ionpac as11 and as11-hc anion-exchange columns · using sodium or potassium hydroxide gradient...

The IonPac AS11 anion-exchangecolumn provides fast profiling ofinorganic anions and organic acidanions using sodium hydroxide orpotassium hydroxide gradient elution.The IonPac AS11-HC is a high-capacity anion-exchange column withselectivity similar to the AS11 column.The AS11-HC is designed to resolve alarge number of organic acids andinorganic anions in complex matricesusing sodium or potassium hydroxidegradient elution. The AS11-HC is idealfor the determination of trace compo-nents and for profiling organic acids inuncharacterized samples.

IonPac AS11 FeaturesUsing a hydroxide gradient of 0.2

to 38 mM sodium hydroxide, the AS11column separates a large number ofinorganic anions and organic acids inapproximately 15 min, as illustrated inFigures 1A and 1B.

Peak broadening and overloadingcan occur when higher concentrationsof analytes are injected onto the AS11column, as shown in Figure 1A. Theseproblems can be eliminated by dilutingthe sample before injection, as shownin Figure 1B. Conversely, the high-capacity AS11-HC column allows moreconcentrated samples to be directlyinjected without overloading thecolumn or affecting peak shape, asillustrated in Figure 1C.

The AS11 also provides rapidelution of strongly retained ions such asiodide, thiocyanate, and thiosulfate incomplex matrices.

IonPac AS11-HC FeaturesThe high-capacity AS11-HC

column allows the injection of moreconcentrated samples without overload-ing and peak broadening. The AS11-HCprovides improved separation over theAS11 column for monovalent carboxylicacids, including quinate, lactate, acetate,propionate, formate, and butyrate. Awide range of organic acids and inor-ganic anions can be separated inapproximately 40 min using a sodiumhydroxide gradient at a controlledtemperature of 30 ˚C, as illustrated inFigure 1C.

colum

ns I o n P a c ® A S 11 a n d A S 11-H CA n i o n-E x c h a n g e C o l u m n s

Key ApplicationsThe AS11 and AS11-HC columns

are solvent-compatible, which allowsfor anion-exchange selectivity controland easy column cleanup after theanalysis of complex matrices. Typicalapplications include the determinationof organic acids and inorganic anions indiverse sample matrices including:• Fruit juices and wines

• Foods and beverages

• Fermentation and process solutions

• Chemical additives

• Chemical process solutions

• Wastewater

• Brines

• Power plant waters

Economical Microbore OperationThe IonPac AS11 and AS11-HC

columns are available in the 2-mmformat for microbore operation to offerthe advantage of reduced operatingcosts.• Ideal for limited sample volumes

due to higher mass sensitivity.

• Three- to fourfold reduction ineluent consumption.

• 4-mm applications can be directlytransferred to the 2-mm format byreducing flow rate by fourfold.

13485

Figure 1. Comparison of the AS11 and AS11-HC columns for the separation of a wide rangeof organic acids and inorganic anions using sodium hydroxide gradient elution. The high-capacity AS11-HC provides excellent peak shape for early-eluting anions.

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Columns A & B: IonPac AG11, AS11, 4 mm

Eluent: Sodium hydroxide:0.2 mM from 0 to 6 min,0.2 mM to 5 mM from 6 to 11 min,5 mM to 38 mM from 11 to 23 min

Flow Rate: 2.0 mL/min

Temperature: 30 ˚C

Inj. Volume: 10 µL

Detection: Suppressed conductivity,ASRS®-ULTRA 4 mm, AutoSuppression®

recycle mode

Column C: IonPac AG11-HC, AS11-HC, 4 mm

Eluent: Sodium hydroxide: 1 mM from0 to 8 min, 1 mM to 30 mM from8 to 28 min, 30 mM to 60 mM from28 to 38 min


Temperature: 30 °C

Inj. Volume: 10 µL

Detection: Suppressed conductivity,ASRS-ULTRA 4 mm, AutoSuppressionrecycle mode

Peaks: B A & C1. Quinate 2.5 10 mg/L (ppm)2. Fluoride 0.75 33. Lactate 2.5 104. Acetate 2.5 105. Propionate 2.5 106. Formate 2.5 107. Butyrate 2.5 108. Methylsulfonate 2.5 109. Pyruvate 2.5 10

