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Crystal H. Shelton Crystal H. Shelton

Research AssociateResearch Associate

Scientific AffairsScientific Affairs

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These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Important Ingredients for Important Ingredients for Neurological, Metabolic and Neurological, Metabolic and

Digestive SupportDigestive Support Folic AcidFolic Acid Vitamin B6 (P5P)Vitamin B6 (P5P) Vitamin B12Vitamin B12 Vitamin B3Vitamin B3 Vitamin CVitamin C

Vitamin AVitamin A ZincZinc CalciumCalcium MagnesiumMagnesium

Folinic AcidFolinic Acid Food sources include:Food sources include:

Leafy green Leafy green vegetables (like vegetables (like spinach and turnip spinach and turnip greens), fruits (like greens), fruits (like citrus fruits and citrus fruits and juices), and dried juices), and dried beans and peas are all beans and peas are all natural sources of natural sources of folate folate

Essential for numerous Essential for numerous processes in the body and processes in the body and supports the normal supports the normal development of the development of the nervous system nervous system

Folinic acid (tetrahydrofolic Folinic acid (tetrahydrofolic acid)is the metabolically acid)is the metabolically active form of folic acid, active form of folic acid, high bioavailability high bioavailability

Folinic AcidFolinic Acid Supports the conversion of sulfur-containing Supports the conversion of sulfur-containing

compounds to glutathione, studies show low compounds to glutathione, studies show low glutathione due to abnormalities in methionine glutathione due to abnormalities in methionine pathway pathway

Antioxidant Antioxidant DetoxificationDetoxification

Because of folic acid’s critical role in neurological Because of folic acid’s critical role in neurological function, it can help to maintain healthy sleep quality, function, it can help to maintain healthy sleep quality, and help facilitate the normal development of and help facilitate the normal development of communication and social interaction skillscommunication and social interaction skills

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Vitamin B6 (P5P or Vitamin B6 (P5P or Pyridoxine-5-Phosphate)Pyridoxine-5-Phosphate)

Food sources include: Food sources include: Beans, meat, poultry, Beans, meat, poultry,

fish, and some fruits fish, and some fruits and vegetablesand vegetables

Master vitamin for Master vitamin for numerous metabolic numerous metabolic pathways in the body pathways in the body

Pyridoxal-5-phosphate is Pyridoxal-5-phosphate is the active form of vitamin the active form of vitamin B6B6

B6 is critical for the B6 is critical for the normal development of normal development of language, attention, language, attention, alertness, etc. alertness, etc.

To be used by the body, To be used by the body, B6 must be converted to B6 must be converted to P5P in the liverP5P in the liver

B6 and OxalatesB6 and Oxalates

Acts as a cofactor for enzymes that Acts as a cofactor for enzymes that degrade oxalatesdegrade oxalates

Molecules present in many plants and fruit, Molecules present in many plants and fruit, all seeds and nuts, grainsall seeds and nuts, grains

When the gut is leaky higher quantities of When the gut is leaky higher quantities of oxalates are absorbed from foods oxalates are absorbed from foods

Vitamin B6 (P5P or Vitamin B6 (P5P or Pyridoxine-5-Phosphate)Pyridoxine-5-Phosphate)

Vitamin B6 is the most studied nutrient Vitamin B6 is the most studied nutrient among children with neuroglogical, among children with neuroglogical, metabolic and digestive issues and is often metabolic and digestive issues and is often given in combination with magnesium. given in combination with magnesium.

B6 naturally requires extra magnesium to B6 naturally requires extra magnesium to be effectivebe effective

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Methylcobalamin Methylcobalamin (Vitamin B12)(Vitamin B12)

Food sources include:Food sources include: Naturally found in foods Naturally found in foods

that come from animals, that come from animals, including fish, meat, including fish, meat, poultry, eggs, milk, and poultry, eggs, milk, and milk products milk products

A water-soluble vitamin A water-soluble vitamin that supports normal that supports normal nerve cell activity, DNA nerve cell activity, DNA replication, and replication, and production of the production of the mood-affecting mood-affecting substance SAMe (S-substance SAMe (S-adenosyl-L-methionine)adenosyl-L-methionine)

Promotes normal Promotes normal conversion of sulfur-conversion of sulfur-containing compounds containing compounds to glutathione to glutathione (antioxidant and (antioxidant and detox)detox)

Vitamin B12 Vitamin B12 deficiency is deficiency is commonly found in commonly found in children with children with neurological, neurological, metabolic and metabolic and digestive needs digestive needs

Methylcobalamin Methylcobalamin (Vitamin B12)(Vitamin B12)

