is piezoelectric surgery the new gold-standard in oral ......surgical procedures in dentistry and...

7
| 12 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 13 | ABSTRACT Mills, drills, burs and low-frequency oscillating saws were the only tools for bone-cutting for a long time and their use rarely questioned on their biological and physiological effects on bone. With the emergence of new technologies in bone-cutting - such as lasers and piezoelectric cutting devices - more and more experimental and clinical studies shed light on the differences in the bone healing-cycle when traditional instrumentation is compared to these new technologies. A search in medical and biomedical databases was performed and molecular-biologic, micromorphologic, histologic, experimental and clinical comparative studies were selected, excluding case-reports and unclear methodology. Experimental and clinical findings then were summarized and compared against each other by their micromorphologic, histologic, biologic and clinical effects resulting in a comparative overview with impact to the clinician’s daily routine-work. At the current state of secure knowledge, scientific literature suggests piezoelectric surgical tools for bone-cutting to provide the highest clinical precision, the least procedural bone-loss, an improved bone-healing and best “ease-of-use”. KEYWORDS Piezoelectric surgery, Piezosurgery, Piezotome-surgery, Ultrasonic bone cutting, Bone-cutting instruments, Osteotomy, Drill, Bur, Mill, Laser. Is Piezoelectric Surgery the New Gold-Standard in Oral Surgery and Implantology? A Scientific Literature Review Troedhan Angelo - MD, DMD, PhD Specialist Craniomaxillofacial Surgery, Specialist Dentistry V.Prof. Health Science University Vientiane – Lao PDR | Vienna, Austria | [email protected] Ziad Tarek Mahmoud - DDS, MSc Lecturer of Oral and Maxillofacial Surgery, Faculty of Dentistry, Alexandria University | Alexandria, Egypt | [email protected] INTRODUCTION Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery, bone-management, guided bone regeneration (GBR), dental implant insertion, orthognathic surgery etc.) are performed traditionally with chisels, burs, osteotomes and low- frequency oscillating saws. However, rotating or low-frequency oscillating instruments are very difficult to handle when used on cortical and trabecular bone due to its procedural high physical torque-moment. The result is a loss of tactile sensitivity due to the requirement of pressure on the hand-piece, difficulty in the determination of cutting depth and iatrogenic impairment in undesired areas due to a failure in the accurate adjustment of the speed of a rotating bur or oscillating saw, particularly when ultimate precise osteotomies are essential. 1 When chisels or osteotomes are used, labyrinthine concussion of the inner ear, positional vertigo or even brain concussion may occur. 2,3 Major and unavoidable medical drawbacks of the use of motorized rotary, reciprocal or low-frequency oscillating instruments in bone-cutting are enormous procedural bone losses, significant bone-necrosis due to overheating and the high risk of soft tissue injury to important anatomical structures such as the inferior alveolar nerve or maxillary sinus-membrane and deposition of metal shavings and bacterial contamination. 4-7 Burs serrations are quickly filled with the squamous bone removed, which makes them blunt and less effective, resulting in the need to apply increasing pressure on the handpiece which in turn may contribute to excessive frictional heat produced during preparation, leading to post-surgical complications such as bone-necrosis, pain, retarded or prevented healing. 8 Although the use of rotating and low-frequency oscillating instruments deliver acceptable clinical results in the everyday surgical practice, their use is always highly traumatic on the microscopic, histologic and molecular level 8 and their correct application is highly related to the surgeon’s manual capabilities. Rotating burs and low-frequency oscillating saws historically were the only common devices available to cut bone tissue in oral and maxillofacial surgery. By the end of the last century, piezoelectric surgical devices 9,10 – well known in orthopedic surgery since 1974 10,11 - and lasers of different wavelengths (Ne:YAG 1064nm, Hol:YAG 1980nm, Er:YAG 2940nm, ErCr:YSGG 2780nm, CO 2 9400/10600nm, Excimer-UV 193nm) were introduced to clinical application also in oral and maxillofacial bone surgery. 12-17 While piezoelectric surgical tools were specifically and originally developed and designed to cut bone, lasers were used for soft-tissue cutting and only few possible clinical applications and indications are known and verified for osteotomies of jaw-bones and none in orthopedic surgery. 13-15 Any tissue-cutting laser (Light Amplification of Stimulated Emission of Radiation) produces a coherent light beam of a specific wavelength mostly in the infrared (=heating) spectrum of electromagnetic waves. This infrared “heat- beam” then causes a sudden circumscribed microscopic explosive boiling of cell- and interstitial tissue-liquids (known as “photoacoustic effect”), leading to a thermo- mechanical ablation of soft-tissues and in case of bone, dentin and enamel to the explosive ejection of mineral particles. Once the liquid of the targeted tissue is consumed and fully evaporated, the mere heat of the laser- beam causes heat-coagulation and shrinking of collagen- fibers similar to the electro-coagulation of tissues treated with electric knifes and coagulator-devices (“electrotomes”). As consequence, oral soft tissues - composed of approximately 70% watery liquid– endure laser-radiation longer than bone, which contains only approximately 22% watery liquids, before sudden exponential temperature- increase leads to heat-necrosis, microfractures and carbonization of bone and dentin (Fig.1). This physical mode of action of lasers on different types of body-tissues requires a profound knowledge and a diligent and long training of the surgeon for a safe application. Moreover, lasers must not be used in vicinity of delicate soft-tissue-structures such as sinus- membranes and infraorbital, mental and mandibular nerve since they do not provide any kind of cutting- depth-control, thus limiting their application in oral and maxillofacial bone-surgery significantly. Piezoelectric devices for bone-surgery-applications are based on the ultrasonic fast and precise unidirectional expansion and contraction of piezoelectric crystals when electric voltage is applied to them. 18,19 These precise oscillations at a rate of 27 – 32 KHz (27 thousand to 32 thousand unidirectional movements per second) are modulated in their amplitude and extend alternately from 30 – 60 micrometers to preserve the basic collagenous texture of bone (Fig.2). Obviously, these small and precise unidirectional movements at ultrasonic speeds do not cause any adverse torque-forces due to the physical inertia and thus allow the surgeon a pressure-free and precise guidance of the ultrasonic surgical working tip on and in the bone. A physical effect caused by well-constructed Piezotomes in liquid-containing tissues and surrounded by the cooling saline-solution-flow is the so called “hydrodynamic cavitation effect”, which cannot be seen with the unaided eye because of its speed. 20 Any solid body oscillating in liquids at ultrasonic speeds creates a partial vacuum on the opposite side of the direction of movement, decreasing the boiling-temperature of the liquid to room temperatures effecting into a strong and expanding cool gaseous “cushion” around the oscillating working-tip (Fig.3). (Fig. 1) Histology of enamel and dentin after Erb:YAG- laserbeam-impact (Trichrome staining) at 5 W/1µs/3 pulses (Fig. 2) A: Piezoelectric device “Piezotome II” (Satelec- ACTEON/France), B: frequency-modulation of piezoelectric devices for bone cutting to preserve the basic collagen-fiber structure (“Sharpey fibers” of bone

Upload: others

Post on 20-Jul-2020

5 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: Is Piezoelectric Surgery the New Gold-Standard in Oral ......Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery,

| 12 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 13 |

ABSTRACT

Mills, drills, burs and low-frequency oscillating saws were the only tools for bone-cutting for a long time and their use rarely questioned on their biological and physiological effects on bone. With the emergence of new technologies in bone-cutting - such as lasers and piezoelectric cutting devices - more and more experimental and clinical studies shed light on the differences in the bone healing-cycle when traditional instrumentation is compared to these new technologies.

