Disclosure to Promote the Right To Information

Whereas the Parliament of India has set out to provide a practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority, and whereas the attached publication of the Bureau of Indian Standards is of particular interest to the public, particularly disadvantaged communities and those engaged in the pursuit of education and knowledge, the attached public safety standard is made available to promote the timely dissemination of this information in an accurate manner to the public.

इंटरनेट मानक

“!ान $ एक न' भारत का +नम-ण”Satyanarayan Gangaram Pitroda

“Invent a New India Using Knowledge”

“प0रा1 को छोड न' 5 तरफ”Jawaharlal Nehru

“Step Out From the Old to the New”

“जान1 का अ+धकार, जी1 का अ+धकार”Mazdoor Kisan Shakti Sangathan

“The Right to Information, The Right to Live”

“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता है”Bhartṛhari—Nītiśatakam

“Knowledge is such a treasure which cannot be stolen”

“Invent a New India Using Knowledge”

है”ह”ह

IS 4723 (1978): Egg Powder [FAD 18: Slaughter House andMeat Industry]

IS ; 4723 • 1918

Indian StandardSPECIFI(~ATI()N r()R EGG P()WI)ER

(F'irst Revision )

\JL) <.~ G') 7 ,1 J

INDIAN STANDARDS INSTITUTIONMANAK lil-tAVAN t 9 HAtiJ\DlJR StlAli Z~FAR MARG

NEW DhLfII 111)002

March \979l

IS .4123 • 1978

Indian StandardSPECIFICATION FOR EGG POWDER

( First Revision)Meat Indusn y Sectional Committee, AI~"DC IB

DR C. M. SINOH

R,prt senl 111g

Indian Vetermary Rcsearr h Iristrtute (ICAR),Izatnagar

Members

DR Ii P ANIlA (Alle,"at, toDI C Mil SIngh)

AORleuT/ruRAI MAI'JrRTrNcl Drrectorate of Markeung & Invpe c tron (MlI1J~try

Al)VIHJ1 R T() THE GO\ lLltNMFN'l of Aglll.ulture & III igauon ), I arrdabadOF INDIA

SI1J~I SII JA v ARAMAN ( Alternate )SHRI D. S. (~HADIIA Central Cornrmtt ee for Food Standar d~ ( Munsu y of

I leaItil & 1 a rml y Wt"If.Jre ), Ne w De1IIIS\f1' 01 lSI MUK1IFIt JJt~~ ( Alternate )

CIIAIRMAN Techrucal Standar drzatton Comrruuee ( Foodvtuffs )( Mimstrv of Agr rculture & Ir i rgat ion }, NewDc-Ihl

SJrCRRTARY ( Altrrnau )DIl S. C .. <"~JtAKltAV()ltTY Metal Box Company of India I.. tel, CalcuttaSaur D. 8. P.. CnANJ)H.AS(4~KHARA Rrglunal Bd-LOIl facto) '), GdUn.iVdld.ln

RAODtt S RAGH AV~N()RA RA() ( Alt~rnllte )

DR A K. CHATTIR __11 Jf Miruvn y of Agrrculnu e & Irrrgauon ( D~partment

of ,\gr)( UllUI e )DJt S. DIVAKAltAN Centr-a] I...cat he-r Research Instuute (C~IR),

MadrasDR K. J. Sc AltlA ( Alternate )

GltN.~TlAL MANAUFJ( Centra l D llry I'ar m, Ahgar hSII RT K. S. BISJI J' ( Alternate )

SnRI ANANll K. G()YLE Essex Farms Private Ltd, New DelhiSHRI AltlJN K. Covr.s ( Alternate )

I-IEAL'I'JI OFFIC.Jf,R Corporatron of MadrasVETERJN Anv OI(IJ'ICER ( Alternat« )

BRIO K. S. KlJTTY Directorate of Rrmount Veterrnary SerVICeI, ArmyHe-adqu.u tr-rv

MAJ S. K. GlTPTA (Alternate)DB R .. R. MALLYAMANAO~R

DR M. N M()ORJANJ

DR B. R. BAI~IOA ( Alternate)

Mateo Factory, BombayRegional Bacon Fac.tor y, Hat ingha tel

Central Food Technological Research Institute( CSIR ), Mysorc

( Contsnued on pa" 2 )

© Copyr.ght 1979INDIAN STANDARDS INSTITUTION

This publication is protected under the 1"dUJn CopyrIght Ac' (XIV of 1957) andreproduction in whole or In part by any means except WI th written perrmssron or thepublisher shall be deemed to be an mfrmgement of copyright under the laid Act.

