JOURNAL OF BACTERIOLOGYVOLUME 99 * NUMBER 3 * SEPTEMBER 1969

EDITORIAL BOARDL. LEON CAMPBELL, Editor-in-Chief

University of Illinois, UrbanaL. S. BARON, Editor (1970)

Walter Reed Army Institute ofResearchWashington, D.C.

R. D. DeMOSS, Editor (1970)University of Illinois, Urbana

ALLEN G. MARR, Editor (1973)University oj California, DavisERWIN NETER, Editor (1972)

State University ofNew York at Buffalo andChildren's Hospital, Buffalo

Howard I. Adler (1970)R. L. Andron (1969)Robert Ausian (1969)W. Lane Barkadale (1969)S. S. Barkul (1969)Alan W. Behmer (1969)Robert W. Bernlohr (1970)S. Gayled Bradley (1969)Aold F. Brddie (1970)Alan Campbll (1969)George B. Chapman (1969)V. P. CIrillo (1970)Wallace A. Clyde, Jr. (1970)V. W. Cochra (1969)S. F. Coet (1969)C. D. Cox (1969)William J. Cromartie (1969)Roy Curti HI (1969)A. L. Demaln (1969)John A. DeMoss (1969)Micad Doudoroff (1969)Martin Dworkin (1970)Ella Englesberg (1969)Frank B. Engley, Jr. (1969)Stanley Falkow (1969)William W. Ferguson (1969)Leonard Frank (1970)Martin Freundlich (1970)Herman Fridman (1969)Lorraine Friedman (1971)Ruth E. Gordon (1969)Joseph S. Gots (1969)

F. M. Harold (1970)Leonard Hayflick (1969)George Hegeman (1971)James G. Hirsch (1969)D. S. Hoare (1971)John G. Holt (1969)Stanley C. Holt (1971)Paul Howard-Flanders (1969)Milton Huppert (1970)Jerard Hurwitz (1969)John Ingrham (1970)Arthr G. Johnson (1969)R. E. Kallo (1969)Carl Lamanna (1970)Maurice Landy (1970)John H. Law (1971)Edward R. Leadbetter (1970)H. S. Levinson (1969)H. C. I (1970)W. R. L rt (1969)Donald G. Lundgren (1971)Elizabth McCoy (1969)George B. Mackaess (1969)Stanley Marcus (1970)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1969)Frederick C. Neidhardt (1969)M. Nomura (1971)Richard P. Novick (1970)Evelyn L. Oginsky (1969)Kenneth Paigen (1969)

Robert C. Parlett (1969)Harry D. Peck (1970)H. J. Phaff (1969)Jack Preiss (1971)Roy Repaske (1969)H. V. Rickenberg (1970)C. F. Robinow (1969)W. R. Romig (1969)M; R. J. Salton (1969)Moselio Schaechter (1970)Russel W. Schaedler (1969)David Schlessinger (1971)W. R. Sistrom (1969)Paul F. Smith (1970)Roger Y. Stanier (1970)M. P. Starr (1970)B. A. D. Stocker (1969)Bernard S. Strauss (1969)Paul S. Sypherd (1971)A. L. Taylor (1971)Curtis B. Thorne (1969)H. E. Umbarger (1970)Lewis W. Wanamaker (1969)Arthur Weissbach (1971)N. E. Welker (1970)R. W. Wheat (1969)Helen R. Whteley (1969)J. B. Wilson (1969)Meyer J. Wolin (1970)Willis A. Wood (1969)Barbara E. Wright (1971)Frank E. Young (1970)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIOE. M. FOSTER, President (1969-1970)

DONALD E. SHAY, SecretaryThe Journal of Bacteriology, a publication of the American

Society for Microbiology, 4715 Cordell Ave., Bethesda, Md.20014, is devoted to the advancement and dissemination of funda-mental knowledge concerning bacteria and other microorganisms.The Journal is published monthly, and the twelve numbers aredivided into four volumes per year. The subscription price is$40.00 (Postal Union Countries, $42; other foreign, $43) peryear, including Bacteriological Reviews. Single copies are $4.00(foreign, $4.25). Members of the American Society for Micro-biology may receive the Journal as part of their dues. Correspond-ence relating to reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts,and general editorial matters should be directed to the ASMPublications Office, 4715 Cordell Ave., Bethesda, Md. 20014.

R. E. HUNGATE, Vice-President (1969-1970)H. B. WOODRUFF, Treasurer

Correspondence from ASM members relating to membershipdues, member subscriptions, changes of address, incorrect address,incorrect journals, etc., should be directed to the American So-ciety for Microbiology, 1913 I St., N.W., Washington, D.C.20006. Nonmembers of the Society should write to The Williams& Wilkins Co., 428 E. Preston St., Baltimore, Md. 21202, regard-ing institution of nonmember subscriptions and nonreceipt ofjournals. Published monthly by the ASM at 428 E. Preston St.,Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at addi-tional mailing offices.Made in the United States of America.Copyright a 1969, American Society for Microbiology.All Rights Reserved

1

Author IndexAndersen, R. N., 867Anderson, Kenneth E., 893Andreoli, A. J., 895

Babcock, K. L., 650Bacon, J. S. D., 885Beaman, B. L., 876Becker, George E., 891Bishop, Helen L., 771Bradley, S. G., 702

Chang, Y. T., 867Chang, Yung Feng, 667Cohen, G. N., 791Cohen, Jay O., 737Copeland, James C., 730Cruces, Griselda, 702

Dawson, G. W. P., 779Deibel, R. H., 674DeMoss, J. A., 720Doi, Roy H., 771

Fangman, Walton L., 681Feeley, John C., 645Feingold, David Sidney, 667Flinton, Linda, 908Fortnagel, Uta, 745Foulds, John D., 655, 661Frank, Hilmer A., 824Freese, Ernst, 745

Galun, Esra, 802Germano, Geno J., 893Gerone, Peter J., 910Gholson, R. K., 895Gibbons, R. J., 888

Green, M. L., 834Greenberg, Bernard, 629Guyer, Mark, 906

Hamkalo, Barbara A., 815Happold, F. C., 834Hartwell, Leland H., 807Hatch, George G., 902Hegeman, George, 906Hohl, Hans R., 824Hutchison, H. Terry, 807

Inouye, Masayori, 842

Jones, D., 885Jyssum, Kaare, 757

Kelstrup, J., 888Kurzepa, Henryka, 908Kwok, S. C., 897

Le Bras, Gisele, 791

McDade, Joseph E., 910McLaughlin, Calvin S., 807Matney, Thomas S., 895Metcalf, Robert H., 674Meyer, K. F., 636Migita, Lloyd K., 771Miller, Carl E., 688Monson, A. M., 702

Nagel de Zwaig, Rosa, 913

Ottolenghi, P., 885

Pai, C. H., 696Panek, Anita D., 904Peterson, Anne M., 639

Pittard, J., 707Pollock, Mary E., 639

Reed, Norman D., 902Reichenbach, Hans, 856Ronen, A., 784Ruiz-Herrera, Jose, 720, 764Rusch, H. P., 650

Sack, R. Bradley, 688Saito, Hajime, 851Santo, Leatrice Miura, 824Sasek, Vaclav, 891Sauer, H. W., 650Schachter, Julius, 636Shankel, D. M., 876Shemin, David, 655, 661Stakebake, Jack R., 910Stanier, R. Y., 791Starkey, Robert L., 764Stewart, Ronald H., 899Stuttard, Colin, 779Suzuki, Isamu, 897Swenson, P. A., 815

Tasaka, Hiromichi, 851Thompson, J., 834Tritz, Gerald J., 895

VanDemark, P. J., 908Voelz, Herbert, 856

Walker, James, R., 713Wallace, B. J., 707Weinman-Greenshpan, Dvora, 802Wright, D. N., 899

Zehavi, A., 784

JOURNAL OF BACTERIOLOGY

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INDEX TO DATE OF ISSUE

Month Date of Issue Pages

July 7/25/69 1-360August 8/25/69 361-628September 9/24/69 629-916

AUTHOR INDEX

Volume 99

Acton, Ronald T., 626Adler, H. I., 621Ahmed, S. I., 231Akagi, J. M., 210Aleem, M. I. H., 142Alred, Elizabeth N., IAndersen, R. N., 867Anderson, Kenneth E., 893Andreoli, A. J., 895Apirion, David, 206Arst, Herbert N., Jr., 113

Babcock, K. L., 650Bacon, J. S. D., 885Bailey, G. D., 491Baptist, James N., 180Barkley, D. S., 510Barnes, Isabel J., 169Baron, L. S., 242, 358Beaman, B. L., 876Becker, George E., 891Bekker, J. H., 132Berg, Paul, 274Berliner, Martha D., 1Berlyn, Mary B., 222Biggins, John, 570Bishop, Helen L., 771Bleyman, Michael, 535Blumer, M., 366Bondi, Amedeo, 169Bonner, J. T., 510Bradley, S. G., 702Braun, Werner, 125Breitman, T. R., 336Brown, C. M., 371Brownell, George H., 25Burns, R. 0., 91Burris, Carroll, 341

Cameron, C., 57Chang, Y. T., 867Chang, Y. Y., 510Chang, Yung Feng, 667Chapman, G. B., 326Chase, T., 366Cohen, G. N., 791Cohen, Jay O., 737Colwell, R. R., 326Copeland, James C., 730Cove, David J., 113Cox, G. B., 450Cruces, Griselda, 702Cummings, Donald J., 558

Dalal, Fram R., 441Danielsson, Dan G., 469Dawson, G. W. P., 779DeAngelo, Anthony B., 463

DeBruijn, J. H., 132Dehority, B. A., 189Deibel, R. H., 674Demain, Arnold L., 347DeMoss, J. A., 720Doi, Roy H., 771Douglas, H. C., 287Duntze, Wolfgang, 590