A search in medical and biomedical databases was performed and molecular-biologic, micromorphologic, histologic, experimental and clinical comparative studies were selected, excluding case-reports and unclear methodology.Experimental and clinical findings then were summarized and compared against each other by their micromorphologic, histologic, biologic and clinical effects resulting in a comparative overview with impact to the clinician’s daily routine-work.

At the current state of secure knowledge, scientific literature suggests piezoelectric surgical tools for bone-cutting to provide the highest clinical precision, the least procedural bone-loss, an improved bone-healing and best “ease-of-use”.

KEYWORDS

Piezoelectric surgery, Piezosurgery, Piezotome-surgery, Ultrasonic bone cutting, Bone-cutting instruments, Osteotomy, Drill, Bur, Mill, Laser.

Is Piezoelectric Surgery the New Gold-Standard in Oral Surgery and Implantology?A Scientific Literature Review

Troedhan Angelo - MD, DMD, PhDSpecialist Craniomaxillofacial Surgery, Specialist Dentistry

V.Prof. Health Science University Vientiane – Lao PDR | Vienna, Austria | [email protected]

Ziad Tarek Mahmoud - DDS, MScLecturer of Oral and Maxillofacial Surgery, Faculty of Dentistry, Alexandria University | Alexandria, Egypt | [email protected]

INTRODUCTION

Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery, bone-management, guided bone regeneration (GBR), dental implant insertion, orthognathic surgery etc.) are performed traditionally with chisels, burs, osteotomes and low-frequency oscillating saws.

However, rotating or low-frequency oscillating instruments are very difficult to handle when used on cortical and trabecular bone due to its procedural high physical torque-moment. The result is a loss of tactile sensitivity due to the requirement of pressure on the hand-piece, difficulty in the determination of cutting depth and iatrogenic impairment in undesired areas due to a failure in the accurate adjustment of the speed of a rotating bur or oscillating saw, particularly when ultimate precise osteotomies are essential.1 When chisels or osteotomes are used, labyrinthine concussion of the inner ear, positional vertigo or even brain concussion may occur.2,3

Major and unavoidable medical drawbacks of the use of motorized rotary, reciprocal or low-frequency oscillating instruments in bone-cutting are enormous procedural bone losses, significant bone-necrosis due to overheating and the high risk of soft tissue injury to important anatomical structures such as the inferior alveolar nerve or maxillary sinus-membrane and deposition of metal shavings and bacterial contamination.4-7

Burs serrations are quickly filled with the squamous bone removed, which makes them blunt and less effective, resulting in the need to apply increasing pressure on the handpiece which in turn may contribute to excessive frictional heat produced during preparation, leading to post-surgical complications such as bone-necrosis, pain, retarded or prevented healing.8

Although the use of rotating and low-frequency oscillating instruments deliver acceptable clinical results in the everyday surgical practice, their use is always highly traumatic on the microscopic, histologic and molecular

level8 and their correct application is highly related to the surgeon’s manual capabilities. Rotating burs and low-frequency oscillating saws historically were the only common devices available to cut bone tissue in oral and maxillofacial surgery. By the end of the last century, piezoelectric surgical devices9,10 – well known in orthopedic surgery since 197410,11 - and lasers of different wavelengths (Ne:YAG 1064nm, Hol:YAG 1980nm, Er:YAG 2940nm, ErCr:YSGG 2780nm, CO2 9400/10600nm, Excimer-UV 193nm) were introduced to clinical application also in oral and maxillofacial bone surgery.12-17

While piezoelectric surgical tools were specifically and originally developed and designed to cut bone, lasers were used for soft-tissue cutting and only few possible clinical applications and indications are known and verified for osteotomies of jaw-bones and none in orthopedic surgery.13-15

Any tissue-cutting laser (Light Amplification of Stimulated Emission of Radiation) produces a coherent light beam of a specific wavelength mostly in the infrared (=heating) spectrum of electromagnetic waves. This infrared “heat-beam” then causes a sudden circumscribed microscopic explosive boiling of cell- and interstitial tissue-liquids (known as “photoacoustic effect”), leading to a thermo-mechanical ablation of soft-tissues and in case of bone, dentin and enamel to the explosive ejection of mineral particles. Once the liquid of the targeted tissue is consumed and fully evaporated, the mere heat of the laser-beam causes heat-coagulation and shrinking of collagen-fibers similar to the electro-coagulation of tissues treated with electric knifes and coagulator-devices (“electrotomes”). As consequence, oral soft tissues - composed of approximately 70% watery liquid– endure laser-radiation longer than bone, which contains only approximately 22% watery liquids, before sudden exponential temperature-increase leads to heat-necrosis, microfractures and carbonization of bone and dentin (Fig.1).

This physical mode of action of lasers on different types of body-tissues requires a profound knowledge and a diligent and long training of the surgeon for a safe application. Moreover, lasers must not be used in vicinity of delicate soft-tissue-structures such as sinus-membranes and infraorbital, mental and mandibular nerve since they do not provide any kind of cutting-depth-control, thus limiting their application in oral and maxillofacial bone-surgery significantly.

Piezoelectric devices for bone-surgery-applications are based on the ultrasonic fast and precise unidirectional expansion and contraction of piezoelectric crystals when electric voltage is applied to them.18,19 These precise oscillations at a rate of 27 – 32 KHz (27 thousand to 32 thousand unidirectional movements per second) are modulated in their amplitude and extend alternately from 30 – 60 micrometers to preserve the basic collagenous texture of bone (Fig.2).

Obviously, these small and precise unidirectional movements at ultrasonic speeds do not cause any adverse torque-forces due to the physical inertia and thus allow the surgeon a pressure-free and precise guidance of the ultrasonic surgical working tip on and in the bone. A physical effect caused by well-constructed Piezotomes in liquid-containing tissues and surrounded by the cooling saline-solution-flow is the so called “hydrodynamic cavitation effect”, which cannot be seen with the unaided eye because of its speed.20 Any solid body oscillating in liquids at ultrasonic speeds creates a partial vacuum on the opposite side of the direction of movement, decreasing the boiling-temperature of the liquid to room temperatures effecting into a strong and expanding cool gaseous “cushion” around the oscillating working-tip (Fig.3).

(Fig. 1) Histology of enamel and dentin after Erb:YAG-laserbeam-impact (Trichrome staining) at 5 W/1µs/3 pulses

(Fig. 2) A: Piezoelectric device “Piezotome II” (Satelec-ACTEON/France), B: frequency-modulation of piezoelectric devices for bone cutting to preserve the basic collagen-fiber structure (“Sharpey fibers” of bone

Page 2: Is Piezoelectric Surgery the New Gold-Standard in Oral ......Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery,

| 14 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 15 |

This is why cutting bone with Piezotomes is less a mechanical process like with burs, saws or chisels but more an atraumatic and microscopic precise separation of soft and hard-tissue layers by the separating pressurized gaseous “cushion” around the oscillating tip, gliding through the bone like a Hovercraft glides over water and similar to the photoacoustic effect created by infrared laser-beams. Contrary to laser-beams - which can only focus on one single microscopic spot at a time and which create microscopic gas-bubble-explosions at approximately 100°C or more (overheated gas) - Piezotome-working tips are flat-covered with gaseous bubbles at room-temperatures or below.21 (Fig.3)

Moreover, the hydrodynamic cavitation effect also causes less mechanical destruction of blood vessels compared to rotary instruments, which results in an almost bleeding-free surgical site. It also helps to maintain good visibility onto the surgical site by dispersing the coolant saline solution as an aerosol that causes the sparse blood to wash away.22 In addition, the hydrodynamic cavitation-effect inheres a disinfecting property similar to infrared lasers, attributed to the fragmentation of bacterial cell walls.23,24 A significant decrease of risk of intrasurgical dispersion of infections into the surrounding bone is the result compared to rotary instruments.6,7