IS : 4723 • 1978

IU/J"stnlinlMunicipal Corporation of D~lhi

( Contanlltdfrom pag' 1 )Members

MUNICIPAl, HEAI,TB OFI'IC.Fjl\SUPERINTFNDEN'J ( Sr,AUODT­

Eft HOUSJ4; ) ( Altlrnat, )SUSI V. NAGAR AJ u Central Mechanical Engmcering Research Institute

( CSIR ), Durg.ipurDn R. RAM"MtlRTHl Madras Veterinary College, Madras

DR M. P. AH~tTMIJ(.A\1 ( Alt,rnat, )SHRI S. RAl\fA8WAl\'1 \ Accelerated Freeze ])t \ Ing Faetory (~flnlstry of

I)efttnce ), TundlaSHBll".N RAlVAL (All"nat,)

SaRI s. RAPilA8\VAMY Directorate (;en~lal of Technical Developrnent ,New DelhI

Sf:lRI S. N. PANI))l;Y ( Alternat» )Du T. R. SHO\ltlt{A !)t"(t ncr Food R~....arch Laboratorv , Mysore

Suur L A" RA1\I-\No\J "A~ (Alternate )COL R. N. '1' \ Nf4 ,rA Qq,tr It r rnaster Gt'n~lals Branch, Arrnv Head.

quar trrsl. r ..CCl( K. i\ ~C}lA llYA ( Alternate )

SJI nt 'f. Pu H.N A N A " l)A"\I, Jl11 ect or (Jt:ner.ll) I" I ( Ex.oJliclo AIember )Deput} Director ( Agil & l'ood )

S« retary

~ uur !\ f \ N ~ ) II \ n "I " " , 1 " " N JDeput v Dn ector ( Agil & toO(] ), lSI

Poultry Products Subcommittee, AFDC 18: 9Convene,

DR B. PANJlA Indian V..terrnar y Research Insntute (IC:.'\n),Izatnagar

SHut M. A. IIAl.RSM

SIIKI S. T. HAHIRA-MANIBuro KARAN SINUll

M,mb"JSIlK! S. S. VKMMA ( Alternate to

Dr B. PANJ). )AORICULTURAI, l\rIARKKTINf] Directorate of Markermg & In..pernon, MInistry of

AnvISLR TO TilE GOVT OJ' INorA Agricultural & Ir r rgauon, FarrdabadSHn} S. JAYAJI.AMAN ( Alternau }

MA,T V. P. S, Cll()UIJUAlt \"' Quartermaster Gener al'A Branch Army HeadquartersSHItI D. P, GUPTA Drrectorat e of Ammal Husbandry, Governrnent of

R'iJasrhan. jalpurCenn a l Food Technologrca] Research Iustrtute

( (;SIR ), M}sorr-Food & Inns Ltd BombayDirectorate of Remount Veterinary Services, Army

Headquai ters

Dn S .. RANJAN

DR P. BAPA RLDl)Y

MAJ S, K. GUPTA (AlterntUl)DR P. KOTJIANDARAMAN Madra s Vetei mary College, MadrasDR S. K MUKJI}rltJE~ Arbor Acres I arm India Lid, New DelhiSURI p C PANDA Collrf{e of Animal Services, Haryana Agricultural

Umversrtv, HrssarPOUl l 41 r ) Project, ChandrgarhDIrectorate of Animal Husbandry, Government of

Andhra Pradesh, HyderabadMmistrv of Agriculture & Irrigation (Departmellt

of Agriculture)Dn B ApPA RAO ( .4It,rnat, )

2

.,--"'--

AMENDMENT NO.1 JULY 1983TO

15:4723-1978 SPECIFICATION FOR EGG POWDER

(Pil'st Revision)

Al teration----~-

[Page 6, Table 1, col J, against Sl No. (x11)] ­Substitute '50' for '100'.

ReprograPh7 Un!t t lSI, Bev Delhi, India

AMENDMENT NO.2 APRIL 2011TO

IS 4723 : 1978 SPECIFICAnON FOR EGG POWDER

(First Revision)

[Page 5, clause 4.2(c)]- Substitute 'Net quantity of the contents;' for 'Netmass of the contents;'.

[Page 5, clause 4.2(g)]- Substitute the following for the existing:

'g) Any other marking required under the Standards of Weights and Measures(Packaged Commodities) Rules, 1977, and the Prevention of FoodAdulteration Act, 1954 and the Rules framed thereunder.'

(FAD 18)Reprography Unit, 81S, New Delhi. India

IS : 4723 • 1978

Indian StandardSPECIFICATION FOR EGG POWDER

( First Revision)

o. FOR E W 0 R D

0.1 Thrs Iridian Standard ( First ReVISI()}} ) was adopted bv the IndianStandards Insntutron on 16 October 1978, ,:lft( r the draft finahvcd by tileMeat Industry Sectional Comrrut tee had bt ell approved 1>\ theAar icultui al all<1 Food PIoducts Drvisron Councrl.

0.2 The demand for eg~ powder IS inr lC£l\lfl1{ «)rl"l{icl,lbly both Irorn thecrvilran populatiou arid fl()(ll the deft n: C' personnel. M()le()\'CI, thetcchnolou.cal det.uls pr-t tairung t.o its manufacture have- ber-n WOI ked outfully cit the Ci ntr al Food T(~( hnologrc.rl Research Institute, Mysore,I'his standard is llelJ1K formulated 111 (Jl tit'} to ensure t hat the 1)1 oduc tl011

of eg~ powder 1~ 111) to a quahty level t hat IS acceptable ti) the consumersand feasible for th« manuf.u tut ei s

0.3 This stand,n d W~lS first published In 1968. I'he present Tt'\lISIOn wasundertaken mamly with a view to clllgrllllg this standard WIth ASCspecification ft)f rgg powder, Consr qucntly, this standar d mcorpor aressome of tile .uldruonal requu ernents like boric acid, total ash, acidmvoluble ash, organic phosphor us pent.ioxide ( P20~ ) and Salmonella.Besides, the requirement Ior pH lla~ bee 11 deleted a nd the method fordeterrnina tron of solubihtv has been modified

0.4 III the preparation o! this standard, due consider.itron has been givento the pr ovisrons of thr Prevention of Food Adulteration Act, 1954, andthe Rules fl amed thereunder. However, this standard IS subject to therestrrctrons imposed under this ALt wherever applicable.