Edwards, John R., 495Elliker, P. R., 603Elorza, M. Victoria, 113Epstein, Israel, 414, 418Erbstoeszer, John W., 151Erickson, Robert J., 125Esposito, Vito M., 8, 13, 356Evans, E. Edward, 626

Fangman, Walton L., 681Feeley, John C., 8, 13, 645Feingold, David Sidney, 667Felter, R. A., 326Finkleman, A., 336Fisher, W. D., 621Flinton, Linda, 908Forman, Arnold, 576Forsyth, Goerge W., 269Fortnagel, Uta, 745Foulds, John D., 661, 665Fralick, J. A., 621Frank, Hilmer A., 824Freese, Ernst, 745Freundlich, Martin, 101, 107Frost, G. M., 395

Galun, Esra, 802Germano, Geno J., 893Gerone, Peter J., 910Gholson, R. K., 895Gibbons, R. J., 888Gibson, F., 450Giles, Norman H., 221, 231Goldberg, L. J., 491Gots, Joseph S., 441Gough, Michael, 350Grant, Geoffrey F., 116Green, I., 434Green, M. L., 834Greenberg, Bernard, 629Grindal, D. Paul, 287Grossowicz, Nathan, 414, 418Gruber, Jack, 18Guyer, Mark, 906

Hamkalo, Barbara A., 815Hane, Michael W., 238Happold, F. C., 834Hartwell, Leland H., 807

Hatch, George G., 902Hatten, Betty A., 611Hecht, Norman, 535Hegeman, George D., 353, 906Hengstenberg, Wolfgang, 383Henney, Henry R., Jr., 531Hildemann, W. H., 626Hill, C. W., 274Hill, Katherine L., 383Hiraga, Sota, 279Hohl, Hans R., 824Holme, T., 513Hsieh, Hsi-chia, 406Huang, Shyi Yi, 611Hubbard, Jerry S., 161Hutchison, H. Terry, 807

Inouye, Masayori, 842Ito, Koreaki, 279

Jacobs, J. M., 37Jacobs, N. J., 37Jannasch, Holger W., 156Janosko, Nancy, 590Jarolmen, H., 487Johnson, E. M., 358Jones, D., 885Jones, H. C., 316Jones, Evan E., 269Jyssum, Kaare, 263, 757

Kane, Judith, 175Karakawa, W. W., 175Kelley, Karol L., 25Kelstrup, J., 888Kemp, G., 487Khouw, Boen Tie, 197Kondo, Masatoshi, 535Konijn, Theo M., 503, 510Korczynski, M. S., 552Krause, R. M., 175Kundsin, Ruth B., 1Kurzepa, Henryka, 908Kusy, Alvin R., 558Kwok, S. C., 897

Lackland, Henry, 175Lago, Barbara D., 347Landman, Otto E., 576Leahey, J. E., 527Le Bras, Gisele, 791Lee, Linda, 469Leeder, Wayne D., 8, 13Lehrer, R. 1., 361Lindberg, A. A., 513Lippincott, Barbara B., 496Lippincott, James A., 496Liu, Pinghui V., 406

AUTHOR INDEX

Livingston, Laura R., 85Lockwood, L. B., 623Loesche, Walter J., 520Loomis, William F., Jr., 65Lundgren, D. G., 552Luria, S. E., 78Luzzatto, Lucio, 206Lynch, Thomas, 531

McCann, L. M., 450McCarthy, Brian J., 248, 255McCurdy, Howard D., 197McDade, Joseph E., 910McKay, L. L., 603McLaughlin, Calvin S., 807Malamy, A., 422, 434Mandel, Manley, 180Manney, Thomas R., 590Matney, Thomas S., 895Maverakis, Nick H., 472Metcalf, Robert H., 674Meyer, K. F., 636Migita, Lloyd K., 771Miller, Alan B., 161Miller, Ann L., 401Miller, Carl E., 688Miller, James N., 132Milner, Kelsey C., 51Moat, Albert G., 169Moore, Richard L., 248, 255Monson, A. M., 702Morse, M. L., 383, 567Munoz, J., 57

Nagel de Zwaig, Rosa, 78, 913Norcross, N. L., 527

O'Brien, R. W., 389, 395O'Neil, Dennis M., 242Onvlee, P. C., 132Op den Kamp, J. A. F., 298, 304Ottolenghi, P., 885

Pai, C. H., 696Panek, Anita D., 904Park, James T., 495Parsons, S. J., 91Peacock, William L., Jr., 469Penberthy, W. K., 383Perry, Jerome J., 216Peterson, Anne M., 639Pfennig, Norbert, 597, 619Pittard, J., 707Pittman, Margaret, 8

Pollock, Beatrice F., 567Pollock, Mary E., 639Prestidge, Louise, 70

Rabinovitz, M., 336Raper, John R., 291Reagan, R. L., 336Redai, I., 298Reed, Norman D., 902Reichenbach, Hans, 856Ribi, Edgar, 51Ronen, A., 784Rose, A. H., 371Rosenberg, S. L., 353Ross, R., 57Roth, I. L., 316Rudbach, Jon A., 51Ruiz-Herrera, Jose, 544, 720,

764Rusch, H. P., 650

Sack, R. Bradley, 688Saito, Hajime, 851Salazar, Jorge, 590Sanders, W. M., III, 316Sandine, W. E., 603Santo, Leatrice Miura, 824Sagek, Vaclav, 891Sauer, H. W., 650Scazzocchio, Claudio, 113Schachter, Julius, 636Schaefler, S., 422, 434Schiffer, Dinah, 274Schlessinger, David, 206Schmale, John D., 469Schnaitman, C. A., 552Schulze, Mary L., 611Shankel, D. M., 876Shaw, Charles R., 180Shaw, Paul D., 463Shemin, David, 655, 661Sheng, G. S., 37Shilo, M., 136Shimodori, Shoichi, 339Short, Steven A., 142Shumas, Susan R., 441Siddhikol, Chalongkwan, 151Simon, Melvin I., 116Smith, Joseph F., 469Smith, Paul F., 480Spizizen, John, 70Stakebake, Jack R., 910Stanier, R. Y., 791Stark, D. M., 527Starkey, Robert L., 544, 764

Stern, Joseph R., 389, 395Sternberg, M., 623Stewart, Ronald H., 899Streips, Uldis N., 344Stuttard, Colin, 779Suh, Byungse, 210Sulkin, S. Edward, 611Suzuki, Isamu, 897Swenson, P. A., 815Sypherd, Paul S., 242, 379

Takeya, Kenji, 339Talbott, Helen, 287Tasaka, Hiromichi, 851Theil, Elizabeth C., 269Thompson, J., 834Trela, John M., 101, 107Tritz, Gerald J., 895Tucci, Anthony F., 624

van Deenen, L. L. M., 298VanDemark, P. J., 908van de Meene, J. G. C., 510van Iterson, W., 304Varon, Mazal, 136Vestal, J. R., 216Voelz, Herbert, 856

Walker, James B., 401Walker, James R., 713Wallace, B. J., 707Walter, L. A., 603Wang, Chiu-Sheng, 291Watson, S. W., 366Weinheimer, Peter F., 626Weinman-Greensphan, Dvora,802

Weisblum, Bernard, 151Welker, N. E., 344White, David C., 142Wiley, Bill B., 472Williams, Carol O., 341Wittler, Ruth G., 341Woese, Carl, 535Wood, Thomas H., 238Wright, D. N., 491, 899Wright, George C., 18

Youmans, Anne S., 42Youmans, Guy P., 42Young, I. G., 450Young, Frank E., 125Yura, Takashi, 279

Zehavi, A., 784

* 7

J. BACTERIOL.

SUBJECT INDEX

Volume 99

Acid-fast organisms-nonsolid; viability of, 867Adenosine-3', 5-monophosphate-bacterial attractant for myxamoebae, 510Adenosine triphosphate-inhibition of yeast trehalase, 904Aerobacter aerogenes-aerobic citrate catabolism in, 389Aerobic citrate catabolism-in A. aerogenes, 389-role of sodium, 389Aerosols-of M. pneumonia, 491Agar-free liquid medium-adaptation of meningococcal L-forms to, 899Agar media-chemically defined and complex, growth of No-

cardia on, 876Agar plaque formation-by mouse spleen cells, 356Agrobacterium-complementation in, 496Agrobacterium tumefacienis-hexuronic acid dehydrogenase of, 667Algae-blue-green, respiration in, 570Amino acid-antagonist death in E. coli, 336Amino acid starvation-effect on regulation of chromosome replication in

B. subtilis, 730Amino acids-of the Aspartate family, 791Ammonia-oxidation by spheroplasts of Nitrosomonias europaea,

897Ammonium ion-in the biosynthesis of ,#-nitropropionic acid, 463Ammonium salts-effect on protease production of bacteria, 406Ammonium sulfate coprecipitation-in study of enterotoxins of S. aureus, 18Anacystis nidulans-endogenous respiration, 570Anaerobic metabolism-of benzoate by Rhodopseudomonas palustris, 906Anerobic metabolism of benzoate-evidence for a reductive pathway, 906Anions-lytic response of enterococci associated with, 674Anthranilic acid-requirement for in Salmonella typhimurium, 779Antibody-protective, of S. dysgalactiae, 527Antifungal effects-of peroxidase systems, 361Antigenic relationship

-between Brucella species and Vibrio cholerae, 645Antigens-of Bordetella, 57-of T. pallidum, 132Aquatic bacteria-growth rates, 156Arginine regulon-of E. coli, 269Aromatic vitamins-biosynthesis of, 707Aspartate family-amino acids of, 791Aspergillus nidulans-permeability properties of protoplasts, 113-protoplasts, 113Aspergillus sp.-dissimilation of methionine by a demethiolase of,764

