internship reort presentation (sajid aslam)
DESCRIPTION
I have described here that work which I have done in 3-months at a Pakistan Government Institution Entomological Research Institute that is under Ayub Agriculture Research Institution, Faisalabad.TRANSCRIPT
IN THE NAME OF ALLAH, THE MOST BENEFICENT AND THE MERCIFUL
INTERNSHIP REPORTPRESENTATION
MUHAMMAD SAJID ASLAM
2009-ag-2138
Introduction to
AARI
ERI
90 Days at AARI
Individual Project
Efficacy of different insecticides on predatory 7-spotted ladybird beetle (Coccinella septempunctata)
INTERNSHIP PLACE (AARI)My internship place was Entomological Research Institute (ERI) at AyubAgriculture Research Institute (AARI),Faisalabad.
The basic purpose of AARI is to introduce new technologies in the field of Agriculture via research activities
AARI is administrated by the Secretary, Government of the Punjab, Agriculture Department, Lahore and headed by the Director General Agri. (Research), Ayub Agricultural Research Institute, Faisalabad. Main campus is located in Faisalabad.
The institute has also its
Research stations, Sub-stations,
Testing centers, Service laboratories
and research cum demonstration farms
Located throughout the province of the Punjab to cater
ecological specific technology needs.
OBJECTIVES OF AARI
The main objectives of this Research Institute are
Improvement of crop varieties.
Development of production technology.
Control of diseases and insect/pests.
Introduction of new industrial/food plants.
Preservation/processing of agricultural products.
Post-Harvest Technology of fruit and vegetable crops.
Farmer’s advisory service.
Utilization of latest research in biotechnology for crop improvement
ENTOMOLOGICAL RESEARCH INSTITUTE (ERI)
In ERI my internship supervisor was and co-supervisor
Mr. Dilbar Hussain Mr. Muhammad Saleem
Entomology Research Institute founded in 1906 as a section of Agriculture College, Layllpur.
When Ayub Agriculture Research Institute is founded in 1962, it became independent Entomological Section.
In 2004, as its marvelous achievements considered, it is upgraded to a new institute of A.A.R.I.
OBJECTIVES OF ERI
Survey and identification of useful and harmful insects and mites ofcrops, fruits, Vegetables and stored grains in different areas of theProvince.
Development of effective and economical control measures.
Development of taxonomy, ecology and biology of major insect pestsand mites.
Determination of magnitude of qualitative and quantitative food/croplosses.
Screening of varieties of main crop against major insects pests and mitesto find out Resistant/high yielding varieties.
Testing of new pesticides against major insect pests of agricultural cropfor Standardization and recommendation.
Guidance for the farmers in diagnosing the insect pest problems andtheir control Measures.
Disseminate knowledge gained through research activities throughelectronic and print Media.
LABORATORIES OF ERIINSECTICIDE RESISTANCE LABORATORY
TRICHOGRAMMA REARING LABORATORY
CRYSOPERLA CARNEA REARING LAB
STORED GRAIN LABORATORY
COTTON MEALY BUG RESEARCH LABORATORY
90 DAYS AT AARI Reception and Introduction
Field Visits
Sunflower Maize
Chickpea Cotton
Wheat Onion
Tomato Cabbage
Individual Project
Efficacy of different insecticides on predatory 7-spotted ladybird beetle (Coccinella septempunctata)
INDIVIDUAL PROJECT
WHEAT
Wheat is staple food in Pakistan
Its grow on maximum land of overall Pakistan
Mainly its damage by aphid.
Aphid population can be control by taking some measures
• Biological control
Ladybird beetle is most effective biological control in wheat crop pests.
Triticum aestivum
By ProductsBreads, pastries, pan-cake, cake
flours, cookie dough, pies, croutons,
pizza, dough, cakes, ice cream, ice
cream cones
Processed cheese spreads and
cheese food, egg noodles, whiskey,
beer
Chewing gum
INTRODUCTION
Ladybird beetle is most effective biological control against wheat aphid.
But now a days excessive use of pesticides directly or indirectly affecting its population and causing decrease in its ability to prey
Its larvae and adult both feed on aphid. So that’s why its most efficient biological control of aphid in wheat
Conservation of LBB is necessary now-a-days to sustain its population
By keeping in mind this purpose
To check % mortality in the ladybird beetle, I conduct a trial (Bioassay) against 4 different insecticides
MATERIALS AND METHODSThe study was conducted at Insecticide Resistance Lab inthe Entomology Research Institute (ERI) of Ayub AgricultureResearch Institute (AARI) during internship period 2013. Theexperiment was laid out in complete randomize design(CRD) followed by five treatments of each insecticide withFive replications and 2 treatment with water as controlled.First of all i collected the larvae of 7-spotted ladybird beetlefrom the wheat crop at AARI and rear them in lab at 25 + 2oC and 60-65 % Relative humidity until adults emerged.