0.5 III the pre pat at ion of this standard, considerable ..lSS rstance has beenderived from the following publrr .mons:

CFS 2 3-1 B Eggs, dr ied Commonwealth ~'ood Spec ifications,Commonwealth of Australia.

ASe Specification No. 57 Egg pOWllCI (spra}' dried). DIrector ofSupplies .md Transpoi t, Almy Headquarter s, Mmistry ofDefence, Government of Indra.

3

IS : 4723 • 1978

0.6 1 111is standard contains clauses 4.1.2, 4.2 and E-2.3, which call for anagreement between the purchaser and the supplier at the time of placingorders.

0.7 For the purpose of decid ing whether a particular requirement of thisstandard is complied with, tile final value, observed or calculated,expressing the result of a test or analysis, shall be ron ndcd off ill

accordance with IS: 2-196()*. The number of significant places retaine-din the rou ndr .. (1 off value should be the same as that of t11C' specified valuein t }1is ~ t ,1rida I d .

1. SCOPE

1.1 TI\i~ st a n.lard prr-scribes tile 1C(lltll erncnts ~lI1J the 111CtllO({S ofsampling and tcvt for eg~ powder.

2. TERMINOLOGY

2.0 For t hr- llllll)()\(' ()f this st.mrlarcl , tile following definition shall apply.

2.1 Egg Powder - The product prepared under hygienic conditionsfro 111 t he Iiq {lifl rontcnts of sound, wholesonu-, hens' (-ggs by anyrccogn izcd method ()f 5111 a Y lll) illg I

3. REQUIREMENTS

3.1 Hygienic RequirelDenta or Proces8ing Unit - The materialshall be prepared and handled under strict lrygicnic conditions bypersons free frorn contagious and infectious diseases and only in premisesmaintained in a thoroughly clean and hygienic condition and havingadequate and sal~ water supply ( see IS : 2491-1972t ), and duly approvedand Iicensed by the public health author it ies concerned. 1\11 workersshall use clean and washed cloth1tlgC;. Necessary preen ut ions shal I betaken to prevent incidental contaminat ion of the product fiorn soiledequipment or from personnel suffering flOIU injuries.

3.1.1 All equipment corning ill cont,u t with raw materials or productsin the COllfSC of manufacture shall be kept clean. An ample supply ofsteam ancl water, hoses, brushes and other equipment necessary for pr()p(~r

cleaning of machinerv and equipment shall be available. The equipmentmay be sterilized by immersion in or swabbing with hypochlorite solutionor ot'her suitable chlorine solution.- -~---- --------...........-- -

*ltulrs for round ing off nurner ical values ( r,vls,d ).tCode for hygienic condurons for food processing units (fir-lt r,v;ju)1J ).

4

IS • 4723 • 1178

3.2 Processing Requieements3.2.1 The eggs, before break ing, shall IJe properly washed, dried and

cooled, i f necessary.

3.2.2 (; lucose present in the liquid contents of original f'~~~ shall beremoved before dr Ylng-.

3.2.3 Tile Jiquid contents of t 11~ f'g"~~ shall be pasteurized IJy heatingfor 5 minutes (it fil to fi2°C in a pl.ue tYr)(· l)t!..,t( ur izcr 01 by any othersuit.ible method.

3.3 Requleements of the Finished Product3.3.1 'The eg~ powder shal l 11(lVC a uniform yellow or 01 ange-ycllow

colour arid a smooth .i nd llf11ft)1111 textui e, t111(] shall be fJ ce fl01]1 lumpsand gr it ty II1Ll tc I ial.

3.3.2 E~~ powder sh.rll J ct.rin tI1C' original proper ties of fresh egg, hkeSOIL!b ihty of 1)1 otcin, del at iIlg cap.icit ~r, bind ill ~ I)()W(" rand 1).1] atabi 11ty,Eg~ powder sh.rll i cconsntut... 1 c.idi ly and quicklv when It i~ mixed withthi ee times its m.iv- of Iukew.irrn wetter ( about 4(jOC ), after a prclirnmarymixing to form d smooth paste. On reconstitution, the egg powder sha llbe fl ee from unpleasant off-flavours,

3.3.3 Eg-g powder tnay contain a(l(l(~(l carotene and riboflavin.

3.3.4 Eg~ powder shall be flee from di-coloration, added prescr vat ivcs,artificial colouring matter and pathogenic micro-or g(ltli')m~, anti otherextraneous matter.

3.3.5 The product shall also COllI ply with the chemical aI1U micro­biological requirements glVCIl ill Table 1.

4. PACKING AND MARKING

4.1 Packing

4.1.1 Egg powder shall be g-,l~ packed ill nitroacn (01 nitrogen andcarbon dioxide ) In suitable t mplate or flexible container-s.