Methylated form of B12, Methylated form of B12, evidence indicates evidence indicates methylcobalamin is methylcobalamin is utilized more efficiently utilized more efficiently than cobalaminthan cobalamin

Methylcobalamin doesn't Methylcobalamin doesn't have to engage the have to engage the body's resources to body's resources to convert it into coenzyme convert it into coenzyme formform

Methylcobalmin is closely Methylcobalmin is closely allied with the folic acid allied with the folic acid biochemical pathway and biochemical pathway and promotes normal promotes normal detoxificationdetoxification

The most highly The most highly reduced form reduced form of of vitamin B12 possiblevitamin B12 possible

Why Methylcobalamin?Why Methylcobalamin? Already metabolically Already metabolically

activeactive Maximally reducedMaximally reduced Less of a strain on our Less of a strain on our

bodies' antioxidant bodies' antioxidant capacitycapacity

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Vitamin B3 Vitamin B3 (Niacin/Niacinamide)(Niacin/Niacinamide)

Food sources include:Food sources include: Beets, brewer's yeast, Beets, brewer's yeast,

beef liver, beef kidney, beef liver, beef kidney, pork, turkey, chicken, pork, turkey, chicken, veal, fish, tuna, peanuts veal, fish, tuna, peanuts

A water-soluble vitamin A water-soluble vitamin necessary for numerous necessary for numerous metabolic pathways in metabolic pathways in the bodythe body

Involved in pathways Involved in pathways that sustain and renew that sustain and renew the body’s tissuesthe body’s tissues

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Vitamin CVitamin C

Water-soluble vitamin, Water-soluble vitamin, also known as ascorbic also known as ascorbic acidacid

Food sources include:Food sources include: Oranges, green peppers, Oranges, green peppers,

strawberries, other fruit, strawberries, other fruit, tomatoes, broccolitomatoes, broccoli

Promotes normal tissue Promotes normal tissue repair, bone healing, repair, bone healing, immune function among immune function among other things other things

Proven antioxidantProven antioxidant

Cofactor for dopamine Cofactor for dopamine production, helps production, helps maintain brain healthmaintain brain health

Vitamin C also promotes Vitamin C also promotes glutathione levels, glutathione levels, another important another important antioxidant in the body antioxidant in the body

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Vitamin CVitamin C A large study by James et al. confirmed low glutathione A large study by James et al. confirmed low glutathione

levels in children and associations with abnormalities in levels in children and associations with abnormalities in their methionine pathway, i.e. increased oxidative stresstheir methionine pathway, i.e. increased oxidative stress

153 children studied for transmethylation and transulfuration 153 children studied for transmethylation and transulfuration issue and 565 children evaluated for common polymorphic issue and 565 children evaluated for common polymorphic variants known to modulate these pathwaysvariants known to modulate these pathways

James et al. Metabolic endophenotype and related genotypes are James et al. Metabolic endophenotype and related genotypes are associated with oxidative stress in children with autism. associated with oxidative stress in children with autism. American Journal of Medical GeneticsAmerican Journal of Medical Genetics. 141B(8):947-56, 2006. . 141B(8):947-56, 2006.

Vitamin C supported red blood cell glutathione levels in Vitamin C supported red blood cell glutathione levels in college studentscollege students

Johnston et al. Vitamin C elevates red blood cell glutathione in healthy Johnston et al. Vitamin C elevates red blood cell glutathione in healthy adults. adults. American Journal of Clinical NutritionAmerican Journal of Clinical Nutrition. 58(1):103-5, 1993. . 58(1):103-5, 1993.

Vitamin A (Beta-Vitamin A (Beta-Carotene)Carotene)

Essential vitamin – Essential vitamin – Beta-Carotene Beta-Carotene → → retinal retinal

form of vitamin A form of vitamin A → → retinolretinol

Food sources include:Food sources include: Yellow and orange fruits Yellow and orange fruits

(carrots, papaya. mangos), (carrots, papaya. mangos), green leafy veggies (spinach)green leafy veggies (spinach)

Supports normal cell Supports normal cell growth and differentiation, growth and differentiation, especially in tissues of the especially in tissues of the gut and brain, helps gut and brain, helps maintain vision and maintain vision and immune functionsimmune functions

Powerful antioxidantPowerful antioxidant

Vitamin A deficiency is Vitamin A deficiency is commonly found in commonly found in children with children with neurological, metabolic neurological, metabolic and digestive needs and digestive needs

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ZincZinc Food sources include:Food sources include:

Oysters, red meat, beans, Oysters, red meat, beans, nuts, certain seafood, nuts, certain seafood, whole grainswhole grains

An essential mineralAn essential mineral

When zinc levels are down, When zinc levels are down, copper levels are often copper levels are often increasedincreased