BIOLOGIC & PHYSIOLOGIC EFFECTS OF PIEZOELECTRIC BONE-SURGERY APPLICATION

HISTOCHEMICAL EFFECTS OF APPLICATION OF ULTRASOUND ON SOFT AND HARD TISSUES

In general medicine, orthopedic surgery and traumatology the positive effects of ultrasound-application to significantly enhance healing processes

are well known since the last century. Ultrasound application enhances significantly the production of Interleukin 8 (IL8), Fibroblast Growth Factor (FGF) and Vascular Endothelial Growth Factor (VEGF), promoting a faster and richer vascularization of the healing site both in soft and hard tissues as well as the growth of the organ-specific collagenous fiber-pattern which is the very basic structure of every organ, also bone.25,26 Especially when applied to healing bone the application of ultrasound increases bone volume, osteoid thickness and mineral apposition rate significantly resulting in a better and faster bone-healing27 and is widely used in general medicine, orthopedic surgery and traumatology.

These proven physiologic facts were verified in experimental and clinical studies also for the application of Piezotome-surgical procedures in oral and maxillofacial surgery when compared to traditional procedures with rotary burs.28,29

HISTOLOGIC EFFECTS OF PIEZOELECTRIC SURGERY

A scanning electron microscopy study comparing the traditional Lindemann-bur with sonic and ultrasonic instrumentation revealed “the use of ultrasonic instruments to result in extremely precise cuts and reduced bone damage. Lindemann bur showed less precision and higher bone damage both in cortical and in cancellous bone. In cortical bone, ultrasonic and sonic cuts showed nicely opened bone vascular canals, while Lindemann bur showed many canals closed by abrasions, exfoliation and cracks by dragging attrition. In cancellous bone, ultrasonic cut showed intact trabeculae and trabecular spaces free of debris, while sonic cut showed more debris accumulation in trabecular spaces. Lindemann bur showed huge quantity of bone debris that filled trabecular spaces.”30

A direct ex vivo histologic comparison of bone healing after cutting bone with rotary burs and two different piezoelectric devices (Piezotome I and Piezotome II, both ACTEON/France) revealed a highly significant improvement of bone healing regarding bone-fill and bone mineral density in the osteotomy-gap in favor of both Piezotomes.31

Obviously, above all, the use of piezoelectric bone-cutting-devices macroscopically leads to significant less procedural bone-loss when compared to rotary instruments or lasers. (Fig. 4)

CLINICAL AND MEDICAL EFFECTS OF APPLICATION OF PIEZOELECTRIC SURGICAL TOOLS

PATIENT MORBIDITY

The TKW-Research-Group – specialized in clinical research and development of ultrasonic surgical tools and applications – were the first to investigate the practical clinical implications of the experimental results in a multicenter split-mouth study on the removal of impacted third molars.32

(Fig. 3) Depiction of a piezoelectric handpiece, its oscillation direction and the cycle of the hydrodynamic cavitation-effect, creating microbursts of gaseous bubbles on the surface of the working tip “BS 4” for Piezotomes

With the results of this first randomized clinical split-mouth study the authors were able to prove the enormous clinical impact the application of pure Piezotome-surgery provides for patients compared to the use of rotary instruments by a postsurgical reduction of swelling of more than 50% (Fig. 5) and pain (Fig. 6).The initially doubted results of this clinical study were later on verified by numerous clinical studies with the same or a similar study-design.33-37

SOFT TISSUE PRESERVATION

Prior to experimental and clinical studies in dentistry and oral and maxillofacial surgery neurosurgeons discussed and evaluated the superior preservation of critical soft tissues such as the spinal cord and brain tissue when

(Fig. 5) Comparative cumulative morbidity (swelling) when impacted third molars are removed either by drills/mills (red) or Piezotomes (green)

(Fig. 4) Comparative procedural bone-loss when harvesting autologous bone-blocks with different bone-cutting tools

(Fig. 6) Comparative cumulative morbidity (pain) when impacted third molars are removed either by drills/mills (red) or Piezotomes (green)

Page 3: Is Piezoelectric Surgery the New Gold-Standard in Oral ......Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery,

| 16 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 17 |

piezoelectric bone surgery is used38 with an ever increasing use of Piezotomes in spinal and skull-base surgery.

Mucoperiostal Flap Preparation

Every oral surgical procedure starts with the preparation of a mucoperiostal flap, up to now performed with conventional mucoperiostal elevators. What is often forgotten is the fact, that only a fully intact periosteum (as the sole carrier of bone-regeneration by osteoblast-induced mineralization of the initial callus-formation) provides the requisites for an undisturbed bone healing39 or a fast mineralization of the augmentation-site in GBR-procedures.40,41

Experimental research lately revealed a significant improvement of microcirculation in the periosteum and a significant higher functional capillary density after surgery when mucoperiostal flaps are prepared with piezoelectric devices in comparison to traditional mucoperiostal elevators.42 The experimental results later on were verified by microscopic clinical studies proving the clean and uninjured separation of the periosteum from the bone when Piezotome-surgical preparation is performed.43 (Fig.7)

Preservation of Critical Soft-Tissues in Dentistry, Oral and Maxillofacial Surgery

As mandatory in neurosurgery also the dentist and oral surgeon by all means has to preserve the full function of critical soft tissues such as the mandibular, mental and infraorbital nerve as well as the periosteum of the sinus-membrane for successful sinuslift-procedures.44,45

Comparative clinical studies (Piezotomes versus rotating burs, oscillating saws and chisels) proved the superior safety and preservation regarding an unaffected function of the mandibular/mental nerve in critical osteotomies adjacent to the mandibular nerve at removal of impacted third molars, cyst enucleation, complex osteotomies for bone-augmentation and in orthognathic surgery.33,34,46-48 (Fig. 8,9)

(Fig. 7) Reflected light microscopic image when Periosteum is detached by piezoelectric surgical devices at 20x magnification

(Fig. 8) Depiction of the fully intact mandibular nerve after engulfing cyst-tissue removal with Piezotome

(Fig. 9) Application of piezoelectric bone-cutting devices in orthognathic surgery (BSSO). A: lingual vertical osteotomy, B: mesio-distal ascending and distal buccal osteotomy, C: completed SSO with almost no bleeding

A

B C

The unpunctured/unruptured preservation of the periosteum of the sinus-membrane is mandatory for prevention of postsurgical infectious sinusitis49 and successful subantral mineralized bone-regeneration (sinuslift).44,50,51 Clinical studies prove piezoelectric devices with special diamond coated tip-designs to significantly reduce the incidence of accidental iatrogenic membrane perforations among surgeons with limited experience.52 With piezoelectric surgical procedures the perforation rate of the sinus-membrane can be reduced from 24 – 56%52-54 with rotary instruments to 5%55. (Fig. 10)

Mandibular Nerve/Inferior Alevolar Nerve - Lateralization (MNL/IANL)

A good alternative to vertical bone-augmentation in the lateral mandible (vertical bone distraction-osteogenesis, vertical block-grafting) - which in clinical studies and systematic reviews present a high short and long-term failure-rate56,57 – would be the mandibular nerve/inferior alveolar nerve-lateralization (MNL/IANL). However, due to the lack of a clear view onto the surgical site, the lack of depth control with rotary and/or low-frequency oscillating saws and the enormous risk to unrecoverable damage the mandibular nerve this procedure is rarely performed and if, only by highly experienced and specialized surgeons.58,59 With the introduction of piezoelectric surgical devices and clear surgical protocols the risk of a permanent damage of the mandibular nerve and the period of neurotmesis or hypaesthesia can be decreased significantly both by the excellent visibility of the surgical site, the perfect depth control of the

osteotomies and the superior soft tissue safety of piezo-surgical instruments.58,60 (Fig. 11)