4.1.2 Packing in Cases - The containers shall be packed in suitablecases. The number of contamer s in Cel<. h case sh.rll he ';1I bjec.t to agree­ment between the purchaser and tII(" packer,

4.2 Marking - The corrtainer S shall he marked either by printing orIithographing 011 tile containers themselves or by attaching labels printedon pc.lper as agreed between the purchaser and the vendor. The markingor the label shall give the following information:

a) Name of' the material along with brand name, if any;b) Name and address of the manufacturer;

5

IS : 4?23 • 1978

TABLE 1 REQUIREMENTS FOR EGG POWDER( Claus« 3.3.5 )

CHARACTERI8'rIC R~:C~lrJRE­

M}4~NT

ME1'}IOI) OF TR81', RE}4" TO

r-------~-------..Appendix of Other

This Standard Standards

1) Moisture content, percentby mass J Max

[i) Protem ( N X 6-68 ). percentby mass, MEn

IJi) I ..ecithin and fat, percent bymass, Min

iv) Solubrlitv , percent by mass,Min

v) orre acid

vi) Organic phosphorous pen­tOXI(!r ( p.06 ) t percent bymass, Min

vii) Total ash, percent by mass,Max

vm) Ash insoluble in hydrochloricacid, J>Cl cent by mass,Mas

ix) OX} gen content, percent byInaS4\, Max

x) Total plate count, per gram,M« ..t

xi) Yeast and mould count, per~ram, Max

xii) Coliform fount, per gram,Max

xiri) Salmonella

(1) (2) (3)

20

40 0

85-0

Absent

o 10

75000

50

100

Absent

(4)

A

Il

D

(5)

IS : 7219-J973~

(~l 23.1 ofIS: 1479( Part II )-1961t

Cl 19 ofIS : 1479( Part I I )- 1961t

Appendix E ofIS : 1547.196Rt

Appendix F' ofI~ : 1547.196Rt

IS : 5402-19G9§

IS : 5403.1969U

IS : 5401·1969~

I~ : 5887( Part 11)­1976··

*Meth.od for determination of protein in food. and feeds.tMt't}lOds of test for dairy industry: Part II Chemical analysis or milk.iSpeclfica tion for infant milk foods (first r,vislon ).§Method for plate count of bacteria in foodstuffs.IIMetllod for y~alt and mould count of foodstuffs."M~lbods (or detection and estimation of coliform bacteria in foodstuffs.··Methods for detection or bacteria responsible for food poisoning: Part III Isolation

and identification of Salmo",ll" and Shi"IID.

6

IS : 4723 • 1978

r ) Net mass of the contents,

(I) Batch number or code number ;

e) Names of the uurredrents,

f) 1..1CC11 CC 1111 In1)C1 g1V C n 1)y t 11 e 11 C (\1 th .iu t II ()r Jt IeS, d n d

g) Other requu ernr-nts ..rs P'" the Weights and Measur- s (Patka~cdCommodities ) Rules, )q77

4.2.1 E ich container nlay also be mar keel Wit 11 the IS I eel t ificationM,llk

NOTE - The use of the I~I Cernfication Mark IS governed by the provisions of theIndian Standards Inst nuuon ( Certrficatron Marks) Act and the Rules and Regulanons"lade thereunder. The 1"1 mark on products cove-red by an Indian Standard conveysthe assurance that they have been produced to comply with the requirements of thatstandard under a well-defined svste-rn of inspecuon, testing and qualny control whichIS devised and superv rved hy lSI dod operated bv the producer I~I marked productsare also conunuouslv checked by I~I for conformuy to that standard a, a further safe­guard Detarls of rondmons under wluch a hcence for the use of the I~I CerrrficanonMark may be granted to manufacturers or processors, may be obtamed from theIndian Standards Institution

5. SAMPLING

5.1 Tile mct horl of di awrnq lel)l esrntat rvc <;'lnlJ»( s of the mate rr.rl ,111(1

the cr iterr.i f()l conforrrut y ,11l11 })("-. cl~ 1>1 t·~( Il1)p(1 111 i\P~lCIIlIJX I~.

6. TESTS

6.1 Test s sh.i ll l)c cal] led out .is III {'~( t ibcd In the (l ppro prr ..ite appendicesgIven under c ol t ()f Ttll)le 1

6.2 Quality of Reagents - Unless -pccrfied otherwise, I)LJI{~ chernicalssual! be employed m tests and distrlled water ( see IS : l070-1~)77* ) shallbe used where tile use of water .is ,1 I r,lgeIlt 1~ mtcndr d.

NOTE - 'l)ure cherrncals " shall mean c hermcalx that 00 not contain rrnpurineswhich affect the results of analysis

6.3 Preparation of SalDple for Microbiological Tests - W(~lgI1 11 ~

of the materral from tile individu \1 sample usmtr a stcr ile spatula andsuspend III 99 ml of di luuon water at 45.JC. Agltat~ rmldly, soak for 1to 3 minutes and then agrtate vigorously to avoid chur nmg out tile fat.Prepare drlut ions of this and add 1 rnl of suitable dilutions In triplicateto the stcr ile petri drshes.