Materials: Jars, camel hair brush, petridishes, beakers(500ml), pipette, stirrer, fore-sap, sunflowerleaf, leaf cutter, insecticides
INSECTICIDES USED
Sr. no Active Ingredient Trade Name/Formulation Dose(ml/acre)
1 Imidachloprid Confidor 20SL 250
2 Acetameprid Moziban 20SL 145
3 Emamectin Benzoate Emamectin 1.9EC 200
4 Spinosad Tracer 240SC 80
STEP WISE PROCEDURE
MATERIALS AND METHODS
The data was recorded after 3 hours, 6 hours, 12 hours, 24 hours and 48 hours. The percentage mortality in each treatment by sticking with a formula
Number of insect died
Percent Mortality = ------------------------------------------ x 100
Total number of insect present
The calculated data was subjected to Probit Analysis for the determination of LC50 & LC90.
Time LC50 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.L X2
3 9705.97 6558.83 9177.5 0.021+0.19 1.8 0.59 0.021-1.21X
6 8618.84 5650.47 6224.6 0.011+0.17 1.22 0.73 0.011-0.93X
12 7139.3 4569.74 4633.9 0.009+0.16 0.95 0.8 0.009-0.71X
24 5430.36 3239.15 3394.8 0.019+0.16 0.8 0.846 0.019-0.51X
48 3613.94 883.304 957.3 0.012+0.15 0.71 0.868 0.012-0.33X
LC50 value and regression analysis of different concentrations of Imidachloprid Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
Time LC90 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.LX2
3 19903.7 12614.3 63909.3 0.021+0.19 1.8 0.59 0.021-1.21X
6 20393.1 12420.2 13223.2 0.011+0.17 1.22 0.73 0.011-0.93X
12 19981.3 11887.1 13109.1 0.009+0.16 0.95 0.8 0.009-0.71X
24 18895.6 11117.4 12243 0.019+0.16 0.8 0.846 0.019-0.51X
48 17285.9 10166 15667 0.012+0.15 0.71 0.868 0.012-0.33X
LC90 value and regression analysis of different concentrations of Imidachloprid Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
33 40 47 53 60
2633
4047
54
20
27
33
40
47
13
20
27
33
40
7
14
20
27
34
0
50
100
150
200
250
3 h 6 h 12 h 24 h 48 h
Mo
rta
lity
(%
)
TIME
Imidachloprid
7000 3500 1750 875 437.5
% Mortality of adults of Coccinella septempunctata against different concentration of
Imidachloprid after different exposure periods.
Time LC50 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.L X2
3 324.886 115.847 175.935 0.3+0.45 0.048678 0.009 0.3-1.75X
6 197.173 77.9531 138.956 0.27+0.42 0.015784 0.015 0.27-1.43X
12 106.446 46.8524 209.538 0.25+0.41 0.004475 0.019 0.25-1.18X
24 53.9296 19.8639 81.7423 0.24+0.40 0.000602 0.021 0.24-0.98X
48 26.7756 1.76653 7.6948 0.24+0.39 0.000602 0.021 0.24-0.80X
LC50 value and regression analysis of different concentrations of Emamectin Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
Time LC90 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.L X2
3 22245.5 1445.71 1545.31 0.3+0.45 0.048678 0.009 0.3-1.75X
6 22349 1279.94 1324.77 0.27+0.42 0.015784 0.015 0.27-1.43X
12 16655 1004.32 1122.15 0.25+0.41 0.004475 0.019 0.25-1.18X
24 9879.61 721.141 823.04 0.24+0.40 0.000602 0.021 0.24-0.98X
48 4905.15 482.294 552.60 0.24+0.39 0.000602 0.021 0.24-0.80X
LC90 value and regression analysis of different concentrations of Emamectin Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
40 47 53 60 67
3340
4753
6027
3440
4753
20
27
33
40
47
13
20
27
33
40
0
50
100
150
200
250
300
3 h 6 h 12 h 24 h 48 h
MO
RTA
LITY
(%
)
TIME
Emamectin
152 76 38 19 9.5
GRAPHICAL REPRESENTATION
% Mortality of adults of Coccinella septempunctata against different
concentration of Emamectin after different exposure periods.