10. Chlorite 2.5 1011. Valerate 2.5 1012. Monochloro-

acetate 2.5 1013. Bromate 2.5 1014. Chloride 1.25 515. Nitrite 2.5 1016. Trifluoroacetate 2.5 1017. Bromide 2.5 1018. Nitrate 2.5 1019. Carbonate 5 2020. Malonate 3.75 1521. Maleate 3.75 1522. Sulfate 3.75 1523. Oxalate 3.75 1524. Ketomalonate 5 2025. Tungstate 5 2026. Phosphate 5 2027. Phthalate 5 2028. Citrate 5 2029. Chromate 5 2030. cis-Aconitate 5 2031. trans-Aconitate 5 20

High-Efficiency Particle StructureThe IonPac AS11 and AS11-HC

column packings have unique structurescomposed of a highly crosslinked coreand a MicroBead® anion-exchange layerattached to the surface, as shown inFigures 2 and 3. The substrate for theIonPac AS11 is a 13-µm diametermicroporous resin bead. The AS11-HCcolumn substrate is a 9-µm diametermacroporous resin bead. Both columnsare composed of ethylvinylbenzenecrosslinked with 55% divinylbenzene.

The anion-exchange layer isfunctionalized with quaternary ammon-ium groups. This anion-exchange layerhas a controlled thickness, whichresults in excellent mass-transfercharacteristics and, consequently, veryhigh efficiency peaks.

Solvent-Compatible PackingBecause the IonPac AS11 and

AS11-HC columns are 100% HPLCsolvent compatible, organic solventscan be used for efficient columncleanup or to enhance sample solubil-ity. Time and expense can be savedthrough the elimination of time-consuming sample preparation steps.This feature allows complex matricesto be analyzed with minimal samplepreparation and extends the utility ofthe column to new applicationsrequiring solvents. Adding organicsolvents to the eluent modifies columnselectivity and enables the elution ofnonpolar analytes or contaminantsfrom the column.

8674

Figure 2. Structure of an IonPac AS11 packing particle.

13276

Figure 3. Structure of an IonPac AS11-HC packing particle.

6.5 µm

Anion-Exchange Latex CoatingHighly Crosslinked Core

85-nm DiameterMicroBeads

Ethylvinylbenzene-Divinylbenzene Core

Anion-ExchangeLatex Coating

Highly Crosslinked Core

70-nm DiameterMicroBeads

Ethylvinylbenzene-Divinylbenzene Core

IonPac AS11 Column for Fast GradientProfile of Organic Acid Anions

The IonPac AS11 columnprovides fast profiling of organic acidsand inorganic anions using sodium orpotassium hydroxide gradient elution.The AS11 column is ideal for thedetermination of organic acids andinorganic anions at similar concentra-tions or organic acids in well-character-ized samples. Mono-, di-, and trivalentorganic acids as well as inorganicanions can be separated in approxi-mately 15 min using a sodium orpotassium hydroxide gradient.

Determination of Short-ChainOrganic Acids

Weakly retained organic acidsand inorganic anions commonlyencountered in the food, beverage,biopharmaceutical, chemical, andpower industries can be determined in asingle run using the AS11-HC column.Resolution of monovalent organic acidsand improved peak shape are twobenefits of the high capacity of theAS11-HC.

Increased Flexibility for MethodsDevelopment

The solvent compatibility of theIonPac AS11 and AS11-HC columnspermits the use of HPLC organicsolvents in the eluent to modify ion-exchange selectivity. Both hydroxideconcentration gradients and organicsolvent gradients combined withelevated temperature can be used toachieve optimum resolution of closelyeluting analyte pairs. The retention ofthe more hydrophobic member of theunresolved pair is decreased more bythe addition of solvent, which improvesresolution. Figure 4 illustrates the effectof solvent on selectivity of monovalentand divalent organic acids on the AS11and AS11-HC columns. The AS11 andAS11-HC columns can be operated atambient or elevated temperatures. Theuse of a controlled column temperatureensures reproducible results.