Bacillus-phospho-,1-glucosidases and,-glucosidase permease

in, 434-prototrophic thermophilic, 414Bacillus licheniformis-transformation system, 70Bacillus stearothermophilus-infection with bacteriophage DNA, 344Bacillus subtilis-binding of gamma globulin, 125-gelatin-induced reversion of protoplasts, 576-gymnoplasts of, 304-mutants blocked in the pyruvate dehydrogenase

complex, 745-phospholipid composition, 298-protoplasts of, 304-regulation of chromosome replication, 730-transduction of flagella-specific markers, 116Bacillus subtilis extracts-peptide synthesis by, 771Bacillus subtilis mutants-growth and sporulation of, 745Bacillus subtilis spores-peptide synthesis by extracts from, 771Bacteria-effect of ammonium salts on protease production of,406

-effect on chemotaxis in cellular slime molds, 503-separability in, 222Bacterial contamination-effect on cecal size and cecal contents of gnotobiotic

rodents, 520Bacterial flagella-synthesis of, 116Bactericidal response-induced, in the hagfish, 626Bactericidins-synthesis by hagfish, 626

SUBJECT INDEX

Bacteriocin-from Serratia marcescens, 655-inhibition of E. coli growth by, 655-synthesis of, 661Bacteriocin inactivator-synthesis of, 661Bacteriocins-in the intestinal canal and oral cavity, 888-inactivation of, 888Bacteriophage Felix 0-1-influence of 0 side chains on, 513Bacteriophage X-resistant-mutants of Escherichia coli, 784Basal media-for Myocplasma culture, 908Bdellovibrio bacteriovorus-interaction with host bacteria, 136-intracellular growth, 136Bedsonia group-causative agent of lymphogranuloma venereum,

636Benzoate-anaerobic metabolism by Rhodopseudomonas palus-

tris, 906Binding-of gamma globulin in B. subtilis, 125Biosynthesis-of amino acids of the aspartate family, 791-of aromatic vitamins, 707-of desthiobiotin in cell-free extracts of E. coli, 696-of glycolipids, 480-of ,B-nitropropionic acid, 463-serine, in Desulfovibrio desulfuricans, 893Blastociadiella emersonii-morphogenesis in, 197-tricarboxylic acid cycle in, 197Blue-green algae-respiration in, 570Bordetella-antigens of, 57-histamine-sensitizing factor, 57Bovine rumen-pectin-fermenting bacteria from, 189Branched-chain amino acid enzymes-repression of, 101Brevibacterium species-metabolism of propane and propionate, 216Brucella-somatic 0 antigen relationship to Vibrio cholerae,

645Brucella abortus-L-forms, 611Budding purple nonsulfur bacteria-R. acidophila, a new species, 597

Calliphora vicina-(blowfly), Salmonella suppression by bacteria in,

629Cantdida utilis-fatty acids in, 371Capsular types-multiplicity in S. aureus, 472Capsules-serologically distinct in S. aureus, 472

Cation-activated-nucleotidase, 834Cecum-size and contents of gnotobiotic rodents, 520Cell division-unlinking from DNA replication, 842Cell-free extracts-of E. coli, 696Cell growth-of Nocardia on chemically defined and complex

agar media, 876Cellular slime molds-effect of bacteria on chemotaxis, 503Cephalosporins-binding versus growth inhibition, 459Chain termination-in polarity mutants, 91-premature, effect on fidelity of initiation, 91Chemotaxis-in cellular slime molds, 503Chloramphenicol-effect on mitomycin C inactivation in E. coli, 558-effect on nalidixic acid inactivation in E. coli, 558-effect on thymineless death in E. coli, 558-effect on UV irradiation in E. coli, 558Chlorazol black E-staining of L-forms, I-staining of Mycoplasma, ICholera-observations incident to the study of, 688Chromosome replication-in B. subtilis, 730-in N. meningitidis, 757Citrate catabolism-in S. typhimurium, 395Colicin A-effect--on energy-requiring cellular functions of E.

coli, 913-mode of action, 913Colicin-tolerant mutants-of E. coli; a new class, 78Colicins-inactivation of in the intestinal canal and oral

cavity, 888Coliform bacteria-biosynthesis of amino acids of the aspartate family

in, 791Common aromatic synthetic pathway-organization of enzymes in, 231Competence-in the B. lichenziformis transformation system, 70Complementation-in Trichoderma, 802-of tumor growth in Agrobacterium, 496Coprecipitation-with purified enterotoxins, 18Culture medium composition-effect on production ofM protein and proteinase bygroup A streptococci, 737

Cytochrome bi-participation in nitrate reduction, 720

Demethiolase-of Aspergillus sp., 764

iV J. BACTERIOL.

SUBJECT INDEX

3-Deoxy-D-arabino-heptulosonic acid 7-phosphatesynthetase activity

-regulation of, 707Deoxyribonucleic acid-base sequence homology, 255-from bacteriophage TP-1C, 344-homologies in Mycoplasma, 639-in halophilic bacteria, 248-infection of B. stearothermophilus, 344-of extremely halophilic bacteria, 255-renaturation studies, 255-replication, unlinking of cell division from, 842Deoxyribonucleic acid replication-unlinking from cell division, 842Deoxyribonucleic base compositions-of Mycoplasma species and L-phase variants, 341Desthiobiotin-biosynthesis of, 696Desulfovibrio desulfuricans-serine biosynthesis in, 893Desulfovibrio vulgaris-formation of thiosulfate from sulfite by, 210Dictyostelium discoideum-bacterial attractant for myxamoebae of, 510-mutants (temperature-sensitive) of, 65Dissimilation-of methionine, 544-of methionine by a demethiolase of Aspergillus sp.,

764Dissolved-oxygen tension-effect on fatty acids in C. utilis, 371Drug-resistant bacteria-transfer in mixed culture, 487

Electrophoretic mobilities-in starch gels of enzymes of Enterobacteriaceae, 180Endotoxin-in bacterial protoplasm, 51Enterobacteriaceae-phospho-,B-glucosidases in, 422Enterococci-lysis of, 674Enzymes-aggregation in fungi, 231-organization in the polyaromatic synthetic pathway,

222Escherichia coli-auxotrophs of killed by analogues of amino acids,

336-B. bacteriovorus growing on, 136-bacteriophage X-resistant mutants, 784-cell-free extracts of in the synthesis of desthiobiotin

696-colicin-tolerant mutants; a new class, 78-duplication of genetic material, 274-effect of colicin A, 913-inhibition of growth by bacteriocin from Serratia

marcescens, 655-intergeneric mating with S. typhosa, 242-lacking thymidine phosphorylase, specificity and

efficiency of thymidine incorporation in, 681-nitrate reductase complex of, 720-radiation sensitivity, 713-reversion instability in the galactose operon, 567

-30S ribosomal protein from, 379-ribosome function, 205-temperature-sensitive DNA synthesis mutant of,

842-temperature-sensitive repression of the tryptophan

operon, 279-thymineless death in, 558-utilization of NAD, 895Escherichia coli B-effects of UV irradiation on growth and respiration,

815-radiation-resistant and radiation-sensitive strains

of, 815Escherichia coli K-I 2-biosynthesis of ubiquinone in, 450-genetic mapping and dominance studies, 238-mutants resistant to naladixic acid, 238-regulation of DAHP synthetase activity in relation

to the synthesis of aromatic vitamins in, 707Escherichia coli ras locus-its involvement in radiation repair, 713Escherichia coli W-arginine regulon of, 269-M gene, a regulatory gene, 269Estradiol-wasting syndrome induced in mice by, 902Estradiol-induced wasting syndrome-pathogenesis of, 902

Fatty acids-effect of growth temperature and dissolved-oxygen

tension, 371-in C. utilis, 371-in lipids of nitrifying bacteria, 366Feedback inhibition-of lysine biosynthesis in yeast, 624Ferrobacillus ferrooxidans-iron oxidation by, 552-lipid composition, 142Flagella markers-transduction of in B. subtilis, 1 16Formate dehydrogenase-participation in nitrate reduction, 720Fruiting bodies-of Stigmatella aurantiaca (Myxobacterales), 856Fungi-aggregation of enzymes in, 231-dissimilation of methionine by, 544

Galactose operon-of E. coli, reversion instability, 567Galactosides-in phosphotransferase system of S. aureus, 383Gamma globulin-binding by B. subtilis cultures, 125Gelatin-effect on protoplasts of B. subtilis, 576Genes-functionally related, in P. aerugilnosa, 353Genetic factor determining competence-influence on origin and sequence of chromosome

replication in Neisseria meningitidis, 757Genetic homologies-among strains of Streptomyces violaceoruber, 702

VOL. 99, 1969 v

SUBJECT INDEX

Genetic transfer-of Vi antigen, 358Genome size-relative, in Mycoplasma, 639j3-Glucosidase permeases-in Streptococcus, Bacillus, and Staphylococcus, 434Glucosyl diglycerides-biosynthesis by M. laidlawii, 480Glycolipids-biosynthesis, 480Gnotobiotic rodents-cecal size and cecal contents, 520Gonococcal antigens-isolation of, 469Gymnoplasts (protoplasts)-of B. subtilis, 304

Hagfish-induced bactericidal response, 626Halobacterium-DNA-containing bacteria, 248Halobacterium cutirubrum-NADP-specific isocitrate dehydrogenase of, 161Halococcus-DNA-containing bacteria, 248Halophilic bacteria-satellite DNA in, 248Heme-accumulation of, 37Hexuronic acid dehydrogenase-formation of, 667-of Agrobacterium tumefacienis, 667Human immunity-to gonococcal infection, 469