Time LC50 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.LX2
3 2566.21 897.812 980.271 0.32+0.69 0.088818 0.007 0.32-2.58X
6 1696.54 632.223 724.3250 0.28+0.64 0.027663 0.012 0.28-2.11X
12 963.591 405.286 640.399 0.26+0.61 0.008739 0.017 0.26-1.79X
24 501.014 218.225 298.177 0.24+0.60 0.001945 0.02 0.24-1.54X
48 250 51.9877 62.0221 0.24+0.59 0.000181 0.021 0.24-1.35X
LC50 value and regression analysis of different concentrations of Acetamiprid Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
Time LC90 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.LX2
3 126250 9490.5 9838 0.32+0.69 0.088818 0.007 0.32-2.58X
6 153146 9111.74 9835 0.28+0.64 0.027663 0.012 0.28-2.11X
12 131091 7553.96 8236 0.26+0.61 0.008739 0.017 0.26-1.79X
24 86533.7 5653.8 3605 0.24+0.60 0.001945 0.02 0.24-1.54X
48 46703.5 3911.57 4381 0.24+0.59 0.000181 0.021 0.24-1.35X
LC90 value and regression analysis of different concentrations of Acetamiprid Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
37 43 50 57 6430
3744
5057
2430
3643
50
17
23
30
37
43
10
17
24
30
37
0
50
100
150
200
250
300
3 h 6 h 12 h 24 h 48 h
MO
RTA
LITY
(%
)
TIME
ACETAMEPRID
152 76 38 19 9.5
% Mortality of adults of Coccinella septempunctata against different concentration of
Acetamiprid after different exposure periods.
Time LC50 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.L X2
3 47523.2 15523.7 17592 0.33+1.06 0.706594 0.008 0.33-3.58X
6 41393.2 12512.7 12634.9 0.27+0.97 0.363142 0.019 0.27-2.88X
12 20957.7 15971 18216 0.27+0.93 0.599079 0.014 0.27-2.73X
24 8228.32 3625.9 3688 0.25+0.89 0.447261 0.931 0.25-2.25X
48 3567.92 372.356 419.315 0.23+0.88 0.100311 0.028 0.23-1.90X
LC50 value and regression analysis of different concentrations of Spinosad Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
Time LC90 F.L Slop +S.E Chi-Square P Regression line
(Hours) L.F.L U.F.L X2
3 2229398 155692 159999 0.33+1.06 0.706594 0.008 0.33-3.58X
6 4658956 183711 193734 0.27+0.97 0.363142 0.019 0.27-2.88X
12 2236454 130283 135676 0.27+0.93 0.599079 0.014 0.27-2.73X
24 1382271 89251.2 89867.8 0.25+0.89 0.447261 0.931 0.25-2.25X
48 882879 62599.6 62899.9 0.23+0.88 0.100311 0.028 0.23-1.90X
LC90 value and regression analysis of different concentrations of Spinosad Insecticide against adults of 7-spotted lady bird beetle (Coccinella septempunctata)
33 40 47 57 6427
3037
4757
2327
34
4450
1724
30
40
47
7
14
17
27
37
0
50
100
150
200
250
300
3 h 6 h 12 h 24 h 48 h
MO
RTA
LITY
(%
)
TIME
SPINOSAD
152 76 38 19 9.5
% Mortality of adults of Coccinella septempunctata against different
concentration of Spinosad after different exposure periods.
CONCLUSION
Imidachloprid is safer for ladybird beetles because it hasminimum mortality 60% at its maximum ppm concentrationagainst maximum exposure time
While, Acetameprid and Spinosad are moderately toxic64%) against their maximum ppm concentration atmaximum exposure time
Emamactin is highly toxic to ladybird beetle, it has causesmaximum mortality (67%) as compare to other insecticides
REFERENCES
• Sathe, T.V. and Y.A. Bhosale, 2001. Insect pest predators. Daya publishing house, Delhi, pp: 124.
• Chowdhury, S.P., M.A. Ahad, M.R. Amin, M.R. Amin and N.A. Rasel, 2008. Bean aphid predation efficiency of lady bird beetle, Micraspis discolor F. (Coleoptera: Coccinellidae). J. Soil Nature, 2(3): 40-45.
• Hassan, M.N., 2002. Evaluation of commercially available insecticides on ladybird beetls. M.S. thesis. Department of Entomology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur.
• Ganapathy, N. and C. Durairaj, 1995. Contact toxicity of insecticides to Menochilus sexmachulatus. Indian J. Pulses Res., 8(2): 197-198.
• DeBach, P., 1964. Biological control of insect pests and Weeds. Chapman and Hall ltd., New Peter Lane, London, pp: 843.
• Zeb, Q., H. Badshah, H. Ali, R.A. Shah and M. Rehman. 2011. Population of aphids on different varieties/lines of wheat and their effect on yield and thousands grain weight. Sarhad J. Agric. 27(3): 443-450.
• He, Y., J. Zhao, Y. Zheng, N. Desneux and K. Wu, 2012. Lethal effect of imidacloprid on the coccinellid predator Serangium japonicum and sublethal effects on predator voracity and on functional response to the whitefly Bemisia tabaci. Ecotoxicol., 21(5): 1291-1300.
MUHAMMAD SAJID ASLAM