Transcrestal Hydrodynamic Ultrasonic Cavitational Sinuslift (tHUCSL-INTRALIFT)

As further improvement both in ease of application, predictability and soft-tissue safety and as an alternative or entire replacement of lateral approach sinuslift the tHUCSL-INTRALIFT was introduced in 2007.61(Fig. 12)This minimal invasive transcrestal sinuslift-procedure (Fig. 13) allows a fully scalable subantral augmentation both for mesio-distal extension and augmentation-height in every anatomical situation (Fig. 14) and least possible complication rate (2,69%) as was proven in a prospective clinical multicenter-study.62

The high success-rate of the INTRALIFT also in the hand of the not so experienced surgeon is based mainly on the transcrestal approach with diamond coated ultrasonic tips and precise separation of the sinus-membrane from the bony antrum-floor by the cavitation effect,20,63 leaving

(Fig. 10) Lateral approach sinuslift with Piezotomes. A: preparation of the lateral bone window with a diamond-coated tip (SL 1) fully preserving the periostal layer of the sinus-membrane, B: initial detachment of the intact sinus-membrane with ultrasonic tip SL3, C: advancement of detaching the sinus-membrane with 90° angulated ultrasonic tip SL 4, D: advancement of detaching the sinus-membrane with 120° angulated ultrasonic tip SL 5

(Fig. 11) Mandibular nerve lateralization (MNL). A: CBCT presurgical situation, B: completed osteotomy and removal of the buccal cortical plate covering the mandibular nerve, C: removal of bony spikes with diamond-coated ball-shaped ultrasonic tip SL 2 prior to nerve-lateralization, D: fully intact mandibular nerve lateralized, E: implant insertion, F: CBCT-scan after 3 months

Page 4: Is Piezoelectric Surgery the New Gold-Standard in Oral ......Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery,

| 18 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 19 |

(Fig. 12) Surgical-protocol-scheme of transcrestal Hydrodynamic Ultrasonic CavitationalSinus Lift (tHUCSL-Intralift, 2-stage surgery). A: minimal invasive crestal flap, B: opening of the bony sinus-floor with diamond-coated ultrasonic tip TKW 2, C: preparation of the receptacle for TKW 5 tip with diamond-coated TKW 4-tip, D: hydrodynamic-hydraulic separation of the sinus-membrane from the bony antrum-floor with TKW 5-tip, utilizing the cavitation effect, E: application of bone-graft material through the 3mm-diameter osteotomy (if primary stability can be achieved by an individual implant system, implant can be inserted simultaneously), F: in cases of 2nd-stage implant insertion implant-site-preparation with drills or ultrasonic tips, G: primary stable implant insertion

the osteogenic periosteal layer of the sinus-membrane fully intact and functional.43-45

Even in case of an iatrogenic puncture of the sinus-membrane at preparation of the transcrestal approach, the INTRALIFT mostly can be performed successfully without further rupture of the sinus-membrane.64

Meanwhile – because of its predictability, ease of application and standardized surgical protocol – the tHUCSL-INTRALIFT is established as sinuslift-procedure of choice in clinical biomaterial and implant-research.65,66

Piezotome Enhanced Subperiostal Tunnel Technique

By its clean and predictable separation of periosteum from bone, keeping the full osteogenic potential of the periosteum intact, lately a well-known technique for vertical and lateral bone-augmentation was revived: the subperiostal tunnel technique,67-69 again yielding predictable and constant results and least risk of failure.43 (Fig. 15)

HARD TISSUE PRESERVATION

As depicted in Fig. 4, piezoelectric ultrasonic bone-cutting allows highest possible precision in design and depth of any kind of osteotomy with proven significant advantages regarding atraumaticity, speed and predictability of bone healing.11,18,22,27-31 By its explicit precision piezoelectric surgical tools allow a significant expansion of existing

(Fig. 14) Panoramic radiographic follow up of INTRALIFT-case. A: presurgical state, B: postsurgical state (white arrow marks the INTRALIFT osteotomy-site), C: implant insertion, D: state before implant loading after 6 months

A

B

D

C

(Fig. 13) Typical tHUCSL-INTRALIFT-site. A: minimal invasive crestal flap, INTRALIFT osteotomy, INTRALIFT and bone application completed, B: wound closure

BA

surgical protocols in bone-surgery which were restricted to certain bone-dimensions due to the massive procedural bone-loss with rotary instruments until now.

Routine Oral Surgical Procedures (impacted tooth removal, apisectomies)

Due to its property to cut bone almost lossless and with precise depth control piezoelectric bone-surgery allows anatomy-preserving surgical procedures. Whereas in most surgeries of removal of impacted third molars tremendous amounts of bone get lost by milling with burs, pure piezoelectric-surgical removal without use of any burs allows a full restitution of the patients anatomy at the end of the surgical procedure avoiding large long-term bone defects in the retromolar area which might cause unpleasant food-retention at chewing. (Fig. 16)

Defective healing of the alveolar crest after apisectomies with rotating burs – especially in the upper front, when

later on the tooth finally has to be extracted and a dental implant inserted – is very common. Following a piezo-surgical protocol with an anatomical correct full reconstruction of the resection-site, defective healing can be avoided. When later on the tooth might have to be removed, no additional buccal bone-augmentation procedures will be necessary. (Fig. 17)

Autologous Bone-block Transplants

Since possible geometric dimensions and anatomic locations restrict intraoral bone-block harvesting, procedural bone-loss in most cases is unacceptable and might lead to insufficient augmentations. Since with piezoelectric bone-scalpels almost no procedural bone-loss occurs, piezoelectric bone harvesting should be preferred. (Fig. 4)

(Fig. 15) Mandibular nerve Piezotome enhanced subperiostal tunnel technique (PeSTT). A: preparation of the subperiostal tunnel with a modified BS 4-tip, B: subperiostal tunnel-preparation completed with almost no bleeding, leaving the periosteum fully intact, C: application of a self hardening biphasic bone graft biomaterial (easygraft

Crystal/GUIDOR-SUNSTAR/Switzerland), D: augmentation with easygraft completed, the biomaterial is hardened, immobilized and acts like an autologous bone-block, E: CAT-scan after 8,5 months (single-piece Q1-implant (TRINON/Germany) immediately loaded)

(Fig. 16) Piezoelectric surgical removal of an impacted third molar with a coronary cyst engulfing the mandibular nerve. A: presurgical panoramic X-ray, B: bone-lossless preparation of a lateral mandibular bone window(similar to autologous bone-block preparation) with BS 5 “bone-scalpel” for Piezotomes, C: mobilization of impacted third molar with ultrasonic ligament-cutter-tips, D: forceless removal of impacted third molar, E: anatomical correct reconstruction of the mandibular angle with the harvested autologous bone-block (due to almost lossless bone-cut no fixation with osteosynthesis screws is necessary), F: panoramic X-ray after 6 months: bone healing is completed with no anatomical defects

E

Page 5: Is Piezoelectric Surgery the New Gold-Standard in Oral ......Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery,

| 20 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 21 |

Piezotome-enabled Distraction Osteogenesis

As reported the main cause of failure in distraction osteogenesis is based on the primary osteotomy,71 which with rotating and/or low-frequency oscillating instruments does not allow a perfect osteotomy-design as well as leads to the common problems known for application of these instruments on and in bone.4-8 On the other side, piezoelectric surgical instrumentation fully avoids these obstacles and allows a perfect and individual design of the bone-block to be distracted. (Fig. 18) Nevertheless, further clinical studies have to be undertaken with higher patient numbers to support this assumption.