Children who need Children who need neurological or digestive neurological or digestive support often have an support often have an abnormal zinc to copper abnormal zinc to copper ratioratio

Copper imbalance in Copper imbalance in children is also associated children is also associated with delayed development, with delayed development, anti-social and hyperactive anti-social and hyperactive behavior, learning behavior, learning difficulties difficulties

Because zinc helps Because zinc helps facilitate the excretion of facilitate the excretion of excess copper, it is excess copper, it is important to make sure a important to make sure a multivitamin has an multivitamin has an adequate amount of zinc adequate amount of zinc

Avoid copper-containing Avoid copper-containing supplementssupplements

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CalciumCalcium Food sources include:Food sources include:

Dairy, milk, yogurt, Dairy, milk, yogurt, cheese, green leafy cheese, green leafy vegetablesvegetables

This mineral promotes This mineral promotes normal:normal: bone healthbone health nerve transmission nerve transmission muscle contractionmuscle contraction vasodilationvasodilation enzyme reactionsenzyme reactions respirationrespiration among many othersamong many others

Calcium Calcium supplementation may supplementation may help to promote normal help to promote normal oxalate absorptionoxalate absorption Bind to dietary calcium Bind to dietary calcium

and is removed through and is removed through the digestive tract and the digestive tract and not absorbed not absorbed

It’s especially It’s especially important to important to supplement calcium in supplement calcium in those on a casein-free those on a casein-free diet diet

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MagnesiumMagnesium Food sources include:Food sources include:

Green vegetables Green vegetables (spinach), legumes (beans (spinach), legumes (beans and peas), nuts and seeds and peas), nuts and seeds

Essential mineralEssential mineral

Supports the proper Supports the proper functioning of many functioning of many enzymes, including those enzymes, including those responsible for the responsible for the production of production of neurotransmitters in the neurotransmitters in the brain brain

Combination of B6 and Combination of B6 and magnesiummagnesium

4.6:1 and 11:1 4.6:1 and 11:1 (combination)(combination)

Recent Multivitamin Recent Multivitamin ResearchResearch

A small pilot study found that a strong, balanced multi-vitamin/mineral supplement resulted in improvements in sleep and promoted normal gut function in children, and possibly in other areas

20 children ages 3-8 years old Given a multivitamin/multimineral supplement for 3 months Based on parental assessment, there were statistically

significant improvements in sleep and supported GI comfort Also determined a functional need for more vitamin B6 and

vitamin C

Adams J et al. Pilot study of a moderate dose Adams J et al. Pilot study of a moderate dose multivitamin/mineral supplement for children with autistic multivitamin/mineral supplement for children with autistic spectrum disorder. spectrum disorder. Journal of Alternative and Journal of Alternative and Complementary MedicineComplementary Medicine. 10(6):1033-1039, 2004.. 10(6):1033-1039, 2004.

ConclusionConclusion The need for increased support in the areas of The need for increased support in the areas of

neurology, metabolism and digestion is more prevalent neurology, metabolism and digestion is more prevalent in our society than ever before, but there are proven in our society than ever before, but there are proven nutritional approaches that may help:nutritional approaches that may help:

Recap of important ingredients:Recap of important ingredients: Folinic acidFolinic acid P5P (Vitamin B6)P5P (Vitamin B6) MethylcobalaminMethylcobalamin Vitamin CVitamin C ZincZinc CalciumCalcium MagnesiumMagnesium

Isotonix Might-a-MinsIsotonix Might-a-Mins®® SpectrumSpectrum

MultivitaminMultivitamin

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Isotonix Might-a-MinsIsotonix Might-a-Mins®® SpectrumSpectrum

MultivitaminMultivitamin Helps to promote normal levels of vitamins and Helps to promote normal levels of vitamins and

minerals in individuals who need neurological, minerals in individuals who need neurological, digestive or metabolic supportdigestive or metabolic support

Helps maintain the normal production of Helps maintain the normal production of neurotransmittersneurotransmitters

Promotes/supports a healthy nervous systemPromotes/supports a healthy nervous system

Helps maintain brain healthHelps maintain brain health

Promotes a healthy development of sensory Promotes a healthy development of sensory and communication skills. and communication skills.