IonPac AS11-HC for Monovalent andDivalent Organic Anions

The AS11-HC column providesimproved separation of monovalent anddivalent organic acids and inorganicanions using a sodium or potassiumhydroxide gradient at a controlledtemperature of 30 ºC. Because highereluent concentrations can be used toseparate monovalent anions, the highercapacity allows injection of moreconcentrated samples without overload-ing and provides improved peak shapesfor monovalent organic acids in com-plex matrices, as illustrated in Figure 1. 13486

Figure 4. Effect of solvent on AS11 and AS11-HC selectivity.

Column A: IonPac AG11, AS11, 4 mm

Eluent: Sodium hydroxide:0.2 mM from 0 to 6 min,0.2 mM to 5 mM from 6 to 11 min,5 mM to 38 mM from 11 to 23 min;Methanol: 10% from 0 to 6 min,10% to 16% from 6 to 11 min,16% from 11 to 17 min,16% to 10% from 17 to 23 min


Temperature: 30 ˚C

Inj. Volume: 10 µL

Detection: Suppressed conductivity, ASRS-ULTRA 4 mm,AutoSuppression external water mode

Column B: IonPac AG11-HC, AS11-HC, 4 mm

Eluent: Sodium hydroxide:1 mM from 0 to 8 min,1 mM to 30 mM from 8 to 28 min,30 mM to 60 mM from 28 to 38 min;Methanol: 10% from 0 to 8 min,10% to 20% from 8 to 18 min,20% from 18 to 28 min,20% to 10% from 28 to 38 min


Temperature: 30 ˚C

Inj. Volume: 10 µL

Detection: Suppressed conductivity, ASRS-ULTRA4 mm, AutoSuppression external water mode

Peaks: A B1. Quinate 2.5 10 mg/L (ppm)2. Fluoride 0.75 33. Lactate 2.5 104. Acetate 2.5 105. Glycolate 2.5 106. Propionate 2.5 107. Formate 2.5 108. Butyrate 2.5 109. Pyruvate 2.5 10

10. Valerate 2.5 1011. Monochloro-

acetate 2.5 1012. Galacturonate 2.5 1013. Bromate 2.5 1014. Chloride 1.25 515. Nitrite 2.5 1016. Sorbate 2.5 1017. Trifluoroacetate 2.5 1018. Bromide 2.5 1019. Nitrate 2.5 1020. Glutarate 2.5 1021. Succinate 3.75 1522. Malate 3.75 1523. Malonate 3.75 1524. Carbonate 5 2025. Tartrate 3.75 1526. Maleate 3.75 1527. Fumarate 3.75 1528. Sulfate 3.75 1529. Oxalate 3.75 1530. Ketomalonate 5.0 2031. Tungstate 5.0 2032. Phosphate 5.0 2033. Citrate 5.0 2034. Isocitrate 5.0 2035. cis-Aconitate 5.0 2036. trans-Aconitate 5.0 20

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13487

Figure 5. Separation of inorganic anions and organic acids using a large-loop injectionon the microbore (2 mm) AS11-HC column.

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Temperature: 30 ˚C

Inj. Volume: 1.0 mL

Detection: Suppressed conductivity,ASRS-ULTRA 2 mm, AutoSuppressionexternal water mode

Peaks: 1. Fluoride 1.0 µg/L (ppb)2. Acetate 3.03. Formate 1.04. Chloride 1.05. Nitrite 1.06. Bromide 2.07. Nitrate 2.08. Carbonate –9. Sulfate 1.0

10. Oxalate 1.011. Phosphate 3.0

13488

Figure 6. Comparison of the AS11 and AS11-HC columns for the analysis of beer spiked with2 mg/L (ppm) butyrate.