*Speclflcatlon for water for general laboratory use ( second rnnsion )

7

IS : 4723 • 1978

APPENDIX A[ Table 1, Item (i) ]

DETERMINATION OF MOISTURE CONTENT

A-I. APPARATUS

A-1.1 Flat-DottolD Dishes - of glass ()I aluminium and with covet.Dishe- should not he affected by boihng water. They 111ay bt a 7 t() 8 ernill drametrr and not more than 2·5 ern dC~I). '1'11CY should })C providedwith short glass st irring rods having a widening fiat end.

A-l.2 Well-Ventilated Oven - ma int.rined at 100 ± 2°C.

A-2. PROCEDURE

A-2.1 Wcigll accur.itcly about 5 g of thr- sample, into ,1 Ilat-bottom gt,lsSor alumimum divh ( with a cover ) previously dried and weighed. Heatthe dish r.ont.rining th e mater ial after uncoverrng III all oven maintainedat 100 ± 2°C for about 5 h()UIS. Cool ill 1;1 desiccator and weigh wrththe cover rm, Repeat tile process of drying, cooiinj; and welgl1illg athal f hourlv inti rvals, until tile difference betwr-en t We) convecut ive wi Jg}l­

111gS is less than 2 r11~. Record tile lowest 111(155.

A·3. CALCULATION

A-3.t Moist ur e content, percent by mass = }OO ( A-lt -_M2_)( All - AI)

where

iV/t ::c IJlaSS ill g of sample with the dish,

M 2 = mass in g of dried sample with the dish, and

M = mass in g of em pty dish,

APPENDIX B[ Table 1, Item (iii) ]

DETERMINATION OF LECITHIN AND FAT

8·1. APPARATUS

B-l.1 Soxhlet Extractor

B-2. REAGENT

8-2,1 Chlorofornt

8

IS : 4723 • 1978

B-3. PROCEDURE

B-3.1 Weigh accurately about 2 ~ of the sample into the folds of (1 filterpaper ( WllatrllaIl No.4 or equiv.il.-nt ) el11(1 extract with chloroform for16 hours in a. Soxhlr-t extractor. })lSC()11I1CCt the t ar e<l Soxhlet Ilask ,distil off thr- chloroform completely and weigh the ll.rsk with the residue( Jer i thin and felt ).

8·4. CALCULATION

B-4.1 Lecithin and fat, percent by mass = 100 ( A12 - At! )~ ----Af

l

where

Af2 = m.iss ill ~ of the residue wrt h Soxhlct Il isk

M = mass ill ~ of ernptv Soxh let fl.isk , ;111<1

M1 =--= mass in ~ ()f sa 1111)lc.

APPENDIX C[Table 1, Item (iv)]

DETERMINATION OF SOLUBILITY

C-l. PROCEDURE

C-l.1 Weigll accurately 1-0 -t O'] g of the s.unple ill an or dinar y testtube and .idd cx.ict lv 5 ml of 5 percent ('lIfo) sodiurn chloi ide. Closethe tube wit h a lubber stopper and shake gcrltly fUJ I 111illUtC t o dispenset IlC' powder. Set aside for 1J 111 inut CS ,t 11(1 i 11 vel t t.: 11 ti Illes. Aftr-r5 minutes, (lose with tile index linger, tile t()P ()f tlu- convenient lengthof g-la~~ tubing ((\})})1()X. 2 111111 bor c ) and iuvci t the tubing under tile'

top of the hquid rotated tll()rC)tlglll)'. ()J)(-Tl tile tC)P of tile tubemomentarily, close again and remove the tube from solution .md wipethe outside of the tube. 'Transfer a rlrop ()f t he liquid t o t he I rfi acto­meter and read off the J efract ive i ndex,

C-l.2 Determine the solubility index ()f the ~g~ powder by rt~fi actoincter( Haenni value ) as f<J]]()WS:

Haenni value ~ ( A· - r) X 1 000

where

X ~ refractive index of tile sample solution, and

r = refractive index of the solvent.

9

18 I 4723 • 1978

Calculate the solubility percentage from the Haenrn value as follows:

L()glo ,y = 0-445 + 0 01 x

\\ llel t'

y = Haenm value, and

x = pr-rci ntagc solubihty In sodium chloi Ide.

e-I.3 For the s ike of C011venlCI1C<", the following table mel Y be rcfcI Jed tof01 (011vel sion ol Haen TlI val uc to the solubrhty l)er(,·f'Ilta~(

Haennt Value Solubllzty Percentage

17 78 55I ~~ 81 03

19 83-36zo ~15 (JO

21 87 72

~2 900023 91-67

24 93 5225 95-292{) 97 00

27 QU 64

28 1(10 00

APPENDIX D[ Table 1, Item (ix) ]

DETERMINATION OF OXYGEN CONTENTOF CONTAINERS

D·}. APPARATUS

D-l.1 A diagrammatic sketch of the recommended apparatus, asassembled, IS shown In FIg_ 1.

D·2. REAGENT

D-2.1 Pyrogallol or 1,2,4-TriacetosybeazeDe - Either of these twoabsorption reagents may be used.