Immunity-to Vi antigen, 13Immunogenic activity-of myocbacterial ribosomal preparations, 42-of RNA preparations, 42In vivo-transmission of R factors, 487Inactivation-of the isocitrate dehydrogenase from an obligate

halophile, 161Initiation-of protein synthesis after premature chain termina-

tion in polarity mutants, 91Inosine-5'-monophosphate cyclohydrolase-transfor-

mylase complex-of S. typhimurium, 441Inosinic acid cyclohydrolase-transformylase complex

of S. typhimurium-genetic separation of, 441Intergeneric bacterial mating-between E. coli and S. typhosa, 242Iron oxidation-by F. ferrooxidans, 552Isocitrate dehydrogenase-from an obligate halophile, 161-purification and reversible inactivation of, 161Isomaltose-oxidation by P. taetrolens, 623

Kinetics-of iron oxidation, 552

L-forms-of B. abortus, 611L-forms-of N. meningitidis, adaptation to an agar-free liquidmedium, 899

-penicillin-induced in B. abortus, 611-staining with Chlorazol Black E, I-ultrastructure of, 611L-phase variants-DNA base compositions of, 341Lactose-utilization by group N streptococci, 603Leakiness-of X-resistant mutants of Escherichia coli, 784Lethal factor-in vibrios, a new detection method, 339Lymphogranuloma venereum-characterization, 636Lysine-biosynthesis in yeast, 624Lysine auxotrophs-biochemical characterization, 169-of S. aureus, 169Lysine biosynthesis-feedback inhibition, 624Lysozyme-lytic response of enterococci associated with, 674Lytic response-of enterococci associated with addition order of

lysozyme and anions, 674

M protein-production by group A streptococci, 737Macromolecule synthesis-in protoplast reversion, 576Marine bacteria-cation-activated nucleotidase in cell envelopes of,

834-fatty acids in, 366Mating factors-in nocardial recombinants, 25Meningococcal L-forms-adaptation to agar-free liquid medium, 899Merodiploidy-transduction of induced duplication of recipient

genes, 274Methionine-dissimilation, 544-dissimilation by a demethiolase of Aspergillus sp.,

764-requirement for in mutants of P. denitrificans, 347Minicell extracts-polyuridylic acid-directed phenylalanine incorpora-

tion in, 621Miyagawanella lymphogranulomatosis-recently isolated strains, 636Morphogenesis-in B. emersonii, 197Morphological changes-of Mycobacterium lepraemurium grown in cultures

of mouse peritoneal macrophages, 867

Vi J. BACTERIOL.

SUBJECT INDEX

Mouse peritoneal macrophages-morphological changes in Mycobacterium leprae-murium grown in, 867

Mouse strains-response to typhoid vaccine and Vi antigen, 8Mucopeptide-from S. boris, 175Mutant bacteria-thymidine incorporation in, 681Mutants-of B. subtilis, 745-of D. discoideum, 65-of E. coli; pleiotropic maltose-negative, X-resistant,

784-of Trichoderma, 802Mycobacterial ribosomal preparations-factors affecting immunogenic activity of, 42Mycobacterium abscessus-and M. fortuitum, a comparison of pathogenicity,

851Mycobacterium Jortuitum-and M. abscessus, a comparison of pathogenicity,

851Mycobacterium lepraemurium-morphological changes when grown in cultures ofmouse peritoneal macrophages, 867

Mycoplasma-parasitic; growth without serum or serum fraction,

908-staining with Chlorazol Black E, IMycoplasma hominis-DNA homology, 639Mycoplasma laidlawli-biosynthesis of glucosyl diglycerides, 480-types A and B, DNA homology, 639Mycoplasma pneumonia-airborne, effect of temperature, 491Mycoplasma pulmoniis-DNA homology, 639Mycoplasmas-DNA base compositions of, 341Myeloperoxidase-candidacidal activity, 361-human, lethality, 361Myxamoebae-bacterial attractant for, 510Myxobacterales-Stigmatella aurantiaca, fruiting bodies of, 856Myxomycete-defined medium, 531

Naladixic acid-E. coli mutants resistant to, 238Natural waters-bacterial growth rates, 156Neisseria golnorrhoeae-antigen in the human response to gonococcal in-

fection, 469Neisseria meningitidis-chromosome replication in, 757-entry of transforming markers after chromosome

alignment, 263Neisseria meningitidis L-forms-adaptation to an agar-free liquid medium, 899

Neurospora crassa-cell wall composition in osmotic mutants and wild

type, 85-osmotic mutants of, 85Nicotinamide adenine dinucleotide-utilization by E. coli, 895Nitrate reductase complex-of E. coli, 720Nitrate reduction-participation of specific formate dehydrogenase andcytochrome b1 , 720

Nitrifying bacteria-fatty acids in lipids of, 366Nitrogen sources-effect on cellular form of Trigontopsis variabilis, 891-effect on triangularity of cells, 891,B-Nitropropionic acid-biosynthesis of, 463Nitrosomonas europaea-oxidation of ammonia by spheroplasts of, 897Nocardia-mating factors, 25Nocardia cell growth-ultrastructure of, 876Nocardial recombinants-inheritance of mating factors in, 25Nonconidiating mutants-of Trichoderma, 802Nonsolid acid-fast organisms-viability of, 867Nucleotidase-cation-activated in cell envelopes of a marine bac-

terium, 834Nutritional requirements-of trpA gene mutants of Salmonella typhimiurium,

779

O side chains-influence of, 5133-Octaprenyl-4-hydroxybenzoic acid-genes affecting metabolism, 4502-Octaprenylphenol-genes affecting metabolism, 450Osmotic mutants-of N. crassa, 85Oxidation-of isomaltose by P. taetrolens, 623Oxygen-effect on heme and porphyrin accumulation, 37

Parasitic Mycoplasma-growth without serum or serum fraction, 908Pathogenicity-for mice of Mycobacterium Jortuitum and M. ab-

scessus, 851Penicillin-induced-L-forms of B. abortus, 611Penicillins-binding versus growth inhibition, 459Peptide synthesis-by extracts from B. subtilis spores, 771Peroxidase systems-antifungal effects, 361pH

* -

VOL. 99, 1969

SUBJECT INDEX

-effect on production of M protein and proteinaseby group A streptococci, 737

Phenylalanine incorporation-polyuridylic acid-directed, 621Phormidium luridum-endogenous respiration, 570Phospho-,3-glucosidases-expressed and cryptic, 422-in Enterobacteriaceae, 422-in Streptococcus, Bacillus, and Staphylococcus, 434Phosphoenolpyruvate-in lactose utilization, 603Phosphoglucomutase-electrophoretic variants of, 287-in Saccharomyces sp., 287Phospholipids-in B. subtilis, 298-metabolism in F. ferrooxidanis, 142Phosphorus compound-in extracts of slime mold, 650Phosphorylation-of streptomycin, 401Phosphotransferase system-of S. aureus, 383-requirement for galactosides, 383Physarum flavicomum-growth in chemically defined medium, 531Physarum polycephalum-polyphosphate in, 650Physarum rigidum-growth in chemically defined medium, 531Plaque assay system-for species of rickettsiae, 910Pollution control-function of slime layers, 316Polyaromatic synthetic pathway-organization of enzymes in, 222Polyphosphate-in Physarum polycephalum, 650Polyuridylic acid-effect on phenylalanine incorporation in minicell

extracts, 621Porphyrins-accumulation of, 37Propane-metabolism, 216Propionate-metabolism, 216Protease-production of bacteria, inhibition of, 406Protective antibody-of S. dysgalactiae, 527Protein breakdown-intracellular, in a thermophile, 418Protein specificity-in S. communie, 291Protein synthesis-fidelity of initiation, 91-in S. typhimurium, 107Proteinase-production by group A streptococci, 737Proteus mirabilis-survival in the blow fly, 629Protoplasm

-bacterial endotoxin in, 51-from gram-negative bacteria, 51Protoplasts-of A. nidutlanis, 113-of B. subtilis, gelatin-induced reversion, 576-permeability properties, 113Pseudomonads-biosynthesis of amino acids of the aspartate family

in, 791Pseudomonias aeruginosa-clustering functionally related genes, 353-survival in the blow fly, 629Pseudomonas denitrificanis-Vitamin B12- and methionine-requiring mutants, 347Pseudomonias taetrolens-oxidation of isomaltose, 623Purple nonsulfur bacteria-R. tenue, a new species, 619Putrefactive anaerobe 3679h-sporulation of, 824-ultrastructure during sporulation, 824Pyruvate dehydrogenase complex-growth and sporulation of B. subtilis mutants

blocked in, 745

R factor-transmission in vivo, 487Radiation repair-involvement of the E. coli ras locus, 713Radiation-resistant-strains of E. coli B, 815Radiation-sensitive-strains of E. coli B, 815Radiation sensitivity-of E. coli, 713Reductive pathway-for the anaerobic metabolism of benzoate, 906Regulation-of chromosome replication in B. subtilis, 730Reversion instability-in E. coli, 567Rhodopseudomolnas acidophila-budding purple nonsulfur bacteria, 597-new species, 597Rhodospirillum teniue-new species, 619Purple nonsulfur bacteria, 619Ribonucleic acid-yeast mutant defective in production of, 807Ribonucleic acid polymurase-effect of streptolydigin and rifamycin, 151Ribonucleic acid preparations-factors affecting immunogenic activity of, 42Ribonucleic acid synthesis-S. typhimurium, 107Ribosomal cistrons-organization of in B. subtilis genome, 535Ribosomal protein-amino acid differences in from two strains of E. coli,

379-30S, from E. coli, 379Ribosomal protein cistrons-chromosomal location of, 242Ribosome function

. .i. J. BACTERIOL.