Isotonix Might-a-MinsIsotonix Might-a-Mins®® SpectrumSpectrum

MultivitaminMultivitamin Provides strong antioxidant defenseProvides strong antioxidant defense Combats free radicalsCombats free radicals Promotes normal tissue and cell growthPromotes normal tissue and cell growth Provides nutrients essential for numerous Provides nutrients essential for numerous

processes in the bodyprocesses in the body Supports optimal immune functionsSupports optimal immune functions Supports skeletal health and growthSupports skeletal health and growth Supports healthy teeth and gums Supports healthy teeth and gums Supports growth and strength of teeth and bonesSupports growth and strength of teeth and bones Supports cognitive health and development Supports cognitive health and development Supports healthy vision and eyesSupports healthy vision and eyes Supports a healthy heartSupports a healthy heart

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Institute Publication. 40 (April), 2007. Institute Publication. 40 (April), 2007. Adams J et al. Pilot study of a moderate dose multivitamin/mineral Adams J et al. Pilot study of a moderate dose multivitamin/mineral

supplement for children with autistic spectrum disorder. Journal of supplement for children with autistic spectrum disorder. Journal of Alternative and Complementary Medicine. 10(6):1033-1039, 2004.Alternative and Complementary Medicine. 10(6):1033-1039, 2004.

Megson M. Is autism a G-alpha protein defect reversible with natural Megson M. Is autism a G-alpha protein defect reversible with natural vitamin A? Medical Hypotheses. 54(6):979-983, 2000.vitamin A? Medical Hypotheses. 54(6):979-983, 2000.

Lelord G et al. Effects of pyridoxine and magnesium on autistic symptoms: Lelord G et al. Effects of pyridoxine and magnesium on autistic symptoms: initial observations. Journal of Autism Developmental Disorders. 11(2):219-initial observations. Journal of Autism Developmental Disorders. 11(2):219-229, 1981.229, 1981.

Martineau J et al. Effects of vitamin B6 on averaged evoked potential in Martineau J et al. Effects of vitamin B6 on averaged evoked potential in infantile autism. Biological Psychiatry. 16(7):627-641, 1981.infantile autism. Biological Psychiatry. 16(7):627-641, 1981.

Martineau J et al. Vitamin B6, magnesium, and combined B6-Mg: Martineau J et al. Vitamin B6, magnesium, and combined B6-Mg: therapeutic effects in childhood autism. Biological Psychiatry. 20(5):467-therapeutic effects in childhood autism. Biological Psychiatry. 20(5):467-478, 1985.478, 1985.

Lonsdale D et al. Treatment of autism spectrum children with thiamine Lonsdale D et al. Treatment of autism spectrum children with thiamine tetrahydrofurfuryl disulfide: A pilot study. Neuroendocrinology Letters. tetrahydrofurfuryl disulfide: A pilot study. Neuroendocrinology Letters. 23(4):303-308, 2002.23(4):303-308, 2002.

Rimland B et al. The effect of high doses of vitamin B6 on autistic children: Rimland B et al. The effect of high doses of vitamin B6 on autistic children: a double-blind cross-over study. American Journal of Psychiatry. a double-blind cross-over study. American Journal of Psychiatry. 135(4):472, 1978.135(4):472, 1978.

Rimland B. Vitamin B6 (and magnesium) in the treatment of autism. Rimland B. Vitamin B6 (and magnesium) in the treatment of autism. Autism Research Review International. 1(4), 1987.Autism Research Review International. 1(4), 1987.

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autism. Progress in Neuropsycho-pharmacology and Biological Psychiatry. autism. Progress in Neuropsycho-pharmacology and Biological Psychiatry. 17(2):765-774, 1993.17(2):765-774, 1993.

Rimland B. Vitamin C in the Prevention and Treatment of Autism. Autism Research Rimland B. Vitamin C in the Prevention and Treatment of Autism. Autism Research Review International. 12:3, 1998.Review International. 12:3, 1998.

Martineau J et al. Brief report: an open middle-term study of combined vitamin B6-Martineau J et al. Brief report: an open middle-term study of combined vitamin B6-magnesium in a subgroup of autistic children selected on their sensitivity to this magnesium in a subgroup of autistic children selected on their sensitivity to this treatment.  Journal of Autism and Developmental Disorders. 18:435-447, 1988.treatment.  Journal of Autism and Developmental Disorders. 18:435-447, 1988.

Martineau J et al. Electrophysiological effects of fenfluramine or combined vitamin Martineau J et al. Electrophysiological effects of fenfluramine or combined vitamin B6 and magnesium on children with autistic behavior. Developmental Medicine B6 and magnesium on children with autistic behavior. Developmental Medicine and Child Neurology. 31:728-736, 1989. and Child Neurology. 31:728-736, 1989.

Audhya T. (2002, October). Laboratory indices of vitamin and mineral deficiency Audhya T. (2002, October). Laboratory indices of vitamin and mineral deficiency in autism.  Paper presented at the Defeat Autism Now! Conference, San Diego, in autism.  Paper presented at the Defeat Autism Now! Conference, San Diego, California. California.

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