10

f'IG

.1

DIA

GR

AM

MA

TIC

SK

ET

CH

OF

Oxvczx

AN

AL

,TS

ISA

pP

AR

AT

L·S

-... fI) .. .......~ w • .....

.\C ~ CD

H

G

MIR

RO

RS

CA

LE

F

IND

EXM

AR

K

c

A

SA

MP

lNG

LIN

EN

TO~

,V

AC

UU

MP

UM

P

....

IS I 4723 • 1978

D-2.1.1 Alkaline pyrogallol is prepared by mixing equal volumes ofsolutions of25 g of' pyrogallol in water to 100 InI and 100 g of potassiumhydroxide in water to 100 ml, and kept in a well-stoppered bottle,

D·2.1.2 Alkal ine I ,2,4.triacetc)xybenzene is prepared 1))' d issolving109 of 1,2,4-tri"lcet()xyl)enzeJlc by gentle warming with ,1 solution of13-5 g of potassium hydroxide in 100 m l of water,

D-3. PROCEDURE

D-3.1 With the sampling tool resting on IJtJt not l)jercing the can orcontainr r, and with stopcock B closed and stopcock t>J\'. ()perl, evaquatetile sampling line to 5 mrnHg or less, as indicated on the Ill,1110111etl'l;

close stopcock N, and if there is 110 leak in tIle s.unpl ing line tile mano­rueter level docs not change, (If the level changes, indicating It leak,this is rectified and the Iine is rc-eVdCllatell.) Wit}1 N closed, pierce thecan or container when the mercury ill the manometer [lows back with aclick. Keeping stopcock G open and reservoir H on the lowest hook,turn stopcock B to allow gas Irom tile sampling line a nd can or containerto Ilow into C until one or both bulbs are full of gLlS. Close stopcock 0,stir the water in the jacket and note its terupc-rat ure ( TOC). Bring thelevel of mercury in C to the appropriate index mark by closing (; andoperating the screw elil). Thrn take the manornt fer rcadi ng ("S'l)_ Hangreservoir H on tile t()P1TIC)st hook, open stopcock G and turn stopcock Bto pass the ga,; into the absorption chamber, By raising and lowcrirlf{ H,the gas is passed to and fro tile requisite number (If times, Whenabsorption is complete bring the reagent level to the mark, approximatelyby moving reservoir H., then exactly by closing (; and operating the SCIe\Vclip. Close stopcock B, open stopcock O, arid adjust tile mercury levelin the measuring bull) to the appropriate mar k ,18 before, Note themanometer reading (S2) , and read the thermometer. (Tllrfc shall beno tcrnperatut e Ch(111ge during the estimation; any change will causeserious errors if not allowed for in the ca.lcul;lti()n.) Finally discharge,the g'clS remaining ill the measuring lJU Ills G' by raising }f, opening (;, andt urning B t() allow the gas to Ilow out through the sampling line, follow­ing up with mercury until the llpper bore of B is full.

D-4. CALCULATIONL)l - S9

D..4.1 Oxygen, percent by mass = B _ ltV _-P+ Sl X 100

where

81 = manometer reading with sample at index mark beforeabsorption,

S2 = manometer reading with sample at index mark afterabsorption,

12

IS I 4723· 1978

B = barornetrrc pI essurc,

~'V = ,\CttJeOtl\ Vap()Ul IJ1CSSUrc at water jackc t temperature( TOC ), .md

P = manometer 1eadmg C()) f( sporidmg to index mark at.urnosphc t 1C P' essu I C ( PI ()I P2 ,l' ~ IJ!lr<J!)11dte ).

Nor.. - If the oXY~r'n content 1~ low (\lndcl ') percenr ) , the barometr icpre-sur e Ina v be avsurned to be 76 0 cn1Ifg and W to be 1 5 (<..orrc'\pondlngto 18°t"). If In addition \1 It; about tile same as P + 0 2 crn l lg, the denorm­nator IlhlY he assumed to l,f 75 0 m .rll cases without dffc-ctlng the accuracy oft }1('" r~ , u1t tot h t" ill'" t de( I I11..t 1 P11(.r

I lIt n tilt" rXpl ewron br-C0J11C\

\ ( '-,, - S'2 ) "(>", gf"n, percent l)\ mavs - ~ )I 100:= 4- 3 ( Sl - S. )I J

wluch gl\e~ suffi. lent accurucx for {.!(,lOl y contrcl purposes

APPENDIX E( Clause 5.1 )

SAMPLING OF EGG POWDER

£-1. GENERAL REQ,UIREMENTS

£-1.0 In lil awing, P' eparmg, stor inz and handling samples, the followmgprecaution" and d n cctions sh ill he observed

E-l.1 Samples shall be t.ike n 111 a pI erected place not exposed to dampLl1r , d ust o I S( JC)t .

E-l.2 TI1( s.unplmg IIIStl urn. nt shall be r le.m and dr Y when used.WI1( I} t.ikins; samples for bactcr iologrcal ex.unmation It shall be stet ile.