Vertical Alveolar Crest-Split & Horizontal Distraction

One of the most common diagnosis in the course of dental implant planning is an insufficient alveolar crest-width due to the natural and/or iatrogenic lateral atrophy of the alveolar ridge.72-74 Lateral appositional

bone-block grafting or - nowadays - the Piezotome enhanced subperiostal tunnel technique43 allow to increase the width of the alveolar crest but need to be performed in two stages with longer healing periods.43

Similar to procedures in distraction osteogenesis the narrow alveolar crest can be split vertically and distracted horizontally, mostly with simultaneous implant-insertion, but was limited to crest-width of minimum 3-4mm due to the procedural bone loss with rotating burs or mills and the need to prepare a mucoperiostal flap.75 Due to the lack of depth-control and imprecision of the vertical osteotomy with rotary instruments accidental fractures of the distracted buccal compacta were reported76 as well as secondary vertical bone loss of 3mm or more in the healing-period.76

With the Piezotome enabled flapless vertical alveolar crest-split and horizontal distraction the indication for crest-splitting can be narrowed down to alveolar crest widths of 1mm.75,76 (Fig. 19,20)

Monocortical Interproximal Alveolar Ridge Corticotomy for Orthodontic Teeth Movement Acceleration

Orthodontic tooth movement is impaired by the physical presence of the coronary alveolar cortical bone surrounding the neck of the root. Therefore, it becomes necessary to disrupt this cortical bone via surgery to allow a faster orthodontic tooth movement. Following periodontal accelerated osteogenic orthodontics (PAOO) by corticotomy, a transient decalcification-recalcification process of the alveola occurs speeding up the orthodontic movement of teeth.77,78 Nevertheless, this surgical technique – performed with rotary instruments

(Fig. 17) Typical apisectomy with Piezotomes. A: periosteum-preserving preparation of mucoperiostal flap with BS 4-tip for Piezotomes, B: almost lossless osteotomy of buccal alveolar lamella, C: buccal alveolar lamella dislocated to approach apex, D: apisectomy with “Ninja” ultrasonic tip for Piezotomes, E: surgical site after degranulation with BS 4-tip and retrograde preparation of new apex with CE 1-tip, F: full reconstruction of local anatomy by repositioning of the buccal alveolar lamella leaving no apical bone-defect

E

(Fig. 18) Distraction osteogenesis-procedure with ultrasonic surgical devices. A: precise osteotomy of the alveolar-crest region to be distracted, B: control of proper bone-block-mobility (bone-block stays attached to periosteum palatal and coronar and therefore fully vital), C: distractor device attached, D: distraction completed, implant insertion with 120° angulated ultrasonic tip SL 5

- needs the reflection of a full thickness mucoperiostal flap, which leads to uncontrolled resorptions and cosmetic deficiencies in the healing period and is very traumatic both for soft and hard tissues as well as for the patient. Therefore, this surgical procedure is at least questionable from the medical standpoint.

With the introduction of the Piezocision-technique now an almost atraumatic buccal corticotomy without reflection of a mucoperiostal flap can be performed79-81 providing all advantages for a precise and rapid orthodontic tooth movement while completely lacking the medical and esthetic disadvantages. The risk of lesions of the adjacent periodontal ligaments is considered very low due to the design of the Piezocision-tips for Piezotomes. In case of need of buccal augmentation, the Piezotome enhanced subperiostal tunnel technique can be performed additionally.43 (Fig. 21)

Implant Site Preparation

Rotary drills remove bone and even when used at slow

speeds inhere the risk of damage in the trabecular bone.4-8 Repeated use of implant-drills lead to bluntness

of the drills and therewith to higher necessary loads on the handpiece, which then might lead to unwanted thermal effects in the bone.82

(Fig. 19) Surgical protocol of the Flapless Piezotome enhanced Crest Split and Widening (FPeCSW). A: lateral view of anatomical prerequisites for FPeCSW-procedure, B: depiction of the achievable postsurgical result

(Fig. 20) Clinical case following the surgical protocol for Piezotome-enhanced flapless Crest-Split (initial alveolar crest-width: 1mm). A: initial top-crestal mesio-distal mucoperiostal incision. No mucoperiostal flap is prepared, B: initial bone-lossless vertical mesio-distal osteotomy with CS 1 tip, C: initial horizontal distraction with CS 2 tip to allow easy vertical approach for next surgical step, D: mesial and distal buccal relief-osteotomies from inside of the mesio-distal osteotomy in buccal direction, E: step by step horizontal distraction of completed osteotomy with CS 4, 5 and 6 tip to a gap-width of 4mm. the buccal-distracted compact-bone stays fully attached to the periosteum and therewith fully vital, F: state after implant-insertion (Q2-implant) and gap-filling with self hardening biphasic bone-graft biomaterial (easygraft), G: crestal-gingival anatomy mostly allows gap-free tensionless wound-closure, H: final prosthetic result after 3,5 months

E

Page 6: Is Piezoelectric Surgery the New Gold-Standard in Oral ......Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery,

| 22 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 23 |

Experimental assessment of differences between the use of piezoelectric surgery and a conventional drill concerning neo-osteogenesis and inflammatory reaction after implant-site preparation revealed more newly formed bone with an increased amount of osteoblasts to be visible on the piezoelectric implant site during the early phase (7–14 days). The investigated humoral factors BMP-4, TGF-ß2 and IL-10 were increased in the piezoelectric group, while IL-1ß and TNFß were not. In conclusion, the piezoelectric device stimulated peri-implant osteogenesis, and a reduction of proinflammatory cytokines.83-85

Therefore, implant-site preparation with piezoelectric surgical devices seems favorable to achieve clinically high primary implant-stability and an enhanced and safer osseointegration. (Fig. 22)

PIEZOTOME-APPLICATIONS IN THE DAILY DENTAL ROUTINE

TOOTH REMOVAL

Once all treatment possibilities to preserve a tooth are exhausted, finally teeth will have to be removed and possibly to be replaced by dental implants. Fractured teeth, roots and ankylosed teeth challenge the dentist to preserve the alveolar bone height and width for a planned later implant insertion. When drills, burs and periotomes have to be used, mostly part of the alveolar ridge has to be sacrificed in order to remove the tooth or its fragments, leading to the need for bone augmentation to enable implant insertion.