Column A: IonPac AG11, AS11, 4 mm

Eluent: Sodium hydroxide: 0.2 mM from 0 to 6 min,0.2 mM to 5 mM from 6 to 11 min,5 mM to 38 mM from 11 to 23 min;Methanol: 16% from 8.5 to 23 min


Temperature: 30 ˚C

Sample Prep: Diluted 1:5, treated with OnGuard™-RP

Inj. Volume: 10 µL


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Column B: IonPac AG11-HC, AS11-HC, 4 mm

Eluent: Sodium hydroxide:1 mM from 0 to 8 min,1 mM to 15 mM from 8 to 18 min,15 mM to 30 mM from 18 to 28 min,30 mM to 60 mM from 28 to 38 min;Methanol: 18% from 14 to 38 min


Temperature: 30 ˚C

Sample Prep: Diluted 1:5, treated with OnGuard-RP

Inj. Volume: 10 µL


Peaks: 1. Lactate2. Acetate3. Formate4. Butyrate (2 ppm spike)5. Pyruvate6. Chloride7. Bromide8. Nitrate9. Succinate

10. Malate11. Carbonate12. Fumarate13. Sulfate14. Oxalate15. Phosphate16. Citrate17. Isocitrate18. cis-Aconitate19. trans-Aconitate

IonPac AS11-HC for Trace Inorganicand Organic Acid Anions Using aLarge-Loop Injection

The high-capacity AS11-HCallows large injections of high ionicstrength samples without loss of peakefficiencies and eliminates the need forsample dilution. High capacity allowsfor the determination of trace inorganicanions and organic acids using a large-loop injection. Figure 5 illustrates theseparation of inorganic anions andorganic acids using a large-loopinjection with a sodium hydroxidegradient at a controlled temperature of30 ºC. Low ppb levels of these analytescan easily be determined using a1.0 mL injection loop on a 2-mmmicrobore system.

Determination of Carboxylic Acids inComplex Matrices

The AS11-HC column can be usedto monitor the organic acid and inorganicanion content in the quality control offoods, wines, and beverages. The highcapacity of the AS11-HC makes it anideal column for the analysis of organicacids and inorganic anions in complexmatrices. Samples high in ionic strengthcan be directly injected onto the AS11-HC and do not cause overloadingproblems or poor peak shapes formonovalent organic acids (e.g., lactate,acetate, propionate, formate, andbutyrate). Figure 6 compares the AS11and the AS11-HC columns for theanalysis of beer. The AS11-HC columnshows improved peak shapes for themonovalent organic acids. Butyrate is animportant indicator of deterioration offoods and beverages and can be easilyseparated from formate on the AS11-HCcolumn with a sodium hydroxidegradient/methanol gradient step change.

Column: IonPac AG11-HC, AS11-HC, 2 mm

Eluent: Sodium hydroxide: 1 mM from 0 to 6 min,1 mM to 5 mM from 6 to 7 min,5 mM to 12 mM from 7 to 11 min,12 mM to 14 mM from 11 to 16 min,14 mM to 23 mM from 16 to 20 min,23 mM to 45 mM from 20 to 25 min


13489

Figure 7. Analysis of red wine using the AS11-HC column.

13490

Figure 8. Analysis of apple juice using the AS11-HC column.


Eluent: Sodium hydroxide:1 mM from 0 to 8 min,1 mM to 15 mM from 8 to 18 min,15 mM to 30 mM from 18 to 28 min,30 mM to 60 mM from 28 to 38 min;Methanol: 18% from 14 to 38 min


Temperature: 30 ˚C

Sample Prep: Diluted 1:10

Inj. Volume: 10 µL

Detections: Suppressed conductivity, ASRS-ULTRA 4 mm,AutoSuppression external water mode

Peaks: 1. Quinate2. Lactate3. Acetate4. Formate5. Galacturonate6. Chloride7. Succinate8. Malate9. Sulfate

10. Oxalate11. Phosphate12. Citrate13. Isocitrate14. cis-Aconitate


Eluent: Sodium hydroxide:1 mM from 0 to 8 min,1 mM to 30 mM from 8 to 28 min,30 mM to 60 mM from 28 to 38 min;Methanol: 0% to 20% from 8 to 18 min,20% from 18 to 28 min, 20% to 0%from 28 to 38 min


Temperature: 30 ˚C

Sample Prep: Diluted 1:10, treated with OnGuard-RP

Inj. Volume: 10 µL


Peaks: 1. Lactate2. Acetate3. Formate4. Pyruvate5. Galacturonate6. Chloride7. Nitrate8. Succinate9. Malate