SUBJECT INDEX

-streptomycin-inhibition, 205Rickettsia rickettsii-plaque assay system for the bitter root strain, 910Rickettsiae-plaque assay system for several species, 910Rifamycin-effect on RNA polymurase, 151Round bodies-of Vibiro, 326Rumen bacteria-pectin-fermenting, 189

Saccharomyces sp.-phosphoglucomutase in, 287Saccharomyces cerevisiae-temperature-sensitive growth response in a mutant

of, 807-tryptophan synthetase mutants, 590Salmonella bacteria-influence of 0 side chains on attachment of FOphage to, 513

Salmonella sp.-suppression by bacteria in flies, 629Salmonella typhimurium-effect of 5',5',5'-trifluoroleucine on repression, 101-effect of 5',5',5'-trifluoroleucine on uncoupling of

protein and RNA synthesis, 107-inosine-5'-monophosphate cyclohydrolase-transfor-

mylase complex of, 441-role of sodium in growth and citrate catabolism, 395-self-feeding strain, 779-survival in the blow fly, 629-thermal reversal of UV damage, 350Salmonella typhosa-intergeneric mating with E. coli, 242Schizophyllum commune-protein specificity in, 291-sexual morphogenesis in, 291Self-feeding strain-of Salmonella typhimuriunm, 779Serine-biosynthesis in Desulfovibrio desuljiiricalns, 893Serotypes-of vibrios, 688Serratia marcescenis-bacteriocin from, 655-concomitant synthesis of bacteriocin and bacteriocin

inactivator from, 661Sexual morphogenesis-in S. commune, 291Slime layers-electron microscopic study, 316-function in pollution control, 316Slime mold-polyphosphate in, 650Snail digestive juice-effect on yeast cells, 885Sodium-in growth and citrate catabolism, 395-regulation of aerobic citrate catabolism, 389Somatic 0 antigen-relationship of Brucella and Vibrio cholerae, 645Spheroplasts

-of Nitrosomo,ias europaea, oxidation of ammonia,897

Sporulation-of putrefactive anaerobe 3679h, 824Staphylococcus-phospho-,B-glucosidases and,3-glucosidase permease

in, 434Staphylococcus aureus-ammonium sulfate coprecipitation technique ap-

plied to, 18-growth inhibition and binding of penicillins and

cephalosporins, 459-lysine auxotrophs of, 169-multiplicity of capsular types among strains, 472-phosphotransferase system, 383-purified enterotoxins of, 18Staphylococcus epidermidis-accumulation of porphyrins and hemins by, 37Stigmatella aurantiaca (Myxobacterales)-fine structure of fruiting bodies, 856Streptococcal bacteriocins-inactivation of in the intestinal canal and oral

cavity, 888Streptococci-group A, 737-group N, phosphoenolpyruvate in lactose utiliza-

tion by, 603Streptococcus-phospho-,3-glucosidases and ,3-glucosidase perme-

ase in, 434Streptococcus boris-mucopeptide from, 175Streptotoccus dysgalactiae-protective antibody, 527Streptococcus faecalis-survival in the blow fly, 629Streptolydigin-effect on RNA polymurase, 151Streptolydigin-mode of action, 151Streptomyces-phosphorylation of streptomycin by strains of, 401Streptomyces violaceoruber-genetic homologies among strains of, 702Streptomycetes-genetic homologies among, 702Streptomycin-enzymatic phosphorylation of, 401-inhibition of ribosome function, 205Sulfite-formation of thiosulfate from, 210

Taxonomy-by zone electrophoresis, 180Temperature-effect on airborne M. pnteumontiae, 491Temperature-sensitive-DNA synthesis mutant of E. coli, 842-mutant of S. cerevisiae defective in RNA produc-

tion, 807-mutants of D. discoideum, 65-slime molds, 65Terrestrial bacteria-fatty acids in, 366

VOL. 99, 1969 IX

SUBJECT INDEX

Thermophile-intracellular protein breakdown in, 418Thermophilic bacillus-isolation, properties, and kinetics of growth, 414Thiosulfate-formation by D. vulgaris, 210-from sulfite, 210Thymidine-incorporation in mutant bacteria, 681Thymineless death--effect of chloramphenicol, 558-in E. coli, 558Transcriptional mapping-of ribosomal and tRNA cistrons in B. subtilisgenome, 535

Transduction-of merodiploidy induced duplication of recipient

genes, 274Transfer ribonucleic acid-organization of in B. subtilis genome, 535Transformants-kinetics of appearance as a function of time of ex-

posure to DNA, 263Transformation system-of B. licheniformis, 70Transforming markers-sequential entry, 263Trehalase activity--effect of ATP, 904Treponema pallidum-antigenic structure of, 132-Nichols strain, 132Triangularity of cells-effect of different nitrogen sources, 891Tricarboxylic acid cycle-in B. emersonil, 197Trichoderma-complementation in nonconidiating mutants of,

8025', 5', 5'-Trifluoroleucine-effect on protein and RNA synthesis in S. typhi-murium, 107

-effect on repression in S. typhimurium, 101Trigonopsis variabilis-effect of different nitrogen sources on cellular form

of, 891Tryptophan operon-in E. coli, 279-temperature-sensitive repression, 279Tryptophan synthetase mutants-genetic and biochemical studies, 590-of S. cervisiae, 590Tumors-complementation of growth among strains of Agro-

bacterium, 496

Typhoid vaccine-effect on mouse spleen cells, 356-response of mouse strains, 8

Ubiquinone-biosynthesis in E. coli, 450Ultrastructure-of Nocardia cell growth, 876-of putrefactive anaerobe 3679h during sporulation,

824Ultraviolet damage-in S. typhimurium, 350-reversal of, 350Ultraviolet irradiation-effects on E. coli B, 815

Venereal disease-lymphogranuloma venereum, 636Vi antigen-effect on mouse spleen cells, 356-genetic transfer from S. typhosa to E. coli, 358-rabbit immunity to, 13-response of mouse strains, 8Vibrio cholerae-in germ-free mice, 688-somatic 0 antigen relationship to Brucella, 645Vibrio marinus-morphology of, 326-round bodies of, 326Vibrio serotypes-in germ-free mice infected with V. cholerae, 688Vibrios-detection of a lethal factor, 339Vitamin B12-requirement for in mutants of P. denitrificans, 347Vitamins-aromatic, biosynthesis of, 707

Wasting syndrome-estradiol-induced in mice, 902

Yeast cells-conversion to true protoplasts by snail digestive

juice, 885Yeast mutant-defective in RNA production, 807Yeast protoplast-cell wall residues in, 885Yeast trehalase-ATP inhibition of, 904

Zone electrophoresis-of enzymes in bacterial taxonomy, 180

x J. BACTERIOL

JOURNAL

OF

BACTERIOLOGY

VOLUME 99

BALTIMORE, MD. 21202

1969

JOURNAL OF BACTERIOLOGYVOLUME 99 * NUMBER 3 * SEPTEMBER 1969

EDITORIAL BOARDL. LEON CAMPBELL, Editor-in-Chief

University of Illinois, UrbanaL. S. BARON, Editor (1970)

Waler Reed Army Institute ofResearchWashington, D.C.

R. D. DeMOSS, Editor (1970)University ofIllinois, Urbana

ALLEN G. MARR, Editor (1973)University oJ California, DavisERWIN NETER, Editor (1972)

State University ofNew York at Buffalo andChildren's Hospital, Buffalo

Howard I. Adler (1970)R. L. Anderson (1969)Robert Austrian (1969)W. Iae Barksdale (1969)S. S. Barkulis (1969)Alan W. B eirmr (1969)Robert W. Bernlohr (1970)S. Gaylen Bradley (1969)Arnod F. Brodie (1970)Afla Campbell (1969)George B. Chapman (1969)V. P. Crilo (1970)Wallace A. Clyde, Jr. (1970)V. W. Cochrane (1969)S. F. Conti (1969)C. D. Cox (1969)Wlliam J. Cromartie (1969)Roy Curtissm (1969)A. L. Denain (1969)John A. DeMoss (1969)Michael Doudoroff (1969)Martin Dworkin (1970)Ell Englesberg (1969)Frnk B. Engley, Jr. (1969)Stanley Falkow (1969)Wilm W. Ferguson (1969)Leonard Frank (1970)Martin Freundlich (1970)Herman Friedman (1969)Lorrain Friedman (1971)Ruth E. Gordon (1969)Joseph S. Gots (1969)

F. M. Harold (1970)Leonard Hayflick (1969)George Hegeman (1971)James G. HBrsch (1969)D. S. Hoare (1971)John G. Holt (1969)Stanley C. Holt (1971)Paul Howard-Flnders (1969)Milton Huppert (1970)Jerard Hurwitz (1969)John Ingrham (1970)Arthr G. Johnson (1969)R. E. KIallo (1969)Carl Lnanna (1970)Maurice Landy (1970)John H. Law (1971)Edward R. Leadbetter (1970)H. S. Levinon (1969)H. C. Lih n(1970)W. R. L art (1969)Donald G. Lundgren (1971)Elizabeth McCoy (1969)George B. Mackane. (1969)Stanley Marcus (1970)Julius Maormr (1971)Robert G. Martin (1971)M; L. Morse (1969)Frederick C. Neidhardt (1969)M. Nomura (1971)Richard P. Novick (1970)Evelyn L. Ogky (1969)Kennth Paigen (1969)

Robert C. Parlett (1969)Harry D. Peck (1970)H. J. Phaff (1969)Jack Preiss (1971)Roy Repaske (1969)H. V. Rickenberg (1970)C. F. Robinow (1969)W. R. Romig (1969)M; R. J. Salton (1969)Moselio Schaecter (1970)Russell W. s er (1969)David Schlessinger (1971)W. R. Sistrom (1969)Paul F. Smith (1970)Roger Y. Stanier (1970)M. P. Starr (1970)B. A. D. Stocker (1969)Bernard S. Strauss (1969)Paul S. Sypherd (1971)A. L. Taylor (1971)Curtis B. Thoxne (1969)H. E. Umbarger (1970)Lewis W. Wannamaker (1969)Arthu Weissbach (1971)N. E. Welker (1970)R. W. Wheat (1969)Helen R. Whiteley (1969)J. B. Wilson (1969)Meyer J. Wolin (1970)Wilis A. Wood (1969)Barbara E. Wright (1971)Frank E. Young (1970)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIOE. M. FOSTER, President (1969-1970)

DONALD E. SHAY, SecretaryThe Journal of Bacteriology, a publication of the American

Society for Microbiology, 4715 Cordell Ave., Bethesda, Md.20014, is devoted to the advancement and dissemination of funda-mental knowledge concerning bacteria and other microorganisms.The Journal is published monthly, and the twelve numbers aredivided into four volumes per year. The subscription price is$40.00 (Postal Union Countries, $42; other foreign, $43) peryear, including Bacteriological Reviews. Single copies are $4.00(foreign, $4.25). Members of the American Society for Micro-biology may receive the Journal as part of their dues. Correspond-ence relating to reprints, defective copies, availability of backissues, lost or late proofs, disposition of submitted manuscripts,and general editorial matters should be directed to the ASMPublications Office, 4715 Cordell Ave., Bethesda, Md. 20014.