An alternative to bone-destructive tooth-removal now is piezoelectric tooth-removal with piezoelectric ligament-cutters. Similar to the preparation of a mucoperiostal flap with Piezotomes43,45 – the periodontal ligament consists of the same Sharpey-fibers attaching the tooth to the bone as the periosteum is attached to the bone86 – the periodontal ligament is precisely cut and the periodontal gap widened by the cavitation-effect without bone-loss, enabling an almost forceless removal of the tooth. (Fig. 23)

CROWN LENGTHENING

In case the root of a tooth with crestal caries or intrabony crestal fracture can still be treated with a pin and a crown, a perfect margin has to be prepared for later impression. In case of crestal caries or a subcrestal fracture, crestal bone has to be removed carefully to allow the later preparation of the crown margins. The minimal invasive removal of crestal bone with rotary instruments challenges the dentist. As a better alternative nowadays, piezoelectric instruments should be used for this task especially in the early period of gaining manual experience. (Fig. 24)

(Fig. 22) Implant-site preparation with ultrasonic-tips. A: initial pilot-preparation, B: widening of the osteotomy to the planned implant-diameter with ultrasonic tips, C: implant-site-preparation finished, D: primary stable implant insertion, E: control X-ray after implant insertion

(Fig. 21) Piezocision-technique for improved orthodontic tooth-movement. A: flapfreeinterradicular mucoperiostal incision, B: incision-patern in a typical case, C: flapfreemonocortical osteotomy with Piezocision-tips for Piezotomes, D: if needed, after Piezocision-procedure is completed a subperiostal tunnel can be prepared for buccal bone-augmentation

DISCUSSION

Obviously, the use of Piezotomes in the daily dentists as well as oral and maxillofacial surgeons´ clinical routine seems to be preferable since common disadvantages of the use of rotary mills and burs, low-frequency oscillating saws and chisels as well as lasers can be avoided. The thorough and deep research of the published literature did not reveal any hint of possible disadvantages of Piezotomes in the clinical routine, on histologic and on micromorphologic level. Contrary, the use of piezoelectric surgical instrumentation seems to provide significant advantages for both the dentist/surgeon and their patients, achieving higher and predictable success-rates in any kind of bone-surgery and less patient-morbidity.32-36 Furthermore, some highly invasive and

manually challenging traditional surgical techniques can now be replaced completely by easy to learn and minimal invasive piezosurgical techniques.

One major criticism of piezoelectric surgery – the higher time-consumption for piezoelectric surgeries – was already disproved ten years ago in a prospective multicenter study.32 After a learning curve with higher time-consumption for routine-surgeries – which is common for any kind of new surgical technique to be learned by dentists and surgeons – time-spans for these procedures go back to normal level or even less. With the introduction of devices with higher power-output (average output: 25-30 W, top-device: 60 W) surgery-times are further reduced.

CONCLUSIONS

In the light of this thorough literature-research and comparison to rotary mills, burs, low-frequency oscillating saws, chisels and infrared lasers Piezotome-surgery seems to outclass traditional instruments and lasers by far on clinical, histologic and micromorphologic level providing precise osteotomies, maximum soft-tissue preservation and improved bone-healing even in the surgical beginners hand and by this seems to be the new gold-standard in dentistry, oral and maxillofacial surgery.

REFERENCES1. Giraud JY, Villemin S, Darmana R, Cahuzac JP, Autefage A,

Morucci JP. Bone cutting. Clin Phys PhysiolMeas. 1991;12:1-19.2. Flanagan, DF. Labyrinthine concussion and positional vertigo after

osteotome site preparation. Implant Dent. 2004;13:129-32.3. Chiarella G, Leopardi G, De Fazio L, Chiarella R, Cassandro

E.Benign paroxysmal positional vertigo after dental surgery. Europ Arch OtoRhinoLaryng. 2008;265(1):119-22.

4. Siegel S, Fraunhofen A. Irrigation solution and pressure effects on tooth sectioning with surgical burs. Oral Surg Oral Med Oral Path Oral RadiolEndod. 1999;87:552-6.

5. Toksvic L, Ryd L, Lindstrand A. On the problem of heat generation in bone cutting: Studies on the effects of liquid cooling. J Bone Joint Surg. 1991;73:13-5.

6. Eriksson AR, Albrektsson T, Albrektsson B. Heat caused by drilling cortical bone. Temperature measured in vivo in patients and animals. ActaOrthopScand. 1984;55:629-31.

7. Matthews LS, Hirsch C. Temperatures measured in human cortical bone when drilling. J Bone Joint Surg Am. 1972;54(2):297-308.

8. Allan W, Williams ED, Kerawala CJ. Effects of repeated drill use on temperature of bone during preparation for osteosynthesis self-tapping screws. Br J Oral MaxillofacSurg. 2005;43:314-9.

9. Vercellotti T. Technological characteristics and clinical indications of piezoelectric bone surgery. Minerva Stomatol. 2004;53:207-14.

10. Volkov MV, Shepeleva IS. The use of ultrasonic instrumentation for the transection and uniting of bone tissue in orthopaedic surgery. ReconstrSurgTraumatol. 1974;14:147-52.

11. Aro H, Kallioniemi H, Aho AJ, Kellokumpu-Lehtinen P. Ultrasonic device in bone cutting. A histological and scanning electron microscopical study. ActaOrthopScand. 1981;52:5-10.

12. Kautzky M, Troedhan A, Susani M, Schenk P. Infrared laser stapedotomy. Europ Arch OtoRhinoLaryng. 1991;248(8):449-51.

13. Stubinger S, Ghanaati S, Saldamli B, Kirkpatrick CJ, Sader R. Er:YAG laser osteotomy: preliminary clinical and histological results of a new technique for contact-free bone surgery. EurSurg Res. 2009;42:150-6.

(Fig. 23) Tooth removal with ultrasonic ligament-cutters for Piezotomes. A: removal of an ankylosed root-fragment with LC 1-tip, B: removal of a fractured root-fragment with LC 2-tip,

C: fragmentation of a root-fragment with Ninja-tip to allow dislocation from underneath a bridge, leaving the bridge intact

(Fig. 24) Surgical Crown Extension to allow sufficient supracrestal crown-margin-preparation and impression-taking

Page 7: Is Piezoelectric Surgery the New Gold-Standard in Oral ......Surgical procedures in dentistry and Oral and Maxillofacial surgery (such as tooth removal, apisectomies, periodontal surgery,

| 24 | Smile Dental Journal | Volume 11, Issue 4 - 2016 Smile Dental Journal | Volume 11, Issue 4 - 2016 | 25 |

14. Hibst R, Keller U. Experimental studies of the application of the Er: YAG laser on dental hard substances: I. Measurement of the ablation rate. Lasers Surg and Med. 1989;9(4):338-44.

15. Hibst R, Keller U. Experimental studies of the application of the Er: YAG laser on dental hard substances: II. Measurement of the ablation rate. Lasers Surg and Med. 1989;9(4):345-51.

16. Wigdor HA, Walsh JT, Featherstone JDB, Visuri SR, Fried D, Waldvogel JL. Lasers in dentistry. Lasers Surg Med. 1995;16:103-33.

17. Miserendino LJ. The laser apicoectomy: endodontic application of the CO2 laser for periapical surgery. Oral Surg Oral Med Oral Pathol. 1988;66(5):615-9.

18. Yaman Z, Suer BT. Piezoelectric surgery in oral and maxillofacial surgery. Ann Oral MaxillofacSurg. 2013;1:1-9.

19. Stübinger S, Kuttenberger J, Filippi A, et al. Intraoral piezosurgery: preliminary results of a new technique. J Oral MaxillofacSurg. 2005;63:1283-7.

20. Moholkar VS, Pandit AB. Bubble behavior in hydrodynamic cavitation: Effect of turbulence. AIChE J. 1997;43:1641–8.

21. Vyas N, Pecheva E, Dehghani H, Sammons RL, Wang QX, Leppinen DM, Walmsley AD. High Speed Imaging of Cavitation around Dental Ultrasonic Scaler Tips. PloS one. 2016;11(3):e0149804.

22. Gupta SJ, Gupta A, Gautam V, Nangia R, Verma P. Stipulative interdisciplinary approach of piezosurgery in modern dentistry. J Pharm Bio med Sci. 2015;5:624-31.

23. Nalbandian S. Piezosurgery techniques in Implant Dentistry. Aust Dent Pract. 2011;116-26.

24. Walmsley AD, Laird WR, Williams AR. Dental plaque removal by cavitational activity during ultrasonic scaling. J ClinPeriodontol. 1988;15:539-43.

25. Reher P, Doan N, Bradnock B, Meghji S, Harris M. Effect of ultrasound on the production of IL-8, basic FGF and VEGF. Cytokine. 1999;11(6):416-23.

