIS M. CARBONELL........................ 946Morphology and Ultrastructure of Mycoplasma pneumoniae Spherules. EDWIN S.

BOATMAN AND GEORGE E. KENNY.......................................... 1005Transverse Septum Formation in Budding Cells of the Yeastlike Fungus Candida

albicans. JAMES L. SHANNON AND ALVIN H. ROTHMAN......................... 1026

Genetics and Molecular BiologyInduction of Colicin la at High Temperature. ROSA H. GROMKOVA................ 720Promotor-Like Mutant with Increased Expression of the Glycerol Kinase Operon of

Escherichia coli. MYRA BERMAN-KURTZ, E. C. C. LIN, AND DAVID P. RICHEY.... 724Methionine-Mediated Repression in Saccharomyces cerevisiae: a Pleiotropic Regulatory

System Involving Methionyl Transfer Ribonucleic Acid and the Product of Geneeth2. H. CHEREST, Y. SURDIN-KERJAN, AND H. DE ROBICHON-SZULMAJSTER...... 758

Transduction of R. Factors by a Proteus mirabilis Bacteriophage. RINTARO NAKAYAAND R. ROWND.......................................................... 773

Phenylalanine Biosynthesis in Escherichia coli K-12: Mutants Derepressed for Choris-mate Mutase P-Prephenate Dehydratase. S. W. K. IM AND J. PITTARD.......... 784

Temperature Sensitivity of Maltose Utilization and Lambda Resistance in Escherichiacoli B. A RONEN AND ORINA RAANAN-ASHKENAZI............................ 791

Auxotroph Accumulation in Deoxyribonucleic Acid Polymeraseless Strains of Escher-ichia coli K-12. CLAIRE M. BERG........................................... 797

Effects of pH on Transformation of Bacillus subtilis with Single-Stranded Deoxy-ribonucleic Acid. MARY J. TEVETHIA AND M. MANDEL........................ 802

Relationship Between Competence for Transformation of Bacillus subtilis with Nativeand Single-Stranded Deoxyribonucleic Acid. MARY J. TEVETHIA AND CLAIRE P.CAUDILL................................................................ 808

XiX

CONTENTS

Regulation of Thymidine Metabolism in Escherichia coli K-12: Studies on the Inducerand the Coordinateness of Induction of the Enzymes. ROBERT J. BONNEY ANDHEBERT WEINFELD ........................................................ 812

Transforming Ability of Bacterial Deoxyribonucleic Acid in Relation to the MarkerEfficiencies in Diplococcus pneumoniae During Thymidine Starvation. F. BRUNEL,A. M. SICARD, AND N. SICARD............................................. 904

Competence-Inducing Factor of Bacillus stearothermophilus. ULDIS N. STREIPS ANDN. E. WELKER........................................................... 955

Factors Affecting Transfection in Bacillus stearothermophilus. ULDIS N. STREIPS ANDN. E. WELKER................................ .......................... 960

Minor Threonine Dehydratase Encoded Within the Threonine Synthetic Region ofBacillus subtilis. DANIEL VAPNEK AND SHELDON GREER ....................... 983

Cell Division and Prophage Induction in Escherichia coli: Studies of Nucleotide Levels.W. RUFF, E. P. KIRBY, AND D. A. GOLDTHWAIT.............................. 994

Non-Essentiality of the recA- Mutation in the Phenomenon of Bacteriophage M13-Induced Elimination of F' Factors. S. R. PALCHOUDHURY AND V. N. IYER...... 1040

Difference Between Escherichia coli B/r and Salmonella typhimurium LT-2 in theSedimentation Behavior of the Deoxyribonucleic Acid Synthesized After X-Ir-radiation. TAMARA A. HUDNIK-PLEVNIK AND NADEZDA DJORDJEVIC............ 1043

Attempts to Detect an Alternative Vital Role for the Reduced Nicotinamide AdenineDinucleotide Phosphate-Nitrate Reductase Structural Gene in Aspergillus nidulans.R. J. DOWNEY AND D. J. COVE............................................. 1047

Physiology and MetabolismMutant of Rhodopseudomonas spheroides Unable to Grow Aerobically. T. WITTENBERG

AND W. R. SISTROM....................................................... 732Sporulation in Bacillus brevis: Studies on Protease and Protein Turnover. SAUL SLAPI-

KOFF, JANET. L. SPITZER, AND DENNIS VACCARO............................... 739Transport of Vitamin B12 in Escherichia coli. PAULA M. DI GIROLAMO AND CLIVE