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Correspondence from ASM members relating to membershipdues, member subscriptions, changes of address, incorrect address,incorrect journals, etc., should be directed to the American So-ciety for Microbiology, 1913 I St., N.W., Washington, D.C.20006. Nonmembers of the Society should write to The Williams& Wilkins Co., 428 E. Preston St., Baltimore, Md. 21202, regard-ing institution of nonmember subscriptions and nonreceipt ofjournals. Published monthly by the ASM at 428 E. Preston St.,Baltimore, Md. 21202.

Second class postage paid at Baltimore, Md. 21202, and at addi-tional mailing offices.Made in the United States of America.Copyright 0 1969, American Society for Microbiology.All Rights Reserved

Volume 99 Contents for July Number 1Taxonomy, Ecology, and Morphology and Structure

Vital Staining of Mycoplasma and L-Forms with Cholorazol Black E. MARTHA D.BERLINER, RUTH B. KUNDSIN, AND ELIZABETH N. ALLRED..................... 1

Zone Electrophoresis of Enzymes in Bacterial Taxonomy. JAMES N. BAPTIST, CHARLESR. SHAW, AND MANLEY MANDEL........................................... 180

Pectin-fermenting Bacteria Isolated from the Bovine Rumen. B. A. DEHORITY . ....... 189Bacteria-shaped Gymnoplasts (Protoplasts) of Bacillus subtilis. W. VAN ITERSON AND

J. A. F. OP DEN KAMP.................................................... 304Electron Microscopic Study of a Slime Layer. H. C. JONES, I. L. ROTH, AND W. M.

SANDERS, III ............................................................ 316Morphology and Round Body Formation in Vibrio marinus. R. A. FELTER, R. R.

COLWELL, AND G. B. CHAPMAN............................................ 326Deoxyribonucleic Acid Base Compositions of Selected Mycoplasmas and L-Phase

Variants. CAROL 0. WILLIAMS, RUTH G. WITTLER, AND CARROLL BURRIS....... 341

Infection and ImmunityImmunological Response of Three Mouse Strains to Typhoid Vaccine and Vi Antigen.

VITO M. ESPOSITO, JOHN C. FEELEY, WAYNE D. LEEDER, AND MARGARET PITTMAN... 8Immunological Response of the Rabbit to Vi Antigens. VITO M. ESPOSITO, JOHN C.

FEELEY, AND WAYNE D. LEEDER............................................ 13Ammonium Sulfate Coprecipitation Antibody Determination with Purified Staphylo-

coccal Enterotoxins. JACK GRUBER AND GEORGE C. WRIGHT.................. 18Factors Affecting Immunogenic Activity of Mycobacterial Ribosomal and Ribonucleic

Acid Preparations. ANNE S. YOUMANS AND GUY P. YOUMANS.................. 42Occult Endotoxin in Bacterial Protoplasm. JON A. RUDBACH, KELSEY C. MILNER,

AND EDGAR RIBI......................................................... 51Histamine-sensitizing Factor, Mouse-protective Antigens, and Other Antigens of Some

Members of the Genus Bordetella. R. Ross, J. MUNOZ, AND C. CAMERON......... 57Antigenic Structure of Treponema pallidum, Nichols Strain. II. Extraction of a Poly-

saccharide Antigen with "Strain-specific" Serological Activity. JAMES N. MILLER,J. H. BEKKER, J. H. DEBRUIJN, AND P. C. ONVLEE............................ 132

Interaction of Bdellovibrio bacteriovorus and Host Bacteria. II. Intracellular Growthand Development of Bdellovibrio bacteriovorus in Liquid Cultures. MAZAL VARONAND M. SHILO........................................... ............... 136

New Method for the Detection of a Lethal Factor in Vibrios. KENJI TAKEYA ANDSHOICHI SHIMODORI ............................. 339

Agar Plaque Formation by Mouse Spleen Cells in Response to Vaccination with ViAntigen and Typhoid Vaccines. V. M. ESPOSITO............................. 356

Genetics and Molecular BiologyInheritance of Mating Factors in Nocardial Recombinants. GEORGE H. BROWNELL

AND KAROL L. KELLY .................................................... 25Temperature-sensitive Mutants of Dictyostelium discoideum. WILLIAM F. LOOMIS, JR.. . 65Conditions for Competence in the Bacillus licheniformis Transformation System.

JOHN SPIZIZEN AND LOUISE PRESTIDGE ...................................... 70New Class of Conditional Colicin-tolerant Mutants. ROSA NAGEL DE ZWAIG AND

S. E. LURIA............................................................. 78Locus-specific Changes in Cell Wall Composition Characteristic of Osmotic Mutants

of Neurospora crassa. LAURA R. LIVINGSTON............................... 85Fidelity of Initiation of Protein Synthesis After Premature Chain Termination in

Polarity Mutants. S. J. PARSONS AND R. 0. BURNS.......................... 91Control of Isoleucine, Valine, and Leucine Biosynthesis. VI. Effect of 5', 5', 5'-Trifluoro-

leucine on Repression in Salmonella typhimurium. MARTIN FREUNDLICH ANDJOHN M. TRELA.................................................... ......101

Uncoupling of Protein and Ribonucleic Acid Synthesis by 5', 5', 5'-Trifluoroleucine inSalmonella typhimurium. John M. Trela and Martin Freundlich............... 107

Permeability Properties of Aspergillus nidulans Protoplasts. M. VICTORIA ELORZA,HERBERT N. ARST, JR., DAVID J. COVE, AND CLAUDIO SCAZZOCCHIO............ 113

xiii

Synthesis of Bacterial Flagella.II. PBS1 Transduction of Flagella-specific Markers inBacillus sublilis. GEOFFREY F. GRANT AND MELVIN I. SIMON.................. 116

Binding of Rabbit Gamma Globulin by Competent Bacillus subtilis Cultures. ROBERTJ. ERICKSON, FRANK E. YOUNG, AND WERNER BRAUN.. ...................... 125

Streptomycin Action: Greater Inhibition of Escherichia coli Ribosome Function withExogenous than with Endogenous Messenger Ribonucleic Acid. LucioLUZZATTO,DAVID APIRION, AND DAVID SCHLESSINGER................................... 206

Escherichia coli K-12 Mutants Resistant to Nalidixic Acid: Genetic Mapping andDominance Studies. MICHAEL W. HANE AND THOMAS H. WOOD.............. 238

Chromosomal Location of Ribosomal Protein Cistrons Determined by IntergenericBacterial Mating. DENNIS M. O'NEIL, L. S. BARON, AND PAUL S. SYPHERD...... 242

Characterization of the Deoxyribonucleic Acid of Various Strains of Halophilic Bac-teria. RICHARD L. MOORE AND BRIAN J. MCCARTHY......................... 248

Base Sequence Homology and Renaturation Studies of the Deoxyribonucleic Acidof Extremely Halophilic Bacteria. RICHARD L. MOORE AND BRIAN J. MCCARTHY 255

Sequential Entry of Transforming Markers into Neisseria meningitidis After Chromo-some Alignment. KAARE JYSSUM........................................... 263

Expression of the Arginine Regulon of Escherichia coli W: Evidence for a Second Regu-latory Gene. ELIZABETH C. THEIL, GEORGE W. FORSYTH, AND EVAN E. JONES.... 269

Transduction of Merodiploidy: Induced Duplication of Recipient Genes. C. W. HILL,DINAH SCHIFFER, AND PAUL BERG.......................................... 274

Temperature-sensitive Repression of the Tryptophan Operon in Escherichia coli.KOREAKI ITO, SOTA HIRAGA, AND TAKASHI YURA............................ 279

Electrophoretic Variants of Phosphoglucomutase in Saccharomyces Species. H. C.DOUGLAS, D. PAUL GRINDALL, AND HELEN TALBOTT......................... 287

Protein Specificity and Sexual Morphogenesis in Schizophyllum commune. CHIU-SHENG WANG AND JOHN R. RAPER......................................... 291

Infection of Bacillus stearothermophilus with Bacteriophage Deoxyribonucleic Acid.ULDIS N. STREIPS AND N. E. WELKER .......................................344

Clustering of Functionally Related Genes in Pseudomonas aeruginosa. S. L. ROSENBERGAND G. D. HEGEMAN..................................................... 353

Genetic Transfer of the Vi Antigen from Salmonella typhosa to Escherichia coli. E. M.JOHNSON AND L. S. BARON................................................ 358

Microbial Physiology and MetabolismEffect of Oxygen on Heme and Porphyrin Accumulation from a-Aminolevulinic Acid by

Suspensions of Anaerobically Grown Staphylococcus epidermidis. N. J. JACOBS,J. M. JACOBS, AND G. S. SHENG............................................ 37

Phospholipid Metabolism in Ferrobacillus ferrooxidans. STEVEN A. SHORT, DAVID C.WHITE, AND M. I. H. ALEEM............................................... 142

Mode of Action of Streptolydigin. CHALONGKWAN SIDDHIKOL, JOHN W. ERBSTOESZER,AND BERNARD WEISBLUM.................................................. 151

Estimations of Bacterial Growth Rates in Natural Waters. HOLGER W. JANNASCH... . 156Purification and Reversible Inactivation of the Isocitrate Dehydrogenase from an

Obligate Halophile. JERRY S. HUBBARD AND ALAN B. MILLER................ 161Biochemical Characterization of Lysine Auxotrophs of Staphylococcus aureus. ISABEL

J. BARNES, AMEDEO BONDI, AND ALBERT G. MOAT............................ 169Chemical Studies on the Structure of Mucopeptide Isolated from Streptococcus bovis.