26. Suchkova VN, Baggs RB, Sahni SK, Francis CW. Ultrasound improves tissue perfusion in ischemic tissue through a nitric oxide dependent mechanism. ThrombHaemost. 2001;88(5):865-70.

27. Rutten S, Nolte PA, Korstjens CM, van Duin MA, Klein-Nulend, J. Low-intensity pulsed ultrasound increases bone volume, osteoid thickness and mineral apposition rate in the area of fracture healing in patients with a delayed union of the osteotomized fibula. Bone. 2008;43(2):348-54.

28. Simonetti M, Facco G, Barberis F, Signorini G, Capurro M, Rebaudi A, Sammartino G. Bone characteristics following osteotomy surgery: an in vitro SEM study comparing traditional Lindemann drill with sonic and ultrasonic instruments. Periodontol Oral SurgEsthImpl Dent O. 2013;1(3):187-93.

29. Mouraret S, Houschyar KS, Hunter DJ, Smith AA, Jew OS, Girod S, Helms JA. Cell viability after osteotomy and bone harvesting: comparison of piezoelectric surgery and conventional bur. Int J Oral MaxFacSurg. 2014;43(8):966-71.

30. Simonetti M, Facco G, Barberis F, Signorini G, Capurro M, Rebaudi A, Sammartino G. Bone characteristics following osteotomy surgery: an in vitro SEM study comparing traditional Lindemann drill with sonic and ultrasonic instruments. Poseido. 2013;1(3):187-94.

31. Reside J, Everett E, Padilla R, Arce R, Miguez P, Brodala N, Nares S. In vivo assessment of bone healing following piezotome® ultrasonic instrumentation. ClinImpl Dent Rel Res. 2015:17(2);384-94.

32. Troedhan A, Kurrek A, Wainwright M. Ultrasonic piezotome surgery: is it a benefit for our patients and does it extend surgery time? A retrospective comparative study on the removal of 100 impacted mandibular 3rd molars. Open J Stomatol. 2011;1(04):179.

33. Pappalardo S, Guarnieri R. Randomized clinical study comparing piezosurgery and conventional rotatory surgery in mandibular cyst enucleation. J CranioMaxFac Surg. 2014;42(5):e80-e85.

34. Goyal M, Marya K, Jhamb A, Chawla S, Sonoo PR, Singh V, Aggarwal A. Comparative evaluation of surgical outcome after removal of impacted mandibular third molars using a Piezotome or a conventional handpiece: a prospective study. Brit J Oral MaxFac Surg. 2012;50(6):556-61.

35. Piersanti L, Dilorenzo M, Monaco G, Marchetti C. Piezosurgery or conventional rotatory instruments for inferior third molar extractions? J Oral MaxFacSurg. 2014:72(9);1647-52.

36. Mantovani E, Arduino PG, Schierano G, Ferrero L, Gallesio G, Mozzati M,Carossa S. A Split-Mouth Randomized Clinical Trial to Evaluate the Performance of Piezosurgery Compared With Traditional Technique in Lower Wisdom Tooth Removal. J Oral MaxFacSurg. 2014;72(10):1890-7.

37. Gao Y, Lin Z, Rodella L F, Buffoli B, Wu X, & Zhou Y. Piezoelectric ultrasonic bone surgery system in the extraction surgery of supernumerary teeth. Journal of Cranio-Maxillofacial Surgery. 2014;42(8):1577-82.

38. Schaller BJ, Gruber R, Merten HA, Kruschat T, Schliephake H, Buchfelder M, Ludwig HC. Piezoelectric bone surgery: a revolutionary technique for minimally invasive surgery in cranial base and spinal surgery? Technical note. Neurosurg. 2005;57(4);E410.

39. Ozcelik D, Turan T, Kabukcuoglu F, Ugurlu K, Ozturk O, Basak M, Bankaoglu M. Bone induction capacity of the periosteum and neonatal dura in the setting of the rat zygomatic arch fracture model. Arch Fac Plastic Surg. 2003;5(4):301-8.

40. Malizos KN, Papatheodorou LK. The healing potential of the periosteum: molecular aspects. Injury. 2005:36(3);S13-S19.

41. Mahajan A. Periosteum: a highly underrated tool in dentistry. Int J Dent. 2012:717816. doi:10.1155/2012/717816.

42. Stoetzer M, Felgenträger D, Kampmann A, Schumann P, Rücker M, Gellrich NC, von See C. Effects of a new piezoelectric device on periosteal microcirculation after subperiosteal preparation. Microvasc Res. 2014;94:114-8.

43. Troedhan A, Wainwright M, Kurrek A, Schlichting I. Biomechanical stability of dental implants in augmented maxillary sites: results of a randomized clinical study with four different biomaterials and PRF and a biological view on guided bone regeneration. BioMed Res Int. 2015:17. doi:10.1155/2015/850340

44. Troedhan A, Kurrek A, Wainwright M.Biological Principles and Physiology of Bone Regeneration under the Schneiderian Membrane after Sinus Lift Surgery: A Radiological Study in 14 Patients Treated with the Transcrestal Hydrodynamic Ultrasonic Cavitational Sinus Lift (Intralift). Int J Dent. 2012:12. doi:10.1155/2012/576238

45. Troedhan A, Kurrek A, Wainwright M, Jank S, Schneiderian Membrane Detachment Using transcrestal Hydrodynamic Ultrasonic Cavitational Sinuslift: A Human Cadaver Head Study and Histology Analysis. J Oral MaxFacSurg. 2014;72(8):1503.

46. Pavlíková G, Foltán R, Horká M, Hanzelka T, Borunská H, & Šedý J. Piezosurgery in oral and maxillofacial surgery. International journal of oral and maxillofacial surgery. 2011:40(5):451-7.

47. Degerliyurt K, Akar V, Denizci S, & Yucel E. Bone lid technique with piezosurgery to preserve inferior alveolar nerve. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2009;108(6):e1-e5.

48. Landes C, Tran A, Ballon A, Santo G, Schübel F, & Sader, R. Low to high oblique ramus piezoosteotomy: A pilot study. Journal of Cranio-Maxillofacial Surgery. 2014;42(6):901-9.

49. Al-Dajani M. Recent trends in sinus lift surgery and their clinical implications. Clin Implant Dent Relat Res. 2014;18:204-12.

50. Srouji S, Kizhner T, David DB, Riminucci M, Bianco P, Livne E. The Schneiderian membrane contains osteoprogenitor cells: in vivo and in vitro study. CalcTissInt. 2009;84(2):138-45.

51. Berbéri A, Al-Nemer F, Hamade E. et al. Mesenchymal stem cells with osteogenic potential in human maxillary sinus membrane: an in vitro study. Clin Oral Invest. 2016; doi:10.1007/s00784-016-1945-6.

52. Seoane J, López-Niño J, García-Caballero L, Seoane-Romero JM, Tomás I, Varela-Centelles P. Membrane perforation in sinus floor elevation – piezoelectric device versus conventional rotary instruments for osteotomy: an experimental study. Clin Implant Dent Relat Res. 2013;15:867-73.

53. Toscano NJ, Holtzclaw D, Rosen PS. The effect of piezoelectric use on open sinus lift perforation: a retrospective evaluation of 56 consecutively treated cases from private practices. J Periodontol. 2010;81:167-71.

54. Kasabah S, Krug J, Simunek A, Lecaro MC: Can we predict maxillary sinus mucosa perforation? ActaMedica (Hradec Kralove). 2003;46:19.