10. Tartrate11. Fumarate12. Sulfate13. Oxalate14. Phosphate15. Citrate16. Isocitrate17. cis-Aconitate18. trans-Aconitate

Unk = Unknown

Figures 7 and 8 illustrate theseparation of organic acids and inorganicanions in red wine and apple juice usingthe AS11-HC column. The AS11-HCcolumn provides improved resolutionover the AS11 column for componentswith widely varying concentration ratios.The AS11-HC column resolves succinateand malate, even at very differentconcentrations, as shown in Figure 8.

IonPac AS11-HC for Organic Acids andInorganic Anions in FermentationBroths

Monitoring fermentation broths isnecessary for process control, becausemany ingredients resulting frommetabolic processes affect yield orquality of the desired fermentationproducts. Inorganic anions maintainosmolarity and provide co-factors forbiosynthesis. Certain organic acids arewaste products, and their accumulationinhibits cell growth or product yields.

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Figure 9. Separation of common fermentation broth anions using the IonPac AS11-HCcolumn.

14082

Figure 10. Analysis of grape juice on an IonPac AS11-HC column using a potassiumhydroxide gradient delivered by an EG40 Eluent Generator.

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Peaks: 1. Lactate2. Acetate3. Propionate4. Formate5. 2-Keto-D-Gluconate6. Pyruvate7. Valerate8. Monochloroacetate9. Bromate

10. Chloride11. Phenylacetate12. Bromide13. Nitrate14. Malate15. Methylmalonate16. Carbonate17. Malonate18. Maleate19. Sulfate20. Oxalate21. Phosphate22. Citrate23. Isocitrate24. Pyrophosphate25. Trichloroacetate

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Temperature: 30 ˚C

Sample Prep: 1:10 Dilution with deionized water

Detector: Suppressed conductivity,ASRS-ULTRA 4 mm, AutoSuppressionexternal water mode

Peaks: 1. Quinate 55.0 mg/L (ppm)2. Fluoride 0.83. Lactate 46.24. Galacturonate 60.05. Chloride 1.06. Nitrate 0.67. Glutarate 0.78. Succinate 1.09. Malate 116.0

10. Malonate/Tartrate 190.0

11. Maleate 2.012. Sulfate 15.013. Oxalate 12.914. Phosphate 27.015. Citrate 80.016. Iso-Citrate 1.0

The high capacity of the AS11-HCcolumn allows for the determination of anumber of inorganic anions and organicacids in fermentation broths. Figure 9illustrates the separation of commonfermentation broth anions using theIonPac AS11-HC column with a sodiumhydroxide gradient at a controlledtemperature of 30 ˚C.

Gradient Separations as Simple asIsocratic Runs with the EG40 EluentGenerator

The EG40 Eluent Generatorproduces high-purity potassiumhydroxide eluent electrolytically,eliminating the need for eluent prepara-tion. Only a source of deionized wateris required. The potassium hydroxideeluent produced is free of carbonatecontamination. The use of carbonate-free hydroxide eluents minimizesbaseline shifts during hydroxidegradients which provides greaterretention time reproducibility, lowerbackground conductivity, and lowerdetection limits for target analytes.

Figure 10 illustrates the gradientseparation of inorganic anions andorganic acids in grape juice on anIonPac AS11-HC using a potassiumhydroxide gradient delivered by anEG40 Eluent Generator.


Eluent: 0.5–90 mM Sodium hydroxide gradient


Inj. Volume: 10 µL

Temperature 30 ˚C

Sample: 10 mg/L each analyte

Detection: Suppressed conductivity, ASRS-ULTRA 4 mm,AutoSuppression recycle mode


Eluent A: Potassium hydroxide

Eluent B: Sodium hydroxide

Gradient 1 mM from 0 to 8 min, to 30 mMat 28 min, to 60 mM at 38 min;Methanol: 10% from 0–38 min


Inj. Volume: 10 µL

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Figure 12. Determination of anions in 60% stabilized hydrogen peroxide using the IonPacAS11 column.