E-l.3 Prcr aut ion- shall IJC taken to pr otc: t the s.unpk s, the materialbeing sampled, the samphng mvt I unu nt , and the cont.uner S for sampleI 1o111 d dvell trt lOll S (0I it Lt. nu nat J(J 11

E.1... The samples shall he pl'lLcd 111 dl'.lI1 <.U1d dry glass containers I'hesample contarncrs shall be of such a siz«, that they ar e .ilmovt comple teJyfilled by tilt' sarnple. Thc sample contrunci , shall, III .idditron , bestci rle when tlu-y .u « used Ioi s.unples fur b.rctcr rologrcal ex.urnn.uron,

E-l.5 Each corrta mcr shall be 5.(' dt d all tl~ht after fillmg .md markedwith full cit tails of s.unplmg, bat: 11 01 code number, 11(lI!}e of themanutactur ci ,J,11(1 other nnpoi t.mt 1)(11 t iculars of tile ("(Jn~lgIIIT1(nt.

lJ

IS I 4723 • 1978

£-1.6 Samples shall be stored in such a manner that the temperature ofthe material does not vary unduly from the normal temperature.

E-2. SCALE OF SAMPLING

E-2.1 Lot - All the containers in a single consignment of one type ofmaterial drawn from a single b.uch of manufacture shall constitute a lot.If the consignment is declared to consist of different batches of manu­facture, the batches shall be marked separately arid the gr()up ofcontainers ill each batch shall constitute separate lots.

E-2.1.1 Sample shall be tested from each Jot for ascertaining itsconformity to tile requirements of t his standard.

E-2.2 Thr- number of containers to be selected fl (JIll the lot shall dependon the size of the lot :111(1 ~11al1 be as givell in Table 2.

E-2.3 The containers shall be chosen at random from the lot ;111CI forthis ptlrI)(lSe a random number t a hle (se~ IS: 4905·1968* ) as agreedbetween the purchaser and the supplier sh.il l 1)(-- used. If such table isnot available, tile following procedure s11t111 be adopted,

Starting from any container, in the lot, count them as 1, 2, 3 .•. ,Ill' to r in a systematic manner, whet e r is equal to the integral partof N]n, N being the total number of conta ine rs in the lot, and n tilenumber of containers to be chosen i se« Table 2). Ever y rthcontainer thus counted shall be separated unt il the requisite numberof containers is obtained from the lot to give' samples for test.

TABLE 2 NUMBER OF CONTAINERS TO BE SELECTEDFOR SAMPLING

( Clauses E-2.2, E-2 3 and E-3.3 )

LOT SIZE

(J )

2 '0 2)

26 " 100

101 ,. 300

301 " 500

501 and above

SA)IPl,E SIZE ( FOR TESTS

OTHER ['HAN MICRO­

BJOr"OOICAL )

(2)

2

3

5

7

9

SlJn-SAMPI..E SIZlC ( 't)R

MIOROBIOl.OOICALTJi,8T8 )

(3)

1

1

2

3

4

• Methods for random sampling.

14

IS 2 4723 • 1978

E-3. TEST SAMPLES AND REFEREE SAMPLESE-3.1 Preparation or Individual SalDple - Draw with a suitablesampling mstr umcnt approximately equal quantrnes of the materral fr orndifferent pal ts of tile C()11tUl11er t ill t lu. quantity collected is about 500 gand divide It mto t ln ce equal parts Each part so obtained shallconstitute ("1 vam ple repr csent ing tll(" Cl)llt,11IlCI and shall be transferredimmedratcly tel thor ouvhlv c le.m £\0(1 dr Y container S Sf alvd all tight \\ ithparticular S f,{IVC'Il under E-I.5 TIle 111(i ividual sample so obtamed shallbe divided into three set" III such a \vay that c..ich -,et has a sample,reprcsentiug each velec ted c ontainer. One of these shall be marked furthe purchaser t another for the vendor arid the third it)} t he referee

E-3.2 Preparation of COlDpotlite Sample - From tile material fromeach s..lcc tell <. ont.une i , fC m.uumg 1ftt t t he mdrvidua l sample has beentaken, approxim.itr-Iv equal qu.mt n r. c; ()f t hc mater ial shall IJ~ taken andIn ixed t ()~ t her so ,1S to Io: m (.\ ( ('rlll)O~ It(· varnple weighing a bou t 600 g.~1'111\ LOfTl}> )~lte \dITII,le ~11,111 be d ivided IIIll) three equal parts andtt a nsf rr (~ll t o clean and (il y c.ont a mer v ~lt.llc(l au ught and labrlled wuhrh- part u ul.u s ~l\ r-n III E-l.5. One ()t t ht sc composite samples sha ll bet~)1 the ptlt ch.is. r , .mot hci f()) the vC'I1<1()} .md the t hrrd for the referee.