55. Vercellotti T, De Paoli S, Nevins M. The piezoelectric bony window osteotomy and sinus membrane elevation: introduction of a new technique for simplification of the sinus augmentation procedure. Int J Periodontics Restorative Dent. 2001;21:561-7.

56. Enislidis G, Fock N, Millesi-Schobel G, Klug C, Wittwer G, Yerit K, Ewers R. Analysis of complications following alveolar distraction osteogenesis and implant placement in the partially edentulous mandible. Oral Surg Oral Med Oral Pathol Oral RadiolEndodont. 2005;100(1):25-30.

57. Esposito M, Grusovin MG, Felice P, Karatzopoulos G, Worthington HV, Coulthard P. The efficacy of horizontal and vertical bone augmentation procedures for dental implants-a Cochrane systematic review. Eur J Oral Implantol. 2009;2(3):167-84.

58. Bovi M. Mobilization of the inferior alveolar nerve with simultaneous implant insertion: a new technique. Case report. Int J Perio Rest Dent. 2005;25(4).

59. Vetromilla BM, Moura LB, SonegoCL, Torriani MA, Chagas OL. Complications associated with inferior alveolar nerve repositioning for dental implant placement: a systematic review. Int J Oral MaxFacSurg. 2014;43(11):1360-6.

60. De Vicente JC, Pena I, Brana P, Hernandez-Vallejo G. The use of piezoelectric surgery to lateralize the inferior alveolar nerve with simultaneous implant placement and immediate buccal cortical bone repositioning: a prospective clinical study. Int J Oral MaxillofacSurg. 2016;16:46-56.

61. Wainwright M, Troedhan A, Kurrek A. The IntraLift: A new minimal invasive ultrasonic technique for sinus grafting procedures. Implants. 2007;3:30-4.

62. Troedhan A, Kurrek A, Wainwright M, Schlichting I, Fischak-Treitl B, Ladentrog M. The transcrestal hydrodynamic ultrasonic cavitational sinuslift: Results of a 2-year prospective multicentre study on 404 patients, 446 sinuslift sites and 637 inserted implants. Open JStomatol. 2013;3:471-85.

63. Troedhan A, Kurrek A, Wainwright M, Jank S. Hydrodynamic Ultrasonic Sinus Floor Elevation - An Experimental Study in Sheep. J Oral MaxFacSurg. 2010;68(5):1125-30.

64. Jank S, Kurrek A, Wainwright M, Bek VE, Troedhan A. Rupture length of the sinus membrane after 1.2 mm puncture and surgical sinus elevation: an experimental animal cadaver study. Oral Surg Oral Med Oral Pathol Oral RadiolEndod. 2011;112(5);568-72.

65. Wainwright M, Torres-Lagares D, Pérez-Dorao B,Serrera-Figallo MA, Gutierrez-Perez JL, Troedhan A, Kurrek A. Histological and histomorphometricstudy using an ultrasonic crestal sinus grafting procedure. A multicentercase study. Med Oral Patol Oral Cir Bucal. 2016; doi:10.4317/medoral.20994.

66. Troedhan A, Schlichting I, Kurrek A, Wainwright M. Primary implant stability in augmented sinuslift-sites after completed bone regeneration: a randomized controlled clinical study comparing four subantrally inserted biomaterials. Nature Sci Rep. 2014;4:5877.

67. Rothstein SS, Paris DA, Zacek MP. Use of hydroxylapatite for the augmentation of deficient alveolar ridges. J Oral MaxFacSurg. 1984;42(4):224-30.

68. Propper RH. A technique for controlled placement of hydroxylapatite over atrophic mandibular ridges. J Oral MaxFacSurg. 1985;43(6):469-70.

69. Härle F, Kreusch T. Augmentation of the alveolar ridges with hydroxylapatite in a Vicryl tube. Int J Oral MaxFacSurg. 1991;20(3):144-8.

70. Douthitt JC, Gutmann JL, Witherspoon DE. Histologic assessment of healing after the use of a bioresorbable membrane in the management of buccal bone loss concomitant with periradicular surgery. J Endodont. 2001;27(6):404-10.

71. Saulacic N, Zix J, Iizuka T. Complication rates and associated factors in alveolar distraction osteogenesis: a comprehensive review. Int J Oral MaxFacSurg. 2009;38(3):210-7.

72. Cawood JI, Howell RA. A classification of the edentulous jaws. Int J Oral MaxFacSurg. 1988;17(4):232-6.

73. Araújo MG, Lindhe J. Dimensional ridge alterations following tooth extraction. An experimental study in the dog. J ClinPeriodontol. 2005;32(2):212-8.

74. Graves DT, Li J, Cochran DL. Inflammation and uncoupling as mechanisms of periodontal bone loss. J Dent Res. 2011;90(2):143-53.

75. Troedhan A, Kurrek A, Wainwright M, Schlichting I. The Piezotome-enabled flapless vertical alveolar crest-split and horizontal distraction. Results of a prospective comparative 3-year clinical multicenter-study with 239 patients, 261 crest-split sites and 488 inserted dental implants. Open J Stomatol. 2015;5:159-78.

76. Troedhan A, Kurrek A, Wainwright M. Vertical alveolar crest split and widening–an experimental study on cow ribs, ultrasonic tool development and test on human cadaver heads. Surg Tech Dev. 2012;2(1):10.

77. Wilcko W, Wilcko MT. Accelerating tooth movement: the case for corticotomy-induced orthodontics. Am J OrthodontDentofacOrthoped. 2013;144(1):8.

78. Wilcko MT, Wilcko WM, Pulver JJ, Bissada NF, Bouquot JE. Accelerated osteogenic orthodontics technique: a 1-stage surgically facilitated rapid orthodontic technique with alveolar augmentation. J Oral MaxFacSurg. 2009;67(10):2149-59.

79. Dibart S, Keser EI. Piezocision™: Minimally invasive periodontally accelerated orthodontic tooth movement procedure. Orthodontically Driven Corticotomy: Tissue Engineering to Enhance Orthodontic and Multidisciplinary Treatment, Wiley Blackwell (Publisher). 2014:119.

80. Dibart S, Surmenian J, DavidSebaoun J, Montesani L. Rapid treatment of Class II malocclusion with piezocision: two case reports. Int J PeriodontRestor Dent. 2010;30(5):487.

81. Dibart S, Keser E, Nelson D. Piezocision™-assisted orthodontics: Past, present, and future. Seminars in Orthodontics. 2015;21(3):170-5.

82. Stelzle F, Frenkel C, Riemann M, Knipfer C, Stockmann P, Nkenke E. The effect of load on heat production, thermal effects and expenditure of time during implant site preparation - an experimental ex vivo comparison between piezosurgery and conventional drilling. Clin Oral Implants Res. 2014;25:e140-8.

83. Preti G, Martinasso G, Peirone B, et al. Cytokines and growth factors involved in the osseointegration of oral titanium implants positioned using piezoelectric bone surgery versus a drill technique: a pilot study in minipigs. JPeriodontol. 2007;78:716-22.

84. Stübinger S, Biermeier K, Bächi B, Ferguson SJ, Sader R, von Rechenberg B. Comparison of Er:YAG laser, piezoelectric, and drill osteotomy for dental implant site preparation: a biomechanical and histological analysis in sheep. Lasers Surg Med. 2010;42:652-61.

85. Da Silva Neto UT, Joly JC, Gehrke SA. Clinical analysis of the stability of dental implants after preparation of the site by conventional drilling or piezosurgery. Br J Oral Maxillofac Surg. 2014;52:149–53.

86. Aaron JE. Periosteal Sharpey’s fibers: a novel bone matrix regulatory system? Front Endocrinol. 2012;3:98.