14146

Figure 11. Analysis of sorbate in cake frosting on an IonPac AS11 column using a potassiumhydroxide gradient delivered by an EG40 Eluent Generator.

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Column: IonPac AG11, AS11, 4 mm

Eluent: Potassium hydroxide,0.5 mM isocratic for 2.5 min,to 5 mM at 6 min, to 38 mMat 18 min

Eluent Source: EG40


Inj. Volume: 25 µL

Detection: Suppressed conductivity, ASRS-ULTRA4 mm, AutoSuppression recycle mode

Peaks: 1. Sorbate 10.5 mg/L (ppm)

Sample Prep: Diluted 1:1000 with deionized water


Inj. Volume: 750 µL


Sample: 60% Hydrogen peroxide(reagent grade, C1), diluted 1:50

Peaks*: 1. Chloride 0.42 mg/L (ppm)*2. Nitrate 22.03. Sulfate 0.744. Phosphate 170.05. Pyrophosphate 33.0

Figure 11 illustrates the use of theIonPac AS11 column with the EG40Eluent Generator for an application ofinterest to the food industry, determina-tion of sorbate, a sweetener used incake frosting. In this example, apotassium hydroxide gradient is used toelute sorbate in less than 6 min and alsoresolve other inorganic anions andorganic acids present in cake frosting.

AS11 for Anions in Hydrogen PeroxideFabricators of electronic compo-

nents use hydrogen peroxide during themanufacturing process. Monitoringchemical reagents for anionic impuri-ties is important because the anioniccontaminants can cause corrosion whenpresent in manufacturing solutions. Amethod has been developed using theAS11 column to determine inorganicanions in stabilized hydrogen peroxide.Figure 12 illustrates the separation ofthe anionic contaminants in hydrogenperoxide using a large-loop injectionwith a sodium hydroxide gradient.

AS11-HC for Trace Anions inMethanesulfonic Acid

Monitoring anionic contaminantsin chemical reagents is an importantquality control function for specialtychemical manufacturers. The high-capacity AS11-HC column is ideal fordetermining trace anions in concen-trated acids. Figure 13 illustrates theseparation of trace chloride and sulfatein concentrated methanesulfonic acidusing the AS11-HC column. Chlorideand sulfate can easily be determineddown to the low ppb level with goodpeak shape even in the presence of highconcentrations of methanesulfonic acid.

Figure 13. AS11-HC for trace anions in concentrated methanesulfonic acid.

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Sample Prep: Diluted 1:1000

Inj. Volume: 25 µL


Peaks: 1. Fluoride 16 µg/L (ppb)2. Unknown —3. Methanesulfonic acid —4. Chloride 375. Sulfate 637

13498

Column: IonPac AS11, 4 mm

ConcentratorColumn: IonPac AG11, 4 mm

Eluent: 1.5 mM Sodium hydroxide, 3 min1.5–40 mM Sodium hydroxidegradient, 13 min

Column: IonPac AG11-HC,AS11-HC, 4 mm

Eluent: Sodium hydroxide:10 mM from 0 to5 min, 10 mM to50 mM from5 to15 min

8510-A

Figure 14. Separation of common inorganic anions, including iodide, perchlorate,thiocyanate, and thiosulfate, using the AS11 column.

8676-05

Figure 15. Separation of polyphosphates in detergent.


Eluent: 45 mM Sodium hydroxidein 40% Methanol


Inj. Volume: 25 µL


Peaks: 1. Fluoride 2 mg/L (ppm)2. Chloride 23. Nitrate 54. Sulfate 55. Phosphate 106. Iodide 207. Thiocyanate 208. Thiosulfate 209. Perchlorate 20

Peaks: A1. Chloride 3 mg/L (ppm)2. Carbonate –3. Sulfate 54. Phosphate 105. Pyrophosphate 106. Trimetaphosphate 107. Tripolyphosphate 108. Tetrapolyphosphate 109. Tetrametaphosphate 10

Unk = Unknown

AS11 Column for Polarizable AnionsThe AS11 column separates

polarizable anions in complex matrices.As shown in Figure 14, for highlyretained hydrophobic anions such asiodide, perchlorate, thiosulfate, andthiocyanate, retention times can besignificantly decreased and peakefficiencies improved by using eluentscontaining organic solvents. Thisapproach is ideal for the determinationof iodide, perchlorate, thiocyanate, andthiosulfate. The IonPac AS16 is recom-mended for this separation without theuse of solvents in the eluent.