BRADBEER............................................................... 745Isolation of Vitamin B12 Transport Mutants from Escherichia coli. PAULA M. DI

GIROLAMO, ROBERT J. KADNER, AND CLIVE BRADBEER......................... 751Effect of Glutamate on Exogenous Citrate Catabolism of Neisseria meningitidis and

of Other Species of Neisseria. JAMES C. HILL................................ 819Comparison of the Phospholipid Composition of Bifidobacterium and Lactobacillus

Strains. F. A. EXTERKATE, B. J. OTTEN, H. W. WASSENBERG, AND J. H. VEERKAMP. . 824Alkane Oxidation by a Particulate Preparation from Candida. CHAO-MIN-LIU AND

MARVIN J. JOHNSON...................................................... 830Erythromycin-Inducible Resistance in Staphylococcus aureus: Requirements for In-

duction. B. WEISBLUM, C. SIDDHIKOL, C. J. LAI, AND V. DEMOHN.............. 835Isolation and Characterization of Tricarboxylic Acid Cycle Mutants of Bacillus sub-

tilis. R. A. CARLS AND R. S. HANSON....................................... 848Effect of Trimethoprim on Macromolecular Synthesis in Escherichia coli. MARGARET

MIOVIC AND LEWIS I. PIZER............................................... 856Mode of Action of the Competence-Inducing Factor of Bacillus stearothermophilus.

ULDIS N. STREIPS AND FRANK E. YOUNG .................................... 868Fatty Acid Composition of Thermophilic, Mesophilic, and Psychrophilic Clostridia.

MAY CHAN, RICHARD H. HIMES, AND J. M. AKAGI........................... 876Mucopolysaccharide Which Regulates Growth in Neurospora. JosE L. REISSIG AND

JAMES E. GLASGOW......................................................882Dinitrophenol-Stimulated Adenosine Triphosphatase Activity in Extracts of Desulfo-vibrio gigas. LEONARD J. GUARRAIA AND HARRY D. PECK, JR................... 890

Role of Pyridine Nucleotides in 5-Fluorouracil-Mediated Reactivation of UltravioletRadiation Damage. J. M. BOYLE, R. L. SCHENLEY, AND P. A. SWENSON.......... 896

Changes in Hepatic Glycogen, Protein, and Ribonucleic Acid Synthesis, and SomeEffects of Cortisol, During Q Fever. J. STUECKEMANN AND D. PARETSKY........ 920

Repair of Radiation Damage to Deoxyribonucleic Acid in Germinating Spores ofBacillus subtilis. HIROSHI TERANO, HIROSHI TANOOKA, AND HAJIME KADOTA...... 925

Modification of the Composition and Structure of the Yeast Cell Wall by Culture inthe Presence of Sulfur Amino Acids. K. A. KILLICK.......................... 931

3CK

CONTENTS xxi

Variation in the Primary Structure of Bacillus subtilis Flagellins. SUZANNE U. EMERSONAND MELVIN I. SIMON..................................................... 949

Effect of Serine Hydroxamate on the Growth of Escherichia coli. TETSUYA ToSA ANDLEWIS I. PIZER............................... 966

Biochemical Bases for the Antimetabolite Action of L-Serine Hydroxamate. TETSUYAToSA AND LEWIS I. PIZER.................................................. 972

Regulation of Extracellular Protease Production in Bacillus cereus T: Characteriza-tion of Mutants Producing Altered Amounts of Protease. A. I. ARONSON, N.ANGELO, AND S. C. HOLT................................................. 1016

Variability of Photodynamic Killing in Escherichia coli and Avoidance of Variabilitywith Agar. CORLISS O'BRYAN AND ARTHUR P. HARRISON, JR................... 1031

EnzymologyActivities of Tricarboxylic Acid Cycle Enzymes, Glyoxylate Cycle Enzymes, and

Fructose Diphosphatase in Bakers' Yeast During Adaptation to Acetate Oxida-tion. J. P. GOSLING AND P. F. DUGGAN..................................... 908

Production of Phenotypically Epimeraseless Yeast by L-Arabinose. FAROOQ AZAM,SHOU-CHANG Kuo, AND VINCENT P. CIRILLO................................. 915

Isolation and Identification of Trehalase from Pullularia pullulans. EMANUELMERDINGER, CHARLES F. LANGE, AND BEN F. BOOKER......................... 1034