£-3.3 Preparation of Samples for Microbiological ExalDiDatioD­From t he sel. ( tr J r ont.uue r ~(lt-( t (1 ~l11)-S(1.Ill1)lt accor drng to col 3 ()f

T &:1b1e 2. D raw \VI t 11 4\ cit11t 411)Ip ~ t III I)II t 1g' 111e, ( 1 U IncT1t, "\ IIICIt 1sstC J 11 e , (it

least 100 g ()t the m ..itei i.i l and nux thor «uvhtv u nr lcr d~eJ)tl( C(.)11dJtlOI1S

to form t-l sample t u Tll1lr()1)}()ll)~1( c:11 t XilI11111,1l)()n. Divrdc the -.unple( takmg (ell CO 11()t t() bi mu III rruc. obiolou«. II contaminatron In tilt'm.itr-rral ) into t ht ~C' cqu.il p.u t~ }~ u h pelt t so obt.uned shal l constitutea sample rr pI (~~(1I11tlIl~ t hr }).:\I t-, ~:a( 1\ l),ll t vo obtarned shall constitutec\ ~ 1. m pl(. r t·ll [CSr n t l Il1{ t h e t f) nt ,1111e t d n (1 ~ 11 a 11 be t ran ~ fer I ("d t () s t e r11eglfl:'~ (t)lltdlllel" Sl dit <I, alI11~}lt ,lll(l l&~ l>ellcd Wlt}l P,tt llLllldl S glV~)l

1n E-l.5. 1<tl1f'Y "tllll t)(.. 1114>\1 kc~(t, 1[1 ~l(illlt1()ll, Witli tile WOI (l~ 'ForM1CI Obl()loglca 1)J,,~\nl1natioTl' ~rlll~ SlllI1lJl<. S ,,() (}})tcllIll d shall br ()}\'ldftcllrlto thIef- "ets In 4\tj( 11 d wa\' tlldt Cell 11 ,( t Il&\~ a S{lnlIJ!(- ICI>rescntlng eachsel~ct~(l C(lnt,llllt~t ()Il~ of tll(--sC St~t~ ~11,\11 be lnclrked f()f tIle l)llrcllas~r,

~lnotll~r lc)r tIll· V( rl(lol cllld till. tllild f()1 tIle lefelCIlCl.

E-3.4 Referee SaDlples - R( fel ee g(\rnplcf.i ~hall C()[l~l~t of a set ofIlldlVi(JUcll sa.mI)l~'\ ( E-3.1 ) dlld .:t c()Inl)o~lte ~tlII1I)lc (E-3.2 ) dIld set of~amplts for mlct obl()I()~lc,ll f"Xd.muMtlon ( E-3.3 ) mcil ked for this purposean(l shall l)C,lI th~ ~( (ll~ ()f tIle I>UI C}lclS( I and the vendol. The~l' sl1311 bekept at a pL.lrt· .l'i .1~1 ct"d b<.'twcen the two.

E-f. NUMBER OF TESTSE-4.1 rests tor rleSC11I)tl()n, In()lSlurc, pi otein, lecltlll11 and f~t, boricacid, orgamc P20" ash Ulsoluble 1Jl hydrochlonc aCId, total ash andsolublhty shdll be cOIl<lucted on Lach of the Samples con'ltltutlllg.1 set ofilldlvldual sdll111lcs.

15

IS ,4723 -1978

E-f.2 Tests for bacterial count, yeast and mould count, coliform countand Salmonella shall be conducted on eacll of the samples meant for'microbiological examination',

E-4.3 Test fur oxygen content shall be conducted on the compositesample.

&-5. CRITERIA FOR CONFORMITY

E-S.l The lot shall be declared as conforming to all the requirements ofthis sprcification when E-S.1.1 to E-5.1.3 arc satisfied.

E-5.1.1 The test results on each of the individual s.unples fordescription, moisture, protein, lecithin and f,lt, boric acid, organic P20 .\,ash insoluble in hydrochloric acid total ash, anti solubility shall satisfy tilecorresp()ncliIlg requirement given in 3.3.1 to 3.3.4 and in Table 1.

E-5.1.2 TIle test results for bacterial count, yeast and mould count,coliform count and Salmonella shall satisfy the corresponding requirementas given in Table 1.

E-5.1.3 The test results ()O the composite sample for the characteristicmentioned in E-4.3 shall satisfy the cor: esponding requii cment asspecified in T able 1.

16

INTERNATIONAL SYSTEM OF UNITS ( 81 UNITS)

Ba-sf' Unit..

Q_r"" A "',T {TY

J.ength

M ..l')S

l"lnlr

EIC""ctr,( currr-n t

I'hr-r mod ytld file

t tt m f>r- r d ( U t r

Lunnnous in \~n~it \

Amount of 'Juh~tao~t'

Uxrt SVMDt)L

n)... tr~ m

" .1 oKram kgfI(""('ond •a rnp-re t\

kelvm K

«lode-I" cd

(UOI(" mul

Suppl~mentaryUnite

QTJ AN'I l'f)

Plane- angt~

Solid anR!~

fddlan

steradian '1Df"rivpd Units

Force

Energv

Power

.~lu~

r"'lux drt15Jf V

Frequenc yElecrrrc conductance

Pressure, Sll ~s!'

Ul'1rr SVI\1{i(}r.. (~()N 'IE H~'()'"

n~w(f)n N N .. 0 101 972 kgfIoutr J J--tNrn

watt vv I W ., r J!~

w,.h~r Wb 1 WlJ .~ I V 5

t C-~ la 1 J 1 .:JlI I Wo/m l

h..-rt, t-J7 1 H7 .a I c/ft (.-1)

siemena S • S ~ t A/V

pascal Pd I l)a -=- 1 Njfn'

-------- ......................... -,

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