JUDITH KANE, HENRY LACKLAND, W. W. KARAKAWA, AND R. M. KRAUSE....... 175Tricarboxylic Acid Cycle Enzymes and Morphogenesis in Blastocladiella emersonii.

BOEN TIE KHOUW AND HOWARD D. MCCURDY.............................. 197Formation of Thiosulfate from Sulfite by Desulfovibrio vulgaris. BYUNGSE SUH AND

J. M. AKAGI ............................................................ 210

Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Iso-late. J. R. VESTAL AND JEROME J. PERRY .................................. 216

Organization of Enzymes in the Polyaromatic Synthetic Pathway: Separability in Bac-teria. MARY B. BERLYN AND NORMAN H. GILES............................ 222

XiV CONTENTS

CONTENTS ~~~~~~~~xvOrganization of Enzymes in the Common Aromatic Synthetic Pathway: Evidence for

Aggregation in Fungi. S. I. AHMED AND NORMAN H. GILES.................. 231Phospholipid Composition of Bacillus subtilis. J. A. F. OP DEN KAMP, I. REDAI, AND

L. L. M. VAN DEENEN.................................................... 298Amino Acid Antagonist Death in Escherichia coli. M. RABINOVITZ, A. FINKLEMAN,

R. L. REAGAN, AND T. R. BREITMAN........................................ 336Alternate Requirement for Vitamin B12 or Methionine in Mutants of Pseudomonas

denitrificans, a Vitamin B12-producing Bacterium. BARBARA D. LAGO ANDARNOLD L. DEMAIN...................................................... 347

Thermal Reversal of Ultraviolet-Irradiation Damage in Salmonella typhimurium.MICHAEL GOUGH................................................ 350

Volume 99 Contents for August Number 2Taxonomy, Ecology, and Morphology and Structure

Taxonomic Investigations on Expressed and Cryptic Phospho-3-Glucosidases in Entero-bacteriaceae. S. SCHAEFLER AND A. MALAMY......................... ...... 422

Phospho-f-Glucosidases and j3-Glucoside Permeases in Streptococcus, Bacillus, andStaphylococcus. S. SCHAEFLER, A. MALAMY, AND I. GREEN.. 434

Rhodopseudomonas acidophila sp. n., a New Species of the Budding Purple NonsulfurBacteria. NORBERT PFENNIG .............................................. 597

Ultrastructure of Brucella abortus L-Forms Induced by Penicillin in a Liquid and in aSemisolid Medium. BETTY A. HATTEN, MARY L. SCHULZE, SHYI Yi HUANG,AND S. EDWARD SULKIN .................................................. 611

Rhodospirillum tenue sp. n., a New Species of the Purple Nonsulfur Bacteria. NORBERTPFENNIG . .. ... .. .... ... ..... ... ... ....... .. .. .. .. ..... .... 619

Infection and ImmunityAntifungal Effects of Peroxidase Systems. R. I. LEHRER.......................... 361Inhibition of Protease Production of Various Bacteria by Ammonium Salts: Its Ef-

fect on Toxin Production and Virulence. PINGHUI V. LiU AND HSI-CHIA HSIEH .... 406Correlation Between Growth Inhibition and the Binding of Various Penicillins and

Cephalosporins to Staphylococcus aureus. JOHN R. EDWARDS AND JAMES T.PARK.................................................................. 459

Isolation of an Antigen of Neisseria gonorrhoeae Involved in the Human ImmuneResponse to Gonococcal Infection. JOHN D. SCHMALE, DAN G. DANIELSSON,JOSEPH F. SMITH, LINDA LEE, AND WILLIAM L. PEACOCK, JR...................... 469

Evidence for a Multiplicity of Capsular Types Among Staphylococcus aureus Strains.NICK H. MAVERAKIS AND BILL B. WILEY................................... 472

R Factor Transmission In Vivo. H. JAROLMEN AND G. KEMP..... .................. 487Tumor Growth Complementation Among Strains of Agrobacterium. JAMES A. LIPPIN-

COTT AND BARBARA B. LIPPINCOTT ......................................... 496Influence of 0 Side-Chains on the Attachment of the Felix 0-1 Bacteriophage to Sal-

monella Bacteria. A. A. LINDBERG AND T. HOLME......... 513Effect of Bacterial Contamination on Cecal Size and Cecal Contents of Gnotobiotic

Rodents. WALTER J. LOESCHE............................................. 520Estimation and Identification of Streptococcus dysgalactiae Protective Antibody in

Sera of Rabbits and Cattle. D. M. STARK, N. L. NORCROSS, AND J. E. LEAHEY..... 527Induced Bactericidal Response in the Hagfish. RONALD T. ACTON, PETER F. WEIN-

HEIMER, W. H. HILDEMANN, AND E. EDWARD EVANS.......................... 626

Genetics and Molecular BiologyAmino Acid Differences in a 30S Ribosomal Protein from Two Strains of Escherichia

coli. PAUL S. SYPHERD .................... .............................. 379Genetic Separation of the Inosinic Acid Cyclohydrolase-Transformylase Complex of

CONTENTS xv

Salmonella typhimurium. JOSEPH S. GOTS, FRAM R. DALAL, AND SUSAN R.SHUMAS .. .... .......... .. ........ ...................................... 441

Biosynthesis of Ubiquinone in Escherichia ccli K-i12: Location of Genes Affecting th44Metabolism of 3-Octaprenyl-4-hydroxybenzoic Acid and 2-Octaprenylphenol.G. B. Cox, I. G. YOUNG, L. M. MCCANN, AND F. GIBSON.450

Transcriptional Mapping: Functional Organization of the Ribosomal and TransferRibonucleic Acid Cistrons in the Bacillus subtilis Genome. MICHAEL BLEYMAN,MASATOSHI KONDO, NORMAN HECHT, AND CARL WOESE.535

Thymineless Death in Escherichia coli: Inactivation and Recovery. DONALD J. CUM-MINGS AND ALVIN R. Kusy.558

Reversion Instability in the Galactose Operon of Escherichia coli. M. L. MORSEAND BEATRICE F. POLLOCK.567

Microbial Physiology and MetabolismFatty Acids in the Lipids of Marine and Terrestrial Nitrifying Bacteria. M. BLUMER,

T. CHASE, AND S. W. WATSON.366Fatty Acid Composition of Candida utilis as Affected by Growth Temperature and

Dissolved-Oxygen Tension. C. M. BROWN AND A. H. ROSE.371Phosphotransferase System of Staphylococcus aureus: Its Requirement for the Ac-

cumulation and Metabolism of Galactosides. WOLFGANG HENGSTENBERG, W.K. PENBERTHY, KATHERINE L. HILL, AND M. L. MORSE.383

Role of Sodium in Determining Alternate Pathways of Aerobic Citrate Catabolism inAerobacter aerogenes. R. W. O'BRIEN AND JOSEPH R. STERN.389

Enzymatic Analysis of the Requirement for Sodium in Aerobic Growth of Salmonellatyphimurium on Citrate. R. W. O'BRIEN, G. M. FROST, AND JOSEPH R. STERN. 395

Enzymatic Phosphorylation of Streptomycin by Extracts of Streptomycin-producingStrains of Streptomyces. ANN L. MILLER AND JAMES B. WALKER.401Prototrophic Thermophilic Bacillus: Isolation, Properties, and Kinetics of Growth.ISRAEL EPSTEIN AND NATHAN GROSSOWICZ.414

Intracellular Protein Breakdown in a Thermophile. ISRAEL EPSTEIN AND NATHANGROSSOWICZ.418

Role of Ammonium Ion in the Biosynthesis of ,B-Nitropropionic Acid. PAUL D. SHAWAND ANTHONY B. DEANGELO 463

Biosynthesis of Glucosyl Diglycerides by Mycoplasma laidlawii Strain B. PAUL F.SMITH.480

Effect of Temperature on Survival of Airborne Mycoplasma pneumoniae. D. N.WRIGHT, G. D. BAILEY, AND L. J. GOLDBERG.491

Effect of Bacteria on Chemotaxis in the Cellular Slime Molds. THEO M. KONIJN. 503Identification of Adenosine-3', 5-Monophosphate as the Bacterial Attractant for

Myxamoebae of Dictyostelium discoideum. T. M. KONIJN, J. G. C. VAN DEMEENE, Y. Y. CHANG, D. S. BARKLEY, AND J. T. BONNER.510

Growth of Physarum flavicomum and Physarum rigidum in Chemically Defined Mini-mal Media. HENRY R. HENNEY, JR., AND THOMAS LYNCH.531

Dissimilation of Methionine by Fungi. JosE RUIZ-HERRERA AND ROBERT L. STARKEY. . 544Kinetic Studies of Iron Oxidation by Whole Cells of Ferrobacillusferrooxidans. C. A.