AS11 Column for Highly ChargedAnions

The determination of highlycharged anions such as polyphosphates orpolycarboxylates is possible usinggradient elution on a 2-mm or 4-mmIonPac AS11. As shown in Figure 15, thehigh hydroxide selectivity on the IonPacAS11 resin permits elution of these ionsat lower hydroxide concentrations thanare possible on the OmniPac® PAX-100,permitting the use of either 2-mm or4-mm columns. The IonPac AS11column is ideal for the determination ofpolyphosphates, polycarboxylates, andpolysulfonates.

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A Polyphosphate Standard

B Detergent Sample 160 mg/L (ppm)


Eluent: Sodium hydroxide20 mM to 80 mM in 10 min


Detection: Suppressed conductivity,ASRS-ULTRA 4 mm, AutoSuppressionrecycle mode

IonPac AS11 ColumnDimensions:

IonPac AS11 Analytical:2 x 250 mm and 4 x 250 mm

IonPac AG11 Guard:2 x 50 mm and 4 x 50 mm

Maximum Operating Pressure:27 MPa (4000 psi)

Mobile Phase Compatibility:pH 0–14; 0–100% HPLC solvents

Substrate Characteristics:Bead Diameter: 13 µmPore Size: Microporous, <10ÅCrosslinking (%DVB): 55%

Latex Characteristics:Functional Group:Alkanol quaternary ammonium ionLatex Crosslinking: 6%Latex Diameter: 85 nmHydrophobicity: Very Low

Capacity:11 µeq(2 x 250 mm Analytical)45 µeq(4 x 250 mm Analytical)

Column Construction:PEEK with 10–32 threaded ferrule-

style end fittings. All components arenonmetallic.

IonPac AS11-HC ColumnDimensions:

IonPac AS11-HC Analytical:2 x 250 mm and 4 x 250 mm

IonPac AG11-HC Guard:2 x 50 mm and 4 x 50 mm

Maximum Operating Pressure:27 MPa (4000 psi)

Mobile Phase Compatibility:pH 0–14; 0–100% HPLC solvents

Substrate Characteristics:Bead Diameter: 9.0 µmPore Size: 2000ÅCrosslinking (%DVB): 55%

Latex Characteristics:Functional Group:Alkanol quaternary ammonium ionLatex Crosslinking: 6%Latex Diameter: 70 nmHydrophobicity: Medium Low

Capacity*:72.5 µeq(2 x 250 mm Analytical)290 µeq(4 x 250 mm Analytical)

Column Construction:PEEK with 10–32 threaded ferrule-

style end fittings. All components arenonmetallic.

SPEC IF ICAT IONS

* IonPac AG11-HC guard is packed with a microporous, pellicular resin with a capacity of 1.75 µeq (2 x 50 mm guard column) and7 µeq (4 x 50 mm guard column). The latex selectivity is identical to the analytical columns.

Fast profiling of mono-, di-, and trivalent organic acidsand inorganic anions in well-characterized samples AS11High resolution of mono- and divalent organic acids AS11-HCMonovalent organic acids (lactate, acetate, propionate, formate, butyrate) AS11-HCTrace components in complex matrices usinglarge loop injection AS11-HCOrganic acids and inorganic anions in high ionicstrength matrices AS11-HCOrganic acids in high ionic strength matrices(inorganic anions elute in void) ICE-AS6 or ICE-AS1Aromatic acids with conductivity detection AS11Polarizable anions (iodide, thiocyanate, thiosulfate, perchlorate) AS11 or AS16Polyphosphates, polycarboxylates, and polysulfonates AS11 or AS16Preservatives (benzoate, citrate, sorbate) AS11-HC

COLUMN SELECT ION TABLE

Analytes RecommendedColumn

Ordering InformationFor optimum ease-of-use and

economy, the IonPac AS11 andAS11-HC columns should be used withthe ASRS® Anion Self-RegeneratingSuppressor.

When performing sodiumhydroxide gradient anion-exchangeapplications on the AS11 andAS11-HC, an Anion Trap Column(ATC-3) should be installed betweenthe gradient pump and the injectionvalve to remove anionic contaminantsfrom the eluent. The EG40 EluentGenerator is used to automaticallyproduce potassium hydroxide gradientsusing a deionized water source. Whenusing the EG40 Eluent Generator fortrace anion determinations, the ATC-HC column should be installed betweenthe pump outlet and the inlet of theEluGen cartridge.

For 4-mm concentrator work, usethe appropriate guard (IonPac AG11 orAG11-HC) or TAC Concentratorcolumn when a single piston pumpsuch as the DQP or DXP pump is usedfor sample delivery. Use the TAC-LP1Concentrator column when the sampleis delivered with a syringe or with anautosampler such as the AS40 or AS50.For 2-mm concentrator work, use theappropriate guard (IonPac AG11 orAG11-HC) when a single piston pumpsuch as the DQP or DXP pump is usedfor sample delivery.

In the U.S., call (800) 346-6390,or contact the Dionex Regional Officenearest you. Outside the U.S., orderthrough your local Dionex office ordistributor. Refer to the following partnumbers.IonPac AS11 Analytical Column

(4 x 250 mm) ............... P/N 44076

IonPac AG11 Guard Column(4 x 50 mm) ................. P/N 44078

IonPac AS11 Analytical Column(2 x 250 mm) ............... P/N 44077

IonPac AG11 Guard Column(2 x 50 mm) ................. P/N 44079

IonPac AS11-HC Analytical Column(4 x 250 mm) ............... P/N 52960

IonPac AG11-HC Guard Column(4 x 50 mm) ................. P/N 52962

IonPac AS11-HC Analytical Column(2 x 250 mm) ............... P/N 52961

IonPac AG11-HC Guard Column(2 x 50 mm) ................. P/N 52963

ATC-3 4-mm (9 x 24 mm) Anion TrapColumn ........................ P/N 59660(for use with 4-mm columns)

ATC-3 2-mm (4 x 3 5 mm) Anion TrapColumn ........................ P/N 59661(for use with 2-mm columns)

ACT-HC (9 x 75 mm) Anion TrapColumn ......................... P/N 59604(for use with the EG40 EluentGenerator)

IonPac TAC-2 Trace AnionConcentrator(3 x 35 mm) ................. P/N 43101

IonPac TAC-LP1 Trace AnionConcentrator(4 x 35 mm) ................. P/N 46026

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LPN 034827-05 4M 11/01©2001 Dionex Corporation

Dionex Corporation Dionex Corporation Dionex U.S. Regional Offices Dionex International Subsidiaries1228 Titan Way Salt Lake City Technical Center Sunnyvale, CA (408) 737-8522 Austria (01) 616 51 25 Belgium (32) 3-353 42 94 Canada (905) 844-9650 China (852) 2428 3282 Denmark (45) 36 36 90 90P.O. Box 3603 1515 West 2200 South, Suite A Westmont, IL (630) 789-3660 France (01) 39 30 01 10 Germany (06126) 991-0 Italy (06) 66 51 50 52 Japan (06) 6885-1213 The Netherlands (0161) 43 43 03Sunnyvale, CA Salt Lake City, UT Houston, TX (281) 847-5652 Switzerland (062) 205 99 66 United Kingdom (01276) 69172294088-3603 84119-1484 Atlanta, GA (770) 432-8100 * Designed, developed, and manufactured under an NSAI registered ISO 9001 Quality System.(408) 737-0700 (801) 972-9292 Marlton, NJ (856) 596-0600

ASRS, AutoSuppression, OnGuard IonPac, OmniPac, andAnion Self-Regenerating Suppressor are registered trademarks

and MicroBead is a trademark of Dionex Corporation.

ionpac as11 and as11-hc anion-exchange columns · using sodium or potassium hydroxide gradient...

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