SCHNAITMAN, M. S. KORCZYNSKI, AND D. G. LUNDGREN...................... 552Respiration in Blue-Green Algae. JOHN BIGGINS................................ 570Gelatin-induced Reversion of Protoplasts of Bacillus subtilis to the Bacillary Form:

Biosynthesis of Macromolecules and Wall During Successive Steps. OTTO E.LANDMAN AND ARNOLD FORMAN........................................... 576

Genetic and Biochemical Studies of Partially Active Tryptophan Synthetase Mutantsof Saccharomyces cerevisiae. THOMAS R. MANNEY, WOLFGANG DUNTZE, NANCYJANOSKO, AND JORGE SALAZAR............................................ 590

Involvement of Phosphoenolpyruvate in Lactose Utilization of Group N Streptococci.L. L. MCKAY, L. A. WALTER, W. E. SANDINE, AND P. R. ELLIKER................ 603

Polyuridylic Acid-directed Phenylalanine Incorporation in Minicell Extracts. J. A.FRALICK, W. D. FISHER, AND H. I. ADLER................................... 621

xvi CONT'ENTS

CONTENTS xvii

Oxidation of Isomaltose by Pseudomonas taetrolens. M. STERNBERG AND L. B. LOCK-WOOD............................................................... 623

Feedback Inhibition of Lysine Biosynthesis in Yeast. ANTHONY F.Tucci............ 624

Volume 99 Contents for September Number 3Taxonomy, Ecology, and Morphology and Structure

Deoxyribonucleic Acid Homology and Relative Genome Size in Mycoplasma. ANNEM. PETERSON AND MARY E. POLLOCK ........................................ 639

Genetic Homologies Among Streptorrnyces violaceoruber Strains. A. M. MONSON,S. G. BRADLEY, L. W. ENQUIST, AND GRISELDA CRUCES........................ 702

Ultrastructure of Putrefactive Anaerobe 3679h During Sporulation. LEATRICE MIURASANTO, HANS R. HOHL, AND HILMER A. FRANK.............................. 824

Fine Structure of Fruiting Bodies of Stigmatella aurantiaca (Myxobacterales). HERBERTVOELZ AND HANS REICHENBACH............................................ 856

Ultrastructure of Nocardia Cell Growth and Development on Defined and ComplexAgar Media. B. L. BEAMAN AND D. M. SHANKEL........................... 876

Cell Wall Residues in Yeast Protoplast Preparations. J. S. D. BACON, D. JONES, ANDP. OTTOLENGHI .......................................................... 885

Infection and Immunity

Salmonella Suppression by Known Populations of Bacteria in Flies. BERNARDGREENBERG ............................................................. 629

Lymphogranuloma venereum. II. Characterization of Some Recently Isolated Strains.JULIUS SCHACHTER AND K. F. MEYER ...................................... 636

Somatic 0 Antigen Relationship of Brucella and Vibrio cholerae. JOHN C. FEELEY... 645Properties and Characteristics of Bacteriocin from Serratia marcescens. JOHN D.

FOULDS AND DAVID SHEMIN................................................ 655Concomitant Synthesis of Bacteriocin and Bacteriocin Inactivator from Serratia

marcescens. JOHN D. FOULDS AND DAVID SHEMIN ........................... 661Progressive Changes of Vibrio Serotypes in Germ-free Mice Infected with Vibrio

cholerae. R. BRADLEY SACK AND CARL E. MILLER.......................... 688Comparison of the Pathogenicity for Mice of Mycobacterium fortuitum and Myco-

bacterium abscessus. HAJIME SAITO AND HIROMICHI TASAKA ..... ............. 851Morphological Changes of Mycobacterium lepraemurium Grown in Cultures of Mouse

Peritoneal Macrophages. Y. T. CHANG AND R. N. ANDERSEN ..... ............. 867Inactivation of Bacteriocins in the Intestinal Canal and Oral Cavity. J. KELSTRUP

AND R. J. GIBBONS ...................................................... 888Adaptation of Meningococcal L-Forms to Agar-free Liquid Medium. RONALD H.

STEWART AND D. N. WRIGHT ........................................... 899Estradiol-induced Wasting Syndrome in Conventionally Reared and Germ-free Mice.

GEORGE G. HATCH AND NORMAN D. REED ................................. 902Plaque Assay System for Several Species of Rickettsia. JOSEPH E. MCDADE, JACK R.

STAKEBAKE, AND PETER J. GERONE ........................................ 910

Genetics and Molecular Biology

Escherichia coli ras Locus: Its Involvement in Radiation Repair. JAMES R. WALKER.. 713Regulation of Chromosome Replication in Bacillus subtilis: Effect of Amino Acid Star-

vation in Strain 168. JAMES C. COPELAND .................................. 730Origin and Sequence of Chromosome Replication in Neisseria meningitidis: Influ-

ence of a Genetic Factor Determining Competence. KAARE JYSSUM ............ 757Peptide Synthesis by Extracts from Bacillus subtilis Spores. HELEN L. BISHOP, LLOYD

K. MIGITA, AND ROY H. DoI.............................................. 771"Self-feeding" Strain of Salmonella typhimurium with a Mutation in the trpB Gene

xviii CONTENTS

and Nutritional Requirements of trpA Gene Mutants. COLIN STUTTARD ANDG. W. P. DAWSON ...................................................... 779

Leakiness of Pleiotropic Maltose-negative, Bacteriophage X-resistant Mutants ofEscherichia coli K-12. A. RONEN AND A. ZEHAVI ............................ 784

Complementation in Nonconidiating Mutants of Trichoderma. DVORA WEINMAN-GREENSHPAN AND ESRA GALUN............................................ 802

Temperature-sensitive Yeast Mutant Defective in Ribonucleic Acid Production. H.TERRY HUTCHISON, LELAND H. HARTWELL, AND CALVIN S. MCLAUGHLIN ........ 807

Unlinking of Cell Division from Deoxyribonucleic Acid Replication in a Tempera-ture-sensitive Deoxyribonucleic Acid Synthesis Mutant of Escherichia coli.MASAYORI INOUYE......................................................... 842

Mode of Nicotinamide Adenine Dinucleotide Utilization by Escherichia coli. R. K.GHOLSON, GERALD J. TRITZ, THOMAS S. MATNEY, AND A. J. ANDREOLI .... ........ 895

Mode of Action of Colicin A. ROSA NAGEL DE ZWAIG ........................... 913

Microbial Physiology and MetabolismHigh Molecular Weight Phosphorus Compound in Nucleic Acid Extracts of the Slime

Mold Physarumpolycephalum. H. W. SAUER, K. L. BABCOCK, AND H. P. RUSCH. 650Hexuronic Acid Dehydrogenase of Agrobacterium tumefaciens. YUNG FENG CHANG

AND DAVID SIDNEY FEINGOLD.............................................667Differential Lytic Response of Enterococci Associated with Addition Order of Lysozyme

and Anions. ROBERT H. METCALF AND R. H. DEIBEL ........................ 674Specificity and Efficiency of Thymidine Incorporation in Escherichia coli Lacking

Thymidine Phosphorylase. WALTON L. FANGMAN .......................... 681Biosynthesis of Desthiobiotin in Cell-free Extracts of Escherichia coli. C. H. PAI... . 696Regulation of 3-Deoxy-D-Arabino-Heptulosonic 7-phosphate Acid Synthetase Activity

in Relation to the Synthesis of the Aromatic Vitamins in Escherichia coli K-12. B.J. WALLACE AND J. PITTARD .............................................. 707

Nitrate Reductase Complex of Escherichia coli K-12: Participation of Specific FormateDehydrogenase and Cytochrome bi Components in Nitrate Reduction. JOSERUIZ-HERRERA AND J. A. DEMOSS .................................. 720

Effect of Culture Medium Composition and pH on the Production of M Protein andProteinase by Group A Streptococci. JAY 0. COHEN ........................ 737

Growth and Sporulation of Bacillus subtilis Mutants Blocked in the Pyruvate Dehydro-genase Complex. ERNST FREESE AND UTA FORTNAGEL ........................ 745

Dissimilation of Methionine by a Demethiolase of Aspergillus Species. JOSE RuIz-HERRERA AND ROBERT L. STARKEY......................................... 764

Regulation. of the Biosynthesis of Amino Acids of the Aspartate Family in ColiformBacteria and Pseudomonads. G. N. COHEN, R. Y. STANIER, AND GISELE LEBRAS.................................................................. 791

Effects of Ultraviolet Radiation on Respiration and Growth in Radiation-resistant andRadiation-sensitive Strains of Escherichia coli B. BARBARA A. HAMKALO ANDP. A. SWENSON .......................................................... 815

Cation-activated Nucleotidase in Cell Envelopes of a Marine Bacterium. J. THOMPSON,M. L. GREEN, AND F. C. HAPPOLD ......................................... 834

Effect of Different Nitrogen Sources on the Cellular Form of Trigonopsis variabilis.VACLAV AASEK AND GEORGE E. BECKER ..................................... 891

Serine Biosynthesis in Desulfovibrio desulfuricans. GENO J. GERMANO AND KENNETHE. ANDERSON ............................................................ 893

Oxidation of Ammonia by Spheroplasts of Nitrosomonas europaea. ISAMU SUZUKI ANDS. C. KWOK ................................... 897

Adenosine Triphosphate Inhibition of Yeast Trehalase. ANITA D. PANEK.......... 904Evidence for a Reductive Pathway for the Anaerobic Metabolism of Benzoate. MARK

GUYER AND GEORGE HEGEMAN.................................... 906Growth of Parasitic Mycoplasma Without Serum or Serum Fraction. HENRYKA KUR-

ZEPA, LINDA FLINTON, AND P. J. VANDEMARK............................... 908